CN106916773B - One plant degradation diethyl terephthalate bacterial strain and its application - Google Patents
One plant degradation diethyl terephthalate bacterial strain and its application Download PDFInfo
- Publication number
- CN106916773B CN106916773B CN201710328523.2A CN201710328523A CN106916773B CN 106916773 B CN106916773 B CN 106916773B CN 201710328523 A CN201710328523 A CN 201710328523A CN 106916773 B CN106916773 B CN 106916773B
- Authority
- CN
- China
- Prior art keywords
- diethyl terephthalate
- bacterial strain
- degradation
- diethyl
- terephthalate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses the bacterial strains of one plant of diethyl terephthalate of degrading, its classification naming is Delftiatsuruhatensis (Delftia sp.), bacterial strain number is WL-3, it has been preserved in China typical culture collection center, deposit number are as follows: CCTCC NO:M 2017136, the deposit date is on March 21st, 2017.The invention also discloses the applications of above-mentioned bacterial strains diethyl terephthalate in degradation soil.Bacterial strain WL-3 is aerobic microorganism, and optimum growth temperature is 30 DEG C, and the most suitable growth pH is 7.0.Bacterial strain WL-3 can be with the diethyl terephthalate of degradable 1000mg/L in 7 days, and utilizes it as sole carbon source growth, can its degradable metabolite terephthalic acid (TPA).The present invention has important application value for the degradation of diethyl terephthalate and to its metabolite on the biological treating of industrial wastewater.
Description
Technical field
The invention belongs to biologic treating technique fields, are related to the bacterial strain of a high-efficiency degradation diethyl terephthalate and answer
With.
Background technique
First with the fast development and extensive use of polyester plastics (PET) industry, it is useless that a large amount of PET can be all generated every year
Gurry.There are about the generations of the polyester waste material of 400kt every year in China, will not be directly right although these polyester waste materials toxicity itself is little
Environment causes damages, but due to its waste huge amount, and is difficult to degrade under field conditions (factors), causes greatly white
Pollution.Since PET has hydrophobicity and high-crystallinity, directly to set about screening microorganism or the enzyme of energy efficient degradation from PET
It is extremely difficult.
So the degradation of PET is tamed as structural simulation object and is cultivated in this experiment using diethyl terephthalate (DET)
Bacterium.Firstly, diethyl terephthalate is a kind of Environmental Hormone as industrial chemicals itself, serious dirt can be generated to environment
Dye.Secondly, diethyl terephthalate will release terephthalic acid (TPA) (TPA) under the action of microorganism and hydrolase, it is one
The very important industrial chemicals of kind, is widely used in the industries such as synthetic resin, polyester fiber, plastic film and dyestuff, but to water
The regeneration of middle microorganism has inhibiting effect, has the prominent property of cause and carcinogenesis to animal, is acknowledged as a kind of dirt of toxic property
Contaminate object.The biodegrade research of terephthalic acid (TPA) is more at present, but the treatment process of diethyl terephthalate is still far from perfect.
Zhang Jianfei et al. isolates 5 plants of purebred bacterium to the plastc ring for having degradation, and is screened out from it to degrade to DTP and make
With best bacterial strain F4, the diethyl terephthalate for the 5000ppm that degrades, the degradation rate after 14 days can be more than 92%.Study benzene
The biodegradation of dicarboxylate not only can solve pollution problem of the industrial chemicals to environment itself, can also be
The biodegrade research of PET lays a good foundation.
Summary of the invention
The technical problem to be solved by the present invention is in PET biodegrade diethyl terephthalate and industrial wastewater
The biological treating of terephthalic acid (TPA) provides a kind of microorganism of efficient degradation diethyl terephthalate.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
The bacterial strain of one plant of degradation diethyl terephthalate, classification naming are Delftiatsuruhatensis (Delftia sp.), bacterium
Strain number is WL-3, has been preserved in China typical culture collection center (abbreviation CCTCC), and deposit number is CCTCC NO:M
2017136, the deposit date is on March 21st, 2017, preservation address are as follows: the Chinese Wuhan Wuhan University.The bacterial strain is inventor
It is screened in November, 2016.
