CN104642399A - Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot - Google Patents
Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot Download PDFInfo
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- CN104642399A CN104642399A CN201510035884.9A CN201510035884A CN104642399A CN 104642399 A CN104642399 A CN 104642399A CN 201510035884 A CN201510035884 A CN 201510035884A CN 104642399 A CN104642399 A CN 104642399A
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Abstract
The invention discloses application of an artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot, belonging to the technical fields of plant pest prevention and control. A preparation method of the artemisia verlotorum extract comprises the following steps of (1) naturally airing after cleaning fresh stems and leaves of disease-free artemisia verlotorum, sieving after smashing the fresh stems and leaves of the disease-free artemisia verlotorum, then, mixing the stem powder and the leaf powder, and placing the mixed powder in a dry vessel; (2) adding ethanol into the artemisia verlotorum powder obtained in the step (1) to filter for the first time to obtain a filtrate, adding ethanol into the obtained filter residue again to filter for the second time, repeatedly filtering twice, and then, mixing the filtrates obtained in three times; and (3) carrying out reduced-pressure condensation and ultraviolet sterilization on the filtrate obtained in the step (2), and then, keeping the constant volume by using sterile water to obtain the artemisia verlotorum extract. The artemisia verlotorum extract disclosed by the invention has a favorable antibacterial effect for pathogenic bacteria of panax pseudoginseng root rot.
Description
Technical field
The present invention relates to techniques of pest control field, particularly relate to the purposes of southern biennial wormwood extract control notoginseng root rot.
Background technology
South argy wormwood (Artemisia verlotorum Lamotte), belongs to composite family artemisia, another name Artemisia santonica, Artemisia stelleriana.Because sagebruss is similar on profile, smell, also can be collectively referred to as " wormwood " or " argy wormwood ", large portion of China, there is distribution Europe, Asia and Africa etc.Adopt this plant for perennial, plant has fragrance.Main root is slightly obvious, and side root is many.The life of stem list or minority, high 50-100 centimetre, the long 5-8 centimetre of branch, is affixed to stem obliquely.Be seen everywhere in the wild, occupy an important position in traditional Chinese culture.Can be used as medicine, have anti-inflammatory, anastalsis.
Pseudo-ginseng (Panax notoginseng F.H.chen) has another name called pseudo-ginseng, invaluable etc., and having promoting blood circulation and removing blood stasis, detumescence ding-tong effect significantly, is a kind of Chinese tradition rare medicinal herbs.Be that the Chinese patent drug that primary raw material is made is as wide-spread in " Yunnan Baiyao " and " Pien Tze Huang " etc. with pseudo-ginseng.In recent years, growing to the raw-material demand of pseudo-ginseng.But the disease in cultivation has had a strong impact on Panax notoginseng Growth, and the abuse of chemical pesticide also have impact on the quality of pseudo-ginseng.And notoginseng root rot is one of pseudo-ginseng Major Diseases, be commonly called as " green smelly " in producing region, cardinal symptom is that the bud-rot in seedling stage and other reed head and the brown water stain shape pathology of bastem junction are until spread to whole cane base portion.Just there is generation in seeding stage, 4, May disease stagnate, the 6-9 month enters rainy season, enters onset peak period.Root rot has polytype, it is reported its symptom mostly caused by notoginseng root rot pathogen (Fusarium scirpi lamb) endangers.Notoginseng root rot is the main cause causing pseudo-ginseng product quality in recent years to decline.
Southern biennial wormwood extract of the present invention has good fungistatic effect to the former bacterium of notoginseng root rot (Fusarium scirpi lamb), and this bacteriostasis has positive using value, and the biological prevention for pseudo-ginseng disease is significant.
Summary of the invention
The object of the invention is the purposes providing a kind of southern biennial wormwood extract control notoginseng root rot.The technical scheme that the present invention takes is:
The invention provides the purposes of southern biennial wormwood extract control notoginseng root rot.
