CN104686595A - Preparation method and application of Poyang lake beach ashed sedge soaking liquid - Google Patents
Preparation method and application of Poyang lake beach ashed sedge soaking liquid Download PDFInfo
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- CN104686595A CN104686595A CN201510072976.4A CN201510072976A CN104686595A CN 104686595 A CN104686595 A CN 104686595A CN 201510072976 A CN201510072976 A CN 201510072976A CN 104686595 A CN104686595 A CN 104686595A
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- Prior art keywords
- sedge
- ashing
- soak
- beach
- poyang
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- 241001290610 Abildgaardia Species 0.000 title claims abstract description 49
- 238000002791 soaking Methods 0.000 title claims abstract description 9
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000007788 liquid Substances 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 230000005764 inhibitory process Effects 0.000 claims abstract description 5
- 238000004380 ashing Methods 0.000 claims description 43
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 241000192701 Microcystis Species 0.000 claims description 2
- 230000001629 suppression Effects 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 4
- 241000218093 Phedimus aizoon Species 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 3
- 238000012851 eutrophication Methods 0.000 description 3
- 239000008239 natural water Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000036531 allelopathy Effects 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 241000192710 Microcystis aeruginosa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000003619 algicide Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
A preparation method and application of Poyang lake beach ashed sedge soaking liquid are disclosed, wherein the method comprises the following steps: harvesting Poyang lake beach lichen ashmead, selecting the lichen ashmead, cutting the lichen ashmead to each section of 2-4 cm, soaking in distilled water at a soaking ratio of 6g of the lichen ashmead to 1L of distilled water for 14-18 days, and filtering to obtain a soaking solution. The invention has the advantages that: the Poyang lake beach ashed sedge soaking liquid has a strong algae inhibition rate, a short algae inhibition period and an obvious effect; the Poyang lake beach ashed sedge is abundant in resources and easy to obtain; the Poyang lake beach ashed sedum aizoon soaking liquid has the advantages of simple algae inhibition operation, simple maintenance and low cost.
Description
Technical field
The present invention relates to plant soaking solution ecological algae control field, be specifically related to a kind of preparation method and its usage of Poyang eyot beach ashing sedge soak.
Background technology
The blue-green alga bloom that eutrophication causes has become global environmental problem, and blue-green alga bloom causes aqueous bio chemical property to change and function of water body declines.The whole world on average about has the wawter bloom of 59% poisonous, in China's major part eutrophication water, blue-green alga bloom mainly to have superiority algae kind with microcystic aeruginosa, huge economic loss is caused to environment and social economy, effectively prevents blue-green alga bloom from being problem in the urgent need to address.The drawbacks such as current blue-green alga bloom is administered and mainly adopted physics, chemistry and biological method, as directly added chemical algicide (copper sulphate) to water body, easily produces secondary pollution, and processing cost is high, are difficult to the improvement of natural water body blue-green alga bloom.
Allelopathic algal control is a kind of plant secretion chemical substance entered environment, produces direct or indirect impact to another kind of plant.Current research finds that the culture fluid of aquatic lettuce (fragrant marshweed herb) and water sword-like leave produces inhibitory action to microcystic aeruginosa; Terrestrial plant such as the soaks such as straw, wheat straw, rice husk and Tibet peeling barley all have alga-inhibiting action to microcystic aeruginosa.The dislysate that K rner etc. study submerged plant favour flower watermifoil produces allelopathic alga-inhibiting action as medium to algae; Nakai etc. study spike watermifoil to the inhibitory action of microcystic aeruginosa, and under the effect that presses down of filtered fluid to microcystic aeruginosa obtaining spike watermifoil culture fluid is better than Dual culture, spike watermifoil is to the inhibitory action of microcystic aeruginosa.The defect of allelopathy algal control in operating cost, raw material acquisition and inhibiting rate etc., makes allelopathy algal control technology difficulty be applied to natural water body.
Statistics obtains Poyang eyot beach ashing sedge annual production more than 150,000 tons according to investigations, and exploitation Poyang eyot beach ashing sedge soak algal control technology has broad prospects, and improves the comprehensive utilization value of Poyang eyot beach ashing sedge.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and its usage of Poyang eyot beach ashing sedge soak, the soak prepared of the method can the growth of suppression microcystic aeruginosa of convenience and high-efficiency.Not only PROCESS FOR TREATMENT is simple, and aboundresources obtains convenient, and effect of algae restraint is obvious, and operating technology is simple, practical, the features such as energy saving economy.
For achieving the above object, the preparation method of Poyang eyot beach ashing sedge soak of the present invention, its method is: first gather in Poyang eyot beach ashing sedge, pick out ashing sedge, ashing sedge is cut off 2cm-4cm mono-section, then be dipped in distilled water, the weight of ashing sedge and distilled water is that 6g ashing sedge joins 1L distilled water, soak after 14 days-18 days, filter to obtain soak.
Described soak can be used for suppressing microcystic aeruginosa.
Soak suppresses the method for microcystic aeruginosa: soak and synthetic medium are mixed with the medium of different phytomass; get medium for subsequent use and add microcystic aeruginosa; in illumination box, temperature control 29 ± 0.5 DEG C, control light carry out aseptic culture; intensity of illumination 2500lx; Light To Dark Ratio 14h:10h; counted under microscope, and observation microcystis number is to analyze ashing sedge soak to the inhibition of microcystic aeruginosa.
In described medium, manual adjustment total nitrogen and total phosphorus concentration are respectively 2mg/L and 0.2mg/L.
0.45 μm of miillpore filter crossed by described soak.
Of the present inventionly to be a little:
1, Poyang eyot beach ashing sedge soak has stronger algal control rate, and the algal control cycle is short, Be very effective;
2, Poyang eyot beach ashing sedge aboundresources, obtains easy;
3, Poyang eyot beach ashing sedge soak obtains simple, and technical requirement is lower, workable, is beneficial to applying of this technology;
4, non-secondary pollution in blue-green alga bloom water body rendered to by Poyang eyot beach ashing sedge soak, easily degrades;
5, the processing of Poyang eyot beach ashing sedge soak is simple, saves human and material resources and financial resources;
6, Poyang eyot beach ashing sedge soak site requirements is lower;
7, the algal control of Poyang eyot beach ashing sedge soak runs simple, and safeguard simple, cost is low;
8, this ashing sedge soak algal control technology, is expected to realize control and the improvement that this technology is applied to natural water body blue-green alga bloom.
Embodiment
First gather in Poyang eyot beach ashing sedge and go back to laboratory, pick out ashing sedge, cut off about 2cm every section, put into 2500ml conical flask and add 2000mL distilled water continuous dipping about 16 days under normal temperature laboratory condition.
Take out soak and cross 0.45 μm of miillpore filter, then add BG11 medium by a certain percentage, obtain the medium of different phytomass, control total nitrogen and total phosphorus concentration in medium and be respectively 2mg/L and 0.2mg/L (with reference to eutrophication).
Cultivate at the illumination box of indoor temperature control, control light, control temperature 29 ± 0.5 DEG C, intensity of illumination 2500lx, Light To Dark Ratio 14h:10h, obtain Microcystis aeruginosa biomass and calculate ashing sedge soak to the inhibiting rate of microcystic aeruginosa, in table 1.Poyang eyot beach dominant plant withered ashing sedge and fresh ashing sedge soak as can be seen from Table 1, all Water extract effect is produced to microcystic aeruginosa, ashing sedge soaks phytomass when 6g/L, fresh ashing sedge and the inhibiting rate of withered ashing sedge soak to microcystic aeruginosa are greater than 80%, fresh ashing sedge soak algal control rate is all more than 90%, and more withered ashing sedge immersion algal control rate is higher.
Microcystic aeruginosa inhibiting rate in table 1 different ashing sedge biomass soak
| Soak biomass content (g/L) | 6 | 12 | 24 | 48 | 72 |
| Fresh sedge inhibiting rate (%) | 93.6 | 94.1 | 94.5 | 94.7 | 95.1 |
| Withered sedge inhibiting rate (%) | 81.3 | 83.6 | 85.3 | 87.0 | 87.4 |
Claims (5)
1. the preparation method of a Poyang eyot beach ashing sedge soak, it is characterized in that method is: first gather in Poyang eyot beach ashing sedge, pick out ashing sedge, ashing sedge is cut off 2cm-4cm every section, be dipped into again in distilled water, the quality of ashing sedge and distilled water is that 6g ashing sedge joins 1L distilled water with soaking volume ratio, soaks after 14 days-18 days, filters to obtain soak.
2. a purposes for Poyang eyot beach ashing sedge soak, is characterized in that: described soak can be used for suppressing microcystic aeruginosa.
3. the method for a Poyang eyot beach ashing sedge soak suppression microcystic aeruginosa, it is characterized in that: the medium that soak and synthetic medium are mixed with different phytomass is for subsequent use, add microcystic aeruginosa again in medium for subsequent use, in illumination box, temperature control 29 ± 0.5 DEG C, control light carry out aseptic culture, intensity of illumination 2500lx, Light To Dark Ratio 14h:10h, counted under microscope, observation microcystis number is to analyze ashing sedge soak to the inhibition of microcystic aeruginosa.
4. a kind of Poyang eyot beach ashing sedge soak according to claim 3 suppresses the method for microcystic aeruginosa, it is characterized in that: in described medium, total nitrogen and total phosphorus concentration are respectively 2mg/L and 0.2mg/L.
5. the preparation method of a kind of Poyang eyot beach ashing sedge soak according to claim 1, is characterized in that: 0.45 μm of miillpore filter crossed by described soak.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510072976.4A CN104686595A (en) | 2015-02-12 | 2015-02-12 | Preparation method and application of Poyang lake beach ashed sedge soaking liquid |
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| CN201510072976.4A CN104686595A (en) | 2015-02-12 | 2015-02-12 | Preparation method and application of Poyang lake beach ashed sedge soaking liquid |
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| CN104686595A true CN104686595A (en) | 2015-06-10 |
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| CN201510072976.4A Pending CN104686595A (en) | 2015-02-12 | 2015-02-12 | Preparation method and application of Poyang lake beach ashed sedge soaking liquid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557777A (en) * | 2015-12-17 | 2016-05-11 | 江西师范大学 | Poyang lake beach mushroom soaking liquid and method for inhibiting microcystis aeruginosa by using same |
| CN109845763A (en) * | 2019-02-14 | 2019-06-07 | 杭州师范大学 | A kind of algae-inhibiting agent preparation method and applications based on moss extracting solution |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969984A (en) * | 2006-12-07 | 2007-05-30 | 刘爱芹 | Externally applied medicament for treating acne and allergy |
| CN101513195A (en) * | 2008-02-21 | 2009-08-26 | 长江大学医学院 | Method for controlling propagation of schistosomiasis in lake regions |
| EP3003034A1 (en) * | 2013-05-30 | 2016-04-13 | Ohio State Innovation Foundation | Natural bioherbicides and related materials and methods |
-
2015
- 2015-02-12 CN CN201510072976.4A patent/CN104686595A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1969984A (en) * | 2006-12-07 | 2007-05-30 | 刘爱芹 | Externally applied medicament for treating acne and allergy |
| CN101513195A (en) * | 2008-02-21 | 2009-08-26 | 长江大学医学院 | Method for controlling propagation of schistosomiasis in lake regions |
| EP3003034A1 (en) * | 2013-05-30 | 2016-04-13 | Ohio State Innovation Foundation | Natural bioherbicides and related materials and methods |
Non-Patent Citations (2)
| Title |
|---|
| 张维昊: "菖蒲对铜绿微藻的化感作用", 《中国环境科学》 * |
| 贾晓会: "真核藻对铜绿微囊藻的化感抑制及其调控", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557777A (en) * | 2015-12-17 | 2016-05-11 | 江西师范大学 | Poyang lake beach mushroom soaking liquid and method for inhibiting microcystis aeruginosa by using same |
| CN105557777B (en) * | 2015-12-17 | 2018-04-03 | 江西师范大学 | Poyang lake beach mushroom soaking liquid and method for inhibiting microcystis aeruginosa by using same |
| CN109845763A (en) * | 2019-02-14 | 2019-06-07 | 杭州师范大学 | A kind of algae-inhibiting agent preparation method and applications based on moss extracting solution |
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Application publication date: 20150610 |
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