CN104736696B - 用于产生棒麦角素型生物碱的基因和方法 - Google Patents

用于产生棒麦角素型生物碱的基因和方法 Download PDF

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CN104736696B
CN104736696B CN201380054217.4A CN201380054217A CN104736696B CN 104736696 B CN104736696 B CN 104736696B CN 201380054217 A CN201380054217 A CN 201380054217A CN 104736696 B CN104736696 B CN 104736696B
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polypeptide
seq
amino acid
activity
recombinant
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CN104736696A (zh
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M·尼斯比
C·福利
C·A·F·尼尔森
A·哈驰
M·施瓦布
O·策尔德尔
S·哈夫纳
H·施罗德
B·霍夫
A·莫尔特
K·迪特布里希
M·布罗伊尔
H·哈特曼
K·科尔博尔
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Iowa Co ltd
BASF SE
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BASF SE
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CN201380054217.4A 2012-08-22 2013-08-13 用于产生棒麦角素型生物碱的基因和方法 Expired - Fee Related CN104736696B (zh)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201261691848P 2012-08-22 2012-08-22
US61/691848 2012-08-22
EP12181388.5 2012-08-22
EP12181388 2012-08-22
US201361786841P 2013-03-15 2013-03-15
EP13159444 2013-03-15
EP13159444.2 2013-03-15
US61/786841 2013-03-15
EP13178008.2 2013-07-25
EP13178008 2013-07-25
PCT/IB2013/056604 WO2014030096A2 (en) 2012-08-22 2013-08-13 Genes and processes for the production of clavine-type alkaloids

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Families Citing this family (11)

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Publication number Priority date Publication date Assignee Title
EP3080273A4 (en) 2013-12-13 2017-11-08 Basf Se Recombinant microorganism for improved production of fine chemicals
US9790528B2 (en) * 2014-06-16 2017-10-17 West Virginia University Production of lysergic acid by genetic modification of a fungus
US10428361B2 (en) 2015-03-26 2019-10-01 Basf Se Biocatalytic production of l-fucose
WO2019183193A1 (en) * 2018-03-20 2019-09-26 Manus Bio, Inc. Enzymes, cells, and methods for production of 3-(4-farnesyloxyphenyl)propionic acid
EP3714959A1 (en) * 2019-03-29 2020-09-30 Basf Se Isolation of small molecules from the fermentation broth of a eukaryotic microorganism
CZ308933B6 (cs) * 2019-07-19 2021-09-15 Univerzita Palackého v Olomouci Houba Claviceps purpurea se zvýšenou produkcí námelových alkaloidů, způsob její přípravy, expresní kazeta
CN113308442B (zh) * 2021-04-28 2023-06-27 天津大学 重组酿酒酵母菌株及其构建方法
CN113174399A (zh) * 2021-04-28 2021-07-27 天津大学 重组酿酒酵母菌株及其发酵方法
CN115948442A (zh) * 2022-08-04 2023-04-11 杭州佳嘉乐生物技术有限公司 一种提高植物发酵原浆中生物碱含量的方法
WO2024064811A2 (en) * 2022-09-22 2024-03-28 Tryptagenix, Inc. Polynucleotides, polypeptides, recombinant cells and methods for generating ergolines and precursors and metabolites thereof
CN116103351B (zh) * 2023-03-06 2025-06-10 天津大学 高产田麦角碱重组酵母构建方法与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101238208A (zh) * 2005-11-07 2008-08-06 伊瓦克斯制药有限公司 真菌麦角菌（Fr.)Tul.的工业生产菌株

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US7192751B2 (en) 2001-12-06 2007-03-20 The Regents Of The University Of California Biosynthesis of amorpha-4,11-diene
CA2825979A1 (en) * 2011-03-03 2012-09-07 Basf Se Gene cluster for biosynthesis of cycloclavine

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101238208A (zh) * 2005-11-07 2008-08-06 伊瓦克斯制药有限公司 真菌麦角菌（Fr.)Tul.的工业生产菌株

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Comparision of Ergot Alkaloid Biosynthesis Gene Clusters in Claviceps Species Indicates Loss of Late Pathway Steps in Evolution of C.fusiformis.;Nicole Lorenz等;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;20071130;第73卷(第22期);结果与讨论部分 *
Ergot alkaloids:structure diversity,biosynthetic gene clusters and funtional proof of biosynthetic genes;Christiane Wallwey等;《Nat.Prod.Rep.》;20101224;第28卷;496-510 *
New routes to clavine-type ergot alkaloids. Part 2: Synthesis of the last,so far not yet synthesized member of the clavine alkaloid family,(±)-cycloclavine;Maria Incze;《Tetrahedron》;20080131;第64卷;2924-2929 *
The ergot alkaloid gene cluster: Functional analyses and evolutionary aspects;Nicole Lorenz等;《Phytochemistry》;20090818;第79卷;1822-1832 *
Towards Elimination And Genetic Maniqulation Of Ergot Alkaloid Production In Fungal Endophytes.;Simona Florea等;《University of Kentucky Doctoral Dissertations》;20091231;第4-5章、图4.1-4.3、表4.1 *

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