CN103756928A - Bacterial strain for degradation of p-xylene and culture method and application thereof - Google Patents
Bacterial strain for degradation of p-xylene and culture method and application thereof Download PDFInfo
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Abstract
The invention discloses a bacterial strain for degradation of p-xylene and a culture method and application thereof, the bacterial strain is named as Pandoraea sp WL1, the bacterial strain is preserved in the China General Microbiological Culture Collection Center(CGMCC), wherein the accession number is CGMCC NO.7897, and the accession date is July 8th, 2013. The bacterial strain for the degradation of the p-xylene is aerobic-type gram-negative bacteria, can grow by use of the p-xylene as a unique carbon source and energy, and can be completely mineralized into carbon dioxide and water; under the pure culture condition, the bacterial strain can degrade the p-xylene under the conditions of the temperature of 25-35 DEG C and the pH value of 4 to 8; the bacterial strain has good adaptive capacity to a substrate and the broadness to the substrate, can simultaneously degrade the p-xylene and methylbenzene. The bacterial strain lays a foundation for the industrial application of a biological method for disposing organic waste gases.
Description
Technical field
The present invention relates to microorganism strains field, be specifically related to a kind of bacterial strain for the p-Xylol of degrading and cultural method and application.
Background technology
Current, the caused environmental problem of organic exhaust gas and the potential health problem of the atm number lower concentration that Fine Chemical produces receive publicity just day by day.The volatile organic matter (VOCs) that Fine Chemical produces, of a great variety because of it, distribute wide, toxicity is large, there is the features such as wide, large-minded, concentration is low of polluting, pollute the problem complexity that causes, the atmospheric polluting material that the later second largest class of dust that has been regarded as continuing has a large capacity and a wide range simultaneously.According to the reckoning in industry, the total industrial VOCs annual emissions in the whole nation should, more than 2,000 ten thousand tons, meet or exceed national NO at present
xemission level, and along with the development of national economy presents ever-increasing trend.According to statistic datas in 2008 of Environmental Protection in America general bureau (EPA), the VOCs emission level of the U.S. has exceeded 1,378 ten thousand tons.The VOCs of industrial discharge has certain environmental toxicity mostly, as: poisonous, foul smelling, generation photo-chemical smog, damage the ozone layer, cause Greenhouse effect and acid rain etc.; Part even has " three cause " effect, is directly detrimental to health.
Dimethylbenzene (Xylene), as a kind of conventional fine chemical material and organic solvent, mainly comes from the petroleum refinery process of petroleum chemical industry; Wherein, approximately 45% xylol is for the production of p-Xylol (P-xylene), and other purposes also comprise as organic solvent uses (5.2%), produces o-Xylol (7.7%), produces m-xylene (2.0%) and blend gasoline (39%) etc.P-Xylol is a kind of important Organic Chemicals, mainly for the production of pure terephthalic acid (PTA) or dimethyl terephthalate (DMT) (DMT), PTA or DMT generate polyethylene terephthalate (PET) with glycol reaction again, it is polyester, further polyster fibre is produced in processing spinning, polyester cord and polyester bottles, polyester film, polyblend and other industrial element etc. for tire industry; In addition, p-Xylol also has purposes in medication chemistry industry.P-Xylol is as a kind of common volatile organic matter (saturated vapor pressure 8.84mmHg, 25 ℃), p-Xylol has higher toxicity and potential carcinogenic, mutagenesis, is also one of 189 kinds of preferential poisonous air pollutant of controlling that stipulate in U.S. EPA < < clean air amendment (1990) > > simultaneously.In recent years, the environmental problem producing because of p-Xylol project causes Mass disturbance and also happens occasionally in China.What in the production and application process of p-Xylol, produce is a large amount of containing p-Xylol organic exhaust gas, can produce very important impact to the people's healthy and ecotope around; Thereby, extremely urgent containing the research and development of p-Xylol organic exhaust gas control techniques.
Treatment effect is good owing to having for biological process, investment and working cost is low, the gentle (normal temperature of reaction conditions, normal pressure), non-secondary pollution, be easy to the advantages such as bookkeeping, especially more aobvious its economy and superiority when processing the organic exhaust gas of large flow, lower concentration, thereby be subject to increasing attention, current China also industrial application to a certain extent.Seed selection for the high-effective microorganism bacterial strain of specific volatile organism (as benzene homologues, hydrochloric ether and stench class) is most important for treatment effect and the operation steady in a long-term of organic exhaust gas biological treatment device; And for the screening and separating of p-Xylol degradation bacteria strains, in Present Domestic VOCs treatment field, still belong to the starting stage specially.
In the outer research of Present Domestic, actually rare for the report that carries out efficient degrading bacterial strain isolation identification containing p-Xylol organic exhaust gas.2010, Amelia-Elena Rotaru (FEMS Microbiology Ecology, 2010,71:460-468) etc. from the denitrogenation flora of tap water anaerobic environment, isolate and can utilize the microorganism species of p-Xylol as sole carbon source and electron donor simultaneously; 2012, the Wei Xin of Wuhan University Of Technology (chemical industry environmental protection, 2012,6:498-501) etc. isolated a strain and can utilize the pseudomonas of p-Xylol as sole carbon source from certain petrochemical wastewater active sludge, for the improvement of petrochemical wastewater and coking chemical waste water; But above research is not optimized analysis to culture condition, the separation purification method of relevant bacterial strain, do not relate to the processing containing p-Xylol organic exhaust gas simultaneously yet.
Therefore, screening and separating also utilizes directed acclimation method to obtain the efficient degrading bacterial strain of degradable p-Xylol, and analyze its degradation characteristic, thereby for utilizing biologic treating technique to carry out Fine Chemical, containing the improvement of p-Xylol organic exhaust gas, provide certain basic data, there is certain prospects for commercial application.
Summary of the invention
The invention provides a kind of bacterial strain for the p-Xylol of degrading, this bacterial strain p-Xylol of can effectively degrading.
For a bacterial strain for the p-Xylol of degrading, called after Pan Duola bacterium (Pandoraea sp.) WL1, preserving number is CGMCC NO.7897.
The concrete preservation of this bacterial strain is as follows:
Title: Pan Duola bacterium (Pandoraea sp.) WL1;
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, be called for short CGMCC;
The address of depositary institution: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica;
Preservation date: on 07 08th, 2013;
Deposit number: CGMCC NO.7897.
Pan Duola bacterium (Pandoraea sp.) WL1 belongs to Pandoraea and belongs to; Bacterium colony is roundlet shape, more transparent, and the smooth of the edge is neat, diameter 0.2mm left and right; Somatic cells is elongated rod shape, 2.0 μ m left and right, and the raw flagellum of end, has folder film, without gemma; For aerobic type Gram-negative bacteria.
The present invention also provides a kind of cultural method of the bacterial strain for the p-Xylol of degrading, from pharmaceutical and chemical enterprises, extract active sludge, this mud sample carries out after the directed domestication of aeration through passing into for a long time gaseous pollutant (p-Xylol), bacterium liquid is coated and on solid medium flat board, separated single bacterium colony, be placed in minimal medium and add target contaminant (p-Xylol) and carry out separation and purification, repeat screening and separating and obtain a strain and have the aimed strain of efficient degradation characteristic.
For a cultural method for the bacterial strain of the p-Xylol of degrading, comprise the following steps:
1) get the active sludge of pharmaceutical factory's Sewage outlet, pass into p-Xylol and carry out aeration domestication, active sludge, minimal medium and p-Xylol are joined in culturing bottle after stable, in shaking table, cultivate, obtain microflora;
2) utilize R2A solid medium in conjunction with method of dilution butteron on plate and method of scoring, to carry out the purifies and separates of aimed strain, obtain the bacterial strain for the p-Xylol of degrading.
Pharmaceutical factory is Dongyang City Zhejiang, the Zhejiang Province abundant Biology Pharmacy Co., Ltd of Pu Luokang.
The volume ratio of described active sludge, minimal medium and p-Xylol is 1:30～70:0.005～0.05.
Further, the volume ratio of described active sludge, minimal medium and p-Xylol is 1:50:0.01～0.02.
In minimal medium 1L, described minimal medium is comprised of the component of following weight:
In R2A solid medium 1L, described R2A solid medium is comprised of the component of following weight:
Utilize R2A solid medium in conjunction with method of dilution butteron on plate and method of scoring, to carry out the purifies and separates of aimed strain, the R2A of the microflora solid medium aseptic deionized water that soon step 1) will obtain carries out serial dilution by 10 times of dilution methods, gets 10
-4to 10
-8the bacterium liquid of extension rate is coated the flat board of R2A solid medium, be inverted in constant incubator, cultivate 2～3 days for 30 ℃, picking list bacterium colony is within containing the culturing bottle of minimal medium, add p-Xylol to investigate its degradation effect, to there is again efficient degradation effect bacterium liquid and carry out purifying again, so repeat some all after dates (3～5 times), according to the diversity judgement of the growing state of bacterium colony on flat board, apparent characteristic, obtain the pure bacterial strain that a strain degradation effect is good, for the bacterial strain of the p-Xylol of degrading.
The present invention can be used for processing the organic exhaust gas containing p-Xylol for the bacterial strain of the p-Xylol of degrading.Be specially the present invention for the inoculation of the p-Xylol of degrading to the VOCs treatment that contains p-Xylol in bio-trickling device, through biofilm, tame to the steady stage, obtained good p-Xylol removal effect.
The present invention for the strains for degrading p-Xylol of p-Xylol of degrading at 25～35 ℃, under the condition of pH=4～8, carry out, preferably at 30～35 ℃, under the condition of pH=6～7, carry out, can be by different starting point concentration (0～70mg/L in minimal medium in 90h, with the volume of culturing bottle, calculate) the p-Xylol mineralising of thoroughly degrading, there is stronger substrate adaptive faculty; This bacterial strain also has the wide in range property of good substrate, can be simultaneously using benzene homologues such as p-Xylol, toluene as carbon source with the energy is used and be thoroughly mineralized into H
2o and CO
2.
Compared with prior art, tool of the present invention has the following advantages:
The present invention is for the bacterial strain of the p-Xylol of degrading, called after Pan Duola bacterium (Pandoraea sp.) WL1, preserving number is CGMCC NO.7897, this bacterial strain is aerobic type Gram-negative bacterium, there is the efficient degradation ability of p-Xylol, can be take p-Xylol as carbon source and the energy is grown and degradable different starting point concentration substrates; This bacterial strain can be with common metabolic way degrade p-Xylol and toluene simultaneously; The present invention is that biological process processing is laid a good foundation containing the industrial application of the organic exhaust gas of p-Xylol.
In the prior art, at home and abroad there is no so far the report that Pandoraea belongs to strains for degrading p-Xylol.
Accompanying drawing explanation
Fig. 1 is the transmission electron microscope photo of Pan Duola bacterium (Pandoraea sp.) WL1;
Fig. 2 is the 16s rRNA gene fragment Phylogenetic Relationships of Pan Duola bacterium (Pandoraea sp.) WL1;
Fig. 3 is the characteristic of Pan Duola bacterium under differing temps (Pandoraea sp.) WL1 degraded p-Xylol;
Fig. 4 is the characteristic of Pan Duola bacterium (Pandoraea sp.) WL1 degraded p-Xylol under different pH values;
Fig. 5 is the characteristic of different starting point concentration Pan Duola bacterium (Pandoraea sp.) WL1 degraded p-Xylol;
Fig. 6 is simultaneously the degrade characteristic of p-Xylol, toluene of Pan Duola bacterium (Pandoraea sp.) WL1;
Fig. 7 is that Pan Duola bacterium (Pandoraea sp.) WL1 is seeded to the simulation organic exhaust gas of bio-trickling device processing containing p-Xylol.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited to this:
Embodiment 1: the separation of bacterial strain and evaluation:
1. the domestication breeding of bacterial strain:
The active sludge of taking from pharmaceutical factory (in March, 2011, Dongyang City Zhejiang, the Zhejiang Province abundant Biology Pharmacy Co., Ltd of Pu Luokang) Sewage outlet is passed into target contaminant (p-Xylol) and carry out aeration domestication stable rear (at least 1 month), and in constant-temperature table, utilize 300mL culturing bottle, add after 1mL active sludge, 50mL minimal medium and 10～20 μ L target contaminants (p-Xylol), some cycles of enrichment (3～5 times), the microflora of acquisition degradable target contaminant (p-Xylol); Utilize R2A solid medium (10 times method of dilution butteron on plate, method of scoring) to carry out the purifies and separates of degradable bacterial strain, be about to microflora and by 10 times of dilution methods, carry out serial dilution with aseptic deionized water, get 10
-4to 10
-8the bacterium liquid of extension rate is coated the flat board of R2A solid medium, be inverted in constant incubator, cultivate 2～3 days for 30 ℃, picking list bacterium colony is within containing the culturing bottle of minimal medium, add target contaminant (p-Xylol) to investigate its degradation effect, to there is again efficient degradation effect bacterium liquid and carry out purifying again, so repeat some all after dates (3～5 times), according to the diversity judgement of the growing state of bacterium colony on flat board, apparent characteristic, obtain the pure bacterial strain that a strain degradation effect is good, for the bacterial strain of the p-Xylol of degrading.This bacterial strain called after Pan Duola bacterium (Pandoraea sp.) WL1.The short term storage of this bacterial strain, access R2A solid medium (slant medium), in 4 ℃ of preservations of refrigerator.Be preserved in for a long time China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date: on 07 08th, 2013, deposit number: CGMCC NO.7897.。
2. culture condition:
In minimal medium 1L, inorganic salt are cultivated and are comprised of the component of following weight:
2.5g (NH
4)
2sO
4, 0.1g MgCl
26H
2o, 0.01g EDTA, 0.002g ZnSO
47H
2o, 0.001g CaCl
22H
2o, 0.005g FeSO
47H
2o, 0.0002g Na
2moO
42H
2o, 0.0002g CuSO
45H
2o, 0.0004g CoCl
26H
2o, 0.001g MnCl
24H
2o, 1.6g K
2hPO
4, 0.8g NaH
2pO
42H
2the deionized water of O and surplus.
In R2A solid medium 1L, described R2A solid medium is comprised of the component of following weight:
0.5g yeast powder, 0.5g Tryptones, 0.5g casamino acids, 0.5g glucose, 0.5g Zulkovsky starch, 0.3g K
2hPO
4, 0.05g MgSO
47H
2the deionized water of O, 0.3g Sodium.alpha.-ketopropionate, 15.0g agar and surplus; Stirring and dissolving, adjusts after pH to 7.2 121 ℃ of sterilizing 15min.
Culture condition: suitable growth pH scope is 6.0～7.5; Suitable growth temperature is 25～35 ℃.
3. strain morphology and molecular biology identification:
This Pseudomonas belongs in Pandoraea, and bacterium colony is roundlet shape, more transparent, and the smooth of the edge is neat, diameter 0.2mm left and right; As shown in Figure 1, somatic cells is elongated rod shape, 2.0 μ m left and right, and the raw flagellum of end, has folder film, without gemma; For aerobic type Gram-negative bacteria.
The bacterial strain for the p-Xylol of degrading obtaining records the known sequence of its 16S rRNA gene order and GenBank and carries out sequence analysis, finds that multiple bacterial strain sequence similarities that it and Pandoraea belong to all reach more than 98%.Choose the gene order of some bacterial strains and utilize Mega5.0 software that these corresponding sequence and this bacterium sequence are carried out to homology comparison, set up systematic evolution tree (as Fig. 2), and upload gene order to Genbank, obtain gene order number (KF233594); By constructing system, grow relation, determine that the sibship of this bacterial strain and Pandoraea pnomenusa is nearest, similarity is greater than 99%; Therefore, this bacterial strain is attributed to Pandoraea and belongs to, and called after Pan Duola bacterium (Pandoraea sp.) WL1.
Embodiment 2: the degradation characteristic of Pan Duola bacterium (Pandoraea sp.) WL1
1. the degradation characteristic of Pan Duola bacterium (Pandoraea sp.) WL1 under condition of different temperatures (25～35 ℃):
The sole carbon source of (Pandoraea sp.) WL1 using p-Xylol as Pan Duola bacterium, inoculation 3mL logarithmic phase bacteria suspension (OD
600nm=0.235) in 100mL minimal medium (initial pH=6.5) and be placed in 630mL sealing culturing bottle (designing respectively 2 Duplicate Samples and 1 blank group under each temperature condition), adding p-Xylol to make starting point concentration in system is 41.0mg/L, be placed in respectively 25,30,35 ℃, cultured continuously in 150r/min constant-temperature table, interval certain hour sampling analysis (result is as Fig. 3).The present embodiment explanation, when higher p-Xylol starting point concentration (41.0mg/L), when degradation characteristic when temperature is 35 ℃ is obviously better than 25 ℃ and 30 ℃; Wherein, 25 ℃ are most disadvantageous in the degraded of Pan Duola bacterium (Pandoraea sp.) WL1, and 30～35 ℃ is its optimum growth temperature; Result shows, the degraded p-Xylol that Pan Duola bacterium in differing temps environment (Pandoraea sp.) WL1 all can be in various degree.
2. the degradation characteristic of Pan Duola bacterium (Pandoraea sp.) WL1 under condition of different pH (4～8):
The pH that seals the 100mL minimal medium in culturing bottle (designing 2 Duplicate Samples and 1 blank group under each condition) with the 1mol/L NaOH aqueous solution or 1mol/L HCl adjusting 630mL is respectively 4,5,6,6.5,7,8; Under the condition that is 41.0mg/L in initial p-Xylol concentration, add 3mL logarithmic phase bacteria suspension (OD
600nm=0.170), be placed in respectively 35 ℃, the interior cultured continuously of 150r/min constant-temperature table, interval certain hour sampling analysis (result is as Fig. 4).Result shows, in pH value, is 6～7 o'clock, and in the progressively degradation process of p-Xylol, its degradation characteristic is obviously better than other pH values; Too high or too low (be less than 4 or be greater than 8) of pH value, all can affect the degradation process (degradation of substrates is incomplete) of this bacterial strain, and pH value is 6～7th, its suitableeest degraded scope; Pan Duola bacterium in different pH environment (Pandoraea sp.) WL1 all can be in various degree degraded p-Xylol, for its application in different pH environment provides assurance.
3. the degradation characteristic of the Pan Duola bacterium under different starting point concentrations (Pandoraea sp.) WL1:
The sole carbon source of (Pandoraea sp.) WL1 using p-Xylol as Pan Duola bacterium, inoculation 3mL logarithmic phase bacteria suspension (OD
600nm=0.082) in 100mL minimal medium (initial pH=6.5) and be placed in 630mL sealing culturing bottle (designing respectively 2 Duplicate Samples and 1 blank group under each starting point concentration), add p-Xylol to make starting point concentration in system be respectively 6.9mg/L, 13.7mg/L, 27.6mg/L, 41.0mg/L, 54.7mg/L, 67.8mg/L, be placed in respectively 35 ℃, the interior cultured continuously of 150r/min constant-temperature table, interval certain hour sampling analysis (result is as Fig. 5).Result shows, Pan Duola bacterium (Pandoraea sp.) WL1 can be respectively at 14～90h p-Xylol of different starting point concentrations of degrading, and there is the degradation capability of efficient stable.
4. simultaneously degrade p-Xylol, toluene of Pan Duola bacterium (Pandoraea sp.) WL1:
Using p-Xylol, toluene, as Pan Duola bacterium (Pandoraea sp.) WL1 as the same 630mL of carbon source, seal (designing 2 Duplicate Samples and 1 blank group) in culturing bottle simultaneously and investigate its degradation characteristic, inoculation 3mL logarithmic phase bacteria suspension (OD
600nm=0.149) to 100mL minimal medium (initial pH=6.5), add p-Xylol and toluene, make its starting point concentration be respectively 13.89mg/L, 14.03mg/L, be placed in respectively 35 ℃, the interior cultured continuously of 150r/min constant-temperature table, interval certain hour sampling analysis (result is as Fig. 6).Result shows, Pan Duola bacterium (Pandoraea sp.) WL1 is the p-Xylol in main degraded system in 24h, and degradation of toluene amount is less, but toluene is mainly degraded in later stage 12h, in 36h, p-Xylol, toluene is almost degradable, illustrate that this bacterial strain has the wide in range property of good substrate.
Embodiment 3: Pan Duola bacterium (Pandoraea sp.) WL1 is seeded to bio-trickling device and processes containing p-Xylol simulation organic exhaust gas
Adopting application number is the disclosed bio-trickling device of 201210019232.2 Chinese patent application and method, Pan Duola bacterium (Pandoraea sp.) WL1 is seeded to the processing that contains p-Xylol organic exhaust gas in bio-trickling device, through biofilm, tame to the steady stage, the i.e. preparation simulation organic exhaust gas take p-Xylol as target contaminant, Pan Duola bacterium (Pandoraea sp.) WL1 bacterial suspension inoculation to bio-trickling device is tamed to steady running through biofilm, contain p-Xylol simulation VOCs treatment (result is as Fig. 7).Result shows, this bio-trickling device, through the biofilm domestication process of about 14 days, reaches stable operation stage.In biofilm domestication early stage (1～10 day), inlet gas concentration remains on 200～800mg/m
3in scope, the removal efficiency of p-Xylol unstable (50%～80%); Enter into the biofilm domestication later stage in stage, maintain its inlet gas concentration at 800～1000mg/m
3in scope, removal efficiency rises to 90% left and right gradually by 70%, and keeps the p-Xylol removal effect of stability and high efficiency; Steady stage in later stage (18～24 days) is also investigated the different residence time, and (20s, 40s, 60s, 80s, 100s, corresponding air input is respectively 1.98m
3/ h, 0.99m
3/ h, 0.66m
3/ h, 0.50m
3/ h, 0.40m
3/ p-Xylol removal effect h).Above data absolutely prove, the bio-trickling device of inoculation Pan Duola bacterium (Pandoraea sp.) WL1 can reach steady running through the short-term biofilm domestication of approximately 14 days, and obtained the good p-Xylol that contains and simulated voc_s removal effect, there is good prospects for commercial application.
Claims (9)
1. for a bacterial strain for the p-Xylol of degrading, it is characterized in that, called after Pan Duola bacterium (Pandoraea sp.) WL1, preserving number is CGMCC NO.7897.
2. a cultural method for the bacterial strain for the p-Xylol of degrading as claimed in claim 1, is characterized in that, comprises the following steps:
1) get the active sludge of pharmaceutical factory's Sewage outlet, pass into p-Xylol and carry out aeration domestication, active sludge, minimal medium and p-Xylol are joined in culturing bottle after stable, in shaking table, cultivate, obtain microflora;
2) utilize R2A solid medium in conjunction with method of dilution butteron on plate and method of scoring, to carry out the purifies and separates of aimed strain, obtain the bacterial strain for the p-Xylol of degrading.
3. the cultural method of the bacterial strain for the p-Xylol of degrading as claimed in claim 2, is characterized in that, the volume ratio of described active sludge, minimal medium and p-Xylol is 1:30～70:0.005～0.05.
4. the cultural method of the bacterial strain for the p-Xylol of degrading as claimed in claim 3, is characterized in that, the volume ratio of described active sludge, minimal medium and p-Xylol is 1:50:0.01～0.02.
5. the cultural method of the bacterial strain for the p-Xylol of degrading as claimed in claim 2, is characterized in that, in minimal medium 1L, described minimal medium is comprised of the component of following weight:
6. the cultural method of the bacterial strain for the p-Xylol of degrading as claimed in claim 2, is characterized in that, in R2A solid medium 1L, described R2A solid medium is comprised of the component of following weight:
7. the bacterial strain for the p-Xylol of degrading as claimed in claim 1 is in the application of processing containing p-Xylol organic exhaust gas.
8. application as claimed in claim 7, is characterized in that, the inoculation for the p-Xylol of degrading claimed in claim 1, to the VOCs treatment that contains p-Xylol in bio-trickling device, is tamed to the steady stage through biofilm.