Delftia WL-3 screening technique of the present invention: using diethyl terephthalate as the inorganic of sole carbon source
Salt is supported in base (pH 7.0), is degraded to the diethyl terephthalate (DET) in the industrial wastewater containing ramification of benzene
Bacterial strain carries out separation screening.
Specifically, using the industrial wastewater containing ramification of benzene as screening target, using containing 1000mg/L to benzene
The minimal medium of dicarboxylate has diethyl terephthalate Utilization ability as medium, continuous acclimating
Bacterial strain, by minimal medium dilution spread in the inorganic salts solid medium containing 1000mg/L diethyl terephthalate,
30 DEG C of culture 2-3d, the different diethyl terephthalate degradation bacteria strains of picking form carry out scribing line separation and obtain pure culture, life
Entitled bacterial strain WL-3 verifies purebred microbial strains WL-3 to diethyl terephthalate degradation capability again, utilizes efficient liquid
Phase chromatography detects diethyl terephthalate residual content；
Specifically, the minimal medium is by ammonium nitrate 1.0g, sodium chloride 1.0g, potassium dihydrogen phosphate 0.5g, phosphorus
Sour hydrogen dipotassium 1.5g adds water to 1.0L preparation, adjusts pH value to 7.0, agar 20.0g, 121 DEG C of sterilizings is added in solid medium
15min uses the diethyl terephthalate of preceding addition final concentration 1000mg/L as carbon source.
WL-3 bacterial strain is after 30 DEG C of incubator cultures for 24 hours, bacterium colony milky, in protuberance, circle, opaque shape.WL-3 bacterium
Strain Starch Hydrolysis reaction is negative, contact that enzyme reaction is positive, urase reaction is positive, V-P reacting positive, clark and Lubsreaction are positive
Property, oxydase reaction is positive, gelatin liquefaction reacting positive.The Physiology and biochemistry qualification result of bacterial strain WL-3 and the spy of Delftiatsuruhatensis
It levies closest.
Specifically, the 1/3LB culture medium is added water to by peptone 3.0g, yeast powder 1.5g, sodium chloride 3.0g
1.0L is prepared, and adjusts pH value to 7.0, agar 20.0g, 121 DEG C of sterilizing 15min is added in solid medium.
The nucleotide sequence of bacterial strain WL-3 of the present invention, 16S rDNA are as shown in SEQ ID NO:1 in sequence table.
One kind containing diethyl terephthalate of the present invention and its metabolite degradation bacteria strains Delftia WL-3
The cloning vector of 16S rDNA sequence.
The recombinant cloning vector, the carrier that preferably sets out are pMD19T.
Containing the diethyl terephthalate and its metabolite degradation bacteria strains Deftia WL-3 16S rDNA sequence
Genetic engineering bacterium Escherich coli DH5 α (pMD19T-16S).
The genetic engineering bacterium Escherich coli DH5 α construction method: primer 2 7F:5`- is utilized
The 16S rDNA of AGAGTTTGATCCTGGCTCAG-3` and 1492R:5`-TACCTTGTTACGACTT-3` amplification bacterial strain WL-3,
Cloning vector pMD19T is connected in such a way that T/A is cloned, constructs recombinant cloning vector pMD19T-16S, is transformed into gram
Grand host strain Escherich coli DH5 α obtains recombinant microorganism Escherich coli DH5 α (pMD19T-16S), by institute
The recombinant microorganism exogenous sequences of acquisition are sequenced, and ncbi database compares the 16S rDNA sequence, on a molecular scale will
Bacterial strain WL-3 is identified to Delftiatsuruhatensis category.
Application of the above-mentioned bacterial strains WL-3 in degrading waste water in diethyl terephthalate is also in protection scope of the present invention
Within.
Wherein, concentration preferences of the diethyl terephthalate in waste water are 250mg/L~1000mg/L.
Application of one plant of diethyl terephthalate degradation bacteria strains Delftia WL-3 in wastewater treatment process, it is specific to walk
It is rapid as follows:
(1) bacterial strain WL-3 seed liquor culture: bacterial strain WL-3 seed liquor uses LB liquid medium, takes test tube slant preservation
Bacterial strain WL-3 chooses bacterium colony with oese and accesses the triangular flask containing 100mL LB liquid medium, and 30 DEG C of shaken cultivations 12 are small
When, shaking speed 200rmin-1, OD is made after minimal medium washes twice in the thallus of acquisition600nm=1.0 bacterium
Suspension is as Degrading experiment seed liquor；
(2) dynamics of bacterial strain WL-3 degradation diethyl terephthalate: final concentration of in diethyl terephthalate
In the minimal medium of 1000mg/L, seed liquor, in 30 DEG C, 180rmin are accessed by 5% inoculum concentration-1Shake culture, every
1d sampling is primary, measures OD600nmWith the residual concentration of diethyl terephthalate.
(3) influence of temperature and pH to bacterial strain WL-3 degradation diethyl terephthalate: at diethyl terephthalate end
Concentration is seed liquor to be accessed by 5% inoculum concentration, respectively at 20 DEG C, 25 DEG C, 30 DEG C, 37 in the minimal medium of 1000mg/L
DEG C, 42 DEG C, pH 7.0,180rmin-1Shake culture；Respectively at initial pH 4.0,6.0,6.5., 7.0,7.5,8,9,10,30
℃、180r·min-1The influence of shake culture, measuring temperature and pH to bacterial strain WL-3 degradation diethyl terephthalate.
(4) influence of initial substrate concentration and inoculum concentration to bacterial strain WL-3 degradation diethyl terephthalate: to benzene two
In the minimal medium of the final concentration of 1000mg/L of formic acid diethylester, accessed respectively by 1%, 3%, 5%, 10% inoculum concentration
Seed liquor is in 30 DEG C, 180rmin-1Shake culture；Respectively in diethyl terephthalate final concentration of 250mg/L, 500mg/
L, in the minimal medium of 1000mg/L, 1500mg/L, seed liquor, in 30 DEG C, 180rmin are accessed by 5% inoculum concentration-1Shake
Culture is swung, the influence of initial substrate concentration and inoculum concentration to bacterial strain WL-3 degradation diethyl terephthalate is measured.
(5) bacterial strain WL-3 measures the degradation capability of terephthalic acid (TPA): respectively in final concentration of 2000mg/L containing to benzene
In the minimal medium of dioctyl phthalate, seed liquor, in 30 DEG C, 200rmin are accessed by 5% inoculum concentration-1Shake culture measures bacterium
Degradation capability of the strain WL-3 to terephthalic acid (TPA).
The utility model has the advantages that using wastewater from chemical industry as separation material, isolate and purify can be with efficient degradation to benzene two to one plant by the present invention
The microorganism of formic acid diethylester and its metabolite has realistic meaning to PET biodegrade and the purification of industrial wastewater very much.
The bacterial strain has degradation effect to the metabolite terephthalic acid (TPA) of diethyl terephthalate, is suitably applied in industrial wastewater
On biological treating.
Detailed description of the invention
Fig. 1 a is the effect picture of the diethyl terephthalate HPLC of non-inoculating strain WL-3.
Fig. 1 b is the degradation effect figure of diethyl terephthalate HPLC after inoculating strain WL-3 tetra- days.
Fig. 1 c is the degradation effect figure of diethyl terephthalate HPLC after inoculating strain WL-3 seven days.
Fig. 2 a is the influence schematic diagram that temperature grows bacterial strain WL-3.
Fig. 2 b is the influence schematic diagram that pH value grows bacterial strain WL-3.
Fig. 2 c is the influence schematic diagram that liquid amount grows bacterial strain WL-3.
Fig. 2 d is the influence schematic diagram that revolving speed grows bacterial strain WL-3.
Fig. 3 is diethyl terephthalate degradation curve and bacterial strain WL-3 growth curve.
Fig. 4 a is influence schematic diagram of the temperature to bacterial strain WL-3 degradation diethyl terephthalate.
Fig. 4 b is influence schematic diagram of the pH value to bacterial strain WL-3 degradation diethyl terephthalate.
Fig. 4 c is influence schematic diagram of the initial substrate concentration to bacterial strain WL-3 degradation diethyl terephthalate.
Fig. 4 d is influence schematic diagram of the inoculum concentration to bacterial strain WL-3 degradation diethyl terephthalate.
Fig. 5 a is the effect picture of the terephthalic acid (TPA) HPLC of non-inoculating strain WL-3.
Fig. 5 b is the degradation effect figure of inoculating strain WL-3 terephthalic acid (TPA) HPLC two days later
Fig. 6 terephthalic acid (TPA) degradation curve.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies content described in example and is merely to illustrate the present invention, without sheet described in detail in claims should will not be limited
Invention.
Experimental method used in following embodiments is conventional method without specified otherwise.Institute in following embodiments
Experiment reagent consumptive material used etc. can be bought without specified otherwise from commercial use.
Embodiment 1
The separation screening of diethyl terephthalate degradation bacteria strains Delftia WL-3:
Take 5mL sewage sample to be placed in the enriched medium of 100mL diethyl terephthalate containing 500mg/L, in 30 DEG C,
180r·min-1Cultivate 7d.With ultraviolet scanner measurement pregnant solution to the degradation situation of diethyl terephthalate, determine to benzene
After dicarboxylate is degraded, the enriched medium of diethyl terephthalate containing 750mg/L is linked into 10% inoculum concentration
In, continue to be enriched with and measure degradation situation, according to said method until diethyl terephthalate concentration is improved to 1500mg/L, and pass
Generation 3 times.
Diethyl terephthalate pregnant solution is coated on 1000mg/L diethyl terephthalate after gradient dilution is
On the inorganic salts plate of sole carbon source, 3~4d is cultivated respectively in 30 DEG C of incubators.By the different single colonie difference of colonial morphology
The purifying of LB plate is lined, and is inoculated in the liquid inorganic salt culture using 500mg/L diethyl terephthalate as sole carbon source
In base, in 30 DEG C, 180rmin-1After shaking table culture 7d, take 1mL sample that supernatant is collected by centrifugation, with high-efficient liquid phase chromatogram HPLC come
Detect the content of diethyl terephthalate.It is found by high-efficient liquid phase chromatogram HPLC, compares, compile with the shaking flask that bacterium is not added is compareed
Number it can make the peak-fall of diethyl terephthalate for the bacterial strain of WL-3.
Above-mentioned minimal medium is by ammonium nitrate 1.0g, sodium chloride 1.0g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate
1.5g adds water to 1.0L preparation, adjusts pH value to 7.0, agar 20.0g, 121 DEG C of sterilizing 20min is added in solid medium, uses
Preceding addition diethyl terephthalate is as carbon source.
Above-mentioned LB culture medium is to be added water to 1.0L preparation by peptone 10.0g, yeast powder 5g, sodium chloride 5.0g, adjusted pH
For value to 7.0, agar 20.0g, 121 DEG C of sterilizing 15min is added in solid medium.
The medium centrifugal before and after diethyl terephthalate that WL-3 is degraded takes the methanol dilution that 10mL is used after supernatant, mistake
The organic filter membrane of 0.22um, sample are used for high performance liquid chromatography detection (HPLC).Terephthalic acid (TPA) in the control culture solution of bacterium is not connect
The retention time of diethylester is 3.29min or so, and after inoculating strain WL-3 and culture for 24 hours, terephthalic acid (TPA) two in culture solution
The content of ethyl ester is decreased obviously, and detects the mesostate new peak of 4.2min or so.Specific chromatographic condition are as follows: chromatography
Column is C18kromasil 250mm × 4.6mm；Column temperature is room temperature；Pump p680 HPLC pump；Detector UVD170U；Detect wave
Long 240nm；Mobile phase methanol 80%；Flow velocity 1ml/min. (Fig. 1 a and Fig. 1 b).
Embodiment 2
The identification and its growth characteristics of diethyl terephthalate and its metabolite degradation bacteria strains Delftia WL-3:
The identification of WL-3:
16S rDNA identification is carried out to WL-3: utilizing primer 2 7F:5`-AGAGTTTGATCCTGGCTCAG-3` and 1492R:
5`-TACCTTGTTACGACTT-3` expands the 16S rDNA of bacterial strain WL-3, is connected to cloning vector in such a way that T/A is cloned
PMD19T constructs recombinant cloning vector pMD19T-16S, is transformed into cloning host bacterium Escherich coli DH5 α acquisition
Recombinant microorganism Escherich coli DH5 α (pMD19T-16S) surveys recombinant microorganism exogenous sequences obtained
Sequence, ncbi database compare the 16S rDNA sequence, on a molecular scale identify bacterial strain WL-3 to Delftiatsuruhatensis category,
The nucleotide sequence of 16S rDNA is as shown in SEQ ID NO:1 in sequence table.
The growth characteristics of WL-3:
Delftia WL-3 is grown relatively slowly on LB plate, and 30 DEG C, 48h can form the milky bacterium colony that diameter is 2mm, bacterium
Neat in edge is fallen, it is glossy, it is prominent, it is sticky, it moistens, it is smooth, it is opaque, it is special based on its 16S rDNA sequence and Physiology and biochemistry
Sign, bacterial strain WL-3 are accredited as Delftia category.
The optimum growth temperature of bacterial strain WL-3 is 30 DEG C, also can be very good to grow at 37 DEG C, but at (20 DEG C of lower temperature
With 25 DEG C) and when higher temperature (42 DEG C), growth is then obviously inhibited.Bacterial strain WL-3 is when pH is 6 and 7, well-grown,
Its most suitable growth pH is 6；When pH is 4 and 10, growth is just obviously inhibited, as shaking flask fills liquid when bacterial strain WL-3 is grown
The decline of its increment of the increase of amount, shows that bacterial strain WL-3 is also aerobic microorganism, as shown in Figure 2 a-2d.
Embodiment 3
The degradation characteristic of diethyl terephthalate and its metabolite degradation bacteria Delftia WL-3:
From picking bacterial strain WL-3 single colonie on LB plate, it is inoculated in 3mL LB liquid medium respectively in 30 DEG C, shaking table
180r·min-1Cultivate 12h.Then the culture solution of the bacterial strain is transferred in the fresh LB liquid medium of 100mL, continues to train
Support 12h.6000r·min-1It is centrifuged 10min, thallus is collected and OD is made after being washed twice with the minimal medium of sterilizing600nm
=1.0 bacteria suspension, i.e. seed liquor.In the minimal medium of the final concentration of 1000mg/L of diethyl terephthalate, press
5% inoculum concentration accesses OD600nm=1.0 bacterial strain WL-3 seed liquors, in 30 DEG C, 180rmin-1Shake culture is sampled every for 24 hours
Once, OD is measured600nmWith the concentration of diethyl terephthalate.
Bacterial strain WL-3 is inoculated into 1d in culture medium it can be seen from Fig. 4 a~Fig. 4 d, begins to degradation terephthalic acid (TPA) two
Ethyl ester, without apparent retardation phenomenon；Degradation rate gradually increases later, to 7d just by the terephthalic acid (TPA) of 1000mg/L
Diethylester is degraded completely substantially.In addition, from this figure it can be seen that with diethyl terephthalate degradation, bacterial strain WL-3
Increment start to gradually increase；After diethyl terephthalate is degradable, the increment of bacterial strain WL-3 also reaches most
Greatly.It should be the result shows that bacterial strain WL-3 can use diethyl terephthalate is grown.
