CN102084903A - Preparation process of fungus-grown loosened green tea - Google Patents
Preparation process of fungus-grown loosened green tea Download PDFInfo
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- CN102084903A CN102084903A CN 201010598193 CN201010598193A CN102084903A CN 102084903 A CN102084903 A CN 102084903A CN 201010598193 CN201010598193 CN 201010598193 CN 201010598193 A CN201010598193 A CN 201010598193A CN 102084903 A CN102084903 A CN 102084903A
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Abstract
The invention provides a preparation process of fungus-grown loosened green tea, which comprises the steps of: 1, purifying and separating eurotium cristatum; 2, preparing an eurotium cristatum spore suspension and determining the concentration thereof; 3, processing a green tea sample; 4, adding the eurotium cristatum suspension; 5, drying the tea, 6, controlling the humidity and temperature for fungus growing; 7, ageing and drying the fungus-grown loosened green tea; and 8, storing the fungus-grown loosened green tea. The invention can realize the fungus growing of the green tea by inoculating by using the golden flower fungus in Fuzhuan tea in a short period, and golden flower is generally flourish, bright and yellow in color, and large in particles, thus the fungus-grown loosened green tea inherits the impressions of the traditional green tea and can be endowed with the characteristics of the Fuzhuan tea, and the internal quality and the additional value of the green tea are improved.
Description
Technical field:
The present invention relates to technical field of agricultural product process, be specifically related to traditional green tea is carried out after fermentation processing and makes the grow dim preparation technology of loose tea of green tea.Described flower is the diffusing capsule bacterium of coronoid process, is commonly called as golden flower.
Background technology:
Green tea claims azymic tea again, is to be raw material with suitable tea tree young sprout, through the tealeaves that completes, kneads, typical process process such as drying is made.Its do dark brown pool and brew after millet paste, leaf at the bottom of be homophony with the green, so.The characteristic of green tea, more reservation the natural materials in the bright leaf.Wherein the Tea Polyphenols caffeine keeps more than 85% of bright leaf, and chlorophyll keeps about 50%, and vitamin loss is also less, thereby has formed the characteristics of green tea " clear soup greenery, the flavour convergence is strong ".In the Chinese green tea, famous-object is maximum, and not only fragrant high flavor is long, excellent quality, and moulding uniqueness, have higher value of art appreciation, green tea is different by its drying and fixing method, generally is divided into fried green, baking green grass or young crops, shines green grass or young crops and steaming green tea, but literature search shows that traditional green tea is carried out after fermentation improves still blank of its interior quality research, local flavor characteristics to traditional green tea is given its Fu brick tea not only can increase the added value of green tea, and have promoted the interior quality of green tea, black tea.
Tea promptly is called as " medicines of ten thousand diseases " at ancient times.Especially to the present age, factors such as fat surplus, radiation, environmental pollution and pressure multiplier cause many modern diseases such as hypertension, high fat of blood, coronary heart disease, diabetes, hypercholesterolemia, cancer and mental handicape etc., and the Fu brick tea all has certain curative effect and prevention effect to this.Modern scientific research shows, the Fu brick tea to the pharmacological function of human body mainly be because tealeaves contain the number of chemical composition and found at present hundreds of trace elements and microorganism arranged.Particularly, the exclusive coronoid process capsule bacterium that looses in the Fu brick tea, after people drank tea, these active ingredients were also absorbed by human body thereupon, thereby brought a lot of benefits for people's health.By animal experiment, the human clinical trial proves conclusively the Fu brick tea and have significant lowering blood-fat and reducing weight and hypoglycemic effect equally in experimental animal and human body, and strict toxicological assessment shows that it is safe that long-term high dose is drunk Fu tea.The epidemiology survey that carry out Fu brick tea emphasis consumption areas such as western part of China Xinjiang, Inner Mongol, Qinghai shows, long-term drinking Fu tea has good regulating action to blood fat, blood sugar, the blood pressure of human body, body weight, the bodily form are had good regulating and controlling effect, and can significantly strengthen human body constitution, delay senility.The Fu brick tea was the indispensable important beverage of borderland ethnic group in the past, and existing also coming into vogue interiorly drunk the Fu brick tea, but because its raw material---black wool tea is comparatively thick old, and particularly fluorinated volume is higher, adds the characteristics on the impression, and interior ground acceptance level is popularized far away.
Summary of the invention:
Technical problem to be solved by this invention is: solve the problem that above-mentioned prior art exists, and provide a kind of green tea loose tea preparation technology that grows dim, realize that with the inoculation of the golden flower bacterium in the Fu brick tea green tea grows dim in a short time, and golden flower is generally luxuriant, lovely luster is golden yellow, particle is loose, the impression characteristics of traditional green tea had both been inherited, can give its Fu brick tea characteristics again, promote its inherent quality and added value.
The technical solution used in the present invention is: the loose tea preparation technology that grows dim the steps include: this green tea
1) purifies and separates of the diffusing capsule bacterium of coronoid process;
2) coronoid process looses, and capsule bacterium spore suspension prepares and concentration is determined;
3) processing of green tea sample;
4) add the diffusing capsule bacteria suspension of coronoid process;
5) tea curing;
6) controlled humidity and temperature are grown dim;
7) green tea ageing, the drying of loose tea of growing dim;
8) the green tea preservation of loose tea of growing dim.
In the technique scheme, the purifies and separates of the diffusing capsule bacterium of described coronoid process is: separate with the PDA agar solid medium culture purified of adding 10% tea juice;
The diffusing capsule bacterium spore suspension preparation of coronoid process prepares with the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl; Spore suspension concentration is determined 10
7-10
8Individual/mL;
The processing controls humidity of green tea sample is about 28%;
Golden flower suspension addition is 0.5mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour;
Controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, 26 ℃ of temperature, and humidity 70% then, and it is generally luxuriant that 28 ℃ of temperature are grown until golden flower, and bright in colour golden yellow, the particle hypertrophy is till arohid flavour is strong; Humidity drops to 60% gradually then, temperature is increased to 36 ℃, green tea ageing 7 days gradually; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
PDA refers to that the abbreviation Chinese implication of Potato Dextrose Agar is: potato dextrose agar.
PDA agar solid medium refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, filter with double gauze cold slightly back, adds 15g agar in filtrate, heat fused, add sucrose 20g at last, mixing is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).
The PDA fluid nutrient medium refers to by above-mentioned culture medium process for preparation operation, but does not add agar.
The PDA flat board refers to be cooled to about 50 ℃ after above-mentioned PDA agar solid medium sterilization finishes, and culture medium is poured in the also dry culture dish of having sterilized in advance into the PDA agar solid medium flat board that makes that is to be cooled on superclean bench.
Improvement PDA solid medium refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, filter with double gauze cold slightly back, in filtrate, add the tea juice that 100mL prepares, add 15g agar then, heat fused adds sucrose 20g at last, mixing, be settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).Sterilization is cooled to about 50 ℃ after finishing, and the PDA agar solid medium of 10% tea juice is poured in the also dry culture dish of having sterilized in advance into the PDA agar solid medium flat board that is the 10% tea juice that makes to be cooled on superclean bench.
Improvement PDA fluid nutrient medium is meant by above-mentioned culture medium process for preparation operation, but does not add agar.
Dull and stereotyped gradient dilution method is: finished product Fu brick tea sample is pulverized sample with the plant micropulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL promptly is made into 1 * 10
-1The Fu brick sample solution of g/mL is got 1mL constant volume 10mL and is made into 1 * 10 from above-mentioned solution
-2The Fu brick sample solution of g/mL is mixed with concentration 1 * 10 according to this
-3, 1 * 10
-4, 1 * 10
-5Each 10mL of Fu brick sample solution of g/mL is standby.Get 1 * 10
-3, 1 * 10
-4, 1 * 10
-5Each 0.5mL of Fu brick sample solution of g/mL is applied on the quick isolation medium flat board of crown diffusing capsule bacterium with spreader, dries up on the super-clean bench about 15 minutes, is inverted plate, 28 ℃ of cultivations, and picking dominant bacteria after 3-4 days---golden flower similarity condition is cultivated until purifying.
The setting-out inoculation refers to separate corresponding microorganism with the bacterium colony picking that it is good that the transfer needle of sterilization will be grown in new solid medium plate setting-out in super-clean bench.
The golden flower bacterium colony refers to that the golden flower bacterium grows into a bacterium colony aggregate that forms certain the time on the 10%PDA culture medium flat plate, fall to being meant the microbic mass that flocks together.
The preparation method of tea juice is: take by weighing 20g Fu brick tea according to 1g Fu brick tea sample than the ratio of 30mL distilled water, add 600mL distilled water, 100 ℃ of water-baths 2 hours are treated to cool off slightly the back and are settled to 600mL with graduated cylinder and are the tea juice for preparing.
Be made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Be meant concentration, unit is g/mL.
Spore concentration at outstanding night 10
7-10
8" individual " among individual/m l is meant the ascospore of single golden flower bacterium.Method of counting is counted or with its OD value of 722 type spectrophotometer measurements, is controlled at about 0.6-0.8 for being coated on the gradient dilution method to grow on the PDA agar solid medium flat board that adds 10% tea juice, makes golden flower spore suspension concentration be controlled at every milliliter 10
7-10
8Individual golden flower spore, above-mentioned OD value is a light absorption value.
The present invention (3-4 days) in a short time realizes that with the inoculation of the golden flower bacterium in the Fu brick tea green tea grows dim, and golden flower is generally luxuriant, and is bright in colour golden yellow, the particle hypertrophy, both inherited the impression characteristics of traditional green tea, and can give its Fu brick tea characteristics again, promote interior quality and added value.For traditional green tea carries out after fermentation processing, makes the green tea loose tea that grows dim a kind of new technology new technology is provided.
Description of drawings:
Fig. 1 is the diffusing capsule bacterium colony photo of the coronoid process of separation and purification of the present invention after 7 days;
Fig. 2 cultivates 7 days photos as a result for the diffusing capsule bacterium spore suspension of coronoid process of the present invention;
Fig. 3 is the brand 1 green tea loose tea photo as a result that grows dim;
Fig. 4 is the brand 2 green tea loose tea photo as a result that grows dim.
The specific embodiment
1, the separation of golden flower, purifying:
The capsule bacterium (being golden flower) that looses separates from the benefit board Fu brick sample of Yiyang tea processing factory finished product Hunan coronoid process.Concrete separate and purge process is: finished product Hunan benefit board Fu brick sample is pulverized, with dull and stereotyped gradient dilution method on the PDA flat board of interpolation 10% tea juice 28 ℃ of cultivations until purifying.
2, the preparation of the spore suspension of golden flower is determined with concentration:
The golden flower bacterium colony of picking purifying is seeded to and adds in the improvement PDA fluid nutrient medium, cultivates 6-7 days, and makes spore suspension concentration be controlled at 10 for 28 ℃
7-10
8Individual/mL.
3, the processing of green tea sample:
Steam 10 minutes softening green tea with high-temperature steam, controlled humidity is 28% simultaneously.
4, add the golden flower spore suspension:
According to the ratio that every 100g tea adds 0.5mL golden flower spore suspension, add with the form of spraying, fully mixing.
5, tea curing:
65-68 ℃ in confined conditions of tea behind interpolation golden flower spore suspension and the mixing, baking 1 hour.
6, controlled humidity and temperature are grown dim:
Controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then 28 ℃ of humidity 70%, temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong.
7, the green tea ageing of loose tea of growing dim:
Humidity drops to 60% gradually, temperature is increased to 36 ℃, green tea ageing 7 days gradually.Dry and the preservation of 8 loose teas:
Loose tea 40-50 ℃ drying is after 1 day, the indoor preservation of normal temperature.
Embodiment 1: the separation of golden flower, purifying:
Finished product Hunan benefit board Fu brick tea sample is pulverized with the plant micropulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10ml is made into concentration 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Each 10mL gets 10
-3, 10
-4, 10
-5Three each 0.0.5mL of gradient coat the PDA flat board that adds 10% tea juice, dry up on the super-clean bench, and be inverted 28 ℃ and cultivated 3 days, the golden yellow dominant colony of picking, setting-out is to the PDA flat board that adds 10% tea juice, and similarity condition continues to cultivate until purifying.
The golden flower bacterium colony of separation and purification is seen accompanying drawing 1.
