CN104740175A - Silky fowl composition and preparation method thereof - Google Patents
Silky fowl composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a silky fowl preparation and a preparation method thereof. The silky fowl preparation comprises the following medicinal materials by weight: 540 parts of unhaired, declawed and intestine removed silky fowl, 108 parts of Salvia Miltiorrhiza, 216 parts of rehmannia glutinosa, 108 parts of vinegar processed cyperus rotundus, 108 parts of ginseng, 108 parts of radix paeoniae alba, 40 parts of calcined oyster, 40 parts of Cornu Cervi Degelatinatum, 22 parts of Radix Stellariae, 27 parts of licorice, 27 parts of astragalus, and 54 parts of processed turtle shell. The simple preparation method includes: silky fowl enzymolysis; turtle shell water extraction and concentration; water extraction of ginseng, radix paeoniae alba and licorice, and purification by macroporous resin; alcohol extraction of Salvia miltiorrhiza, astragalus and Radix Stellariae; water extraction and alcohol precipitation of rehmannia glutinosa, Cornu Cervi Degelatinatum and calcined oyster shell; volatile oil extraction from vinegar processed cyperus rotundus; and combination of all extracts, thus obtaining the pharmaceutical composition.
Description
Technical field
The invention belongs to field of medicaments, especially relate to a kind of Chinese medicine composition and preparation method thereof.
Background technology
WUJI BAIFENG capsule is the WUJI BAIFENG capsule be prepared from according to the preparation method provided in national drug standards WS-11514 (ZD-1514)-2002, that one has benefiting qi and nourishing blood, adjusting meridian and stopping leukorrhea effect, for QI and blood deficiency, asthenic body, soreness of the waist and knees, menoxenia, the syrups oral formulations of the treatment for diseases such as bleeding not during menses, through clinical verification, determined curative effect is the common drug that clinical and family is used for the treatment of above-mentioned disease.The white phoenix capsule of chicken is prepared from according to the preparation method provided in national drug standards WS-11514 (ZD-1514)-2002.Below the composition and engineering to providing in this drug standard and brief description thereof:
Prescription: bird chicken (removing feather claw and intestine) 540g. Radix Rehmanniae 216g, Radix Paeoniae Alba 108g, Radix Salviae Miltiorrhizae 108g, Radix Ginseng 108g, Rhizoma Cyperi (vinegar system) 108g, Radix Astragali 27g, Carapax Trionycis (system) 54g, Radix Stellariae 27g. Concha Ostreae (forging) 40g. Cornu Cervi Degelatinatum 40g. Radix Glycyrrhizae 27g
Method for making: above 12 taste medical materials, Gallus Domesticus is cut into fragment, decocts with water 1h, add paraffin while hot, stir and make it dissolve, placement is spent the night, abandon or adopt upper strata paraffin and oils and fats solid. break into homogenate, add papain to stir, regulate pH value to 6 ~ 7, after 50 DEG C of hydrolysis 5h, boil 5 minutes, centrifugal, supernatant is evaporated to thick paste, for subsequent use;
Radix Salviae Miltiorrhizae adds 70% soak with ethanol and spends the night, reflux secondary, each 30 minutes, and merge extractive liquid, is centrifugal, and supernatant reclaims ethanol and is concentrated into the thick paste that relative density is 1.30 ~ 1.35, for subsequent use;
Medicinal residues and all the other etc. ten tastes decoct with water 3 times, first time 3h, second time 2h, third time 1h, collecting decoction, filter, filtrate is concentrated into the thick paste that relative density is 1.30 ~ 1.35, merges, vacuum drying, be ground into fine powder with above-mentioned thick paste, sieve, mixing, incapsulates, to obtain final product.
Function cures mainly: benefiting qi and nourishing blood, adjusting meridian and stopping leukorrhea; For QI and blood deficiency, asthenic body, soreness of the waist and knees, menoxenia, bleeding not during menses.
Due to preparation technology, the various problems such as above-mentioned WUJI BAIFENG capsule exists that patient's dose is large, impurity content is high in medicine, side reaction probability is large, directly affect therapeutic effect.
Summary of the invention
In order to solve the problem, invention provides a kind of WUJI BAIFENG Chinese medicine composition, and this pharmaceutical composition is through extracting targetedly, and its active constituent content is high, effectively reduces the dose of patient, and has the spasmolysis that well eases pain.
Present invention also offers, the preparation method of this WUJI BAIFENG Chinese medicine composition, to adapt to suitability for industrialized production.
