CN104630112A - Acinetobacter as well as preparation method and application of acinetobacter - Google Patents

Acinetobacter as well as preparation method and application of acinetobacter Download PDF

Info

Publication number
CN104630112A
CN104630112A CN201510067447.5A CN201510067447A CN104630112A CN 104630112 A CN104630112 A CN 104630112A CN 201510067447 A CN201510067447 A CN 201510067447A CN 104630112 A CN104630112 A CN 104630112A
Authority
CN
China
Prior art keywords
acinetobacter calcoaceticus
strain
preparation
terephthalic acid
flat board
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510067447.5A
Other languages
Chinese (zh)
Other versions
CN104630112B (en
Inventor
张翼
任烨
王海云
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHAOXING KEQIAO JIANGBIN WATER TREATMENT Co Ltd
Original Assignee
SHAOXING KEQIAO JIANGBIN WATER TREATMENT Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHAOXING KEQIAO JIANGBIN WATER TREATMENT Co Ltd filed Critical SHAOXING KEQIAO JIANGBIN WATER TREATMENT Co Ltd
Priority to CN201510067447.5A priority Critical patent/CN104630112B/en
Publication of CN104630112A publication Critical patent/CN104630112A/en
Application granted granted Critical
Publication of CN104630112B publication Critical patent/CN104630112B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/34—Organic compounds containing oxygen

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Hydrology & Water Resources (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to acinetobacter which is classified to be Acinetobacter sp.T1 and is collected with the number of China center for type culture collection (CCTCC) NO.M2014558 in CCTCC on November 9th, 2014. The acinetobacter is applied to degradation of terephthalic acid in printing and dyeing wastewater. The acinetobacter disclosed by the invention is better in degradation performance for terephthalic acid in the printing and dyeing wastewater, higher in application value, high in degradation efficiency, short in required time, better in degradation effect under an aerobic condition and wider in application range.

Description

One strain acinetobacter calcoaceticus and preparation method thereof, application
Technical field
The present invention relates to strain acinetobacter calcoaceticus and preparation method thereof, application, the application of the terephthalic acid especially in degrading waste water, belongs to technical field of microbe application.
Background technology
Alkali decrement waste water results from the alkali deweighting technique of polyester piece good, the main component of alkali decrement waste water is (as quaternary ammonium salt cationic surface-active agent, alkali-resistant penetrant and N to dioctyl phthalate (PTA), ethylene glycol (EG), polyester oligomer and a small amount of auxiliary agent, N-polyoxyethylene pheynylalkylamine) etc., wherein, PTA accounts for 63% ~ 71% of the total lotus of COD, is the characteristic pollutent [1] of alkali decrement waste water.In alkali decrement waste water, PTA is with " three wastes " form entered environment, and in some waste water (as PTA factory effluent and polyester production waste water), the concentration of PTA is up to thousands of milligrams per liter [2].PTA has stimulation toxic [3] to fish, has the effect of suppression to the regeneration of microorganism in water, has teratogenesis and mutagenesis [4-5] to some animal.Effectively process the PTA in alkali decrement waste water, be the problem that people pay close attention to always.
The main method of current treatment PTA waste water is: the materialization treatment process of charcoal absorption, ozone or Chlorine Dioxide Oxidation and the biochemical treatment process etc. with aerobic, anaerobism or anaerobic-aerobic. the biological degradation treatment technology of PTA is paid close attention to widely in recent years, and Chinese scholars is to the large quantifier elimination [6-10] that how to have utilized microbiological treatment PTA factory effluent to carry out.Numerous research shows that PTA can be decomposed by multiple-microorganism and can be degraded as the only carbon source of microorganism. and the microorganism of degraded PTA is extensively present in soil, river, the active sludge of sewage work and compost. and single bacterial strain, mixed bacterium and active sludge can be degraded PTA under aerobic and anaerobic condition, and aerobic degradation speed is more faster than anaerobic degradation speed.In current existing bibliographical information, the bacterium that someone screens the PTA that can degrade comprises Gram-negative and positive bacillus, sarcina, silk bacterium and yeast.The people such as Chen Peng find a kind of candiyeast, can to the PTA degraded more than 80% of 1400mg/L in 72 hours, and degradation efficiency is better, but degradation rate is slower.
Therefore, for solving the problems of the technologies described above, the necessary application providing a kind of new strain acinetobacter calcoaceticus and degraded terephthalic acid thereof, to overcome described defect of the prior art.
Summary of the invention
For solving the problems of the technologies described above, the first object of the present invention is to provide a kind of strain acinetobacter calcoaceticus being easy to obtain, degraded terephthalic acid is effective and good to dyeing waste water adaptability.
The second object of the present invention is the application providing a strain acinetobacter calcoaceticus.
The third object of the present invention is the preparation method providing a strain acinetobacter calcoaceticus.
For realizing above-mentioned first object, the technical scheme that the present invention takes is: a strain acinetobacter calcoaceticus, its this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558.
For realizing above-mentioned second object, the technical scheme that the present invention takes is: the application of a strain acinetobacter calcoaceticus, and it is for the terephthalic acid in high-level efficiency degraded dyeing waste water.
The application of a strain acinetobacter calcoaceticus of the present invention is further: its inoculum size is 1%, and temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), and shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
For realizing above-mentioned 3rd object, the technical scheme that the present invention takes is: the preparation method of a strain acinetobacter calcoaceticus, and it comprises following processing step:
1), enrichment: get aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out P-phthalic acid at concentration and to decline more bacterial strain, make glycerine pipe and preserve.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: in step 1), comprises following composition (unit is g/L): yeast extract powder 5.0, peptone 10.0, NaCl10.0 in enrichment medium, adjusts pH to 7.0 with 1mol/L NaOH.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: in step 1), and the amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: step 2) in, comprise following composition (unit is g/L) in screening culture medium: NH 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
The preparation method of a strain acinetobacter calcoaceticus of the present invention also can be: in step 4), comprises following composition (unit is g/L): NH in degraded substratum 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
Compared with prior art, the present invention has following beneficial effect:
1. a strain acinetobacter calcoaceticus degraded terephthalic acid of the present invention is effective, and required time is short; Can degrade the terephthalic acid of higher concentration, range of application is wider.
2. a strain acinetobacter calcoaceticus screening of the present invention is in the aeration tank active sludge of process dyeing and printing sewage, has better adaptability, more have using value to dyeing and printing sewage.
Embodiment
The present invention is a strain acinetobacter calcoaceticus, this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558, preservation address: China, Wuhan, Wuhan University.
Above-mentioned acinetobacter calcoaceticus has following feature: bacterium colony is white, circular, neat in edge, and smooth surface is moistening.Basis of microscopic observation is shaft-like, without gemma, and atrichia, Gram-negative.
An above-mentioned strain acinetobacter calcoaceticus can be used for specific pollutants terephthalic acid in degraded dyeing waste water, wherein a kind of degradation method is: its inoculum size is 1%, temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
An above-mentioned strain acinetobacter calcoaceticus can be adopted and obtain with the following method:
1), enrichment: Zhong Qu aeration tank, aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times; Wherein, comprise following composition (unit is g/L) in enrichment medium: yeast extract powder 5.0, peptone 10.0, NaCl10.0, adjust pH to 7.0 with 1mol/L NaOH; The amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium; Wherein, following composition (unit is g/L) is comprised in screening culture medium: NH 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out P-phthalic acid at concentration and to decline more bacterial strain, make glycerine pipe and preserve; Wherein, following composition (unit is g/L) is comprised in degraded substratum: NH 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
Above embodiment is only the preferred embodiment of this creation, and not in order to limit this creation, any amendment made within all spirit in this creation and principle, equivalent replacement, improvement etc., within the protection domain that all should be included in this creation.

Claims (8)

1. a strain acinetobacter calcoaceticus, is characterized in that: this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558.
2. the application of a strain acinetobacter calcoaceticus as claimed in claim 1, is characterized in that: for the terephthalic acid in high-level efficiency degraded dyeing waste water.
3. the application of a strain acinetobacter calcoaceticus as claimed in claim 2, it is characterized in that: its inoculum size is 1%, temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
4. the preparation method of a strain acinetobacter calcoaceticus, is characterized in that: comprise following processing step:
1), enrichment: get aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out the bacterial strain that P-phthalic acid at concentration declines by a big margin, make glycerine pipe and preserve.
5. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, it is characterized in that: in step 1), comprise following composition (unit is g/L) in enrichment medium: yeast extract powder 5.0, peptone 10.0, NaCl10.0, adjusts pH to 7.0 with 1mol/L NaOH.
6. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: in step 1), and the amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100.
7. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: step 2) in, comprise following composition (unit is g/L) in screening culture medium: NH 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
8. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: in step 4), comprises following composition (unit is g/L): NH in degraded substratum 4cl1.0, KH 2pO 43.0, Na 2hPO 47.0, NaCl0.5, MgSO 47H 2o0.25, PTA(terephthalic acid) 1.0, adjust pH to 7.5 with 1mol/L NaOH.
CN201510067447.5A 2015-02-10 2015-02-10 One plant of acinetobacter calcoaceticus and preparation method thereof, application Expired - Fee Related CN104630112B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510067447.5A CN104630112B (en) 2015-02-10 2015-02-10 One plant of acinetobacter calcoaceticus and preparation method thereof, application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510067447.5A CN104630112B (en) 2015-02-10 2015-02-10 One plant of acinetobacter calcoaceticus and preparation method thereof, application

Publications (2)

Publication Number Publication Date
CN104630112A true CN104630112A (en) 2015-05-20
CN104630112B CN104630112B (en) 2017-09-29

Family

ID=53209364

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510067447.5A Expired - Fee Related CN104630112B (en) 2015-02-10 2015-02-10 One plant of acinetobacter calcoaceticus and preparation method thereof, application

Country Status (1)

Country Link
CN (1) CN104630112B (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106916773A (en) * 2017-05-10 2017-07-04 南京工业大学 One plant degraded diethyl terephthalate bacterial strain and its application
CN108359617A (en) * 2017-12-29 2018-08-03 浙江双良商达环保有限公司 Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization
CN108774625A (en) * 2017-12-29 2018-11-09 浙江双良商达环保有限公司 Acinetobacter calcoaceticus CL04 and its application in the processing of villages and small towns sewage dephosphorization
CN109576170A (en) * 2018-08-30 2019-04-05 常州大学 One plant height imitates application of the sulfur oxidizing bacterium in Containing Sulfur Black wastewater treatment
CN110283745A (en) * 2019-06-27 2019-09-27 浙江工业大学 Hospital acinetobacter calcoaceticus FK2 and its application in degradable organic pollutant

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
C.VAMSEE-KRISHNA ET AL.: "Biodegradation of Phthalate Isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and Acinetobacter lwoffii ISP4", 《APPLIED MICROBIAL AND CELL PHYSIOLOGY》 *
冯杨阳: "一株对苯二甲酸降解菌的鉴定及其降解特性", 《化工学报》 *
田静，李尔炀: "高效处理化纤废水", 《江苏工业学院学报》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106916773A (en) * 2017-05-10 2017-07-04 南京工业大学 One plant degraded diethyl terephthalate bacterial strain and its application
CN106916773B (en) * 2017-05-10 2019-04-12 南京工业大学 One plant degradation diethyl terephthalate bacterial strain and its application
CN108359617A (en) * 2017-12-29 2018-08-03 浙江双良商达环保有限公司 Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization
CN108774625A (en) * 2017-12-29 2018-11-09 浙江双良商达环保有限公司 Acinetobacter calcoaceticus CL04 and its application in the processing of villages and small towns sewage dephosphorization
CN108359617B (en) * 2017-12-29 2021-03-02 浙江双良商达环保有限公司 Acinetobacter CL05 and application thereof in village and town sewage dephosphorization treatment
CN109576170A (en) * 2018-08-30 2019-04-05 常州大学 One plant height imitates application of the sulfur oxidizing bacterium in Containing Sulfur Black wastewater treatment
CN110283745A (en) * 2019-06-27 2019-09-27 浙江工业大学 Hospital acinetobacter calcoaceticus FK2 and its application in degradable organic pollutant
CN110283745B (en) * 2019-06-27 2021-05-11 浙江工业大学 Acinetobacter hospital FK2 and application thereof in degrading organic pollutants

Also Published As

Publication number Publication date
CN104630112B (en) 2017-09-29

Similar Documents

Publication Publication Date Title
CN104630112A (en) Acinetobacter as well as preparation method and application of acinetobacter
CN103045579B (en) Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same
CN103589661B (en) Gemmobacter aquaticus as well as application thereof
CN105274025B (en) A kind of composite flora and its treatment process of heavy metal-polluted water process
CN106635858A (en) Paracoccus sp. and culture application thereof
CN110791444B (en) Pseudomonas stutzeri, composite microbial inoculum prepared from pseudomonas stutzeri and application of composite microbial inoculum
CN105132323A (en) Salt-tolerance bacillus and application thereof in high-salinity wastewater treatment
CN104276735A (en) Environment-friendly technology used for purifying electroplating sewage
CN105062936A (en) Compound halophilous-microorganism bacterium agent and application thereof
CN106635855B (en) Microbacterium and its culture application are seen in a kind of north
CN103381418B (en) Method for processing tobacco waste or organic fluorine wastewater
CN108102943B (en) Denitrifying microorganism and application thereof
CN106399200B (en) Alcaligenes and application thereof in high-salt high-polymer wastewater
CN112608857B (en) Salt-tolerant bacillus pumilus SZG-NY-001 capable of repairing polluted water and application thereof
CN115261372A (en) Solid microbial inoculum for repairing electroplating polluted site and method and application thereof
CN111004749B (en) Salt-tolerant bacillus lentus GBW-HB1902 and application thereof
CN111378597B (en) Manganese oxidizing bacterium capable of being used for demanganization and application thereof
CN107487868A (en) A kind of method that nano cupric oxide cooperates with Cr VI in water removal with bacterium
CN109182210B (en) Bacterial strain for sewage treatment
CN104560746A (en) Candida tropicalis capable of treating high-salinity organic chemical wastewater, and application of candida tropicalis
CN107446912B (en) Immobilized saccharomyces cerevisiae engineering bacteria and method for removing heavy metal Pb in wastewater by using same2+In (1)
CN102108334B (en) Chromium-removing rhodobacterium and application thereof
CN106635856B (en) The open cultural method of Pseudomonas stutzeri FSTB-5 a kind of and its application
CN106630190B (en) Fungus agent for degrading COD and preparation method thereof
CN105861375B (en) A kind of microphenomenon of degradation of aniline and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170929

Termination date: 20220210