CN103283882A - Solid fermentation process of golden flower black tea - Google Patents
Solid fermentation process of golden flower black tea Download PDFInfo
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Abstract
The invention provides a solid fermentation process of golden flower black tea. The solid fermentation process is characterized in that a method for inoculating eurotium cristatum on black tea in a fermentation process of a solid fermentation pot realizes non-pollution blooming of the black tea so as to prepare the golden flower black tea growing with flourish and uniform golden flower bacteria and a blooming flowering phase is controlled within 7 days. The golden flower black tea prepared by the solid fermentation process disclosed by the invention is more in golden flower bacteria quantity; the golden flower bacteria quantity in each gram of dry tea leaves is more than 108; the black tea leaves are yellow; the liquid color is golden yellow, the aroma is pure and the taste is pure and mild; the effect of scraping oil, reducing fat and losing weight is further realized; the solid fermentation pot is firstly used for manufacturing the black tea and a blooming step is simplified; a flowering phase is shortened and the possibility of polluting the black tea by infectious microbes is avoided; the potential safety problem of the black tea is made up; the solid fermentation process is good for people to further approve and accept the black tea.
Description
Technical field
The present invention relates to a kind of black tea, be specially the solid fermentation process of the black tea of golden flower, belong to black tea processing technique field.
Background technology
The golden flower bacterium, namely the diffusing capsule bacterium of coronoid process is found in the Fu brick tea.The Fu brick tea belongs to a most characteristic black tea product of the black tea in the six big teas, and " golden flower " that it wherein has (coronoid process loose capsule bacterium) is that it is exclusive, and its belongs to after fermentation tea, full fermented tea.Modern scientific research, and show by zoopery, human clinical's experiment, toxicologic study, safety evaluatio, epidemiology survey through the authoritative expert: the multiple extraction composition of Fu brick tea " golden flower " all has activation to PPAR γ, PPAR δ, " golden flower " interior two kinds of new active material Fu theine A and Fu theine B have effects such as remarkable reduction human body class fatty compound, blood fat, blood pressure, blood sugar, cholesterol.Long-term drinking Fu tea can promote to regulate metabolism, strengthens human body constitution, delays senility, and human body is played effective pharmacology health care and pathology prevention effect.The special efficacy of Fu tea has obtained domain experts' such as Tea Science, medical science, microbiology extensive approval." would rather not have food on the 3rd, can not no tea on the one ", " no tea on the one then stagnates, no tea on the two then pain, no tea on the three disease then ", the practice of drinking have over the past thousands of years also fully proved this point.
Tens of kinds are contrasted with the different tea sample product drinks that have or not " golden flower " in a collection of tea, find no matter black tea is still deceived tea, more dulcification is sliding sweet refreshing to show as mouthfeel on the more many qualities of golden flower, and the soup look redder is gorgeous bright, can eliminate rapidly in black tea that to produce several years difficult " the heap flavors " that loose in thick blue or green flavor and the fermentation more good, and to digestive system and sleep to improve effect remarkable.The fermentation technique of domestic black tea is confined to traditional pile-fermentation process at present, and golden flower bacterium quantity is on the low side.Expert's health products that extraction of substance is made from the Fu brick tea of Japanology Fu brick tea, price surpasses gold, and saying of " the gold platinum being not as dark fund " therefore arranged.To artificial infection golden flower bacterium, adopt solid fermentation process to produce the black tea of golden flower, increase substantially the report of the quantity of golden flower bacterium and the quality that golden flower deceives tea seldom.
Summary of the invention
The objective of the invention is to be to disclose and a kind ofly can break loaded down with trivial details traditional handicraft method, simple golden flower is provided the solid fermentation process of black tea, guarantee the quality of growing dim of black tea, fermentation back golden flower bacterium is luxuriant, and golden flower bacterium quantity is greater than 10 in every gram stem tea
8Individual.This technology improved grow dim quality and quantity, simplified the step of growing dim, shortened the phase of growing dim, and stopped the possibility of the black tea of living contaminants, remedied the potential safety problem of black tea.
Technical scheme of the present invention is: the solid fermentation process of the black tea of golden flower, by the diffusing capsule bacterium of artificial infection coronoid process, adopt advanced solid-fermented technique to produce the black tea of golden flower.
The solid fermentation process of the black tea of described golden flower is specific as follows:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, similitude requires could determine more than 99.5% to be the diffusing capsule bacterium of coronoid process, could be as bacterial classification
2, black tea charging: will handle the black tea that obtains according to the black tea production technology of tradition and place solid-state fermentation tank, stocking volume is 30% ~ 90% of fermentation tank volume
3, black tea gas steams: steam sterilizing, and be warming up to 90 ~ 130 ℃ and steam 10 ~ 30 min, the solid-state fermentation tank rotating speed is 100-200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.1 ~ 10% black tea tea juice, adds concentration and be 1 ~ 40% glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
4~ 1x10
7Individual every milliliter, evenly add step 3 and went out on the black tea of bacterium, make the water content control of black tea 15 ~ 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 100-200 rpm
5, fermentation: keep the water content 15 ~ 100% of tealeaves, the temperature of growing dim is 25 ~ 32 ℃, humidity 70 ~ 86%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 ~ 120 rpm.Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation.Finish through 5 ~ 7 days processes of growing dim.Detect after the fermentation ends glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content, require residual glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content be no more than 1% of dry weight of tea leaves
6. dry: the black tea of golden flower (pulverizing) with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea.The tea dust (tea dust diameter 100um-1cm) of pulverizing the black tea in back in various degree can be packaged into tea bag after fermentation, drying, or makes health products.
The present invention has broken loaded down with trivial details traditional zymotic technology owing to adopted technique scheme, and simple golden flower is provided the solid fermentation process of black tea, guarantees the quality of growing dim of black tea, and fermentation back golden flower bacterium is luxuriant, and golden flower bacterium quantity is greater than 108 in every gram stem tea.This technology improved grow dim quality and quantity, simplified the step of growing dim, shortened the phase of growing dim, and stopped the possibility of the black tea of living contaminants, remedied the potential safety problem of black tea.
The specific embodiment
Below be to implement and further to be described invention, but the present invention do not produced any restriction.
Embodiment 1:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, black tea charging: will handle the black tea that obtains according to the black tea production technology of tradition and place 50 liters of solid-state fermentation tanks, stocking volume is 60% of fermentation tank volume
3, black tea gas steams: steam sterilizing, and be warming up to 90 ℃ and steam 30 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.8% black tea tea juice, adds concentration and be 6% glucose in tea juice, autoclaving
4.2, under aseptic technique, it is 10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
5Individual every milliliter, evenly add step 3 and went out on the black tea of bacterium, make the water content control of black tea 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 100 rpm
5, fermentation: keep the water content 100% of tealeaves, the temperature of growing dim is 26 ℃, humidity 86%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 7 days processes of growing dim; Detecting residual glucose content, only is 0.06% of stem tea
6, drying: the black tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea.
Embodiment 2:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, black tea charging: will handle the black tea that obtains according to the black tea production technology of tradition and place 1 ton of solid-state fermentation tank, stocking volume is 80% of fermentation tank volume
3, black tea gas steams: steam sterilizing, and be warming up to 130 ℃ and steam 10 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 10% black tea tea juice, adds concentration and be 20% glucose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
7Individual every milliliter, evenly add step 3 and went out on the black tea of bacterium, make the water content control of black tea 20%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 20% of tealeaves, the temperature of growing dim is 31 ℃, humidity 70%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 120 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 5 days processes of growing dim; Detecting residual glucose content, only is 0.7% of stem tea
6, drying: the black tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea.
Embodiment 3:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, black tea charging: will place 100 liters of solid-state fermentation tanks according to the tea dust (particle 100um size) that the black tea production technology of tradition is handled after the black tea that obtains is pulverized through pulverizer, stocking volume is 80% of fermentation tank volume
3, black tea gas steams: steam sterilizing, and be warming up to 110 ℃ and steam 20 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 1% black tea tea juice, adds concentration and be 6% sucrose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
5Individual every milliliter, evenly add step 3 and went out on the black tea of bacterium, make the water content control of black tea 80%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 80% of tealeaves, the temperature of growing dim is 30 ℃, humidity 80%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 20 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 6 days processes of growing dim; Detect residual cane sugar content, be 0.8% of stem tea
6, drying: the black tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packaged into tea bag less than 10% in tea, or makes health products.
Embodiment 4:
1, identify the diffusing capsule bacterium bacterial classification of coronoid process, by colonial morphology, the fragrance after the fermentation is compared; And the method by PCR and order-checking and coronoid process capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence that looses, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, and similitude is 99.9%, as bacterial classification
2, black tea charging: will place 500 liters of solid-state fermentation tanks according to the tea dust (particle diameter 5mm size) that the black tea production technology of tradition is handled after the black tea that obtains is pulverized through pulverizer, stocking volume is 60% of fermentation tank volume
3, black tea gas steams: steam sterilizing, and be warming up to 120 ℃ and steam 20 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
4, inoculation
4.1, inoculation liquid preparation: preparing concentration with pure water is 0.8% black tea tea juice, adds concentration and be 6% fructose in tea juice, autoclaving
4.2, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with step 4.1 is adjusted the loose concentration of capsule bacterium of coronoid process
6Individual every milliliter, evenly add step 3 and went out on the black tea of bacterium, make the water content control of black tea 40%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
5, fermentation: keep the water content 40% of tealeaves, the temperature of growing dim is 28 ℃, humidity 70%, and ventilation from bottom to top, the solid-state fermentation tank rotating speed is 30 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 7 days processes of growing dim; Detecting residual glucose content, only is 0.3% of stem tea
6, drying: the black tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packaged into tea bag less than 10% in tea, or makes health products.
Claims (7)
1. the present invention is the solid fermentation process of the black tea of golden flower, it is characterized in that black tea is by the method for the diffusing capsule bacterium of inoculation coronoid process in the process of solid-state fermentation tank fermentation, realized that the pollution-free of black tea grows dim, having prepared length has luxuriant and the black tea of golden flower uniform golden flower bacterium, and will grow dim phase control in 7 days; The golden flower that the present invention makes deceives tea, and golden flower bacterium quantity is many, and golden flower bacterium quantity is greater than 10 in every gram stem tea
8Individual, make the tealeaves yellowing of black; Soup coloured gold Huang, fragrance is pure, and flavour is pure and mild, has further improved frizing, the effect of lowering blood-fat and reducing weight; And utilize solid-state fermentation tank to deceive the making of tea first, simplified the step of growing dim, shortened the phase of growing dim, and stopped the possibility of the black tea of living contaminants, remedied the potential safety problem of black tea; Be conducive to the black tea of the further recognition and acceptance of people.
2. the golden flower that tells according to claim 1 deceives the solid fermentation process of tea, it is characterized in that the black tea of golden flower makes by following operation:
A, the authentication method of the diffusing capsule bacterium of coronoid process, not only will be with colonial morphology, fragrance after the fermentation is compared, more will be by method and diffusing capsule bacterium 18S rRNA (the Eurotium cristatum gene for 18S rRNA partial sequence of coronoid process of PCR and order-checking, strain:WB 4222) sequence alignment, aligned sequences requires more than the 1500bp, similitude requires could determine 99. 5% or more to be the diffusing capsule bacterium of coronoid process
B, solid-state fermentation tank are aerobic type fermentation tank, and ventilation from bottom to top or axially ventilation require to have the temperature and humidity probe, and the fermentation tank specification is 5 liters ~ 60 tons
Black tea charging: will handle the black tea that obtains according to the black tea production technology of tradition and place solid-state fermentation tank, stocking volume is 30% ~ 90% of fermentation tank volume
C, black tea gas steams: steam sterilizing, be warming up to 90 ~ 130 ℃ and steam 10 ~ 30 min, the solid-state fermentation tank rotating speed is 200 rpm in the sterilization process
D, inoculation liquid preparation: preparing concentration with pure water is 0.5 ~ 10% black tea tea juice, adds concentration and be 1 ~ 40% glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) in tea juice, autoclaving
E, under aseptic technique, it is 1x10 that the inoculation liquid for preparing with steps d is adjusted the loose concentration of capsule bacterium of coronoid process
4~ 1x10
7Individual every milliliter, evenly add step c and went out on the black tea of bacterium, make the water content control of black tea 25 ~ 100%, connect that to keep the solid-state fermentation tank rotating speed in the bacterium process be 200 rpm
F, fermentation: keep the water content 15 ~ 100% of tealeaves, the temperature of growing dim is 25 ~ 32 ℃, humidity 70 ~ 86%, ventilation from bottom to top, the solid-state fermentation tank rotating speed is 10 ~ 120 rpm; Sweat is observed the fermentation situation at any time, adjusts temperature, humidity, rotating speed according to the fermentation situation; Finish through 5 ~ 7 days processes of growing dim
G, detect after the fermentation ends glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content, require residual glucose (also can be fructose, sucrose, soluble starch, lactose, maltose or glycerine) content be no more than 1% of dry weight of tea leaves
H, drying: the black tea of golden flower with fermentation finishes, through sub-sieve, move to the interior negative pressure drying of normal temperature dryer of free from extraneous odour after the compression moulding, moisture can be packed less than 10% in tea, and golden flower bacterium quantity is greater than 10 in every gram stem tea
8Individual.
3. according to claim 1 or 2 solid fermentation process of telling the black tea of golden flower, it is characterized in that raw material tealeaves both can be to handle the black tea that obtains according to the black tea production technology of tradition, also can be to handle the black tea that obtains through the tea dust (tea dust diameter 100um-1cm) after pulverizing in various degree according to the black tea production technology of tradition.
4. according to claim 1 or 2 solid fermentation process of telling the black tea of golden flower, solid-state fermentation tank is aerobic type fermentation tank, requires to have the temperature and humidity probe, and the fermentation tank specification is 5 liters ~ 60 tons.
5. according to claim 1 or 2 solid fermentation process of telling the black tea of golden flower, it is characterized in that whole fermentation process will strict sterile working.
6. according to claim 1 or 2 solid fermentation process of telling the black tea of golden flower, it is characterized in that to detect Escherichia coli and Aspergillus flavus in the finished product.
7. according to claim 1 or 2 solid fermentation process of telling the black tea of golden flower, it is characterized in that the tea dust (tea dust diameter 100um-1cm) of pulverizing the black tea in back in various degree can be packaged into tea bag after fermentation, drying, or make health products.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859081A (en) * | 2014-02-26 | 2014-06-18 | 湖南农业大学 | Aged golden-flower dark green tea and processing technic thereof |
| CN104585383A (en) * | 2015-02-05 | 2015-05-06 | 中南林业科技大学 | Method for producing golden flower dark tea by employing rapid fermentation of raw dark tea brick |
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN106135479A (en) * | 2015-04-14 | 2016-11-23 | 湖南利源隆茶业有限责任公司 | A kind of Fu-brick tea bakes method and Fu-brick tea drying room thereof and prepared Fu-brick tea |
| CN106615313A (en) * | 2016-11-29 | 2017-05-10 | 湖南惟楚福瑞达生物科技有限公司 | Method and device applicable to processing of eurotium cristatum fermented loose tea |
| CN106922905A (en) * | 2017-04-13 | 2017-07-07 | 渤海大学 | A kind of golden flower fermentations dogbane tea and preparation method thereof |
| CN107897407A (en) * | 2017-12-11 | 2018-04-13 | 湖南浩茗茶业食品有限公司 | A kind of method for improving green brick tea quality |
| CN107960491A (en) * | 2017-12-11 | 2018-04-27 | 湖南浩茗茶业食品有限公司 | A kind of green brick tea and preparation method thereof |
| CN109497194A (en) * | 2018-10-29 | 2019-03-22 | 江苏大学 | A kind of fermented tea intelligence process for solid state fermentation |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN103859081A (en) * | 2014-02-26 | 2014-06-18 | 湖南农业大学 | Aged golden-flower dark green tea and processing technic thereof |
| CN103859081B (en) * | 2014-02-26 | 2015-04-22 | 湖南农业大学 | Aged golden-flower dark green tea and processing technic thereof |
| CN104585383A (en) * | 2015-02-05 | 2015-05-06 | 中南林业科技大学 | Method for producing golden flower dark tea by employing rapid fermentation of raw dark tea brick |
| CN106135479A (en) * | 2015-04-14 | 2016-11-23 | 湖南利源隆茶业有限责任公司 | A kind of Fu-brick tea bakes method and Fu-brick tea drying room thereof and prepared Fu-brick tea |
| CN106615313A (en) * | 2016-11-29 | 2017-05-10 | 湖南惟楚福瑞达生物科技有限公司 | Method and device applicable to processing of eurotium cristatum fermented loose tea |
| CN106615313B (en) * | 2016-11-29 | 2023-08-22 | 湖南惟楚福瑞达生物科技有限公司 | Processing device and method suitable for golden flower loose tea |
| CN106922905A (en) * | 2017-04-13 | 2017-07-07 | 渤海大学 | A kind of golden flower fermentations dogbane tea and preparation method thereof |
| CN107897407A (en) * | 2017-12-11 | 2018-04-13 | 湖南浩茗茶业食品有限公司 | A kind of method for improving green brick tea quality |
| CN107960491A (en) * | 2017-12-11 | 2018-04-27 | 湖南浩茗茶业食品有限公司 | A kind of green brick tea and preparation method thereof |
| CN109497194A (en) * | 2018-10-29 | 2019-03-22 | 江苏大学 | A kind of fermented tea intelligence process for solid state fermentation |
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Application publication date: 20130911 |