The present invention is achieved by the following technical solutions:
A kind of WUJI BAIFENG Chinese medicine composition, is obtained by following preparation method:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2-5 times of water gaging, boils 0.5-4 hour, use 5-15: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, and adjust pH is to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boils 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 1-3 time, the 2-8 that at every turn adds water doubly, decoct 0.5-3 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 0.5-4 gram of resin, with 3-10 times amount water elution, discard water lotion, use 50%-95% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) to decoct with water 1-3 time, the 6-10 that at every turn adds water doubly, decocts 0.5-4 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 75%-90% ethanol to stir, make alcohol content be 50%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Wherein step 2 is preferred: the Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3-4 times of water gaging, boils 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, and adjust pH is to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boils 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use.
Step 4 is preferred: get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
Step 6 is preferred: get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, and the 7-9 that at every turn adds water doubly, decocts 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, make alcohol content be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
A preparation method for WUJI BAIFENG Chinese medicine composition, is characterized in that, step is as follows:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2-5 times of water gaging, boils 0.5-4 hour, use 5-15: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, and adjust pH is to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boils 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 1-3 time, the 2-8 that at every turn adds water doubly, decoct 0.5-3 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 0.5-4 gram of resin, with 3-10 times amount water elution, discard water lotion, use 50%-95% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) to decoct with water 1-3 time, the 6-10 that at every turn adds water doubly, decocts 0.5-4 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 75%-90% ethanol to stir, make alcohol content be 50%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Preferred preparation method is:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3-4 times of water gaging, boils 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, and adjust pH is to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boils 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, the 7-9 that at every turn adds water doubly, decocts 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, make alcohol content be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Macroporous adsorbent resin wherein described in step 4 is selected from the one in D101 or AB-8.
The present invention also provides the pharmaceutical preparation be prepared into as active constituents of medicine with Chinese medicine composition of the present invention, pharmaceutical preparation of the present invention, and comprising compositions is by weight percentage 0.1-99.9%, and all the other are pharmaceutically acceptable carrier.The weight ratio of preferred Chinese medicine composition extract powder of the present invention and adjuvant is 3: 1-1: 3, and the best is extract powder is 1: 1 with other supplementary product consumption ratios.
Pharmaceutical preparation of the present invention can be any pharmaceutically useful dosage form, conventionally prepare, these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably peroral dosage form, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc., be more preferably capsule, granule, tablet and oral liquid.
Carrier of the present invention is that the carrier pharmaceutically commonly used is selected from mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its derivates, alginate, gelatin, polyvinylpyrrolidone, glycerol, POLYSORBATE 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
All according to weight portion as proportioning in above-mentioned composition of the present invention, aborning can according to corresponding proportion increase or reduce, as large-scale production can by kilogram or ton in units of, small-scale production can be counted in units of mg.Weight can increase or reduce, but the raw medicinal herbs weight proportion ratio between each composition can not change.
Above part by weight of the present invention is through that science filters out, can corresponding increase or reduce the corresponding ratio of adjustment for especial patient such as serious symptom or mild, but increase and decrease or reduce and be no more than 300%, and effect is constant.
Single medical material in above Chinese medicine composition, especially ministerial drug and adjuvant drug can be replaced by the Chinese medicine suitably with the identical property of medicine, and after replacing, the drug effect of Chinese medicine preparation is constant.
The present invention other optimal way in a particular embodiment.
WUJI BAIFENG preparation of the present invention has antiinflammatory and lissive effect.
In order to confirm the above-mentioned beneficial effect of technical solution of the present invention, inventor has done the pharmacodynamic feature of corresponding pharmacodynamics test and contrast test many-side research medicine of the present invention.
Test example one,
1.1 given the test agent
Contrast medicine: WUJI BAIFENG capsule, according to the national drug standards preparation recorded in background technology
Medicine of the present invention: WUJI BAIFENG extract, prepares according to embodiment one
1.2 laboratory animal
Male ICR mouse, female unpregnancy Wistar rat, Beijing Vital River Experimental Animals Technology Co., Ltd. provides, animal quality certification SCXK (capital) 2012-0001.
1.3 medicines and reagent
Aspirin effervescent tablets, lot number: 1208124, AstraZeneca pharmaceutical Co. Ltd.
Sodium carboxymethyl cellulose, lot number: 20120928, Chemical Reagent Co., Ltd., Sinopharm Group.
Dimethylbenzene, lot number: 20100110, Tianjin Kermel Chemical Reagent Co., Ltd..
Diethylstilbestrol injection, lot number: 080621, Tianjin KingYork Amino Acid Co., Ltd..
