CN102084903B - Preparation process of fungus-grown loosened green tea - Google Patents
Preparation process of fungus-grown loosened green tea Download PDFInfo
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- CN102084903B CN102084903B CN 201010598193 CN201010598193A CN102084903B CN 102084903 B CN102084903 B CN 102084903B CN 201010598193 CN201010598193 CN 201010598193 CN 201010598193 A CN201010598193 A CN 201010598193A CN 102084903 B CN102084903 B CN 102084903B
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Abstract
The invention provides a preparation process of fungus-grown loosened green tea, which comprises the steps of: 1, purifying and separating eurotium cristatum; 2, preparing an eurotium cristatum spore suspension and determining the concentration thereof; 3, processing a green tea sample; 4, adding the eurotium cristatum suspension; 5, drying the tea, 6, controlling the humidity and temperature for fungus growing; 7, ageing and drying the fungus-grown loosened green tea; and 8, storing the fungus-grown loosened green tea. The invention can realize the fungus growing of the green tea by inoculating by using the golden flower fungus in Fuzhuan tea in a short period, and golden flower is generally flourish, bright and yellow in color, and large in particles, thus the fungus-grown loosened green tea inherits the impressions of the traditional green tea and can be endowed with the characteristics of the Fuzhuan tea, and the internal quality and the additional value of the green tea are improved.
Description
Technical field:
The present invention relates to technical field of agricultural product process, be specifically related to traditional green tea is carried out after fermentation processing and makes the grow dim preparation technology of loose tea of green tea.Described flower is the loose capsule bacterium of coronoid process, is commonly called as golden flower.
Background technology:
Green tea claims again azymic tea, is take suitable And Development of Tea Shoot as raw material, the tealeaves of making through typical process processes such as completing, knead, be dry.Take green as homophony, therefore named at the bottom of millet paste after it is done dark brown pool and brews, the leaf.The characteristic of green tea, more reservation the natural materials in the bright leaf.Wherein the Tea Polyphenols caffeine keeps more than 85% of bright leaf, and chlorophyll keeps about 50%, and vitamin loss is also less, thereby has formed the characteristics of green tea " clear soup greenery, the flavour convergence is strong ".In the Chinese green tea, famous-object is maximum, and not only fragrant high flavor is long, excellent quality, and moulding is unique, have higher value of art appreciation, green tea is different by its drying and fixing method, generally is divided into fried green, baking green grass or young crops, shines green grass or young crops and steaming green tea, but literature search shows that traditional green tea is carried out after fermentation improves its interior quality research or blank, traditional green tea is given the flavor characteristics of its Fu brick tea, not only can be increased the added value of green tea, and promoted the interior quality of green tea, black tea.
Tea namely is called as " medicines of ten thousand diseases " at ancient times.Especially to the present age, the factors such as fat surplus, radiation, environmental pollution and pressure multiplier cause many modern diseases such as hypertension, high fat of blood, coronary heart disease, diabetes, hypercholesterolemia, cancer and mental handicape etc., and the Fu brick tea has certain curative effect and prevention effect to this.Modern scientific research shows, the Fu brick tea to the pharmacological function of human body mainly be because tealeaves contain the number of chemical composition and found at present hundreds of trace elements and microorganism arranged.Particularly, the loose capsule bacterium of exclusive coronoid process in the Fu brick tea, after people drank tea, these active ingredients were also absorbed by human body thereupon, thereby brought a lot of benefits for people's health.By animal experiment, the human clinical trial proves conclusively the Fu brick tea and have equally significant lowering blood-fat and reducing weight and function of reducing blood sugar in experimental animal and human body, and strict toxicological assessment shows, it is safe that long-term high dose is drunk Fu tea.The epidemiology survey that carry out the Fu brick tea emphasis consumption areas such as western part of China Xinjiang, Inner Mongol, Qinghai shows, long-term drinking Fu tea has good regulating action to blood fat, blood sugar, the blood pressure of human body, body weight, the bodily form are had good regulating and controlling effect, and can significantly strengthen human body constitution, delay senility.The Fu brick tea was the indispensable important beverage of borderland ethnic group in the past, and existing also coming into vogue interiorly drunk the Fu brick tea, but because its raw material---Dark Green Tea is comparatively thick old, and particularly fluorinated volume is higher, adds the characteristics on the impression, and interior ground acceptance level is popularized far away.
