CN104623154A - Chinese herbal medicine composition for treating alzheimer's disease and preparation method thereof - Google Patents
Chinese herbal medicine composition for treating alzheimer's disease and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine composition for treating alzheimer's disease, which comprises the following components in parts by mass: 8-28 parts of rhizoma acori graminei, 8-28 parts of radix rehmanniae praeparata, 8-28 parts of pulp of cornus, 5-25 parts of wolfberry, 2-22 parts of salviae miltiorrhizae, 2-22 parts of polygala amflra, 5-25 parts of astragalus membranaceus, 2-22 parts of Ligusticum wallichii, 5-25 parts of angelica sinensis, 2-22 parts of poria cocos, 1-20 parts of pinellia ternate and 1-20 parts of radix glycyrrhizae. The invention also discloses a preparation method of the Chinese herbal medicine composition for treating alzheimer's disease. The Chinese herbal medicine composition is implemented based on kidney tonifying in a way of tonifying and nursing rather than only tonifying, so that the nourishment of essences is active, and the essences can be transmitted and distributed for nourishing the whole body, thereby effectively achieving the effect of treating root causes.
Description
Technical field
The invention belongs to technical field of pharmaceuticals, be specifically related to a kind of herbal composite for the treatment of senile dementia, the present invention also provides a kind of preparation method for the treatment of the herbal composite of senile dementia.
Background technology
Brain being the marrow sea, alzheimer disease is exactly mainly brains deficiency the traditional Chinese medical science, is not enough to nourish brain key, therefore there will be forgetful, the symptom such as be slow in action.The raw marrow of essence, that mends marrow is essence foot at all.And the generation of essence is mainly at kidney, therefore kidney-tonifying sperm-generating is significant when treating primary disease, old people's deficient qi and blood, therefore needs invigorating QI and blood.Deficient qi and blood then QI-blood circulation is unable, and easy concurrent expectorant, the stasis of blood etc., for a long time then functional activity of QI being not smooth, meridians blood vessels run retardance.Therefore need with invigorating blood circulation, regulating QI, reduce phlegm, the method such as to have one's ideas straightened out, QI and blood is followed work smooth, whole body all can be nourished; The medicine of current clinical treatment is based on Western medicine (lecithin, trifluoperazine etc.), and take effect slow, and may have certain side effect to Liver and kidney etc., effect is unsatisfactory.
Summary of the invention
The object of this invention is to provide a kind of herbal composite for the treatment of senile dementia, under the guidance of theory of Chinese medical science, adopt dialectical treating, treatment must aim at the pathogenesis of disease, the method for the treatment of both the principal and secondary aspects of a disease, solve the Chinese medicine that exists in prior art or Western medicine curative effect is aobvious, side effect is large problem, drug regimen providing a kind of good effect, side effect little and preparation method thereof.Medicine medicine source of the present invention is wide, is convenient to obtain, cheap, is applicable to clinical expansion and uses.
First technical scheme of the present invention is, treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae.
In technique scheme,
Further, this herbal composite comprises following component according to mass parts: Rhizoma Acori Graminei 15 parts-20 parts, Radix Rehmanniae Preparata 15 parts-20 parts, Fructus Corni 15 parts-20 parts, Fructus Lycii 12 parts-18 parts, Radix Salviae Miltiorrhizae 10 parts-15 parts, Radix Polygalae 10 parts-15 parts, the Radix Astragali 12 parts-18 parts, Rhizoma Chuanxiong 10 parts-15 parts, Radix Angelicae Sinensis 12 parts-18 parts, 10 parts-15 parts, Poria, the Rhizoma Pinelliae 8 parts-12 parts, 8 parts-12 parts, Radix Glycyrrhizae.
Further, this herbal composite comprises following component according to mass parts: Rhizoma Acori Graminei 18 parts, Radix Rehmanniae Preparata 18 parts, Fructus Corni 18 parts, Fructus Lycii 15 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 15 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 15 parts, 12 parts, Poria, the Rhizoma Pinelliae 10 parts, 10 parts, Radix Glycyrrhizae.
This herbal composite is prepared into capsule, pill, tablet, granule or water decoction.
Second technical scheme of the present invention is, a kind of preparation method for the treatment of the herbal composite of senile dementia is specifically implemented according to following steps:
Step 1, weighing, weigh following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae;
Step 2, prepare volatile oil, load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 6-10 times of water gaging and keep boiling 6-8h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use;
Step 3, by the respectively 6-10 times of soak by water 2 times of the medicinal residues in load weighted Radix Rehmanniae Preparata 8, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2, time is respectively 2-3h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated;
Step 4, concentrated solution added ethanol leaves standstill, cold preservation 12-36 hour, it is 1.15-1.35 that decompression recycling ethanol concentrates density, makes extractum; Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite for the treatment of senile dementia.
In technique scheme,
The concentration of ethanol is 50%-95%.
This preparation method prepares herbal composite and is prepared into capsule, pill, tablet, granule or water decoction.
The invention has the beneficial effects as follows: this herbal composite, based on the kidney invigorating, does not stick to merely tonic, but pay attention to " benefits ", " tune " is double executes, and marrow is given birth to and brought up active, and can effective defeated cloth nourishing whole body, effectively accomplish treatment must aim at the pathogenesis of disease.
Accompanying drawing explanation
Fig. 1 is the route map that the present invention six groups of rats find platform, wherein, figure a represents Normal group, figure b represents model group, figure c represents low dose group, dosage group during figure d represents, figure e represents high dose group, figure f represents piracetam matched group, I, II, III and IV represent first quartile, the second quadrant, third quadrant and fourth quadrant respectively, fourth quadrant black box represents the initiation site of rat motor, and the second quadrant black box represents the termination site of rat motor, and in the second quadrant, platform is hidden in circle representative; The figure a-curve of scheming in f represents the movement locus of rat.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in detail.
The invention provides a kind of herbal composite for the treatment of senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae.
Further, said composition comprises following component according to mass parts: Rhizoma Acori Graminei 15 parts-20 parts, Radix Rehmanniae Preparata 15 parts-20 parts, Fructus Corni 15 parts-20 parts, Fructus Lycii 12 parts-18 parts, Radix Salviae Miltiorrhizae 10 parts-15 parts, Radix Polygalae 10 parts-15 parts, the Radix Astragali 12 parts-18 parts, Rhizoma Chuanxiong 10 parts-15 parts, Radix Angelicae Sinensis 12 parts-18 parts, 10 parts-15 parts, Poria, the Rhizoma Pinelliae 8 parts-12 parts, 8 parts-12 parts, Radix Glycyrrhizae.
Further, said composition comprises following component according to mass parts: Rhizoma Acori Graminei 18 parts, Radix Rehmanniae Preparata 18 parts, Fructus Corni 18 parts, Fructus Lycii 15 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 15 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 15 parts, 12 parts, Poria, the Rhizoma Pinelliae 10 parts, 10 parts, Radix Glycyrrhizae.
Wherein, Rhizoma Acori Graminei: resuscitation inducing and mind tranquilizing, removing dampness to restore normal function of the stomach; Radix Rehmanniae Preparata: enrich blood, YIN nourishing, beneficial essence fills out marrow; Fructus Corni: liver and kidney tonifying, restrains astringent or styptic treatment for spontaneous sweating; Fructus Lycii: promoting blood flow to regulate menstruation, removing heat from blood, eliminating carbuncle, clearing away heart-fire for tranquillization; Radix Salviae Miltiorrhizae: promoting blood flow to regulate menstruation, removing heat from blood, eliminating carbuncle, clearing away heart-fire for tranquillization; Radix Polygalae: mind tranquilizing and the heart calming, eliminate the phlegm have one's ideas straightened out, dissipation carbuncle; The Radix Astragali: tonifying Qi and lifting yang, stomach reinforcing consolidating superficial resistance, inducing diuresis to remove edema, detoxification granulation promoting; Rhizoma Chuanxiong: blood-activating and qi-promoting, wind-expelling pain-stopping; Radix Angelicae Sinensis: enrich blood, invigorates blood circulation, regulating menstruation, pain relieving, intestine moistening; Poria: removing toxic substances dehumidifying, easing joint movement; The Rhizoma Pinelliae: drying dampness to eliminate phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, dissolving lump and resolving mass; Radix Glycyrrhizae: QI invigorating invigorating middle warmer, expelling phlegm for arresting cough, relieving spasm to stop pain, the mediation property of medicine, heat-clearing and toxic substances removing.
Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii kidney tonifying, essence replenishing, essence can give birth to marrow, takes a tonic or nourishing food to build up one's health brains; The merit of air making-up and spleen enlivening played altogether by the Radix Astragali, Poria; Radix Salviae Miltiorrhizae blood circulation promoting and blood stasis dispelling, Rhizoma Chuanxiong promoting flow of QI and blood, Radix Angelicae Sinensis blood circulation promoting and enriching, blood stasis in the popular smooth and easy then change brain of QI and blood; Rhizoma Acori Graminei is had one's ideas straightened out, Radix Polygalae mind calming, the Rhizoma Pinelliae reduce phlegm, all can inducing resuscitation; Radix Glycyrrhizae QI invigorating, holds concurrently with coordinating the actions of various ingredients in a prescription.
