CN104749118B - Variable intervenes formula biomarker concentration detection method and device - Google Patents
Variable intervenes formula biomarker concentration detection method and device Download PDFInfo
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- CN104749118B CN104749118B CN201510131626.0A CN201510131626A CN104749118B CN 104749118 B CN104749118 B CN 104749118B CN 201510131626 A CN201510131626 A CN 201510131626A CN 104749118 B CN104749118 B CN 104749118B
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
The present invention relates to biomarker detection field, more specifically to variable intervenes formula biomarker concentration detection method and device, and optical receiver module receives light beam and forms spectrum, and exports electric signal Qn, sets its zero Q0；It introduces and intervenes variables A n, adjust the position of spectrum formed on optical receiver module, while adjust the electric signal output value of optical receiver module, one is intervened the electric signal output value Qn that variate-value An corresponds to an optical receiver module；The correspondence of variables A n and biomarker concentration Bn in detectable substance are intervened in setting, and variables A n to Am is intervened in adjustment so that the corresponding biomarker concentration Bm of variables A m are intervened in Qn=Q0, inquiry, obtain the concentration of biomarker in detectable substance；N, m be natural number, n>M, the present invention also provides corresponding devices, can easily and efficiently obtain the effective information of biomarker in detectable substance.
Description
Technical field
The present invention relates to biomarker detection field, more specifically to variable intervenes the inspection of formula biomarker concentration
Survey method and device.
Background technology
Biomarker (Biomarker) refers to can be with tagging system, organ, tissue, cell and subcellular structure or work(
The change of energy or the biochemical indicator of change that may occur, have very extensive purposes.Biomarker is examined available for disease
Break, judge staging or for evaluating the safety and efficacy of new drug or new treatment in target group.It is given birth in detection
During object marker, surface plasma resonance technology SPR (Surface Plasmon Reson × ance) is commonly used, that is, utilizes gold
A kind of physical optics phenomenon caused by belonging to the total reflection connection of film/liquid level interface light analyzes bio-molecular interaction, and lead to
It crosses infrared light combination SPR technique and measures biomarker concentration, specifically can refer to paper, " surface plasma resonance technology is in biology
Application in medicine " -2004 grades of Peking University's biomedical engineering Zhang Rui Bin (10403048) and paper " surface plasma
Application and its progress of the resonance body immunosensor in protein detection "-《Analytical chemistry》7th phase in 2010
1052-1059。
In biomarker detection field, photometric method is more common method, by vitro by human body
The component of humoral specimen is mixed with one or more testing reagents, and repeatedly the concentration of determining analyte, thus causes bioid
Reaction is learned, this causes the optical characteristics of measured object that measurable variation occurs, and photometric method detects and light stream is utilized to pass through extinction
The decrease of generation during property and/or astigmatism medium, however, this measuring method is due to by sample type in itself and its
The influence of interference substance that may be included leads to the easy generating system mistake when measuring so as to the concentration of biomarker
Measurement result it is not accurate enough, moreover, measure when light stream direction adjusting be difficult control, often brought greatly to gauger
Puzzlement.As shown in fig. 1, optical detection unit 700 (will use optical receiver module in the present invention) is being used to receive sensing chip
When locating the light beam projected, the spectrum with dark space can be formed above, can export electric signal simultaneously, when being passed through in flow channel
Substance difference when, the position of spectrum can be different with the presence of biomarker in substance, this location information is very difficult
To measure what is determined.
Invention content
In order to solve the above technical problem, the present invention provides biomarker in a kind of quick, accurate acquisition determinand is dense
The variable of degree intervenes formula biomarker concentration detection method, device.
The used to solve above-mentioned technical problem technical solution of the present invention is：Variable intervenes the inspection of formula biomarker concentration
Survey method, includes the following steps, optical receiver module receives light beam, forms spectrum, and export electric signal Qn, sets its output
The zero Q0 of electric signal；It introduces and intervenes variables A n, adjust the position of spectrum formed on optical receiver module by intervening variable, together
When adjust the electric signal output value of optical receiver module, each intervene variate-value An correspond to an optical receiver module electric signal it is defeated
Go out value Qn；The correspondence of variables A n and biomarker concentration Bn in detectable substance are intervened in setting, and variables A n to Am is intervened in adjustment,
Cause Qn=Q0, the corresponding biomarker concentration Bm of variables A m are intervened in inquiry, obtain the concentration of biomarker in detectable substance；
Above-mentioned, n, m are natural number, and n>m.
Formula biomarker concentration detection method is intervened for the variable in the present invention, it is described as further setting
Intervention variables A n=A+n × a, wherein, A is definite value, and a is the single adjustment amplitude for intervening variable, and n is natural number.
Formula biomarker concentration detection method is intervened for the variable in the present invention, it can as one kind in practical measure
Situation about can occur, for biomarker concentration there are subtle variation, the optical receiver module is defeated in measured determinand
The intervention variate-value Am and the concentration value Bm of biomarker of gained gone out after the zero Q0 of electric signal, adjustment is section
Value.
Formula biomarker concentration detection method is intervened for the variable in the present invention, the intervention variables A n is variation
Controllable, recordable current or voltage.
A kind of variable intervenes formula biomarker concentration detection device, including optical receiver module, transmission mechanism, adjusts dress
It puts and control system, the optical receiver module, regulating device is connect respectively with the transmission mechanism, the control system
System receives the electrical signal information of optical receiver module output and corresponding controling adjustment device movement.
Formula biomarker concentration detection device is intervened for the variable in the present invention, as transmission mechanism and tune
A kind of optional embodiment of regulating device, the transmission mechanism include intermeshing rack and pinion, adjusting dress
It puts including coil, magnet and pedestal, the rack is fixed on the optical receiver module, and the gear is rotatably solid
It is scheduled on the pedestal, the coil is fixed on the transmission mechanism, and the magnet is close to the coil so that
It can drive the light-receiving under the action of the magnet by the transmission mechanism after being powered into the coil
Block motion.
For in the present invention variable intervene formula biomarker concentration detection device, one as above-mentioned transmission mechanism
The further setting of kind embodiment, further includes axis, for the geared sleeve on the axis, the axis is fixed on pedestal
On.
Formula biomarker concentration detection device is intervened for the variable in the present invention, as transmission mechanism and tune
A kind of optional embodiment of regulating device, the transmission mechanism include intermeshing worm gear and worm screw, adjusting dress
It puts including coil, magnet and pedestal, the worm screw is fixed on the optical receiver module, and the worm gear is rotatably solid
It is scheduled on the pedestal, the coil is fixed on the transmission mechanism, and the magnet is close to the coil so that
It can drive the light-receiving under the action of the magnet by the transmission mechanism after being powered into the coil
Block motion.
Formula biomarker concentration detection device is intervened for above-mentioned variable in the present invention, as further setting
It puts, further includes spring, the optical receiver module is by the spring supporting.
Formula biomarker concentration detection device is intervened for above-mentioned variable in the present invention, as further setting
It puts, balancing device is additionally provided on the transmission mechanism, the balancing device is distributed in the transmission with the coil
The both sides of mechanism.
Formula biomarker concentration detection device is intervened for above-mentioned variable in the present invention, as magnet most
It is preferred that selecting, the magnet is neodium magnet.
The technique effect that the present invention is brought is：The present invention intervenes formula biomarker concentration detection method using variable and uses
Optical receiver module receives special spectrum information, forms spectrum, and set dead-center position, and determinand is adjusted in light by intervening variable
Spectral position on receiving module, the concentration value that the biomarker in determinand is calculated by intervention variable correspondence are believed
Breath can easily and fast, accurately detect the concentration of the biomarker in determinand；Variable in the present invention intervenes formula
Biomarker concentration detection device drives the adjustment of optical receiver module by recordable intervention Variable Control transmission mechanism,
So as to adjust the position of the spectrum on optical receiver module, and spectral position change information is obtained, when the electric signal that it is exported is
Initially to when can obtain the concentration of biomarker in detectable substance accordingly, simple in structure, cost of manufacture is cheap, and uses
Get up very convenient, precisely can quickly obtain the concentration of biomarker in detectable substance.
Description of the drawings
Fig. 1 be the present invention relates to surface plasma resonance technology fundamental diagram；
Fig. 2 is the work flow diagram of the variable intervention formula biomarker concentration detection method in invention；
Fig. 3 is intervention variables A n, the light-receiving mould of the variable intervention formula biomarker concentration detection method in the present invention
Correspondence mapping table between the electric signal output value Qn of block and biomarker concentration Bn；
Fig. 4 is the overall structure diagram of the variable intervention formula biomarker concentration detection device in the present invention.