Oxytocin inj, lot number: 111205, Shanghai Hefeng Pharmaceutical Co., Ltd..
Verapamil hydrochloride injection, lot number: 121101, Shanghai Hefeng Pharmaceutical Co., Ltd..
Sodium chloride, lot number: 20130502, Chemical Reagent Co., Ltd., Sinopharm Group.
Potassium chloride, lot number: F20100728, Chemical Reagent Co., Ltd., Sinopharm Group.
Glucose, lot number: 20120525, Chemical Reagent Co., Ltd., Sinopharm Group.
Magnesium chloride, lot number: 20050401, Tianjin is large chemical reagent development centre forever.
Sodium bicarbonate, lot number: 20110413, Tianjin Kermel Chemical Reagent Co., Ltd..
Anhydrous calcium chloride, lot number: 20020105, Tianjin northization glass purchase and sale center.
Nutritional solution De-Jalon ' s sol: to configure 1L, weighs NaCl9g, KCl0.42g, NaHCO
30.5g, glucose 0.5g are dissolved in appropriate new boiling water, weigh CaCl
20.06g, MgCl
20.005g dissolves in appropriate new boiling water respectively, merges solution, is settled to 1L.
1.4 instrument and equipment
Electronic balance, prunus mume (sieb.) sieb.et zucc. Teller AE240.
Ear is swollen makes ear device, YLS-Q4, Jinan Yi Yan development in science and technology company limited.
HV-4 in vitro tissue organ constant temperature perfusion system, Chengdu TME Technology Co., Ltd..
HW-1000 thermostatic water-circulator bath, Chengdu TME Technology Co., Ltd..
BL-4205 biological functional system, Chengdu TME Technology Co., Ltd..
JH-2 muscular tension sensor, Beijing Aviation engineering in medicine institute.
2 test methods and result
2.1 WUJI BAIFENG capsules and WUJI BAIFENG extract xylol bring out the impact of mice ear
Mice is divided into 8 groups at random by body weight, that is: model control group, positive drug control group (aspirin 0.3g/kg), WUJI BAIFENG capsule and the basic, normal, high dosage group of WUJI BAIFENG extract (2.5,5,10g crude drug/kg), often organize 10, continuous gastric infusion 2 weeks, administration volume is 20ml/kg, and model control group and positive drug control group give equal-volume 0.5% sodium carboxymethyl cellulose, and positive drug control group is in causing inflammation gavage aspirin on the same day once.Within after the administration of each treated animal last 1 hour, cause inflammation, auris dextra two sides is only coated with 100% dimethylbenzene 20ul/, left ear is contrast, mice is put to death after causing scorching 45min, two ears are cut along auricle baseline, make ear device lay round auricle at same position respectively with 8mm diameter ear is swollen, use electronic balance weighing weight in wet base, and calculate swelling and suppression ratio.Test data adopts Excel to carry out t-inspection, and result of the test refers to table 1.
The left auricle weight mg of swelling mg=auris dextra sheet weight mg-
Table 1 WUJI BAIFENG capsule and WUJI BAIFENG extract xylol bring out the impact of mice ear
Compare with model control group, * P < 0.05, * * P < 0.01
Note: WUJI BAIFENG extract middle and high dosage group respectively have a mice because of gavage operate miss lethal.
As shown in Table 1, compare with model control group, WUJI BAIFENG extract each administration group mice auricle swelling degree obviously alleviates, and has pole significant difference (P < 0.01), suppression ratio, all higher than 30%, has no pronounced amount effect relationship trend; WUJI BAIFENG capsule each administration group mice auricle swelling degree alleviates to some extent, but only high dose group has significant difference (P < 0.05); Under same dose, WUJI BAIFENG extract group suppression ratio is higher than WUJI BAIFENG Capsules group.The mice ear that prompting WUJI BAIFENG capsule and the equal xylol of WUJI BAIFENG extract bring out has certain inhibitory action.
2.2 WUJI BAIFENG capsules and WUJI BAIFENG extract bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm to oxytocin
Female unpregnancy Wistar rat, every day every subcutaneous injection diethylstilbestrol 2mg/kg, successive administration 3 days, impels animal to be in proestrus or rutting period, to improve the sensitivity of uterus to medicine.4th day, cervical dislocation put to death rat, cuts abdominal cavity open rapidly, peeled off uterus surrounding tissue gently, clip uterus, be placed in pre-cooling through gaseous mixture (95%O
2and 5%CO
2) clean in saturated nutritional solution.Clip is about 1cm uterus section, and two ends are threading respectively, and be little ring, nearly ovary end is hung on tonotransducer after being connected with the elongated hook of L shape, and nearly cervix uteri end is fixed on ventilation hook place, and place 30ml nutritional solution in bath, operating temperature keeps 37 DEG C, continuously logical 95%O
2and 5%CO
2mist.