Summary of the invention:
Technical problem to be solved by this invention is: solve the problem that above-mentioned prior art exists, and provide a kind of preparation process of fungus-grown loosened green tea, realize that with the inoculation of the golden flower bacterium in the Fu brick tea green tea grows dim in a short time, and golden flower generally luxuriant, lovely luster is golden yellow, particle is loose, the impression characteristics of traditional green tea had both been inherited, can give again its Fu brick tea characteristics, promote its inherent quality and added value.
The technical solution used in the present invention is: this preparation process of fungus-grown loosened green tea the steps include:
1) purifies and separates of the loose capsule bacterium of coronoid process;
2) the eurotium cristatum spore suspension preparation is determined with concentration;
3) processing of green tea sample;
4) add the loose capsule bacteria suspension of coronoid process;
5) tea curing;
6) controlled humidity and temperature are grown dim;
7) green tea ageing, the drying of loose tea of growing dim;
8) the green tea preservation of loose tea of growing dim.
In the technique scheme, the purifies and separates of the loose capsule bacterium of described coronoid process is: separate with the PDA Solid agar culture culture purified of adding 10% tea juice;
The eurotium cristatum spore suspension preparation prepares with the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl; Spore suspension concentration is determined 10
7-10
8Individual/mL;
The processing controls humidity of green tea sample is about 28%;
Golden flower suspension addition is 0.5mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour;
Controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, 26 ℃ of temperature, and then humidity 70%, and 28 ℃ of temperature are until golden flower grows generally luxuriantly, bright in colour golden yellow, and particle is loose, till arohid flavour is strong; Then humidity drops to 60% gradually, temperature is increased to 36 ℃, green tea ageing 7 days gradually; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
PDA refers to that the abbreviation Chinese implication of Potato Dextrose Agar is: potato dextrose agar.
The PDA Solid agar culture refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, slightly cold rear with the double gauze filtration, in filtrate, add 15g agar, heat fused, add at last sucrose 20g, mixing is settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).
The PDA fluid nutrient medium refers to by above-mentioned culture medium process for preparation operation, but does not add agar.
The PDA flat board refers to be cooled to about 50 ℃ after the sterilization of above-mentioned PDA Solid agar culture finishes, on superclean bench, culture medium poured into sterilized in advance and dry culture dish in, it is to be cooled that to be the PDA Solid agar culture that makes dull and stereotyped.
Improvement PDA solid medium refers to potato is cleaned peeling, takes by weighing 200g, is cut into small pieces and puts into beaker, add an amount of distilled water, boiled 1 hour, slightly cold filter with double gauze afterwards, in filtrate, add the tea juice that 100mL prepares, then add 15g agar, heat fused adds sucrose 20g at last, mixing, be settled to 1000mL with graduated cylinder, be divided in while hot in the triangular flask, at 1.1kg/cm
2Keep sterilization in 20 minutes in (121 ℃).Be cooled to about 50 ℃ after sterilization finishes, on superclean bench, the PDA Solid agar culture of 10% tea juice poured into sterilized in advance and dry culture dish in, the PDA Solid agar culture that is the 10% tea juice that makes to be cooled is dull and stereotyped.
Improvement PDA fluid nutrient medium refers to by above-mentioned culture medium process for preparation operation, but does not add agar.
Dull and stereotyped gradient dilution method is: finished product Fu brick tea sample is pulverized sample with the plant micro pulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10mL namely is made into 1 * 10
-1The Fu brick sample solution of g/mL is got 1mL constant volume 10mL and is made into 1 * 10 from mentioned solution
-2The Fu brick sample solution of g/mL is mixed with concentration 1 * 10 according to this
-3, 1 * 10
-4, 1 * 10
-5Each 10mL of Fu brick sample solution of g/mL is for subsequent use.Get 1 * 10
-3, 1 * 10
-4, 1 * 10
-5Each 0.5mL of Fu brick sample solution of g/mL is applied on the quick isolation medium flat board of crown loose capsule bacterium with spreader, dries up on the super-clean bench about 15 minutes, is inverted plate, 28 ℃ of cultivations, and picking dominant bacteria after 3-4 days---golden flower similarity condition is cultivated until purifying.
The setting-out inoculation refers to separate corresponding microorganism with the bacterium colony picking that it is good that the transfer needle of sterilization will be grown in new solid medium plate setting-out in super-clean bench.
The golden flower bacterium colony refers to that the golden flower bacterium grows into a bacterium colony aggregate that forms certain the time at the 10%PDA culture medium flat plate, falls to referring to the microbic mass that flocks together.
The preparation method of tea juice is: take by weighing 20g Fu brick tea according to 1g Fu brick tea sample than the ratio of 30mL distilled water, add 600mL distilled water, 100 ℃ of water-baths 2 hours are settled to 600mL with graduated cylinder and are the tea juice for preparing after slightly cooling off.
Be made into 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Refer to concentration, unit is g/mL.
Spore concentration at outstanding night 10
7-10
8" individual " among individual/m l refers to the ascospore of single golden flower bacterium.Method of counting is counted or with its OD value of 722 type spectrophotometer measurements, is controlled at about 0.6-0.8 for being coated on the gradient dilution method to grow on the PDA Solid agar culture flat board that adds 10% tea juice, makes golden flower spore suspension concentration be controlled at every milliliter 10
7-10
8Individual golden flower spore, above-mentioned OD value is light absorption value.
The present invention in a short time (3-4 days) realizes that with the inoculation of the golden flower bacterium in the Fu brick tea green tea grows dim, and golden flower is generally luxuriant, and is bright in colour golden yellow, and particle is loose, both inherited the impression characteristics of traditional green tea, and can give again its Fu brick tea characteristics, promote interior quality and added value.For traditional green tea carries out after fermentation processing, makes the green tea loose tea that grows dim a kind of new New Techniques is provided.
Description of drawings:
Fig. 1 is the loose capsule bacterium colony photo of the coronoid process of separation and purification of the present invention after 7 days;
Fig. 2 is that eurotium cristatum spore suspension of the present invention is cultivated 7 days as a result photos;
Fig. 3 is the brand 1 green tea as a result photo of loose tea that grows dim;
Fig. 4 is the brand 2 green tea as a result photos of loose tea that grow dim.
The specific embodiment
1, the separation of golden flower, purifying:
The capsule bacterium (being golden flower) that falls apart separates from the benefit board Fu brick sample of Yiyang tea processing factory finished product Hunan coronoid process.Concrete separate and purge process is: finished product Hunan benefit board Fu brick sample is pulverized, with dull and stereotyped gradient dilution method on the PDA flat board of interpolation 10% tea juice 28 ℃ of cultivations until purifying.
2, the preparation of the spore suspension of golden flower is determined with concentration:
The golden flower bacterium colony of picking purifying is seeded to and adds in the improvement PDA fluid nutrient medium, cultivates 6-7 days, and makes spore suspension concentration be controlled at 10 for 28 ℃
7-10
8Individual/mL.
3, the processing of green tea sample:
Steam 10 minutes softening green tea with high-temperature steam, controlled humidity is 28% simultaneously.
4, add the golden flower spore suspension:
Add the ratio of 0.5mL golden flower spore suspension according to every 100g tea, add with the form of spraying, fully mixing.
5, tea curing:
65-68 ℃ in confined conditions of tea behind interpolation golden flower spore suspension and the mixing, baking 1 hour.
6, controlled humidity and temperature are grown dim:
Controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then humidity 70%, 28 ℃ of temperature until golden flower grow generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong.
7, the green tea ageing of loose tea of growing dim:
Humidity drops to 60% gradually, temperature is increased to 36 ℃, green tea ageing 7 days gradually.8 loose teas drying and preservation:
Loose tea 40-50 ℃ drying is after 1 day, the indoor preservation of normal temperature.
Embodiment 1: the separation of golden flower, purifying:
Finished product Hunan benefit board Fu brick tea sample is pulverized with the plant micro pulverizer, and 200 orders sieve, 65 ℃ of dryings take by weighing the 1g sample after 2 hours, and constant volume 10ml is made into concentration 10
-1, 10
-2, 10
-3, 10
-4, 10
-5Each 10mL gets 10
-3, 10
-4, 10
-5Three each 0.0.5mL of gradient coat the PDA flat board that adds 10% tea juice, dry up on the super-clean bench, and be inverted 28 ℃ and cultivated 3 days, the golden yellow dominant colony of picking, setting-out is to the PDA flat board that adds 10% tea juice, and similarity condition continues to cultivate until purifying.
The golden flower bacterium colony of separation and purification is seen accompanying drawing 1.
Embodiment 2: the spore suspension preparation of golden flower:
The golden flower bacterium colony of picking purifying, be seeded in the PDA fluid nutrient medium that adds 10% tea juice, 20% sucrose, 10%NaCl, 28 ℃ leave standstill cultivation 2 days, then the temperature that raises was to 30-32 ℃ of cultivation 7-10 days,, then the 280rpm shaken cultivation is disperseed conidium, filters with double gauze, recording the OD value with 722 type spectrophotometers is 0.6-0.8, is available golden flower spore suspension.
The spore suspension cultivation results of golden flower is seen accompanying drawing 2.
Embodiment 3: the checking that the green tea loose tea grows dim:
Get respectively two kinds of each 1Kg of green tea of commercially available different brands, carry out loose tea according to the 2-8 step in the specific embodiment and ferment.
The green tea loose tea grows dim and the results are shown in accompanying drawing 3, Fig. 4.
Claims (1)
1. preparation process of fungus-grown loosened green tea is characterized in that step is:
1) purifies and separates of the loose capsule bacterium of coronoid process;
2) the eurotium cristatum spore suspension preparation is determined with concentration; The coronoid process of the picking purifying capsule bacterium bacterium colony that falls apart is seeded to and adds in the improvement PDA fluid nutrient medium, cultivates 6-7 days, and makes spore suspension concentration be controlled at 10 for 28 ℃
7-10
8Individual/mL; Described improvement PDA fluid nutrient medium is for adding the PDA fluid nutrient medium of 10% tea juice, 20% sucrose, 10%NaCl;
3) processing of green tea sample;
4) add eurotium cristatum spore suspension; Add the ratio of 0.5ml eurotium cristatum spore suspension according to every 100g tea, add with the form of spraying, fully mixing;
5) tea curing; 65-68 ℃ in confined conditions of tea behind interpolation eurotium cristatum spore suspension and the mixing, baking 1 hour;
6) controlled humidity and temperature are grown dim; Controlled humidity and the temperature condition of growing dim is 26 ℃ of a few days ago humidity 80%, temperature, then humidity 70%, 28 ℃ of temperature until the loose capsule bacteria growing of coronoid process generally luxuriant, lovely luster is golden yellow, particle is loose, till arohid flavour is strong;
7) green tea ageing, the drying of loose tea of growing dim;
8) the green tea preservation of loose tea of growing dim.
2, preparation process of fungus-grown loosened green tea according to claim 1 is characterized in that: the purifies and separates of the loose capsule bacterium of described coronoid process is: separate with the PDA Solid agar culture culture purified of adding 10% tea juice.
3, preparation process of fungus-grown loosened green tea according to claim 1 is characterized in that: the processing controls humidity of green tea sample is 28%.
4, preparation process of fungus-grown loosened green tea according to claim 1 is characterized in that: the eurotium cristatum spore suspension addition is 0. 5mL/100g; The tea curing condition is airtight 65-68 ℃, 1 hour.
5, preparation process of fungus-grown loosened green tea according to claim 1, it is characterized in that: controlled humidity and the temperature condition of growing dim are: a few days ago humidity 80%, 26 ℃ of temperature, then humidity 70%, 28 ℃ of temperature are until the loose capsule bacteria growing of coronoid process is generally luxuriant, bright in colour golden yellow, particle is loose, till arohid flavour is strong; Then humidity drops to 60% gradually, temperature is increased to 36 ℃, green tea ageing 7 days gradually; Loose tea 40-50 ℃ drying is the indoor preservation of normal temperature after 1 day.
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| CN102640814B (en) * | 2012-05-14 | 2014-02-12 | 咸阳泾渭茯茶有限公司 | Method for making golden flower loose tea without inoculation |
| CN102860389B (en) * | 2012-05-23 | 2015-01-07 | 华中农业大学 | Lotus leaf eurotium cristatum tea and preparation method thereof |
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| CN104543056A (en) * | 2014-12-31 | 2015-04-29 | 四川省文君茶业有限公司 | Fuzhuan tea processing method with green tea used as blank |
| CN105420020B (en) * | 2015-12-15 | 2018-07-06 | 贵州大学 | A kind of method using coronoid process dissipate capsule bacterium production tea wine |
| CN105851299A (en) * | 2016-04-21 | 2016-08-17 | 贵州梵锦茶业有限公司 | Method for preparing black brick tea from black tea |
| CN109645159A (en) * | 2017-10-11 | 2019-04-19 | 南京农业大学 | A method of utilizing the black loose tea of the high-quality floating of southern Jiangsu finished product Green Tea Processing |
| CN108029829B (en) * | 2017-12-29 | 2021-05-11 | 中南林业科技大学 | Preparation method of paper mulberry golden camellia |
| CN109997930A (en) * | 2019-04-15 | 2019-07-12 | 益阳冠隆誉黑茶发展有限公司 | Tealeaves nature fungus growing process, tea Processing method and equipment under non-compaction |
| CN110178929A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of processing method of Pilochun (a green tea) Fu tea floating |
| CN110178934A (en) * | 2019-05-08 | 2019-08-30 | 西咸新区茯茶镇茶业有限公司 | A kind of Longjing tea Fu tea floating and preparation method thereof |
| CN113142341B (en) * | 2021-02-04 | 2022-04-01 | 广东省农业科学院茶叶研究所 | Making process of dried orange peel golden flower green tea with blood sugar reducing effect |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579024A (en) * | 2009-06-18 | 2009-11-18 | 谌小丰 | Artificial inoculation production method of camellia nitidissima |
| CN101669559A (en) * | 2008-09-12 | 2010-03-17 | 广西民族大学 | Production method for improving quality of Liupu tea |
-
2010
- 2010-12-21 CN CN 201010598193 patent/CN102084903B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101669559A (en) * | 2008-09-12 | 2010-03-17 | 广西民族大学 | Production method for improving quality of Liupu tea |
| CN101579024A (en) * | 2009-06-18 | 2009-11-18 | 谌小丰 | Artificial inoculation production method of camellia nitidissima |
Non-Patent Citations (2)
| Title |
|---|
| 徐瑞瑞,等.绿茶液冠突散囊菌发酵期间品质变化的研究.《安徽农业大学学报》.2010,第37卷(第3期), * |
| 魏晓惠.金花菌不同接种量固态发酵对绿毛茶品质的影响.《贵州农业科学》.2010,第38卷(第6期), * |
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