CN104737666A - Current-year seedling emergence processing method of viburnum sargenti seeds - Google Patents
Current-year seedling emergence processing method of viburnum sargenti seeds Download PDFInfo
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Abstract
The invention relates to a current-year seedling emergence processing method of viburnum sargenti seeds. The method comprises the following steps that before sowing, 1 part of a seed and 3 parts of wet river sands which are soaked with clean water for one week are uniformly mixed and put into a container with high air permeability to provide sand storage lamination; high-temperature germination is carried out at the temperature of 25 DEG C; the wetting sands are kept everyday, and the sands are turned up and down for 2-3 times per day and ventilated; about for two months, the roots of the seeds are germinated to budlets which are called budding; the viburnum sargenti seeds which grow out of budding are washed clean by using water and poured into the container; the obtained preparation is poured into the container to spill over the seeds, and the mixture is soaked for 24 hours; the seeds are fished out after 24 hours so that the sowing can be carried out. According to the current-year seedling emergence processing method of the viburnum sargenti seeds, the epicotyl dormancy can be quickly broken, current-year normal seedling can be carried out after spring sowing, and the epicotyl dormancy is broken without needing reserved bed winter stay or low temperature lamination; moreover, the emergence of the seedlings is ordered, and the emergence rate is 90 percent at most and far more than 40-52 percent of the emergence rate of the second-year emergence seedlings which are obtained by normal sowing, and the emergence rate under the condition of breaking dormancy at low temperature is up to 87 percent.
Description
Technical field
The present invention relates to the processing method utilizing a kind of chicken mock Jia Opulus seed to emerge then.
Background technology
Existing to chicken mock pod Opulus seed processing technology shortcoming: 1 seed seedling-raising time is long, sows and can not emerge then after vernalization, need through staying a winter of bed, Second Year just can be emerged, and emergence rate is low by about 50%; 2 to abolish top-wall effect technical role remarkable, need through high temperature 25 DEG C of laminations 2 months, and abolish a dormancy, low temperature 4 DEG C of laminations abolish top-wall effect in one month, and emergence rate is very low.
Heilongjiang Province indigenous tree species chicken mock Jia Opulus (being also Tianmu lake) (viburnumsargenti), shrub, height about 2-3 rice.Its tree crown, leaf, flower appearance and fruit all very tool sight, see the green great Ye of its palm shape in spring, summer and various have large-scale infertile property radiation flower, graceful grand; Autumn, winter are seen its cherry innumerable place and deposit fruit and Bai Xue phase contrast, and it is scarlet to meet the eye on every side Bai Xuenei, and scenery is charming; Palmate trifoliolate leaves that autumn has set in becomes beautiful redness, and damage by disease and insect is few.But it is less that excellent flowering shrub tree like this plants consumption in city trees and shrubs application is planted, and one of key reason is its seed germination difficulty, and sowing needs to emerge for 2 years and emergence rate is very low.
The most seed of Viburnum has top-wall effect characteristic to have much research unanimously to think at present, and after namely the seed degree of depth or the dormancy of either shallow physiology and appearance are broken, radicle is sprouted but epicotyl is not sprouted.This top-wall effect, also makes Secondary dormancy be the ancient feature that species are not evolved during evolution completely.Therefore this dormancy type abolish extremely difficult.
Chicken mock Jia Opulus seed just belongs to this deep-sleep type, the present research to chicken mock Jia Opulus Germination Characteristics is from depth & wideth aspect all very backwardness, the treatment technology of seed germination only rests on intermittent warming, the research report effectively breaking top-wall effect dormant technology method is not less, and imperfection, concrete.Current seeding and seedling raising present situation is: study clear and definite, utilize high temperature treatment can remove its dormancy, seed can normally germinate, and after sowing in spring, seed coat does not come off, cotyledon can not launch, nursery stock does not grow then, through staying bed winter low temperature vernalization in 1 year, just need can break top-wall effect, Second Year could normally to be emerged growth spring, due to overlong time, suslik, winged insect sting food or plant exhausting of embryo nutrition cause nursery stock to lose in a large number and emerge uneven, Second Year emergence rate is at about 38-50%.Also there is research after high temperature makes chicken mock Jia Opulus sprout, utilize low temperature to break top-wall effect, can realize after planting emerging then, but through us experiment and seedling nursery personnel reflection, utilize the method really can realize emerging then, but emergence rate is extremely low, is less than 3%.Utilize cuttage now, the research that Jia Opulus nursery stock is bred in group training is also a lot, and the cuttage technique of chicken mock Jia Opulus is also comparatively ripe, but the source of cuttage material is more and more difficult, therefore really cannot realize the industrialization of nursery.Tissue culture is especially because condition of culture requires that harshness also cannot be applied to actual production.Chicken mock Jia Opulus seeds result quantities is large, seed production is high, maturity good, therefore seed seedling-raising is the important method solving its market nurseries' supplies eventually, and economical and practical.
