CN117721053B - Stress-resistant benzoic acid compound degradation strain, microbial inoculum and application thereof - Google Patents
Stress-resistant benzoic acid compound degradation strain, microbial inoculum and application thereof Download PDFInfo
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- 230000015556 catabolic process Effects 0.000 title claims abstract description 52
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a stress-resistant benzoic acid compound degrading strain, a microbial agent and application thereof, wherein the degrading strain is pseudomonas stutzeri (Pseudomonas stutzeri), the strain is named HMF10 and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 11 months of 2022, and the strain preservation number is: CGMCC No.26066. The strain HMF10 has stronger adaptability and tolerance to living environment, can efficiently degrade benzoic acid compounds in waste water under severe environmental conditions such as acidity, alkalinity, low temperature, high temperature and the like, and solves the problems of strong inhibition effect and low degradation efficiency of functional microorganisms under reversible environmental conditions.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a stress-resistant benzoic acid compound degradation strain, a microbial inoculum and application thereof.
Background
Benzoic acid compounds are intermediate products of biological metabolism of various aromatic compounds, are commonly used in industries of foods, medicines, plastics, dyes and the like, and are important intermediates and raw materials of various fine chemicals. The structure of the benzoic acid compound is aromatic acid with carboxyl on benzene ring, and typical benzoic acid compound and application thereof are as follows: benzoic acid, also known as benzoic acid, has antimicrobial activity as the undissociated acid, is a broad-spectrum antimicrobial agent and has good antimicrobial effect on yeasts, molds and part of bacteria; the p-hydroxybenzoic acid is an organic synthetic raw material with wide application, and is used for the aspects of corrosion prevention, mildew prevention, bactericides and the like of foods, cosmetics and medicines; fluorobenzoic acid, which refers to a benzoic acid derivative having one or more fluorine atoms or one or more trifluoromethyl groups attached to a benzene ring, is the most commonly used non-radioactive chemical tracer in oil fields today; 2-methyl-3-acetoxybenzoic acid (AMBA) is an important chemical intermediate and is widely applied to the fields of chemical industry and the like; benzoic acid herbicide is an important herbicide in the current production, is widely used for preventing and controlling broadleaf weeds in crop fields such as wheat, corn and the like, mainly comprises dicamba, 3, 5-dichlorobenzoic acid and the like, and especially comprises dicamba, and has the characteristics of low price, high efficiency, rapidness, broad spectrum, low residue and the like, and is widely used in all the world.
Although the research on benzoic acid wastewater has been carried out for a long time at present, the research on using biological enhancement to treat benzoic acid-containing wastewater is still less, the application of the benzoic acid-containing wastewater to engineering practice is less, and the benzoic acid wastewater is still difficult to directly carry out aerobic biochemical treatment at present. Aiming at the current situation, strains capable of efficiently degrading the benzoic acid compounds under various reversible conditions are screened and effectively applied to engineering practice, and the strain has become a popular research field of domestic and foreign scholars.
Disclosure of Invention
In order to solve the defects in the prior art, the invention aims to provide a stress-resistant benzoic acid compound degradation strain, a microbial inoculum and application thereof, which can grow by taking benzoic acid compounds in medical and chemical wastewater as unique carbon sources and effectively degrade the benzoic acid compounds.
In order to achieve the above object, the present invention adopts the following technical scheme:
The stress-resistant benzoic acid compound degrading strain is pseudomonas stutzeri (Pseudomonas stutzeri), the strain is named HMF10, and is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 11 and 7 days in 2022, and the strain preservation number is: CGMCC No. 26066.
The application of the degrading strain in degrading benzoic acid compounds in wastewater is that the benzoic acid compounds are one or more of benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid and 3, 6-dichloro-2-methoxybenzoic acid, the concentration of the benzoic acid compounds in the wastewater is 100-1100 mg/L, the temperature of the wastewater is 10-40 , and the pH value of the wastewater is 4-10.
A microbial inoculum prepared from degradation strain. The preparation method of the microbial inoculum comprises the following specific steps:
S1, selecting a bacterial strain HMF10 from an LB solid culture medium, and performing shaking culture on the bacterial strain HMF10 in an LB liquid culture medium until the bacterial strain is in a logarithmic growth phase to obtain bacterial liquid;
s2, inoculating bacterial liquid into a seed tank according to the volume ratio of 5% -10% for culturing;
And S3, inoculating the bacterial liquid cultured in the seed tank into a fermentation tank according to the volume ratio of 5% -10%, and performing expansion culture to obtain the bacterial agent.
Preferably, in the aforementioned step S1, the LB liquid medium comprises the following components: 10g/L peptone, 5g/L yeast extract and 10g/L NaCl; adding agar with the volume ratio of 2% into LB liquid medium to obtain LB solid medium; the culture conditions are as follows: the temperature is 30-35 , and the pH is 7.0-7.5.
Preferably, in the aforementioned step S2, the components of the culture medium in the seed tank are: glucose 8g/L, yeast extract 5g/L, naCl g/L, K 2HPO41g/LCaCO32g/LMgSO4 0.2g/L; the culture conditions are as follows: the temperature is 30-35 , the pH is 7-8, the stirring speed is 180-250 rpm, the dissolved oxygen DO is controlled at 3-4 mg/L, and the time is 24-48 h.
Preferably, in the aforementioned step S3, the composition of the medium in the fermenter is the same as that of the seed tank; the culture conditions are as follows: the temperature is 30-35 , the stirring speed is 180-250 rpm, the dissolved oxygen DO is controlled at 4-6 mg/L, and the time is 48-96 h; the number of thalli after fermentation is more than 10 9/mL.
The application of the microbial inoculum in degrading benzoic acid compounds in wastewater is characterized in that the benzoic acid compounds are one or more of benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid and 3, 6-dichloro-2-methoxybenzoic acid, the concentration of the benzoic acid compounds in the wastewater is 100-1100 mg/L, the temperature of the wastewater is 10-40 , and the pH value of the wastewater is 4-10.
A method for degrading benzoic acid compounds in wastewater by using a microbial inoculum comprises the following specific steps: the microbial inoculum is added into the wastewater according to the inoculation amount of 5-10% by volume ratio, and the mixture is stirred uniformly to degrade.
The device comprises an SBR reactor, wherein the SBR reactor comprises a water distribution tank, a reactor and a water outlet tank which are sequentially connected, the reactor is respectively connected with an aeration system, a PLC automatic control system for detecting the temperature and the pH of the system and a microbial inoculum adding unit, and the microbial inoculum is arranged in the microbial inoculum adding unit. The wastewater in the pharmaceutical and chemical industries is wastewater containing the benzoic acid compounds. The treatment device treats the wastewater as follows:
(1) Supplementing a nitrogen source NH 4SO4 and a phosphorus source KH 2PO4 into the wastewater containing the benzoic acid compounds, and adjusting the chemical oxygen demand, the nitrogen and the phosphorus content in the wastewater to enable the chemical oxygen demand in the wastewater to be: nitrogen: the phosphorus ratio was 300:5:1, uniformly mixing and then introducing the mixture into an SBR reactor;
(2) Adding activated sludge into an SBR reactor, wherein the adding amount is 30% of the volume of wastewater, adding the HMF10 microbial inoculum, adding the degradation microbial inoculum according to 20% of the volume of the activated sludge, controlling the dissolved oxygen in the reactor to be 4-6 mg/L, controlling the pH value to be 7-8, and degrading the dissolved oxygen at the temperature of 30 ;
(3) The concentration of benzoic acid compound in water was measured every 1 cycle (1 d), and water was changed periodically to appropriately supplement nitrogen and phosphorus sources.
Preferably, the concentration of the benzoic acid compound in the wastewater is 100-1100 mg/L, and the addition amount of the microbial inoculum is 10% -20% of the volume of the activated sludge.
The invention has the advantages that: the strain HMF10 has stronger adaptability and tolerance to living environment, can efficiently degrade the benzoic acid compounds in the wastewater under severe environmental conditions such as acidity, alkalinity, low temperature, high temperature and the like, solves the problems of strong inhibition effect and low degradation efficiency of functional microorganisms in the adverse environmental conditions, provides efficient microorganism germplasm resources for degrading the benzoic acid compounds in the wastewater in the medicine and chemical industries, and ensures that the degradation efficiency of the benzoic acid compounds can reach more than 95 percent within 72 hours.
Drawings
FIG. 1 is a colony morphology of strain HMF 10;
FIG. 2 is a photograph of a crystal violet stain of strain HMF 10;
FIG. 3 is a phylogenetic tree of strain HMF 10;
FIG. 4 is a graph showing the degradation effect of strain HMF10 on benzoic acid compounds;
FIG. 5 is a graph showing the effect of different pH values on the degradation of strain HMF 10;
FIG. 6 is a graph showing the effect of different temperatures on the degradation of strain HMF 10;
FIG. 7 is a graph showing the effect of benzoic acid concentration on strain HMF10 degradation;
FIG. 8 is a graph showing the effect of Pseudomonas stutzeri HMF10 on degradation of benzoic acid compounds in actual medical wastewater;
fig. 9 is a graph showing the degradation effect of strain HMF10 on benzoic acid compounds in SBR reactors.
Detailed Description
The invention is described in detail below with reference to the drawings and the specific embodiments.
Example 1: isolation, screening and identification of degradation strains
(1) Screening, separation and purification of bacterial strain HMF10
Taking the activated sludge 10mL of an aeration tank of a wastewater treatment system of a certain pharmaceutical enterprise, placing the activated sludge in an inorganic salt liquid culture medium 90 mL containing p-hydroxybenzoic acid (400 mg/L), and carrying out shaking enrichment culture on a constant-temperature shaking table at 30 and 220 r/min for 7 d. Preparing an inorganic salt culture medium, controlling the concentration of p-hydroxybenzoic acid in the culture medium to be 200mg/L, 500mg/L, 750 mg/L, 900 mg/L and 1000mg/L in sequence, inoculating the culture medium into the inorganic salt liquid culture medium according to the inoculation amount of 5% by volume ratio, and continuously enriching and culturing under the conditions of 30 and 220 r/min. And (3) after a proper amount of enriched bacterial liquid is subjected to gradient dilution, the bacterial liquid is coated on a plate of an inorganic salt solid culture medium containing 1000mg/L of p-hydroxybenzoic acid, the culture is carried out at a constant temperature of 30 , after bacterial colonies grow out, streak separation is carried out, single bacterial colonies are selected and connected to an LB solid culture medium, pure cultures of the bacterial strains are obtained through plate streak separation and purification, the pure cultures are named HMF10, and the pure cultures are preserved in a refrigerator at a temperature of 4 for standby.
Wherein the components of the inorganic salt liquid culture medium are (NH4)2SO41.0 g/LCaCl22H2O 0.04 g/LFeSO47H2O 0.005 g/LNa2HPO412H2O 1.0 g/LMgSO47H2O 0.8 g/L and KH 2PO4 2.0.0 g/L, the pH value is 7.0, 2% of agar is added into the inorganic salt liquid culture medium, namely the inorganic salt solid culture medium, and the culture medium is sterilized at 121 for 30 min.
The LB solid medium comprises the following components: peptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L, pH 7.0, 2% agar was added and the medium was sterilized at 121for 30 min.
(2) Identification of Strain HMF10
Bacterial colony morphology of the strain HMF10 on LB plates was pale yellow, round, smooth-edged, opaque and surface moist (as shown in FIG. 1). The main biological characteristics are: ending flagellum, no spores, short rod shape or micro-arc short rod shape bacteria, gram staining negative (as shown in figure 2), can use benzoic acid compounds as the sole carbon source for growth.
The sequence of the 16S rDNA of the strain is shown in SEQ ID NO. 1, DNA sequences measured by MEGA6 software and the related species of the 16S rDNA gene sequences in a standard database represent the strain, systematic and homologous analysis is carried out, confidence detection is carried out, a bootstrap data set is 1000 times, and a strain phylogenetic tree is drawn (shown in figure 3). The highest homology of the strain HMF10 and the Pseudomonas such as a standard strain Pseudomonas stutzeri CCUG 11256 can reach more than 99%, so the strain HMF10 is identified as Pseudomonas stutzeri (Pseudomonas stutzeri), the strain is named HMF10 and is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.26066, the preservation date is 2022, 11 months and 7 days, and the preservation address is: the institute of microbiology, national academy of sciences, china, the area , west way 1,3, beijing, chaoyang.
Example 2: degradation performance of bacterial strain HMF10 on benzoic acid compounds
The strain HMF10 is inoculated into LB liquid medium, and the components of the LB liquid medium are as follows: and (3) carrying out overnight amplification culture on 10g/L of peptone, 5g/L of yeast extract and 10g/L of NaCl at the temperature of 30 and 220 rpm, centrifuging the rotation speed of the obtained thallus 5000 rpm for 10 min after the thallus enters the late stage of the logarithmic growth phase, discarding the supernatant, suspending the thallus in an inorganic salt liquid culture medium by adopting a vortex vibration method, wherein the components of the inorganic salt liquid culture medium are (NH4)2SO41.0 g/LCaCl22H2O 0.04 g/LFeSO47H2O 0.005 g/LNa2HPO412H2O 1.0 g/LMgSO47H2O 0.8 g/L and KH 2PO4 2.0 g/L, repeating centrifugation and washing for three times, re-suspending the thallus by using the inorganic salt liquid culture medium, detecting an OD600 value, and adjusting the concentration OD600 of the initial bacterial suspension by adjusting the culture medium of an access experiment to be 1.0 to obtain seed liquid.
An inorganic salt liquid culture medium is prepared, the initial concentration of benzoic acid, p-hydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid (AMBA) and 3, 6-dichloro-2-methoxybenzoic acid (dicamba) in the culture medium is 1000mg/L, and the pH value of the culture medium is adjusted to 7. The bacterial suspension with OD600 of 1.0 is inoculated into a prepared culture medium with various benzoic acid organic matters as the only carbon source according to the inoculum size of 5 percent of the volume ratio, and the culture is carried out under the conditions of 30 and 220 rpm. The concentration of the benzoic acid organic matters is detected by sampling every 12 h hours, the degradation rate of each time period is calculated, and the degradation effect is shown in figure 4.
As can be seen from FIG. 4, the strain HMF10 can effectively degrade benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid, 3, 6-dichloro-2-methoxybenzoic acid, and the degradation rates after 72 hours are 97.33%, 95.33%, 98.27%, 97.57 and 98.57%, respectively. The embodiment illustrates that the separated strain HMF10 can utilize benzoic acid organic matters as the only carbon source for growth and propagation, and can effectively degrade benzoic acid compounds in medical wastewater.
Example 3: influence of pH on degradation effects of HMF10 bacteria
The bacterial strain HMF10 bacterial suspension with OD600 of 1.0 is inoculated into an inorganic salt liquid culture medium containing benzoic acid compounds (benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid (AMBA) and 3, 6-dichloro-2-methoxybenzoic acid (dicamba)) according to the volume ratio of 5 percent, the components of the culture medium are (NH4)2SO41.0 g/LCaCl22H2O 0.04 g/LFeSO47H2O 0.005 g/LNa2HPO412H2O 1.0 g/LMgSO47H2O 0.8 g/L and KH 2PO4 of 2.0 g/L, the initial concentration of each benzoic acid organic matter in the culture medium is 500 mg/L, the pH value is regulated by 1 mol/L hydrochloric acid and sodium hydroxide, the pH value is regulated to 2, 3, 4,5, 6, 7, 8, 9, 10, 11 and 12, the culture is carried out under the conditions of 30 and 220 rpm, the concentration of each benzoic acid organic matter is sampled and measured and the degradation rate is calculated after 72 h, and the degradation rate is shown in figure 5.
As shown in FIG. 5, under the condition of an initial pH value of 4-10, the degradation rate of the strain HMF10 on various benzoic acid organic matters is over 85 percent; when the initial pH value is 6-8, the degradation rate of the bacterial strain HMF10 on each pollutant is above 92%, and the degradation efficiency is highest; however, when the pH is lowered to 3 or less and raised to 10 or more, the strain degradation effect is low, and more than 60% is achieved. The embodiment shows that the optimal pH range of the strain HMF10 for degrading benzoic acid organic matters is 6-8, but the degradation efficiency is still higher when the pH is 4-6 and the pH is 8-10 in a reversible environment, and the degradation capacity of the HMF10 on the benzoic acid organic matters can be obviously reduced only in an extremely acidic or extremely alkaline environment with the degradation efficiency being more than 85%.
Example 4: influence of temperature on degradation effect of HMF10 bacteria
The bacterial strain HMF10 bacterial suspension with OD600 of 1.0 is inoculated into an inorganic salt liquid culture medium containing benzoic acid organic matters (benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid (AMBA) and dicamba (3, 6-dichloro-2-methoxybenzoic acid)) according to the inoculation amount of 5% by volume, the components of the culture medium are (NH4)2SO41.0 g/LCaCl22H2O 0.04 g/LFeSO47H2O 0.005 g/LNa2HPO412H2O 1.0 g/LMgSO47H2O 0.8 g/L and KH 2PO4 2.0.0 g/L, the initial concentration of each benzoic acid organic matter in the culture medium is 500 mg/L, the pH is 7, the culture medium is respectively placed in 5 ,10 ,15 ,20 , 25 ,30 , 35 , 40 ,220 and rpm, the concentration of each benzoic acid organic matter is sampled and measured after shaking table h, and the degradation rate is calculated, as shown in figure 6.
As shown in FIG. 6, when the temperature is within the range of 5-30 , the degradation efficiency of the strain HMF10 on various benzoic acid organic matters is gradually increased along with the temperature rise, when the temperature is 10-40 , the degradation rate is more than 80%, and when the temperature is 30 , the degradation rate of each benzoic acid organic matter is highest and is 97.67-99%; when the temperature is within the range of 30-40 , the degradation rate of the bacterial strain HMF10 on various benzoic acid organic matters is gradually reduced, but the degradation rate is still kept above 90%. The embodiment shows that the optimal temperature for degrading benzoic acid organic matters by the strain HMF10 is 25-35 , but the degradation efficiency is still high and is more than 80% in the reversible environment of 10-25 and 35-40 .
Example 5: influence of concentration of benzoic acid organic matters on degradation effect of HMF10 bacteria
Bacterial strain HMF10 bacterial suspension with OD600 of 1.0 is inoculated into inorganic salt culture medium containing benzoic acid organic matters (p-hydroxybenzoic acid) according to the inoculation amount of 5% by volume, the initial concentration of 50mg/L, 100 mg/L, 300 mg/L, 500mg/L, 700 mg/L, 900 mg/L, 1100 mg/L and 1200 mg/L of hydroxybenzoic acid are respectively added into the culture medium, the pH is 7, the culture medium is respectively placed in a shaking table at 30 and 220 rpm for culture, the concentration of p-hydroxybenzoic acid is measured after 72 h sampling, and the degradation rate is calculated, and the result is shown in figure 7.
As shown in FIG. 7, under the optimal condition, the degradation efficiency of the HMF10 strain on the parahydroxybenzoic acid with the initial concentration of 100-500 mg/L is over 90%, and the degradation effect is excellent and is between 85% and 90% when the initial concentration is 700-1100 mg/L. This example demonstrates that high concentrations of benzoic acid compounds do not cause toxic effects on the strain HMF10, demonstrating good adaptation of the strain to high concentration substrate environments.
Example 6: preparation of degradation microbial inoculum
Selecting a bacterial strain HMF10 from an LB solid culture medium, inoculating the bacterial strain HMF10 into an LB liquid culture medium, and performing shake culture at 30 and 220rpm until the bacterial strain HMF is in a logarithmic growth phase; transferring HMF10 bacterial liquid in logarithmic growth phase with transfer volume ratio of 10% into a seed tank for culturing, controlling the temperature of the seed tank to 30deg.C, stirring speed to 220rpm, and controlling dissolved oxygen DO to 4 mg/L. Inoculating the bacterial liquid cultured in the seed tank into a fermentation tank for expansion culture in an inoculum size of 5%, and controlling fermentation conditions: the stirring speed is 220rpm, the culture temperature is 30 , and the dissolved oxygen DO is controlled at 6 mg/L; after the fermentation is finished, the effective viable count in the fermentation liquor is more than 10 9/mL, and the obtained fermentation liquor is taken out of the tank and packaged to obtain the HMF10 degradation microbial inoculum.
The LB liquid culture medium comprises the following components: 10g/L peptone, 5g/L yeast extract and 10g/L NaCl; adding agar with volume ratio of 2% into LB liquid medium to obtain LB solid medium. The culture medium components of the seed tank and the fermentation tank are: glucose 8 g/L, yeast extract 5g/L, naCl g/L, K 2HPO41 g/LCaCO32 g/LMgSO4 0.2.2 g/L, pH 7.0.
Example 7: application of microbial inoculum in actual wastewater treatment
The source of the treated water is the water from a sewage treatment plant containing benzoic acid compounds (1102.58 mg/L, 856.42 mg/L of p-hydroxybenzoic acid, 915.20 mg/L of fluorobenzoic acid, 404.87 mg/L of 2-methyl-3-acetoxybenzoic acid (AMBA) and 298.53 mg/L of dicamba (3, 6-dichloro-2-methoxybenzoic acid), and the pH of the wastewater is about 5; the high-efficiency degradation bacteria HMF10 microbial inoculum obtained in example 5 is inoculated into wastewater according to the inoculum size of 10 percent of the volume ratio, the control group is inoculated with activated sludge in a sewage treatment tank of a former factory, shake cultivation is carried out at 30 and 220 rpm, the concentration of various benzoic acid pollutants is measured after 72 h, and the degradation rate of the benzoic acid compounds is calculated, and the result is shown in figure 8.
As can be seen from FIG. 8, the degradation effect of the original activated sludge on the benzoic acid compounds in the wastewater is poor, the removal rate is only 18% -28.33%, and the removal rates of the benzoic acid, the parahydroxybenzoic acid, the fluorobenzoic acid, the 2-methyl-3-acetoxybenzoic acid (AMBA) and the dicamba (3, 6-dichloro-2-methoxybenzoic acid) in the wastewater are 93%, 90%, 87%, 89.67% and 89.33% respectively after the HMF10 microbial inoculum is added. Therefore, the microbial inoculum produced by the strain HMF10 can be applied to the treatment of medical and chemical wastewater containing benzoic acid compounds.
Example 8: degradation effect of strain HMF10 in SBR reactor
This example shows an operation of treating pharmaceutical and chemical wastewater containing benzoic acid compounds in an SBR reactor with strain HMF 10.
The experimental device of the embodiment is an SBR reactor, and mainly comprises a water distribution tank, a reactor, an aeration system and a PLC automatic control system for monitoring pH change and temperature change in the reactor. Wherein the diameter of the reactor is 12cm, the height is 50cm, and the effective volume is 5L. And under the condition of a PLC automatic control system, the temperature is controlled to be 25-35 , and the pH is controlled to be 7-8. The air compressor provides enough air quantity, so that the waste water in the reactor can be uniformly mixed and oxygen required by the growth and metabolism of immobilized bacteria can be provided. The test adopts an intermittent aeration mode to treat the medical and chemical wastewater containing benzoic acid compounds. The run cycle was 24h, comprising four phases: inlet water 0.5 h, reaction 22 h, rest 1h, outlet water 0.5 h.
The method adopts the water quality of an actual sewage treatment plant to measure that the water contains 1102.58 mg/L of benzoic acid, 856.42 mg/L of parahydroxybenzoic acid, 915.20 mg/L of fluorobenzoic acid, 404.87 mg/L of 2-methyl-3-acetoxybenzoic acid (AMBA) and 298.53 mg/L of dicamba (3, 6-dichloro-2-methoxybenzoic acid). Adding a nitrogen source NH 4SO4, a phosphorus source KH 2PO4, sodium chloride, mgSO 47H2 O and the like into water to regulate COD in the wastewater: n: the concentration of P was 300:5:1.
The wastewater is treated by the SBR reactor, taking the wastewater as an example. Activated sludge is obtained from an aeration tank of a sewage treatment plant in a certain city in Nanjing. The treatment method comprises the following steps:
(1) Uniformly mixing the benzoic acid wastewater and then injecting the mixture into a water distribution tank;
(2) Adding activated sludge into a reactor according to 30% of the volume of wastewater, adding HMF10 degradation microbial inoculum into the reactor according to 20% of the volume of the activated sludge, starting a water pump, and controlling the dissolved oxygen in water to be 4-6 mg/L, the pH value to be 7.0 and the temperature to be 30 through an air compressor;
(3) And (3) measuring the concentration of the benzoic acid compounds in the water sample at intervals of 1 period (1 d) to judge the removal effect of the biochemical system on the benzoic acid compounds in the wastewater, and changing water periodically to properly supplement nitrogen and phosphorus sources.
As can be seen from FIG. 9, in the operation 12d of the SBR reactor, the concentration of the benzoic acid compound in the final effluent of the reactor is below 15 mg/L, and the removal rate is stabilized to be above 95%. The HMF10 degrading bacterial agent has stable degradation on benzoic acid compounds, can maintain stable and efficient operation of an SBR system, and has good development and application values in treating medical and chemical wastewater containing the benzoic acid compounds.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be appreciated by persons skilled in the art that the above embodiments are not intended to limit the invention in any way, and that all technical solutions obtained by means of equivalent substitutions or equivalent transformations fall within the scope of the invention.
Claims (9)
1. The stress-resistant benzoic acid compound degrading strain is pseudomonas stutzeri (Pseudomonas stutzeri), the strain is named HMF10, and is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) at 11 and 7 days of 2022, wherein the strain is provided with a strain preservation number: CGMCC No. 26066.
2. The application of the degradation strain in degrading benzoic acid compounds in wastewater, which is characterized in that the benzoic acid compounds are one or more of benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid and 3, 6-dichloro-2-methoxybenzoic acid, the concentration of the benzoic acid compounds in the wastewater is 100-1100 mg/L, the temperature of the wastewater is 10-40 , and the pH is 4-10.
3. A microbial agent prepared using the degradation strain of claim 1.
4. A method for preparing the microbial inoculum of claim 3, comprising the specific steps of:
S1, selecting a bacterial strain HMF10 from an LB solid culture medium, and performing shaking culture on the bacterial strain HMF10 in an LB liquid culture medium until the bacterial strain is in a logarithmic growth phase to obtain bacterial liquid;
s2, inoculating the bacterial liquid into a seed tank for culture;
And S3, inoculating the bacterial liquid after the seed tank culture into a fermentation tank for expansion culture, and obtaining the bacterial agent after fermentation.
5. The method according to claim 4, wherein in the step S1, the LB liquid medium comprises the following components: 10g/L peptone, 5g/L yeast extract and 10g/L NaCl; adding agar with the volume ratio of 2% into LB liquid medium to obtain LB solid medium; the culture conditions are as follows: the temperature is 30-35 , and the pH is 7.0-7.5.
6. The method according to claim 4, wherein in the step S2, the components of the culture medium in the seed tank are: glucose 8g/L, yeast extract 5g/L, naCl g/L, K 2HPO4 1g/LCaCO3 2g/LMgSO4 0.2g/L; the culture conditions are as follows: the temperature is 30-35 , the pH is 7-8, the stirring speed is 180-250 rpm, the dissolved oxygen DO is controlled at 3-4 mg/L, and the time is 24-48 h.
7. The method according to claim 4, wherein in the step S3, the composition of the medium in the fermenter is the same as that of the seed tank; the culture conditions are as follows: the temperature is 30-35 , the stirring speed is 180-250 rpm, the dissolved oxygen DO is controlled at 4-6 mg/L, and the time is 48-96 h; the number of thalli after fermentation is more than 10 9/mL.
8. The application of the microbial inoculum of claim 3 in degrading benzoic acid compounds in wastewater, wherein the benzoic acid compounds are one or more of benzoic acid, parahydroxybenzoic acid, fluorobenzoic acid, 2-methyl-3-acetoxybenzoic acid and 3, 6-dichloro-2-methoxybenzoic acid, the concentration of the benzoic acid compounds in the wastewater is 100-1100 mg/L, the temperature of the wastewater is 10-40 , and the pH is 4-10.
9. A method for degrading benzoic acid compounds in wastewater by using the microbial inoculum of claim 3, which is characterized by comprising the following specific steps: the microbial inoculum is added into the wastewater according to the inoculation amount of 5-10% by volume ratio, and the mixture is stirred uniformly to degrade.
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| CN114292775A (en) * | 2021-12-17 | 2022-04-08 | 黄河三角洲京博化工研究院有限公司 | Toluene degrading strain and application thereof |
| CN115786179A (en) * | 2022-10-10 | 2023-03-14 | 黄河三角洲京博化工研究院有限公司 | Bacterial strain for degrading o-dichlorobenzene and application thereof |
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| CN114292775A (en) * | 2021-12-17 | 2022-04-08 | 黄河三角洲京博化工研究院有限公司 | Toluene degrading strain and application thereof |
| CN115786179A (en) * | 2022-10-10 | 2023-03-14 | 黄河三角洲京博化工研究院有限公司 | Bacterial strain for degrading o-dichlorobenzene and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 施氏假单胞菌N2降解1-羟基-2萘甲酸的特性及机理研究;王琰, 等;西安建筑科技大学学报(自然科学版);20170228;49(01);第135-140页 * |
| 施氏假单胞菌N2降解酚类污染物的特性及机理研究;赵静;《中国优秀硕士论文全文数据库》》;20140531(第5期);B027-70 * |
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