CN103773596B - The preparation method of krill oil - Google Patents
The preparation method of krill oil Download PDFInfo
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- CN103773596B CN103773596B CN201310754432.7A CN201310754432A CN103773596B CN 103773596 B CN103773596 B CN 103773596B CN 201310754432 A CN201310754432 A CN 201310754432A CN 103773596 B CN103773596 B CN 103773596B
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Abstract
The preparation method who the invention discloses a kind of krill oil, comprises the following steps: (1) mixes krill with extractant, shears; The addition of extractant is the extractant that adds 1L in the krill of every 0.5-1.5kg; (2) Separation of Solid and Liquid, obtains shrimp mud; (3) shrimp mud is mixed with extractant, extract, obtain extract, desolvation; The addition of extractant is the extractant that adds 1-2L in the krill of every 1kg; Extractant is C2-4Alcohol or C2-4The aqueous solution of alcohol. This preparation method does not need the dehydration through existing routine, and preparation method is simple, and the krill good fluidity, the transparency that make are high, and water-solubility impurity is few, and content of phospholipid is more than 40%, and in phosphatide, lecithin content is more than 60%.
Description
Technical field
The present invention relates to a kind of preparation method of krill oil.
Background technology
Krill is the krill of one way of life in waters, the Antarctic Continent, is also that on the earth, quantity maximum is the most ripeSingle one of living resources of planting. The krill oil extracting from krill is rich in phosphatide (mainly with lecithinBe main) etc. composition, and the aliphatic acid of phosphatide mainly comprises eicosapentaenoic acid (EPA) and 22 carbonAcid (DHA), the content sum of the two accounts for the more than 40% of fatty acids in phospholipids total amount. South Pole phosphorusShrimp sauce has the angiocardiopathy of reduction, prevention senile dementia and improves the multiple physiologically actives such as immunity of organisms,And its activity is better than triglyceride type EPA/DHA product in the market and terrestrial plant sourceLecithin product (not containing EPA and DHA in its aliphatic acid composition).
Unrighted acid DHA (DHA) is mainly combined in glyceryl 2 of phosphatide(Sn2 position) is upper, and DHA is the basis that forms brain cell membrane, the division to brain cell, propagation, nerveThe g and D of conduction, cynapse plays a part very important, is that human brain forms and IQ exploitationEssential material. And DHA is to vision, brain activity, fat metabolism, fetal growth and exempt fromEpidemic disease function and avoid senile dementia to have very big impact. DHA when shortage, can cause series of symptoms,Comprise growth retardation, the skin abnormality scales of skin that peel off, sterile, dysnoesia etc.
But common DHA arranges in random mode, in unordered state, cannot guaranteeEntirely be absorbed by the body, the DHA on Sn1, Sn3 position is digested in human body conventionally, produces heat, onlyThere is the DHA on Sn2 position to be absorbed completely by human body. In krill oil, DHA is combined in phosphatideOn glyceryl Sn2 position, therefore can be absorbed the life of the DHA on the Sn2 position of krill completely by peopleThing is active and bioavilability is very high, is 3-5 times of common fish oil DHA activity.
By krill oil be simultaneously raw material by the way of biological enzyme, can change into phosphatidylserine (PS).The function of phosphatidylserine (PS) is mainly to improve nerve cell function, regulates the conduction of nerve impulse,Promote brain memory function. Relevant expert generally acknowledges: from unrighted acid 22 carbon in krill oil sourceIt is upper that six alkene (DHA) are attached to phosphatidylserine (PS), and the two comprehensive function, can improve brainNerve cell function and brain activity, improve memory and promote infant's brain to grow and safeguard that brain is strongHealth, has great economy and theory value.
Based on the critical role of krill resource, countries in the world are all using krill as important foodResource, and start expedition krill in the phase at the beginning of the sixties in last century. Produce so far, krill is totalCatch has exceeded 6,000,000 tons. But at present,, the krill that fish for countries in the world is processed to mostlyThe feed of aquaculture, its economic worth and social value find full expression far away. In energy prices andUnder the realistic background that labor cost etc. sharp rise, make full use of the krill oil resource in krill,Exploitation high value added product is the important channel that krill utilizes.
Enter after 21st century the krill oil demand rapid growth of market to medical-grade, krill industryCatch this opportunity. Owing to containing abundant phosphatide in krill oil, nutrient and healthcare products industries are attractedShow great attention to. The fast development of krill health-product market and other marine organism health care product have formed day simultaneouslyThe market competition that benefit is fierce. The AkerBiomarine company of Norway and Canadian NeptuneTechnologies company is global main at present medical-grade krill oil manufacturing enterprise. Particularly AkerBiomarine company, the enterprise that marine product of Qi Shi Norway is fished for and processed, is engaged in high-end krill and producesThe research and development of product, production and selling are existing for many years, and only 2009,100 tons of left and right were just sold by companyKrill oil health products.
Domesticly be engaged in the recent period krill oil research and development and production unit gets more and more. The extraction side of krill oil in the pastMethod is that hot-pressing method fork-like farm tool used in ancient China extracts, but this method yield is low, and operating temperature is high, and product quality is had to impact,Be eliminated gradually. In prior art, generally adopt taking dry krill meal (moisture 8%-10%) as formerMaterial, extract krill oil with solvent method, but the method also has certain defect, the first, krill meal pairHave strict requirement in water content, General Requirements water content is at 8%-10%, because water content is too high to carryingThe rate of getting can have a great impact, and can make a large amount of harmful water solubility impurities can enter in extract,Increase post processing cleaning procedure difficulty; The second, krill body contains a kind of enzyme, after fishing for, normalIn rule lower a few hours of temperature, can, rapidly protein digestion in krill, become noxious material, be commonly called as krillSelf-dissolving, the krill after self-dissolving can not human consumption, therefore the preparation of krill meal is generally on dredgerCarry out, but dredger space is little, treating capacity is limited, adds market large for the demand of krill meal,There is the phenomenon that supply falls short of demand in krill meal, expensive.
On the other hand, on dredger, can also krill is freezing to below-20 DEG C, to freeze shrimp rapidlyForm storage and transport. In prior art, it is that raw material directly extracts krill oil that people also attempt freezing shrimp.Extracting method is generally first to remove moisture, then extracts krill oil. The method of removing moisture generally adopts low temperatureThe dehydration of desivac fork-like farm tool used in ancient China, the method operating temperature low (30 degree are following), dewatering time is long, and cost is higher.Another kind of dewatering is thermal dehydration under vacuum, and the method power consumption is large, and due to phosphatide in krill oilBe heat-sensitive substance, temperature is high can decompose in a large number, makes the dehydration temperaturre can not be too high, thereby while making to dewaterBetween become very long, generally need 7-8 hour, be not suitable for large-scale industrial production.
And, obtain after krill oil in extraction, the process such as also need to refine, remove in krill oilImpurity. In a word, the extracting method extraction process step complexity of krill oil in prior art, composition is very high.This phenomenon has increased the making composition of phosphatide, urgently to be resolved hurrily. Therefore, develop-kind directly with freezing southUtmost point krill is raw material, and preparation technology is simple, and the method that time consumption and energy consumption is few, extracts krill oil, isVery urgent.
Summary of the invention
Technical problem to be solved by this invention is to overcome in prior art and extracts taking krill meal as raw materialThe method of krill oil is large to the demand of krill meal, and raw material supply is inadequate, is raw material and adopt freezing krillExtract krill oil preparation time long, the defect that energy consumption is large, provides a kind of preparation method of krill oil, described inPreparation method do not need the dehydration through existing routine, preparation method is simple, the krill makingOil mobility is good, transparency is high, and water-solubility impurity (as the material such as polysaccharide, low molecular polypeptide) is few, carriesThe rate of getting obviously improves, and content of phospholipid is more than 40%, and in phosphatide, lecithin content is more than 60%.
In prior art, in the extracting method of krill oil, the dewatering dewatering time of krill is long, and costHigh. On this basis, inventor removes moisture by the extractant that adds certain concentration in krill.But great many of experiments shows: extractant removes for moisture in krill, and not as easy in the imagination.In great many of experiments, the recovery rate of krill oil is almost 0, that is to say: simple extractant, basic nothingMethod is extracted and is obtained krill oil. Its former because: ethanol can extract moisture and phosphatide simultaneously, but the present inventionBut need moisture and phosphatide to separate, obtain target extract---phosphatide, and, when first moisture can not beWhile being removed, cannot extracting and obtain phosphatide. This is also in prior art, still not use C2-4AlcoholRemove the reason of moisture in krill.
But inventor is through further research, finds: the extractant of specific consumption, then coordinate specialFixed processing step and condition, not only can remove the moisture in krill, and can not cause the stream of phosphatideLose, on this basis, can also remove the impurity in krill oil, thus, do not need through refiningProcess just can obtain the krill oil that purity is high, and the recovery rate of krill oil is also improved, and has realized at one stroke manyThe effect obtaining. So far, just obtained technical scheme of the present invention.
The object of the invention is to: a kind of preparation method of krill oil is provided, and described preparation method comprisesFollowing steps:
(1) krill is mixed with extractant, obtain mixture A, shear; Described extractant isC2-4Alcohol or C2-4The aqueous solution of alcohol, the phosphorus that the addition of described extractant is every 0.5-1.5kgIn shrimp, add the extractant of 1L;
(2) Separation of Solid and Liquid, obtains shrimp mud;
(3) described shrimp mud is mixed with extractant, obtains mixture B, extract, obtain extract,Desolvation; Described extractant is C2-4Alcohol or C2-4The aqueous solution of alcohol, describedThe addition of extractant is the extractant that adds 1-2L in the krill of every 1kg.
Be described further for above-mentioned preparation method below:
In step (1), before described krill is mixed with extract, preferably to described phosphorusShrimp carries out certain pretreatment; Described pretreated method is the method for this area routine, as cleaned,Drain etc. The described method draining is the method for this area routine, preferably for naturally draining.
Those skilled in the art all understand: because krill self-dissolving is at normal temperatures rapid, quality changes very fast,Generally speaking need freezing storage; In the time that described krill is freezing krill, preferably need krill to enterRow thaws, then drains. The described method of thawing is the method for this area routine, is preferably at 10-20 DEG CUnder condition, described freezing krill is mixed with water, soaks. The described time of thawingFor the time of this area routine, be preferably in 30min; The consumption of described water is this area routineConsumption, be preferably the freezing krill described in not have. The method that immersion is thawed compares krill at skyIn gas, naturally thaw or heat thaw effective. After described thawing, preferably to describedAfter krill is cleaned, then drain. Generally speaking, described thaws to the process draining, and can remove krillA large amount of water-solubility impurity on surface, as NaCl, thereby reduces the impurity content in finished product.
In the present invention, described alcohol is preferably C2-3Alcohol, be more preferably ethanol, normal propyl alcohol and isopropylOne or more in alcohol.
In step (1), the addition of described extractant preferably adds 1L in the krill for every 1kgExtractant.
In step (1), after described mixing, the content of the alcohol in described mixture A is betterGround is 40%-80%, is more preferably 50%-70%; Described percentage is that described alcohol accounts for described mixingThe percent by volume of the liquid in thing A.
In step (1), after mixing, krill fully contacts with extractant, large water gaging in krillDivide and discharge, Extractant concentration further reduces, and under this concentration, krill oil can not be leached, and in shrimpImpurity (as polysaccharide, low molecular polypeptide etc.) can be leached. This step has not only removed in krillMoisture for the extraction of follow-up krill is laid a good foundation, can be removed the impurity in krill simultaneously, extracts to obtain phosphorusAfter shrimp sauce without carrying out again loaded down with trivial details subtractive process consuming time.
In step (1), the concentration of the aqueous solution of described alcohol is preferably more than 90%, to be more preferably95%, described percentage is the percent by volume that described alcohol accounts for the aqueous solution of alcohol.
In step (1), preferably, before described shearing, by described krill and described extractantStir, and tentatively pulverize. The method of described pulverizing is preferably for being used gear pump to pulverize.
In step (1), preferably, the method for described shearing and condition are the method for this area routineAnd condition. The speed of described shearing is preferably 5000-12000rpm, is more preferably 10000rmp.The time of described shearing is preferably 5-15min, is more preferably 10min. Described shearing preferablyIn high-speed shearing machine, carry out.
In step (2), the method for described Separation of Solid and Liquid and condition are method and the bar of this area routinePart. The method of described Separation of Solid and Liquid is preferably centrifugation; The separation factor of described centrifuge (fromThe ratio of mental and physical efforts and gravity) preferably for being greater than 2500G, be more preferably 2500-3000G. Described is centrifugalThe time of staying separating is preferably 10-20min, is more preferably 15min. Described centrifugation is betterGround is for using desk centrifuge or horizontal screw centrifuge centrifugal.
In step (2), preferably, what described Separation of Solid and Liquid obtained can be according to containing the liquid of alcoholThe recovery method of the alcohols material of this area routine reclaims; Described recovery method is preferably distillation;Described distillation preferably for to adopt tower equipment to carry out under normal pressure.
In step (3), the addition of described extractant preferably adds in the krill for every 1kgAdd the extractant of 1L.
In step (3), after described mixing, the content of the alcohol in described mixture B is betterGround is 90%-95%, and described percentage is the volume that described alcohol accounts for the liquid in described mixture BPercentage.
In step (3), described extraction is preferably extracted for stirring. The time of described extraction is betterGround is 20-40min, is more preferably 30min.
In step (3), preferably, the number of times of described extraction is the number of times of this area routine, described inThe number of times of extraction be preferably 2-3 time. In the time that described extraction time is greater than 1 time, according to this areaRoutine, merges extract.
In step (3), the method for described desolvation and condition are method and the bar of this area routinePart; The method of described desolvation is preferably vacuum removal solvent under the temperature conditions below 60 DEG C.The described temperature of deviating from solvent is preferably 40-55 DEG C. The time of described desolvation is preferably10-30min. Described desolvation is preferably taken out and rubbing board oil-sealed rotary pump at water-ring vacuum pump successivelyIn carry out, or carry out in revolution rubbing board evaporimeter.
Meeting on the basis of this area general knowledge, above-mentioned each optimum condition, can be combined, i.e. get Ben FaBright each preferred embodiments.
Agents useful for same of the present invention and raw material be commercially available obtaining all.
Positive progressive effect of the present invention is: the extracting method preparation method of krill oil of the present invention is simple,The krill oil good fluidity, the transparency that make are high, and water-solubility impurity is (as the thing such as polysaccharide, low molecular polypeptideMatter) few, recovery rate obviously improves, and content of phospholipid is more than 40%, and in phosphatide, lecithin content is more than 60%.
Detailed description of the invention
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention toAmong described scope of embodiments. The experimental technique of unreceipted actual conditions in the following example, according to oftenRule method and condition, or select according to catalogue.
Embodiment 1
(1) hydrostatic thaws: get the freezing krill of 190g, be added to containing in water-soluble device, 18 DEG C of water temperaturesUnder condition, hydrostatic soaks after 15min, freezes shrimp and melts, and now water temperature is reduced to 12 DEG C of left and right; Use funnel dropSolid carbon dioxide part, uses a small amount of 4～6 DEG C of pure water drip washing if desired again, except the NaCl of deentrainment;
(2) 95% ethanol of 200mL and the shrimp body draining are mixed, obtain mixed liquor A, mixed liquor AThe content of middle ethanol is the percent by volume that 60%(ethanol accounts for the liquid in mixture A), pulverize, addEnter to high shear machine and shear 10min at 10000rpm;
(3) with desk centrifuge centrifugal 15min under 3000G centrifugal force, obtain shrimp mud;
(4) to 95% ethanol that adds 200mL in shrimp mud, mix, obtain mixture B, mixIn thing B, the content of ethanol is the percent by volume that 90%(ethanol accounts for the liquid in mixture B), stir30min, static layering, elimination ethanol phase; Add again the absolute ethyl alcohol of 200mL, repeat to extract, mergeSecondary raffinate; At 60 DEG C, in glass revolution evaporimeter, vacuumize with water-ring vacuum pump, de-Remove ethanol, then use rubbing board oil-sealed rotary pump, under high vacuum condition, continue to remove residual ethanol and micro-Water gaging divides (10 points of kinds).
The product making is reddish brown, can flow, and this product energy encapsulating ball, capsule and pill inclusion is not aobvious mixedTurbid, illustrate that the product making is transparence, Product Status is good.
Recording content of phospholipid in krill oil product with molybdenum blue colorimetric method is 41%, lecithin content in phosphatide70%。
Embodiment 2
(1) hydrostatic thaws: get the freezing krill of 400g, be added to containing in water-soluble device, 20 DEG C of water temperaturesUnder condition, hydrostatic soaks 15min, freezes shrimp and melts; Use funnel drain well, use again if desired a small amount of 4～6 DEG CPure water drip washing;
(2) isopropyl alcohol of 500mL is joined to above-mentioned thawing in the shrimp body draining, fully mix,Mixed liquor A, in mixed liquor A, the content of isopropyl alcohol is that 70%(isopropyl alcohol accounts for the liquid in mixture APercent by volume), pulverize, and join high shear machine at 10000rpm down cut 10min;
(3) with desk centrifuge, centrifugal 15min under 3000G centrifugal force, obtains shrimp mud;
(4) to the isopropyl alcohol that adds 500mL in shrimp mud, mix, obtain mixture B, mixtureIn B, the content of isopropyl alcohol is the percent by volume that 90%(isopropyl alcohol accounts for the liquid in mixture B), stirMix 30min, static layering, elimination isopropyl alcohol phase; Add again the isopropyl alcohol of 500mL, repeat to extract, closeAnd secondary raffinate, at 60 DEG C, in glass revolution evaporimeter, vacuumize de-with water-ring vacuum pumpIsopropyl alcohol, then vacuumize with rubbing board oil-sealed rotary pump, under high vacuum condition, continues to remove residual differentPropyl alcohol and de-micro-moisture (15min).
The product making is reddish brown, can flow, and this product energy encapsulating ball, capsule and pill inclusion is not aobvious mixedTurbid, illustrate that the product making is transparence, Product Status is good.
Recording content of phospholipid in krill oil product with molybdenum blue colorimetric method is 43%, lecithin content in phosphatide65%。
Embodiment 3
(1) hydrostatic thaws: get 500 kilograms of freezing krills, join 1500 liters of stainless steels and extractIn narrow-necked earthen jar, after topped up with water, the static 20min that thaws under 15 DEG C of conditions of water temperature, freezes shrimp and melts; From narrow-necked earthen jar bottomEmit immersion water, then with the pure water drip washing of 100 liters 4～6 DEG C and drain;
(2) 500 liter of 95% ethanol is joined to above-mentioned stainless steel and extract in narrow-necked earthen jar, mix, obtain mixedClose liquid A, in mixed liquor A, the content of ethanol is the volume that 70%(ethanol accounts for the liquid in mixture APercentage), play circulation with gear pump, make two-phase fully be uniformly mixed pulverizing, then insert high from topCutter, 15min turns round under 10000rpm;
(3) start horizontal screw centrifuge, the material in gear pump joined in horizontal screw centrifuge entrance,Flow out respectively ethanol and the very low shrimp mud of water capacity at two ends of centrifuge;
(4) shrimp mud is joined together with 500 liters of absolute ethyl alcohols to stainless steel and extracts in narrow-necked earthen jar, mix,Mixture B, in mixture B, the content of ethanol is that 90%(ethanol accounts for liquid in mixture BPercent by volume) stir 30min leach, static layering, elimination alcohol phase; Add again 500 liters anhydrousEthanol, repeats to extract, and merges extract, removes ethanol with turning round rubbing board evaporimeter under vacuum condition.In process, with the heating of low-pressure steam chuck, collect the oily flowing out from revolution rubbing board base of evaporator exitLiquid krill; ; After filter residue in stainless steel narrow-necked earthen jar is taken out, with the de-ethanol of 80 DEG C of hot-airs, de-Dry after being dissolved into can be used as feed and uses.
The product making is reddish brown, can flow, and this product energy encapsulating ball, capsule and pill inclusion is not aobvious mixedTurbid, illustrate that the product making is transparence, Product Status is good.
The content of phospholipid recording with molybdenum blue colorimetric method is 40.5%, and in phosphatide, lecithin content is more than 70%.
Claims (17)
1. a preparation method for krill oil, is characterized in that: described preparation method comprises following stepRapid:
(1) krill is cleaned, drained, mix with extractant, obtain mixture A, shear; InstituteThe extractant of stating is C2-4Alcohol or C2-4The aqueous solution of alcohol, the addition of described extractant is everyIn the krill of 0.5-1.5kg, add the extractant of 1L; In the time that described krill is freezing krill, to krillThaw, then clean, drain; The described method of thawing is: under 10-20 DEG C of condition, by instituteThe freezing krill of stating mixes with water, soaks; After described mixing, described mixingThe content of the alcohol in thing A is 40%-80%; Described percentage is that described alcohol accounts for described mixture AIn the percent by volume of liquid; The speed of described shearing is 5000-12000rpm;
(2) Separation of Solid and Liquid, obtains shrimp mud; The method of described Separation of Solid and Liquid is centrifugation; DescribedThe separation factor of centrifugation is for being greater than 2500;
(3) described shrimp mud is mixed with extractant, obtains mixture B, extract, obtain extract,Desolvation; Described extractant is C2-4Alcohol or C2-4The aqueous solution of alcohol, describedThe addition of extractant is the extractant that adds 1-2L in the krill of every 1kg; After described mixing,The content of the alcohol in described mixture B is 90%-95%, and described percentage is described in described alcohol accounts forMixture B in the percent by volume of liquid.
2. preparation method as claimed in claim 1, is characterized in that: described alcohol is C2-3Alcohol.
3. preparation method as claimed in claim 2, is characterized in that: described alcohol is ethanol, justOne or more in propyl alcohol and isopropyl alcohol.
4. preparation method as claimed in claim 1, is characterized in that: in step (1), describedThe addition of extractant is the extractant that adds 1L in the krill of every 1kg;
In step (1), after described mixing, the content of the alcohol in described mixture A is50%-70%; Described percentage is the percent by volume that described alcohol accounts for the liquid in described mixture A;
In step (3), the shrimp mud that the addition of described extractant is every 1kg adds the extractant of 1L.
5. preparation method as claimed in claim 1, is characterized in that: in step (1), describedThe speed of shearing is 10000rpm; The time of described shearing is 5-15min.
6. preparation method as claimed in claim 5, is characterized in that: the time of described shearing is10min; Described shearing is carried out in high-speed shearing machine.
7. preparation method as claimed in claim 1, is characterized in that: in step (2), describedThe separation factor of centrifugation is 2500-3000; The time of described centrifugation is 10-20min.
8. preparation method as claimed in claim 7, is characterized in that: described centrifugation timeBetween be 15min; Described centrifugation is for being used desk centrifuge or horizontal screw centrifuge centrifugal.
9. preparation method as claimed in claim 1, is characterized in that: in step (3), describedThe method of desolvation is vacuum removal solvent under the temperature conditions below 60 DEG C.
10. preparation method as claimed in claim 9, is characterized in that: the described temperature of deviating from solventDegree is 40-55 DEG C; The time of described desolvation is 10-30min.
11. preparation methods as described in claim 1-10 any one, is characterized in that:
In step (1), the concentration of the aqueous solution of described alcohol is more than 90%, and described percentage is instituteThe alcohol of stating accounts for the percent by volume of the aqueous solution of alcohol.
12. preparation methods as claimed in claim 11, is characterized in that: the aqueous solution of described alcoholConcentration be 95%.
13. preparation methods as described in claim 1-10 any one, is characterized in that:
In step (1), described drains as naturally draining;
The described time of thawing is in 30min; The consumption of described water is not have described freezing phosphorusShrimp.
14. preparation methods as described in claim 1-10 any one, is characterized in that: step (1)In, before described shearing, described krill and described extractant are stirred, and pulverize.
15. preparation methods as claimed in claim 14, is characterized in that: the method for described pulverizingPulverize for using gear pump.
16. preparation methods as described in claim 1-10 any one, is characterized in that:
In step (3), described being extracted as stirred and extracted; The time of described extraction is 20-40min;
In step (3), the number of times of described extraction is 2-3 time; When described extraction time is greater than 1When inferior, merge extract.
17. preparation methods as claimed in claim 16, is characterized in that: the time of described extractionFor 30min.
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| GB201400431D0 (en) * | 2014-01-10 | 2014-02-26 | Aker Biomarine As | Phospholipid compositions and their preparation |
| CN103981021A (en) * | 2014-05-31 | 2014-08-13 | 山东乾清翔泰生物制品有限公司 | Method for refining krill oil from Antarctic krill powder |
| KR20170118125A (en) | 2015-02-11 | 2017-10-24 | 에이커 바이오마린 앤탁틱 에이에스 | Lipid extraction process |
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| US4219470A (en) * | 1978-10-23 | 1980-08-26 | Dravo Corporation | Process for preparing a protein concentrate and the product obtained thereby |
| CN1269940C (en) * | 1998-10-21 | 2006-08-16 | 舍布鲁克大学 | Method for extracting lipid from marine and aquatic animal tissues |
| EP1331972B1 (en) * | 2000-09-11 | 2007-07-04 | Novartis AG | Pharmaceutical compositions |
| CN102899165A (en) * | 2012-10-25 | 2013-01-30 | 山东师范大学 | Technology for extracting bright red shrimp oil crude product from euphausia superba |
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| JP2010510208A (en) * | 2006-11-16 | 2010-04-02 | プロノヴァ バイオファーマ ノルゲ アーエス | Process for producing krill oil and meal |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4219470A (en) * | 1978-10-23 | 1980-08-26 | Dravo Corporation | Process for preparing a protein concentrate and the product obtained thereby |
| CN1269940C (en) * | 1998-10-21 | 2006-08-16 | 舍布鲁克大学 | Method for extracting lipid from marine and aquatic animal tissues |
| EP1331972B1 (en) * | 2000-09-11 | 2007-07-04 | Novartis AG | Pharmaceutical compositions |
| CN102899165A (en) * | 2012-10-25 | 2013-01-30 | 山东师范大学 | Technology for extracting bright red shrimp oil crude product from euphausia superba |
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