Adjustment of tonicity sensor, slowly increase uterus tension force, load remains on about 0.5g tension stability, balance about 1 hour, within every 20 minutes, change one time of nutrition liquid, start test when uterus autorhythm shrinking law, trace one section of normal uterus curve movement in contrast.Oxytocin 0.01U/ml is added in bath, uterine muscle strips is caused to shrink, act on and add relative medicine after 30 minutes, adopt accumulation method for dosing medicine to make WUJI BAIFENG capsule and WUJI BAIFENG extract final concentration be respectively 2,4,8mg crude drug/ml (nutrient solution), dosing interval 30min.Vehicle controls group gives isopyknic nutritional solution, and positive drug group gives verapamil hydrochloride 10
-6mol/L, action time is 30min.Use the automatic acquisition of image data of BL-420S biological functional system software.Uterine contraction mean tension, peak-to-peak value are added up, and calculates suppression ratio.Test data uses Excel to carry out t-inspection, and result of the test refers to Fig. 1-3 and table 2.
Table 2 WUJI BAIFENG capsule and WUJI BAIFENG extract bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm to oxytocin
Compare with normal group,
Δ Δp < 0.01; Compare with oxytocin group, * * P < 0.01; Compare with Vehicle controls group, ##P < 0.01, #P < 0.05
From Fig. 1-3 and table 2, the uterine smooth muscle of normal contraction adds oxytocin after-contraction to be strengthened, mean tension, peak-to-peak value all obviously increase, there is pole significant difference (P < 0.01), illustrate that oxytocin can bring out Isolated Rat Uterus smooth muscle contraction spasm, modeling success; After modeling, add nutritional solution, specimen indices, all without significant change, illustrates that nutritional solution as the solvent of dissolved substance, can not affect this test; After modeling, method of cumulative scale adds the WUJI BAIFENG capsule of variable concentrations, specimen decreases before shrinking comparatively dosing, mean tension, peak-to-peak value are all remarkable in oxytocin group, middle and high dosage group has pole significant difference (P < 0.01), and Testing index and suppression ratio all present obvious dose-effect relationship; After modeling, method of cumulative scale adds the WUJI BAIFENG extract of variable concentrations, specimen decreases before shrinking comparatively dosing, mean tension, peak-to-peak value are all lower than oxytocin group, but only the index of high dose group all has pole significant difference (P < 0.01), and Testing index and suppression ratio all present certain dose-effect relationship; Under same dose, WUJI BAIFENG extract group suppression ratio is all higher than WUJI BAIFENG Capsules group.Prompting WUJI BAIFENG capsule and WUJI BAIFENG extract all bring out Isolated Rat Uterus smooth muscle contraction spasm to oxytocin and have certain inhibitory action.
3 conclusions
This test paper examines 2.5,5,10g crude drug/kg WUJI BAIFENG capsule and the continuous gastric infusion of WUJI BAIFENG extract 2 weeks xylol bring out the impact of mice ear, and 2,4,8mg crude drug/ml WUJI BAIFENG capsule and WUJI BAIFENG extract shrink the impact of spasm to the Isolated Rat Uterus that oxytocin is brought out.Result of the test shows: under this experiment condition, and 2.5g crude drug/kg and above dosage WUJI BAIFENG extract and the 10g crude drug/inflammatory reaction of kg WUJI BAIFENG capsule to mice ear have certain inhibitory action; 4mg crude drug/ml and above dosage WUJI BAIFENG extract and 8mg crude drug/ml WUJI BAIFENG capsule have certain inhibitory action to the Isolated Rat Uterus spasm that oxytocin is brought out, and dose-effect relationship is remarkable.Prompting WUJI BAIFENG extract and WUJI BAIFENG capsule all have inflammation-inhibiting and the pharmacological action to uterus spasmolytic, and under same dose, the suppression ratio of WUJI BAIFENG extract is slightly high.
Test two preparation process researchs
1, granule
Configuration different concentration ethanol is as wetting agent, and adjuvant selects Icing Sugar, lactose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, trial-production granule.Extract powder prepared by different process screens prescription respectively, and carries out rules of preparations inspection to obtained granule.
Method for making: extract powder crosses 80 mesh sieves, adds appropriate amount of auxiliary materials according to Formulation, mixs homogeneously with extract powder, use the ethanol of variable concentrations as wetting agent, soft material processed, 14 mesh sieves are granulated, and 50-60 DEG C of drying, 10 mesh sieve granulate, to obtain final product.
Good preparation prescription and investigation the results are shown in Table 3, table 4
Table 3 Wujibaifeng granules preparation prescription
Table 4 Wujibaifeng granules check result
| Title | Granularity | Color | Mouthfeel | Moisture (%) | Melting |
| 1-3 | Qualified | Dark brown | Slightly sweet micro-raw meat | 4.12 | All dissolve |
| 1-12 | Qualified | Dark brown | Sweet micro-raw meat | 2.30 | All dissolve |
| 2-4 | Qualified | Brown | Slightly sweet micro-raw meat | 3.34 | All dissolve |
| 2-5 | Qualified | Brown | Slightly sweet micro-raw meat | 3.65 | All dissolve |
| 3-6 | Qualified | Dark brown | Slightly sweet micro-raw meat | 4.48 | There is a little precipitation |
| 3-7 | Qualified | Brown | Sweet micro-raw meat | 2.94 | There is a little precipitation |
| 4-4 | Qualified | Brown | Slightly sweet micro-raw meat | 3.18 | There is a little precipitation |
| 4-6 | Qualified | Brown | Slightly sweet micro-raw meat | 2.65 | There is a little precipitation |
| 5-4 | Qualified | Dark brown | Sweet micro-raw meat | 2.36 | There is a little precipitation |
| 6-5 | Qualified | Brown | Slightly sweet micro-raw meat | 2.44 | There is a little precipitation |
| 7-1 | Qualified | Brown | Slightly bitter micro-raw meat | 3.19 | A little precipitation float |
| 7-6 | Qualified | Brown | Sweet micro-raw meat | 3.64 | A little precipitation float |
2 tablets
Configuration different concentration ethanol is as wetting agent, and adjuvant selects starch, lactose, microcrystalline Cellulose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, and trial-production granule, adds magnesium stearate, Pulvis Talci tabletting.
Method for making: extract powder crosses 80 mesh sieves, appropriate amount of auxiliary materials is added according to Formulation, mix homogeneously with extract powder, use the ethanol of variable concentrations as wetting agent, soft material processed, 16 mesh sieves are granulated, 50-60 DEG C of drying, 14 mesh sieve granulate, additional appropriate magnesium stearate, Pulvis Talci, regulate pressure, loading amount tabletting, to obtain final product.
Good preparation is investigated and be the results are shown in Table 5, table 6.
Table 5 Wuji Baifen pian preparation prescription
Table 6 Wuji Baifen pian check result
3 capsules
Configuration different concentration ethanol is as wetting agent, and adjuvant selects starch, lactose, adjustment auxiliary material proportion and concentration of wetting agent and consumption, trial-production granule, encapsulating capsule.Extract powder prepared by different process screens prescription respectively, and carries out rules of preparations inspection to obtained capsule.
Method for making: extract powder crosses 80 mesh sieves, adds appropriate amount of auxiliary materials according to Formulation, mixs homogeneously with extract powder, uses the ethanol of variable concentrations as wetting agent, soft material processed, 24 mesh sieves are granulated, 50-60 DEG C of drying, 24 mesh sieve granulate, No. 1, fill and No. 0 capsule, to obtain final product respectively.
Good preparation prescription and investigation the results are shown in Table 7, table 8.
Table 7 WUJI BAIFENG capsule preparations prescription
Table 8 WUJI BAIFENG capsule check result
Beneficial effect of the present invention:
WUJI BAIFENG preparation involved in the present invention, said preparation determined curative effect, energy is inflammation-inhibiting well, and spasmolysis is obvious.
Invent the difference for each component in the WUJI BAIFENG capsule described in background technology, through exploratory development, have employed Gallus Domesticus enzymolysis respectively; Carapax Trionycis water extraction concentrates; Purification by macroporous resin is crossed after Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae water extraction; Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae alcohol extraction; Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) water extract-alcohol precipitation; Rhizoma Cyperi (processed with vinegar) extracts volatile oil, to improve the extraction ratio of effective ingredient, reduces the incidence rate of dose and side reaction.
Only be the use of the ethanol of water and variable concentrations in the present invention, drop to minimum by toxicity and hazardness, production cost is low simultaneously, is applicable to large-scale industrial production.Preparation method of the present invention, preparation process is simple, and operability is good, easily grasps, favorable reproducibility.
