CN104688796A - Method for extracting maca bioactive components based on ultrasonic wave - Google Patents
Method for extracting maca bioactive components based on ultrasonic wave Download PDFInfo
- Publication number
- CN104688796A CN104688796A CN201510057855.2A CN201510057855A CN104688796A CN 104688796 A CN104688796 A CN 104688796A CN 201510057855 A CN201510057855 A CN 201510057855A CN 104688796 A CN104688796 A CN 104688796A
- Authority
- CN
- China
- Prior art keywords
- filtrate
- meyenii walp
- lepidinm meyenii
- container
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting maca bioactive components based on ultrasonic wave. The product containing high-concentration maca bioactive components is finally obtained by means of using a high-pressure wall-cracking technology and an ultrasonic wave extracting technology to process the maca root tuber. Compared with the current extracting technology, the method has the advantages that the bioactive components such as maca alkaloid, the macaenes and the macamides in the maca root tuber are effectively extracted, the raw material utilization rate is increased and the production cost is reduced. Furthermore, the product prepared according to the method has high bioactivity and much better healthcare effect than that of the prior art.
Description
Technical field
The present invention relates to the extracting method of Lepidinm meyenii Walp bioactive ingredients, be specifically related to a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients.
Background technology
Lepidinm meyenii Walp (Maca) has another name called Peruvian ginseng, maka etc., the annual or 2 years raw herbaceous plant in South America, belong to Cruciferae (Cruciferae) separate row Vegetable spp (Lepidium L), concrete kind is also disputable, generally believe in the world, Lepidium meyenii Walp and Lepidium peruvianum Chacon two kinds almost do not have difference on growth and breeding, phytochemistry and health-care effect, are therefore collectively referred to as " Maca " (transliteration is " Lepidinm meyenii Walp ").Lepidinm meyenii Walp has had the cultivation history of several thousand in mountain area, Andean, South America. and be one of indispensable crop.There is good health-care effect.
Lepidinm meyenii Walp alkaloid can improve the quantity of the quantity of animal mature follicle corpusculum, the mobility of sperm and sperm, thus can significantly improve the fertility of mammal and Fish.And the alkaloid in Lepidinm meyenii Walp can act on hypothalamus and hypophysis cerebri, there is endocrine regulation gland as functions such as adrenal gland, thyroid, pancreas, ovaries, thus balance hormone, thus can with giving treatment female climacteric syndrome.
Common alkaloid extracting method has sour water direct extraction method, infusion process, oozes rumble method, hot cocurrent flow method, supersound extraction etc.At present, only have about the alkaloidal supersound extraction of Lepidinm meyenii Walp.Therefore, adopt conventional plant supercritical ultrasonics technology process Lepidinm meyenii Walp, the alkaloidal productive rate of Lepidinm meyenii Walp is lower, causes that existing Lepidinm meyenii Walp alkaloid cost is high and health-care effect is not obvious.
The ultrasound wave of prior art is lower to Maca reactive constituents extraction efficiency, and mainly because the wall mechanical intensity of Lepidinm meyenii Walp tuber cell is higher, the mechanical activation comminution breaking cellular wall efficiency of general cutting type is low, causes a large amount of cell inclusions to be intercepted by cell wall.First the present invention applies pressure-air to granule, and again at abrupt release pressure, make individual cells instantaneous expansion and cell wall is burst, cellular content ejects in a large number.Prior art has employing to be filled with ammonia to make raw material inflated with low pressure at a higher temperature and realize shell-broken effect, but for Lepidinm meyenii Walp raw material, high temperature likely makes active substance distortion wherein, also likely makes wherein volatile Lepidinm meyenii Walp alkene, Semen Sinapis wet goods substance escaping, loss.After tested, pass into the unstability that ammonia contributes to improving cell wall, it is more violent that the expansion made it bursts process, also contributes to the volatilization efficiency reducing Lepidinm meyenii Walp alkene, mustard oil, improve the productive rate of Lepidinm meyenii Walp alkene and Semen Sinapis wet goods.Sodium citrate can ensure breaking cellular wall product stand ammonia corrosion after stability, especially can avoid reactive protein inactivation wherein.Described sodium citrate-alcohol mixture is the mixture of the sodium citrate of 2 ~ 3 parts by weight and the ethanol of 60 ~ 90 parts.Grain (partly) footpath is that the granule of 0.2 ~ 0.5mm is easy to processing by prior art, and more cellulous cell wall can be made to expose in atmosphere.Described container can select the steam-explosion jar of prior art.Ultrasonic extraction in the present invention can select any one ultrasonic extraction equipment to realize, and common ultrasonic extraction also can be adopted to realize.Utilize repeated ultrasonic ripple to extract, obviously can promote the Maca reactive composition especially alkaloidal productive rate of Lepidinm meyenii Walp.Because the activity component concentration in different extraction time materials is different, sets different extraction conditions, can obtain the highest productive rate under minimum cost through multiple authentication the present invention, product has the highest biological activity simultaneously.Especially, the high pressure limit in container is decided to be 0.5 ~ 0.7Mpa of gauge pressure by the present invention.Upon discharge, volatile Lepidinm meyenii Walp alkene is by the loss of pressure along with release, and too low pressure cannot thorough fracturing cell walls for too high pressure.But in the present invention, the cell wall through ammonia process is easy to broken, thus is minimized the pressure in container, prevents the loss of volatile substances.The input quantity of ammonia can be preferably 1.3 ~ 2.5 times of vessel volume.
Further, Aging storage in described S6 adds 1 ~ 6 part of Aloe root system suspension in the filtrate pointing to 50 ~ 70 parts by weight, add 2 ~ 8 parts of vitamin Cs, 10 ~ 17 parts of glucoses, 2 ~ 15 parts of ethanol simultaneously, in sealed container, within 5th ~ 21, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 35 ~ 45 DEG C of anoxybiotic process.After-ripening step can improve the biological activity of product, improves the Lepidinm meyenii Walp aromatic odor in product.The secretions of Aloe root system and microorganism contribute to the carrying out of after-ripening step.
Further, described Aloe root system suspension is the Radix of Aloe of 10 ~ 19 parts by weight, pulverizing is that the segment of 1 ~ 3mm is immersed in 20 ~ 40 parts of sterilized water and 2 ~ 15 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 200 ~ 250 turns/min.
Radix of Aloe be cut into segment and adopt bead to extrude, contributing to the release of above-mentioned secretions.
Described Radix of Aloe is the fresh aloe root of prior art, and its weight is fresh weight.
Particularly preferred, described operation S1 pre-treatment Lepidinm meyenii Walp tuber refers to and carries out 5 brittle operations of low temperature cell wall to Lepidinm meyenii Walp tuber; The brittle operation of described low temperature cell wall is specially is down to-9 ~-5 DEG C by the temperature of Lepidinm meyenii Walp tuber in 60s, after maintenance 30min, the mass fraction be immersed in containing 30 ~ 40 DEG C of constant temperature is 60min in the sodium chloride solution of 0.01%, and it is the acetic acid solution of 6% that every kilogram of Lepidinm meyenii Walp tuber sprays 3 ~ 6g mass fraction.
Under cryogenic, intracellular Free water will condense into trickle ice crystal and puncture cell membrane and cell wall.And without the materials such as cholesterol plant cell membrane at low temperatures mobility decline, brittle, easily broken.Especially, after acetic acid and sodium chloride process, also and easily damaged under low temperature repeatedly, the stress making cell wall occur that slight crack fills bears some the cell wall of Lepidinm meyenii Walp, is conducive to the thorough fragmentation of cell wall in follow-up breaking cellular wall operation.
Summary of the invention
In view of this, the present invention discloses a kind of extraction process that significantly can improve Lepidinm meyenii Walp alkaloid productive rate, reduce Maca reactive composition production cost, strengthen Lepidinm meyenii Walp bioactive ingredients physiological function.