9. application as claimed in claim 8, is characterized in that, degraded p-Xylol carries out under 25～35 ℃, pH=4～8 condition.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195810A (en) * | 2007-11-30 | 2008-06-11 | 华南理工大学 | High-efficiency degradation bacterium series of polycyclic aromatic hydrocarbon and uses thereof |
| KR20100053745A (en) * | 2008-11-13 | 2010-05-24 | 충북대학교 산학협력단 | Bacterial strains promoting plant growth by solubilizing rock phosphate and method for promoting plant growth using the same |
| CN102080055A (en) * | 2009-11-26 | 2011-06-01 | 北京师范大学 | Indenopyrene degradation bacterium and application thereof |
| CN102093973A (en) * | 2010-12-08 | 2011-06-15 | 中南大学 | Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application |
| CN102533586A (en) * | 2011-11-18 | 2012-07-04 | 浙江工业大学 | Pandora bacterium with dichloromethane degrading capability and application thereof |
| CN102533682A (en) * | 2011-12-23 | 2012-07-04 | 北京师范大学 | Enzyme capable of catalyzing and degrading indeno[1, 2, 3-cd]pyrene |
| CN102559542A (en) * | 2011-12-14 | 2012-07-11 | 首创爱华(天津)市政环境工程有限公司 | Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method |
-
2013
- 2013-11-26 CN CN201310609086.3A patent/CN103756928B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195810A (en) * | 2007-11-30 | 2008-06-11 | 华南理工大学 | High-efficiency degradation bacterium series of polycyclic aromatic hydrocarbon and uses thereof |
| KR20100053745A (en) * | 2008-11-13 | 2010-05-24 | 충북대학교 산학협력단 | Bacterial strains promoting plant growth by solubilizing rock phosphate and method for promoting plant growth using the same |
| CN102080055A (en) * | 2009-11-26 | 2011-06-01 | 北京师范大学 | Indenopyrene degradation bacterium and application thereof |
| CN102093973A (en) * | 2010-12-08 | 2011-06-15 | 中南大学 | Bacterial strain Pandoraea sp. B-6 for processing high-density papermaking black liquor and application |
| CN102533586A (en) * | 2011-11-18 | 2012-07-04 | 浙江工业大学 | Pandora bacterium with dichloromethane degrading capability and application thereof |
| CN102559542A (en) * | 2011-12-14 | 2012-07-11 | 首创爱华(天津)市政环境工程有限公司 | Strain Pandoraea sp. for removing ammonia nitrogen in sewage under low-temperature condition and isolated culture method |
| CN102533682A (en) * | 2011-12-23 | 2012-07-04 | 北京师范大学 | Enzyme capable of catalyzing and degrading indeno[1, 2, 3-cd]pyrene |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475144A (en) * | 2017-04-11 | 2017-12-15 | 辽宁科技大学 | A kind of Pandora bacterium and its application method |
| CN107674844A (en) * | 2017-08-15 | 2018-02-09 | 云南万魁生物科技有限公司 | A kind of new Methylobacterium MR1 and its application |
| CN107674844B (en) * | 2017-08-15 | 2020-09-25 | 云南万魁生物科技有限公司 | Novel methylobacterium MR1 and application thereof |
| CN108715820A (en) * | 2018-05-30 | 2018-10-30 | 水云天（天津）生物科技发展有限公司 | A method of the composite bacteria agent and utilization composite bacteria agent degradation VOCs of degradation VOCs |
| CN110229766A (en) * | 2019-06-14 | 2019-09-13 | 浙江工业大学 | Aoxidize microbacterium and its application in degradable organic pollutant |
| CN110229766B (en) * | 2019-06-14 | 2021-06-08 | 浙江工业大学 | Microbacterium oxydans and application thereof in degradation of organic pollutants |
| CN110964660A (en) * | 2019-10-10 | 2020-04-07 | 郑州轻工业学院 | Arthrobacter for degrading chlorobenzene pollutants as well as culture method and application thereof |
| CN110964660B (en) * | 2019-10-10 | 2022-10-04 | 郑州轻工业大学 | Arthrobacter for degrading chlorobenzene pollutants as well as culture method and application thereof |
| CN112899192A (en) * | 2021-02-04 | 2021-06-04 | 华东理工大学 | BTEX degrading bacterium and screening method and application thereof |
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