In order to simple and clear technical scheme of the present invention; pharmacodynamics and the contrast test data of embodiment 1 are only listed in description; the compositions of other embodiments and claim 1 protection domain can both realize experiment purpose of the present invention, has the effect of antiinflammatory and spasmolytic.
Accompanying drawing explanation
Fig. 1 nutritional solution and verapamil hydrochloride bring out the impact of Isolated Rat Uterus smooth muscle contraction spasm to oxytocin
Fig. 2 WUJI BAIFENG extract (WB-2) brings out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Fig. 3 WUJI BAIFENG capsule (WB) brings out the impact of Isolated Rat Uterus smooth muscle contraction spasm on oxytocin
Specific embodiment
Embodiment 1
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3-4 times of water gaging, boils 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, and adjust pH is to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boils 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, the 7-9 that at every turn adds water doubly, decocts 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, make alcohol content be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 2
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3-4 times of water gaging, boils 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, and adjust pH is to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boils 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, the 7-9 that at every turn adds water doubly, decocts 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, make alcohol content be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 3
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 4 times of water gagings, boils 3.5 hours, use 10: 1 hard paraffin defats while hot, the papain adding 400: 1 afterwards stirs, adjust pH to 6,65 DEG C, be hydrolyzed after 7 hours, boil 15 minutes, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.32, at 90 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 3 times, add water 5 times at every turn, decoct 4 hours, filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.32, and at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decoct with water 2 times, add water 6 times at every turn, decoct 2.5 hours, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.20, add processed good macroporous adsorbent resin, every gram of medical material 2.5 grams of resins, with 8 times amount water elutions, discard water lotion, use 90% ethanol elution again, check that, without saponin reaction, eluent decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.34 to eluent, at 90 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 80%, reflux, extract, 3 times, add ethanol 8 times amount at every turn, reflux 3 hours, filter, merging filtrate, decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.32, at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, add water 9 times at every turn, decoct 3.5 hours, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.35 to relative density 60 DEG C, add 90% ethanol to stir, make alcohol content be 70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.32, at 80 DEG C, and 0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 8 times of water gagings, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 4
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3 times of water gagings, boils 1.5 hours, use 15: 1 hard paraffin defats while hot, the papain adding 100: 1 afterwards stirs, adjust pH to 5,40 DEG C, be hydrolyzed after 3 hours, boil 5 minutes, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02, at 60 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1 time, add water 2 times at every turn, decoct 0.5 hour, filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02, and at 50 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decoct with water 2 times, add water 3 times at every turn, decoct 1.5 hours, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02, add processed good macroporous adsorbent resin, every gram of medical material 1.5 grams of resins, with 4 times amount water elutions, discard water lotion, use 60% ethanol elution again, check that, without saponin reaction, eluent decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.02 to eluent, at 60 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%, reflux, extract, 1 time, add ethanol 2 times amount at every turn, reflux 0.5 hour, filter, merging filtrate, decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.02, at 50 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, add water 7 times at every turn, decoct 1.5 hours, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02 to relative density 60 DEG C, add 80% ethanol to stir, make alcohol content be 60%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02, at 60 DEG C, and 0.02Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4 times of water gagings, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 5
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 5 times of water gagings, boils 4 hours, use 15: 1 hard paraffin defats while hot, the papain adding 500: 1 afterwards stirs, adjust pH to 8,65 DEG C, be hydrolyzed after 8 hours, boil 20 minutes, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.32, at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 3 times, add water 5 times at every turn, decoct 4 hours, filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.32, and at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decoct with water 3 times, add water 8 times at every turn, decoct 3 hours, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.20, add processed good macroporous adsorbent resin, every gram of medical material 4 grams of resins, with 10 times amount water elutions, discard water lotion, use 95% ethanol elution again, check that, without saponin reaction, eluent decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.34 to eluent, at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 80%, reflux, extract, 3 times, add ethanol 8 times amount at every turn, reflux 3 hours, filter, merging filtrate, decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.32, at 100 DEG C, 0.12Mpa vacuum drying, gets dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 3 times, add water 10 times at every turn, decoct 4 hours, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.35 to relative density 60 DEG C, add 90% ethanol to stir, make alcohol content be 70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.32, at 100 DEG C, and 0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 8 times of water gagings, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 6
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2 times of water gagings, boils 0.5 hour, use 5: 1 hard paraffin defats while hot, the papain adding 40: 1 afterwards stirs, adjust pH to 4,40 DEG C, be hydrolyzed after 2 hours, boil 2 minutes, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02, at 50 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1 time, add water 2 times at every turn, decoct 0.5 hour, filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02, and at 50 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
4) get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decoct with water 1 time, add water 2 times at every turn, decoct 0.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02, add processed good macroporous adsorbent resin, every gram of medical material 0.5 gram of resin, with 3 times amount water elutions, discards water lotion, use 50%% ethanol elution again, check that, without saponin reaction, eluent decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.02 to eluent, at 50 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%, reflux, extract, 1 time, add ethanol 2 times amount at every turn, reflux 0.5 hour, filter, merging filtrate, decompression recycling ethanol is to thick paste, and relative density 60 DEG C surveys 1.02, at 50 DEG C, 0.02Mpa vacuum drying, gets dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 1 time, add water 6 times at every turn, decoct 0.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02 to relative density 60 DEG C, add 75% ethanol to stir, make alcohol content be 50%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02, at 50 DEG C, and 0.02Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4 times of water gagings, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 7
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2-5 times of water gaging, boils 0.5-4 hour, use 5-15: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, and adjust pH is to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boils 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 1-3 time, the 2-8 that at every turn adds water doubly, decoct 0.5-3 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 0.5-4 gram of resin, with 3-10 times amount water elution, discard water lotion, use 50%-95% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) to decoct with water 1-3 time, the 6-10 that at every turn adds water doubly, decocts 0.5-4 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 75%-90% ethanol to stir, make alcohol content be 50%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
Embodiment 8
The pharmaceutical composition thing extractum 10g of any one of Example 1-7 gained, sucrose 15g, lactose 15g, wetting agent 60% alcohol granulation, obtains granule according to process for producing granula.
Embodiment 9
Drug extract 1.44g, starch 0.56g, 90% ethanol of real any one of Example 1-7 gained, make tablet according to method for preparing tablet thereof.
Embodiment 10
Drug extract 1.44g, the starch 0.56g of any one of Example 1-7 gained, 90% ethanol, makes granule, in incapsulating.
Claims (10)
1. a WUJI BAIFENG Chinese medicine composition, is characterized in that, is obtained by following preparation method:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2-5 times of water gaging, boils 0.5-4 hour, use 5-15: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, and adjust pH is to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boils 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 1-3 time, the 2-8 that at every turn adds water doubly, decoct 0.5-3 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 0.5-4 gram of resin, with 3-10 times amount water elution, discard water lotion, use 50%-95% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) to decoct with water 1-3 time, the 6-10 that at every turn adds water doubly, decocts 0.5-4 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 75%-90% ethanol to stir, make alcohol content be 50%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
2. Chinese medicine composition as claimed in claim 1, it is characterized in that, step described in step 2 is: the Gallus Domesticus of getting removing feather claw and intestine is cut into block, add 3-4 times of water gaging, boil 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, adjust pH, to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boil 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use.
3. Chinese medicine composition as claimed in claim 1, it is characterized in that, step described in step 4 is: get broken Radix Ginseng, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use.
4. Chinese medicine composition as claimed in claim 1, it is characterized in that, step described in step 6 is: get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) decoct with water 2 times, the 7-9 that at every turn adds water doubly, decoct 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, alcohol content is made to be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use.
5. a preparation method for WUJI BAIFENG Chinese medicine composition, is characterized in that, step is as follows:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 2-5 times of water gaging, boils 0.5-4 hour, use 5-15: 1 hard paraffin defat while hot, the papain adding 40-500: 1 afterwards stirs, and adjust pH is to 4-8,40-65 DEG C, is hydrolyzed after 2-8 hour, boils 2-20 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 1-3 time, the 2-8 that at every turn adds water doubly, decoct 0.5-3 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 0.5-4 gram of resin, with 3-10 times amount water elution, discard water lotion, use 50%-95% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) to decoct with water 1-3 time, the 6-10 that at every turn adds water doubly, decocts 0.5-4 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 75%-90% ethanol to stir, make alcohol content be 50%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
6. preparation method as claimed in claim 5, it is characterized in that, step is as follows:
1) weight portion medical material is got: removing feather claw and intestine Gallus Domesticus 540 parts, Radix Salviae Miltiorrhizae 108 parts, Radix Rehmanniae 216 parts, Rhizoma Cyperi (processed with vinegar) 108 parts, Radix Ginseng 108 parts, the Radix Paeoniae Alba 108 parts, Concha Ostreae (calcined) 40 parts, Cornu Cervi Degelatinatum 40 parts, Radix Stellariae 22 parts, 27 parts, Radix Glycyrrhizae, the Radix Astragali 27 parts, Carapax Trionycis (processed) 54 parts;
2) Gallus Domesticus of getting removing feather claw and intestine is cut into block, adds 3-4 times of water gaging, boils 1.5-3.5 hour, use 10: 1 hard paraffin defats while hot, the papain adding 100-400: 1 afterwards stirs, and adjust pH is to 5-6,40-65 DEG C, is hydrolyzed after 3-7 hour, boils 5-15 minute, centrifugal, supernatant is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
3) get Carapax Trionycis (processed), decoct with water 1-3 time, the 2-5 that at every turn adds water doubly, decocts 0.5-4 hour, and filter, merging filtrate, is evaporated to thick paste, and relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, gets dry extract, for subsequent use;
4) broken Radix Ginseng is got, the Radix Paeoniae Alba, Radix Glycyrrhizae decocts with water 2 times, the 3-6 that at every turn adds water doubly, decoct 1.5-2.5 hour, filter, merging filtrate, filtrate or by filtrate reduced in volume to relative density 50 DEG C survey 1.02-1.20, add processed good macroporous adsorbent resin, every gram of medical material 1.5-2.5 gram of resin, with 4-8 times amount water elution, discard water lotion, use 60%-90% ethanol elution again, react to eluent inspection without saponin, eluent decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.34, at 60-90 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use,
5) get broken Radix Salviae Miltiorrhizae, the Radix Astragali and Radix Stellariae, add the ethanol of 60%-80%, reflux, extract, 1-3 time, add ethanol 2-8 times amount at every turn, backflow 0.5-3 hour, filters, merging filtrate, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 50-100 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
6) get broken Radix Rehmanniae, Cornu Cervi Degelatinatum and Concha Ostreae (calcined) and decoct with water 2 times, the 7-9 that at every turn adds water doubly, decocts 1.5-3.5 hour, filter, merging filtrate, concentrating under reduced pressure surveys the thick paste of 1.02-1.35 to relative density 60 DEG C, add 80%-90% ethanol to stir, make alcohol content be 60%-70%, hold over night, get supernatant, filter, decompression recycling ethanol is to thick paste, relative density 60 DEG C surveys 1.02-1.32, at 60-80 DEG C, 0.02-0.12Mpa vacuum drying, get dry extract, for subsequent use;
7) get Rhizoma Cyperi (processed with vinegar) and add 4-8 times of water gaging, be heated to boil, extract volatile oil to oily defecation, volatile oil is for subsequent use;
8) by each for above-mentioned gained dry extract, fine powder, adjuvant 2.1-18% mixed powder is broken into fine powder, sieves; Spray into Rhizoma Cyperi volatile oil, mixing both obtained WUJI BAIFENG Chinese medicine compositions.
7. the preparation method as described in claim 5 or 6, is characterized in that, the macroporous adsorbent resin described in step 4 is selected from the one in D101 or AB-8.
8. the preparation containing any one of claim 1-4 Chinese medicine composition, is characterized in that: the Chinese medicine composition described in preparation is 1-3: 3-1 with adjuvant ratio.
9. Chinese medicinal composition preparation as claimed in claim 8, it is characterized in that, said preparation is tablet, granule or capsule.
10. the application of compositions as claimed in claim 1 in preparation treatment antiinflammatory or spasmolytic medicine.
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| CN114366783A (en) * | 2021-12-06 | 2022-04-19 | 金鸿药业股份有限公司 | Black-bone chicken peptide composition and preparation method and application thereof |
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| CN1457848A (en) * | 2003-06-11 | 2003-11-26 | 天津中新药业集团股份有限公司乐仁堂制药厂 | Black-bone chicken white phoenix preparation medicine and its preparing method |
| CN1723954A (en) * | 2005-07-06 | 2006-01-25 | 北京正大绿洲医药科技有限公司 | Dripping pills made from black-bone chicken, and its prepn. method |
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| CN1457848A (en) * | 2003-06-11 | 2003-11-26 | 天津中新药业集团股份有限公司乐仁堂制药厂 | Black-bone chicken white phoenix preparation medicine and its preparing method |
| CN1723954A (en) * | 2005-07-06 | 2006-01-25 | 北京正大绿洲医药科技有限公司 | Dripping pills made from black-bone chicken, and its prepn. method |
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| CN114366783A (en) * | 2021-12-06 | 2022-04-19 | 金鸿药业股份有限公司 | Black-bone chicken peptide composition and preparation method and application thereof |
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