CN104313116A - Columbia blood agar base culture medium - Google Patents
Columbia blood agar base culture medium Download PDFInfo
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- CN104313116A CN104313116A CN201410521486.3A CN201410521486A CN104313116A CN 104313116 A CN104313116 A CN 104313116A CN 201410521486 A CN201410521486 A CN 201410521486A CN 104313116 A CN104313116 A CN 104313116A
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- culture medium
- meat
- substratum
- blood agar
- casein
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Abstract
The invention relates to the field of microorganisms, and in particular relates to a columbia blood agar base culture medium. The culture medium comprises the following components: 32.1g/L of meat infusion broth, 10.0g/L of casein pancreatic digest, 4.0g/L of heart casein pancreatic digest, 1.2g/L of corn starch, 4.5g/L of meat stomach enzyme digest, 4.5g/L of yeast extract powder, 0.18g/L of sodium azide and 13g/L of agar, and the pH value of the culture medium is 7.1 to 7.5. Every liter of the meat infusion broth contains 3.0g of beef powder, 5.0g of sodium chloride, 12.0g of casein tryptone and 2.0g of dipotassium phosphate. The culture medium is simple in formula, convenient to prepare, low in cost and convenient to popularize and apply; the cell is fast in growth and low in aberration rate; the culture medium is convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of columbia blood agar base substratum.
Background technology
One in microorganism culturing (microculture) biological culture.The main ill poison of the microorganism cultivated, bacterium, actinomycetes, fungi etc.Microorganism culturing needs to use substratum, prepares specific substratum according to the different sorts of microorganism and life habit.The successful key of microorganism culturing is aseptic technique, if cultivate utensil and substratum can not thoroughly sterilizing, cultivation process in have living contaminants to be easy to failure.
Faecalis (Enterococcus) belongs to enterococcus spp, it is a part for human and animal's normal intestinal flora, usually find in the mixing mycelia causing abdominal cavity to be separated with pelvic infection, previously think that faecalis is the commensalism bacterium harmless to the mankind, but research in recent years confirms enterococcal virulence.In aerobic gram positive coccus, it is only second to staphylococcic important Nosocomial Infection Pathogens, and faecalis also can cause community infection.Faecalis not only can cause urinary tract infections, skin soft-tissue infection, also can cause life-threatening abdominal cavity infection, septicemia, endocarditis and meningitis etc.
Hemolytic streptococcus, aerobic or facultative anaerobe, nutritional requirement is higher, poor growth on ordinary culture medium, need supplement serum, blood, ascites, and most of bacterial strain needs the somatomedins such as riboflavin, vitamin B6, nicotinic acid.Optimum growth temperature is 37 DEG C, can grow at 20-42 DEG C, and optimal pH is 7.4-7.6.Easily long-chain is become, in cotton-shaped or granular precipitate growth at the bottom of pipe in serum broth.Blood agar is formed the tiny bacterium colony of canescence, translucent, smooth surface, neat in edge, diameter 0.5-0.75mm, and different strains haemolysis differs.
Enterococcus faecalis, according to up-to-date principle of classification, streptococcus faecium is enterococcus faecalis again.Application C polysaccharide antigen, according to Lan Shi (Lancefield) serological classification, can be divided into many groups by suis.Streptococcus faecium belongs to D group.D group streptococcus divides faecalis and non-faecalis two class.The former comprises streptococcus faecium (S.faecalis), streptococcus faecalis (S.faecium) and streptococcus durans (S.durans), and the latter has streptococcus bovis (S.bovis) and streptococcus equinus (S.equinus).
Its nutritional requirement is low, and ordinary nutrient agar also can grow.Can grow at 10 DEG C or 45 DEG C of pH9.6 or containing in 6.5%NaCl broth culture, and ability 65 DEG C 30 minutes.It can utilize arginine for the energy, fermentation sorbyl alcohol, nonfermented pectinose.Simple substratum grows and does not need folic acid.DNA-DNA results of hybridization shows, faecalis and streptococcus degree of homology low, therefore have scholar to advise separately founding enterococcus spp, person relevant to the mankind is enterococcus faecalis (E.faecalis) and faecium (E.faecium).
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of columbia blood agar base substratum.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of columbia blood agar base substratum, the component of described substratum comprises 32.1g/L meat extract meat soup, 10.0g/L pancreatic digest of casein, 4.0g/L heart trysinization thing, 1.2g/L W-Gum, 4.5g/L meat peptic digest, 4.5g/L yeast extract powder, 0.18g/L sodiumazide, 13.0g/L agar, and pH value is 7.1-7.5.
On the basis of above scheme, described meat extract meat soup is containing beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g in often liter of meat extract meat soup.
Pancreatic digest of casein, meat peptic digest, heart trysinization thing and yeast extract powder provide carbon nitrogen source, VITAMIN and amino acid; Starch can promote the growth of Neisseria and strengthen streptococcic haematolysis property; Sodium-chlor maintains balanced osmotic pressure; Agar is the peptizer of substratum.
Take this product 39 grams, heating for dissolving is in 1000ml distilled water, and packing, 121 DEG C of autoclavings 15 minutes, when being chilled to about 45-50 DEG C, add the sterile sulfuric acid gentamicin being equivalent to 20mg gentamicin, mixing, impouring sterilized petri dishes.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of columbia blood agar base substratum, the component of described substratum comprises 32.1g/L meat extract meat soup, 10.0g/L pancreatic digest of casein, 4.0g/L heart trysinization thing, 1.2g/L W-Gum, 4.5g/L meat peptic digest, 4.5g/L yeast extract powder, 0.18g/L sodiumazide, 13.0g/L agar, and pH value is 7.1-7.5.
Described meat extract meat soup is containing beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g in often liter of meat extract meat soup.
Pancreatic digest of casein, meat peptic digest, heart trysinization thing and yeast extract powder provide carbon nitrogen source, VITAMIN and amino acid; Starch can promote the growth of Neisseria and strengthen streptococcic haematolysis property; Sodium-chlor maintains balanced osmotic pressure; Agar is the peptizer of substratum.
Take this product 39 grams, heating for dissolving is in 1000ml distilled water, and packing, 121 DEG C of autoclavings 15 minutes, when being chilled to about 45-50 DEG C, add the sterile sulfuric acid gentamicin being equivalent to 20mg gentamicin, mixing, impouring sterilized petri dishes.
18-24 hour is cultivated at 30-35 DEG C.
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | Well | There is xanthein |
| Singly increase listeria spp | Well | Water white transparency small colonies |
| Colon bacillus | Well | Water white transparency macrocolony |
| Salmonellas | Well | Colourless macrocolony |
| Pseudomonas aeruginosa | Well | There is green pigment |
Claims (2)
1. a columbia blood agar base substratum, it is characterized in that the component of described substratum comprises 32.1g/L meat extract meat soup, 10.0g/L pancreatic digest of casein, 4.0g/L heart trysinization thing, 1.2g/L W-Gum, 4.5g/L meat peptic digest, 4.5g/L yeast extract powder, 0.18g/L sodiumazide, 13.0g/L agar, pH value is 7.1-7.5.
2. according to claim a kind of columbia blood agar base substratum, is characterized in that described meat extract meat soup is for containing beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g in often liter of meat extract meat soup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410521486.3A CN104313116A (en) | 2014-09-30 | 2014-09-30 | Columbia blood agar base culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410521486.3A CN104313116A (en) | 2014-09-30 | 2014-09-30 | Columbia blood agar base culture medium |
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| CN104313116A true CN104313116A (en) | 2015-01-28 |
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| CN201410521486.3A Pending CN104313116A (en) | 2014-09-30 | 2014-09-30 | Columbia blood agar base culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3315597A4 (en) * | 2015-06-23 | 2018-11-21 | Viktor Veniaminovich Tets | Nutrient medium for cultivating bacteria |
| CN115997906A (en) * | 2022-12-28 | 2023-04-25 | 中国肉类食品综合研究中心 | Fermentation liquor, flavor modifier and application thereof in processing of low-salt preserved products |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816831A (en) * | 2012-08-01 | 2012-12-12 | 上海市疾病预防控制中心 | Kit for separating and identifying campylobacter rectus as well as preparation and application for kit |
| CN104651453A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Columbia blood agar-based medium |
-
2014
- 2014-09-30 CN CN201410521486.3A patent/CN104313116A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816831A (en) * | 2012-08-01 | 2012-12-12 | 上海市疾病预防控制中心 | Kit for separating and identifying campylobacter rectus as well as preparation and application for kit |
| CN104651453A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Columbia blood agar-based medium |
Non-Patent Citations (1)
| Title |
|---|
| NEOGEN: "Columbia blood agar base(7125)", 《COLUMBIA BLOOD AGAR BASE》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3315597A4 (en) * | 2015-06-23 | 2018-11-21 | Viktor Veniaminovich Tets | Nutrient medium for cultivating bacteria |
| US10745662B2 (en) | 2015-06-23 | 2020-08-18 | Viktor Veniaminovich Tets | Nutrient medium for cultivating bacteria |
| CN115997906A (en) * | 2022-12-28 | 2023-04-25 | 中国肉类食品综合研究中心 | Fermentation liquor, flavor modifier and application thereof in processing of low-salt preserved products |
| CN115997906B (en) * | 2022-12-28 | 2024-02-02 | 中国肉类食品综合研究中心 | Fermentation liquor, flavor modifier and application thereof in processing of low-salt preserved products |
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Application publication date: 20150128 |