CN104651256A - Mannitol-yolk medium and use thereof - Google Patents
Mannitol-yolk medium and use thereof Download PDFInfo
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- CN104651256A CN104651256A CN201310599781.6A CN201310599781A CN104651256A CN 104651256 A CN104651256 A CN 104651256A CN 201310599781 A CN201310599781 A CN 201310599781A CN 104651256 A CN104651256 A CN 104651256A
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- mannitol
- yolk
- yolk medium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention belongs to the field of microbes and relates to a mannitol-yolk medium and a use thereof. The mannitol-yolk medium comprises 20.1g/L of common salt broth with a concentration of 7.5%, 8.0g/L of D-mannitol, 0.032g/L of phenol red, 0.5g/L of potassium dihydrogen phosphate and 65.2g/L of a yolk emulsion. The mannitol-yolk medium has a pH value of 7.2-7.6. Each liter of the common salt broth with a concentration of 7.5% comprises 10.0g of peptone, 5.0g of beef powder, 75.0g of common salt and 2.0g of dipotassium hydrogen phosphate. The mannitol-yolk medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of N.F,USP MANNITOL egg yolk medium and application thereof.
background technology
Streptococcus aureus, also claims " S. aureus L-forms ", and cell walls is containing the peptidoglycan of 90% and the teichoic acid of 10%.The reticulated structure of its peptidoglycan is finer and close than Gram-negative bacteria, do not decoloured by alcohol so present purple after Viola crystallina attachment during dyeing, on the contrary, the whole cell peptidoglycan layer of negative bacterium is thin, degree of crosslinking is poor, lipid content is high, so purple mixture is washed out by alcohol then attached to husky yellow redness.Streptococcus aureus and penicillin find that there is very large origin.Fleming is exactly in the culture dish of his streptococcus aureus, find that some coccus has been killed then, so found penicillin.And research also shows that penicillin is only obvious to the gram-positive microorganism effect taking streptococcus aureus as representative.This is also caused by the thickness of peptidoglycan layer and structure.Emerging methicillin-resistant staphylococcus aureus, is referred to as superbacteria, almost can resist all medicines of the mankind, but vancomycin can tackle it.Typical streptococcus aureus is ball-type, and diameter about 0.8 μm, is arranged in thyrsiform under microscope.
Streptococcus aureus is without brood cell, flagellum, most of without pod membrane, Gram-positive.Streptococcus aureus nutritional requirement is not high, well-grown on ordinary culture medium, aerobic or amphimicrobian, and optimum growth temperature 37 ° of C, the most suitable growth pH7.4, can viable for weeks under dry environment.Thick, glossy, the circular protrusions of bacterium colony on flat board, diameter 0.5 ~ 1.0mm.Blood agar periphery of bacterial colonies forms transparent zone of hemolysis.Streptococcus aureus has the salt tolerance of height, can grow in 10 ~ 15%NaCl meat soup.Decomposable asymmetric choice net glucose, maltose, lactose, sucrose, produce acid not aerogenesis.Clark and Lubsreaction is positive, and VP reacts the weak positive.Many bacterial strain decomposable asymmetric choice net arginine, hydrolyze urea, reduction nitrate, liquefy gelatin.Streptococcus aureus has stronger resistibility, to antibiotic sensitive such as sulfa drugs, penicillin, erythromycin, terramycin, Liu Suanyan NEOMYCIN SULPHATEs, but easily produces resistance, and reason is because these bacterial strains produce penicillinase etc.Responsive to basic dyestuff, 100,000/ Viola crystallina liquid get final product Developing restraint.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of N.F,USP MANNITOL egg yolk medium and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of N.F,USP MANNITOL egg yolk medium, the component of described substratum comprises that 20.1g/L 7.5% sodium chloride broth, 8.0g/L PEARLITOL 25C, 0.032g/L are phenol red, 0.5g/L potassium primary phosphate, 65.2g/L yolk emulsion, and pH value is 7.2-7.6;
On the basis of above scheme, described 7.5% sodium chloride broth is containing Tryptones 10.0g, beef powder 5.0g, sodium-chlor 75.0g, dipotassium hydrogen phosphate 2.0g in often liter of 7.5% sodium chloride broth.
The invention also discloses the application of described N.F,USP MANNITOL egg yolk medium, the selective separation for streptococcus aureus is cultivated.
Peptone and beef powder provide nitrogenous source, VITAMIN and somatomedin; D-N.F,USP MANNITOL is fermentable saccharide; Sodium-chlor maintains balanced osmotic pressure; Agar is the peptizer of substratum; Phenol red is pH indicator.
Take this product 112.5g, be dissolved in 1000ml distilled water, boil 1 minute, 121 DEG C of autoclavings 15 minutes, when being cooled to 45-50 DEG C, aseptic technique adds yolk emulsion 3, shakes up, impouring sterilized petri dishes.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of N.F,USP MANNITOL egg yolk medium, the component of described substratum comprises that 20.1g/L 7.5% sodium chloride broth, 8.0g/L PEARLITOL 25C, 0.032g/L are phenol red, 0.5g/L potassium primary phosphate, 65.2g/L yolk emulsion, and pH value is 7.2-7.6.
Described 7.5% sodium chloride broth is containing Tryptones 10.0g, beef powder 5.0g, sodium-chlor 75.0g, dipotassium hydrogen phosphate 2.0g in often liter of 7.5% sodium chloride broth.
Peptone and beef powder provide nitrogenous source, VITAMIN and somatomedin; D-N.F,USP MANNITOL is fermentable saccharide; Sodium-chlor maintains balanced osmotic pressure; Agar is the peptizer of substratum; Phenol red is pH indicator.
Take this product 112.5g, be dissolved in 1000ml distilled water, boil 1 minute, 121 DEG C of autoclavings 15 minutes, when being cooled to 45-50 DEG C, aseptic technique adds yolk emulsion 3, shakes up, impouring sterilized petri dishes.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | Well-grown | Periphery of bacterial colonies produces β zone of hemolysis |
| Colon bacillus | Well-grown | γ haemolysis |
| Beta hemolytic streptococcus | Well-grown | Periphery of bacterial colonies produces β zone of hemolysis |
| Streptococcus pneumoniae | Well-grown | Periphery of bacterial colonies produces α zone of hemolysis |
Claims (3)
1. a N.F,USP MANNITOL egg yolk medium, it is characterized in that the component of described substratum comprises that 20.1g/L 7.5% sodium chloride broth, 8.0g/L PEARLITOL 25C, 0.032g/L are phenol red, 0.5g/L potassium primary phosphate, 65.2g/L yolk emulsion, pH value is 7.2-7.6.
2. N.F,USP MANNITOL egg yolk medium according to claim 1, is characterized in that described 7.5% sodium chloride broth is for containing Tryptones 10.0g, beef powder 5.0g, sodium-chlor 75.0g, dipotassium hydrogen phosphate 2.0g in often liter of 7.5% sodium chloride broth.
3. an application for N.F,USP MANNITOL egg yolk medium according to claim 1, is characterized in that the selective separation for streptococcus aureus is cultivated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599781.6A CN104651256A (en) | 2013-11-25 | 2013-11-25 | Mannitol-yolk medium and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599781.6A CN104651256A (en) | 2013-11-25 | 2013-11-25 | Mannitol-yolk medium and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651256A true CN104651256A (en) | 2015-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310599781.6A Pending CN104651256A (en) | 2013-11-25 | 2013-11-25 | Mannitol-yolk medium and use thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293713A (en) * | 2014-09-30 | 2015-01-21 | 青岛康合伟业商贸有限公司 | Mannitol egg yolk medium and application thereof |
-
2013
- 2013-11-25 CN CN201310599781.6A patent/CN104651256A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293713A (en) * | 2014-09-30 | 2015-01-21 | 青岛康合伟业商贸有限公司 | Mannitol egg yolk medium and application thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |