CN103053736A - Efficient green fluorine-reducing preparation process of golden-camellia-enriched loose tea - Google Patents
Efficient green fluorine-reducing preparation process of golden-camellia-enriched loose tea Download PDFInfo
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- CN103053736A CN103053736A CN2013100146850A CN201310014685A CN103053736A CN 103053736 A CN103053736 A CN 103053736A CN 2013100146850 A CN2013100146850 A CN 2013100146850A CN 201310014685 A CN201310014685 A CN 201310014685A CN 103053736 A CN103053736 A CN 103053736A
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Abstract
The invention discloses an efficient green fluorine-reducing preparation process of golden-camellia-enriched loose tea. The process comprises the following specific steps of: (1) screening and blending raw dark green tea; (2) performing green fluorine reduction on the raw dark green tea; (3) performing pile fermentation on the raw dark green tea; (4) separating and purifying eurotium cristatum; (5) preparing eurotium cristatum spore suspension with a certain aging degree and determining the concentration of the spore suspension; (6) adding the eurotium cristatum spore suspension to the tea; (7) drying the tea; (8) growing dim on the tea; and (9) drying and preserving the tea. According to the efficient green fluorine-reducing preparation of the golden-camellia-enriched loose tea, pollution-free green fluorine reduction can be realized in the manufacturing period of the golden-camellia-enriched loose tea, and various qualities such as liquor color, aroma, taste and brewing resistance of the golden-camellia-enriched loose tea are not influenced.
Description
Technical field
The present invention relates to technical field of agricultural product process, be specifically related to the loose tea preparation technology of fluorine content in the rich golden-flower loose tea of a kind of efficient reduction, green is fallen fluorine and is referred to fluorine content in the pollution-free reduction Dark Green Tea raw material.
Background technology
Fluorine is one of necessary trace element of human life activity, and the people needs to take in the fluorine of 1.0～1.5 mg every day approximately.An amount of fluorine can promote the calcification of skeleton and tooth, strengthens the intensity of bone.But long-term excess intake fluoride can cause fluorine poisoning, as because the fluorine Excessive Intake can cause the pathological change of the tissues such as tooth, bone, also can cause the lesion tissue of the organs such as kidney, liver etc.
Tea tree is a kind of plant of enrichment fluorine element, and its Leaf is an important place of fluorine element enrichment, because the fluorine content in different every kilogram of tealeaves of tea tree breed, the old tender degree of blade does not wait from tens to thousands of milligrams.The drinking safety problem of the tea that therefore, is caused by fluorine element causes people's attention in recent years gradually.At present, most countries has all been formulated human body fluorine every day intake standard in the world.The proposed standard that the U.S. and Germany propose is adult's daily intake 1.5～4.0 mg; Nippon Standard is 2.1～2 .3 mg; World health group (WHO) regulation, every day, suitable fluorine intake was 2.5～4 .0 mg per capita; The maximum daily intaking amount standard that China formulates is 3.5mg.And should be lower than among 200mg and the GB1996522005 in to the fluoride content of brick tea regulation every kilogram content of fluoride less than 300 mg for every kilogram of test quantity of fluoride in tea compound content that tealeaves industry standard in 2003 NY65922003 of Ministry of Agriculture regulation is arranged before the Content of Fluoride in Tea standard mesh.
Rich golden-flower loose tea is to be that raw material is made with Dark Green Tea, belong to typical black tea, raw material is comparatively thick old, wherein the content of fluorine is quite high, for product quality and the drinking safety that improves tealeaves, guarantee the healthy of the witness people, 1997, former Premier of the State Council De comrade Zhu Rongji has done the written instructions of " will quickly organize the production of the black tea of low fluorine ", and after this state revenue is appropriated for many years special fund and is used for the research and development of the black tea of low fluorine and obtained some impressive progresses.But fluorine content problem does not well solve at present yet, at present, fall the fluorine measure and mainly contain the low fluorine kind of selection, Optimal Cultivation Techniques, improve the tender degree of raw material, fluorine falls in chemistry in the process, fluorine etc. falls in the brick tea water chemistry, from present progress, cultivating low fluorine kind is a chronicity measure, still be difficult to resolve in a short time the black high problem of tea fluorinated volume of determining, and the raising feedstock grade, the burden that certainly will add the heavy user, may cause the part consumer to be run off, in millet paste, add defluorinating agent, although fluoro droping effect is remarkable, but it mostly is greatly chemical reagent, may cause new pollution or cause the millet paste quality to descend in defluorination.Thereby demand developing the green concrete technology that reduces fluorine in the rich golden-flower loose tea of a kind of pollution-free high-efficient urgently.
Summary of the invention
Technical problem to be solved by this invention is: solve the above-mentioned existing problem that the fluorine technology exists of falling, and provide a kind of efficient green to fall the rich golden-flower loose tea preparation technology of fluorine, can realize within the rich golden-flower loose tea making phase that fluorine falls in pollution-free green, and not affect the various qualities such as soup look, tea perfume, tea flavour, degree of enduring repeated infusions of rich golden-flower loose tea.
The technical solution used in the present invention is: the rich golden-flower loose tea preparation technology of fluorine falls in a kind of efficient green, and concrete steps are as follows:
1. Dark Green Tea sub-sieve, assorted;
2. fluorine falls in green;
3. wet heap;
4. separation, the purifying of the loose capsule bacterium of coronoid process;
5. certain Degree of Ageing eurotium cristatum spore suspension preparation is determined with concentration;
6. add eurotium cristatum spore suspension;
7. tea curing;
8. grow dim;
9. dry and preservation.
In the technique scheme, described Dark Green Tea sub-sieve, assortedly carry out according to the Dark Green Tea grade scale; Described green reducing fluoride method is that 80 ℃ clean water immersion was abandoned soak after 4-8 minute; Described wet heap is that 37 ℃ of wet heaps control in 24 hours water content is 28%; The isolation and purification method of the loose capsule bacterium of described coronoid process is: with the dull and stereotyped gradient dilution separation and purification of the PDA Solid agar culture that adds 10% Fu brick tea juice; Described certain Degree of Ageing eurotium cristatum spore suspension preparation with density determination method is: the golden flower bacterium colony of picking purifying, being seeded to the PDA fluid nutrient medium that adds 10% Fu brick tea juice, 20% sucrose and 10%NaCl cultivates, 28 ℃ leave standstill cultivation 5 days, then the temperature that raises was to 30-32 ℃ of cultivation 14-30 days, the concussion dispersal spore, sterilized water dilution spore suspension concentration is controlled at 10
7-10
8Individual/mL; Described interpolation eurotium cristatum spore suspension addition is 0.25 mL/100g; Described tea curing condition is airtight 65-68 ℃, 1 hour; Described grow dim controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then humidity 70%, 28 ℃ of temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong; Described drying and saving as: rich golden-flower loose tea 40-50 ℃ drying indoor preservation of normal temperature after 1 day.
PDA refers to the abbreviation of Potato Dextrose Agar.
The PDA Solid agar culture refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, slightly cold rear with the double gauze filtration, in filtrate, add 15g agar, heat fused, add at last sucrose 20g, mixing is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).
The PDA fluid nutrient medium refers to above-mentioned culture medium process for preparation operation, but does not add agar.
The PDA flat board refers to be cooled to about 50 ℃ after above-mentioned medium sterilization finishes, on superclean bench, culture medium poured into sterilized in advance and dry culture dish in, it is to be cooled that to be the PDA Solid agar culture that makes dull and stereotyped.
The PDA solid medium of 10% Fu brick tea juice refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, slightly cold rear with the double gauze filtration, in filtrate, add the tea juice that 100mL prepares, then add 15g agar, heat fused adds sucrose 20g mixing at last, is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).Be cooled to about 50 ℃ after sterilization finishes, on superclean bench, the PDA Solid agar culture of 10% Fu brick tea juice poured into sterilized in advance and dry culture dish in, the PDA Solid agar culture that is the 10% Fu brick tea juice that makes to be cooled is dull and stereotyped.
The PDA fluid nutrient medium of 10% Fu brick tea juice is identical with the aforesaid operations process, but does not add agar.
Dull and stereotyped gradient dilution method is:
Finished product Fu brick sample is pulverized sample with the plant micro pulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL namely is made into 1 * 10
-1The Fu brick sample solution of g/mL is got 1 mL constant volume, 10 mL and is made into 1 * 10 from mentioned solution
-1The Fu brick sample solution of g/mL is mixed with concentration 1 * 10 successively
-3, 1 * 10
-4, 1 * 10
-5Each 10 mL is for subsequent use for the Fu brick tea sample solution of g/mL.Get 1 * 10
-3, 1 * 10
-4, 1 * 10
-5Each 0.5 mL of the Fu brick sample solution of g/mL is applied on the quick isolation medium flat board of crown loose capsule bacterium with spreader, dries up on the super-clean bench about 15 minutes, be inverted plate, 28 ℃ of cultivations, picking dominant bacteria after 3-4 days---golden flower bacterium, the similarity condition subculture is cultivated until purifying.
The setting-out inoculation refers to separate corresponding microorganism with the bacterium colony picking that it is good that the transfer needle of sterilization will be grown in new solid medium plate setting-out on super-clean bench.
The golden flower bacterium colony refers to that the golden flower bacterium grows into a bacterium colony aggregate that forms certain the time at the solid medium flat board, and bacterium colony refers to the microbic mass that flocks together.
The concrete preparation method of Fu brick tea juice: take by weighing 20g Fu brick tea according to 1g Fu brick tea sample than the ratio of 30mL distilled water, add 600mL distilled water, 100 ℃ of water-baths 2 hours are settled to 600mL with graduated cylinder and are the Fu brick tea juice for preparing after slightly cooling off.
Be made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Refer to concentration, unit is g/mL.Spore suspension concentration 10
7-10
8" individual " among individual/ml refers to the ascospore of single golden flower bacterium.Method of counting is counted or with its OD value of 722 type spectrophotometer measurements, is controlled at about 0.6 for being coated on the gradient dilution method to grow on the PDA Solid agar culture flat board that adds 10% Fu brick tea juice, makes golden flower spore suspension concentration be controlled at every milliliter 10
7-10
8Individual golden flower spore.
Above-mentioned golden flower is the loose capsule bacterium of coronoid process.
The invention provides a kind of efficient green and fall the rich golden-flower loose tea preparation technology of fluorine, can realize within the rich golden-flower loose tea making phase that fluorine falls in pollution-free green, and do not affect the various qualities such as soup look, tea perfume, tea flavour, degree of enduring repeated infusions of rich golden-flower loose tea.
Description of drawings
Fig. 1 is that the present invention cultivates the loose capsule bacterium colony photo of the coronoid process that separated purifying in 7 days;
Fig. 2 is 20 days cultivation results photos of spore suspension of the loose capsule bacterium of coronoid process of the present invention;
Fig. 3 is that the rich golden-flower loose tea of the fluorine as a result photo that grows dim falls in product, superfine product;
Fig. 4 is that product, superfine product fall the rich golden-flower loose tea of fluorine and repeatedly brew design sketch;
Fig. 5 is that green is fallen fluorine to fluorine content, Tea Polyphenols, water extraction, caffeine composition influence evaluation table in the rich golden-flower loose tea.
The specific embodiment
1. Dark Green Tea sub-sieve, assorted:
According to traditional Dark Green Tea grade scale raw material is carried out sub-sieve, assorted conveniently to be pressed into the Fu brick tea of corresponding different class.
2. fluorine falls in green:
With clean water, 80 ℃ of water temperatures are kept to soak and were drained after (deciding according to the tender degree of raw material) in 4-8 minute, abandon soak.80 ℃ of one-level Dark Green Tea water temperatures are kept and were soaked 4 minutes, 80 ℃ of secondary Dark Green Tea water temperatures are kept and were soaked 5 minutes, 80 ℃ of three grades of Dark Green Tea water temperatures are kept and were soaked 6 minutes, and 80 ℃ of level Four Dark Green Tea water temperatures are kept and soaked 7 minutes, and 80 ℃ of Pyatyi Dark Green Tea water temperatures are kept and soaked 8 minutes.
3. wet heap;
37 ℃ of wet heaps 24 hours, control water content are 28%.
4. the loose capsule bacterium of coronoid process is separation, the purifying of golden flower:
The loose capsule bacterium of coronoid process is that golden flower separates from Yiyang tea processing factory finished product Hunan benefit board Fu brick sample.Concrete separate and purge process is: finished product Hunan benefit board Fu brick sample is pulverized, with dull and stereotyped gradient dilution method on the PDA Solid agar culture flat board of interpolation 10% Fu brick tea juice 28 ℃ of cultivations until purifying.
5. the preparation of the spore suspension of certain Degree of Ageing golden flower is determined with concentration:
The golden flower bacterium colony of picking purifying, be seeded to the PDA fluid nutrient medium that adds 10% Fu brick tea juice, 20% sucrose and 10%NaCl and cultivate, 28 ℃ leave standstill cultivation 5 days, and the temperature that then raises was to 30-32 ℃ of cultivation 14-30 days, the concussion dispersal spore, sterilized water dilution spore suspension concentration is controlled at 10
7-10
8Individual/mL.
6. interpolation eurotium cristatum spore suspension:
After 100 ℃ of sterilization coolings in 2 hours of Dark Green Tea, according to the ratio that every 100g Dark Green Tea adds the 0.25mL eurotium cristatum spore suspension, add mixing with the form of spraying.
7. tea curing:
The tea curing condition is airtight 65-68 ℃, 1 hour.
8. grow dim:
Controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then humidity 70%, 28 ℃ of temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong.
9. dry and preservation:
The indoor preservation of normal temperature after 1 day of rich 40-50 ℃ of drying of golden-flower loose tea.
Embodiment 1: golden flower is separation, the purifying of the loose capsule bacterium of rostrum:
Finished product Hunan benefit board Fu brick sample is pulverized with the plant micro pulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL is made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Each 10mL gets 10
-3, 10
-4, 10
-5The PDA Solid agar culture flat board of 10% Fu brick tea juice is added in three each 0.0.5mL coatings of gradient, dry up on the super-clean bench, cultivated 3 days for 28 ℃, the golden yellow dominant colony of picking, setting-out is to the PDA Solid agar culture flat board that adds 10% Fu brick tea juice, and the similarity condition subculture is cultivated until purifying.
The golden flower bacterium colony of separation and purification is seen accompanying drawing 1.
Embodiment 2: the spore suspension preparation of the loose capsule bacterium spore of coronoid process:
The golden flower bacterium colony of picking purifying, being seeded to the PDA fluid nutrient medium that adds 10% Fu brick tea juice, 20% sucrose and 10%NaCl cultivates, 28 ℃ leave standstill cultivation 5 days, then the temperature that raises was to 30-32 ℃ of cultivation 14-30 days, concussion disperses conidium, uses the double-layer sterile filtered through gauze, and rear 280rpm shaken cultivation is disperseed conidium again, sterilized water dilution spore suspension to OD value is 0.6, and spore suspension concentration is controlled at 10
7-10
8Individual/ml, be available eurotium cristatum spore suspension.
The eurotium cristatum spore suspension cultivation results is seen accompanying drawing 2.
Embodiment 3: the checking of growing dim of the rich golden-flower loose tea of fluorine falls in efficient green
Get respectively Yiyang tea processing factory one product Fu brick raw material, each 10Kg of superfine product Fu brick raw material, not carry out green fluorine falls in contrast, according to black tea quality and the fermentation key technology control production, teaching ﹠ research Demonstration Base experiment of growing dim in the Hunan Province of 1-9 step in the specific embodiment, it falls the low energy of fluorine rate and reaches 50%, the growth generally luxuriant, lovely luster is golden yellow, particle is loose, arohid flavour is strong.Brewing presentation of results soup look does not almost have difference with contrast, and steeping equally ten times soup looks constant, and taste is pureer and milder, without the astringent taste of newly picked and processed tea leaves; Testing result shows, fall in the rich golden-flower loose tea after fluorine is processed Tea Polyphenols, water extraction, caffeine composition compared with the control through water logging, slippage is very faint, and taste is compared in contrast, can not taste obvious change, so can say rich golden-flower loose tea quality impact very little.
One product, superfine product fall the rich golden-flower loose tea of fluorine and grow dim and the results are shown in accompanying drawing 3.
One product, superfine product fall the rich golden-flower loose tea of fluorine and brew effect ten times and see Fig. 4.
Green is fallen fluorine to fluorine content, Tea Polyphenols, water extraction, caffeine composition influence evaluation table in the rich golden-flower loose tea, sees Fig. 5.
Claims (2)
1. the rich golden-flower loose tea preparation technology of fluorine falls in an efficient green, and it is characterized in that: concrete steps are as follows:
1. Dark Green Tea sub-sieve, assorted;
2. fluorine falls in green;
3. wet heap;
4. separation, the purifying of the loose capsule bacterium of coronoid process;
5. certain Degree of Ageing eurotium cristatum spore suspension preparation is determined with concentration;
6. add eurotium cristatum spore suspension;
7. tea curing;
8. grow dim;
9. dry and preservation.
2. the rich golden-flower loose tea preparation technology of fluorine falls in efficient green according to claim 1, it is characterized in that: described Dark Green Tea sub-sieve, assortedly carry out according to the Dark Green Tea grade scale; Described green reducing fluoride method is that 80 ℃ clean water immersion was abandoned soak after 4-8 minute; Described wet heap is that 37 ℃ of wet heaps control in 24 hours water content is 28%; The isolation and purification method of the loose capsule bacterium of described coronoid process is: with the dull and stereotyped gradient dilution separation and purification of the PDA Solid agar culture that adds 10% Fu brick tea juice; Described certain Degree of Ageing eurotium cristatum spore suspension preparation with density determination method is: the golden flower bacterium colony of picking purifying, being seeded to the PDA fluid nutrient medium that adds 10% Fu brick tea juice, 20% sucrose and 10%NaCl cultivates, 28 ℃ leave standstill cultivation 5 days, then the temperature that raises was to 30-32 ℃ of cultivation 14-30 days, the concussion dispersal spore, sterilized water dilution spore suspension concentration is controlled at 10
7-10
8Individual/mL; Described interpolation eurotium cristatum spore suspension addition is 0.25 mL/100g; Described tea curing condition is airtight 65-68 ℃, 1 hour; Described grow dim controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then humidity 70%, 28 ℃ of temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong; Described drying and saving as: rich golden-flower loose tea 40-50 ℃ drying indoor preservation of normal temperature after 1 day.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105230827A (en) * | 2015-10-13 | 2016-01-13 | 杭州艺福堂茶业有限公司 | Eurotium cristatum tea automatic fermentation process |
| CN109136098A (en) * | 2018-07-20 | 2019-01-04 | 石海滨 | A kind of culture medium for being suitable for being separately cultured coronoid process dissipate capsule bacterium and method |
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| CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
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2013
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| CN1802972A (en) * | 2006-01-25 | 2006-07-19 | 中南大学 | Production method of low fluorine, aluminium instant red tea |
| CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230827A (en) * | 2015-10-13 | 2016-01-13 | 杭州艺福堂茶业有限公司 | Eurotium cristatum tea automatic fermentation process |
| CN105230827B (en) * | 2015-10-13 | 2019-01-04 | 杭州艺福堂茶业有限公司 | A kind of eurotium cristatum tea automation zymotechnique |
| CN109136098A (en) * | 2018-07-20 | 2019-01-04 | 石海滨 | A kind of culture medium for being suitable for being separately cultured coronoid process dissipate capsule bacterium and method |
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