CN106922905A - A kind of golden flower fermentations dogbane tea and preparation method thereof - Google Patents
A kind of golden flower fermentations dogbane tea and preparation method thereof Download PDFInfo
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- CN106922905A CN106922905A CN201710240254.4A CN201710240254A CN106922905A CN 106922905 A CN106922905 A CN 106922905A CN 201710240254 A CN201710240254 A CN 201710240254A CN 106922905 A CN106922905 A CN 106922905A
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- apocyni veneti
- folium apocyni
- tea
- golden flower
- dogbane
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
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- Tea And Coffee (AREA)
Abstract
The present invention relates to tealeaves manufacture technology field, a kind of golden flower fermentations dogbane tea and preparation method thereof is disclosed, comprised the following steps：Folium Apocyni Veneti is finished, the Folium Apocyni Veneti that water content is 30%~50% is obtained, is carried out after moderate kneads, inoculation golden flower spore suspensions are fermented 6~15 days at 26~28 DEG C；Folium Apocyni Veneti after fermentation dries 30~60min at 60~80 DEG C, obtains dogbane tea.The dogbane tea that the inventive method is prepared can to greatest extent retain the distinctive nutrition and health care composition of bluish dogbane, while the features such as having the color of fermented tea, shape concurrently again, unique flavor is in good taste, is suitable to consumers in general and drinks.
Description
Technical field
The present invention relates to tealeaves manufacture technology field, more particularly to a kind of dogbane tea of fermentation and preparation method thereof.
Background technology
Modern medicine study shows：Folium Apocyni Veneti containing isoquercitrin, element, rutin, several amino acids, organic acid,
Sterol, steroid saponin be former and the material such as triterpenes, the effect of with heat-clearing, soothing the liver, mental-tranquilization, inducing diuresis to remove edema.Can be used for liver
The auxiliary treatment of the illnesss such as positive dizziness, palpitation and insomnia, edema oliguria, high blood pressure, neurasthenia, ephritis edema.
Bluish dogbane fresh leaf is made without fermentation process using traditional handicraft, after being fabricated to tea, there is that leaf color is greener, mesophyll
It is plump, millet paste color is partially green, bitter taste is heavier, poor taste the shortcomings of, had a strong impact on selection of the people to the tea, cause its nothing
Method plays healthcare function.
The content of the invention
In view of this, the present invention provides a kind of in good taste, unique flavor, can preferably retain tealeaves nutritional ingredient and
Fermentation dogbane tea of health-care effect and preparation method thereof.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of preparation method of golden flower fermentation dogbane teas, comprise the following steps：
(1) Folium Apocyni Veneti of harvesting is finished, is obtained the Folium Apocyni Veneti that water content is 30%~50%；
(2) Folium Apocyni Veneti after de-enzyme is kneaded, is kneaded the time for 30~60min；
(3) it is (1~10) × 10 by concentration7The golden flower spore suspensions of cfu/mL in 100~500mL/kg ratio with
It is described knead after Folium Apocyni Veneti mixing be inoculated with, at 26~28 DEG C ferment 6~15 days；
(4) Folium Apocyni Veneti after fermentation is dried into 30~60min at 60~80 DEG C, obtains water content 8%~15%
Dogbane tea.
Wherein, as preferred technical scheme, in step (1), the de-enzyme is microwave de-enzyming, condition：Temperature 100~
150 DEG C, 1~5min of time, 10~20kg of inventory.
Wherein, as preferred technical scheme, in step (3), the golden flower is to isolate and purify to obtain from Fu-brick tea.
Wherein, as preferred technical scheme, in step (4), the drying is dried for microwave drying, temperature 60~80
DEG C, 30~60min of time, 1~5kg of inventory.
Present invention additionally comprises the dogbane tea obtained by above-mentioned preparation method.
The beneficial effects of the invention are as follows：
The present invention is inoculated into Folium Apocyni Veneti using the dominant bacteria-golden flower (i.e. coronoid process dissipate capsule bacterium) in Fu-brick tea process
Middle raised growth breeding, carries out fermented and cultured.The metabolite secreted using golden flower, such as ectoenzyme (such as polyphenol oxidase, pectin
Enzyme, cellulase, protease etc.) effect, realize the conversion of dogbane tea color, quality component, form distinctive hair
Ferment dogbane tea quality and flavor.Of the same race tealeaves of the dogbane tea compared to non-inoculation fermentation fermented through golden flower, soup look, fragrance, taste
There is different degrees of raising in road.After fermentation, soup look more becomes clear, and fragrance is stronger, also add distinctive bacterium fragrant；It is fragrant
Gas composition such as olefines, alkanes, alcohols, ketone, acids, aromatic compound and heterocyclic compound equal size are raised.Through gold
The bitter taste of the dogbane tea after flower fermentation disappears, and taste more enriching alcohol is continuous sliding.Due to the outer enzymatic protein of extracellular microbial and
Tea Polyphenols, makes Tea Polyphenols and caffeine content reduction in the dogbane tea after fermentation, and Tea Pigment total amount is raised；Increased human body must
Must the anti-oxidation active substance such as amino acid classes and EGCG fat, healthcare function enhancing.
Specific embodiment
The invention provides a kind of preparation method of golden flower fermentation dogbane teas, comprise the following steps：
(1) Folium Apocyni Veneti is dried, obtains the Folium Apocyni Veneti that water content is 30%~50%；
(2) dried Folium Apocyni Veneti is kneaded, is kneaded the time for 30~60min；
(3) it is (1~10) × 10 by concentration7The golden flower spore suspensions of cfu/mL in 100~500mL/kg ratio with
It is described knead after Folium Apocyni Veneti mixing be inoculated with, at 26~28 DEG C ferment 6~15 days；
(4) Folium Apocyni Veneti after fermentation is dried into 30~60min at 60~80 DEG C, obtains dogbane tea.
The present invention is to the source of dogbane tea without special restriction, it is preferred to use the Folium Apocyni Veneti of fresh harvesting.In order to
Making the dogbane tea of making has edible safety higher, is dried again after preferably Folium Apocyni Veneti is cleaned.This hair
The bright cleaning way to Folium Apocyni Veneti does not have special restriction, using the traditional cleaning formulations of this area, such as clear water cleaning.
Folium Apocyni Veneti after cleaning is carried out into de-enzyme drying, the moisture on Folium Apocyni Veneti surface is removed, and allows the Folium Apocyni Veneti to carry out part mistake
Water, so as to meet the condition kneaded and ferment.The present invention is 30%~50% by the dried Folium Apocyni Veneti water content of de-enzyme,
Preferred water content is 35%~45%.The present invention does not have special restriction to the Folium Apocyni Veneti dry mode of de-enzyme, it is preferred to use
Microwave carries out de-enzyme drying.The temperature of de-enzyme is 100~150 DEG C, more preferably preferably 120~140 DEG C, 125~135 DEG C.
Inventory when the application is dried to de-enzyme does not have special restriction, when can be dried to de-enzyme according to equipment requirement and actual conditions
Inventory be configured.In the present invention, it is preferred to the inventory that finishes is for 10~20kg, it is further 12~18kg.Above-mentioned
It is preferred that in the case of inventory, the time of microwave de-enzyming is preferably 1~5min, more preferably 2~4min.Finish dried sieve
Cloth sesame slices meet the requirement kneaded.The dried Folium Apocyni Veneti of finishing is kneaded, further the tissue of destruction tealeaves or
Histocyte, is easy to it fully to ferment.In the present invention, the time kneaded be 30~60min, more preferably 36~
54min, more preferably 42~49min.Degree is kneaded to be kneaded using the moderate of this area.The present invention is to the equipment kneaded without spy
Different restriction, is kneaded using the equipment in the conventional tealeaves manufacture craft of this area, such as kneading machine.The process of kneading can be by
Folium Apocyni Veneti cytosol fractions are twisted with the fingers and rub leaf surfaces, are contacted beneficial to golden flower (coronoid process dissipate capsule bacterium), promote the carrying out of fermentation.
The present invention with golden flower to kneading after Folium Apocyni Veneti ferment.The present invention is to the source of golden flower without special limit
It is fixed.Golden flower used is that the golden flower for obtaining is isolated and purified from Fu-brick tea in embodiments of the present invention.Isolate and purify and use ability
The usual manner in domain.In an embodiment of the present invention, the isolation and purification method can be specially：Aseptically, use
Tealeaves fragment of the aseptic inoculation pin picking with coronoid process dissipate capsule bacterium yellow cleistothecium is cultivated in accessing PDA agar mediums,
Cultivation temperature is 26~30 DEG C, preferably 27~28 DEG C.The bacterium colony after culture is purified after 3~5d.According on PDA plate
The colony characteristicses of growth carry out primary dcreening operation, and line separation is carried out with the different single bacterium colony of aseptic inoculation ring picking, single until obtaining
Strain.Single culture single-point is inoculated in czapek agar medium, constant incubator is inverted in and is cultivated, cultivation temperature is 26
~30 DEG C, more preferably preferably 27~29 DEG C, 28 DEG C.The daily observed and recorded since incubated the 7th day, control
《Chinese fungi will》Tentatively judged, and collection material send microorganism fungus kind appraisal company to be identified, in obtaining Fu-brick tea
The golden flower for isolating and purifying.
In the present invention, the golden flower of inoculation is preferably golden flower spore suspensions.Make golden flower spore suspensions process be
The conventional preparation method of this area, specific method is：The coronoid process dissipate capsule bacterium obtained in Fu-brick tea is isolated and purified, with connecing after sterilizing
Kind of ring gently scrapes golden yellow mycelium on flat board in sterilized water, and is centrifuged after adding the small bead of sterilizing, after filtering i.e.
Obtain golden flower spore suspensions.In the present invention, the rotating speed of the centrifugation is preferably 1000~3000r/min, more preferably
1500~2500r/min；Centrifugation time is preferably 3~5min, is specifically 4min.The present invention is using sterile absorbent cotton to centrifugation
Liquid afterwards is filtered, but is not limited to this kind of filter type.Golden flower spore suspensions are obtained after filtering.Adjustment spore suspension
Liquid concentration to required concentration is used for inoculation fermentation.
In the present invention, the concentration for being inoculated with golden flower spore suspensions is (1~10) × 107Cfu/mL, preferably (2~8) ×
107Cfu/mL, more preferably (3~7) × 107cfu/mL.Golden flower spore suspensions are uniformly sprayed on Folium Apocyni Veneti,
Be sufficiently mixed contacting with the Folium Apocyni Veneti after kneading.The present invention does not have special restriction to the mode for mixing, and can reach
The effect that golden flower is well mixed with Folium Apocyni Veneti is within protection scope of the present invention.In the present invention, golden flower spores hang
The mixed proportion of supernatant liquid and Folium Apocyni Veneti for 100~500mL/kg, preferably 200~400mL/kg, more preferably 250~
350mL/kg。
Postvaccinal Folium Apocyni Veneti is carried out into ferment at constant temperature, fermentation temperature is 26~28 DEG C, preferably 27 DEG C；Fermentation time
It is 6~15 days, preferably 8~13 days, more preferably 10~12 days.Dogbane tea surface after fermentation covers with golden flower and is
Fermentation is completed.
Dogbane tea after fermentation is into tea after drying.Drying temperature is 60~80 DEG C, preferably 63~76 DEG C, is entered
One step is preferably 66~73 DEG C.The present invention is not particularly limited to dry mode, using this area conventional drying mode.
Drying drying, 60~80 DEG C of temperature, more preferably 70~75 are preferably carried out using microwave in the specific embodiment of the invention
℃；Microwave power is preferably 200~400W, more preferably 300~350W.Fed intake when the application is dried to microwave drying
Without special restriction, inventory when can be dried to microwave drying according to equipment requirement and actual conditions is configured amount.
In the present invention, the inventory that preferably finishes is 1~5kg, is further 2~4kg.In the case of above-mentioned preferred inventory, drying
Time is preferably 30~60min, more preferably more preferably 35~54min, 42~49min.Golden flower hairs are obtained after drying
The moisture of ferment dogbane tea is 8%~15%, preferably 10%~13%, and as bluish dogbane is into tea.
Present invention additionally comprises the golden flower fermentation dogbane teas produced using the above method.
With reference to embodiment, the present invention will be described in detail, but they can not be interpreted as protecting the present invention
The restriction of scope.
Embodiment 1
A kind of golden flower fermentations dogbane tea, its preparation method is comprised the following steps：
(1) the Folium Apocyni Veneti 20kg that will be plucked microwave drying 1min at 150 DEG C, obtain the bluish dogbane that water content is 35%
Leaf；
(2) dried Folium Apocyni Veneti is carried out into moderate in kneading machine and kneads 30min, by Folium Apocyni Veneti cytosol fractions
Sth. made by twisting rubs leaf surfaces, is contacted beneficial to coronoid process dissipate capsule bacterium, promotes fermentation to carry out；
(3) it is 1 × 10 by concentration7The golden flower spore suspensions of cfu/mL are sprayed on after kneading in the ratio of 500mL/kg
Folium Apocyni Veneti surface, is sufficiently mixed and is inoculated with, and is fermented 15 days at 26 DEG C；Folium Apocyni Veneti surface produces " golden flower ", has fermented
Into；
(4) by the Folium Apocyni Veneti 5kg after fermentation at 60 DEG C microwave drying 56min, microwave power is 400W, obtains aqueous
The bluish dogbane for 10% is measured into tea.
Embodiment 2
A kind of golden flower fermentations dogbane tea, its preparation method is comprised the following steps：
(1) the Folium Apocyni Veneti 10kg that will be plucked microwave drying 3min at 130 DEG C, obtain the bluish dogbane that water content is 40%
Leaf；
(2) dried Folium Apocyni Veneti is carried out into moderate in kneading machine and kneads 43min, by Folium Apocyni Veneti cytosol fractions
Sth. made by twisting rubs leaf surfaces, is contacted beneficial to coronoid process dissipate capsule bacterium, promotes fermentation to carry out；
(3) it is 5 × 10 by concentration7The golden flower spore suspensions of cfu/mL are sprayed on after kneading in the ratio of 200mL/kg
Folium Apocyni Veneti surface, is sufficiently mixed and is inoculated with, and is fermented 7 days at 28 DEG C；Folium Apocyni Veneti surface produces " golden flower ", has fermented
Into；
(4) by the Folium Apocyni Veneti 3kg after fermentation at 72 DEG C microwave drying 48min, microwave power is 300W, obtains aqueous
The bluish dogbane for 12% is measured into tea.
Embodiment 3
A kind of golden flower fermentations dogbane tea, its preparation method is comprised the following steps：
(1) the Folium Apocyni Veneti 13kg that will be plucked microwave drying 5min at 100 DEG C, obtain the bluish dogbane that water content is 45%
Leaf；
(2) dried Folium Apocyni Veneti is carried out into moderate in kneading machine and kneads 56min, by Folium Apocyni Veneti cytosol fractions
Sth. made by twisting rubs leaf surfaces, is contacted beneficial to coronoid process dissipate capsule bacterium, promotes fermentation to carry out；
(3) it is 10 × 10 by concentration7The golden flower spore suspensions of cfu/mL are sprayed on after kneading in the ratio of 100mL/kg
Folium Apocyni Veneti surface, be sufficiently mixed and be inoculated with, at 27 DEG C ferment 12 days；Folium Apocyni Veneti surface produces " golden flower ", fermentation
Complete；
(4) by the Folium Apocyni Veneti 2kg after fermentation at 80 DEG C microwave drying 32min, microwave power is 200W, obtains aqueous
The bluish dogbane for 14% is measured into tea.
The golden flower fermentation dogbane teas produced to embodiment 1~3 carry out sensory evaluation, the results are shown in Table 1.
The sensory evaluation of the golden flower fermentation dogbane teas that the embodiment of the present invention 1~3 of table 1 is produced
The golden flower fermentation dogbane tea component contents produced to embodiment 1~3 by the following method are measured, as a result
It is shown in Table 2.
Tea Polyphenols is determined：Ferrous tartrate colorimetric method；Determined amino acid：Ninhydrin colorimetry；Caffeine is determined：Ultraviolet point
Light photometry；Total sugar determination：Anthrone~Sulphuric acid colorimetry；Theaflavin, thearubigin and theabrownin are determined：System analysis method；Table does not have
Infanticide catechin gallic acid fat is determined：High performance liquid chromatography；Analysis of aroma components：Gas-chromatography~mass spectrography.
The golden flower fermentation dogbane tea component contents that the embodiment of the present invention 1~3 of table 2 is produced are determined
As can be seen from Table 2, the reduction of Tea Polyphenols, caffeine and amino acid content, Tea Pigment in the dogbane tea after fermentation
Total amount is raised；The anti-oxidation active substance such as fragrance component and EGCG fat is increased, healthcare function increases
By force.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (5)
1. a kind of preparation method of golden flower fermentations dogbane tea, comprises the following steps：
(1) Folium Apocyni Veneti is finished, is obtained the Folium Apocyni Veneti that water content is 30%~50%；
(2) Folium Apocyni Veneti after the de-enzyme is kneaded, is kneaded the time for 30~60min；
(3) it is (1~10) × 10 by concentration7The golden flower spore suspensions of cfu/mL are rubbed in the ratio of 100~500mL/kg with described
Folium Apocyni Veneti mixing after sth. made by twisting is inoculated with, and is fermented 6~15 days at 26~28 DEG C；
(4) Folium Apocyni Veneti after the fermentation is dried into 30~60min at 60~80 DEG C, obtains water content 8%~15%
Dogbane tea.
2. preparation method according to claim 1, it is characterised in that in step (1), the de-enzyme is microwave de-enzyming, temperature
100~150 DEG C of degree, 1~5min of time, 10~20kg of inventory.
3. preparation method according to claim 1, it is characterised in that in step (3), the golden flower is from Fu-brick tea point
Obtained from purifying.
4. preparation method according to claim 1, it is characterised in that in step (4), the drying is dry for microwave drying
It is dry, 60~80 DEG C of temperature, 30~60min of time, 1~5kg of inventory.
5. the dogbane tea that the method according to Claims 1 to 4 any one is prepared.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710240254.4A CN106922905A (en) | 2017-04-13 | 2017-04-13 | A kind of golden flower fermentations dogbane tea and preparation method thereof |
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| CN201710240254.4A CN106922905A (en) | 2017-04-13 | 2017-04-13 | A kind of golden flower fermentations dogbane tea and preparation method thereof |
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| CN101731395A (en) * | 2008-11-20 | 2010-06-16 | 高殿学 | Method for producing apocynum venetum tea |
| CN103283882A (en) * | 2012-03-05 | 2013-09-11 | 刘立辉 | Solid fermentation process of golden flower black tea |
| CN103283881A (en) * | 2012-03-05 | 2013-09-11 | 刘立辉 | Solid fermentation process of golden flower white tea |
| CN104431176A (en) * | 2014-12-09 | 2015-03-25 | 北京龙程菁华生物科技有限公司 | Fermented tea of pepper leaves and jinhua fungus and preparation method and application of fermented tea |
| CN105010667A (en) * | 2015-07-05 | 2015-11-04 | 新疆阿勒泰戈宝麻有限公司 | Sleep aiding apocynum venetum tea and preparation method thereof |
| CN106306185A (en) * | 2016-08-25 | 2017-01-11 | 福建省农业科学院茶叶研究所 | Golden camellia white tea and preparation method thereof |
| CN106417724A (en) * | 2016-06-24 | 2017-02-22 | 中国土产畜产进出口总公司 | Loose tea Eurotium cristatum growth technology |
-
2017
- 2017-04-13 CN CN201710240254.4A patent/CN106922905A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731395A (en) * | 2008-11-20 | 2010-06-16 | 高殿学 | Method for producing apocynum venetum tea |
| CN103283882A (en) * | 2012-03-05 | 2013-09-11 | 刘立辉 | Solid fermentation process of golden flower black tea |
| CN103283881A (en) * | 2012-03-05 | 2013-09-11 | 刘立辉 | Solid fermentation process of golden flower white tea |
| CN104431176A (en) * | 2014-12-09 | 2015-03-25 | 北京龙程菁华生物科技有限公司 | Fermented tea of pepper leaves and jinhua fungus and preparation method and application of fermented tea |
| CN105010667A (en) * | 2015-07-05 | 2015-11-04 | 新疆阿勒泰戈宝麻有限公司 | Sleep aiding apocynum venetum tea and preparation method thereof |
| CN106417724A (en) * | 2016-06-24 | 2017-02-22 | 中国土产畜产进出口总公司 | Loose tea Eurotium cristatum growth technology |
| CN106306185A (en) * | 2016-08-25 | 2017-01-11 | 福建省农业科学院茶叶研究所 | Golden camellia white tea and preparation method thereof |
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Application publication date: 20170707 |