CN104756960B - Large-speckle small yellow bee breeding method - Google Patents
Large-speckle small yellow bee breeding method Download PDFInfo
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- CN104756960B CN104756960B CN201510214638.XA CN201510214638A CN104756960B CN 104756960 B CN104756960 B CN 104756960B CN 201510214638 A CN201510214638 A CN 201510214638A CN 104756960 B CN104756960 B CN 104756960B
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Abstract
The invention discloses a large-speckle small yellow bee breeding method. The method includes the following steps that healthy rice leaf folder larvae are collected from rice paddies，imagoes are fed indoors, or collected directly from rice paddies; after copulation of the female and male imagoes, female adults are transferred to rice seedlings which are put in bee cultivating cage so that eggs can be laid, and larvae can be fed with rice leaves directly after the eggs are hatched; the rice leaf folder larvae or large-speckle small yellow bee pupas, which are parasitized by the large-speckle small yellow bee collected from field, are contained in a finger-shaped tube and taken to a lab, and after being formed after eclosion, the large-speckle small yellow bee imagoes are fed with hydromel; large-speckle small yellow bee female adults and large-speckle small yellow bee male adults are paired, mated large-speckle small yellow bees are transferred to a bee cultivating cage which have rice seedlings and rice leaf folder larvae, and enabled to perform free parasitism on the rice leaf folder larvae and be fed with them, when the rice leaf folder larvae are parasitized by the large-speckle small yellow bee, the parasitized larvae are put back to the finger-shaped tube, and humidity-preserving cultivation is performed till mature large-speckle small yellow bee larvae climb away from the rice leaf folder larvae. The large-speckle small yellow bee mature larvae are collected, and humidity-preserving cultivation is performed till the large-speckle small yellow bee larvae are turned into the pupas and eclosion, the large-speckle small yellow bee adults of eclosion can be used to the parasitism of rice leaf folder larvae. The method is suitable for artificially breeding the large-speckle small yellow bees, the survival rate of the bred large-speckle small yellow bees is high, the large-speckle small yellow bees do not carry other diseases or other bacteria and the method provides a guiding effect for biological prevention and control of rice leaf folders.
Description
Technical field
This technology belongs to biological raising field and in particular to the method for breeding of big speckle Huang chalcid fly, can be used for the biology of insect
Prevention and control field.
Background technology
Means due to preventing and treating migratory pest rice leaf roller at this stage are mainly chemical prevention, but pesticide apply,
Lavishment, frequently results in environmental pollution, kills natural enemy, pest resistance to insecticide and rampant again.Most of Pesticide use persons have ignored field
There is substantial amounts of pest natural enemy, the especially presence of parasitic wasp.For example, total parasitic rate of Xinjiang parasitic wasp in 1978 is in 33-
Between 90%.Big speckle Huang chalcid fly is 39.22% (yellow show branch etc., 2013) to the highest parasitic rate of rice leaf roller in the first half of the year.
In nearly 30 years, many parasites and predator are all identified, in Asia parasitic wasp more than 100 kinds of (He and
Chen 1990；Ma et al.2002；GuizhouAgricultural science and technology Committee
1986；Lou et al.2014).However, seldom having any cnaphalocrocis being used to preventing and treating field in these parasitic wasps
Snout moth's larva, they are the monoid of naturally occurring in the middle of ecosystem, also do not rise to by the mankind dedicated for rice leaf roller
In preventing and treating.Raising for rice leaf roller parasitic wasp also seldom touches.So, a kind of advantage of research rice leaf roller is parasitic
The rearing method of honeybee, may provide technological guidance for the Biological control of rice leaf roller.
Content of the invention
An object of the present invention is the raising for solving above-mentioned shortage rice leaf roller parasitic wasp big speckle Huang chalcid fly
Method, that is, provide a kind of can artificial breeding's big speckle Huang chalcid fly method.
A kind of big speckle Huang chalcid fly method for breeding, comprises the following steps：
(1) gather rice leaf roller：From the rice leaf roller larvae of rice terrace collection health, raise indoors to adult,
Or directly gather rice leaf roller adult from field；
(2) raise rice leaf roller：Allowing female male imago to put in insect cage makes its copulation, and the female adult of post-coitum is transferred to
Lay eggs on rice seedlings in insect cage, 3 day after tomorrow will be driven female adult worm out of in spawning；Ovum is allowed freely to hatch on blade as larva；Rice indulges volume
Leaf snout moth's larva larva directly takes food rice leaf, and continued growth is developed until aging, and collection rice leaf roller mature larva is to dactylethrae
In, treat that it is pupated；Pupa directly discharges rice leaf roller adult on the rice seedlings having been placed in insect cage so as to produce after sprouting wings
Ovum, makes rice leaf roller population continued propagation indoors；
(3) collection big speckle Huang chalcid fly：To gather from field by the parasitic rice leaf roller larvae or big of big speckle Huang chalcid fly
The pupa of speckle Huang chalcid fly, loads in dactylethrae and takes back laboratory, the dactylethrae plug with air-vent beyond the Great Wall；Treat the yellow little bee imago of big speckle
The hydromel using 5%-10% after emergence is fed；
(4) raise big speckle Huang chalcid fly：Dip in the yellow little bee imago of the big speckle of 5%-10% hydromel feeding with sterilized absorbent cotton, then
Make the yellow little bee imago male and female pairing of big speckle, the big speckle Huang chalcid fly female adult worm of post-coitum is transferred in the insect cage being placed with rice seedlings,
Raising on rice seedlings in insect cage has 3 age rice leaf roller larvaes, allows big speckle Huang chalcid fly female adult worm parasitism cnaphalocrocis naturally
Snout moth's larva 3 instar larvae, after parasitic 3 days, will be recovered in dactylethrae by parasitic rice leaf roller larvae, and moisturizing is cultivated, and gives daily
Rice leaf roller larvae changes fresh paddy rice blade, until big speckle Huang chalcid fly mature larva is climbed from rice leaf roller larvae；Collect
Big speckle Huang chalcid fly mature larva, for its moisturizing, treats that it is pupated；The big speckle Huang chalcid fly sprouting wings then continues to allow its parasitic rice leaf roller
3 instar larvaes, thus carry out the breeding of big speckle Huang chalcid fly population of future generation；
In the feeding process of rice leaf roller each worm state and in the feeding process of big speckle Huang chalcid fly each worm state, set indoor
Raising temperature is 25 DEG C～27 DEG C, and humidity is between 70%～80%.
The beneficial effects of the present invention is：The invention provides a kind of method of suitable artificial breeding big speckle Huang chalcid fly, should
The big speckle Huang chalcid fly survival rate that method is raised is high, no carries other diseases or miscellaneous bacteria, the Biological control for rice leaf roller provides
Directive function.
Specific embodiment
Describe present disclosure below in conjunction with specific embodiment in detail.It should be noted that retouching in following embodiments
The technical characteristic stated or the combination of technical characteristic are not construed as isolated, and they can be mutually combined thus reaching
Superior technique effect.
Embodiment 1
1st, breeding facility
1.1 insect cage
Big insect cage (length × width × height=1m × 1m × 1.5m), with rustless steel as support, five faces are that ventilation and penetrating light is good
Gauze, before for two fan open the door, bottom surface be plastic septum.Little experimental cage (length × width × height=30cm × 30cm × 50cm) material
Matter is consistent with big insect cage with structure.
1.2 pot rice basins
Specification is diameter 20cm, the Plastic Drum of high 25cm.
1.3 other materials
Dactylethrae, plastics plug, label paper, rubber band, gobo (black cloth), Cotton Gossypii of sterilizing, pin, Mel mixed liquor
(being diluted to 10%), pallet, clap net.
2nd, rice cultivation
Rice varieties are that wild silk accounts for and carries out potted plant.Rice seed is chosen seeds, is rejected empty, flat, miscellaneous, rotten paddy, choosing
Seed divide on pallet, add water to the water surface and just do not had whole seeds, be dipped to seed through a couple of days and expose white bud, then
After ten a few houres, treat that bud is up to 0.2cm, the seed of germination is spread out in seedling disk, covers thin soil lid toward seed, water to not having
Live soil layer.Make seed growth.After rice shoot grows to 20 centimetres high, then by transplantation of seedlings in plant pot, more potted plant put into all
Insect cage.After transplanting one week, to fertilization of rice.Continue to employ.
3rd, collection, raising rice leaf roller
Select the rice terrace of non-dispenser, therefrom gather the rice leaf roller larvae of health, catch cnaphalocrocis with sweeping worm net and sweeping
Snout moth's larva adult, the larva that collection is returned carries out indoor feeding to adult, and the adult collecting or the adult of indoor feeding are directly put
Enter in little experimental cage so as to copulation, then the female adult of post-coitum is transferred to its Free-spawning allowed on the rice seedlings planted, 3 days
Afterwards adult is driven out of, allow ovum freely to hatch on blade.Rice leaf roller larvae after hatching grows to 3 on pot rice
In age, continue to employ, wait the yellow little bee imago of big speckle waiting.
4th, collection, raising big speckle Huang chalcid fly
From field collection by the pupa of the parasitic rice leaf roller larvae of big speckle Huang chalcid fly and big speckle Huang chalcid fly, load dactylethrae
In, the plug with air-vent (need to be changed fresh paddy rice blade daily by parasitic rice leaf roller larvae, treat that big speckle Huang is little beyond the Great Wall
Honeybee mature larva is climbed from rice leaf roller larvae, and the same day or next day pupate；The yellow little Pupa Apis of big speckle adopted are without feeding Rice Leaf
Piece), wait the yellow little Pupa Apis of big speckle to turn into adult, dip in upper 10% dilution hydromel with sterilized absorbent cotton and fed.
By the Huang chalcid fly female male worm pairing of big speckle, the big speckle Huang chalcid fly after pairing is transferred to equipped with raising 3 age rice leaf roller
In the insect cage of the rice seedlings of larva, the rice leaf roller larvae taking food allowed on its naturally parasitic Oryza sativa L., after parasitic 3 days, will
It is recovered in dactylethrae by the parasitic rice leaf roller larvae of big speckle Huang chalcid fly, moisturizing is cultivated, daily for rice leaf roller larvae
Change fresh blade, until big speckle Huang chalcid fly mature larva is climbed from rice leaf roller larvae.Collect big speckle Huang chalcid fly mature larva,
Treat that it is pupated.
The yellow little bee imago of big speckle sprouted wings then continues to allow its parasitic rice leaf roller 3 instar larvae, thus starting of future generation big
The breeding of speckle Huang chalcid fly.In big speckle Huang chalcid fly adult stage, 5%-10% hydromel to be fed in time and be put into cnaphalocrocis
On the rice seedlings of snout moth's larva 3 instar larvae.
5th, the test of rice leaf roller each instar larvae parasitic rate and result
15 basin health pot rices are placed, every three basins are one group, so that each group Oryza sativa L. phase is not in contact with each other in each insect cage.Point
With label paper, every group of Oryza sativa L. basin not being numbered is 1,2,3,4,5, suitable according to each age for each instar larvae of rice leaf roller raised
Sequence is connected on the rice leaf of reference numeral (i.e. respectively：No. 1 is first-instar young, and No. 2 is second instar larvae, and No. 3 is third-instar larvae, 4
Number be four-age larva, No. 5 be five-age larva).Every cage is a repetition, totally three repetitions.15 rice are put on every three basin Oryza sativa L. indulge
Leaf roll snout moth's larva larva, every cage puts 10 big speckle Huang chalcid flies, and (sex ration is about 1:3).Close cage door, in case big speckle Huang chalcid fly run out of and
Other insecticide interference tests, keep paddy growth ventilation and penetrating light simultaneously.After 3 days, open insect cage, big speckle Huang chalcid fly driven out of,
And each instar larvae of rice leaf roller participating in test is gathered go back to interior one by one, raised with Rice Leaf in dactylethrae, daily all
Change fresh paddy rice blade, keep room temperature 25-27 DEG C, keep the humidity of 75-80% in dactylethrae.Result of the test is that big speckle is yellow
The parasitic rate to different larval instar rice leaf roller larvae for the chalcid fly：Two age parasitic rates are 16.67%, and three age parasitic rates are 30.23%,
Four age parasitic rates are 15.56%, and five age parasitic rates are 2.78%, do not find that first-instar young is developed to and pupate or bring out big speckle Huang
The data of chalcid fly.The parasitic rate highest to 3 age rice leaf roller larvaes for the yellow little bee imago of speckle greatly.Under suitable humiture, greatly
Speckle Huang chalcid fly departs from host from success parasitism to mature larva and normally pupates, and its ratio is very high, can normally pupate and sprout wings.
Although having been presented for some embodiments of the present invention herein, it will be appreciated by those of skill in the art that
Without departing from the spirit of the invention, the embodiments herein can be changed.Above-described embodiment is exemplary, no
Should be using the embodiments herein as the restriction of interest field of the present invention.
Claims (1)
1. a kind of big speckle Huang chalcid fly method for breeding is it is characterised in that comprise the following steps：
(1) gather rice leaf roller：From the rice leaf roller larvae of rice terrace collection health, raise to adult indoors, or directly
Connect and gather rice leaf roller adult from field；
(2) raise rice leaf roller：Female male imago is put into makes its copulation in insect cage, the female adult of post-coitum is transferred to foster worm
Lay eggs on rice seedlings in cage, 3 day after tomorrow will be driven female adult worm out of in spawning；Ovum is allowed freely to hatch on blade as larva；Rice leaf roller
Larva directly takes food rice leaf, and continued growth is developed until aging, and collection rice leaf roller mature larva, in dactylethrae, is treated
It is pupated；Pupa directly discharges rice leaf roller adult on the rice seedlings having been placed in insect cage so as to spawning after sprouting wings, and makes
Rice leaf roller population continued propagation indoors；
(3) collection big speckle Huang chalcid fly：Yellow by the parasitic rice leaf roller larvae of big speckle Huang chalcid fly or big speckle by gathering from field
The pupa of chalcid fly, loads in dactylethrae and takes back laboratory, the dactylethrae plug with air-vent beyond the Great Wall；Treat big speckle Huang chalcid fly adult eclosion
The hydromel using 5%-10% afterwards is fed；
(4) raise big speckle Huang chalcid fly：Dip in the yellow little bee imago of the big speckle of 5%-10% hydromel feeding with sterilized absorbent cotton, then make big
The yellow little bee imago male and female pairing of speckle, the big speckle Huang chalcid fly female adult worm of post-coitum is transferred in the insect cage being placed with rice seedlings, foster worm
Raising on rice seedlings in cage has rice leaf roller 3 instar larvae, allows big speckle Huang chalcid fly female adult worm naturally parasitic rice leaf roller 3 age
Larva, after parasitic 3 days, will be recovered in dactylethrae by parasitic rice leaf roller larvae, and moisturizing is cultivated, and indulges volume to rice daily
Leaf snout moth's larva larva changes fresh paddy rice blade, until big speckle Huang chalcid fly mature larva is climbed from rice leaf roller larvae；Collect big speckle yellow
Chalcid fly mature larva, for its moisturizing, treats that it is pupated；The big speckle Huang chalcid fly sprouting wings then continues to make its parasitic rice leaf roller 3 age young
Worm, thus carry out the breeding of big speckle Huang chalcid fly of future generation；
In the feeding process of rice leaf roller each worm state and in the feeding process of big speckle Huang chalcid fly each worm state, set indoor feeding
Temperature is 25 DEG C～27 DEG C, and humidity is between 70%～80%.
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| CN102125002B (en) * | 2011-04-13 | 2013-05-29 | 中国农业科学院植物保护研究所 | Method for artificially raising plant bug parasitic wasps |
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