Specific embodiment
Purpose, technical scheme and advantage to make the embodiment of the present invention are clearer, below in conjunction with the embodiment of the present invention
In attached drawing, the technical solution in the embodiment of the present invention is explicitly described, it is clear that described embodiment be the present invention
Part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
All other embodiments obtained under the premise of creative work are made, shall fall within the protection scope of the present invention.
Fig. 1 be the present invention relates to surface plasma resonance technology fundamental diagram, be the prior art, such as figure institute
Show, using the process of surface plasma resonance technology detection biomarker concentration, first, 300 He of flow channel is set
Sensing chip 400 sets one layer of golden film on sensing chip 400, and the one side part that sensing chip 400 is made to be equipped with golden film is located at
In flow channel 300 so that the object to be detected flowed into flow channel 300 can touch the sensing chip 400 with golden film,
During measurement, the object to be detected with biomarker is passed through flow channel 300 so that object to be detected is contacted with sensing chip,
One layer of antibody molecule is modified in reaction interface in advance, after target antigen and antibody identification, the refractive index of golden film surface dielectric
It changes, SPR resonance angles can change with the change of refractive index, the variation and the life with being incorporated in metal surface of refractive index
The variation of object macromolecular quality is directly proportional, and the size of formant displacement will reflect the change for being fixed on metal surface biotinylated molecular weight
Change, and then realize immunoassay.In Fig. 1, light beam is sent out by light source 600, be irradiated on sensing chip 400, after its reflection
It is received by optical detection unit 700 (will substitute optical detection unit 700 with optical receiver module in the present invention), passes through optical detection
Unit 700 senses the variation of the beam information received by it, obtains the change information and object biomarker concentration to be detected
Correspondence, biomarker concentration value in object to be detected is finally obtained from the change information.For light source 600, can adopt
It is preferable with monochromatic source, such as infrared beams light source, effect.
Variable in the present invention intervene the flow chart of formula biomarker concentration detection method as shown in Fig. 2, it include with
Lower processing step：S1, optical receiver module receive light beam and export electric signal, set it and export the zero Q0 of electric signal；S2、
It introduces and intervenes variables A n, each is made to intervene the electric signal output value Qn that variate-value An corresponds to an optical receiver module；S3, setting
Intervene the correspondence of variables A n and biomarker concentration Bn in detectable substance, and adjust and intervene variables A n to Am so that Qn=
Q0；S4, inquiry intervene the concentration Bm of the corresponding biomarkers of variables A m, that is, obtain the concentration of biomarker in detectable substance.
Above-mentioned detailed process is：Light beam is received using optical receiver module, spectrum can be formed above, and export electric signal Qn, if
Fixed its exports the zero Q0 of electric signal, which is optical receiver module when biomarker is not contained in the object to be detected being passed through
The value of electrical signals exported after light beam is received, variables A n is intervened in setting later, intervenes variable and is used for adjusting optical receiver module
The position of the spectrum of upper formation, while the electric signal output value of optical receiver module is adjusted, intervention variable herein can be changed
The variable quantity or adjustment light of the driving current of the incident angle for the light beam being irradiated on optical receiver module or voltage etc. connect
The variable quantity of the current or voltage of device of module position etc. is received, each intervenes variate-value An one optical receiver module of correspondence
Electric signal output value Qn；The correspondence of variables A n and biomarker concentration Bn in detectable substance are intervened in setting, are become due to intervening
Amount An is that the position for the spectrum that will be formed on optical receiver module is adjusted, at the slave sensing chip for causing detectable substance
The position of spectrum of the light beam of injection on optical receiver module and the detectable substance for not containing biomarker to be detected
The position overlapping of spectrum on optical receiver module, and pass through the value of electrical signals exported on optical receiver module and determine the specific of adjustment
Situation, when variables A n to Am is intervened in adjustment so that it is dense need to only to inquire the corresponding biomarkers of intervention variables A m at this time by Qn=Q0
Spend Bm, you can obtain the concentration of biomarker in detectable substance, above-mentioned n, m are natural number, and n>m.
It is of the invention further, intervene variables A n for the ease of adjusting, the intervention variables A n can be carried out specific
Refinement setting, such as cause An=A+n × a, wherein, A is definite value, can be zero or the current value of setting or voltage value etc., a
It (can be 0.01mA when for electric current that single to intervene variable, which adjusts amplitude,；Can be 0.01mV etc. when for voltage, it is all such
Class), the value of a is determined according to the required precision of detecting system, and when detecting system measurement accuracy is more demanding, a should be exhausted
The value that may be small and can realize, to meet the requirement of the measurement accuracy of measuring system, n is natural number.
Fig. 3 is intervention variables A n, the light-receiving mould of the variable intervention formula biomarker concentration detection method in the present invention
Correspondence mapping table between the electric signal output value Qn of block and biomarker concentration Bn, as shown in the figure, intervening variable
It is the concrete numerical value record after repeatedly adjusting in the list of An, the value of electrical signals Qn of optical receiver module output becomes to intervene variables A n
The concrete numerical value accordingly obtained with its variation after change, the numerical value of biomarker Bn corresponding with said two devices also can pre- antecedent
It is good, when measuring, An can be repeatedly adjusted, until the value of electrical signals Qm=Q0 of optical receiver module Qn outputs, is recorded corresponding
Intervene variables A m, you can the concentration Bm of (or calculating) corresponding biomarker is found from above-mentioned table.
In the present invention, intervene variate-value Am and gained after the zero Q0 of optical receiver module output electric signal, adjustment
The concentration value Bm of biomarker can be interval value, because for the content of the various composition in detectable substance, working as accuracy of detection
After reaching a certain level, the determinand in detectable substance will appear a degree of floating in flow process, in the present invention, treat
Object is surveyed in detection process, there are certain floatabilities with antigen binding due to antibody, this causes its light beam also to will appear
Micro variation, therefore the zero of the electric signal of optical receiver module output might have small-scale (such as the mathematical value of Q0 that floats
Section can be 0.0015-0.0016), it is therefore, related to this to intervene the dense of variate-value Am and corresponding biomarker
Angle value Bm will appear the interval value similar with Q0, and still, comparatively, there are the interval values and fixed-point values of slight change
When being mapped, the effect of the two is identical.
Intervene the above-mentioned intervention variable of formula biomarker concentration detection method for variable in the present invention, as long as can reach
It can record to micro be adjusted, light-receiving mould is realized in the change for example, by using recordable micro-current, trace voltage etc.
The adjusting of spectral position on block.
A kind of device that optical receiver module is adjusted using electric current is provided below, but it should be recognized that with following
For embodiment be based only on one kind of the above-mentioned principle of the present invention, other interchangeable schemes are more, and the present invention will no longer one by one
It enumerates.
Variable intervenes the overall structure diagram of formula biomarker concentration detection device as shown in figure 4, including light-receiving
Module 3, spring 4, gear 7, coil 9, magnet 10 and control system (not shown), one end of batten 2 is in articulated manner
Fixed, the other end is fixedly connected with rack 6, is set on the side of rack 6 there are one gear 7, gear 7 is in rack 6 and engages
State, gear 7 are rotatably fixed on axis 8, and axis 8 is fixed on stent 11, fix tooth on optical receiver module 3 for convenience
Optical receiver module 3 can be arranged on a batten 2 (but batten 2 can also save), then set rack on batten 2 by item 6
6.Coil 9 is fixed on gear 7, and magnet 10 is located at 9 side of coil, when being passed through electric current during control system is to coil 9, magnet 10
The magnetic field of generation can cause coil 9 to rotate, and so as to be moved with moving gear 7, adjust the position of optical receiver module 3, make above it
The position of spectrum changes.
As shown in figure 4, rack 6 is in meshing state with gear 7, and when gear 7 rotates under the effect of external force, rack 6
It is rotated with the rotation of gear 7, so as to which optical receiver module 3 be driven to move.In order to control/the rotation of adjustment gear 7,
Fixed on gear 7 one closure coil 9, which will extend the ontology of gear 7, and a distance that extends outwardly, with
One fixed magnet 10 of the side of coil 9 setting, a part for coil 9 is be overlapped with magnet 10, in this way since, when to coil 9
On when being passed through electric current, the coil 9 in closed state forms the circuit of a closure, when having electric current to lead in the circuit of this closure
Out-of-date, its own generates magnetic field under electromagnetic induction effect, and the magnetic field and the magnetic field of magnet 10 that coil 9 generates occur between each other
Active force, at this point, can to change magnetic field caused by coil 9 strong in control system change size of current/direction for being passed through in coil 9
Degree and magnetic direction, due to the size and Orientation in magnetic field caused by magnet 10 be it is fixed, when changing in coil 9
Size of current and/or during direction, under the action of the magnetic field of magnet 10, coil 9 rotates, since coil 9 is integrally fixed at
On gear 7, therefore, when coil 9 starts rotation, gear 7 is as coil 9 rotates and rotates, gear 7 and optical receiver module 3
On rack structure or rack 6 engage, therefore optical receiver module 3 moved with the rotation of gear 7 (in figure for vertical direction transport
It is dynamic).
As shown in figure 4, in order to further improve the adjusting sensitivity of the horizontal sextant angle of optical receiver module 3, in light-receiving mould
A spring 4 is connected on block 3, the other end of spring 4 is fixed, and sets an initial position so that spring 4 is in the initial position
Whole device is in a zero, can be by the elastic force of spring 4 when needing to adjust the horizontal sextant angle of optical receiver module 3 so that
Optical receiver module 3 is more prone to return back to initial position.Above-mentioned detailed process is shown as：When being passed through electric current, coil into coil 9
Middle generation magnetic field under the action of magnet 10, rotates, while gear 7 is also opened under the active force that coil 9 interacts in magnetic field around axis 8
Begin rotation, the rise/fall under the rotation of gear 7 of optical receiver module 3, while also begin to stretching/compressing spring 4, when light need to be made
When receiving module 3 returns back to the position of initial position or required setting, control system changes the electric current being passed through in coil 9 accordingly
Size or direction, for optical receiver module 3 in corresponding movement, spring 4 can provide convergent force or drawing force accordingly so that light
Receiving module 3 can it is sensitiveer under the assistance of the active force of spring 4, quickly return back to initial position or required position.
In Fig. 4, when setting batten 2, one end of batten 2 is hingedly fixed, is can be used herein including hinge, hinge
A variety of articulated mountings and hinged fixed form including receiving etc., i.e., need to only ensure that batten 2 can be rubbed around supporting point 1 with relatively low
Wipe power rotation.
For the shape of magnet 10 is in scheme, make its N pole and its S poles reasonable layout so that coil 9 can be in 10 magnetic of magnet
In the sphere of action of field, moved between the N poles of magnet 10 and S poles, it is sufficiently strong in order to generate for magnet 10
Magnetic field, it is preferred to use neodium magnet can obtain stronger magnetic field, can strength promote be powered after coil 9 movement.
In order to improve stability of the coil 9 on gear 7, and the adjusting sensitivity of coil 9 is further improved, in gear 7
On fix a balancing device 5, the balancing device 5 be mounted on gear 7 on, position is corresponding with coil 9, i.e., so that balance dress
Put 5 and coil 9 be located at the both sides of gear respectively, and the two is made to be located at same level height, plays certain put down each other
Weighing apparatus acts on, here, can balancing device 5 be arranged to coiled type, its part can be made to extend gear 7, so can be with
Mitigate the weight of balancing device 5, meanwhile, balancing device 5 can also be by its own gravity, to the position of coil 9 under leverage
It puts height and plays good balanced action, i.e., when coil 9 (is only referred to orientation, reality side in figure by the magnetic field force of magnet 10 to up/down
To without being limited thereto) movement when, under the action of balancing device 5,9 need of coil overcome the power of very little can break away from stationary state into
Row movement likewise, when that need to adjust coil 9 to initial position, only need to overcome smaller resistance can be realized, i.e., without big
The position adjustment to coil 9 can be realized in the electric current that amplitude variation is passed through in coil 9.
For the axis 8 in Fig. 3, it can be fixed by stent 11, in the present invention, the fixation end of batten 2, spring
4 fixing end and the bearing of stent 11 are the platform with supporting role, such as：Desk-top workbench or setting
Package unit however, it is also possible to add a rack or shell, is fixed on rack or outer by support platform in rack etc.
On shell.
For rotatable fixed form of the gear 7 on axis 8, the prior art can be used and solved, for example, can be used
Gear 7 is sleeved on the outer ring of bearing, makes it that can surround bearing and rotates, then the inner ring of bearing is fixed on axis 8, by axis 8
It is fixed on stent 11；In addition, Bearing gear also can be used directly in gear 7, it is directly anchored on axis 8；It is other existing
The mode of rotation connection, such as hinged, socket, etc. can realize that gear 7 is rotatably fixed and implement model in the present invention
Within enclosing.
It should be strongly noted that the variable in the present invention, which intervenes formula biomarker concentration detection device, implements not office
It is limited to above-mentioned embodiment, for example, wherein realize the gear 7 that optical receiver module 3 moves and the rack 6 engaged, it can
With by intermeshing turbine, worm screw or the nut of installation can be socketed, screw rod carries out whole replacement, and corresponding on demand will
The position of required change is modified, for example, worm screw or nut can be fixed on optical receiver module 3, by worm gear or spiral shell
Mother is fixed on pedestal, for other fields of those skilled in the art and the existing machinery field common knowledge of combination
Personnel very can readily make above-mentioned variation, no longer illustrate one by one here；Moreover, in the present invention, the position of spring 4 is also simultaneously
Position shown in figure is not limited to, can be also moved near supporting point 1, the function and effect of such spring 4 may be more
It is good.
The specific embodiment of the present invention is explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned
Embodiment, can also be under the premise of present inventive concept not be departed from the knowledge having in those skilled in the art
Various changes can be made.
Claims (4)
1. variable intervenes formula biomarker concentration detection method, it is characterised in that：Optical receiver module receives light beam, is formed
Spectrum, and electric signal Qn is exported, it sets it and exports the zero Q0 of electric signal；It introduces and intervenes variables A n, adjusted by intervening variable
The position of the spectrum formed on optical receiver module, while the electric signal output value of optical receiver module is adjusted, each intervenes variable
Value An corresponds to the electric signal output value Qn of an optical receiver module；Variables A n and biomarker concentration in detectable substance are intervened in setting
Variables A n to Am is intervened in the correspondence of Bn, adjustment so that the corresponding biomarker concentrations of variables A m are intervened in Qn=Q0, inquiry
Bm obtains the concentration of biomarker in detectable substance；
Above-mentioned, n, m are natural number, and n ＞ m.
2. variable according to claim 1 intervenes formula biomarker concentration detection method, it is characterised in that：Described is dry
Pre- variables A n=A+n × a, wherein, A is definite value, and a is the single adjustment amplitude for intervening variable, and n is natural number.
3. variable according to claim 1 intervenes formula biomarker concentration detection method, it is characterised in that：The light connects
The concentration value Bm for receiving the biomarker that module exports intervention variate-value Am and gained after the zero Q0 of electric signal, adjustment is equal
For interval value.
4. variable according to any one of claim 1-3 intervenes formula biomarker concentration detection method, feature exists
In：The intervention variables A n is controllable, the recordable current or voltage of variation.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131626.0A CN104749118B (en) | 2015-03-25 | 2015-03-25 | Variable intervenes formula biomarker concentration detection method and device |
| PCT/CN2015/077441 WO2016149980A1 (en) | 2015-03-25 | 2015-04-24 | Variable invasive biomarker concentration testing method and apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510131626.0A CN104749118B (en) | 2015-03-25 | 2015-03-25 | Variable intervenes formula biomarker concentration detection method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104749118A CN104749118A (en) | 2015-07-01 |
| CN104749118B true CN104749118B (en) | 2018-06-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510131626.0A Expired - Fee Related CN104749118B (en) | 2015-03-25 | 2015-03-25 | Variable intervenes formula biomarker concentration detection method and device |
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| Country | Link |
|---|---|
| CN (1) | CN104749118B (en) |
| WO (1) | WO2016149980A1 (en) |
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- 2015-04-24 WO PCT/CN2015/077441 patent/WO2016149980A1/en active Application Filing
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| EP1081529A2 (en) * | 1999-08-30 | 2001-03-07 | PSC Scanning, Inc. | Reflective dither spring |
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| CN103984091A (en) * | 2013-11-21 | 2014-08-13 | 苏州浩创信息科技有限公司 | Scanner oscillating mirror apparatus |
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| WO2016149980A1 (en) | 2016-09-29 |
| CN104749118A (en) | 2015-07-01 |
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