CN103283881A - Solid fermentation process of golden flower white tea - Google Patents
Solid fermentation process of golden flower white tea Download PDFInfo
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Abstract
The invention provides a solid fermentation process of golden flower white tea. The solid fermentation process is characterized in that a method for inoculating eurotium cristatum on white tea in a fermentation process of a solid fermentation pot realizes non-pollution blooming of the white tea so as to prepare the golden flower white tea growing with flourish and uniform golden flower bacteria and a blooming flowering phase is controlled within 7 days. The golden flower white tea prepared by the solid fermentation process disclosed by the invention is more in golden flower bacteria quantity and is golden in tea leaves; the golden flower bacteria quantity in each gram of dry tea leaves is more than 107; the golden flower bacteria are firstly planted in the white tea and the solid fermentation pot is firstly used for manufacturing the white tea; the liquid color is golden yellow, the aroma is pure and the taste is pure and mild; the quality of the white tea is further improved; the solid fermentation process is good for people to further approve and accept the white tea.
Description
Technical field
The present invention relates to a kind of white tea, be specially the solid fermentation process of the white tea of golden flower, belong to white tea processing technique field.
Background technology
The golden flower bacterium, namely the diffusing capsule bacterium of coronoid process is found in the Fu brick tea." golden flower " that has in the Fu brick tea (coronoid process loose capsule bacterium) is that it is exclusive, and its belongs to after fermentation tea, full fermented tea.Modern scientific research, and show by zoopery, human clinical's experiment, toxicologic study, safety evaluatio, epidemiology survey through the authoritative expert: the multiple extraction composition of Fu brick tea " golden flower " all has activation to PPAR γ, PPAR δ, " golden flower " interior two kinds of new active material Fu theine A and Fu theine B have effects such as remarkable reduction human body class fatty compound, blood fat, blood pressure, blood sugar, cholesterol.Long-term drinking Fu tea can promote to regulate metabolism, strengthens human body constitution, delays senility, and human body is played effective pharmacology health care and pathology prevention effect
White tea is one of six big teas, and tea is the Fujian special product in vain, the major production areas in the Fuding, have stable political situation, ground such as Songxi, Jianyang.Basic technology comprise wither, cure (or drying in the shade), choose pick, operation such as multiple fire.Withering is the critical process that forms white tea quality.White tea set has profile bud milli complete, drapes over one's shoulders milli all over the body, and milli is fragrant clear bright, and the soup look yellowish green is limpid, light time of flavour sweet quality characteristic.Belonging to slight fermented tea, is the special treasure in China's teas.Sampling tea because of its one-tenth mostly is the bud head, completely drapes over one's shoulders pekoe, as silver like snow and gain the name.In addition, the white tea of the white tea in the Anji in Chinese Zhejiang and Zhengan County, Guizhou is different from the general white tea that has white fine hair because natural variation full wafer tealeaves is white in color.
The effect of white tea and effect one: white tea anti-cancer, anticancer, heatstroke prevention, detoxify, control toothache, especially old white tea can be used as child's measly antipyretic, and its effect of bringing down a fever is better than antibiotic.
The effect of white tea and effect two: the health protection tea market survey shows, white tea is except containing the intrinsic nutritional labeling of other tealeaves, also contain the necessary organized enzyme of human body, the white tea of the proof long-term drinking of medical research both at home and abroad can significantly improve body lactonase (lipoproteinlipase) activity, promote catabolism of fat, effectively control amount of insulin secretion, delay the intestinal absorption of grape Portugal powder, the sugar that the decomposer inner blood is unnecessary promotes blood glucose balance.White tea contains and enriches several amino acids, and it is cold, the drive away summer heat merit of detoxifcation that having brings down a fever, and sob one cup of white peony tea summer in the producing region, and the few can suffer heatstroke.Therefore white peony is one of white tea of local tea grower's indispensability in summer.In the North China and the place of production, the Fujian good medicine that extensively is considered as treating maintenance measles patient.
The effect of white tea and effect three: the white tea resting period is more long, and its medical value is higher.It is former also to contain abundant vitamin a in the white tea, after it is absorbed by the body, can be converted into vitamin a rapidly, and vitamin a can synthesize rhodopsin, can make eyes see that under half-light thing is clearer, can prevent yctalopia and scheroma.Simultaneously white tea also has radio-opaque substance, and the hematopoietic function of human body is had significant protective effect, can reduce the harm of TV radiation.Therefore drink some white tea in seeing the TV process is to have everything to gain and nothing to lose more, and especially children more should advocate and drink white tea more, are conducive to protect eyes, are healthy and strong.
The effect of white tea and effect four: the flavonoids natural materials such as dibydro myricetrin that white sifting tea is rich in.When the alcohol excess intake, when surpassing human liver's metabolic capability and detoxification ability, alcohol will produce directly or indirectly infringement to liver cell, stimulate that fat is synthetic, anoxic, generation acetaldehyde and induce each relevant enzyme activity and cause upsetting liver metabolism etc.Cause a series of clinical symptoms, cause the generation of alcoholic hepatitis, fatty liver, liver fibrosis so that cirrhosis even liver cancer etc.In addition, the metabolin acetaldehyde of ethanol is to cause the second day after drinking main cause dizzy and evil is liquor-saturated, is the main matter of bringing infringement to liver.
Summary of the invention
The objective of the invention is to be to disclose a kind of process for making of white tea, the solid fermentation process of the white tea of golden flower is provided, guarantee the quality of growing dim of white tea, fermentation back golden flower bacterium is luxuriant, and golden flower bacterium quantity is greater than 10 in every gram stem tea
7Individual.This technology is implanted the golden flower bacterium first in white tea.Utilize solid-state fermentation tank to get zymotechnique, improved grow dim quality and quantity, simplified the step of growing dim, shortened the phase of growing dim, and stopped the possibility of the white tea of living contaminants.
Technical scheme of the present invention is: the solid fermentation process of the white tea of golden flower, by the diffusing capsule bacterium of artificial infection coronoid process, adopt advanced solid-fermented technique to produce the white tea of golden flower.
The solid fermentation process of the white tea of described golden flower is specific as follows:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, similitude requires could determine more than 99.5% to be the diffusing capsule bacterium of coronoid process, could be as bacterial classification
2, white tea charging: will handle the white tea that obtains according to the white tea production technology of tradition and place solid-state fermentation tank, stocking volume is 30% ~ 90% of fermentation tank volume
3, white tea gas steams: steam sterilizing, and be warming up to 90 ~ 130 ℃ and steam 10 ~ 30 min, the solid-state fermentation tank rotating speed is 100-200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.1 ~ 10% white tea tea juice, adds concentration and be 1 ~ 40% glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
4~ 1x10
7Individual every milliliter, evenly add the went out Bai Chashang of bacterium of step 3, make the water content control of white tea 15 ~ 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 100-200 rpm
5. fermentation: keep the water content 15 ~ 100% of tealeaves, the temperature of growing dim is 25 ~ 32 ℃, humidity 70 ~ 86%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 ~ 120 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 5 ~ 7 days processes of growing dim; Detect after the fermentation ends glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content, require residual glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content be no more than 1% of dry weight of tea leaves
6. dry: the white tea of golden flower (pulverizing) with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea; The tea dust (tea dust diameter 100um-1cm) of pulverizing the white tea in back in various degree can be packaged into tea bag after fermentation, drying, or makes health products.
The present invention has broken loaded down with trivial details traditional zymotic technology owing to adopted technique scheme, and simple golden flower is provided the solid fermentation process of white tea, guarantees the quality of growing dim of white tea, and fermentation back golden flower bacterium is luxuriant, and golden flower bacterium quantity is greater than 107 in every gram stem tea.This technology improved grow dim quality and quantity, simplified the step of growing dim, shortened the phase of growing dim, and stopped the possibility of the white tea of living contaminants, remedied the potential safety problem of white tea.
The specific embodiment
Below be to implement and further to be described invention, but the present invention do not produced any restriction.
Embodiment 1:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, white tea charging: will handle the white tea that obtains according to the white tea production technology of tradition and place 50 liters of solid-state fermentation tanks, stocking volume is 60% of fermentation tank volume
3, white tea gas steams: steam sterilizing, and be warming up to 90 ℃ and steam 30 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.8% white tea tea juice, adds concentration and be 6% glucose in tea juice, autoclaving
4.2, under aseptic technique, it is 10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
5Individual every milliliter, evenly add the went out Bai Chashang of bacterium of step 3, make the water content control of white tea 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 100 rpm
5, fermentation: keep the water content 100% of tealeaves, the temperature of growing dim is 26 ℃, humidity 86%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 7 days processes of growing dim; Detecting residual glucose content, only is 0.06% of stem tea
6, drying: the white tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea.
Embodiment 2:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, white tea charging: will handle the white tea that obtains according to the white tea production technology of tradition and place 1 ton of solid-state fermentation tank, stocking volume is 80% of fermentation tank volume
3, white tea gas steams: steam sterilizing, and be warming up to 130 ℃ and steam 10 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 10% white tea tea juice, adds concentration and be 20% glucose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
7Individual every milliliter, evenly add the went out Bai Chashang of bacterium of step 3, make the water content control of white tea 20%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 20% of tealeaves, the temperature of growing dim is 31 ℃, humidity 70%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 120 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 5 days processes of growing dim; Detecting residual glucose content, only is 0.7% of stem tea
6, drying: the white tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea.
Embodiment 3:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, white tea charging: will place 100 liters of solid-state fermentation tanks according to the tea dust (particle 100um size) that the white tea production technology of tradition is handled after the white tea that obtains is pulverized through pulverizer, stocking volume is 80% of fermentation tank volume
3, white tea gas steams: steam sterilizing, and be warming up to 110 ℃ and steam 20 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 1% white tea tea juice, adds concentration and be 6% sucrose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
5Individual every milliliter, evenly add the went out Bai Chashang of bacterium of step 3, make the water content control of white tea 80%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 80% of tealeaves, the temperature of growing dim is 30 ℃, humidity 80%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 20 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 6 days processes of growing dim; Detect residual cane sugar content, be 0.8% of stem tea
6, drying: the white tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packaged into tea bag less than 10% in tea, or makes health products.
Embodiment 4:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, white tea charging: will place 500 liters of solid-state fermentation tanks according to the tea dust (particle diameter 5mm size) that the white tea production technology of tradition is handled after the white tea that obtains is pulverized through pulverizer, stocking volume is 60% of fermentation tank volume
3, white tea gas steams: steam sterilizing, and be warming up to 120 ℃ and steam 20 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.8% white tea tea juice, adds concentration and be 6% fructose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
6Individual every milliliter, evenly add the went out Bai Chashang of bacterium of step 3, make the water content control of white tea 40%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 40% of tealeaves, the temperature of growing dim is 28 ℃, humidity 70%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 30 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 7 days processes of growing dim; Detecting residual glucose content, only is 0.3% of stem tea
6, drying: the white tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packaged into tea bag less than 10% in tea, or makes health products.
Claims (8)
1. the solid fermentation process of the white tea of golden flower, it is characterized in that white tea is by the method for the diffusing capsule bacterium of inoculation coronoid process in the process of solid-state fermentation tank fermentation, realized that the pollution-free of white tea grows dim, having prepared length has luxuriant and the white tea of golden flower uniform golden flower bacterium, and will grow dim phase control in 7 days; The white tea of the golden flower that the present invention makes, golden flower bacterium quantity is many, and tealeaves is golden yellow, and golden flower bacterium quantity is greater than 10 in every gram stem tea
7Individual; In white tea, implant the golden flower bacterium first, and utilize solid-state fermentation tank to carry out the making of white tea first, soup coloured gold Huang, fragrance is pure, and flavour is pure and mild, has further improved the quality of white tea; Be conducive to the white tea of the further recognition and acceptance of people.
2. the solid fermentation process of the white tea of telling according to claim 1 of golden flower is characterized in that the white tea of golden flower makes by following operation:
The authentication method of a, the diffusing capsule bacterium of coronoid process, not only will be with colonial morphology, fragrance after the fermentation is compared, more will be by method and diffusing capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence of coronoid process of PCR and order-checking, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude requires could determine more than 99. 5% to be the diffusing capsule bacterium of coronoid process
B, solid-state fermentation tank are aerobic type fermentation tank, require to have the temperature and humidity probe, and the fermentation tank specification is 5 liters ~ 60 tons
C, white tea charging: will handle the white tea that obtains according to the white tea production technology of tradition and place solid-state fermentation tank, stocking volume is 30% ~ 90% of fermentation tank volume
D, white tea gas steam: steam sterilizing, and be warming up to 90 ~ 130 ℃ and steam 10 ~ 30 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
E, inoculation
E.1, inoculation liquid preparation: preparing concentration with pure water is 0.5 ~ 10% white tea tea juice, adds concentration and be 1 ~ 40% glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) in tea juice, autoclaving
E.2, under aseptic technique, it is 1x10 that the inoculation liquid that e.1 prepares with step is adjusted the loose concentration of capsule bacterium of coronoid process
4~ 1x10
7Individual every milliliter, evenly add the went out Bai Chashang of bacterium of steps d, make the water content control of white tea 25 ~ 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
F, fermentation: keep the water content 15 ~ 100% of tealeaves, the temperature of growing dim is 25 ~ 32 ℃, humidity 70 ~ 86%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 ~ 120 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 5 ~ 7 days processes of growing dim; Detect after the fermentation ends glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content, require residual glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content be no more than 1% of dry weight of tea leaves
G, drying: the white tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea, and golden flower bacterium quantity is greater than 10 in every gram stem tea
7Individual.
3. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, it is characterized in that raw material tealeaves both can be to handle the white tea that obtains according to the white tea production technology of tradition, also can be to handle the white tea that obtains through the tea dust (tea dust diameter 100um-1cm) after pulverizing in various degree according to the white tea production technology of tradition.
4. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, solid-state fermentation tank is aerobic type fermentation tank, requires to have the temperature and humidity probe, and the fermentation tank specification is 5 liters ~ 60 tons.
5. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, it is characterized in that white tea steam sterilizing, be warming up to 100 ℃ and steam 20 min that the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process.
6. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, it is characterized in that whole fermentation process will strict sterile working.
7. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, it is characterized in that to detect Escherichia coli and Aspergillus flavus in the finished product.
8. according to claim 1 or 2 solid fermentation process of telling the white tea of golden flower, it is characterized in that the tea dust (tea dust diameter 100um-1cm) of pulverizing the white tea in back in various degree can be packaged into tea bag after fermentation, drying, or make health products.
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| CN110506808A (en) * | 2019-09-29 | 2019-11-29 | 横县南方茶厂 | A kind of processing method of white ginseng bacterium jasmine white tea |
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| CN110800822A (en) * | 2019-11-26 | 2020-02-18 | 普定县久茗茶业有限公司 | Method for preparing white tea |
| CN111449153A (en) * | 2020-03-24 | 2020-07-28 | 茗汲(浙江)生物科技有限公司 | Novel tea beverage and preparation process thereof |
| CN111449153B (en) * | 2020-03-24 | 2023-05-09 | 湖南龙灿生态农业股份有限公司 | Tea beverage and preparation process thereof |
| CN114680208A (en) * | 2022-04-14 | 2022-07-01 | 安吉金甘茶叶科技有限公司 | Fermented white tea and preparation process thereof |
| CN115251203A (en) * | 2022-07-27 | 2022-11-01 | 中华全国供销合作总社济南果品研究所 | Application and method of Xie Washi aspergillus in fermentation preparation of peony petal tea |
| CN115669753A (en) * | 2022-11-17 | 2023-02-03 | 广东济世百草药业有限公司 | Bulk tea fermentation device and solid state fermentation preparation method thereof |
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Application publication date: 20130911 |