For bacterial strain WL-3 in 30 DEG C and 37 DEG C, the degradation rate of diethyl terephthalate is essentially identical；And at 25 DEG C, it is right
The degradation rate of diethyl phthalate is just obviously inhibited；When temperature is 42 DEG C, almost non-degradable terephthalic acid (TPA) diethyl
Ester (Fig. 4 a).Bacterial strain WL-3 is very wide to the adaptation range of pH when degrading diethyl terephthalate, has between pH 5-9
There is good effect；Even if (degradation rate also can achieve 58% to diethyl terephthalate when pH is 4.By this result with
The growth pH of bacterial strain WL-3 is compared, it is found that its pH range of degrading than wide more of growth pH range, illustrates the drop that bacterial strain WL-3 is generated
Solving enzyme has stronger pH adaptability (Fig. 4 b).Bacterial strain WL-3 is to the degradation rate of diethyl terephthalate with concentration of substrate
Increase and reduce.It is 97.5% in the degradation rate of 7d when diethyl terephthalate initial concentration is 500mg/L；And
When diethyl terephthalate initial concentration is 1000mg/L, 92.3% still can achieve in the degradation rate of 7d, explanation
The bacterial strain has efficient degradation efficiency (Fig. 4 c) to diethyl terephthalate.With the increase of inoculum concentration, terephthalic acid (TPA)
The degradation rate of diethylester obviously increases.When inoculum concentration is 1%, degradation rate of the diethyl terephthalate in 7d is
42.3%；And when inoculum concentration is 5% and 10%, diethyl terephthalate is degraded in 3d and 6d completely substantially respectively
(Fig. 4 d).
Embodiment 4
Degradation of the diethyl terephthalate degradation bacteria Delftia WL-3 to terephthalic acid (TPA):
After 48 hours, find that this material concentration is especially low (Fig. 5 a and Fig. 5 b) with HPLC detection terephthalic acid (TPA), it therefore, can
To think that bacterial strain WL-3 can be with the above-mentioned substrate of permineralization.As seen from Figure 6, bacterial strain WL-3 is inoculated into terephthaldehyde's concentration and is
2h begins to degradation terephthalic acid (TPA) in the minimal medium of 2000mg/L, and degradation rate gradually increases later, has arrived 48h just
The terephthalic acid (TPA) of 2000mg/L is degraded completely substantially.
SEQUENCE LISTING
<110>Nanjing University of Technology
<120>one plants degradation diethyl terephthalate bacterial strain and its application
<130> SG170314002
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1398
<212> DNA
<213> Delftiamobilis
<400> 1
agcgccctcc ttgcggttag gctacctact tctggcgaga cccgctccca tggtgtgacg 60
ggcggtgtgt acaagacccg ggaacgtatt caccgcggca tgctgatccg cgattactag 120
cgattccgac ttcacgcagt cgagttgcag actgcgatcc ggactacgac tggttttatg 180
ggattagctc cccctcgcgg gttggcaacc ctctgtacca gccattgtat gacgtgtgta 240
gccccaccta taagggccat gaggacttga cgtcatcccc accttcctcc ggtttgtcac 300
cggcagtctc attagagtgc tcaactgaat gtagcaacta atgacaaggg ttgcgctcgt 360
tgcgggactt aacccaacat ctcacgacac gagctgacga cagccatgca gcacctgtgt 420
gcaggttctc tttcgagcac gaatccatct ctggaaactt cctgccatgt caaaggtggg 480
taaggttttt cgcgttgcat cgaattaaac cacatcatcc accgcttgtg cgggtccccg 540
tcaattcctt tgagtttcaa ccttgcggcc gtactcccca ggcggtcaac ttcacgcgtt 600
agcttcgtta ctgagaaaac taattcccaa caaccagttg acatcgttta gggcgtggac 660
taccagggta tctaatcctg tttgctcccc acgctttcgt gcatgagcgt cagtacaggt 720
ccaggggatt gccttcgcca tcggtgttcc tccgcatatc tacgcatttc actgctacac 780
gcggaattcc atccccctct accgtactct agccatgcag tcacaaatgc agttcccagg 840
ttgagcccgg ggatttcaca tctgtcttac ataaccgcct gcgcacgctt tacgcccagt 900
aattccgatt aacgctcgca ccctacgtat taccgcggct gctggcacgt agttagccgg 960
tgcttattct tacggtaccg tcatgggccc cctgtattag aaggagcttt ttcgttccgt 1020
acaaaagcag tttacaaccc gaaggccttc atcctgcacg cggcattgct ggatcaggct 1080
ttcgcccatt gtccaaaatt ccccactgct gcctcccgta ggagtctggg ccgtgtctca 1140
gtcccagtgt ggctggtcgt cctctcagac cagctacaga tcgtcggctt ggtaagcttt 1200
tatcccacca actacctaat ctgccatcgg ccgctccaat cgcgcgaggc ccgaaggtcc 1260
cccgctttca tcctcagatc gtatgcggta ttagctactc tttcgagtag ttatccccca 1320
cgactgggca cgttccgatg tattactcac ccgttcgcca ctcgtcagcg tccgaagacc 1380
tgttaccgtt cgactgca 1398
Claims (3)
1. the bacterial strain of one plant of degradation diethyl terephthalate, classification naming is Delftiatsuruhatensis (Delftia sp.), bacterial strain
Number it is WL-3, has been preserved in China typical culture collection center, deposit number is CCTCC NO:M 2017136, preservation date
For on March 21st, 2017.
2. application of the bacterial strain described in claim 1 in degrading waste water in diethyl terephthalate.
3. application according to claim 2, which is characterized in that the concentration range of diethyl terephthalate is 250mg/L
~1500mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710328523.2A CN106916773B (en) | 2017-05-10 | 2017-05-10 | One plant degradation diethyl terephthalate bacterial strain and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710328523.2A CN106916773B (en) | 2017-05-10 | 2017-05-10 | One plant degradation diethyl terephthalate bacterial strain and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106916773A CN106916773A (en) | 2017-07-04 |
| CN106916773B true CN106916773B (en) | 2019-04-12 |
Family
ID=59568937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710328523.2A Active CN106916773B (en) | 2017-05-10 | 2017-05-10 | One plant degradation diethyl terephthalate bacterial strain and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916773B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343637B (en) * | 2019-06-28 | 2021-06-08 | 浙江工业大学 | Enterobacter HY1 and application thereof in degradation of dihydroxyethyl terephthalate |
| CN110734881B (en) * | 2019-11-21 | 2021-11-23 | 江南大学 | Petroleum-eating microbacterium capable of degrading polyethylene glycol terephthalate and intermediate thereof |
| CN113980839B (en) * | 2021-10-14 | 2023-04-18 | 华南农业大学 | Delftia sp NLG11 for degrading tobacco nicotine and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004055218A1 (en) * | 2002-12-18 | 2004-07-01 | Ufz-Umweltforschungs Zentrum Leipzig-Halle Gmbh | Bacterial strains of delftia acidovorans mc1-r, their production and their use in the production of the r enantiomers of 2-phenoxypropionic acid derivatives |
| CN101947380A (en) * | 2010-08-31 | 2011-01-19 | 天津工业大学 | Method for degrading polyethylene terephathalate |
| CN101993838A (en) * | 2010-07-08 | 2011-03-30 | 浙江工业大学 | Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof |
| CN104630112A (en) * | 2015-02-10 | 2015-05-20 | 绍兴柯桥江滨水处理有限公司 | Acinetobacter as well as preparation method and application of acinetobacter |
| CN105907688A (en) * | 2016-06-27 | 2016-08-31 | 南京工业大学 | Strain for degrading phenol compounds and application of strain |
-
2017
- 2017-05-10 CN CN201710328523.2A patent/CN106916773B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004055218A1 (en) * | 2002-12-18 | 2004-07-01 | Ufz-Umweltforschungs Zentrum Leipzig-Halle Gmbh | Bacterial strains of delftia acidovorans mc1-r, their production and their use in the production of the r enantiomers of 2-phenoxypropionic acid derivatives |
| CN101993838A (en) * | 2010-07-08 | 2011-03-30 | 浙江工业大学 | Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof |
| CN101947380A (en) * | 2010-08-31 | 2011-01-19 | 天津工业大学 | Method for degrading polyethylene terephathalate |
| CN104630112A (en) * | 2015-02-10 | 2015-05-20 | 绍兴柯桥江滨水处理有限公司 | Acinetobacter as well as preparation method and application of acinetobacter |
| CN105907688A (en) * | 2016-06-27 | 2016-08-31 | 南京工业大学 | Strain for degrading phenol compounds and application of strain |
Non-Patent Citations (2)
| Title |
|---|
| Delftia tsuruhatensis sp nov., a terephthalate-assimilating bacterium isolated from activated sludge;Shigematsu, T等;《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》;20030930;第53卷(第5期);第1479-1483页，参见全文 * |
| 一株DMP降解菌的分离鉴定及其降解特性;金德才等;《微生物学通报》;20090920;第36卷(第9期);第1318-1323页，参见全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106916773A (en) | 2017-07-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112481137B (en) | Cladosporium and application thereof in degradation of polyurethane plastic | |
| CN107177532B (en) | A kind of organic nitrogen contaminant degradation microbial inoculum and its application | |
| CN111826310B (en) | Mixed bacterium enzyme preparation containing stenotrophomonas maltophilia and application thereof | |
| CN106916773B (en) | One plant degradation diethyl terephthalate bacterial strain and its application | |
| US20230212620A1 (en) | Stenotrophomonas pavanii capable of degrading polyethylene terephthalate | |
| CN105886422B (en) | The application of bacillus subtilis BY7 and its degrade residual bait and ammonia nitrogen | |
| CN110218682B (en) | Pseudomycosis bacillus and application thereof in sludge reduction | |
| CN110373346B (en) | Bacillus subtilis sp.A-5 and application thereof | |
| CN113502241B (en) | Rose fungus and application thereof | |
| CN106591172B (en) | A kind of Rhodococcus ruber PTA-2 and its immobilization and application | |
| CN111187744A (en) | High-density industrial fermentation medium for stratospheric bacillus and fermentation method thereof | |
| CN105255754A (en) | Bacterium for degrading ammonia nitrogen and total nitrogen and application and processing method thereof | |
| CN115786185A (en) | Bacillus thuringiensis for producing PHA (polyhydroxyalkanoate) by using various cheap substrates and application | |
| CN114214235A (en) | Efficient flocculating bacterium and application thereof in sewage treatment | |
| CN114317324A (en) | Alcaligenes and product and application thereof | |
| CN102965309B (en) | Rhodococcus sp. and application thereof to micro-biologically degrading 4-fluorocinnamic acid | |
| CN116121099B (en) | Mangrove bacillus and application thereof and straw cellulose degradation method | |
| CN117417846B (en) | Bacillus subtilis ZL-1 and application thereof in degradation of plastics | |
| CN104630104A (en) | Novel strain with azo decoloration capacity and application of novel strain | |
| CN113717882B (en) | Geobacillus galactose BWTGW1.1 and application thereof | |
| CN106479920B (en) | One plant of fragrant phenoxy phenoxy propionic acid herbicide degradation bacterial strain and its application | |
| CN115216425B (en) | Flavobacterium nepalensis and application thereof in degradation of plastics | |
| CN116463232B (en) | Glutamine bacillus G2 and application thereof | |
| CN117721053B (en) | Stress-resistant benzoic acid compound degradation strain, microbial inoculum and application thereof | |
| CN114134076B (en) | Bacillus licheniformis for producing extracellular polysaccharide, flocculant and application of flocculant in sewage treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |