CN104740233A - Chinese medicinal composition and preparation method thereof - Google Patents
Chinese medicinal composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal composition, which is prepared by grinding the following crude drugs by weight into fine powder: 50-60 parts of a silky fowl extract, 100-110 parts of deer-horn glue, 50-60 parts of vinegar processed turtle, 30-50 parts of calcined oyster, 50-60 parts of Mantis Egg-case, 100-110 parts of ginseng, 20-30 parts of astragalus, 120-130 parts of Chinese angelica, 100-110 parts of radix paeoniae alba, 100-110 parts of vinegar processed cyperus rotundus, 50-60 parts of radix asparagi, 20-30 parts of licorice, 200-220 parts of rehmannia root, 200-220 parts of prepared rehmannia root, 50-60 parts of Ligusticum wallichii, 20-30 parts of Radix Stellariae, 100-110 parts of Salvia Miltiorrhiza, 100-110 parts of Chinese yam, 50-60 fried Euryale ferox, and 30-50 parts of Cornu Cervi Degelatinatum. The composition has anti-inflammatory and antispasmodic effects.
Description
Technical field
The invention belongs to field of medicaments, especially relate to a kind of Chinese medicine composition and preparation method thereof.
Background technology
Wuji Baifen pian originates from Imperial Medical Service of the Ming Dynasty and compiles " Prescriptions for Universal Relief ", and Qing Dynasty imperial palace imperial physician is improved to Wuji Baifen pian, and modern age is named mainly with Wuji Baifen pian.Wuji Baifen pian prescription composition changes greatly, and has multiple prescription, colleague's Wuji Baifen pian that the contained Wuji Baifen pian of " Chinese Pharmacopoeia " 2010 editions, ministry standard are contained, 12 Wuji Baifen pians, Wuji Baifen pian, WUJI BAIFENG capsule etc.Wuji Baifen pian is made up of two Ten Herbs such as Gallus Domesticus, Radix Ginseng, the Radix Paeoniae Alba, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperis, with blackbone Chicken benefiting QI and nourishing blood, and principal agent in the enriching yin and nourishing kidney side of being; Compatibility Radix Ginseng, the Radix Astragali, Rhizoma Dioscoreae, QI invigorating; Radix Angelicae Sinensis, Radix Rehmanniae Preparata, Rhizoma Chuanxiong, the Radix Paeoniae Alba, Radix Salviae Miltiorrhizae, nourshing blood and promoting blood circulation; Colla cornus cervi, temperature male wind-supplying kidney, beneficial intensive culture blood; Other first, nourishing YIN for suppressing the hyperactive YANG, the Radix Rehmanniae, Radix Asparagi, the moon of liver and kidney tonifying; Dissolving lump and resolving mass; Rhizoma Cyperi, depressed liver-energy dispersing and QI regulating, menstruction regulating and pain relieving; Ootheca Mantidis, Concha Ostreae (calcined), Cornu Cervi Degelatinatum, Semen Euryales, restrain astringent or styptic treatment for spontaneous sweating; Radix Stellariae, clearind deficient heat; Radix Glycyrrhizae, coordinating the actions of various ingredients in a prescription has effect of benefiting qi and nourishing blood, adjusting meridian and stopping leukorrhea, cures mainly the asthenic body that deficiency of both QI and blood causes, waist soreness, night sweat due to yin deficiency, irregular menstruation, uterus deficiency and coldness, menalgia, bleeding not during menses, the disease such as to lose blood puerperal.Research shows, Wuji Baifen pian can reduce rat triglyceride and OxLDL ELISA, prevention arteriosclerosis has control sclerotin loose, and the effects such as the liver protecting, have potential medical value to Alzheimer.More than CP method 20 tastes, Radix Rehmanniae Preparata, Radix Rehmanniae, Rhizoma Chuanxiong, Cornu Cervi Degelatinatum, Radix Stellariae, Semen Euryales, Rhizoma Dioscoreae, Radix Salviae Miltiorrhizae eight taste are ground into coarse powder, 12 tastes such as all the other Gallus Domesticuss, point
do not drinkgive cataclasm, put in tank, separately add yellow wine 1500g, add a cover closed, stewing out of water is most to wine, takes out, mixes even, low temperature with above-mentioned coarse powder
dry, then be ground into fine powder, and sieve, mixing.Every 100g powder adds refined honey 30 ~ 40g and appropriate water, general ball, dry, makes water-honeyed pill; Or add refined honey 90 ~ 120g and make small honey pill or big honeyed pills, to obtain final product.In preparation method, impurity is many, affects the treatment.
Patent CN03130018.9 preparation method is too loaded down with trivial details, is not suitable for industrialization and produces.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of Chinese medicine composition, said composition has the spasmolytic effect that eases pain very well.
Present invention also offers the preparation method of said composition, the method is applicable to industrialization and produces.
The present invention is achieved by the following technical solutions:
A kind of Chinese medicine composition, it is characterized in that, be prepare by the following method:
(1) weight portion medical material is got: Gallus Domesticus extract 50-60 part, Colla cornus cervi 100-110 part, Carapax Trionycis (processed with vinegar) 50-60 part, Concha Ostreae (calcined) 30-50 part, Ootheca Mantidis 50-60 part, Radix Ginseng 100-110 part, Radix Astragali 20-30 part, Radix Angelicae Sinensis 120-130 part, Radix Paeoniae Alba 100-110 part, Rhizoma Cyperi (processed with vinegar) 100-110 part, Radix Asparagi 50-60 part, Radix Glycyrrhizae 20-30 part, Radix Rehmanniae 200-220 part, Radix Rehmanniae Preparata 200-220 part, Rhizoma Chuanxiong 50-60 part, Radix Stellariae 20-30 part, Radix Salviae Miltiorrhizae 100-110 part, Rhizoma Dioscoreae 100-110 part, Semen Euryales fry 50-60 part, Cornu Cervi Degelatinatum 30-50 part, are ground into fine powder for subsequent use;
(2) get the Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae ethanol extraction, filtrate crosses macroporous resin, ion, then uses ethanol elution, and collect eluent, drying under reduced pressure, obtains extract 1
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi ethanol extraction, obtain extract 2;
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum water extract-alcohol precipitation, supernatant concentrating under reduced pressure is dry, obtains extract 3;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
In above-mentioned steps (1) preferred Chinese medicine composition be fry 54 parts by weight portion Gallus Domesticus extract 40 parts, Colla cornus cervi 108 parts, Carapax Trionycis (processed with vinegar) 54 parts, Concha Ostreae (calcined) 40 parts, Ootheca Mantidis 40 parts, Radix Ginseng 108 parts, the Radix Astragali 27 parts, Radix Angelicae Sinensis 125 parts, the Radix Paeoniae Alba 108 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Asparagi 54 parts, 27 parts, Radix Glycyrrhizae, Radix Rehmanniae 215 parts, Radix Rehmanniae Preparata 216 parts, Rhizoma Chuanxiong 54 parts, Radix Stellariae 20 parts, Radix Salviae Miltiorrhizae 108 parts, Rhizoma Dioscoreae 108 parts, Semen Euryales, Cornu Cervi Degelatinatum 40 parts makes.
In described step (1), Gallus Domesticus extract is obtained by following method: the Gallus Domesticus of getting removing feather claw and intestine is cut into block, add 2-5 times of water gaging, boil 0.5-4 hour, use 5-5: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, adjust pH, to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boil 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use.
The Radix Astragali in described step (2), the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, the Radix Glycyrrhizae 60-100% ethanol extraction of 4-6 times of medical material amount, extract 1-3 time, each 2-4 hour, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column, first wash with water, then use 50-95% ethanol elution, collect eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
Described resin column model includes but not limited to AB-8 post, D101 post, D201 post, D301 post, HPD100 post, HPD300 post, HPD600 post, NKA-9 post, DA201 type, D-type, SIP series, X-5 type, GDX104 type, LD605 type, LD601 type, CAD-40 type, DM-130 type, R-A type, CHA-111 type, WLD type (mixed type), H107 type etc.
Can also adsorb with polyamide column, silicagel column, aluminium oxide etc. in step of the present invention (2).
In preferably described step (2), add macroporous resin column or polyamide column, first wash with water, use 50,70%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 70%, 80%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.Adopt gradient elution by active substance eluting completely, waste can be avoided.
Rhizoma Chuanxiong in described step (3), Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 70-95% ethanol extraction 1-3 time, each 2-4 hour, extracting solution merges, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 4-6 times soak by water in described step (4), boiling water decocts 2-4 hour, filter, merge decoction liquor, decoction liquor stand at low temperature, with filter plates, it is 70-85% that filtrate is sink to determining alcohol with 60-95% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is.Stand at low temperature can by water-soluble substances protein, and the precipitation such as tannin impurity is removed, time saving and energy saving, can save the life-span of filter plate.
Present invention also offers the preparation method of Chinese medicine composition, comprise the steps:
(1) weight portion medical material is got: Gallus Domesticus extract 50-60 part, Colla cornus cervi 100-110 part, Carapax Trionycis (processed with vinegar) 50-60 part, Concha Ostreae (calcined) 30-50 part, Ootheca Mantidis 50-60 part, Radix Ginseng 100-110 part, Radix Astragali 20-30 part, Radix Angelicae Sinensis 120-130 part, Radix Paeoniae Alba 100-110 part, Rhizoma Cyperi (processed with vinegar) 100-110 part, Radix Asparagi 50-60 part, Radix Glycyrrhizae 20-30 part, Radix Rehmanniae 200-220 part, Radix Rehmanniae Preparata 200-220 part, Rhizoma Chuanxiong 50-60 part, Radix Stellariae 20-30 part, Radix Salviae Miltiorrhizae 100-110 part, Rhizoma Dioscoreae 100-110 part, Semen Euryales fry 50-60 part, Cornu Cervi Degelatinatum 30-50 part, are ground into fine powder for subsequent use;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, the Radix Glycyrrhizae 60-100% ethanol extraction of 4-6 times of medical material amount, extract 1-3 time, each 2-4 hour, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column, first wash with water, then use 50-95% ethanol elution, collect eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1;
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 70-95% ethanol extraction 1-3 time, each 2-4 hour, extracting solution merges, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 4-6 times of soak by water, boiling water decocts 2-4 hour, filter, merge decoction liquor, decoction liquor stand at low temperature, with filter plates, it is 70-85% that filtrate is sink to determining alcohol with 60-95% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
The preparation method of preferred Chinese medicine composition, comprises the steps:
(1) weight portion medical material is got: Gallus Domesticus extract 40 parts, Colla cornus cervi 108 parts, Carapax Trionycis (processed with vinegar) 54 parts, Concha Ostreae (calcined) 40 parts, Ootheca Mantidis 40 parts, Radix Ginseng 108 parts, the Radix Astragali 27 parts, Radix Angelicae Sinensis 125 parts, the Radix Paeoniae Alba 108 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Asparagi 54 parts, 27 parts, Radix Glycyrrhizae, Radix Rehmanniae 215 parts, Radix Rehmanniae Preparata 216 parts, Rhizoma Chuanxiong 54 parts, Radix Stellariae 20 parts, Radix Salviae Miltiorrhizae 108 parts, Rhizoma Dioscoreae 108 parts, Semen Euryales fry 54 parts, Cornu Cervi Degelatinatum 40 parts is for subsequent use;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 95% ethanol extraction of 5 times of medical material amounts, extract 2 times, each 3 hours, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column or polyamide column, first wash with water, use 50,70%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 70%, 870%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1;
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 95% ethanol extraction 2 times, each 3 hours, extracting solution merged, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2;
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 5 times of soak by water, boiling water decocts 4 hours, filter, merge decoction liquor, decoction liquor 0-4 DEG C of stand at low temperature, with filter plates, it is 70% that filtrate is sink to determining alcohol with 85% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
The present invention also provides the pharmaceutical preparation be prepared into as active constituents of medicine with Chinese medicine composition of the present invention, pharmaceutical preparation of the present invention, and comprising compositions is by weight percentage 0.1-99.9%, and all the other are pharmaceutically acceptable carrier.The weight ratio of preferred Chinese medicine composition extract powder of the present invention and adjuvant is 3: 1-1: 3, and the best is extract powder is 1: 1 with other supplementary product consumption ratios.
Pharmaceutical preparation of the present invention can be any pharmaceutically useful dosage form, conventionally prepare, these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably peroral dosage form, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc., be more preferably capsule, granule, tablet and oral liquid.
Carrier of the present invention is that the carrier pharmaceutically commonly used is selected from mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its derivates, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
All according to weight portion as proportioning in above-mentioned composition of the present invention, aborning can according to corresponding proportion increase or reduce, as large-scale production can by kilogram or ton in units of, small-scale production can be counted in units of mg.Weight can increase or reduce, but the raw medicinal herbs weight proportion ratio between each composition can not change.
Above part by weight of the present invention is through that science filters out, can corresponding increase or reduce the corresponding ratio of adjustment for especial patient such as serious symptom or mild, but increase and decrease or reduce and be no more than 300%, and effect is constant.
Single medical material in above Chinese medicine composition, especially ministerial drug and adjuvant drug can be replaced by the Chinese medicine suitably with the identical property of medicine, and after replacing, the drug effect of Chinese medicine preparation is constant.
The present invention other optimal way in a particular embodiment.
Chinese medicine composition of the present invention and preparation thereof have the effect having antiinflammatory and spasmolytic.
In order to confirm the above-mentioned beneficial effect of technical solution of the present invention, inventor has done the pharmacodynamic feature of corresponding pharmacodynamics test and contrast test many-side research medicine of the present invention.
1 test material
1.1 given the test agent
Wuji Baifen pian, lot number: D110084, Lerentang Pharmaceutical Factory, Zhongxin Pharmaceutical Group Co., Ltd., Tianj.
WUJI BAIFENG extract VI, lot number: 201301, Zhongxin Innova Laboratories Drug Manufacturing Room.Prepare according to embodiment one
1.2 laboratory animal
Male ICR mouse, female unpregnancy Wistar rat, Beijing Vital River Experimental Animals Technology Co., Ltd. provides, animal quality certification SCXK (capital) 2012-0001.
1.3 medicines and reagent
Aspirin effervescent tablets, lot number: 1208124, AstraZeneca pharmaceutical Co. Ltd.
Sodium carboxymethyl cellulose, lot number: 20120928, Chemical Reagent Co., Ltd., Sinopharm Group.
Dimethylbenzene, lot number: 20100110, Tianjin Kermel Chemical Reagent Co., Ltd..
Diethylstilbestrol injection, lot number: 080621, Tianjin KingYork Amino Acid Co., Ltd..
Oxytocin inj, lot number: 111205, Shanghai Hefeng Pharmaceutical Co., Ltd..
Verapamil hydrochloride injection, lot number: 121101, Shanghai Hefeng Pharmaceutical Co., Ltd..
Sodium chloride, lot number: 20130502, Chemical Reagent Co., Ltd., Sinopharm Group.
Potassium chloride, lot number: F20100728, Chemical Reagent Co., Ltd., Sinopharm Group.
Glucose, lot number: 20120525, Chemical Reagent Co., Ltd., Sinopharm Group.
Magnesium chloride, lot number: 20050401, Tianjin is large chemical reagent development centre forever.
Sodium bicarbonate, lot number: 20110413, Tianjin Kermel Chemical Reagent Co., Ltd..
Anhydrous calcium chloride, lot number: 20020105, Tianjin northization glass purchase and sale center.
Nutritional solution De-Jalon ' s sol: to configure 1L, weighs NaCl9g, KCl0.42g, NaHCO
30.5g, glucose 0.5g are dissolved in appropriate new boiling water, weigh CaCl
20.06g, MgCl
20.005g dissolves in appropriate new boiling water respectively, merges solution, is settled to 1L.
1.4 instrument and equipment
Electronic balance, prunus mume (sieb.) sieb.et zucc. Teller AE240.
Ear is swollen makes ear device, YLS-Q4, Jinan Yi Yan development in science and technology company limited.
HV-4 in vitro tissue organ constant temperature perfusion system, Chengdu TME Technology Co., Ltd..
HW-1000 thermostatic water-circulator bath, Chengdu TME Technology Co., Ltd..
BL-420S biological functional system, Chengdu TME Technology Co., Ltd..
JH-2 muscular tension sensor, Beijing Aviation engineering in medicine institute.
2 test methods and result
2.1 WUJI BAIFENG extract VI xylol bring out the impact of mice ear
Mice is divided into 8 groups at random by body weight, that is: model control group, positive drug control group (aspirin 0.3g/kg), the basic, normal, high dosage group of WUJI BAIFENG extract VI (2.5,5,10g crude drug/kg), often organize 10, continuous gastric infusion 2 weeks, administration volume is 20ml/kg, and model control group and positive drug control group give equal-volume 0.5% sodium carboxymethyl cellulose, and positive drug control group is in causing inflammation gavage aspirin on the same day once.Within after the administration of each treated animal last 1 hour, cause inflammation, auris dextra two sides is only coated with 100% dimethylbenzene 20ul/, left ear is contrast, mice is put to death after causing scorching 45min, two ears are cut along auricle baseline, make ear device lay round auricle at same position respectively with 8mm diameter ear is swollen, use electronic balance weighing weight in wet base, and calculate swelling and suppression ratio.Test data adopts Excel to carry out t-inspection, and result of the test refers to table 1.
The left auricle weight mg of swelling mg=auris dextra sheet weight mg-
Table 1 WUJI BAIFENG extract VI xylol brings out the impact of mice ear
Compare with model control group, * P < 0.05, * * P < 0.01
As shown in Table 1, compare with model control group, WUJI BAIFENG extract VI each administration group mice auricle swelling degree obviously alleviates, and all has pole significant difference (P < 0.01), suppression ratio, all about 40%, has no pronounced amount effect relationship trend; Under same dose, the suppression ratio difference of two samples is very not remarkable.The mice ear that prompting Wuji Baifen pian and the equal xylol of WUJI BAIFENG extract VI bring out has certain inhibitory action.
2.2 Wuji Baifen pians and WUJI BAIFENG extract VI bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Female unpregnancy Wistar rat, every day every subcutaneous injection diethylstilbestrol 2mg/kg, successive administration 3 days, impels animal to be in proestrus or rutting period, to improve the sensitivity of uterus to medicine.4th day, cervical dislocation put to death rat, cuts abdominal cavity open rapidly, peeled off uterus surrounding tissue gently, clip uterus, be placed in pre-cooling through gaseous mixture (95%O
2and 5%CO
2) clean in saturated nutritional solution.Clip is about 1cm uterus section, and two ends are threading respectively, and be little ring, nearly ovary end is hung on tonotransducer after being connected with the elongated hook of L shape, and nearly cervix uteri end is fixed on ventilation hook place, and place 30ml nutritional solution in bath, operating temperature keeps 37 DEG C, continuously logical 95%O
2and 5%CO
2mist.
Adjustment of tonicity sensor, slowly increase uterus tension force, load remains on about 0.5g tension stability, balance about 1 hour, within every 20 minutes, change one time of nutrition liquid, start test when uterus autorhythm shrinking law, trace one section of normal uterus curve movement in contrast.Oxytocin 0.01U/ml is added in bath, uterine muscle strips is caused to shrink, act on and add relative medicine after 30 minutes, adopt accumulation method for dosing medicine to make Wuji Baifen pian and WUJI BAIFENG extract VI final concentration be respectively 2,4,8mg crude drug/ml (nutrient solution), dosing interval 30min.Vehicle controls group gives isopyknic nutritional solution, and positive drug group gives verapamil hydrochloride 1O
-6mol/L, action time is 30min.Use the automatic acquisition of image data of BL-420S biological functional system software.Uterine contraction mean tension, peak-to-peak value are added up, and calculates suppression ratio.Test data uses Excel to carry out t-inspection, and result of the test refers to Fig. 1-3 and table 2.
Table 2 Wuji Baifen pian and WUJI BAIFENG extract VI bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Compare with normal group,
△ △p < 0.01; Compare with oxytocin group, * * P < 0.01, * P < 0.05; Compare with Vehicle controls group, ##P < 0.01, #P < 0.05
From Fig. 1-3 and table 2, the uterine smooth muscle of normal contraction adds oxytocin after-contraction to be strengthened, mean tension, peak-to-peak value all obviously increase, there is pole significant difference (P < 0.01), illustrate that oxytocin can bring out Isolated Rat Uterus smooth muscle contraction spasm, modeling success; After modeling, add nutritional solution, specimen indices, all without significant change, illustrates that nutritional solution as the solvent of dissolved substance, can not affect this test; After modeling, method of cumulative scale adds the Wuji Baifen pian of variable concentrations, specimen decreases before shrinking comparatively dosing, mean tension, peak-to-peak value are all remarkable in oxytocin group, middle and high dosage group has significant difference (P < 0.01, P < 0.05), Testing index all presents obvious dose-effect relationship; After modeling, method of cumulative scale adds the WUJI BAIFENG extract VI of variable concentrations, specimen decreases before shrinking comparatively dosing, mean tension, peak-to-peak value are all remarkable in oxytocin group, middle and high dosage group has pole significant difference (P < 0.01), and Testing index and suppression ratio all present certain dose-effect relationship; Under same dose, WUJI BAIFENG extract VI group suppression ratio is all higher than Wuji Baifen pian group.Prompting Wuji Baifen pian and WUJI BAIFENG extract VI all bring out Isolated Rat Uterus smooth muscle contraction spasm to oxytocin and have certain inhibitory action.
3 conclusions
This test paper examines 2.5,5, the 10g crude drug/continuous gastric infusion of kg WUJI BAIFENG extract VI 2 weeks xylol brings out the impact of mice ear, and 2,4,8mg crude drug/ml Wuji Baifen pian and WUJI BAIFENG extract VI shrink the impact of spasm to the Isolated Rat Uterus that oxytocin is brought out.Result of the test shows: under this experiment condition, 2.5g crude drug/kg and the inflammatory reaction of above dosage WUJI BAIFENG extract VI to mice ear have certain inhibitory action, all do not show significant dose-effect relationship trend; 4mg crude drug/ml and above dosage Wuji Baifen pian and WUJI BAIFENG extract VI have certain inhibitory action to the Isolated Rat Uterus spasm that oxytocin is brought out, and dose-effect relationship is remarkable.Prompting Wuji Baifen pian and WUJI BAIFENG extract VI all have the pharmacological action of inflammation-inhibiting, and under same dose, suppression ratio difference is very not remarkable; Wuji Baifen pian and WUJI BAIFENG extract VI all have the pharmacological action to uterus spasmolytic, and act under same dose, and WUJI BAIFENG extract VI suppression ratio is slightly high.
Test two preparation process researchs
1, granule
Configuration different concentration ethanol is as wetting agent, and adjuvant selects Icing Sugar, lactose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, trial-production granule.Extract powder prepared by different process screens prescription respectively, and carries out rules of preparations inspection to obtained granule.
Method for making: extract powder crosses 80 mesh sieves, adds appropriate amount of auxiliary materials according to Formulation, mixs homogeneously with extract powder, use the ethanol of variable concentrations as wetting agent, soft material processed, 14 mesh sieves are granulated, and 50-60 DEG C of drying, 10 mesh sieve granulate, to obtain final product.
Good preparation prescription and investigation the results are shown in Table 3, table 4
Table 3 Wujibaifeng granules preparation prescription
Table 4 Wujibaifeng granules check result
| Title | Granularity | Color | Mouthfeel | Moisture (%) | Melting |
| 1-3 | Qualified | Dark brown | Slightly sweet micro-raw meat | 4.12 | All dissolve |
| 1-12 | Qualified | Dark brown | Sweet micro-raw meat | 2.30 | All dissolve |
| 2-4 | Qualified | Brown | Slightly sweet micro-raw meat | 3.34 | All dissolve |
| 2-5 | Qualified | Brown | Slightly sweet micro-raw meat | 3.65 | All dissolve |
| 3-6 | Qualified | Dark brown | Slightly sweet micro-raw meat | 4.48 | There is a little precipitation |
| 3-7 | Qualified | Brown | Sweet micro-raw meat | 2.94 | There is a little precipitation |
| 4-4 | Qualified | Brown | Slightly sweet micro-raw meat | 3.18 | There is a little precipitation |
| 4-6 | Qualified | Brown | Slightly sweet micro-raw meat | 2.65 | There is a little precipitation |
| 5-4 | Qualified | Dark brown | Sweet micro-raw meat | 2.36 | There is a little precipitation |
| 6-5 | Qualified | Brown | Slightly sweet micro-raw meat | 2.44 | There is a little precipitation |
| 7-1 | Qualified | Brown | Slightly bitter micro-raw meat | 3.19 | A little precipitation float |
| 7-6 | Qualified | Brown | Sweet micro-raw meat | 3.64 | A little precipitation float |
2 tablets
The extract powder that seven preparation technologies obtain, the sample that wherein technique 1,2 is obtained is full extract powder, and the sample that technique 3-7 obtains contains fecula.
Configuration different concentration ethanol is as wetting agent, and adjuvant selects starch, lactose, microcrystalline Cellulose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, and trial-production granule, adds magnesium stearate, Pulvis Talci tabletting.Extract powder prepared by different process screens prescription respectively, and carries out rules of preparations inspection to obtained tablet.
Method for making: extract powder crosses 80 mesh sieves, appropriate amount of auxiliary materials is added according to Formulation, mix homogeneously with extract powder, use the ethanol of variable concentrations as wetting agent, soft material processed, 16 mesh sieves are granulated, 50-60 DEG C of drying, 14 mesh sieve granulate, additional appropriate magnesium stearate, Pulvis Talci, regulate pressure, loading amount tabletting, to obtain final product.
Good preparation prescription and investigation the results are shown in Table 5, table 6.
Table 5 Wuji Baifen pian preparation prescription
Table 6 Wuji Baifen pian check result
| Title | Moisture (%) before tabletting | Sheet heavy (g) | Weight differential | Hardness | Disintegrate (min) |
| 1-8 | 3.01 | 0.5 | Qualified | 9 | 10 |
| 1-11 | 3.07 | 0.5 | Qualified | 6 | 10 |
| 2-2 | 6.85 | 0.5 | Qualified | 7 | 6 |
| 2-4 | 5.65 | 0.5 | Qualified | 7.5 | 9 |
| 3-2 | 4.44 | 0.5 | Qualified | 7 | 10 |
| 3-3 | 4.85 | 0.5 | Qualified | 7 | 10 |
| 4-4 | 3.27 | 0.5 | Qualified | 7 | 9 |
| 4-5 | 4.25 | 0.5 | Qualified | 7 | 11 |
| 5-1 | 5.02 | 0.5 | Qualified | 8 | 13 |
| 5-4 | 4.75 | 0.5 | Qualified | 6.5 | 8 |
| 6-2 | 5.12 | 0.5 | Qualified | 7 | 8 |
| 6-4 | 5.05 | 0.5 | Qualified | 9 | 10 |
| 7-4 | 5.73 | 0.5 | Qualified | 7 | 13 |
| 7-5 | 4.28 | 0.5 | Qualified | 7 | 14 |
3 capsules
Configuration different concentration ethanol is as wetting agent, and adjuvant selects starch, lactose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, trial-production granule, encapsulating capsule.Extract powder prepared by different process screens prescription respectively, and carries out rules of preparations inspection to obtained capsule.
Method for making: extract powder crosses 80 mesh sieves, adds appropriate amount of auxiliary materials according to Formulation, mixs homogeneously with extract powder, uses the ethanol of variable concentrations as wetting agent, soft material processed, 24 mesh sieves are granulated, 50-60 DEG C of drying, 24 mesh sieve granulate, No. 1, fill and No. 0 capsule, to obtain final product respectively.
Good preparation prescription and investigation the results are shown in Table 7, table 8.
Table 7 WUJI BAIFENG capsule preparations prescription
Table 8 WUJI BAIFENG capsule check result
Beneficial effect of the present invention:
WUJI BAIFENG compositions involved in the present invention, said composition determined curative effect, energy is inflammation-inhibiting well, and spasmolysis is obvious.
The present invention adopts gradient elution successively, and can be eluted by active component completely, yield is high, and extractive content is stablized, and product quality is controlled.In prior art, be all extract once with resin column, extraction ratio is not high, and the active component of plant does not extract completely completely, and residue is discarded, and is applied in suitability for industrialized production and needs medical material more, destroys plant natural environment.Adopt preparation method of the present invention, extraction ratio is high, avoids the waste on the energy.Different concentration ethanol extract powder of the present invention, namely prepares the extract of different content with Same Way, can be used alone according to the technological requirement of preparation also can merge use, reduces cost, is more conducive to suitability for industrialized production.Opposed polarity solvent of the present invention is to resin column repeatedly eluting, and contaminating impurity is few, and resin is easy to recover to reclaim, and recycling, reduces costs, be conducive to environmental protection.Avoid a large amount of as petroleum ether in the present invention, ethyl acetate, the use of the organic solvents such as normal hexane, reduces environmental pollution, decreases the infringement of chemical solvent to the body and mind of staff.Only be the use of the ethanol of water and variable concentrations in the present invention, drop to minimum by toxicity and hazardness, production cost is low simultaneously, is applicable to large-scale industrial production.Preparation method of the present invention, preparation process is simple, and operability is good, easily grasps, favorable reproducibility.
Accompanying drawing illustrates:
Fig. 1 nutritional solution and verapamil hydrochloride bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm to oxytocin
Fig. 2 Wuji Baifen pian (WB) brings out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Fig. 3 WUJI BAIFENG extract VI (WB-6) brings out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Specific embodiment
Embodiment 1,
(1) weight portion medical material is got: Gallus Domesticus extract 40 parts, Colla cornus cervi 108 parts, Carapax Trionycis (processed with vinegar) 54 parts, Concha Ostreae (calcined) 40 parts, Ootheca Mantidis 40 parts, Radix Ginseng 108 parts, the Radix Astragali 27 parts, Radix Angelicae Sinensis 125 parts, the Radix Paeoniae Alba 108 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Asparagi 54 parts, 27 parts, Radix Glycyrrhizae, Radix Rehmanniae 215 parts, Radix Rehmanniae Preparata 216 parts, Rhizoma Chuanxiong 54 parts, Radix Stellariae 20 parts, Radix Salviae Miltiorrhizae 108 parts, Rhizoma Dioscoreae 108 parts, Semen Euryales fry 54 parts, Cornu Cervi Degelatinatum 40 parts is for subsequent use; (2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 95% ethanol extraction of 5 times of medical material amounts, extract 2 times, each 3 hours, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column or polyamide column, first wash with water, use 50,70%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 70%, 870%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 95% ethanol extraction 2 times, each 3 hours, extracting solution merged, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 5 times of soak by water, boiling water decocts 4 hours, filter, merge decoction liquor, decoction liquor 0-4 DEG C of stand at low temperature, with filter plates, it is 70% that filtrate is sink to determining alcohol with 85% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
Embodiment 2
Get weight portion medical material: it is for subsequent use that Gallus Domesticus extract 800g, Colla cornus cervi 2160g, Carapax Trionycis (processed with vinegar) 1080g, Concha Ostreae (calcined) 800g, Ootheca Mantidis 800g, Radix Ginseng 108g, Radix Astragali 2160g, Radix Angelicae Sinensis 2500g, Radix Paeoniae Alba 2160g, Rhizoma Cyperi (processed with vinegar) 2200g, Radix Asparagi 1080g, Radix Glycyrrhizae 540g, Radix Rehmanniae 4320g, Radix Rehmanniae Preparata 4320g, Rhizoma Chuanxiong 800g, Radix Stellariae 400g, Radix Salviae Miltiorrhizae 2160g, Rhizoma Dioscoreae 2200g, Semen Euryales fry 1080g, Cornu Cervi Degelatinatum 2160g; Prepare according to the method for embodiment one.
Embodiment 3
Get weight portion medical material: Gallus Domesticus extract 500g, Colla cornus cervi 1000g, Carapax Trionycis (processed with vinegar) 500g, Concha Ostreae (calcined) 300g, Ootheca Mantidis 500g, Radix Ginseng 1000g, Radix Astragali 200g, Radix Angelicae Sinensis 1200g, Radix Paeoniae Alba 1000g, Rhizoma Cyperi (processed with vinegar) 1000g, Radix Asparagi 500g, Radix Glycyrrhizae 200g, Radix Rehmanniae 2000g, Radix Rehmanniae Preparata 2000g, Rhizoma Chuanxiong 500g, Radix Stellariae 200g, Radix Salviae Miltiorrhizae 1000g, Rhizoma Dioscoreae 1000g, Semen Euryales fry 500g, Cornu Cervi Degelatinatum 300g, are ground into fine powder for subsequent use; Prepare according to embodiment 1 method.
Embodiment 4
Get weight portion medical material: Gallus Domesticus extract 600g, Colla cornus cervi 1100g, Carapax Trionycis (processed with vinegar) 600g, Concha Ostreae (calcined) 500g, Ootheca Mantidis 600g, Radix Ginseng 1100g, Radix Astragali 300g, Radix Angelicae Sinensis 1300g, Radix Paeoniae Alba 1100g, Rhizoma Cyperi (processed with vinegar) 1100g, Radix Asparagi 600g, Radix Glycyrrhizae 300g, Radix Rehmanniae 2200g, Radix Rehmanniae Preparata 2200g, Rhizoma Chuanxiong 600g, Radix Stellariae 300g, Radix Salviae Miltiorrhizae 1100g, Rhizoma Dioscoreae 1100g, Semen Euryales fry 600g, Cornu Cervi Degelatinatum 500g, are ground into fine powder for subsequent use; Prepare according to the method for embodiment 1.
Embodiment 5
(1) weight portion medical material is got: it is for subsequent use that Gallus Domesticus extract 40g, Colla cornus cervi 108g, Carapax Trionycis (processed with vinegar) 54g, Concha Ostreae (calcined) 40g, Ootheca Mantidis 40g, Radix Ginseng 108g, Radix Astragali 27g, Radix Angelicae Sinensis 125g, Radix Paeoniae Alba 108g, Rhizoma Cyperi (processed with vinegar) 108g, Radix Asparagi 54g, Radix Glycyrrhizae 27g, Radix Rehmanniae 215g, Radix Rehmanniae Preparata 216g, Rhizoma Chuanxiong 54g, Radix Stellariae 20g, Radix Salviae Miltiorrhizae 108g, Rhizoma Dioscoreae 108g, Semen Euryales fry 54g, Cornu Cervi Degelatinatum 40g;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 70% ethanol extraction of 5 times of medical material amounts, extract 3 times, each 1 hour, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, adds macroporous resin column or polyamide column, first wash with water, use 50%, 60% ethanol elution respectively, 50% eluent discards, and collects 60% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 70% ethanol extraction 1 time, each 3 hours, extracting solution merged, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 4 times of soak by water, boiling water decocts 3 hours, filter, merge decoction liquor, decoction liquor 0-4 DEG C of stand at low temperature, with filter plates, it is 60% that filtrate is sink to determining alcohol with 70% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
Embodiment 6
(1) weight portion medical material is got: it is for subsequent use that Gallus Domesticus extract 40g, Colla cornus cervi 108g, Carapax Trionycis (processed with vinegar) 54g, Concha Ostreae (calcined) 40g, Ootheca Mantidis 40g, Radix Ginseng 108g, Radix Astragali 27g, Radix Angelicae Sinensis 125g, Radix Paeoniae Alba 108g, Rhizoma Cyperi (processed with vinegar) 108g, Radix Asparagi 54g, Radix Glycyrrhizae 27g, Radix Rehmanniae 215g, Radix Rehmanniae Preparata 216g, Rhizoma Chuanxiong 54g, Radix Stellariae 20g, Radix Salviae Miltiorrhizae 108g, Rhizoma Dioscoreae 108g, Semen Euryales fry 54g, Cornu Cervi Degelatinatum 40g;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 60% ethanol extraction of 5 times of medical material amounts, extract 1 time, each 3 hours, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column or polyamide column, first wash with water, use 50%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 80%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 85% ethanol extraction 3 times, each 1 hour, extracting solution merged, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 6 times of soak by water, boiling water decocts 4 hours, filter, merge decoction liquor, decoction liquor 0-4 DEG C of stand at low temperature, with filter plates, it is 85% that filtrate is sink to determining alcohol with 100% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
Embodiment 7
Any one of Example 1-6 prescription obtains Chinese medicine composition according to the preparation method of embodiment one, with dextrin modified rear spraying dry, be ground into fine powder, add the adjuvant spray granulation such as sucrose, dextrin, mannitol, correctives, 60 DEG C of dryings, add appropriate magnesium stearate, make 1000, obtain chewable tablet.