Embodiment 2: the spore suspension preparation of golden flower:
The golden flower bacterium colony of picking purifying, be seeded in the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl, 28 ℃ leave standstill cultivation 2 days, the temperature that raises then was to 30-32 ℃ of cultivation 7-10 days,, the 280rpm shaken cultivation is disperseed conidium then, filters with double gauze, recording the OD value with 722 type spectrophotometers is 0.6-0.8, is available golden flower spore suspension.
The spore suspension cultivation results of golden flower is seen accompanying drawing 2.
Embodiment 3: the checking that the green tea loose tea grows dim:
Get two kinds of each 1Kg of green tea of commercially available different brands respectively, carry out loose tea according to the 2-8 step in the specific embodiment and ferment.
The green tea loose tea grows dim and the results are shown in accompanying drawing 3, Fig. 4.
Claims (6)
1. green tea loose tea preparation technology that grows dim is characterized in that step is:
1) purifies and separates of the diffusing capsule bacterium of coronoid process;
2) coronoid process looses, and capsule bacterium spore suspension prepares and concentration is determined;
3) processing of green tea sample;
4) add the diffusing capsule bacteria suspension of coronoid process;
5) tea curing;
6) controlled humidity and temperature are grown dim;
7) green tea ageing, the drying of loose tea of growing dim;
8) the green tea preservation of loose tea of growing dim.
2. the green tea according to claim 1 loose tea preparation technology that grows dim is characterized in that: the loose purifies and separates of capsule bacterium of described coronoid process is: separate with the PDA agar solid medium culture purified of adding 10% tea juice;
3. the green tea according to claim 1 loose tea preparation technology that grows dim is characterized in that: coronoid process looses the preparation of capsule bacterium spore suspension with the PDA fluid nutrient medium preparation of adding 10% tea juice, 20% sucrose, 10%NaCl; Spore suspension concentration is determined 10
7-10
8Individual/mL;
4. the green tea according to claim 1 loose tea preparation technology that grows dim, it is characterized in that: the processing controls humidity of green tea sample is about 28%;
5. the green tea according to claim 1 loose tea preparation technology that grows dim, it is characterized in that: golden flower suspension addition is 0.5mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour;
6. the green tea according to claim 1 loose tea preparation technology that grows dim, it is characterized in that: controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, 26 ℃ of temperature, humidity 70% then, temperature is grown generally luxuriant for 28 ℃ until golden flower, bright in colour golden yellow, the particle hypertrophy is till arohid flavour is strong; Humidity drops to 60% gradually then, temperature is increased to 36 ℃, green tea ageing 7 days gradually; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
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Cited By (16)
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|---|---|---|---|---|
| CN102640814A (en) * | 2012-05-14 | 2012-08-22 | 咸阳泾渭茯茶有限公司 | Method for making golden flower loose tea without inoculation |
| CN102860389A (en) * | 2012-05-23 | 2013-01-09 | 华中农业大学 | Lotus leaf eurotium cristatum tea and preparation method thereof |
| CN102907514A (en) * | 2012-10-30 | 2013-02-06 | 陕西思尔生物科技有限公司 | Preparation method of golden flower fungus fermented tea tree mushroom drink |
| CN103070267A (en) * | 2013-01-16 | 2013-05-01 | 湖南城市学院 | Floating preparation process of eurotium cristatum-rich lotus leaf tea |
| CN103168866A (en) * | 2013-04-02 | 2013-06-26 | 益阳茶厂有限公司 | Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method |
| CN103340251A (en) * | 2013-06-13 | 2013-10-09 | 陈树杰 | Preparation method for health care tea used for preventing and treating hyperlipidemia |
| CN103749800A (en) * | 2014-01-24 | 2014-04-30 | 南京融点食品科技有限公司 | Method for producing Fu brick tea concentrated solution by using green tea |
| CN104543056A (en) * | 2014-12-31 | 2015-04-29 | 四川省文君茶业有限公司 | Fuzhuan tea processing method with green tea used as blank |
| CN105420020A (en) * | 2015-12-15 | 2016-03-23 | 贵州大学 | Method for producing tea wine by utilizing eurotium cristatus |
| CN105851299A (en) * | 2016-04-21 | 2016-08-17 | 贵州梵锦茶业有限公司 | Method for preparing black brick tea from black tea |
| CN108029829A (en) * | 2017-12-29 | 2018-05-15 | 中南林业科技大学 | A kind of paper mulberry golden flower tea preparation methods |
| CN109645159A (en) * | 2017-10-11 | 2019-04-19 | 南京农业大学 | A method of utilizing the black loose tea of the high-quality floating of southern Jiangsu finished product Green Tea Processing |
| CN109997930A (en) * | 2019-04-15 | 2019-07-12 | 益阳冠隆誉黑茶发展有限公司 | Tealeaves nature fungus growing process, tea Processing method and equipment under non-compaction |
| CN110178929A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of processing method of Pilochun (a green tea) Fu tea floating |
| CN110178934A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of Longjing tea Fu tea floating and preparation method thereof |
| CN113142341A (en) * | 2021-02-04 | 2021-07-23 | 广东省农业科学院茶叶研究所 | Making process of dried orange peel golden flower green tea with blood sugar reducing effect |
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| CN102640814A (en) * | 2012-05-14 | 2012-08-22 | 咸阳泾渭茯茶有限公司 | Method for making golden flower loose tea without inoculation |
| CN102640814B (en) * | 2012-05-14 | 2014-02-12 | 咸阳泾渭茯茶有限公司 | Method for making golden flower loose tea without inoculation |
| CN102860389A (en) * | 2012-05-23 | 2013-01-09 | 华中农业大学 | Lotus leaf eurotium cristatum tea and preparation method thereof |
| CN102860389B (en) * | 2012-05-23 | 2015-01-07 | 华中农业大学 | Lotus leaf eurotium cristatum tea and preparation method thereof |
| CN102907514A (en) * | 2012-10-30 | 2013-02-06 | 陕西思尔生物科技有限公司 | Preparation method of golden flower fungus fermented tea tree mushroom drink |
| CN102907514B (en) * | 2012-10-30 | 2013-10-23 | 陕西思尔生物科技有限公司 | Preparation method of golden flower fungus fermented tea tree mushroom drink |
| CN103070267A (en) * | 2013-01-16 | 2013-05-01 | 湖南城市学院 | Floating preparation process of eurotium cristatum-rich lotus leaf tea |
| CN103168866A (en) * | 2013-04-02 | 2013-06-26 | 益阳茶厂有限公司 | Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method |
| CN103340251A (en) * | 2013-06-13 | 2013-10-09 | 陈树杰 | Preparation method for health care tea used for preventing and treating hyperlipidemia |
| CN103749800A (en) * | 2014-01-24 | 2014-04-30 | 南京融点食品科技有限公司 | Method for producing Fu brick tea concentrated solution by using green tea |
| CN104543056A (en) * | 2014-12-31 | 2015-04-29 | 四川省文君茶业有限公司 | Fuzhuan tea processing method with green tea used as blank |
| CN105420020A (en) * | 2015-12-15 | 2016-03-23 | 贵州大学 | Method for producing tea wine by utilizing eurotium cristatus |
| CN105420020B (en) * | 2015-12-15 | 2018-07-06 | 贵州大学 | A kind of method using coronoid process dissipate capsule bacterium production tea wine |
| CN105851299A (en) * | 2016-04-21 | 2016-08-17 | 贵州梵锦茶业有限公司 | Method for preparing black brick tea from black tea |
| CN109645159A (en) * | 2017-10-11 | 2019-04-19 | 南京农业大学 | A method of utilizing the black loose tea of the high-quality floating of southern Jiangsu finished product Green Tea Processing |
| CN108029829A (en) * | 2017-12-29 | 2018-05-15 | 中南林业科技大学 | A kind of paper mulberry golden flower tea preparation methods |
| CN108029829B (en) * | 2017-12-29 | 2021-05-11 | 中南林业科技大学 | Preparation method of paper mulberry golden camellia |
| CN109997930A (en) * | 2019-04-15 | 2019-07-12 | 益阳冠隆誉黑茶发展有限公司 | Tealeaves nature fungus growing process, tea Processing method and equipment under non-compaction |
| CN110178929A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of processing method of Pilochun (a green tea) Fu tea floating |
| CN110178934A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of Longjing tea Fu tea floating and preparation method thereof |
| CN113142341A (en) * | 2021-02-04 | 2021-07-23 | 广东省农业科学院茶叶研究所 | Making process of dried orange peel golden flower green tea with blood sugar reducing effect |
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