CN104195215A - Enterococcus agar medium and application thereof - Google Patents
Enterococcus agar medium and application thereof Download PDFInfo
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- CN104195215A CN104195215A CN201410459280.2A CN201410459280A CN104195215A CN 104195215 A CN104195215 A CN 104195215A CN 201410459280 A CN201410459280 A CN 201410459280A CN 104195215 A CN104195215 A CN 104195215A
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Abstract
The invention belongs to the field of microorganisms and in particular relates to an enterococcus agar medium. The medium comprises the following components: meat infusion broth, 4.0g/L of yeast extract powder, 12g/L of ox bile, 0.88g/L of sodium citrate, 0.45g/L of ammonium ferric citrate, 2g/L of potassium dihydrogen phosphate, 0.17g/L of sodium azide, 0.9g/L of aesculin and 15.0g/L of agar, wherein the pH value of the medium is 6.9-7.3; every 1L of meat infusion broth contains 3.0g of beef powder, 5.0g of sodium chloride, 12.0g of casein tryptone and 2.0g of dipotassium hydrogen phosphate. The enterococcus agar medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of enterococcosel agar substratum and application thereof.
Background technology
A kind of in microorganism culturing (microculture) biological culture.The main ill poison of microorganism of cultivating, bacterium, actinomycetes, fungi etc.Microorganism culturing need to be used substratum, according to the different sorts of microorganism and life habit, prepares specific substratum.The successful key of microorganism culturing is aseptic technique, if having living contaminants to be easy to failure in the process of cultivation utensil and thoroughly sterilizing of substratum, cultivation.
Faecalis (Enterococcus) belongs to enterococcus spp, it is a part for human and animal's normal intestinal flora, conventionally in causing the abdominal cavity mixing mycelia separated with pelvic infection, find, previously think that faecalis is the commensalism bacterium harmless to the mankind, but research in recent years has confirmed enterococcal virulence.In aerobic gram positive coccus, it is to be only second to staphylococcic important Nosocomial Infection Pathogens, and faecalis also can cause community infection.Faecalis not only can cause urinary tract infections, skin soft-tissue infection, also can cause life-threatening abdominal cavity infection, septicemia, endocarditis and meningitis etc.
Hemolytic streptococcus, aerobic or facultative anaerobe, nutritional requirement is higher, and poor growth on ordinary culture medium needs to supplement serum, blood, ascites, and most of bacterial strains need the somatomedins such as riboflavin, vitamin B6, nicotinic acid.Optimum growth temperature is 37 ℃, at 20-42 ℃, can grow, and optimal pH is 7.4-7.6.In serum broth, easily become long-chain, the pipe end, is cotton-shaped or particulate state precipitation growth.The tiny bacterium colony that forms canescence, translucent, smooth surface, neat in edge, diameter 0.5-0.75mm on blood agar, different strains haemolysis differs.
Enterococcus faecalis, according to up-to-date principle of classification, streptococcus faecium is again enterococcus faecalis.Application C polysaccharide antigen, according to Lan Shi (Lancefield) serological classification, can be divided into suis many groups.Streptococcus faecium belongs to D group.D group streptococcus divides faecalis and non-faecalis two classes.The former comprises streptococcus faecium (S.faecalis), streptococcus faecalis (S.faecium) and streptococcus durans (S.durans), and the latter has streptococcus bovis (S.bovis) and streptococcus equinus (S.equinus).
Its nutritional requirement is low, on ordinary nutrient agar, also can grow.Can or grow containing in 6.5%NaCl broth culture at 10 ℃ or 45 ℃ of pH9.6, and 65 ℃ of abilities 30 minutes.It can utilize arginine for the energy, fermentation sorbyl alcohol, nonfermented pectinose.On simple substratum, growth does not need folic acid.The demonstration of DNA-DNA results of hybridization, faecalis and streptococcus homology degree are low, therefore there is scholar to advise separately standing enterococcus spp, person relevant to the mankind is enterococcus faecalis (E.faecalis) and faecium (E.faecium).
Substratum (Medium) is for the nutriment of microorganism, plant and animal tissue growth and the artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single kind microorganism culturing and evaluation.Substratum is because the raw material of preparation is different, and service requirements is different, and storage and custody aspect is also slightly different.General substratum is being heated, after the moisture absorption, is easily contaminated by bacterial or decomposes rottenly, and therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of strict sterilizing, and the storage of long period, must be placed in the refrigerator of 3～6 ℃.Because liquid nutrient medium is difficult for long-term keeping, all change system into now powder.
Substratum is natural medium, combination substratum according to chemical classification and partly combines substratum; According to physical classification, be liquid nutrient medium, film solid media and dehydrated medium; According to microorganism classification, be selective medium, differential medium.
Summary of the invention
The present invention overcomes deficiency of the prior art, and a kind of enterococcosel agar substratum and application thereof are provided.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, the row filter that is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factors such as the pH value of substratum, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding are compared, worked out substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of enterococcosel agar substratum, the component of described substratum comprises that meat extract meat soup, 4.0g/L yeast soak powder, 12g/L oxgall thing, 0.88g/L Trisodium Citrate, 0.45g/L ferric ammonium citrate, 2g/L potassium primary phosphate 0.17g/L sodiumazide, 0.9g/L Vitamin C2,15.0g/L agar, and pH value is 6.9-7.3.
On the basis of above scheme, described meat extract meat soup is in every liter of meat extract meat soup, to contain beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g.
The invention also discloses a kind of application of enterococcosel agar substratum, for the enterococcal counting of Food and water.
Pancreas casein peptone and beef extract powder, yeast powder provide nitrogenous source, VITAMIN and somatomedin; Sodium-chlor maintains balanced osmotic pressure; Bacterium hydrolysis Vitamin C2 reacts the Esculetin that forms black with iron ion in ferric ammonium citrate; Trisodium Citrate, bilein and sodiumazide are selectivity fungistat, suppress non-streptococcic bacterium; Agar is the peptizer of substratum.
Take this product 56.3g, heating for dissolving is in 1000ml distilled water, and 121 ℃ of autoclavings 15 minutes, are chilled to 45 ± 1 ℃, and impouring, containing about 15ml substratum in 1ml sample liquid plate, mixes, and solidifies.Be inverted culture medium culturing 24 ± 2h for 35 ± 2 ℃.
Note: start not surpass 4h to making the dull and stereotyped time from insulation.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of enterococcosel agar substratum, the component of described substratum comprises that meat extract meat soup, 4.0g/L yeast soak powder, 12g/L oxgall thing, 0.88g/L Trisodium Citrate, 0.45g/L ferric ammonium citrate, 2g/L potassium primary phosphate 0.17g/L sodiumazide, 0.9g/L Vitamin C2,15.0g/L agar, and pH value is 6.9-7.3.
Described meat extract meat soup is in every liter of meat extract meat soup, to contain beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g.
Take this product 56.3g, heating for dissolving is in 1000ml distilled water, and 121 ℃ of autoclavings 15 minutes, are chilled to 45 ± 1 ℃, and impouring, containing about 15ml substratum in 1ml sample liquid plate, mixes, and solidifies.Be inverted culture medium culturing 24 ± 2h for 35 ± 2 ℃.
Note: start not surpass 4h to making the dull and stereotyped time from insulation.
At 36 ± 0.5 ℃, cultivate 24 hours
| Bacterium name | Upgrowth situation | Cultural characteristic |
| Streptococcus faecium | Well | Dark brown city, has brown city halo |
| Golden yellow city staphylococcus | Suppressed | - |
| Colon bacillus | Suppressed | - |
Embodiment in above-mentioned embodiment is illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore do not departing from any variation and the modification of making under general plotting of the present invention, within all should belonging to the protection domain that the present invention limits.
Claims (3)
1. an enterococcosel agar substratum, the component that it is characterized in that described substratum comprises that meat extract meat soup, 4.0g/L yeast soak powder, 12g/L oxgall thing, 0.88g/L Trisodium Citrate, 0.45g/L ferric ammonium citrate, 2g/L potassium primary phosphate 0.17g/L sodiumazide, 0.9g/L Vitamin C2,15.0g/L agar, and pH value is 6.9-7.3.
2. according to claim a kind of enterococcosel agar substratum, is characterized in that described meat extract meat soup is in every liter of meat extract meat soup, to contain beef powder 3.0g, sodium-chlor 5.0g, pancreas casein peptone 12.0g, dipotassium hydrogen phosphate 2.0g.
3. an application for according to claim a kind of enterococcosel agar substratum, is characterized in that for the enterococcal counting of Food and water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410459280.2A CN104195215A (en) | 2014-09-09 | 2014-09-09 | Enterococcus agar medium and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410459280.2A CN104195215A (en) | 2014-09-09 | 2014-09-09 | Enterococcus agar medium and application thereof |
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| CN104195215A true CN104195215A (en) | 2014-12-10 |
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| CN201410459280.2A Pending CN104195215A (en) | 2014-09-09 | 2014-09-09 | Enterococcus agar medium and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733974A (en) * | 2014-12-11 | 2016-07-06 | 明儒成 | Selective enterococcus enrichment medium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| CN104651461A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Enterococcus agar culture medium and use thereof |
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2014
- 2014-09-09 CN CN201410459280.2A patent/CN104195215A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| CN104651461A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Enterococcus agar culture medium and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 周英等: "粪肠球菌F71的增殖培养基优化研究", 《现代农业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733974A (en) * | 2014-12-11 | 2016-07-06 | 明儒成 | Selective enterococcus enrichment medium |
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Application publication date: 20141210 |