Embodiment 8
Any one of Example 1-6 prescription obtains Chinese medicine composition according to the preparation method of embodiment one, sodium hydroxide 16g, that 50g of citric acid, citric acid 29g, simple syrup 400ml, and 4% ethyl hydroxybenzoate solution 10ml, distilled water adds to 100ml and namely obtains suspensoid.
Embodiment 9
Example 1-6 any one Chinese medicine composition 1000g, calcium carbonate 21000g, starch 2100g, mix calcium carbonate and starch, sieve, then mix with the extractum that appropriate amount of ethanol dilutes, and crosses No. seven sieves, in 60-70 DEG C of oven dry, encapsulated, makes 100,000 capsules altogether.
Embodiment 10
Gelatin 100g, glycerol 30g, water 130g, get gelatin and add suitable quantity of water and make it expand into gelatin solution.The Chinese medicine composition 1000g of Example 1 mixes with edible vegetable oil 3000g, fully stirs to obtain fluid; Put by the gelatin solution prepared in gelatin solution storage tank and control, at 60 DEG C, fluid to be put into liquor tank, liquid Paraffin temperature is advisable with 10-17 DEG C.Room temperature 10-20 DEG C, water dropper temperature is 40-50 DEG C, starts drop pill, dry, wipes ball, packs and obtain soft capsule.
Embodiment 11
The Chinese medicine composition 450g of Example 3, polyethylene glycol 6000, substrate melting Europe adds fine drug powder mix homogeneously, about 80 DEG C insulations, be 3.3mm with internal diameter, external diameter is the dropper of 5.1mm, drips in speed instillation methyl-silicone oil with 60-70di7/fenzhong, collect drop pill, namely obtain drop pill by filter paper sucking-off liquid coolant.
Embodiment 12
Compositions extractum 10g, the sucrose 40g of embodiment 1, wetting agent 50% alcohol granulation, obtain granule according to process for producing granula.
Embodiment 13
Embodiment 1 compositions extractum 2g, microcrystalline Cellulose 1g, 95% ethanol, make tablet according to method for preparing tablet thereof.
Embodiment 14
Embodiment 1 compositions extractum 2g, starch 2g, lactose 0.4g, 95% ethanol, makes granule, in incapsulating.
Claims (10)
1. a Chinese medicine composition, is characterized in that, is to prepare by the following method:
(1) weight portion medical material is got: Gallus Domesticus extract 50-60 part, Colla cornus cervi 100-110 part, Carapax Trionycis (processed with vinegar) 50-60 part, Concha Ostreae (calcined) 30-50 part, Ootheca Mantidis 50-60 part, Radix Ginseng 100-110 part, Radix Astragali 20-30 part, Radix Angelicae Sinensis 120-130 part, Radix Paeoniae Alba 100-110 part, Rhizoma Cyperi (processed with vinegar) 100-110 part, Radix Asparagi 50-60 part, Radix Glycyrrhizae 20-30 part, Radix Rehmanniae 200-220 part, Radix Rehmanniae Preparata 200-220 part, Rhizoma Chuanxiong 50-60 part, Radix Stellariae 20-30 part, Radix Salviae Miltiorrhizae 100-110 part, Rhizoma Dioscoreae 100-110 part, Semen Euryales fry 50-60 part, Cornu Cervi Degelatinatum 30-50 part, are ground into fine powder for subsequent use;
(2) get the Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae ethanol extraction, filtrate crosses macroporous resin, ion, then uses ethanol elution, and collect eluent, drying under reduced pressure, obtains extract 1;
(3) get Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi ethanol extraction, obtain extract 2;
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum water extract-alcohol precipitation, supernatant concentrating under reduced pressure is dry, obtains extract 3;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
2. Chinese medicine composition as claimed in claim 1, it is characterized in that: in described step (1), Gallus Domesticus extract is obtained by following method: the Gallus Domesticus of getting removing feather claw and intestine is cut into block, add 2-5 times of water gaging, boil 0.5-4 hour, use 5-5: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, adjust pH is to 4-8, 40-65 DEG C, be hydrolyzed after 2-8 hour, boil 2-20 minute, centrifugal, supernatant is evaporated to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use.
3. compositions as claimed in claim 1, it is characterized in that: the 60-100% ethanol extraction of the Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 4-6 times of medical material amount in described step (2), extract 1-3 time, each 2-4 hour, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column, first wash with water, use 50-95% ethanol elution again, collect eluent, eluent is evaporated to thick paste, 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
4. compositions as claimed in claim 3, it is characterized in that, in described step (2), add macroporous resin column or polyamide column, first wash with water, use 50,70%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 70%, 80%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1.
5. compositions as claimed in claim 1, it is characterized in that: Rhizoma Chuanxiong in described step (3), Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 70-95% ethanol extraction 1-3 time, each 2-4 hour, extracting solution merges, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
6. compositions as claimed in claim 1, it is characterized in that: Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 4-6 times soak by water in described step (4), boiling water decocts 2-4 hour, filter, merge decoction liquor, decoction liquor stand at low temperature, with filter plates, it is 70-85% that filtrate is sink to determining alcohol with 60-95% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is.
7. a preparation method for Chinese medicine composition, is characterized in that comprising the steps:
(1) weight portion medical material is got: Gallus Domesticus extract 50-60 part, Colla cornus cervi 100-110 part, Carapax Trionycis (processed with vinegar) 50-60 part, Concha Ostreae (calcined) 30-50 part, Ootheca Mantidis 50-60 part, Radix Ginseng 100-110 part, Radix Astragali 20-30 part, Radix Angelicae Sinensis 120-130 part, Radix Paeoniae Alba 100-110 part, Rhizoma Cyperi (processed with vinegar) 100-110 part, Radix Asparagi 50-60 part, Radix Glycyrrhizae 20-30 part, Radix Rehmanniae 200-220 part, Radix Rehmanniae Preparata 200-220 part, Rhizoma Chuanxiong 50-60 part, Radix Stellariae 20-30 part, Radix Salviae Miltiorrhizae 100-110 part, Rhizoma Dioscoreae 100-110 part, Semen Euryales fry 50-60 part, Cornu Cervi Degelatinatum 30-50 part, are ground into fine powder for subsequent use;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, the Radix Glycyrrhizae 60-100% ethanol extraction of 4-6 times of medical material amount, extract 1-3 time, each 2-4 hour, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column, first wash with water, then use 50-95% ethanol elution, collect eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1;
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 70-95% ethanol extraction 1-3 time, each 2-4 hour, extracting solution merges, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2.
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 4-6 times of soak by water, boiling water decocts 2-4 hour, filter, merge decoction liquor, decoction liquor stand at low temperature, with filter plates, it is 70-85% that filtrate is sink to determining alcohol with 60-95% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
8. the preparation method of Chinese medicine composition as claimed in claim 7, is characterized in that: comprise the steps:
(1) weight portion medical material is got: Gallus Domesticus extract 40 parts, Colla cornus cervi 108 parts, Carapax Trionycis (processed with vinegar) 54 parts, Concha Ostreae (calcined) 40 parts, Ootheca Mantidis 40 parts, Radix Ginseng 108 parts, the Radix Astragali 27 parts, Radix Angelicae Sinensis 125 parts, the Radix Paeoniae Alba 108 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Asparagi 54 parts, 27 parts, Radix Glycyrrhizae, Radix Rehmanniae 215 parts, Radix Rehmanniae Preparata 216 parts, Rhizoma Chuanxiong 54 parts, Radix Stellariae 20 parts, Radix Salviae Miltiorrhizae 108 parts, Rhizoma Dioscoreae 108 parts, Semen Euryales fry 54 parts, Cornu Cervi Degelatinatum 40 parts is for subsequent use;
(2) Radix Astragali, the Radix Paeoniae Alba, Radix Asparagi, Radix Ginseng, Radix Stellariae, Radix Glycyrrhizae 95% ethanol extraction of 5 times of medical material amounts, extract 2 times, each 3 hours, filter, it is 1.02-1.20 that filtrate is concentrated into 60 DEG C of relative densities, add macroporous resin column or polyamide column, first wash with water, use 50,70%, 80%, 90% ethanol elution respectively, 50% eluent discards, and collects 70%, 870%, 90% eluent, merge eluent, eluent is evaporated to thick paste, and 60 DEG C of relative densities are 1.02-1.20, dry extract 1;
(3) Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Angelicae Sinensis, Rhizoma Cyperi 95% ethanol extraction 2 times, each 3 hours, extracting solution merged, be evaporated to relative density 60 DEG C and survey 1.2-1.3, add water to stir and place, filter, filtrate is concentrated into relative density 60 DEG C and surveys 1.12-1.15, dry extract 2;
(4) Carapax Trionycis, Ootheca Mantidis, Radix Rehmanniae Preparata, Radix Rehmanniae, Concha Ostreae, Semen Euryales, Rhizoma Dioscoreae, Cornu Cervi Degelatinatum 5 times of soak by water, boiling water decocts 4 hours, filter, merge decoction liquor, decoction liquor 0-4 DEG C of stand at low temperature, with filter plates, it is 70% that filtrate is sink to determining alcohol with 85% ethanol alcohol again, decompression recycling ethanol, the extract 3 that drying is;
(5) namely said extracted thing 1,2,3 Gallus Domesticus extract and Colla cornus cervi powder mix homogeneously are obtained compositions.
9. the preparation containing any one of claim 1-4 Chinese medicine composition, is characterized in that: described Chinese medicine composition is 1-3: 3-1 with adjuvant ratio.
10. the application of compositions as claimed in claim 1 in preparation treatment antiinflammatory or spasmolytic medicine.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1457848A (en) * | 2003-06-11 | 2003-11-26 | 天津中新药业集团股份有限公司乐仁堂制药厂 | Black-bone chicken white phoenix preparation medicine and its preparing method |
Non-Patent Citations (1)
| Title |
|---|
| 吴跃进: "乌鸡白凤丸的药理研究概况", 《中国中医药信息杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727018A (en) * | 2017-01-09 | 2017-05-31 | 江苏康缘药业股份有限公司 | A kind of extracting method of ginseng extract |
| CN106727018B (en) * | 2017-01-09 | 2020-07-24 | 江苏康缘药业股份有限公司 | Method for extracting ginseng extract |
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| Publication number | Publication date |
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| CN104740233B (en) | 2018-05-22 |
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Address after: 300112 No. 2, Daming Road, Xiqing District, Tianjin Patentee after: Lerentang Pharmaceutical Factory of Jinyao Darentang Group Co.,Ltd. Address before: 300112 No. 2, Daming Road, Xiqing District, Tianjin Patentee before: Letrentang pharmaceutical factory of Tianjin Zhongxin Pharmaceutical Group Co.,Ltd. |