CN104666384A - Separating and purifying method for bioactive constituents of maca - Google Patents
Separating and purifying method for bioactive constituents of maca Download PDFInfo
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Abstract
The invention provides a method for separating and purifying bioactive constituents of maca root tubers. By optimizing maca raw materials and carrying out multi-stage wall dilapidation and extraction on the raw materials, the purposes of improving the yield of maca alkaloids in the raw materials and lowering the cost of the raw materials are realized. Moreover, the maca alkaloids obtained by the method are relatively high in proportion of components (such as maca amide) with a health care effect. Animal experiments verify that the product provided by the invention can be used for obviously improving the anti-fatigue capacity of animals, promoting the growth of the animals and regulating hormone secretion in a living body.
Description
Technical field
The present invention relates to the isolation and purification method of plant bioactive ingredients, the isolation and purification method of concrete a kind of Lepidinm meyenii Walp bioactive ingredients.
Background technology
Lepidinm meyenii Walp (Maca) has another name called Peruvian ginseng, maka etc., the annual or 2 years raw herbaceous plant in South America, belong to Cruciferae (Cruciferae) separate row Vegetable spp (Lepidium L), concrete kind is also disputable, generally believe in the world, Lepidium meyenii Walp and Lepidium peruvianum Chacon two kinds almost do not have difference on growth and breeding, phytochemistry and health-care effect, are therefore collectively referred to as " Maca " (transliteration is " Lepidinm meyenii Walp ").Lepidinm meyenii Walp has had the cultivation history of several thousand in mountain area, Andean, South America, is one of indispensable crop.There is good health-care effect.
Have resisting fatigue in Lepidinm meyenii Walp, strengthen the active component mainly Lepidinm meyenii Walp alkaloid of metabolism degree, it can improve the quantity of the quantity of animal mature follicle corpusculum, the mobility of sperm and sperm simultaneously, thus can significantly improve the fertility of mammal and Fish.And the alkaloid in Lepidinm meyenii Walp can act on hypothalamus and hypophysis cerebri, there is endocrine regulation gland as functions such as adrenal gland, thyroid, pancreas, ovaries, thus balance endocrine hormone, thus can with giving treatment female climacteric syndrome.Alkaloid kind in Lepidinm meyenii Walp plant is more, determined its molecular structure at present, can carry out qualitative and quantitative analysis be mainly a kind of alkaloid of macamide.
Common alkaloid extracting method has sour water direct extraction method, infusion process, oozes rumble method, hot cocurrent flow method, supersound extraction etc.At present, only have about the alkaloidal supersound extraction of Lepidinm meyenii Walp.Therefore, but adopt conventional plant supercritical ultrasonics technology process Lepidinm meyenii Walp, the alkaloidal productive rate of Lepidinm meyenii Walp is lower, causes that existing Lepidinm meyenii Walp alkaloid cost is high and health-care effect is not obvious.In addition, in the Lepidinm meyenii Walp biology total alkali that prior art extracts, composition is numerous and diverse, the activated kind of tool its containing quantity not sufficient, cause Maca extract aggregate performance to go out the more weak problem of health care.
Summary of the invention
In view of this, the present invention discloses a kind of alkaloid, the isolation and purification method that improves Lepidinm meyenii Walp alkaloid performance that effectively can extract Lepidinm meyenii Walp tuber.
Object of the present invention is achieved through the following technical solutions:
An isolation and purification method for Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 35 ~ 40 DEG C, relative humidity be 60 ~ 80% environment leave standstill 15 ~ 30 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 2 ~ 5mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3., in the container input of Lepidinm meyenii Walp tuber sealed, container is forced into 0.8 ~ 1.1MPa and is heated to 162 DEG C ~ 171 DEG C;
S4. in 0.5 ~ 1 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is-3 ~ 2 DEG C by the pure water of Lepidinm meyenii Walp tuber 1.3 ~ 3.4 times, quality is in the liquid coolant of Lepidinm meyenii Walp tuber 3 ~ 7 times;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 2 ~ 3 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 40 ~ 50 DEG C, under the condition of-100 ~-50KPa by 20% ~ 30% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
Lepidinm meyenii Walp tuber of the present invention preferably can originate from the Lepidinm meyenii Walp tuber (water content 70wt%) in mountain area, Andean, South America.In existing Lepidinm meyenii Walp tuber, Lepidinm meyenii Walp biology total alkali productive rate is low, and reason is mainly the poor by property of the higher and larger molecular organics of its cell wall strength of cell of Lepidinm meyenii Walp tuber.Therefore the present invention selects multiple step, carries out broken wall treatment to the cell of Lepidinm meyenii Walp tuber to the multistage.The present invention first the tuber of Lepidinm meyenii Walp is placed in high temperature, high pressure, anoxybiotic environment carry out pretreatment.Under above-mentioned condition, in Lepidinm meyenii Walp tuber, continuation metabolism is accumulated Lepidinm meyenii Walp alkaloid by irriate in a large number, and intracellular water content rises, cell wall rigidity increases, and finally makes cell wall be in an unsure state.Acidifying solution then reduces the heat stability of cell wall further, makes it easily more easily to break in subsequent treatment.To tuber heated under pressure, make ICP improve after release container in pressure, because ambient pressure declines suddenly, cell because of intrinsic pressure excessive and expand, cell wall spalling the most at last, complete first time breaking cellular wall.But for the cell of tuber fritter inside, the expansion due to cell is subject to the obstruction of peripheral cell, and cell wall can not spalling completely.Therefore the present invention designs employing liquid coolant especially, cools fast the cell of volumetric expansion.Cell and cell wall thereof volume contraction and broken broken into pieces at low temperatures, completes second time breaking cellular wall.In addition, K cryogenic treatment contributes to the mobility reducing cell membrane, makes it toughness and declines and easily dissipate in ultrasonic extraction and then make cellular content flow out cell.Fiber in broken cell debris, raw material all can hinder Lepidinm meyenii Walp alkaloid to be dissolved in solvent.Especially through the raw material of process of the present invention, its cell pulverization degree is high, and surface activity is strong and have stronger absorption property.The ultrasonic extraction of single is by the Lepidinm meyenii Walp alkaloid of large losses.Second order ultrasonic extraction then farthest can extract the bioactive ingredients in Lepidinm meyenii Walp raw material, improves productive rate and reduces production cost.Ultrasonic extraction in the present invention can select prior art and existing ultrasonic extraction equipment to realize.Under the condition of low temperature and low pressure, concentrated crude extract, can increase and have bioactive Lepidinm meyenii Walp alkaloid concentration in the product, strengthens the health properties of product.
Further, described first time ultrasonic extraction refer to liquid coolant is heated to 50 ~ 55 DEG C after adopt the ultrasonic extraction 30 ~ 50min of 90KHz;
After described second time ultrasonic extraction refers to and ethanol is heated to 45 ~ 55 DEG C, with the ultrasonic extraction 50 ~ 90min of 1000KHz.
Further, described acidifying solution comprises 60 ~ 80 parts of water, 1.2 ~ 3.5 parts of potassium dihydrogen phosphates, 0.2 ~ 1.1 part of ferrous succinate, 0.03 ~ 0.10 part of dodecylbenzene sodium sulfonate, 2 ~ 7 parts of sodium carboxymethyl cellulose by weight.
Potassium dihydrogen phosphate, ferrous succinate are prior art, for the formation of weak acid, nontoxic and stable acidifying solution system; Dodecylbenzene sodium sulfonate is conventional nontoxic, edible surfactant, contributes to the contact of acidifying solution and cell, cell wall.The present invention adds sodium carboxymethyl cellulose in acidifying solution, and it can infiltrate in raw material, be adsorbed on cell wall by the effect of dodecylbenzene sodium sulfonate.After being adsorbed by sodium carboxymethyl cellulose, the stability of cell wall declines further, and finally make the process of cell spalling more violent, cellular content is sprayed cell in large quantities.Sodium carboxymethyl cellulose is generally used for making food thickening agent, is prior art.After tested, adopt mass fraction be the acetic acid solution of 5% as acidifying solution, adopt mass fraction be the sodium chloride solution of 0.02% as liquid coolant, also have certain effect.
Preferably, described liquid coolant comprises 60 ~ 80 parts of water, 2 ~ 9 parts of potassium chloride, 0.001 ~ 0.005 part of potassium ferricyanide, 10 ~ 14 parts of ammonium bicarbonate by weight.
Potassium chloride and ammonium bicarbonate can reduce the freezing point of water, make water have low temperature and high fluidity concurrently simultaneously.Potassium ferricyanide and ammonium bicarbonate can also make main component be that pectin and cellulosic cell wall harmomegathus strengthen jointly, and violent contraction and broken occurs final cell wall at low temperatures.Described potassium ferricyanide is a kind of inorganic compound, and chemical formula is K
3[Fe (CN)
6], be commonly called as potassium ferricyanide, red potassium prussiate, Hexacyanoferrate potassium.Nontoxic at low temperatures.Ammonium bicarbonate can neutralize residual acidifying solution, makes solution entirety present alkalescence.
Further, the preparation method of described acidifying solution, for after heating water to 90 DEG C, adds described sodium carboxymethyl cellulose, potassium dihydrogen phosphate and dodecylbenzene sodium sulfonate, keeps 10 ~ 20Min, be cooled to 50 DEG C, add described ferrous succinate and obtain described acidifying solution; Described Lepidinm meyenii Walp tuber is immersed in acidifying solution refer to by Lepidinm meyenii Walp tuber soak be in the acidifying solution of 40 ~ 50 DEG C, be placed in glass container, throwing in 40 ~ 60 particle diameters in often liter of acidifying solution is the bead of 2 ~ 8mm, with the speed oscillation 3 ~ 9min of 300 turns/min in rotary shaker.
The invention provides the method for bioactive ingredients in a kind of separation and purification Lepidinm meyenii Walp tuber, by preferred Lepidinm meyenii Walp raw material, carry out multistage breaking cellular wall and extraction to raw material, final realization improves Lepidinm meyenii Walp alkaloid productive rate in raw material, reduces cost of material object.In the Lepidinm meyenii Walp alkaloid that method of the present invention obtains simultaneously, composition (as macamide) ratio with health role is higher, show through zoopery, product provided by the invention can significantly improve animal anti-fatigue ability, promote that it grows, and the secretion of organism internal hormone can be regulated.
Detailed description of the invention
The present invention to be described in further detail below in conjunction with embodiment for the ease of it will be appreciated by those skilled in the art that:
Embodiment 1
The present embodiment provides a kind of isolation and purification method of Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 38 DEG C, relative humidity be 69% environment leave standstill 20 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 2 ~ 5mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3. Lepidinm meyenii Walp tuber is dropped in the container of sealing, container is forced into 0.8MPa and is heated to 165 DEG C;
S4. in 0.5 ~ 1 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is 2 DEG C by the pure water of Lepidinm meyenii Walp tuber 3 times, quality is in the liquid coolant of Lepidinm meyenii Walp tuber 4 times;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 3 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 45 DEG C, under the condition of-70KPa by 25% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
Described first time, ultrasonic extraction adopted the ultrasonic extraction 40min of 90KHz after referring to and liquid coolant being heated to 53 DEG C;
After described second time ultrasonic extraction refers to and ethanol is heated to 50 DEG C, with the ultrasonic extraction 80min of 1000KHz.
Described acidifying solution comprises 75 parts of water, 2 parts of potassium dihydrogen phosphates, 1 part of ferrous succinate, 0.06 part of dodecylbenzene sodium sulfonate, 5 parts of sodium carboxymethyl cellulose by weight.
Described liquid coolant comprises 77 parts of water, 4 parts of potassium chloride, 0.005 part of potassium ferricyanide, 12 parts of ammonium bicarbonate by weight.
The preparation method of described acidifying solution, for after heating water to 90 DEG C, adds described sodium carboxymethyl cellulose, potassium dihydrogen phosphate and dodecylbenzene sodium sulfonate, keeps 17Min, be cooled to 50 DEG C, add described ferrous succinate and obtain described acidifying solution; Described Lepidinm meyenii Walp tuber is immersed in acidifying solution refer to by Lepidinm meyenii Walp tuber soak be in the acidifying solution of 45 DEG C, be placed in glass container, throwing in 55 grain (directly) footpaths in often liter of acidifying solution is the bead of 5mm, with the speed oscillation 8min of 300 turns/min in rotary shaker.
Embodiment 2
The present embodiment provides a kind of isolation and purification method of Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 40 DEG C, relative humidity be 80% environment leave standstill 30 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 2mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3. Lepidinm meyenii Walp tuber is dropped in the container of sealing, container is forced into 1.1MPa and is heated to 162 DEG C ~ 171 DEG C;
S4. in 0.5 ~ 1 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is 2 DEG C by the pure water of Lepidinm meyenii Walp tuber 1.3 times, quality is in Lepidinm meyenii Walp tuber 3 times of liquid coolants;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 2 ~ 3 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 50 DEG C, under the condition of-100KPa by 30% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
In the present embodiment, ultrasonic extraction is the plant ultrasonic extraction of prior art, as the method disclosed in the extracting method of Chinese patent total alkaloids in Chinese caterpillar fungus and purposes (application number 200910032092.0).
Described acidifying solution comprises 60 parts of water, 3.5 parts of potassium dihydrogen phosphates, 0.2 part of ferrous succinate, 0.10 part of dodecylbenzene sodium sulfonate, 2 parts of sodium carboxymethyl cellulose by weight.
The preparation method of described acidifying solution, for after heating water to 90 DEG C, adds described sodium carboxymethyl cellulose, potassium dihydrogen phosphate and dodecylbenzene sodium sulfonate, keeps 20Min, be cooled to 50 DEG C, add described ferrous succinate and obtain described acidifying solution; Described Lepidinm meyenii Walp tuber is immersed in acidifying solution refer to by Lepidinm meyenii Walp tuber soak be, in the acidifying solution of 40 DEG C, to be placed in glass container, in often liter of acidifying solution throw in 60 particle diameters be the bead of 2mm, with the speed oscillation 9min of 300 turns/min in rotary shaker.
Embodiment 3
The present embodiment provides a kind of isolation and purification method of Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 35 DEG C, relative humidity be 80% environment leave standstill 15 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 5mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3. Lepidinm meyenii Walp tuber is dropped in the container of sealing, container is forced into 0.8MPa and is heated to 162 DEG C;
S4. in 0.5 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is-3 DEG C by the pure water of Lepidinm meyenii Walp tuber 3.4 times, quality is in Lepidinm meyenii Walp tuber 7 times of liquid coolants;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 2 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 50 DEG C, under the condition of-100KPa by 20% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
In the present embodiment, ultrasonic extraction is the plant ultrasonic extraction of prior art, as a kind of in Chinese patent with the method disclosed in the alkaloidal method of microwave ultrasonic wave compound collecting Lepidinm meyenii Walp (application number 201410014832.9).
The acidifying solution of the present embodiment is prior art.Carbon acid solution can be preferably.
Described liquid coolant comprises 80 parts of water, 2 parts of potassium chloride, 0.005 part of potassium ferricyanide, 10 parts of ammonium bicarbonate by weight.
Comparative example 1
The method that this comparative example adopts South China Science & Engineering University Du GUANGXIANG " the alkaloidal technical study of ultrasonic extraction Lepidinm meyenii Walp " (guangdong agricultural science, the 3rd phase in 2011) to provide extracts Lepidinm meyenii Walp biology total alkali.
Comparative example 2
This comparative example provides a kind of isolation and purification method of Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 40 DEG C, relative humidity be 60 ~ 80% environment leave standstill 25 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 3mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3., in the container input of Lepidinm meyenii Walp tuber sealed, container is forced into 0.9MPa and is heated to 166 DEG C;
S4. in 0.8 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is-1 DEG C by the pure water of Lepidinm meyenii Walp tuber 2.5 times, quality is in Lepidinm meyenii Walp tuber 5 times of liquid coolants;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 3 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 45 DEG C, under the condition of-50KPa by 20% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
In this comparative example, ultrasonic extraction is the plant ultrasonic extraction of prior art, as the method disclosed in the extracting method of Chinese patent total alkaloids in Chinese caterpillar fungus and purposes (application number 200910032092.0).
Described acidifying solution comprises 75 parts of water, 3 parts of potassium dihydrogen phosphates, 0.5 part of ferrous succinate, 0.05 part of dodecylbenzene sodium sulfonate by weight.
Comparative example 3
This comparative example provides a kind of isolation and purification method of Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 40 DEG C, relative humidity be 80% environment leave standstill 15 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 5mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3., in the container input of Lepidinm meyenii Walp tuber sealed, container is forced into 0.8MPa and is heated to 171 DEG C;
S4. in 0.5 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is-3 DEG C by the pure water of Lepidinm meyenii Walp tuber 3.4 times, quality is in Lepidinm meyenii Walp tuber 7 times of liquid coolants;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 2 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 50 DEG C, under the condition of-100KPa by 30% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
In this comparative example, ultrasonic extraction is the plant ultrasonic extraction of prior art.
The acidifying solution of the present embodiment is prior art.Carbon acid solution can be preferably.
Described liquid coolant comprises 60 parts of water, 9 parts of potassium chloride, 0.001 part of potassium ferricyanide, 14 parts of ammonium bicarbonate by weight.
The biology total alkali extract Lepidinm meyenii Walp raw material in embodiment 1,2,3 and comparative example 1,2,3 and macamide content measure, and its result is as shown in table 1.
Table 1 biology total alkali and macamide output
Adult male mice (body weight 113.11 ± 5.72g) is divided into 7 groups, often organizes 50, often group gavage feeding embodiment 1, embodiment 2, embodiment 3, comparative example 1, comparative example 2, comparative example 3 and normal saline respectively, feeding dosage is 0.5g.Feeding process lasts 50 days.
Often group chooses 30 mices, orders about its swimming, test mouse blood sugar concentration after 120min in being gone in off the deep end.Its result is as shown in table 2.
The biological activity of table 2 Maca extract
Be more than wherein specific implementation of the present invention, it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these apparent replacement forms all belong to protection scope of the present invention.
Claims (5)
1. an isolation and purification method for Lepidinm meyenii Walp bioactive ingredients, comprises the following steps:
S1. Lepidinm meyenii Walp tuber is placed in anoxybiotic, 35 ~ 40 DEG C, relative humidity be 60 ~ 80% environment leave standstill 15 ~ 30 days;
S2. Lepidinm meyenii Walp tuber is pulverized as particle diameter is the fritter of 2 ~ 5mm, be immersed in the acidifying solution that quality is equal to Lepidinm meyenii Walp tuber;
S3. Lepidinm meyenii Walp tuber is dropped in the container of sealing, container is forced into 0.8 ~ 1.1MPa and is heated to 162 DEG C ~ 171 DEG C;
S4. in 0.5 ~ 1 second, the pressure of container is reduced to 0Pa, employing quality is that the material elutes in container to temperature is-3 ~ 2 DEG C by the pure water of Lepidinm meyenii Walp tuber 1.3 ~ 3.4 times, quality is in the liquid coolant of Lepidinm meyenii Walp tuber 3 ~ 7 times;
S5. adopt ultrasonic extraction to carry out first time ultrasonic extraction to the liquid coolant obtained in S4, filter acquisition first filtrate; Adding quality is that the ethanol of filtering residue 2 ~ 3 times carries out second time ultrasonic extraction to filtering residue, filters acquisition second filtrate; Merge the first filtrate and the second filtrate, obtain crude extract;
S6. 40 ~ 50 DEG C, under the condition of-100 ~-50KPa by 20% ~ 30% of its original volume of crude extract simmer down to, obtain Lepidinm meyenii Walp bioactive ingredients finished product.
2. method according to claim 1, is characterized in that: described first time ultrasonic extraction adopt the ultrasonic extraction 30 ~ 50min of 90KHz after referring to and liquid coolant being heated to 50 ~ 55 DEG C;
After described second time ultrasonic extraction refers to and ethanol is heated to 45 ~ 55 DEG C, with the ultrasonic extraction 50 ~ 90min of 1000KHz.
3. method according to claim 1 and 2, is characterized in that: described acidifying solution comprises 60 ~ 80 parts of water, 1.2 ~ 3.5 parts of potassium dihydrogen phosphates, 0.2 ~ 1.1 part of ferrous succinate, 0.03 ~ 0.10 part of dodecylbenzene sodium sulfonate, 2 ~ 7 parts of sodium carboxymethyl cellulose by weight.
4. method according to claim 1 and 2, is characterized in that: described liquid coolant comprises 60 ~ 80 parts of water, 2 ~ 9 parts of potassium chloride, 0.001 ~ 0.005 part of potassium ferricyanide, 10 ~ 14 parts of ammonium bicarbonate by weight.
5. method according to claim 3, it is characterized in that: the preparation method of described acidifying solution is for after heating water to 90 DEG C, add described sodium carboxymethyl cellulose, potassium dihydrogen phosphate and dodecylbenzene sodium sulfonate, keep 10 ~ 20Min, be cooled to 50 DEG C, add described ferrous succinate and obtain described acidifying solution; Described Lepidinm meyenii Walp tuber is immersed in acidifying solution refer to by Lepidinm meyenii Walp tuber soak be in the acidifying solution of 40 ~ 50 DEG C, be placed in glass container, throwing in 40 ~ 60 particle diameters in often liter of acidifying solution is the bead of 2 ~ 8mm, with the speed oscillation 3 ~ 9min of 300 turns/min in rotary shaker.
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