CN104630112A - Acinetobacter as well as preparation method and application of acinetobacter - Google Patents
Acinetobacter as well as preparation method and application of acinetobacter Download PDFInfo
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- CN104630112A CN104630112A CN201510067447.5A CN201510067447A CN104630112A CN 104630112 A CN104630112 A CN 104630112A CN 201510067447 A CN201510067447 A CN 201510067447A CN 104630112 A CN104630112 A CN 104630112A
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- acinetobacter calcoaceticus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
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Abstract
The invention relates to acinetobacter which is classified to be Acinetobacter sp.T1 and is collected with the number of China center for type culture collection (CCTCC) NO.M2014558 in CCTCC on November 9th, 2014. The acinetobacter is applied to degradation of terephthalic acid in printing and dyeing wastewater. The acinetobacter disclosed by the invention is better in degradation performance for terephthalic acid in the printing and dyeing wastewater, higher in application value, high in degradation efficiency, short in required time, better in degradation effect under an aerobic condition and wider in application range.
Description
Technical field
The present invention relates to strain acinetobacter calcoaceticus and preparation method thereof, application, the application of the terephthalic acid especially in degrading waste water, belongs to technical field of microbe application.
Background technology
Alkali decrement waste water results from the alkali deweighting technique of polyester piece good, the main component of alkali decrement waste water is (as quaternary ammonium salt cationic surface-active agent, alkali-resistant penetrant and N to dioctyl phthalate (PTA), ethylene glycol (EG), polyester oligomer and a small amount of auxiliary agent, N-polyoxyethylene pheynylalkylamine) etc., wherein, PTA accounts for 63% ~ 71% of the total lotus of COD, is the characteristic pollutent [1] of alkali decrement waste water.In alkali decrement waste water, PTA is with " three wastes " form entered environment, and in some waste water (as PTA factory effluent and polyester production waste water), the concentration of PTA is up to thousands of milligrams per liter [2].PTA has stimulation toxic [3] to fish, has the effect of suppression to the regeneration of microorganism in water, has teratogenesis and mutagenesis [4-5] to some animal.Effectively process the PTA in alkali decrement waste water, be the problem that people pay close attention to always.
The main method of current treatment PTA waste water is: the materialization treatment process of charcoal absorption, ozone or Chlorine Dioxide Oxidation and the biochemical treatment process etc. with aerobic, anaerobism or anaerobic-aerobic. the biological degradation treatment technology of PTA is paid close attention to widely in recent years, and Chinese scholars is to the large quantifier elimination [6-10] that how to have utilized microbiological treatment PTA factory effluent to carry out.Numerous research shows that PTA can be decomposed by multiple-microorganism and can be degraded as the only carbon source of microorganism. and the microorganism of degraded PTA is extensively present in soil, river, the active sludge of sewage work and compost. and single bacterial strain, mixed bacterium and active sludge can be degraded PTA under aerobic and anaerobic condition, and aerobic degradation speed is more faster than anaerobic degradation speed.In current existing bibliographical information, the bacterium that someone screens the PTA that can degrade comprises Gram-negative and positive bacillus, sarcina, silk bacterium and yeast.The people such as Chen Peng find a kind of candiyeast, can to the PTA degraded more than 80% of 1400mg/L in 72 hours, and degradation efficiency is better, but degradation rate is slower.
Therefore, for solving the problems of the technologies described above, the necessary application providing a kind of new strain acinetobacter calcoaceticus and degraded terephthalic acid thereof, to overcome described defect of the prior art.
Summary of the invention
For solving the problems of the technologies described above, the first object of the present invention is to provide a kind of strain acinetobacter calcoaceticus being easy to obtain, degraded terephthalic acid is effective and good to dyeing waste water adaptability.
The second object of the present invention is the application providing a strain acinetobacter calcoaceticus.
The third object of the present invention is the preparation method providing a strain acinetobacter calcoaceticus.
For realizing above-mentioned first object, the technical scheme that the present invention takes is: a strain acinetobacter calcoaceticus, its this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558.
For realizing above-mentioned second object, the technical scheme that the present invention takes is: the application of a strain acinetobacter calcoaceticus, and it is for the terephthalic acid in high-level efficiency degraded dyeing waste water.
The application of a strain acinetobacter calcoaceticus of the present invention is further: its inoculum size is 1%, and temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), and shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
For realizing above-mentioned 3rd object, the technical scheme that the present invention takes is: the preparation method of a strain acinetobacter calcoaceticus, and it comprises following processing step:
1), enrichment: get aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out P-phthalic acid at concentration and to decline more bacterial strain, make glycerine pipe and preserve.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: in step 1), comprises following composition (unit is g/L): yeast extract powder 5.0, peptone 10.0, NaCl10.0 in enrichment medium, adjusts pH to 7.0 with 1mol/L NaOH.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: in step 1), and the amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100.
The preparation method of a strain acinetobacter calcoaceticus of the present invention is further: step 2) in, comprise following composition (unit is g/L) in screening culture medium: NH
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
The preparation method of a strain acinetobacter calcoaceticus of the present invention also can be: in step 4), comprises following composition (unit is g/L): NH in degraded substratum
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
Compared with prior art, the present invention has following beneficial effect:
1. a strain acinetobacter calcoaceticus degraded terephthalic acid of the present invention is effective, and required time is short; Can degrade the terephthalic acid of higher concentration, range of application is wider.
2. a strain acinetobacter calcoaceticus screening of the present invention is in the aeration tank active sludge of process dyeing and printing sewage, has better adaptability, more have using value to dyeing and printing sewage.
Embodiment
The present invention is a strain acinetobacter calcoaceticus, this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558, preservation address: China, Wuhan, Wuhan University.
Above-mentioned acinetobacter calcoaceticus has following feature: bacterium colony is white, circular, neat in edge, and smooth surface is moistening.Basis of microscopic observation is shaft-like, without gemma, and atrichia, Gram-negative.
An above-mentioned strain acinetobacter calcoaceticus can be used for specific pollutants terephthalic acid in degraded dyeing waste water, wherein a kind of degradation method is: its inoculum size is 1%, temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
An above-mentioned strain acinetobacter calcoaceticus can be adopted and obtain with the following method:
1), enrichment: Zhong Qu aeration tank, aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times; Wherein, comprise following composition (unit is g/L) in enrichment medium: yeast extract powder 5.0, peptone 10.0, NaCl10.0, adjust pH to 7.0 with 1mol/L NaOH; The amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium; Wherein, following composition (unit is g/L) is comprised in screening culture medium: NH
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out P-phthalic acid at concentration and to decline more bacterial strain, make glycerine pipe and preserve; Wherein, following composition (unit is g/L) is comprised in degraded substratum: NH
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
Above embodiment is only the preferred embodiment of this creation, and not in order to limit this creation, any amendment made within all spirit in this creation and principle, equivalent replacement, improvement etc., within the protection domain that all should be included in this creation.
Claims (8)
1. a strain acinetobacter calcoaceticus, is characterized in that: this strain bacterium classification acinetobacter calcoaceticus T1(Acinetobacter sp.T1 by name), be preserved in China typical culture collection center (CCTCC) on November 9th, 2014, preserving number is CCTCC NO. M 2014558.
2. the application of a strain acinetobacter calcoaceticus as claimed in claim 1, is characterized in that: for the terephthalic acid in high-level efficiency degraded dyeing waste water.
3. the application of a strain acinetobacter calcoaceticus as claimed in claim 2, it is characterized in that: its inoculum size is 1%, temperature is 20-40 DEG C, pH is 6-8, dyeing waste water (P-phthalic acid at concentration is 100-1000mg/L), shaking speed is detect its degradation efficiency after 150-200r/min, 24h.
4. the preparation method of a strain acinetobacter calcoaceticus, is characterized in that: comprise following processing step:
1), enrichment: get aeration tank water, and add in enrichment medium, stir under the speed of 180r/min, cultivate 1 day, get pregnant solution after 1 day, and add in enrichment medium for 30 DEG C, so repeat 2 times;
2), screening: get pregnant solution and carry out dilution painting flat board, and cultivate 1-2 days, until flat board grows bacterium colony with 30 DEG C in screening culture medium;
3), separation and purification and Molecular Identification: the bacterium colony that flat board grows is carried out line purifying on beef extract-peptone flat board, cultivate 24-48h, choose the single bacterium colony grown and repeat line 3 times for 30 DEG C; Single bacterium colony is trained bacterium liquid and makes the preservation of glycerine pipe;
4), multiple sieve: preparation degraded substratum, the bacterial strain of inoculation purifying, inoculum size is 1%, cultivates the concentration that 24 as a child detected terephthalic acid in degraded substratum, picks out the bacterial strain that P-phthalic acid at concentration declines by a big margin, make glycerine pipe and preserve.
5. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, it is characterized in that: in step 1), comprise following composition (unit is g/L) in enrichment medium: yeast extract powder 5.0, peptone 10.0, NaCl10.0, adjusts pH to 7.0 with 1mol/L NaOH.
6. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: in step 1), and the amount of water and enrichment medium is 1:100; The amount of pregnant solution and enrichment medium is 1:100.
7. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: step 2) in, comprise following composition (unit is g/L) in screening culture medium: NH
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, agar 15, adjusts pH to 7.5 with 1mol/L NaOH.
8. the preparation method of a strain acinetobacter calcoaceticus as claimed in claim 4, is characterized in that: in step 4), comprises following composition (unit is g/L): NH in degraded substratum
4cl1.0, KH
2pO
43.0, Na
2hPO
47.0, NaCl0.5, MgSO
47H
2o0.25, PTA(terephthalic acid) 1.0, adjust pH to 7.5 with 1mol/L NaOH.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106916773A (en) * | 2017-05-10 | 2017-07-04 | 南京工业大学 | Strain for degrading diethyl terephthalate and application thereof |
CN108359617A (en) * | 2017-12-29 | 2018-08-03 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization |
CN108774625A (en) * | 2017-12-29 | 2018-11-09 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL04 and its application in the processing of villages and small towns sewage dephosphorization |
CN109576170A (en) * | 2018-08-30 | 2019-04-05 | 常州大学 | One plant height imitates application of the sulfur oxidizing bacterium in Containing Sulfur Black wastewater treatment |
CN110283745A (en) * | 2019-06-27 | 2019-09-27 | 浙江工业大学 | Hospital acinetobacter calcoaceticus FK2 and its application in degradable organic pollutant |
-
2015
- 2015-02-10 CN CN201510067447.5A patent/CN104630112B/en not_active Expired - Fee Related
Non-Patent Citations (3)
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C.VAMSEE-KRISHNA ET AL.: "Biodegradation of Phthalate Isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and Acinetobacter lwoffii ISP4", 《APPLIED MICROBIAL AND CELL PHYSIOLOGY》 * |
冯杨阳: "一株对苯二甲酸降解菌的鉴定及其降解特性", 《化工学报》 * |
田静,李尔炀: "高效处理化纤废水", 《江苏工业学院学报》 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106916773A (en) * | 2017-05-10 | 2017-07-04 | 南京工业大学 | Strain for degrading diethyl terephthalate and application thereof |
CN106916773B (en) * | 2017-05-10 | 2019-04-12 | 南京工业大学 | Strain for degrading diethyl terephthalate and application thereof |
CN108359617A (en) * | 2017-12-29 | 2018-08-03 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL05 and its application in the processing of villages and small towns sewage dephosphorization |
CN108774625A (en) * | 2017-12-29 | 2018-11-09 | 浙江双良商达环保有限公司 | Acinetobacter calcoaceticus CL04 and its application in the processing of villages and small towns sewage dephosphorization |
CN108359617B (en) * | 2017-12-29 | 2021-03-02 | 浙江双良商达环保有限公司 | Acinetobacter CL05 and application thereof in village and town sewage dephosphorization treatment |
CN109576170A (en) * | 2018-08-30 | 2019-04-05 | 常州大学 | One plant height imitates application of the sulfur oxidizing bacterium in Containing Sulfur Black wastewater treatment |
CN110283745A (en) * | 2019-06-27 | 2019-09-27 | 浙江工业大学 | Hospital acinetobacter calcoaceticus FK2 and its application in degradable organic pollutant |
CN110283745B (en) * | 2019-06-27 | 2021-05-11 | 浙江工业大学 | Acinetobacter hospital FK2 and application thereof in degrading organic pollutants |
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