CN109463181A - The application of purslane and argy wormwood in prevention and treatment ginseng root rot - Google Patents
The application of purslane and argy wormwood in prevention and treatment ginseng root rot Download PDFInfo
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- CN109463181A CN109463181A CN201811061156.5A CN201811061156A CN109463181A CN 109463181 A CN109463181 A CN 109463181A CN 201811061156 A CN201811061156 A CN 201811061156A CN 109463181 A CN109463181 A CN 109463181A
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- purslane
- extract
- root rot
- ginseng root
- prevention
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Landscapes
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the application of purslane and argy wormwood in prevention and treatment ginseng root rot.Have the beneficial effect that the purslane extract announced and biennial wormwood extract of the invention have high Collaboration effect, there is significant inhibitory effect to ginseng root rot opportunistic pathogen, increase the quality and yield of ginseng, and two kinds of extracts are easy degradation in a natural environment, it is not easy to remain, there is good economic and environment-friendly benefit.
Description
Technical field
The present invention relates to plant protection art, the answering in prevention and treatment ginseng root rot more particularly, to purslane and argy wormwood
With.
Background technique
Ginseng root rot mainly infects root and the blade of ginseng, causes root softening to rot, the flavescence of blade chlorisis, here withers
It is dead.Ginseng root rot causes ginseng to drop in production over a large area, quality sharp fall, causes huge economic loss, at present control
The main method of ginseng root rot processed is still based on chemical bactericide, but since chemical bactericide is easy to appear drug resistance and drug
Residual, not only pollutes environment, also seriously threatens human health.Since the 1980s, the numerous scientists in the world
Just the public nuisance-free agricultural chemicals of searching is efficient, low toxicity, low-residual and environment harmony are as the developing direction of pesticide research and exploitation.
Summary of the invention
The object of the present invention is to provide the application of purslane and argy wormwood in prevention and treatment ginseng root rot, make the anti-root rot of ginseng
Ability be obviously improved, increase the quality and yield of ginseng, and medicament residue and environmental pollution will not occur, have it is good
Economic and environment-friendly benefit.
Specifically, the present invention, which is provided, is used to prevent and treat ginseng root-rot for the composition of purslane extract and biennial wormwood extract
Purposes in disease.Purslane extract and biennial wormwood extract have high Collaboration effect, have to ginseng root rot opportunistic pathogen significant
Inhibitory effect, improve the ability of the anti-root rot of ginseng, increase the quality and yield of ginseng, and two kinds of extracts are in nature
It is easy degradation under environment, is not easy to remain, there is good economic and environment-friendly benefit.
Preferably, the preparation of composition comprises the steps of:
Purslane plants material and artemisia argyi plant material are extracted, separately in a solvent to obtain extracting solution；
Every kind of extracting solution is separately concentrated to provide purslane extract and biennial wormwood extract；And
Purslane extract and biennial wormwood extract are mixed, composition is formed.Under this condition, purslane and artemisia argyi plant material
The active constituent recovery rate of material is high, and purity is big, and the destruction that active constituent is subject to is small, obtains purslane and artemisia argyi plant material resources
To making full use of.
Preferably, solvent is selected from the ethyl alcohol that distilled water, ethyl acetate and mass fraction are 95%.Selected solvent source is rich
Richness, price is low, and can recycle, and reduces costs.
Preferably, the solid-liquid ratio of solvent and vegetable material is 1g:16-20mL.The solid-liquid ratio provides appropriate concentration
Gradient provides sufficient power for the substance transmitting in extraction process, and solvent consumption is less under this condition, has saved money
Source has good environmental protection benefit.
Preferably, it is extracted as ultrasonic extraction, extraction time 10-12h.Purslane and artemisia argyi plant material internal or more
Or some microbubbles have been dissolved less, these bubbles generate vibration, when acoustic pressure reaches certain value, gas under the action of ultrasonic wave
Bubble increases due to orientation diffusion, forms resonant cavity, then unexpected closure, can generate thousands of a atmosphere around it in closure
The pressure of pressure, cell wall rupture can be caused by being formed by micro laser wave, and entire rupture process is completed in moment, is conducive to purslane
With the dissolution of argy wormwood bacteriostatic active ingredients.
Preferably, Purslane plants material is the aerial part of Purslane plants, artemisia argyi plant material is artemisia argyi plant
Aerial part.
Preferably, thickening temperature is 35-40 DEG C, concentration time 20-25min.Under this condition, providing in extracting solution
The concentration of antipathogenic composition enhances its fungistatic effect, and the resulting solvent of concentrated extracting solution can be used to work as next group plant material
The solvent extracted is expected, to reduce solvent usage.
Preferably, the mass ratio of purslane extract and biennial wormwood extract is 1:5.Under this condition, purslane extract
Its synergistic effect can be farthest played with biennial wormwood extract, improves composition to the inhibiting effect of ginseng root rot opportunistic pathogen.
Compared with prior art, the invention has the benefit that there is height to assist for purslane extract and biennial wormwood extract
Same-action has significant inhibitory effect to ginseng root rot opportunistic pathogen, increases the quality and yield of ginseng, and two kinds of extracts
It is easy degradation in a natural environment, is not easy to remain, there is good economic and environment-friendly benefit.
Specific embodiment
It is described in further detail by the following examples:
Embodiment 1: the preparation of the composition comprising purslane extract and biennial wormwood extract, comprising the following steps:
1) it pre-processes: choosing disease-free Purslane plants material and artemisia argyi plant material, which is horse
The aerial part of bitterroot plant, artemisia argyi plant material are the aerial parts of artemisia argyi plant, and Purslane plants material and argy wormwood are planted
Object material is rinsed well, and naturally dry is crushed with medicinal herb grinder respectively, is crossed 50 meshes, is obtained purslane powder and argy wormwood powder；
2) extract: being separately added into mass fraction into purslane powder and argy wormwood powder by solid-liquid ratio 1g:19mL is 95%
Ethyl alcohol, ultrasonic extraction 10h, filtering gained extracting solution, 20min is concentrated at 35 DEG C in gained filtrate, respectively obtains purslane
Extract and biennial wormwood extract；
3) mix: 1:5 mixing purslane extract and biennial wormwood extract in mass ratio form composition.
Embodiment 2: the preparation of the composition comprising purslane extract and biennial wormwood extract, comprising the following steps:
1) it pre-processes: choosing disease-free Purslane plants material and artemisia argyi plant material, which is horse
The aerial part of bitterroot plant, artemisia argyi plant material are the aerial parts of artemisia argyi plant, and Purslane plants material and argy wormwood are planted
Object material is rinsed well, and naturally dry is crushed with medicinal herb grinder respectively, is crossed 50 meshes, is obtained purslane powder and argy wormwood powder；
2) extract: being separately added into mass fraction into purslane powder and argy wormwood powder by solid-liquid ratio 1g:18mL is 95%
Ethyl alcohol, ultrasonic extraction 12h, filtering gained extracting solution, 25min is concentrated at 40 DEG C in gained filtrate, respectively obtains purslane
Extract and biennial wormwood extract；
3) mix: 1:5 mixing purslane extract and biennial wormwood extract in mass ratio form composition.
Embodiment 3: the preparation of the composition comprising purslane extract and biennial wormwood extract, comprising the following steps:
1) it pre-processes: choosing disease-free Purslane plants material and artemisia argyi plant material, which is horse
The aerial part of bitterroot plant, artemisia argyi plant material are the aerial parts of artemisia argyi plant, and Purslane plants material and argy wormwood are planted
Object material is rinsed well, and naturally dry is crushed with medicinal herb grinder respectively, is crossed 50 meshes, is obtained purslane powder and argy wormwood powder；
2) it extracts: being separately added into distilled water, ultrasonic extraction into purslane powder and argy wormwood powder by solid-liquid ratio 1g:17mL
11h, filtering gained extracting solution, is concentrated 25min at 35 DEG C for gained filtrate, respectively obtains purslane extract and argy wormwood is extracted
Object；
3) mix: 1:5 mixing purslane extract and biennial wormwood extract in mass ratio form composition.
Embodiment 4: the preparation of the composition comprising purslane extract and biennial wormwood extract, comprising the following steps:
1) it pre-processes: choosing disease-free Purslane plants material and artemisia argyi plant material, which is horse
The aerial part of bitterroot plant, artemisia argyi plant material are the aerial parts of artemisia argyi plant, and Purslane plants material and argy wormwood are planted
Object material is rinsed well, and naturally dry is crushed with medicinal herb grinder respectively, is crossed 50 meshes, is obtained purslane powder and argy wormwood powder；
2) extract: being separately added into mass fraction into purslane powder and argy wormwood powder by solid-liquid ratio 1g:16mL is 95%
Ethyl alcohol, ultrasonic extraction 10h, filtering gained extracting solution, 23min is concentrated at 36 DEG C in gained filtrate, respectively obtains purslane
Extract and biennial wormwood extract；
3) mix: 1:5 mixing purslane extract and biennial wormwood extract in mass ratio add and account for biennial wormwood extract quality
0.23 ‰ 3,5- resacetophenone and the 3- methyl benzophenone for accounting for biennial wormwood extract quality 0.23 ‰ form composition,
The 3,5- resacetophenone and 3- methyl benzophenone being added improve purslane extract and biennial wormwood extract to cause of disease
The affinity of lanosterol C-14 α demethylase, greatly inhibits the activity of the enzyme contained by bacterium, to interrupt wheat
The biosynthesis of burnt sterol causes the ingredient of cytoplasma membrane to change, and reduces the stability of cytoplasma membrane structure, enhances
The permeability of cytoplasma membrane results in the extravasation of a large amount of nucleic acid and protein, affects the metabolism of cell, has reached antibacterial
Effect.
Embodiment 5: the preparation of the composition comprising purslane extract and biennial wormwood extract, comprising the following steps:
1) it pre-processes: choosing disease-free Purslane plants material and artemisia argyi plant material, which is horse
The aerial part of bitterroot plant, artemisia argyi plant material are the aerial parts of artemisia argyi plant, and Purslane plants material and argy wormwood are planted
Object material is rinsed well, and naturally dry is crushed with medicinal herb grinder respectively, is crossed 50 meshes, is obtained purslane powder and argy wormwood powder；
2) it extracts: being separately added into ethyl acetate into purslane powder and argy wormwood powder by solid-liquid ratio 1g:20mL, ultrasound mentions
12h is taken, gained filtrate is concentrated 25min at 35 DEG C, respectively obtains purslane extract and argy wormwood mentions by filtering gained extracting solution
Take object；
3) mix: 1:5 mixing purslane extract and biennial wormwood extract in mass ratio form composition.
Fungistatic effect measurement:
Test three kinds of extract combinations to study the synergistic effect between purslane extract and biennial wormwood extract, individually
Embodiment 1 obtained in purslane extract (A group, 10mL purslane extract), argy wormwood obtained in individual embodiment 1
Extract (B group, 50mL biennial wormwood extract), composition (C group, 10mL purslane extract and the 50mL argy wormwood that embodiment 1 obtains
The composition of extract), ginseng root rot opportunistic pathogen spore is bought in Chinese microorganism strain center.
A group bacteriostatic experiment: being made the disk that diameter is 6mm with puncher for qualitative filter paper, and sterilizing will be gone out with aseptic nipper
Filter paper after crossing bacterium is put into A group, impregnates 1h, is air-dried, and is taken 10mL disease fungus spore suspension that high pressure is added respectively and is gone out
Bacterium is simultaneously cooled in about 40 DEG C -50 DEG C of 120mL PDA culture medium, pours into culture medium after mixing and plate is made in culture dish,
For use, it is affixed on the planar surface for having smeared bacteria suspension with aseptic nipper clamping filter paper, and presses lightly on and is allowed to come into full contact with, often
Ware is equidistant to put 4, and each test is 3 times parallel, measures antibacterial circle diameter size after 48h is cultivated in 28 DEG C of biochemical cultivation cases,
It is averaged.
B group bacteriostatic experiment: step is the same as A group.
C group bacteriostatic experiment: step is the same as A group.
Experimental result is as follows:
By Jin's formula it can be concluded that purslane extract and biennial wormwood extract have when inhibiting ginseng root rot opportunistic pathogen
There is high Collaboration effect, there is significant inhibitory effect to ginseng root rot opportunistic pathogen, improve the ability of the anti-root rot of ginseng.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field
Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent
Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (8)
1. the application of purslane and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that purslane extract and biennial wormwood extract
Composition be used to prevent and treat purposes in ginseng root rot.
2. the application of purslane according to claim 1 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
The preparation of composition comprises the steps of:
1) Purslane plants material and artemisia argyi plant material are extracted, separately in a solvent to obtain extracting solution；
2) every kind of extracting solution is separately concentrated to provide purslane extract and biennial wormwood extract；
3) purslane extract and biennial wormwood extract are mixed, composition is formed.
3. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
Solvent is selected from the ethyl alcohol that distilled water, ethyl acetate and mass fraction are 95% in step 1).
4. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
The solid-liquid ratio of solvent and vegetable material is 1g:16-20mL in step 1).
5. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
Ultrasonic extraction, extraction time 10-12h are extracted as in step 1).
6. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
Purslane plants material is the aerial part of Purslane plants in step 1), and artemisia argyi plant material is the overground part of artemisia argyi plant
Point.
7. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
Thickening temperature is 35-40 DEG C in step 2), concentration time 20-25min.
8. the application of purslane according to claim 2 and argy wormwood in prevention and treatment ginseng root rot, it is characterised in that: described
The mass ratio of purslane extract and biennial wormwood extract is 1:5 in step 3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811061156.5A CN109463181A (en) | 2018-09-12 | 2018-09-12 | The application of purslane and argy wormwood in prevention and treatment ginseng root rot |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811061156.5A CN109463181A (en) | 2018-09-12 | 2018-09-12 | The application of purslane and argy wormwood in prevention and treatment ginseng root rot |
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| CN109463181A true CN109463181A (en) | 2019-03-15 |
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| CN201811061156.5A Pending CN109463181A (en) | 2018-09-12 | 2018-09-12 | The application of purslane and argy wormwood in prevention and treatment ginseng root rot |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642399A (en) * | 2015-01-23 | 2015-05-27 | 广西大学 | Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot |
-
2018
- 2018-09-12 CN CN201811061156.5A patent/CN109463181A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642399A (en) * | 2015-01-23 | 2015-05-27 | 广西大学 | Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng root rot |
Non-Patent Citations (4)
| Title |
|---|
| 刘仪等: "《植物病害研究与防治》", 31 March 1998, 中国农业科技出版社 * |
| 廖万清等: "《现代真菌病学》", 31 October 2017, 复旦大学出版社 * |
| 方哲等: ""64种药用植物提取液对3 种药用抑菌活性影响"", 《沈阳农业大学学报》 * |
| 马欣等: ""中草药提取物对蔬菜土传病原真菌的抑制作用"", 《安徽农业科学》 * |
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