Summary of the invention: the object of the present invention is to provide the processing method that a kind of chicken mock Jia Opulus seed is emerged then, chicken mock Jia Opulus seed is made to break the top-wall effect characteristic that it hinders sprouting within a short period of time, after planting just can normally emerge then, and it is neat to emerge, and emergence rate is up to 90%.Successful is better than processing method conventional at present.
Technical scheme of the present invention is: the processing method that a kind of chicken mock Jia Opulus seed is emerged then, it is characterized in that: (1) its raw material is gibberellin (English name: gibberellin English abbreviation: GA
3, chemical formula C
19h
22o
6) and absolute alcohol, clear water, (2) preparation method: by ratio of quality and the number of copies be by gibberellin and water two kinds of materials: 1:10000 mixes, and namely 100mg gibberellin adds in 1000000mg water, mixing, is fully dissolved by gibberellin before mixing with trace (1ppm) absolute alcohol, (3) concrete implementation step: before sowing, clear water is soaked 1 part, the seed of a week, the ratio of moistening river sand 3 parts mixes, be placed in the container of good air permeability and hide lamination for husky, high temperature vernalization is carried out under 25 DEG C of temperature condition, keep every day sand moistening, stir ventilative up and down one day 2-3 time, about 2 months, seed root sprouts budlet for sprouting, pour in container after the chicken mock Jia Opulus seed water sprouted is cleaned up, pour the preparation that step (2) obtains into container and do not have seed, soak 24 hours, pull seed after 24 hours out can sow.
1ppm equals 1mg solute and is dissolved in 1000000mg solvent, and 100ppm equals 100mg solute and is dissolved in 1000000mg solvent.Because gibberellin is water insoluble, must dissolve through alcohol could be water-soluble, and alcohol is only responsible for dissolving gibberellin, and it is also not counted in proportion.
The present invention not only eliminates chicken mock Jia Opulus seed and has to pass through cold stratification abolishes top-wall effect this step over 1 month; And no longer need chicken mock Jia Opulus after planting bed must stayed to pass the winter, solve this key issue just can emerged next year, this avoid suslik during staying bed, insect sting food, plant embryo nutrition exhaust and Baconic to freeze to death etc. and causes nursery stock to lose in a large number, and this disadvantage uneven of emerging.Fundamentally solve the problem of chicken mock Jia Opulus seed seedling-raising difficulty.
Specific experiment method:
1. test material: the chicken mock Jia Opulus seed germinateed
2. test method
Variable concentrations gibberellin is utilized to carry out the process of different time to chitting piece.Often organize experiment get 250g, if 50,100,150, a 200ppm4 concentration, each concentration all arranges 4 immersion treatment times, is respectively 1,5,10,24 hour.Low temperature treatment is: at 0 DEG C ,-5 DEG C ,-10 DEG C temperature, carry out sand at low temperature after seed sprouting hide lamination 7 days, 14 days, 21 days, often group experiment processes three samples simultaneously, after process, 1000 planting seeds got by each sample, May is sowed on seedbed, seedling looks into emergence rate after all sprouting then, the emergence rate of three samples is added gets its mean value, is the percentage of emerging then that this group is tested.Detailed results as shown in Figure 5.
3. result
The most seed of Jia Opulus has top-wall effect characteristic, and after namely the seed degree of depth or the dormancy of either shallow physiology and appearance are broken, radicle is sprouted but epicotyl is not sprouted.This top-wall effect is the ancient feature that species are not evolved during evolution completely.Therefore this dormancy type abolish extremely difficult.A lot of research shows that chicken mock Jia Opulus just has top-wall effect characteristic, after planting needs could normally emerge through a next year in winter.Therefore we utilize gibberellin and low temperature treatment to carry out abolishing the experiment of chicken mock Jia Opulus top-wall effect, and result as shown in Figure 5.
Experiment finds, utilize the chicken mock Jia Opulus seed that sprouts of variable concentrations process, spring after planting can normally emerge.Chicken mock Jia Opulus low concentration 50ppm, along with the increase in processing time, after planting emergence rate also increases, and within 24 hours, leaf emergence is 74%.When concentration is 150ppm, is increase with the lengthening emergence rate of soak time yet yet, but soaks 24 hours, its emergence rate does not have 100ppm concentration and soaks that 24 hours high but gap is little.Concentration the highest 200ppm time processing time short emergence rate also can reach good effect, the time is increased to do not have process for 24 hours on the contrary for 10 hours effective, and the process of 10 hours can reach the emergence rate of maximum 90%.What treatment effect was best soaks 4 hours for concentration 100ppm2, and concentration 200ppm soaks 10 hours, and emergence rate can reach 90% then.
There are some researches show and to carry out after sprouting freezingly can abolishing top-wall effect characteristic, can emerge then, therefore we have selected the cool stratification that 0--10 DEG C of several thermograde carries out 7-21 days, found that chicken mock Jia Opulus whole browning of radicle at lower than the temperature of 0 DEG C is freezing, cannot emerge.It is husky at 0 DEG C of temperature that to hide 21 days germinated seeds also all intact without frostbite, but then emergence rate all lower than 3%.
By comparing utilize gibberellin to break emergence rate that chicken mock top-wall effect concentration 100ppm soaks 24 hours and 200ppm, to soak 10 hours emerge identical, but concentration 100ppm is more economical and practical during a large amount of nursery in nursery, therefore selects gibberellin 100ppm to soak and be treated to the best approach in 24 hours.
The invention provides the reasonable formula for a product of the one prepared by auxin, in addition simple immersion process, reach and make chicken mock Jia Opulus seed after 25 DEG C of conventional husky Tibetan laminations are germinateed, its top-wall effect can be abolished fast, can normally emerge then after spring sowing, no longer need to stay bed to pass the winter or cool stratification to abolish top-wall effect, and it is neat to emerge, emergence rate is up to 90%, far away higher than the emergence rate 40-52% that normal sowing Second Year is emerged, and the emergence rate 87% of low temperature regime Dormancy breaking.
Accompanying drawing explanation
Fig. 1 is directly sowing after original technology chicken mock Jia Opulus germinates.
Fig. 2 is through this method process, after planting just normally can slough seed coat then, and normal growth takes off seed coat situation.
Fig. 3 is for after utilizing this method process, and chicken mock Jia Opulus seed seedling dish is after planting emerged situation then.
Fig. 4 is for after utilizing this method process, and chicken mock Jia Opulus seeding in nursery bed is emerged situation then.
To be gibberellin and low temperature abolish top-wall effect to chicken mock Jia Opulus seed to Fig. 5 affects situation.
Embodiment:
The processing method that chicken mock Jia Opulus seed is emerged then, (1) its raw material is gibberellin (English name: gibberellin English abbreviation: GA
3, chemical formula C
19h
22o
6) and absolute alcohol, clear water, (2) preparation method: by ratio of quality and the number of copies be by gibberellin and water two kinds of materials: 1:10000 mixes, and namely 100mg gibberellin adds in 1000000mg water, mixing, is fully dissolved by gibberellin before mixing with trace (1ppm) absolute alcohol, (3) concrete implementation step: before sowing, clear water is soaked 1 part, the seed of a week, the ratio of moistening river sand 3 parts mixes, be placed in the container of good air permeability and hide lamination for husky, high temperature vernalization is carried out under 25 DEG C of temperature condition, keep every day sand moistening, stir ventilative up and down one day 2-3 time, about 2 months, seed root sprouts budlet for sprouting, pour in container after the chicken mock Jia Opulus seed water sprouted is cleaned up, pour the preparation that step (2) obtains into container and do not have seed, soak 24 hours, pull seed after 24 hours out can sow.
Chicken mock Jia Opulus directly sows after germinateing as shown in Figure 1, and not de-seed coat after being unearthed, cannot normal growth situation.As shown in Figure 2 through this method process, after planting just normally can slough seed coat then, normal growth, figure is de-seed coat situation.After utilizing this method process as shown in Figure 3, chicken mock Jia Opulus seed seedling dish after planting, situation of emerging then.After utilizing this method process as shown in Figure 4, chicken mock Jia Opulus seeding in nursery bed is worked as, situation of emerging in year.
Claims (1)
1. a chicken mock pod Opulus seed processing method of emerging then, is characterized in that: (1) its raw material is gibberellin (English name: gibberellin English abbreviation: GA
3, chemical formula C
19h
22o
6) and absolute alcohol, clear water, (2) preparation method: by ratio of quality and the number of copies be by gibberellin and water two kinds of materials: 1:10000 mixes, and namely 100mg gibberellin adds in 1000000mg water, mixing, is fully dissolved by gibberellin before mixing with trace (1ppm) absolute alcohol, (3) concrete implementation step: before sowing, clear water is soaked 1 part, the seed of a week, the ratio of moistening river sand 3 parts mixes, be placed in the container of good air permeability and hide lamination for husky, high temperature vernalization is carried out under 25 DEG C of temperature condition, keep every day sand moistening, stir ventilative up and down one day 2-3 time, about 2 months, seed root sprouts budlet for sprouting, pour in container after the chicken mock Jia Opulus seed water sprouted is cleaned up, pour the preparation that step (2) obtains into container and do not have seed, soak 24 hours, pull seed after 24 hours out can sow.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605616A (en) * | 2016-12-09 | 2018-10-02 | 宁夏农林科学院固原分院 | Poly- Hua Jia Opulus gently simplify method for culturing seedlings |
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2015
- 2015-03-13 CN CN201510109649.1A patent/CN104737666A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 万书成: "鸡树条荚蒾种子变温催芽处理的研究", 《育苗技术》 * |
| 杨轶华等: "4种荚蒾种子萌发特性研究", 《国土与自然资源研究》 * |
| 杨轶华等: "赤霉素破除鸡树条荚蒾种子双休眠特性技术初探", 《国土与自然资源研究》 * |
| 高玉艳等: "鸡树条荚蒾种子休眠的试验初报", 《中国林副特产》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605616A (en) * | 2016-12-09 | 2018-10-02 | 宁夏农林科学院固原分院 | Poly- Hua Jia Opulus gently simplify method for culturing seedlings |
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Application publication date: 20150701 |