CN104686784A - Technology for preparing feed through glutamic acid waste liquid - Google Patents
Technology for preparing feed through glutamic acid waste liquid Download PDFInfo
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- CN104686784A CN104686784A CN201510168334.4A CN201510168334A CN104686784A CN 104686784 A CN104686784 A CN 104686784A CN 201510168334 A CN201510168334 A CN 201510168334A CN 104686784 A CN104686784 A CN 104686784A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention belongs to the field of fermentation waste liquid treatment in the biological fermentation industry, and discloses a technology for preparing feed through glutamic acid waste liquid. The technology comprises the following steps that 1, a glutamic acid crystal and the waste liquid are prepared; 2, egg white is finely prepared; 3, the chicken feed is prepared; 4, compound fertilizer is prepared. The feed prepared through the method is good in breeding effect, waste is turned into wealth, and huge economic benefits can be brought to enterprises.
Description
Technical field
The invention belongs to biofermentation industry Wastewater treating field, be specifically related to a kind of technique utilizing waste water of mgs to prepare feed.
Background technology
Glutamic acid is a kind of acidic amino acid.Molecule includes two carboxyls, and chemical name is alpha-amido glutaric acid.Glutamic acid is that inner Suo Xun finds for 1856, and be clear crystal, have delicate flavour, be present in grain protein in a large number, in animal brain, content is also more.Account for critical role in glutamic acid protein metabolism process in vivo, participate in the many important chemical reaction in animal, plant and microorganism.Sodium glutamate is commonly called as monosodium glutamate, is important tasty agents, has humidification to fragrance.Sodium glutamate is widely used in food flavor, both can be used alone, and can use with other amino acid etc. again.For in food, there is flavouring effect.Along with the development of amino acid fermentation technology, China has become the production and consumption big country of monosodium glutamate, but the wastewater flow rate discharged in glutamate production process is large, the mother liquor that fermented gournet powder liquid discharges after waiting electricity to extract glutamic acid has that CODCr is high, BOD5 is high, thalline content is high, content is high, ammonia-nitrogen content is high and the feature of pH value low " five high low " for sulfate radical (use instead sulfuric acid adjust pH before be chlorion).It is the industrial wastewater that a kind of difficulty of governance is very large.Owing to effectively can not administer gourmet powder waste water, many Gourmet Powder Factories are put into the row of national major polluting sources unit, and the improvement of gourmet powder waste water has become the great difficult problem of restriction glutamate production enterprise development.The a large amount of thalline contained in waste water, it is a kind of single cell protein, containing rich in protein.
Along with improving constantly of cost of material, livestock feed price rises steadily, and makes the benefit of raiser year after year low, causes aquaculture very risky, dampened the enthusiasm of numerous raisers significantly.Current full price commercial feed is mostly be that master is equipped with various auxin again, hormone is formed by combining with fine fodder.The medicament residue such as antibiotic, growth hormone in pig feed, makes the quality of pork and mouthfeel significantly decline, and can not meet again the requirement of people to healthy diet.
Chinese invention patent CN201410540679 " utilizes animal feed prepared by glutamic acid fermentation waste material " and discloses a kind of animal feed utilizing glutamic acid fermentation waste material to prepare, and this feed makes full use of discarded object and prepared feed; But various waste material does not process by respectively, does not utilize added value to greatest extent, and utilize waste water also not expected on the long-term impact of animal to prepare feed.How effectively utilizing glutamic acid-fermented waste liquid, improve the added value of industry to greatest extent, is the direction that enterprise is pursued always.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of technique utilizing waste water of mgs to prepare feed, the method has prepared high purity protein, feed and Chemical Mixed Fertilizer simultaneously, effectively make use of component of effluent, overall process produces without discarded object, environment friendly and pollution-free, the added value of industry is high, brings rich profit to enterprise.
For achieving the above object, the present invention is by the following technical solutions:
Utilize waste water of mgs to prepare a technique for feed, it comprises the steps: that step 1) prepares glutamic acid crystal and waste liquid, step 2) purified protein, step 3) prepares chicken feed, and step 4) prepares Chemical Mixed Fertilizer.
Particularly, described technique comprises the steps:
Step 1) prepares glutamic acid crystal and waste liquid: glutami acid fermentation liquor is the filter of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrate and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation glutamic acid crystal and mother liquor; Merge above-mentioned trapped substance, concentrate and mother liquor, obtain waste liquid;
Step 2) purified protein: waste liquid is heated, makes temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 10min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying chambers to dry to constant weight, Ultraviolet radiation 5min subsequently, uitraviolet intensity is 2000uW/cm
2; Then thalline is added in stirred reactor, and add the hydrochloric acid solution of 1M, 100 turns/min stirring reaction 15min, cell membrane is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant, be condensed into paste, finally by slurry-spraying pelletizing fluidized bed drying, obtain albumen; Wherein, the mass ratio of thalline and hydrochloric acid solution is 1:2;
Step 3) prepares chicken feed: by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell membrane merges, and natural subsidence Separation of Solid and Liquid, obtains supernatant and sediment; Sediment, wheat bran, fishbone dust and the rice chaff mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment moisture content to 50% mass fraction, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of aeration-dryings, pulverize and obtain chicken feed;
Step 4) prepares Chemical Mixed Fertilizer: in step 3) gained supernatant, add humic acid potassium, diatomite and rice straw powder, stir, and leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, MAP, poly-aspartate and the zinc sulfate mass ratio according to 200:10:1:1 is mixed, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging; Wherein, the mass ratio of step 3) gained supernatant, humic acid potassium, diatomite, rice straw powder and ammoniacal liquor is 50:15:12:10:5.
In described step 1), microfiltration membranes is inorganic ceramic membrane, and molecular cut off is 3000Da, and micro-filtration temperature is 37 DEG C; Milipore filter molecular cut off is 300 Da, and ultrafiltrate temperature is 37 DEG C.
The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
Beneficial effect of the present invention mainly comprises:
Because waste water of mgs contains a large amount of fermentation thalli, albumen, sugar and fiber substance, the present invention, while production glutamic acid, make use of waste liquid, eliminate waste treatment process, reduce production cost, achieve and turn waste into wealth, increased substantially the utilization rate of resource; The present invention has prepared high purity protein, feed and Chemical Mixed Fertilizer simultaneously, and effectively make use of waste mother liquor composition, overall process produces without discarded object, environment friendly and pollution-free; The present invention adopts classification processing mode, and the high purity protein added value of industry of preparation is high, and the feed culture efficiency of preparation is good, and the Chemical Mixed Fertilizer fertilizer efficiency of preparation is lasting, with low cost, is applicable to various crop; The present invention, in conjunction with high speed centrifugation, ultraviolet irradiation and acid hydrolysis multiple technologies, substantially increases the cleavage rate of thalline, improves protein yield; Feed of the present invention adopts ammonification and enzyme treatment technique, effectively make use of fermentation waste and agricultural residue, makes feed nutrient more comprehensively; The present invention adopts waste water to prepare fertilizer, avoids waste water resource, and provides trace element and aminoacid ingredient, makes fertilizer nutrient comprehensive and lasting more.
Detailed description of the invention
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, carry out clearly, intactly describing to the present invention, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
Utilize waste water of mgs to prepare a technique for feed, it comprises the steps:
1) prepare glutamic acid crystal and waste liquid: glutami acid fermentation liquor is the filter of 1mm through aperture, collect filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrate and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation glutamic acid crystal and mother liquor; Merge above-mentioned trapped substance, concentrate and mother liquor, obtain waste liquid; Wherein, microfiltration membranes is inorganic ceramic membrane, and molecular cut off is 3000 Da, and micro-filtration temperature is 37 DEG C; Milipore filter molecular cut off is 300 Da, and ultrafiltrate temperature is 37 DEG C;
2) purified protein: waste liquid is heated, makes temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 10min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying chambers to dry to constant weight, Ultraviolet radiation 5min subsequently, thalline thickness is 10cm, and uitraviolet intensity is 2000uW/cm
2; Then thalline is added in stirred reactor, and add the hydrochloric acid solution of 1M, 100 turns/min stirring reaction 15min, cell membrane is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant, be condensed into paste, finally by slurry-spraying pelletizing fluidized bed drying, obtain albumen; Wherein, the mass ratio of thalline and hydrochloric acid solution is 1:2;
3) chicken feed is prepared: by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell membrane merges, and natural subsidence Separation of Solid and Liquid, obtains supernatant and sediment; Sediment, wheat bran, fishbone dust and the rice chaff mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment moisture content to 50% mass fraction, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of aeration-dryings, pulverize and obtain chicken feed;
The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g;
4) prepare Chemical Mixed Fertilizer: in step 3) gained supernatant, add humic acid potassium, diatomite and rice straw powder, stir, leave standstill 12 hours, then add ammoniacal liquor, stir, to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, MAP, poly-aspartate and the zinc sulfate mass ratio according to 200:10:1:1 is mixed, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging; Wherein, the mass ratio of step 3) gained supernatant, humic acid potassium, diatomite, rice straw powder and ammoniacal liquor is 50:15:12:10:5.
Embodiment 2
The albumen that embodiment 1 is prepared by the present invention, purity can reach more than 85%, substitutes the dusty yeast in commercial standard YPD culture medium, all the other components unchanged, cultivate saccharomyces cerevisiae and Pichia pastoris under the same conditions, evaluated the culture effect of product by the growing state (OD600) comparing cell.Specifically in table 1:
Table 1
| Group | Saccharomyces cerevisiae cultivates 20h | Pichia pastoris cultivates 20h |
| Embodiment 1 | 2.33 | 1.60 |
| Commercially available dusty yeast | 1.99 | 1.51 |
Conclusion: utilize turbidimetry for Determination OD
600characterize the growing state of cell, show can substitute commercially available dusty yeast product with this product, culture effect is better, can bring huge economic benefit to enterprise.
Embodiment 3
Feed prepared by embodiment 1 is for the impact of growth and development of chickens
Control group: basal diet: plain edition feed=10:1;
Test group: basal diet: embodiment 1 feed=10:1;
Select healthy chick, totally 40, be divided into two groups at random, comprise control group and test group, test period 40 days.
Weigh respectively at the 1st day and the 40th day, add up the average daily gain (g/ only) of chicken after each group of forage feed, specifically in table 2.
The average daily gain contrast table of table 2 chicken
| Group | 1st day counterpoise (g/ only) | 40th day counterpoise (g/ only) | Average weight gain (g/ only) |
| Control group | 41.3 | 1392.7 | 1351.4 |
| Test group | 40.9 | 1597.8 | 1556.9 |
Conclusion: as shown in Table 2, the chicken average daily gain of test group is greater than control group, illustrates that the speed of growth of the chicken of forage feed of the present invention is significantly faster than the speed of growth of the chicken of plain edition forage feed.
Embodiment 4
Chemical Mixed Fertilizer plantation Ipomoea batatas test prepared by embodiment 1:
Arrange two process experimental plots, area is 10 mu, is control group and experimental group.Control group adopts composite fertilizer (N16-P22-K7), and test group adopts Chemical Mixed Fertilizer of the present invention, and the soil property in experimental plot is identical with planting conditions, and dose is 60kg/ mu.After results, experimental group per mu yield is 2739Kg, and control group per mu yield is 2413Kg, per muly increases extra earning more than hundred yuan.
Although above done detailed explanation with general explanation and detailed description of the invention to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (4)
1. utilize waste water of mgs to prepare a technique for feed, it is characterized in that, described technique comprises the steps: that step 1) prepares glutamic acid crystal and waste liquid, step 2) purified protein, step 3) prepares chicken feed, and step 4) prepares Chemical Mixed Fertilizer.
2. technique according to claim 1, is characterized in that, described technique comprises the steps:
Step 1) prepares glutamic acid crystal and waste liquid: glutami acid fermentation liquor is the filter of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrate and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation glutamic acid crystal and mother liquor; Merge above-mentioned trapped substance, concentrate and mother liquor, obtain waste liquid;
Step 2) purified protein: waste liquid is heated, makes temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 10min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying chambers to dry to constant weight, Ultraviolet radiation 5min subsequently, uitraviolet intensity is 2000uW/cm
2; Then thalline is added in stirred reactor, and add the hydrochloric acid solution of 1M, 100 turns/min stirring reaction 15min, cell membrane is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant, be condensed into paste, finally by slurry-spraying pelletizing fluidized bed drying, obtain albumen; Wherein, the mass ratio of thalline and hydrochloric acid solution is 1:2;
Step 3) prepares chicken feed: by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell membrane merges, and natural subsidence Separation of Solid and Liquid, obtains supernatant and sediment; Sediment, wheat bran, fishbone dust and the rice chaff mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment moisture content to 50% mass fraction, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of aeration-dryings, pulverize and obtain chicken feed;
Step 4) prepares Chemical Mixed Fertilizer: in step 3) gained supernatant, add humic acid potassium, diatomite and rice straw powder, stir, and leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, MAP, poly-aspartate and the zinc sulfate mass ratio according to 200:10:1:1 is mixed, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging; Wherein, the mass ratio of step 3) gained supernatant, humic acid potassium, diatomite, rice straw powder and ammoniacal liquor is 50:15:12:10:5.
3. technique according to claim 2, is characterized in that, in described step 1), microfiltration membranes is inorganic ceramic membrane, and molecular cut off is 3000Da, and micro-filtration temperature is 37 DEG C; Milipore filter molecular cut off is 300Da, and ultrafiltrate temperature is 37 DEG C.
4. the technique according to Claims 2 or 3, is characterized in that, the enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
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| CN106946976A (en) * | 2017-05-19 | 2017-07-14 | 卢松 | Recycle the method that glutamic acid fermentation discards thalline |
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| CN107011409A (en) * | 2017-05-19 | 2017-08-04 | 卢松 | Amino acid fermentation discards the handling process of thalline |
| CN107974473A (en) * | 2017-12-15 | 2018-05-01 | 宁夏伊品生物科技股份有限公司 | The fermenting and producing of glutamic acid and post processing |
| CN108606299A (en) * | 2018-05-08 | 2018-10-02 | 刘柏海 | A method of producing monosodium glutamate using multistage sterilization technique |
| CN110089622A (en) * | 2019-06-19 | 2019-08-06 | 庞红兴 | Aquatic feeds of plant protein source and preparation method thereof |
| CN112047519A (en) * | 2020-09-18 | 2020-12-08 | 安徽华恒生物科技股份有限公司 | Comprehensive utilization method of microbial fermentation waste liquid |
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