CN104704112A - 传染性流感病毒的生产 - Google Patents

传染性流感病毒的生产 Download PDF

Info

Publication number
CN104704112A
CN104704112A CN201380051942.6A CN201380051942A CN104704112A CN 104704112 A CN104704112 A CN 104704112A CN 201380051942 A CN201380051942 A CN 201380051942A CN 104704112 A CN104704112 A CN 104704112A
Authority
CN
China
Prior art keywords
virus
influenza
cells
promoter
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380051942.6A
Other languages
English (en)
Chinese (zh)
Inventor
I·勒加斯特洛伊斯
J·麦迪纳
C·莫斯特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Inc
Original Assignee
Sanofi Pasteur Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Pasteur Inc filed Critical Sanofi Pasteur Inc
Publication of CN104704112A publication Critical patent/CN104704112A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/00051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/00051—Methods of production or purification of viral material
    • C12N2760/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16161—Methods of inactivation or attenuation
    • C12N2760/16164—Methods of inactivation or attenuation by serial passage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16261—Methods of inactivation or attenuation
    • C12N2760/16264—Methods of inactivation or attenuation by serial passage

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380051942.6A 2012-08-03 2013-07-29 传染性流感病毒的生产 Pending CN104704112A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12305968.5 2012-08-03
EP12305968 2012-08-03
PCT/EP2013/065920 WO2014019990A1 (en) 2012-08-03 2013-07-29 Production of infectious influenza viruses

Publications (1)

Publication Number Publication Date
CN104704112A true CN104704112A (zh) 2015-06-10

Family

ID=48900986

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380051942.6A Pending CN104704112A (zh) 2012-08-03 2013-07-29 传染性流感病毒的生产

Country Status (11)

Country Link
US (1) US9982240B2 (enExample)
EP (1) EP2880154B1 (enExample)
JP (1) JP6335167B2 (enExample)
KR (1) KR20150038374A (enExample)
CN (1) CN104704112A (enExample)
AU (1) AU2013298632B2 (enExample)
BR (1) BR112015002268A2 (enExample)
CA (1) CA2880409A1 (enExample)
MX (1) MX360120B (enExample)
SG (1) SG11201500508XA (enExample)
WO (1) WO2014019990A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105754997A (zh) * 2016-03-30 2016-07-13 华中农业大学 狂犬病毒drv-ah08株的先导rna及其在制备预防与治疗狂犬病毒的药品中的应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112015030582A2 (pt) * 2013-06-06 2017-08-29 Novartis Ag Segmento de hemaglutinina da gripe quimérico e de neuraminidase quimérico, proteína de hemaglutinina quimérica, vírus da gripe rearranjado, métodos para preparação de um vírus da gripe rearranjado e para preparação de uma vacina e sistema de expressão
CN104232594B (zh) * 2014-09-11 2017-05-31 中国农业科学院哈尔滨兽医研究所 重组类禽型h1n1流感病毒灭活疫苗株(js40/pr8)及其制备方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1970081A (zh) * 2006-11-09 2007-05-30 中国农业科学院哈尔滨兽医研究所 禽流感病毒标记疫苗及其制备方法和应用
WO2009152181A1 (en) * 2008-06-11 2009-12-17 Flugen, Inc. Cell-based systems for producing influenza vaccines
US20120058538A1 (en) * 1998-09-14 2012-03-08 Peter Palese Helper-free Rescue of Recombinant Negative Strand RNA Virus Systems

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1445322B2 (en) 1995-06-15 2012-06-06 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
TW389999B (en) 1995-11-21 2000-05-11 Toshiba Corp Substrate having shallow trench isolation and method of manufacturing the same
US8715940B2 (en) 1999-04-06 2014-05-06 Wisconsin Alumni Research Foundation Method of making recombinant influenza virus
GB9916794D0 (en) * 1999-07-16 1999-09-22 Isis Innovation In vitro virus reconstitution
GB9923176D0 (en) 1999-09-30 1999-12-01 Smithkline Beecham Biolog Novel composition
DE60137345D1 (de) 2000-04-28 2009-02-26 St Jude Childrens Res Hospital Dna-transfektionssystem zur erzeugung von infektiösen negativsträngigen rna virus
ES2230502T3 (es) 2001-04-23 2005-05-01 Amaxa Gmbh Disolucion tampon para la electroporacion y procedimiento que incluye el uso de la misma.
US20050158342A1 (en) 2003-12-23 2005-07-21 Medimmune, Inc. Multi plasmid system for the production of influenza virus
US20080254065A1 (en) * 2004-03-09 2008-10-16 Chiron Corporation Influenza Virus Vaccines
EP1741778B1 (de) 2005-07-07 2007-12-26 Amaxa AG Verfahren zur Behandlung geringer Volumina mit elektrischem Strom
US20070111309A1 (en) 2005-10-04 2007-05-17 Daelli Marcelo G Vero cell line adapted to grow in suspension
DK2307538T3 (da) 2008-07-15 2016-01-18 Crucell Holland Bv Skalérbar fremgangsmåde til dyrkning af per.c6-celler og fremstilling af produkter derfra

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120058538A1 (en) * 1998-09-14 2012-03-08 Peter Palese Helper-free Rescue of Recombinant Negative Strand RNA Virus Systems
CN1970081A (zh) * 2006-11-09 2007-05-30 中国农业科学院哈尔滨兽医研究所 禽流感病毒标记疫苗及其制备方法和应用
WO2009152181A1 (en) * 2008-06-11 2009-12-17 Flugen, Inc. Cell-based systems for producing influenza vaccines

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
EMMIE DE WIT等: "A reverse-genetics system for Influenza A virus using T7 RNA polymerase", 《JOURNAL OF GENERAL VIROLOGY》 *
ERICH HOFFMANN等: "A DNA transfection system for generation of influenza A virus from eight plasmids", 《PNAS》 *
ERVIN FODOR等: "Rescue of Influenza A Virus from Recombinant DNA", 《JOURNAL OF VIROLOGY》 *
ISABELLE LEGASTELOIS等: "Preparation of genetically engineered A/H5N1 and A/H7N1 pandemic vaccine viruses by reverse genetics in a mixture of Vero and chicken embryo cells", 《INFLUENZA AND OTHER RESPIRATORY VIRUSES》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105754997A (zh) * 2016-03-30 2016-07-13 华中农业大学 狂犬病毒drv-ah08株的先导rna及其在制备预防与治疗狂犬病毒的药品中的应用
CN105754997B (zh) * 2016-03-30 2019-02-26 华中农业大学 狂犬病毒drv-ah08株的先导rna及其在制备预防与治疗狂犬病毒的药品中的应用

Also Published As

Publication number Publication date
AU2013298632A1 (en) 2015-02-26
JP2015530875A (ja) 2015-10-29
EP2880154B1 (en) 2019-05-15
AU2013298632B2 (en) 2018-11-01
BR112015002268A2 (pt) 2017-11-07
SG11201500508XA (en) 2015-02-27
US20150191703A1 (en) 2015-07-09
JP6335167B2 (ja) 2018-05-30
MX2015001499A (es) 2015-08-20
US9982240B2 (en) 2018-05-29
EP2880154A1 (en) 2015-06-10
KR20150038374A (ko) 2015-04-08
CA2880409A1 (en) 2014-02-06
WO2014019990A1 (en) 2014-02-06
MX360120B (es) 2018-10-23

Similar Documents

Publication Publication Date Title
JP6352974B2 (ja) ワクチンのための高力価組み換えインフルエンザ・ウィルス
CN1826407B (zh) 用于疫苗和基因治疗的高滴度重组流感病毒
US10358630B2 (en) Recombinant influenza vectors with tandem transcription units
EP2493912B1 (en) High titer recombinant influenza viruses with enhanced replication in vero cells
KR101272487B1 (ko) 인플루엔자 바이러스의 구제
US8883995B2 (en) Live attenuated influenza virus vaccines comprising microRNA response elements
US8475807B2 (en) Avian influenza virus live attenuated vaccine and uses thereof
JP5491173B2 (ja) イヌ細胞中でマイナス鎖ウイルスｒｎａを発現させるための方法および組成物
US10494613B2 (en) Generation of infectious influenza viruses from virus-like particles
US20090324640A1 (en) Neuraminidase-deficient live influenza vaccines
US9982240B2 (en) Production of infectious influenza viruses
JP5762969B2 (ja) ｐＨ安定性のエンベロープ付きウィルスの生産方法
US20230310575A1 (en) Hemagglutinin Modifications for Improved Influenza Vaccine Production
WO2010046335A1 (en) Production of influenza virus by reverse genetics in per.c6 cells under serum free conditions
HK1082769B (en) High titer recombinant influenza viruses for vaccines and gene therapy

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
AD01 Patent right deemed abandoned
AD01 Patent right deemed abandoned

Effective date of abandoning: 20200110