CN104762277B - 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 - Google Patents
糖基化改造提高脂肪酶表达的方法、突变酶及其应用 Download PDFInfo
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- CN104762277B CN104762277B CN201510195799.9A CN201510195799A CN104762277B CN 104762277 B CN104762277 B CN 104762277B CN 201510195799 A CN201510195799 A CN 201510195799A CN 104762277 B CN104762277 B CN 104762277B
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/845—Rhizopus
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Abstract
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Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510195799.9A CN104762277B (zh) | 2015-04-22 | 2015-04-22 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
PCT/CN2016/070236 WO2016169306A1 (zh) | 2015-04-22 | 2016-01-06 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
Applications Claiming Priority (1)
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CN201510195799.9A CN104762277B (zh) | 2015-04-22 | 2015-04-22 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN104762277A CN104762277A (zh) | 2015-07-08 |
CN104762277B true CN104762277B (zh) | 2017-07-14 |
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CN201510195799.9A Active CN104762277B (zh) | 2015-04-22 | 2015-04-22 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
Country Status (2)
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CN (1) | CN104762277B (zh) |
WO (1) | WO2016169306A1 (zh) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104762277B (zh) * | 2015-04-22 | 2017-07-14 | 江南大学 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
CN106967737A (zh) * | 2017-03-22 | 2017-07-21 | 东莞泛亚太生物科技有限公司 | 用于生产具有高活性脂肪酶的基因 |
CN108841805B (zh) * | 2018-06-29 | 2021-07-06 | 深圳市夏盟生物科技有限公司 | 一种热稳定性提高的脂肪酶突变体 |
CN108841806B (zh) * | 2018-06-29 | 2021-07-06 | 深圳市夏盟生物科技有限公司 | 一种热稳定性提高的脂肪酶突变体 |
CN108841807B (zh) * | 2018-06-29 | 2021-09-17 | 广东丰绿源生物医药科技有限公司 | 一种热稳定性提高的脂肪酶突变体 |
CN111944783B (zh) * | 2018-12-13 | 2021-09-07 | 浙江大学 | 一种热稳定性提高的脂肪酶突变体及其应用 |
CN109929822B (zh) * | 2019-04-10 | 2021-04-06 | 浙江工业大学 | 一种米曲霉脂肪酶突变体及其应用 |
CN116606836A (zh) * | 2023-07-05 | 2023-08-18 | 广东博创佳禾科技有限公司 | 一种酶活、热稳定性提高的脂肪酶突变体 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101857856B (zh) * | 2010-05-25 | 2012-02-01 | 中国科学院武汉病毒研究所 | 一种枯草芽孢杆菌脂肪酶及制备方法和应用 |
CN102363786B (zh) * | 2011-10-17 | 2013-12-11 | 中国水产科学研究院黄海水产研究所 | 一种适冷性海洋酵母Bohai Sea-9145低温碱性脂肪酶基因、氨基酸序列及重组脂肪酶 |
CN104762277B (zh) * | 2015-04-22 | 2017-07-14 | 江南大学 | 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 |
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2015
- 2015-04-22 CN CN201510195799.9A patent/CN104762277B/zh active Active
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2016
- 2016-01-06 WO PCT/CN2016/070236 patent/WO2016169306A1/zh active Application Filing
Non-Patent Citations (4)
Title |
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role of N-linked glycosylation in the secretion and activity of endothelial lipase;Gwen C.Miller et al.;《journal of lipid research》;20041231;第45卷;2080-2085 * |
role of n-linked glycosylation in the secretion and enzymatic properties of phizopus chinensis lipase expressed in pichia pastoris;min yang et al.;《microbial cell factories》;20150321;40-54 * |
米根霉脂肪酶基因prorol在毕赤酵母中的高效表达;郭勇亮等;《工业微生物》;20120430;第43卷(第2期);22-24 * |
米黑霉脂肪酶在毕赤酵母中的表达及分子改造;刘悦;《中国优秀硕士学位论文全文数据库 基础科学辑》;20140615(第6期);A006-84 * |
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Publication number | Publication date |
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WO2016169306A1 (zh) | 2016-10-27 |
CN104762277A (zh) | 2015-07-08 |
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Denomination of invention: Glycosylation modification for improving lipase expression, mutated enzymes, and their applications Effective date of registration: 20230721 Granted publication date: 20170714 Pledgee: Jiangsu Changjiang Commercial Bank Co.,Ltd. Taixing Branch Pledgor: JIANGSU YIMING BIOLOGICAL CO.,LTD. Registration number: Y2023980049356 |
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