In addition, present invention also offers the preparation method of southern biennial wormwood extract, its concrete steps are as follows:
(1) choose disease-free southern argy wormwood stem, Ye Xianti dries after cleaning naturally, fresh body grinding and sieving, then stem, the mixing of leaf powder are placed in drying receptacle;
(2) in the argy wormwood powder of step (1) gained south, add ethanol carry out first time and filter, obtain filtrate, gained filter residue is added ethanol again and carry out second time filtration, after repeating filtration 2 times, merge 3 filtrates;
(3) by step (2) gained filtrate through reduced pressure concentration, use ultraviolet sterilization, then namely obtain southern biennial wormwood extract of the present invention with sterile water constant volume.
In technical solution of the present invention, the aperture adopting sieve of sieving described in step (1) is 50 orders.
In technical solution of the present invention, first time described in step (2) filters, and is in southern argy wormwood powder, add 8 times amount ethanol soak 4d under room temperature, then uses four layers of filtered through gauze, obtain filtrate.
In technical solution of the present invention, described in step (2), second time is filtered, and is to be filtered gained filter residue 6 times amount ethanol first time to soak 4d under room temperature, then uses four layers of filtered through gauze, obtains filtrate.
In technical solution of the present invention, the concentration of step (3) Central-South biennial wormwood extract is 2.0gmL
-1.
Compared with prior art, the beneficial effect that the present invention has is:
Southern biennial wormwood extract of the present invention obviously can suppress the former bacterium of notoginseng root rot, and its inhibitory action declines along with the prolongation of crude drug concentration, incubation time, and therefore, southern biennial wormwood extract has the prospect that exploitation becomes a kind of botanical fungicide.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.
embodiment 1: the preparation of southern biennial wormwood extract
Choose disease-free southern argy wormwood stem, Ye Xianti dries after cleaning naturally.It is 50 object sieves that fresh body pulverized aperture, then its stem, the mixing of leaf powder is placed in drying receptacle, adds 8 times amount ethanol and soak 4d under room temperature, then use four layers of filtered through gauze, obtain filtrate.Filter residue 6 times amount ethanol repeat previous step dip operation, after repeating 2 times, merge three filtrates.Filtrate decompression, concentrated, after using ultraviolet sterilization, being then settled to crude drug concentration with sterile water is 2.0gmL
-1.
embodiment 2: the bacteriostasis of southern biennial wormwood extract
2.1 materials and reagent
South argy wormwood ethanol extract, sterile water, PDA medium.For examination bacterial classification: the former bacterium of notoginseng root rot (Fusarium scirpi lamb) is provided by agricultural college of Guangxi University plant pathology laboratory.
2.2 method
Mycelial growth rate method is adopted to measure the fungistatic effect of southern biennial wormwood extract.Measuring crude drug concentration is 2.0gmL
-1southern argy wormwood leaching liquor 6.75ml, 3.375ml, 1.6875ml, 10ml is settled to respectively with sterile water, the southern argy wormwood test solution of obtained variable concentrations, get the southern argy wormwood test liquid 1ml of variable concentrations respectively, add the PDA medium 14ml melted and make pastille flat board in 90mm culture dish, make it finally supply examination concentration to be 0.0900gmL
-1, 0.0450gmL
-1and 0.0225gmL
-1, replace extract for contrast to add equivalent sterile water.After culture medium solidifying, in the pathogen species cultivated in advance, beat cut-off footpath 6mm mycelia block with card punch, be transferred to the central authorities of culture dish, every culture dish inoculates one piece, cultivates 96h for 28 DEG C, each for trying concentration in triplicate.The 48th, 72h, 96h adopt right-angled intersection method to measure colony diameter, gets the average result repeated for three times, obtain variable concentrations extract to the inhibition for thing bacterium.
Following formulae discovery is to the inhibiting rate of pathogen:
Bacteriostasis rate (%)=[(contrast bacterium colony pure amount of growth-pure amount of growth of process the bacterium colony)/pure amount of growth of contrast bacterium colony] × 100%; Pure amount of growth=bacterium colony average diameter-bacterium cake diameter
The southern biennial wormwood extract of table 1 variable concentrations is to the inhibitory action of notoginseng root rot pathogen
As shown in Table 1, be 0.0900gmL in southern biennial wormwood extract crude drug concentration
-1, inhibiting rate is up to 74.42% when cultivating 48h, cultivate after 96h and can also reach 68.09% more than 50%, visible, southern biennial wormwood extract obviously can suppress the growth of the former bacterium of notoginseng root rot (Fusarium scirpi lamb); And low concentration south biennial wormwood extract reduces with the prolongation of incubation time the former bacterium inhibiting rate of notoginseng root rot.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (6)
1. the purposes of southern biennial wormwood extract control notoginseng root rot.
2. a preparation method for southern biennial wormwood extract as claimed in claim 1, is characterized in that: described method concrete steps are as follows:
(1) choose disease-free southern argy wormwood stem, Ye Xianti dries after cleaning naturally, fresh body grinding and sieving, then stem, the mixing of leaf powder are placed in drying receptacle;
(2) in the argy wormwood powder of step (1) gained south, add ethanol carry out first time and filter, obtain filtrate, gained filter residue is added ethanol again and carry out second time filtration, after repeating filtration 2 times, merge 3 filtrates;
(3) by step (2) gained filtrate through reduced pressure concentration, use ultraviolet sterilization, then namely obtain southern biennial wormwood extract of the present invention with sterile water constant volume.
3. preparation method as claimed in claim 2, is characterized in that: the aperture adopting sieve of sieving described in step (1) is 50 orders.
4. preparation method as claimed in claim 2, is characterized in that: first time described in step (2) filters, and is in southern argy wormwood powder, add 8 times amount ethanol soak 4d under room temperature, then uses four layers of filtered through gauze, obtain filtrate.
5. preparation method as claimed in claim 2, is characterized in that: described in step (2), second time is filtered, and is to be filtered gained filter residue 6 times amount ethanol first time to soak 4d under room temperature, then uses four layers of filtered through gauze, obtains filtrate.
6. preparation method as claimed in claim 2, is characterized in that: the concentration of step (3) Central-South biennial wormwood extract is 2.0gmL
-1.
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| CN201510035884.9A CN104642399B (en) | 2015-01-23 | 2015-01-23 | The purposes of southern biennial wormwood extract preventing and treating notoginseng root rot |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409654A (en) * | 2015-11-04 | 2016-03-23 | 云南中医学院 | Method for inhibiting panax notoginseng from root rot by cover matrix |
| CN105494334A (en) * | 2015-12-08 | 2016-04-20 | 北京农学院 | Environmental-friendly sterilizing agent capable of preventing and treating bacterial diseases of fruit trees and preparation method of environment-friendly sterilizing agent |
| CN105594771A (en) * | 2016-02-22 | 2016-05-25 | 云南笃行生物科技有限公司 | Pseudo-ginseng root rot prevention and control preparation |
| CN109463181A (en) * | 2018-09-12 | 2019-03-15 | 通化师范学院 | The application of purslane and argy wormwood in prevention and treatment ginseng root rot |
| CN109874811A (en) * | 2019-03-08 | 2019-06-14 | 湖北中医药大学 | A kind of natural inhibitor and preparation method thereof effectively preventing chrysanthemum root rot |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409654A (en) * | 2015-11-04 | 2016-03-23 | 云南中医学院 | Method for inhibiting panax notoginseng from root rot by cover matrix |
| CN105494334A (en) * | 2015-12-08 | 2016-04-20 | 北京农学院 | Environmental-friendly sterilizing agent capable of preventing and treating bacterial diseases of fruit trees and preparation method of environment-friendly sterilizing agent |
| CN105494334B (en) * | 2015-12-08 | 2018-03-13 | 北京农学院 | A kind of environmentally friendly bactericide for preventing and treating fruit tree bacterial disease and preparation method thereof |
| CN105594771A (en) * | 2016-02-22 | 2016-05-25 | 云南笃行生物科技有限公司 | Pseudo-ginseng root rot prevention and control preparation |
| CN109463181A (en) * | 2018-09-12 | 2019-03-15 | 通化师范学院 | The application of purslane and argy wormwood in prevention and treatment ginseng root rot |
| CN109874811A (en) * | 2019-03-08 | 2019-06-14 | 湖北中医药大学 | A kind of natural inhibitor and preparation method thereof effectively preventing chrysanthemum root rot |
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