CN104662170A - 通过使用流式细胞术的rna测量快速检测t细胞活化 - Google Patents

通过使用流式细胞术的rna测量快速检测t细胞活化 Download PDF

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Publication number
CN104662170A
CN104662170A CN201380045036.5A CN201380045036A CN104662170A CN 104662170 A CN104662170 A CN 104662170A CN 201380045036 A CN201380045036 A CN 201380045036A CN 104662170 A CN104662170 A CN 104662170A
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China
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cell
cells
probe
cytokine
peptide
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CN201380045036.5A
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English (en)
Chinese (zh)
Inventor
Y·布希金
M·L·真纳罗
桑杰·泰尔吉
理查德·皮纳
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Rutgers State University of New Jersey
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Rutgers State University of New Jersey
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Priority to CN201810454480.7A priority Critical patent/CN108624654A/zh
Publication of CN104662170A publication Critical patent/CN104662170A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6866—Interferon
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380045036.5A 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化 Pending CN104662170A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810454480.7A CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261665231P 2012-06-27 2012-06-27
US61/665,231 2012-06-27
US201361784802P 2013-03-14 2013-03-14
US61/784,802 2013-03-14
PCT/US2013/047774 WO2014004609A2 (en) 2012-06-27 2013-06-26 Rapid assays for t-cell activation by rna measurements using flow cytometry

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810454480.7A Division CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

Publications (1)

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CN104662170A true CN104662170A (zh) 2015-05-27

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CN201380045036.5A Pending CN104662170A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化
CN201810454480.7A Pending CN108624654A (zh) 2012-06-27 2013-06-26 通过使用流式细胞术的rna测量快速检测t细胞活化

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US (2) US10900072B2 (enExample)
EP (2) EP2867374B1 (enExample)
JP (1) JP6464081B2 (enExample)
KR (1) KR20150036186A (enExample)
CN (2) CN104662170A (enExample)
AU (2) AU2013280416B2 (enExample)
BR (1) BR112014032677A2 (enExample)
CA (1) CA2877933A1 (enExample)
IN (1) IN2015KN00073A (enExample)
RU (1) RU2671707C2 (enExample)
WO (1) WO2014004609A2 (enExample)
ZA (1) ZA201500239B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法
CN111999235A (zh) * 2020-07-03 2020-11-27 青岛农业大学 一种利用流式细胞术快速检测有活力的食用菌原生质体数量的方法

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BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
CN106198358B (zh) * 2016-06-24 2019-02-15 北京大学 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7457572B2 (ja) * 2019-05-27 2024-03-28 住友化学株式会社 光学フィルムの反りの測定方法、及び、測定装置
EP4025595A4 (en) * 2019-09-03 2023-11-29 The Regents Of The University Of California METHOD FOR SEQUENCE OF MRNA IN PARALLEL SINGLE CELLS WITH QUANTIFICATION OF INTRACELLULAR PHENOTYPE
JP7784139B2 (ja) * 2019-10-29 2025-12-11 ウニベルジテート ハイデルベルク 人工抗原提示分子とその利用
RU2766781C1 (ru) * 2021-03-03 2022-03-15 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр кардиологии" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ кардиологии" Минздрава России) Способ выявления поражения сосудистого эндотелия
WO2022271272A1 (en) * 2021-06-22 2022-12-29 Nantcell, Inc. Rapid test for patient cd8 t-cell activation, systems and methods

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法
CN111999235A (zh) * 2020-07-03 2020-11-27 青岛农业大学 一种利用流式细胞术快速检测有活力的食用菌原生质体数量的方法

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EP2867374A2 (en) 2015-05-06
AU2013280416B2 (en) 2017-06-15
AU2017228677A1 (en) 2017-10-05
CN108624654A (zh) 2018-10-09
WO2014004609A2 (en) 2014-01-03
US20160201122A1 (en) 2016-07-14
BR112014032677A2 (pt) 2019-01-15
EP3828278A1 (en) 2021-06-02
RU2015102447A (ru) 2016-08-20
EP2867374B1 (en) 2020-10-28
AU2013280416A1 (en) 2015-02-05
JP6464081B2 (ja) 2019-02-06
WO2014004609A3 (en) 2014-02-27
US10900072B2 (en) 2021-01-26
IN2015KN00073A (enExample) 2015-07-31
EP2867374A4 (en) 2016-05-11
US11767551B2 (en) 2023-09-26
KR20150036186A (ko) 2015-04-07
RU2671707C2 (ru) 2018-11-06
CA2877933A1 (en) 2014-01-03
AU2017228677B2 (en) 2019-02-14
US20210139965A1 (en) 2021-05-13
ZA201500239B (en) 2015-12-23
JP2015522284A (ja) 2015-08-06

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Application publication date: 20150527