Object of the present invention is achieved through the following technical solutions:
Based on the method for ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprise following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. described Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to described granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 90 ~ 150min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix described first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Pre-treatment alleged by S1 comprises the conventional pre-treatment step of prior art, as clean, dry etc.Lepidinm meyenii Walp tuber alleged by the present invention is the commercially available prod of water content at 50wt% ~ 75wt%.S2 can adopt any one existing techniques in realizing, as existing beater grinder.Pulverize raw-material tuber for granule, be conducive to the specific surface area increasing raw material, in follow-up broken wall treatment, having larger area bears the rising of pressure and reduces instantaneously, thus improves breaking cellular wall efficiency.Through the raw material of broken wall treatment, the Lepidinm meyenii Walp alkaloid of ultrasonic extraction, macamide, activated protein productive rate are far above the ultrasonic extraction of prior art.And the filtering residue after ultrasonic extraction uses hydro-thermal method lixiviate further, active component remaining in filtering residue thoroughly can be extracted under lower-cost prerequisite, improve the utilization rate of raw material, waste can be reduced and effectively control the production cost of Maca reactive composition in large-scale production and application.Aging storage can be leave standstill, lucifuge preservation 20 ~ 30 days.Broken wall treatment also can adopt any one plant wall breaking technology to realize.
Further, described broken wall treatment refers to the container described granule being placed in sealing, passes into the ammonia equal with vessel volume, and heating container maintains 3 ~ 5min to container Nei Wenduda 60 ~ 65 DEG C; Stop ammonia, and be 0.5 ~ 0.7Mpa to inputting gauge pressure in pressure-air to container in container and maintain 60 ~ 130 seconds, in 0.5 ~ 1 second, the gauge pressure in container is reduced to 0pa; Adopt volume to be vessel volume 40% ~ 80% sodium citrate and alcohol mixture eluant container, collect flushing thing and be described breaking cellular wall product;
The particle diameter of described granule is 0.2 ~ 0.5mm;
Further, adopt ultrasonic extraction process breaking cellular wall product to refer to described breaking cellular wall product and quality to be that its ethanol of 2 ~ 5 times mixes, after being heated to 30 ~ 40 DEG C, adopt the ultrasonic extraction 30 ~ 50min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Described multiple extract refer to adopt quality be the ethanol of filtering residue 3 ~ 5 times at 50 ~ 80 DEG C with the ultrasonic extraction 90 ~ 140min of 60KHz; Merging filtrate obtains described first filtrate.
Detailed description of the invention
The present invention to be described in further detail below in conjunction with embodiment for the ease of it will be appreciated by those skilled in the art that:
Embodiment 1
The present embodiment provides a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprises following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. above-mentioned Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to above-mentioned granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 125min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix above-mentioned first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Above-mentioned broken wall treatment refers to the container above-mentioned granule being placed in sealing, passes into ammonia, and heating container maintains 4min to container Nei Wenduda 60 DEG C; Stop input ammonia, and be 0.6Mpa to inputting gauge pressure in pressure-air to container in container and maintain 90 seconds, in 0.5 second, the gauge pressure in container is reduced to 0pa; Adopt alcohol flushing container, collect flushing thing and be above-mentioned breaking cellular wall product;
The particle diameter of above-mentioned granule is 0.4mm;
Adopt ultrasonic extraction process breaking cellular wall product to refer to above-mentioned breaking cellular wall product and quality to be that its ethanol of 3 times mixes, after being heated to 35 DEG C, adopt the ultrasonic extraction 45min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Above-mentioned multiple extract refer to adopt quality be the ethanol of filtering residue 4 times at 60 DEG C with the ultrasonic extraction 100min of 60KHz; Merging filtrate obtains above-mentioned first filtrate.
Aging storage in above-mentioned S6 adds 3 parts of Aloe root system suspensions in the filtrate pointing to 55 parts by weight, add 7 parts of vitamin Cs, 12 parts of glucoses, 9 parts of ethanol simultaneously, in sealed container, within 14th, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 40 DEG C of anoxybiotic process.
Above-mentioned Aloe root system suspension is the Radix of Aloe of 17 parts by weight, and the segment pulverized as 3mm is immersed in 30 parts of sterilized water and 8 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 250 turns/min.Above-mentioned operation S1 pre-treatment Lepidinm meyenii Walp tuber refers to and carries out 5 brittle operations of low temperature cell wall to Lepidinm meyenii Walp tuber; The brittle operation of above-mentioned low temperature cell wall is specially is down to-9 ~-5 DEG C by the temperature of Lepidinm meyenii Walp tuber in 60s, after maintenance 30min, the mass fraction be immersed in containing 30 ~ 40 DEG C of constant temperature is 60min in the sodium chloride solution of 0.01%, and it is the acetic acid solution of 6% that every kilogram of Lepidinm meyenii Walp tuber sprays 3 ~ 6g mass fraction.
In the present embodiment, 1 part is 1kg.Adopt the Lepidinm meyenii Walp tuber (water content 70wt%) of 60 parts, through the concentrated product finally preparing 73.25kg, wherein Lepidinm meyenii Walp alkaloid is 327g after measured, and Lepidinm meyenii Walp alkene content is 109g, and macamide is 0.999g, polysaccharide 538.39g, glucosinolate 981g.
Embodiment 2
The present embodiment provides a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprises following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. above-mentioned Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to above-mentioned granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 150min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix above-mentioned first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Above-mentioned broken wall treatment refers to the container above-mentioned granule being placed in sealing, passes into ammonia, and heating container maintains 5min to container Nei Wenduda 60 DEG C; Stop input ammonia, and be 0.5Mpa to inputting gauge pressure in pressure-air to container in container and maintain 130 seconds, in 0.5 second, the gauge pressure in container is reduced to 0pa; Adopt alcohol flushing container, collect flushing thing and be above-mentioned breaking cellular wall product;
The particle diameter of above-mentioned granule is 0.5mm;
Adopt ultrasonic extraction process breaking cellular wall product to refer to above-mentioned breaking cellular wall product and quality to be that its ethanol of 2 times mixes, after being heated to 40 DEG C, adopt the ultrasonic extraction 30min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Above-mentioned multiple extract refer to adopt quality be the ethanol of filtering residue 5 times at 50 DEG C with the ultrasonic extraction 140min of 60KHz; Merging filtrate obtains above-mentioned first filtrate.
Aging storage in above-mentioned S6 adds 6 parts of Aloe root system suspensions in the filtrate pointing to 50 parts by weight, add 2 parts of vitamin Cs, 17 parts of glucoses, 2 parts of ethanol simultaneously, in sealed container, within 5th, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 45 DEG C of anoxybiotic process.
Above-mentioned Aloe root system suspension is the Radix of Aloe of 19 parts by weight, and the segment pulverized as 1mm is immersed in 40 parts of sterilized water and 2 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 250 turns/min.
In the present embodiment, 1 part is 1kg.Adopt the Lepidinm meyenii Walp tuber (water content 70wt%) of 60 parts, through the concentrated product finally preparing 75.69kg, wherein Lepidinm meyenii Walp alkaloid (total alkali after measured, acetic acid titration) content is 277.20g, Lepidinm meyenii Walp alkene content is 98.11g, macamide is 1.237g, polysaccharide 529.82g, glucosinolate 997.83g.
Embodiment 3
The present embodiment provides a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprises following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. above-mentioned Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to above-mentioned granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 90min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix above-mentioned first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Above-mentioned broken wall treatment refers to the container above-mentioned granule being placed in sealing, passes into ammonia, and heating container maintains 3min to container Nei Wenduda 65 DEG C; Stop input ammonia, and be 0.7Mpa to inputting gauge pressure in pressure-air to container in container and maintain 60 seconds, in 1 second, the gauge pressure in container is reduced to 0pa; Adopt alcohol flushing container, collect flushing thing and be above-mentioned breaking cellular wall product;
The particle diameter of above-mentioned granule is 0.2mm;
Adopt ultrasonic extraction process breaking cellular wall product to refer to above-mentioned breaking cellular wall product and quality to be that its ethanol of 5 times mixes, after being heated to 30 DEG C, adopt the ultrasonic extraction 50min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Above-mentioned multiple extract refer to adopt quality be the ethanol of filtering residue 3 times at 80 DEG C with the ultrasonic extraction 90min of 60KHz; Merging filtrate obtains above-mentioned first filtrate.
Aging storage in above-mentioned S6 adds 1 part of Aloe root system suspension in the filtrate pointing to 70 parts by weight, add 8 parts of vitamin Cs, 10 parts of glucoses, 15 parts of ethanol simultaneously, in sealed container, within 21st, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 35 DEG C of anoxybiotic process.
Above-mentioned Aloe root system suspension is the Radix of Aloe of 10 parts by weight, and the segment pulverized as 3mm is immersed in 20 parts of sterilized water and 15 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 200 turns/min.
In the present embodiment, 1 part is 1kg.Adopt the Lepidinm meyenii Walp tuber (water content 70wt%) of 60 parts, through the concentrated product finally preparing 74.75kg, wherein Lepidinm meyenii Walp alkaloid is 309g after measured, and Lepidinm meyenii Walp alkene content is 87g, and macamide is 0.952g, polysaccharide 511.47g, glucosinolate 961g.
Embodiment 4
The present embodiment provides a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprises following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. above-mentioned Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to above-mentioned granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 125min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix above-mentioned first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Above-mentioned pre-treatment is carry out conventional cleaning, be dried to water content being 70wt% to fresh Lepidinm meyenii Walp tuber.Above-mentioned broken wall treatment refers to the container above-mentioned granule being placed in sealing, passes into ammonia, and heating container maintains 4min to container Nei Wenduda 60 DEG C; Stop input ammonia, and be 0.6Mpa to inputting gauge pressure in pressure-air to container in container and maintain 90 seconds, in 0.5 second, the gauge pressure in container is reduced to 0pa; Adopt alcohol flushing container, collect flushing thing and be above-mentioned breaking cellular wall product;
The particle diameter of above-mentioned granule is 0.4mm;
Adopt ultrasonic extraction process breaking cellular wall product to refer to above-mentioned breaking cellular wall product and quality to be that its ethanol of 3 times mixes, after being heated to 35 DEG C, adopt the ultrasonic extraction 45min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Above-mentioned multiple extract refer to adopt quality be the ethanol of filtering residue 4 times at 60 DEG C with the ultrasonic extraction 100min of 60KHz; Merging filtrate obtains above-mentioned first filtrate.
In the present embodiment, 1 part is 1kg.Adopt the Lepidinm meyenii Walp tuber (water content 70wt%) of 60 parts, through the concentrated product finally preparing 73.25kg, wherein Lepidinm meyenii Walp alkaloid is 265g after measured, and Lepidinm meyenii Walp alkene content is 65g, and macamide is 0.907g, polysaccharide 458.39g, glucosinolate 710g.
Embodiment 5
The present embodiment provides a kind of method based on ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprises following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. above-mentioned Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to above-mentioned granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 125min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix above-mentioned first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
Above-mentioned broken wall treatment is that pressure-air is pulverized.
Aging storage in above-mentioned S6 adds 3 parts of Aloe root system suspensions in the filtrate pointing to 55 parts by weight, add 7 parts of vitamin Cs, 12 parts of glucoses, 9 parts of ethanol simultaneously, in sealed container, within 14th, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 40 DEG C of anoxybiotic process.
Above-mentioned Aloe root system suspension is the Radix of Aloe of 17 parts by weight, and the segment pulverized as 3mm is immersed in 30 parts of sterilized water and 8 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 250 turns/min.
Adult male mice (body weight 113.11 ± 5.72g) is divided into 6 groups, often organizes 50, often group gavage feeding embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and normal saline respectively, feeding dosage is 0.5g.Feeding process lasts 50 days.Mice takes food food and water since the childhood.
Often group chooses 30 mices, orders about its swimming, test mouse blood sugar concentration after 120min in being gone in off the deep end.And test the rate of increase of Mouse Weight in 50 days.Its result is as shown in table 1.
Table 1 mouse test results
Be more than wherein specific implementation of the present invention, it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these apparent replacement forms all belong to protection scope of the present invention.
Claims (5)
1., based on a method for ultrasonic extraction Lepidinm meyenii Walp bioactive ingredients, comprise following operation:
S1. pre-treatment Lepidinm meyenii Walp tuber;
S2. described Lepidinm meyenii Walp tuber is pulverized for granule;
S3. broken wall treatment is carried out to described granule, obtain breaking cellular wall product;
S4. ultrasonic extraction process breaking cellular wall product is adopted; Collecting by filtration filtrate, obtains the first filtrate;
S5. collect filtering residue, and adopt purified water lixiviate 90 ~ 150min, collecting by filtration filtrate, obtain the second filtrate;
S6. mix described first filtrate and the second filtrate, obtain Lepidinm meyenii Walp bioactive ingredients product through Aging storage.
2. method according to claim 1, is characterized in that: described broken wall treatment refers to the container described granule being placed in sealing, passes into ammonia, and heating container maintains 3 ~ 5min to container Nei Wenduda 60 ~ 65 DEG C; Stop input ammonia, and be 0.5 ~ 0.7Mpa to inputting gauge pressure in pressure-air to container in container and maintain 60 ~ 130 seconds, in 0.5 ~ 1 second, the gauge pressure in container is reduced to 0pa; Adopt alcohol flushing container, collect flushing thing and be described breaking cellular wall product;
The particle diameter of described granule is 0.2 ~ 0.5mm;
Adopt ultrasonic extraction process breaking cellular wall product to refer to described breaking cellular wall product and quality to be that its ethanol of 2 ~ 5 times mixes, after being heated to 30 ~ 40 DEG C, adopt the ultrasonic extraction 30 ~ 50min of 80KHz, after filtration, three multiple extractions are carried out to filtering residue; Described multiple extract refer to adopt quality be the ethanol of filtering residue 3 ~ 5 times at 50 ~ 80 DEG C with the ultrasonic extraction 90 ~ 140min of 60KHz; Merging filtrate obtains described first filtrate.
3. method according to claim 1, it is characterized in that: the Aging storage in described S6 adds 1 ~ 6 part of Aloe root system suspension in the filtrate pointing to 50 ~ 70 parts by weight, add 2 ~ 8 parts of vitamin Cs, 10 ~ 17 parts of glucoses, 2 ~ 15 parts of ethanol simultaneously, in sealed container, within 5th ~ 21, obtain Lepidinm meyenii Walp bioactive ingredients product afterwards with 35 ~ 45 DEG C of anoxybiotic process.
4. method according to claim 3, it is characterized in that: described Aloe root system suspension is the Radix of Aloe of 10 ~ 19 parts by weight, pulverizing is that the segment of 1 ~ 3mm is immersed in 20 ~ 40 parts of sterilized water and 2 ~ 15 parts of particle diameters are in the bead of 1mm, filters the filtrate of acquisition after adopting rotary shaker to vibrate 6 minutes under the speed of 200 ~ 250 turns/min.
5. method according to claim 1, is characterized in that: described operation S1 pre-treatment Lepidinm meyenii Walp tuber refers to and carries out 5 brittle operations of low temperature cell wall to Lepidinm meyenii Walp tuber; The brittle operation of described low temperature cell wall is specially is down to-9 ~-5 DEG C by the temperature of Lepidinm meyenii Walp tuber in 60s, after maintenance 30min, the mass fraction be immersed in containing 30 ~ 40 DEG C of constant temperature is 60min in the sodium chloride solution of 0.01%, and it is the acetic acid solution of 6% that every kilogram of Lepidinm meyenii Walp tuber sprays 3 ~ 6g mass fraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510057855.2A CN104688796B (en) | 2015-02-04 | 2015-02-04 | Method based on ultrasonic wave extraction maca bioactive ingredients |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510057855.2A CN104688796B (en) | 2015-02-04 | 2015-02-04 | Method based on ultrasonic wave extraction maca bioactive ingredients |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104688796A true CN104688796A (en) | 2015-06-10 |
| CN104688796B CN104688796B (en) | 2017-11-17 |
Family
ID=53336620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510057855.2A Active CN104688796B (en) | 2015-02-04 | 2015-02-04 | Method based on ultrasonic wave extraction maca bioactive ingredients |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104688796B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146511A (en) * | 2015-07-31 | 2015-12-16 | 昆明博尚生物技术有限公司 | Processing method for improving specific active components of Peruvian ginseng |
| CN105941819A (en) * | 2016-04-27 | 2016-09-21 | 云南还原医学生物科技有限公司 | Extraction method of maca crude proteins |
| CN106723001A (en) * | 2017-01-13 | 2017-05-31 | 中国科学院过程工程研究所 | The purposes of the method for amide content and obtained product and product in a kind of raising maca |
| CN107982439A (en) * | 2017-12-22 | 2018-05-04 | 营养屋(成都)生物医药有限公司 | The extracting method of dendrobium candidum American ginseng capsule, its preparation method and dendrobium candidum |
| CN108685969A (en) * | 2018-06-11 | 2018-10-23 | 南方医科大学 | A kind of extracting method of maca total alkaloid |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526161A (en) * | 2012-02-10 | 2012-07-04 | 中国科学院过程工程研究所 | Preparation method for high-activity lepidium meyenii extract |
| CN102763742A (en) * | 2012-07-25 | 2012-11-07 | 北京中科健宇生物科技有限公司 | Maca instant tea and production method thereof |
| CN103751250A (en) * | 2014-01-14 | 2014-04-30 | 云南和融生物科技有限公司 | Method for extracting maca alkaloids through microwave and ultrasonic wave combination |
| CN104127468A (en) * | 2014-07-30 | 2014-11-05 | 烟台新时代健康产业有限公司 | Preparation extraction process of Maca extract |
| CN104146250A (en) * | 2014-08-02 | 2014-11-19 | 昆明骊玛生物科技有限公司 | Preparation method of Maca composition and application of preparation method |
-
2015
- 2015-02-04 CN CN201510057855.2A patent/CN104688796B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526161A (en) * | 2012-02-10 | 2012-07-04 | 中国科学院过程工程研究所 | Preparation method for high-activity lepidium meyenii extract |
| CN102763742A (en) * | 2012-07-25 | 2012-11-07 | 北京中科健宇生物科技有限公司 | Maca instant tea and production method thereof |
| CN103751250A (en) * | 2014-01-14 | 2014-04-30 | 云南和融生物科技有限公司 | Method for extracting maca alkaloids through microwave and ultrasonic wave combination |
| CN104127468A (en) * | 2014-07-30 | 2014-11-05 | 烟台新时代健康产业有限公司 | Preparation extraction process of Maca extract |
| CN104146250A (en) * | 2014-08-02 | 2014-11-19 | 昆明骊玛生物科技有限公司 | Preparation method of Maca composition and application of preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 杜广香 等: "超声波提取玛咖生物碱的工艺研究", 《广东农业科学》 * |
| 梁邢文 等: "《饲料原料与品质检测》", 31 August 1999 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146511A (en) * | 2015-07-31 | 2015-12-16 | 昆明博尚生物技术有限公司 | Processing method for improving specific active components of Peruvian ginseng |
| CN105941819A (en) * | 2016-04-27 | 2016-09-21 | 云南还原医学生物科技有限公司 | Extraction method of maca crude proteins |
| CN106723001A (en) * | 2017-01-13 | 2017-05-31 | 中国科学院过程工程研究所 | The purposes of the method for amide content and obtained product and product in a kind of raising maca |
| CN106723001B (en) * | 2017-01-13 | 2021-01-22 | 中国科学院过程工程研究所 | Method for increasing content of amide in maca, prepared product and application of product |
| CN107982439A (en) * | 2017-12-22 | 2018-05-04 | 营养屋(成都)生物医药有限公司 | The extracting method of dendrobium candidum American ginseng capsule, its preparation method and dendrobium candidum |
| CN107982439B (en) * | 2017-12-22 | 2020-09-29 | 营养屋(成都)生物医药有限公司 | Dendrobium officinale and American ginseng capsule, preparation method thereof and extraction method of dendrobium officinale |
| CN108685969A (en) * | 2018-06-11 | 2018-10-23 | 南方医科大学 | A kind of extracting method of maca total alkaloid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104688796B (en) | 2017-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104688796A (en) | Method for extracting maca bioactive components based on ultrasonic wave | |
| CN101986879B (en) | Manufacturing method for enzyme-containing healthy food and healthy food | |
| CN105380246A (en) | Bone flavored Qi-regulating fresh and wet sweet potato vermicelli and preparation method thereof | |
| CN102895281A (en) | Method for extracting active ingredient from maca | |
| CN105168285A (en) | Ginsenoside extracting solution and extracting method thereof | |
| CN104256721A (en) | Fruit-vinegar dried silver fish and preparation method thereof | |
| CN106579443A (en) | Method for extracting anthocyanin from mulberries | |
| CN101502316A (en) | Method for extracting medlar polysaccharide | |
| CN103694205A (en) | Method for extracting Vc from sea buckthorn | |
| CN103767019B (en) | Process for extracting original liquid from fresh momordica grosvenori through low-temperature immersion reverse flow method | |
| CN103951713A (en) | Comprehensive utilization method for improving value of cucumis sativus seed medicinal materials | |
| CN101485429B (en) | Preparation method of hypoglycemic bitter melon freeze-dried powder | |
| CN105341825A (en) | Lycium ruthenicum fruit and haw jam and preparation method thereof | |
| CN107821924A (en) | A kind of extraction process of garnet pigment | |
| CN106632208A (en) | Extraction method for anthocyanin from purple sweet potato | |
| CN109757642A (en) | A kind of preparation method of balsam pear solid beverage | |
| CN102273517A (en) | Trichosanthes kirilowii Maxim seed plant protein beverage and production method thereof | |
| CN104365941A (en) | Technology for processing clove and bitter gourd health tea | |
| CN108175032A (en) | A kind of black rice enzymolysis liquid is prepared and black rice pigments extracting method | |
| CN105998750A (en) | Avicennia marina fruit wine | |
| CN105795479A (en) | Maca extract, preparation method thereof and method for preparing enzyme beverages from maca extract | |
| CN102273628A (en) | Pure cactus powder and preparation method thereof | |
| CN105146630A (en) | Preparation method of active Korla bergamot pear juice | |
| CN108451996A (en) | A method of preparing maca extract | |
| CN107319483A (en) | A kind of green All Pure Nature richness dimension pregnant precious salt of comprehensive nutrient type and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liang Nan Inventor after: Huang Minxin Inventor after: Lin Chuxiong Inventor after: Liu Yingmin Inventor after: He Caixia Inventor after: Yang Likai Inventor after: Li Guowei Inventor before: Liang Nan Inventor before: Lin Chuxiong Inventor before: Liu Yingmin Inventor before: He Caixia Inventor before: Yang Likai Inventor before: Li Guowei |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |