CN104630112B - One plant of acinetobacter calcoaceticus and preparation method thereof, application - Google Patents
One plant of acinetobacter calcoaceticus and preparation method thereof, application Download PDFInfo
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- CN104630112B CN104630112B CN201510067447.5A CN201510067447A CN104630112B CN 104630112 B CN104630112 B CN 104630112B CN 201510067447 A CN201510067447 A CN 201510067447A CN 104630112 B CN104630112 B CN 104630112B
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- Prior art keywords
- plant
- acinetobacter calcoaceticus
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- waste water
- acinetobacter
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
Abstract
The present invention relates to one plant of acinetobacter calcoaceticus, this plant of entitled acinetobacter calcoaceticus T1 of bacterium classification（Acinetobacter sp.T1）, it is preserved in China typical culture collection center on November 9th, 2014（CCTCC）, preserving number is CCTCC NO.M 2014558.One plant of acinetobacter calcoaceticus is degraded the application of terephthalic acid (TPA) in dyeing waste water.One plant of acinetobacter calcoaceticus of the present invention has more preferable degradation property to the terephthalic acid (TPA) in dyeing waste water, more there is application value；Degradation efficiency is high, and required time is short；There is more preferable degradation effect under aerobic condition, application is wider.
Description
Technical field
The present invention relates to one plant of acinetobacter calcoaceticus and preparation method thereof, application, the terephthaldehyde especially in degrading waste water
The application of acid, belongs to technical field of microbe application.
Background technology
Alkali decrement waste water results from the alkali deweighting technique of dacron, and the main component of alkali decrement waste water is to diformazan
Sour (PTA), ethylene glycol (EG), polyester oligomer and a small amount of auxiliary agent (such as quaternary ammonium salt cationic face activating agent, alkali-resistant penetrant and
N, N- polyoxyethylene pheynylalkylamine) etc., wherein, PTA accounts for the 63%~71% of the total lotuses of COD, is that the characteristic of alkali decrement waste water is dirty
Contaminate thing [1].In alkali decrement waste water, PTA enters environment in " three wastes " form, and (such as PTA produces waste water and given birth to polyester some waste water
Produce waste water) in, PTA at concentrations up to thousands of milligrams per liter [2].PTA has stimulation toxic [3] to fish, to microorganism in water
Regeneration play the role of suppression, have teratogenesis and mutagenesis [4-5] to some animals.Effectively in processing alkali decrement waste water
PTA, is always problem of concern.
The main method of processing PTA waste water is at present：The materializing strategy of charcoal absorption, ozone or Chlorine Dioxide Oxidation
The such as method and biochemical treatment process with aerobic, anaerobism or anaerobic-aerobic PTA in recent years biodegradable treatment technology is by more
Extensive concern, domestic and foreign scholars have carried out substantial amounts of research [6-10] to how to produce waste water using microbiological treatment PTA.
It is numerous research show PTA can by multiple-microorganism decompose and also can as the only carbon source of microorganism that is degraded
Degraded PTA microorganism is widely present in single bacterial strains in soil, river, the activated sludge of sewage treatment plant and compost, mixed
Closing bacterium and activated sludge can be aerobic with the PTA that be degraded under anaerobic condition, and aerobic degradation speed is faster than anaerobic degradation speed
Much.In current existing document report, the bacterium bag that someone screens the PTA that can degrade includes Gram-negative and positive bacillus, eight
Folded coccus, silk bacterium and saccharomycete.Chen Peng et al. has found a kind of Candida, 1400mg/L PTA can be dropped in 72 hours
Solution more than 80%, degradation efficiency is preferable, but degradation rate is slower.
Therefore, in order to solve the above technical problems, it is necessory to provide a kind of one plant of new acinetobacter calcoaceticus and its degrade to benzene
The application of dioctyl phthalate, to overcome the defect of the prior art.
The content of the invention
In order to solve the above technical problems, the first object of the present invention is that one kind is easily obtained, degrade terephthaldehyde for offer
One plant of good and good to dyeing waste water adaptability acinetobacter calcoaceticus of sour effect.
The second object of the present invention is the application for providing one plant of acinetobacter calcoaceticus.
The third object of the present invention is the preparation method for providing one plant of acinetobacter calcoaceticus.
To realize above-mentioned first purpose, the technical scheme that the present invention takes is：One plant of acinetobacter calcoaceticus, its this plant of bacterium systematic name
For acinetobacter calcoaceticus T1（Acinetobacter sp.T1）, it is preserved in on November 9th, 2014 in China typical culture collection
The heart（CCTCC）, preserving number is CCTCC NO. M 2014558.
To realize above-mentioned second purpose, the technical scheme that the present invention takes is：The application of one plant of acinetobacter calcoaceticus, it is used for height
Terephthalic acid (TPA) in efficiency degraded dyeing waste water.
The application of one plant of acinetobacter calcoaceticus of the present invention is further：Its inoculum concentration is 1%, and temperature is 20-40 DEG C, and pH is 6-
8, dyeing waste water（P-phthalic acid at concentration is 100-1000mg/L）, shaking speed is to detect that its drops after 150-200r/min, 24h
Solve efficiency.
To realize above-mentioned 3rd purpose, the technical scheme that the present invention takes is：The preparation method of one plant of acinetobacter calcoaceticus, it is wrapped
Include following processing step：
1）, enrichment：Aeration water is taken, and is added in enriched medium, is stirred under 180r/min speed, 30 DEG C of cultures
Pregnant solution is taken after 1 day, 1 day, and is added in enriched medium, is so repeated 2 times；
2）, screening：Take pregnant solution to be diluted painting flat board, and cultivated 1-2 days with 30 DEG C on screening and culturing medium, until flat
Bacterium colony is grown on plate；
3）, isolate and purify and Molecular Identification：The bacterium colony grown on flat board is rule on beef extract-peptone flat board
Purifying, 30 DEG C of 24-48h of culture choose the single bacterium colony grown and repeat line 3 times；Single bacterium colony is trained bacterium solution and glycerol tube guarantor is made
Deposit；
4）, secondary screening：Degraded culture medium is prepared, the bacterial strain of purifying is inoculated with, inoculum concentration is 1%, the as a child detection degraded of culture 24
The concentration of terephthalic acid (TPA) in culture medium, picks out P-phthalic acid at concentration and declines more bacterial strain, and glycerol tube preservation is made.
The preparation method of one plant of acinetobacter calcoaceticus of the present invention is further：Step 1）In, comprising as follows in enriched medium
Composition（Unit is g/L）：Yeast extract powder 5.0, peptone 10.0, NaCl10.0 adjusts pH to 7.0 with 1mol/L NaOH.
The preparation method of one plant of acinetobacter calcoaceticus of the present invention is further：Step 1）In, the amount of water and enriched medium is
1:100；The amount of pregnant solution and enriched medium is 1:100.
The preparation method of one plant of acinetobacter calcoaceticus of the present invention is further：Step 2）In, comprising as follows in screening and culturing medium
Composition（Unit is g/L）： NH4Cl1.0, KH2PO43.0,Na2HPO47.0, NaCl0.5, MgSO4·7H2O0.25, PTA（To benzene
Dioctyl phthalate）1.0, agar 15 adjusts pH to 7.5 with 1mol/L NaOH.
The preparation method of one plant of acinetobacter calcoaceticus of the present invention can also be：Step 4）In, in degraded culture medium comprising it is following into
Point（Unit is g/L）：NH4Cl1.0, KH2PO43.0,Na2HPO47.0, NaCl0.5, MgSO4·7H2O0.25, PTA（To benzene two
Formic acid）1.0, agar 15 adjusts pH to 7.5 with 1mol/L NaOH.
Compared with prior art, the present invention has the advantages that：
1. one plant of acinetobacter calcoaceticus degraded terephthalic acid (TPA) effect of the present invention is good, required time is short；Can be degraded higher concentration
Terephthalic acid (TPA), application is wider.
2. it is dirty to printing and dyeing in aeration tank activated sludge of the one plant of acinetobacter calcoaceticus of the present invention screened from processing dyeing and printing sewage
Water has more preferable adaptability, more there is application value.
Embodiment
The present invention is one plant of acinetobacter calcoaceticus, this plant of entitled acinetobacter calcoaceticus T1 of bacterium classification（Acinetobacter sp.T1）,
China typical culture collection center is preserved on November 9th, 2014（CCTCC）, preserving number is CCTCC NO. M
2014558, preservation address：China, Wuhan, Wuhan University.
Above-mentioned acinetobacter calcoaceticus has following feature：Bacterium colony is white, circular, and neat in edge, surface is smooth, moistening.It is micro-
Microscopic observation is shaft-like, no gemma, atrichia, Gram-negative.
Above-mentioned one plant of acinetobacter calcoaceticus can be used for specific pollutants terephthalic acid (TPA) in degraded dyeing waste water, one of which degraded
Method is：Its inoculum concentration is 1%, and temperature is 20-40 DEG C, and pH is 6-8, dyeing waste water（P-phthalic acid at concentration is 100-
1000mg/L）, shaking speed is to detect its degradation efficiency after 150-200r/min, 24h.
Above-mentioned one plant of acinetobacter calcoaceticus can be adopted to be made with the following method：
1）, enrichment：Aeration water is taken in aeration tank, and is added in enriched medium, is stirred under 180r/min speed,
30 DEG C of cultures take pregnant solution after 1 day, 1 day, and add in enriched medium, are so repeated 2 times；Wherein, wrapped in enriched medium
Containing following composition（Unit is g/L）：Yeast extract powder 5.0, peptone 10.0, NaCl10.0 adjusts pH extremely with 1mol/L NaOH
7.0；The amount of water and enriched medium is 1:100；The amount of pregnant solution and enriched medium is 1:100；
2）, screening：Take pregnant solution to be diluted painting flat board, and cultivated 1-2 days with 30 DEG C on screening and culturing medium, until flat
Bacterium colony is grown on plate；Wherein, following composition is included in screening and culturing medium（Unit is g/L）： NH4Cl1.0, KH2PO43.0,
Na2HPO47.0, NaCl0.5, MgSO4·7H2O0.25, PTA（Terephthalic acid (TPA)）1.0, agar 15 adjusts pH with 1mol/L NaOH
To 7.5；
3）, isolate and purify and Molecular Identification：The bacterium colony grown on flat board is rule on beef extract-peptone flat board
Purifying, 30 DEG C of 24-48h of culture choose the single bacterium colony grown and repeat line 3 times；Single bacterium colony is trained bacterium solution and glycerol tube guarantor is made
Deposit；
4）, secondary screening：Degraded culture medium is prepared, the bacterial strain of purifying is inoculated with, inoculum concentration is 1%, the as a child detection degraded of culture 24
The concentration of terephthalic acid (TPA) in culture medium, picks out P-phthalic acid at concentration and declines more bacterial strain, and glycerol tube preservation is made；Its
In, include following composition in degraded culture medium（Unit is g/L）：NH4Cl1.0, KH2PO43.0,Na2HPO47.0, NaCl0.5,
MgSO4·7H2O0.25, PTA（Terephthalic acid (TPA)）1.0, agar 15 adjusts pH to 7.5 with 1mol/L NaOH.
Embodiment above is only the preferred embodiment of this creation, all in this wound not to limit this creation
Any modification, equivalent substitution and improvements for being done etc. within the spirit and principle of work, should be included in this creation protection domain it
It is interior.
Claims (2)
1. one plant of acinetobacter calcoaceticus T1 application, it is characterised in that：For high efficiency degrade dyeing waste water in terephthalic acid (TPA), this
The entitled acinetobacter calcoaceticus of strain bacterium classification（Acinetobacter sp.）, it is preserved in Chinese Typical Representative culture on November 9th, 2014
Collection（CCTCC）, preserving number is CCTCC NO. M 2014558.
2. one plant of acinetobacter calcoaceticus T1 as claimed in claim 1 application, it is characterised in that：Its inoculum concentration is 1%, and temperature is 20-
40 DEG C, pH is 6-8, and P-phthalic acid at concentration is 100-1000mg/L dyeing waste water, and shaking speed is 150-200r/min,
Its degradation efficiency is detected after 24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510067447.5A CN104630112B (en) | 2015-02-10 | 2015-02-10 | One plant of acinetobacter calcoaceticus and preparation method thereof, application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510067447.5A CN104630112B (en) | 2015-02-10 | 2015-02-10 | One plant of acinetobacter calcoaceticus and preparation method thereof, application |
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| CN104630112A CN104630112A (en) | 2015-05-20 |
| CN104630112B true CN104630112B (en) | 2017-09-29 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106916773B (en) * | 2017-05-10 | 2019-04-12 | 南京工业大学 | One plant degradation diethyl terephthalate bacterial strain and its application |
| CN108359617B (en) * | 2017-12-29 | 2021-03-02 | 浙江双良商达环保有限公司 | Acinetobacter CL05 and application thereof in village and town sewage dephosphorization treatment |
| CN108774625B (en) * | 2017-12-29 | 2021-02-23 | 浙江双良商达环保有限公司 | Acinetobacter CL04 and application thereof in village and town sewage dephosphorization treatment |
| CN109576170A (en) * | 2018-08-30 | 2019-04-05 | 常州大学 | One plant height imitates application of the sulfur oxidizing bacterium in Containing Sulfur Black wastewater treatment |
| CN110283745B (en) * | 2019-06-27 | 2021-05-11 | 浙江工业大学 | Acinetobacter hospital FK2 and application thereof in degrading organic pollutants |
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Granted publication date: 20170929 Termination date: 20220210 |