CN104651469A - Bile liquid medium and use thereof - Google Patents
Bile liquid medium and use thereof Download PDFInfo
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- CN104651469A CN104651469A CN201310599450.2A CN201310599450A CN104651469A CN 104651469 A CN104651469 A CN 104651469A CN 201310599450 A CN201310599450 A CN 201310599450A CN 104651469 A CN104651469 A CN 104651469A
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- liquid medium
- bile
- bile liquid
- brain
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Abstract
The invention relates to the field of microbes and especially relates to a bile liquid medium. The bile liquid medium comprises 33g/L of brain-heart leachate broth, 22.0g/L of pig gall powder, and 0.88g/L of ferric citrate. The bile liquid medium has a pH value of 7.3-7.5. Each liter of the brain-heart leachate broth contains 12.0g of peptone, 12.5g of dehydrated calf brain extract powder, 5.0g of sodium chloride, 5.0g of glucose and 2.5g of disodium hydrogen phosphate. The bile liquid medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of bile liquid culture medium and application thereof.
background technology
One in microorganism culturing (microculture) biological culture.The main ill poison of the microorganism cultivated, bacterium, actinomycetes, fungi etc.Microorganism culturing needs to use substratum, prepares specific substratum according to the different sorts of microorganism and life habit.The successful key of microorganism culturing is aseptic technique, if cultivate utensil and substratum can not thoroughly sterilizing, cultivation process in have living contaminants to be easy to failure.
Faecalis (Enterococcus) belongs to enterococcus spp, it is a part for human and animal's normal intestinal flora, usually find in the mixing mycelia causing abdominal cavity to be separated with pelvic infection, previously think that faecalis is the commensalism bacterium harmless to the mankind, but research in recent years confirms enterococcal virulence.In aerobic gram positive coccus, it is only second to staphylococcic important Nosocomial Infection Pathogens, and faecalis also can cause community infection.Faecalis not only can cause urinary tract infections, skin soft-tissue infection, also can cause life-threatening abdominal cavity infection, septicemia, endocarditis and meningitis etc.
Hemolytic streptococcus, aerobic or facultative anaerobe, nutritional requirement is higher, poor growth on ordinary culture medium, need supplement serum, blood, ascites, and most of bacterial strain needs the somatomedins such as riboflavin, vitamin B6, nicotinic acid.Optimum growth temperature is 37 DEG C, can grow at 20-42 DEG C, and optimal pH is 7.4-7.6.Easily long-chain is become, in cotton-shaped or granular precipitate growth at the bottom of pipe in serum broth.Blood agar is formed the tiny bacterium colony of canescence, translucent, smooth surface, neat in edge, diameter 0.5-0.75mm, and different strains haemolysis differs.
Enterococcus faecalis, according to up-to-date principle of classification, streptococcus faecium is enterococcus faecalis again.Application C polysaccharide antigen, according to Lan Shi (Lancefield) serological classification, can be divided into many groups by suis.Streptococcus faecium belongs to D group.D group streptococcus divides faecalis and non-faecalis two class.The former comprises streptococcus faecium (S.faecalis), streptococcus faecalis (S.faecium) and streptococcus durans (S.durans), and the latter has streptococcus bovis (S.bovis) and streptococcus equinus (S.equinus).
Its nutritional requirement is low, and ordinary nutrient agar also can grow.Can grow at 10 DEG C or 45 DEG C of pH 9.6 or containing in 6.5%NaCl broth culture, and ability 65 DEG C 30 minutes.It can utilize arginine for the energy, fermentation sorbyl alcohol, nonfermented pectinose.Simple substratum grows and does not need folic acid.DNA-DNA results of hybridization shows, faecalis and streptococcus degree of homology low, therefore have scholar to advise separately founding enterococcus spp, person relevant to the mankind is enterococcus faecalis (E.faecalis) and faecium (E.faecium).
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of bile liquid culture medium and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of bile liquid culture medium, the component of described substratum comprises 33g/L brain heart infusion broth, 22.0g/L Pulvis Fellis Suis, 0.88g/L ironic citrate, and pH value is 7.3-7.5; Described brain heart infusion broth is containing peptone 12.0g, dehydration calf brain leaching powder 12.5g, sodium-chlor 5.0g, glucose 5.0g, Sodium phosphate dibasic 2.5g in often liter of meat soup.
The application that the invention also discloses bile liquid culture medium is tested for the bile tolerant of streptococcus faecium.
Peptone, dehydration calf brain leaching powder, dehydration beef heart infusion provide nitrogenous source, VITAMIN and somatomedin; Glucose can be various bacteria and provides the energy; Sodium-chlor maintains balanced osmotic pressure; Sodium phosphate dibasic is buffer reagent.
Take this product 45g, heating for dissolving is in 1000ml distilled water, and 121 DEG C of autoclavings 15 minutes, are chilled to 45 ± 1 DEG C, and impouring is containing about 15ml substratum in 1ml sample liquid plate, and mixing, solidifies.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of bile liquid culture medium, the component of described substratum comprises 33g/L brain heart infusion broth, 22.0g/L Pulvis Fellis Suis, 0.88g/L ironic citrate, and pH value is 7.3-7.5; Described brain heart infusion broth is containing peptone 12.0g, dehydration calf brain leaching powder 12.5g, sodium-chlor 5.0g, glucose 5.0g, Sodium phosphate dibasic 2.5g in often liter of meat soup.
Peptone, dehydration calf brain leaching powder, dehydration beef heart infusion provide nitrogenous source, VITAMIN and somatomedin; Glucose can be various bacteria and provides the energy; Sodium-chlor maintains balanced osmotic pressure; Sodium phosphate dibasic is buffer reagent.
Take this product 45g, heating for dissolving is in 1000ml distilled water, and 121 DEG C of autoclavings 15 minutes, are chilled to 45 ± 1 DEG C, and impouring is containing about 15ml substratum in 1ml sample liquid plate, and mixing, solidifies.
Cultivate 24h for 35 ± 0.5 DEG C, result is as follows:
| Bacterium name | Upgrowth situation | Cultural characteristic |
| Excrement suis | Well | Meat soup is muddy |
| Beta hemolytic streptococcus | Well | Meat soup is muddy |
| Colon bacillus | Well | Meat soup is muddy |
Claims (2)
1. a bile liquid culture medium, it is characterized in that the component of described substratum comprises 33g/L brain heart infusion broth, 22.0g/L Pulvis Fellis Suis, 0.88g/L ironic citrate, pH value is 7.3-7.5; Described brain heart infusion broth is containing peptone 12.0g, dehydration calf brain leaching powder 12.5g, sodium-chlor 5.0g, glucose 5.0g, Sodium phosphate dibasic 2.5g in often liter of meat soup.
2. an application for bile liquid culture medium according to claim 1, is characterized in that the bile tolerant for streptococcus faecium is tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599450.2A CN104651469A (en) | 2013-11-25 | 2013-11-25 | Bile liquid medium and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599450.2A CN104651469A (en) | 2013-11-25 | 2013-11-25 | Bile liquid medium and use thereof |
Publications (1)
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| CN104651469A true CN104651469A (en) | 2015-05-27 |
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| CN201310599450.2A Pending CN104651469A (en) | 2013-11-25 | 2013-11-25 | Bile liquid medium and use thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862370A (en) * | 2015-06-19 | 2015-08-26 | 钟伟萍 | Bacteroides fragilis selective culture medium |
-
2013
- 2013-11-25 CN CN201310599450.2A patent/CN104651469A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862370A (en) * | 2015-06-19 | 2015-08-26 | 钟伟萍 | Bacteroides fragilis selective culture medium |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |