CN104777138B - Biological marker analyte detection light spectral position based on light reflection adjusts device - Google Patents

Biological marker analyte detection light spectral position based on light reflection adjusts device Download PDF

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Publication number
CN104777138B
CN104777138B CN201510131592.5A CN201510131592A CN104777138B CN 104777138 B CN104777138 B CN 104777138B CN 201510131592 A CN201510131592 A CN 201510131592A CN 104777138 B CN104777138 B CN 104777138B
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China
Prior art keywords
gear
batten
coil
fixed
analyte detection
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Expired - Fee Related
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CN201510131592.5A
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Chinese (zh)
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CN104777138A (en
Inventor
张贯京
陈兴明
葛新科
克里斯基捏·普拉纽克
艾琳娜·古列莎
王海荣
张少鹏
方静芳
高伟明
程金兢
梁艳妮
周荣
李慧玲
邢立立
波达别特·伊万
徐之艳
周亮
梁昊原
肖应芬
郑慧华
唐小浪
李潇云
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SHENZHEN SHENGBIZHI TECHNOLOGY DEVELOPMENT CO., LTD.
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Shenzhen Shengbizhi Technology Development Co Ltd
Shenzhen Beiwo Deke Biotechnology Research Institute Co Ltd
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Application filed by Shenzhen Shengbizhi Technology Development Co Ltd, Shenzhen Beiwo Deke Biotechnology Research Institute Co Ltd filed Critical Shenzhen Shengbizhi Technology Development Co Ltd
Priority to CN201510131592.5A priority Critical patent/CN104777138B/en
Priority to PCT/CN2015/077442 priority patent/WO2016149981A1/en
Publication of CN104777138A publication Critical patent/CN104777138A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55Specular reflectivity
    • G01N21/552Attenuated total reflection

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Biological marker analyte detection light spectral position based on light reflection adjusts device.The present invention relates to biomarker detection fields, more specifically to it is used for the auxiliary device of biological marker analyte detection, including batten (2), mirror (3), gear (7), axis (8), coil (9) and magnet (10), mirror (3) is arranged on batten (2), batten (2) one end is hingedly fixed, the rack structure engaged with gear (7) is offered on batten (2), gear (7) is rotatably fixed on axis (8), coil (9) is fixed on gear (7), magnet (10) is close to coil (9), enable after being powered into coil (9) it in the effect of magnet (10) around axis (8) rotary motion.The spectral position that the infrared radiation reflection light of biomarker in detectable substance in biomarker surface plasma resonance technology checking process can easily and efficiently be changed, to obtain the effective information of biomarker in detectable substance.

Description

Biological marker analyte detection light spectral position based on light reflection adjusts device
Technical field
The present invention relates to biomarker detection fields, more specifically to the biological marker analyte detection based on light reflection Device is adjusted with spectral position.
Background technique
Biomarker (Biomarker) refers to can be with tagging system, organ, tissue, cell and subcellular structure or function The biochemical indicator of change or the change that may occur of energy, has very extensive purposes.Biomarker can be used for disease and examine Break, judge staging or for evaluating the safety and efficacy of new drug or new treatment in target group.It is given birth in detection When object marker, surface plasma resonance technology SPR (Surface Plasmon Resonance) is commonly used, that is, utilizes metal The total reflection of film/liquid level interface light connects a kind of caused physical optics phenomenon to analyze bio-molecular interaction, and passes through Infrared light combination SPR technique measures biomarker concentration, and specifically can refer to paper, " surface plasma resonance technology is cured in biology Application in " -2004 grades of Peking University's biomedical engineering Zhang Rui Bin (10403048) and paper " surface plasma Application and its progress of the resonance immunosensor in protein detection "-" analytical chemistry " the 7th phase 1052- in 2010 1059, however, how can more accurately obtain the characteristic spectrum of the infrared light of detection biomarker in measurement process Location information is pendulum in face of numerous field of biomedicine scholars to obtain the relevant information of biomarker in determinand A difficult problem.
Summary of the invention
In order to solve the above technical problem, the present invention provides one kind can obtain the reflection light of biomarker, and adjusts The biological marker analyte detection light spectral position based on light reflection of whole biomarker reflected light spectral position adjusts device.
The present invention to solve above-mentioned technical problem used by technical solution are as follows: based on light reflection biological marker analyte detection Adjust device with spectral position, including batten, mirror, gear, axis, coil and magnet, the mirror setting on batten, Described batten one end is hingedly fixed, and the rack structure engaged with the gear, the gear are offered on the batten Rotatably be fixed on axis, the coil is fixed on the gear, the magnet close to the coil so as to In the coil be powered after its can the magnet effect around the axis rotary motion.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as further Setting, the coil extends the gear.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as further Setting, be additionally provided with balancing device on the gear, and the balancing device and the coil are distributed in the tooth The two sides of wheel.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, is filled as balance A kind of preferred embodiment set, the balancing device are the annulus of closure.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as item Plate advanced optimizes, and the batten includes straightway and bending segment, and the mirror is fixed on the straightway, institute The bending segment stated includes horizontal segment and vertical section, and the rack structure is arranged in the vertical section.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as further Setting, the mirror along the straightway of the batten extending direction be distributed.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as further Setting, the invention also includes spring, the batten is by the spring supporting.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, it is solid as spring The further setting set is positioned, the spring one end is fixed, and the other end is connect with the horizontal segment of the bending segment of the batten, But simultaneously, another position can also be arranged in spring, and the specially spring one end is fixed, the other end and the batten Hinged fixing end connection.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as magnetic The optimum alternative of iron, the magnet select the neodium magnet of high magnetism, it is ensured that coil after powered up can be by magnet Biggish magnetic field force encloses pivoting so as to band moving gear together.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as bullet Spring fixes the design scheme of batten Support Position, the spring one end, and the hinged fixing end of the other end and the batten connects It connects, i.e., the position provided spring from attached drawing is moved to close to the hinged end of batten.
Device is adjusted for the biological marker analyte detection light spectral position based on light reflection in the present invention, as further Setting, the axis is fixed on bracket.
In the present invention, it can cover on the outer ring of bearing, then bearing is fixed on axis, so that gear by what gear was fixed Pivoting movement can be enclosed under lower resistance;But Bearing gear can also be directly selected when application selects gear, directly by it It is fixed on axis.Meanwhile for this problem, existing other way also can be used, will not enumerate herein.
In the present invention, for the engagement system of gear and rack gear, similar engagement system can also be selected to be replaced, example Such as: gear is changed to worm gear, coil is fixed on worm gear, the rack structure on batten is replaced with into worm screw or worm structure, It is transmitted by the engagement of worm and gear, completes the adjusting of mirror to batten and its above;Gear can also be replaced with to nut, phase That answers is fixed on coil on nut, the rack structure on batten is changed into threaded rod or similar structures, so that coil is in magnet Under the action of by driving nut rotation, and then threaded rod, batten is driven to be moved, mirror so as to adjust batten and its above The position of son or/and angle.
The present invention is based on the biological marker analyte detection light spectral positions of light reflection to adjust device by adopting the above technical scheme, band The technical effect come are as follows: be fixed since present invention employs the battens for being fixed with mirror, and by batten one end, the other end By spring supporting, rack structure is provided on batten, and engage it with gear, on gear near fixed coil Equipped with magnet, electric current (or change and be passed through the size and Orientation of electric current in coil) is being passed through into coil, due to electromagnetic induction original Reason, coil generate magnetic field, and the magnetic field interaction that the magnetic field and magnet generate, coil band moving gear turns around axis in this role It is dynamic, and then batten is driven to be moved, change the angle of the mirror on batten, thus change incidence/shooting angle of infrared ray, The position that related infrared light is formed by dark space on spectrum acquisition device is controlled, the biomarker in detectable substance is obtained Relevant information, the adjustment process is very succinct reliable, and is very easy to operation, and high sensitivity can easily and efficiently change life In object marker surface plasma resonance technology checking process in detectable substance the infrared radiation reflection light of biomarker light Spectral position information, to obtain the effective information of biomarker in detectable substance.
Detailed description of the invention
Fig. 1 is the overall structure signal that the biological marker analyte detection light spectral position reflected the present invention is based on light adjusts device Figure;
Fig. 2 is the use state signal that the biological marker analyte detection light spectral position reflected the present invention is based on light adjusts device Figure.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention In attached drawing, technical solution in the embodiment of the present invention is explicitly described, it is clear that described embodiment is the present invention A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
Fig. 1 is overall structure diagram of the invention, as shown, the biological marker analyte detection based on light reflection uses up spectrum Position regulator, including batten 2, spring 4, gear 7, coil 9 and magnet 10, one end of batten 2 are fixed in articulated manner, The other end is fixedly connected with rack gear 6, and the side of rack gear 6 is provided with a gear 7, and gear 7 and rack gear 6 are in meshing state, Gear 7 is rotatably fixed on axis 8, and axis 8 is fixed on bracket 11, for convenience the stationary rack 6 on batten 2, can be by item Plate 2 is set as the two parts being fixedly connected, and a part is straightway 21, and another part is bending segment 22, one end of straightway 21 It is fixed by the way of hinged, the other end is fixedly connected with bending segment 22, and makes straightway 21 and horizontal direction in certain Angle (in the majority with acute angle under normal circumstances), mirror 3 is fixed on straightway 21, for reflect detection biomarker when Infrared light, bending segment 22 is in 90 degree of bending-likes, and horizontal component is fixedly connected with straightway, vertical portion stationary rack 6, it is of course also possible to rack structure is arranged in the vertical section, rack gear is arranged with bending segment 22 it is into a single integrated structure, compare and Speech, is arranged rack structure in the vertical portion of bending segment 22, that is, is made of one the structure of formula in production, installation and use It can be more convenient.
Rack structure or fixed rack gear 6 and gear 7 on batten 2 are in meshing state, when gear 7 is in the work of external force When with lower rotation, rack structure or rack gear 6 on batten 2 are rotated with the rotation of gear 7, so that batten 2 be driven to transport It is dynamic.In order to control/the rotation of adjustment gear 7, the coil 9 of a closure is fixed on gear 7, which will extend gear 7 Ontology, and a distance that extends outwardly, with coil 9 beside a fixed magnet 10 is set, a part of coil 9 with Magnet 10 is overlapped, in this way since, when being passed through electric current on coil 9, coil 9 in closed state forms time an of closure Road, when having electric current to pass through in the circuit of this closure, its own generates magnetic field under electromagnetic induction effect, what coil 9 generated The magnetic field of magnetic field and magnet 10 is had an effect power between each other, at this point, changing size of current/direction for being passed through in coil 9 can be with Change magnetic field strength and magnetic direction caused by coil 9, the size and Orientation in the magnetic field as caused by magnet 10 is solid Fixed, therefore, when changing size of current and/or the direction in coil 9, under the action of the magnetic field of magnet 10, coil 9 occurs Rotation, since coil 9 is fixed on gear 7, when coil 9 starts turning, gear 7 turns as coil 9 rotates It is dynamic, gear 7 on batten 2 rack structure or rack gear 6 engage, therefore rack structure on batten 2 or rack gear 6 are with gear 7 Rotation and move (in figure move for vertical direction), final batten 2 can also be moved with the variation of electric current in coil 9, right In batten 2, due to its straightway 21 be it is fixed in articulated manner, in batten 2 with being passed through electric current in coil 9 Variation and when changing, the straightway 21 of batten 2 and the angle of horizontal direction will also change therewith, in turn, be fixed on batten 2 On mirror 3 and horizontal direction angle also just with change, the angle of reflection light above can also change therewith.
As shown in Figure 1, in order to further increase the adjusting sensitivity of the horizontal sextant angle of the mirror 3 on batten 2, in batten 2 One spring 4 of upper connection, the other end of spring 4 are fixed, and an initial position are set, so that spring 4 is whole in the initial position A device is in a zero point, can be by the elastic force of spring 4, so that mirror 3 is more when needing to adjust the horizontal sextant angle of mirror 3 Add and is easy to return back to initial position.Above-mentioned detailed process is showed themselves in that when being passed through electric current into coil 9, generates magnetic field in coil, Under the action of magnet 10, coil 9 is rotated under the active force of magnetic field interaction around axis 8, while gear 7 also starts turning, on batten 2 Rack structure or fixed installation rack gear 6 under the rotation of gear 7 rise/fall, and then drive batten 2 rise overally/under Drop also starts stretching/compressing spring 4 simultaneously when batten 2 begins to ramp up/declines, when need to make batten 2 and its mirror above When son 3 returns back to the position of initial position or required setting, when changing size of current or the direction being passed through in coil 9 accordingly, Batten 2 with above it rack structure or rack gear 6 accordingly move when, spring 4 can provide convergent force or drawing accordingly Stretch, such that batten 2 is sensitiveer under the assistance of the active force of spring 4, quickly returns back to initial position or required Position.
Setting for 4 position of spring, can refer to Fig. 1, will be one end of which is fixed on bending segment 22, and the other end is fixed on phase The position (such as in platform or rack etc.) answered.
In Fig. 1, one end of batten 2 be it is hinged fixed, can be used herein more including hinge, hinged ball etc. Kind articulated mounting and hinged fixed form need to only ensure that batten 2 can be around supporting point 1 with the rotation of lower frictional force.
As shown in fig. 1, for the shape of magnet 10 is in scheme, in vertical direction, (this direction is intuitive direction in figure, tool The setting orientation of body material object is not limited thereto) setting magnet 10, and make its pole N and its pole S reasonable layout, enables coil 9 It is enough to be moved between the pole N and the pole S of magnet 10 in the sphere of action in 10 magnetic field of magnet, for magnet 10, in order to can Generate sufficiently strong magnetic field, it is preferred to use neodium magnet can obtain stronger magnetic field, can strength promote be powered after The movement of coil 9.
Further, as shown in fig. 1, in order to improve stability of the coil 9 on gear 7, and coil 9 is further increased Adjusting sensitivity, a balancing device 5 is fixed on gear 7, which is mounted on gear 7, and position is wanted and line Circle 9 is corresponding, i.e., so that balancing device 5 and coil 9 are located at the two sides of gear, and the two is made to be located at same level height, Certain balanced action is played each other, here, balancing device 5 can be arranged to coiled type, its part can be made to extend Gear 7 out can so mitigate the weight of balancing device 5, meanwhile, balancing device 5 can also be by self gravity, in thick stick Good balanced action played to the position height of coil 9 under bar effect, i.e., when coil 9 by magnet 10 magnetic field force to up/down When (only referring to orientation in figure, actual direction is without being limited thereto) moves, under the action of balancing device 5,9 need of coil overcome very little Power can get rid of stationary state and be moved, likewise, need to only overcome lesser when that need to adjust coil 9 to initial position Resistance can be realized, i.e., the position adjustment to coil 9 can be realized without the electric current that significantly variation is passed through in coil 9.
For the axis 8 in Fig. 1, it can be fixed by bracket 11, in embodiment given by Fig. 1 of the present invention, item The bearing of the fixation end of plate 2, the fixing end of spring 4 and bracket 11 is the platform with supporting role, such as: it is desk-top The support platform that is perhaps arranged on the rack of workbench etc. however, it is also possible to add a rack or shell, will be a whole set of Device is fixed on rack or shell.
In the present invention, for rotatable fixed form of the gear 7 on axis 8, the prior art can be used and solved, example Such as, it can be used 7 sets of gear on the outer ring of bearing, rotate it can around bearing, the inner ring of bearing is then fixed on axis 8 On, axis 8 is fixed on bracket 11;In addition, Bearing gear also can be used directly in gear 7, it is directly anchored on axis 8;Its The mode of its existing rotation connection, it is rotatably fixed of the invention that such as hinged, socket, etc. can be realized gear 7 Within enforceable range.
Fig. 2 is the use state signal that the biological marker analyte detection light spectral position reflected the present invention is based on light adjusts device Figure as shown in the figure when in use, can be first using the detection liquid without biomarker, using infrared light to detection device It is irradiated, and is irradiated to its reflected light on mirror 3 by direction shown in Fig. 2, after the reflection of mirror 3, enter spectrum Reception device 12 receives the infrared ray after the reflection of mirror 3 by spectrum acquisition device 12, and determines the position of spectrum dark space, In such cases, device is adjusted with light spectral position the present invention is based on the biological marker analyte detection of light reflection be set as initial position, Correspondingly, the dark space being formed by band-like smooth area and band-like smooth area at spectrum acquisition device 12 under the state is also made accordingly It is anti-in detection device due to the presence of biomarker in detectable substance when changing measured detectable substance for initial position Body absorbs antigen, and the reflected light for detecting the infrared light of irradiation changes, that is, the infrared light being irradiated on mirror 3 of the present invention It changes, and then after its reflection, infrared light received by spectrum acquisition device 12 sends variation, fills in spectrum acquisition Set the spectrum formed on 12 and also send variation therewith, be formed by the position of dark space also will relative initial position occur it is inclined It moves.
It take incident ray therein as the infrared external reflection light of detectable substance detected, using its emergent light as light referring to Fig. 2 The light for composing 12 actual received of reception device, when need to be adjusted to the dark space position of the spectrum formed on spectrum acquisition device 12 It is whole, when further relating to the details of biomarker to obtain, change size of current or/and the direction being passed through in coil 9, The intensity in magnetic field caused by the coil 9 of closure or/and direction change also with sending, in this way since, in the effect of magnet 10 Under, coil 9 rotates with moving gear 7 and (is easier the magnetic force in magnet 10 in the balancing brake effect lower coil 9 of balancing device 5 The lower movement of effect), and then gear 7 drives rack structure or rack gear 6 on batten 2 to move, the also setting in motion of batten 2, mirror 3 with The angle of horizontal direction changes with the movement of batten 2, so that the incidence angle for the incident light being irradiated on mirror 3 It changes, correspondingly, the angle of emergence also changes, the angle that emergent light is irradiated on spectrum acquisition device 12 also becomes Change, therefore the position for the dark space that the infrared ray of object reflection is formed on spectrum acquisition device 12 after testing can also change, The size of electric current or/and direction in coil 9 are passed through according to adjustment, it is corresponding to control the dark space formed on spectrum acquisition device 12 Position, the metrical information needed for being obtained by the change of the dark space position and the change for the electric current being passed through in coil 9.
It should be strongly noted that implementation of the invention is not limited to above-mentioned embodiment, for example, wherein realizing item Rack structure or rack gear 6 on the gear 7 of the movement of plate 2 and the batten 2 engaged, can be by intermeshing turbine, snail Bar can be socketed the nut of installation, the whole replacement of screw rod progress, and the corresponding position as needed by required change is repaired Change, for those skilled in the art and combines other field personnel of existing machinery field common knowledge can be very Readily make above-mentioned variation;Moreover, the position of spring 4 is also not limited to position shown in figure in the present invention, can also incite somebody to action It is moved near supporting point 1, and the function and effect of such spring 4 may be more preferable.
Above in conjunction with attached drawing, the embodiment of the present invention is explained in detail, but the present invention is not limited to above-mentioned Embodiment within the knowledge of those skilled in the art can also be without departing from the purpose of the present invention Various changes can be made.

Claims (5)

1. the biological marker analyte detection light spectral position based on light reflection adjusts device, it is characterised in that: including batten (2), mirror Sub (3), gear (7), axis (8), coil (9) and magnet (10), the mirror (3) are arranged on batten (2), the item Plate (2) one end is hingedly fixed, and the rack structure engaged with the gear (7), the batten are offered on the batten (2) It (2) include straightway (21) and bending segment (22), the mirror (3) is fixed on the straightway (21), and described is curved Trisection (22) includes horizontal segment and vertical section, and the rack structure is arranged in the vertical section of the bending segment (22), described Gear (7) be rotatably fixed on axis (8), the coil (9) is fixed on the gear (7), the coil (9) extend described gear (7) ontology, the magnet (10) is close to the coil (9), so as in the coil (9) It can be additionally provided on the gear (7) under the action of the magnet (10) around the axis (8) rotary motion after energization Balancing device (5), the balancing device (5) and the coil (9) are distributed in the two sides of the gear (7), and described is flat Weighing apparatus device (5) is the annulus of closure.
2. the biological marker analyte detection light spectral position according to claim 1 based on light reflection adjusts device, feature Be: the mirror (3) is distributed along the extending direction of the straightway (21) of the batten (2).
3. the biological marker analyte detection light spectral position according to claim 2 based on light reflection adjusts device, feature It is: further includes spring (4), the batten (2) is supported by the spring (4).
4. the biological marker analyte detection light spectral position according to claim 3 based on light reflection adjusts device, feature Be: described spring (4) one end is fixed, and the other end is connect with the horizontal segment of the bending segment (22) of the batten (2).
5. the biological marker analyte detection light spectral position according to claim 1 based on light reflection adjusts device, feature Be: the axis (8) is fixed on bracket (11).
CN201510131592.5A 2015-03-25 2015-03-25 Biological marker analyte detection light spectral position based on light reflection adjusts device Expired - Fee Related CN104777138B (en)

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Application Number Priority Date Filing Date Title
CN201510131592.5A CN104777138B (en) 2015-03-25 2015-03-25 Biological marker analyte detection light spectral position based on light reflection adjusts device
PCT/CN2015/077442 WO2016149981A1 (en) 2015-03-25 2015-04-24 Light reflection-based spectral position adjusting apparatus for biomarker testing

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Application Number Priority Date Filing Date Title
CN201510131592.5A CN104777138B (en) 2015-03-25 2015-03-25 Biological marker analyte detection light spectral position based on light reflection adjusts device

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CN104777138B true CN104777138B (en) 2019-05-17

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CN114166806B (en) * 2021-11-13 2023-06-27 安徽工程大学 Ultraviolet lamp interaction experiment table for performing fluorescence analysis on biological sample

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EP0730241A2 (en) * 1990-05-08 1996-09-04 Symbol Technologies, Inc. Scanning arrangement
EP1081529A2 (en) * 1999-08-30 2001-03-07 PSC Scanning, Inc. Reflective dither spring
CN201075777Y (en) * 2007-08-31 2008-06-18 上海世科嘉车辆技术研发有限公司 Electric angle regulating system of rear view mirror single electric machine
CN201212871Y (en) * 2008-04-30 2009-03-25 中国科学院金属研究所 Test system for dynamic bending fatigue performance of thin-film material
CN102967934A (en) * 2012-12-04 2013-03-13 中国科学院苏州纳米技术与纳米仿生研究所 Electromagnetic-driven micro mirror
CN103984091A (en) * 2013-11-21 2014-08-13 苏州浩创信息科技有限公司 Scanner oscillating mirror apparatus
CN204536183U (en) * 2015-03-25 2015-08-05 深圳市易特科信息技术有限公司 For the light reflective spectrum position regulator that biomarker detects

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