The present invention also provides a kind of preparation method for the treatment of the herbal composite of senile dementia, specifically implements according to following steps:
Step 1, weighing, weigh following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae;
Step 2, prepare volatile oil, load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 6-10 times of water gaging and keep boiling 6-8h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use;
Step 3, by the respectively 6-10 times of soak by water 2 times of the medicinal residues in load weighted Radix Rehmanniae Preparata 8, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2, time is respectively 2-3h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated;
Step 4, concentrated solution to be added concentration be that the ethanol of 50%-95% leaves standstill, cold preservation 12-36 hour, and it is between 1.15-1.35 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite for the treatment of senile dementia.
Technique makes suitable capsule, pill, tablet, granule etc. routinely; All can also adopt water decoction by decoction pieces.
Rhizoma Acori Graminei, containing volatile oil, has maincenter sedation, can secrete by facilitating digestion liquid, prevents the abnormal fermentation of gastrointestinal, can strengthen learning memory.Radix Angelicae Sinensis is containing volatile oil, and contained ferulic acid can improve peripheral circulation, reduces blood pressure, and has certain antioxidation and effect of scavenging radical, has obvious inhibitory action to cerebral anoxia.Rhizoma Chuanxiong is containing multiple volatile oil, and energy coronary artery dilator, improves microcirculation, antithrombotic effect.
Clinical case: patient, female, 68 years old, palpitation and insomnia over nearly five months, bradykinesia, is slow in action, and lassitude is with cognitive disorder in various degree, memory is faded to memory loss, take each 3 of medicine capsule of the present invention, every day 3 times, after 3 weeks, insomnia is lost memory and is taken a turn for the better, continue to take January, the mental status of patient, language, action return to normal condition.
Patient, man, 73 years old, occurs having a dull expression on one's face, language is disorder nearly April, memory and judgement obviously go down, and namely what someone said is forgotten in a twinkle, and weak happiness is sighed, diet is indigestion and loss of appetite, takes each 3 of medicine capsule of the present invention, every day 3 times, after 2 weeks, patient's mental status is clearly better, and speech is normal, and judgment improves, diet increases, and the February patient's language of continuing to take medicine is fluent, and memory significantly improves, diet recovers normal, and the private prosecution mental status is good, effectively conscious.
Patient, man, 75 years old, nearly 7 months start to occur emotional lability, being happy and angry uncertainly, insomnia, belch, anorexia, gos out and once repeatedly gets lost, take medicine water decoction of the present invention every day 1 dose, every day 2 times, emotion is uncertain after totally 20 doses, insomnia is alleviated, and diet takes a turn for the better, energetic after February of continuing to take medicine, exchange unobstructed with people, memory is clearly better.
Embodiment 1
Treat a herbal composite for senile dementia, comprise following component: Rhizoma Acori Graminei 18 grams, Radix Rehmanniae Preparata 18 grams, Fructus Corni 18 grams, Fructus Lycii 15 grams, Radix Salviae Miltiorrhizae 12 grams, Radix Polygalae 12 grams, the Radix Astragali 15 grams, Rhizoma Chuanxiong 12 grams, Radix Angelicae Sinensis 15 grams, 12 grams, Poria, the Rhizoma Pinelliae 10 grams, 10 grams, Radix Glycyrrhizae.
Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort decoction pieces are added 6 times of water gagings and keep boiling 6h, and steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and decoction pieces aqueous solution for subsequent use; All the other medical materials and medicinal residues are added 8 times of soak by water 2 times respectively, time is respectively 2h, 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution is merged, and adds 80% ethanol and leave standstill cold preservation 12 hours after concentrated, it is 1.25 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, is finally processed into acceptable capsule clinically.
Embodiment 2
Treat a herbal composite for senile dementia, comprise following component: Rhizoma Acori Graminei 15 parts, Radix Rehmanniae Preparata 12 parts, Fructus Corni 9 parts, Fructus Lycii 9 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 15 parts, Rhizoma Chuanxiong 9 parts, Radix Angelicae Sinensis 15 parts, 15 parts, Poria, the Rhizoma Pinelliae 9 parts, 9 parts, Radix Glycyrrhizae.
Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort decoction pieces are added 10 times of water gagings and keep boiling 7h, and steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and decoction pieces aqueous solution for subsequent use; All the other medical materials and medicinal residues are added 10 times of soak by water 2 times respectively, time is respectively 3h, 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution is merged, and adds 85% ethanol and leave standstill cold preservation 36 hours after concentrated, it is between 1.33 that decompression recycling ethanol concentrates density, makes extractum.Extract powder and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, is finally processed into acceptable pill clinically.
Embodiment 3
Treat a herbal composite for senile dementia, comprise following component: Rhizoma Acori Graminei 6 parts, Radix Rehmanniae Preparata 9 parts, Fructus Corni 12 parts, Fructus Lycii 9 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 6 parts, Rhizoma Chuanxiong 15 parts, Radix Angelicae Sinensis 12 parts, 9 parts, Poria, the Rhizoma Pinelliae 10 parts, 3 parts, Radix Glycyrrhizae.Be decocted in water for oral dose, every day 1 dose, every day 2 times.
Embodiment 4
Treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 8 parts, Radix Rehmanniae Preparata 28 parts, Fructus Corni 8 parts, Fructus Lycii 25 parts, Radix Salviae Miltiorrhizae 2 parts, Radix Polygalae 22 parts, the Radix Astragali 5 parts, Rhizoma Chuanxiong 22 parts, Radix Angelicae Sinensis 5 parts, 22 parts, Poria, the Rhizoma Pinelliae 1 part, 20 parts, Radix Glycyrrhizae.
Said components is weighed: Rhizoma Acori Graminei 8 parts, Radix Rehmanniae Preparata 28 parts, Fructus Corni 8 parts, Fructus Lycii 25 parts, Radix Salviae Miltiorrhizae 2 parts, Radix Polygalae 22 parts, the Radix Astragali 5 parts, Rhizoma Chuanxiong 22 parts, Radix Angelicae Sinensis 5 parts, 22 parts, Poria, the Rhizoma Pinelliae 1 part, 20 parts, Radix Glycyrrhizae according to mass parts; Load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 6 times of water gagings and keep boiling 8h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use; Load weighted Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and above-mentioned medicinal residues are distinguished 10 times of soak by water 2 times, and the time is respectively 2h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated; Concentrated solution is added concentration be 50% ethanol leave standstill, cold preservation 36 hours, it is 1.15 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite capsule for the treatment of senile dementia.
Embodiment 5
Treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 28 parts, Radix Rehmanniae Preparata 8 parts, Fructus Corni 28 parts, Fructus Lycii 5 parts, Radix Salviae Miltiorrhizae 22 parts, Radix Polygalae 2 parts, the Radix Astragali 25 parts, Rhizoma Chuanxiong 2 parts, Radix Angelicae Sinensis 25 parts, 2 parts, Poria, the Rhizoma Pinelliae 20 parts, 1 part, Radix Glycyrrhizae.
Said components is weighed: Rhizoma Acori Graminei 28 parts, Radix Rehmanniae Preparata 8 parts, Fructus Corni 28 parts, Fructus Lycii 5 parts, Radix Salviae Miltiorrhizae 22 parts, Radix Polygalae 2 parts, the Radix Astragali 25 parts, Rhizoma Chuanxiong 2 parts, Radix Angelicae Sinensis 25 parts, 2 parts, Poria, the Rhizoma Pinelliae 20 parts, 1 part, Radix Glycyrrhizae according to mass parts; Load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 10 times of water gagings and keep boiling 6h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use; Load weighted Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and above-mentioned medicinal residues are distinguished 6 times of soak by water 2 times, and the time is respectively 3h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated; Concentrated solution is added concentration be 95% ethanol leave standstill, cold preservation 12-36 hour, it is 1.35 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite pill for the treatment of senile dementia.
Embodiment 6
Treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 15 parts, Radix Rehmanniae Preparata 20 parts, Fructus Corni 15 parts, Fructus Lycii 18 parts, Radix Salviae Miltiorrhizae 10 parts, Radix Polygalae 15 parts, the Radix Astragali 12 parts, Rhizoma Chuanxiong 15 parts, Radix Angelicae Sinensis 12 parts, 15 parts, Poria, the Rhizoma Pinelliae 8 parts, 12 parts, Radix Glycyrrhizae;
Said components is weighed: Rhizoma Acori Graminei 15 parts, Radix Rehmanniae Preparata 20 parts, Fructus Corni 15 parts, Fructus Lycii 18 parts, Radix Salviae Miltiorrhizae 10 parts, Radix Polygalae 15 parts, the Radix Astragali 12 parts, Rhizoma Chuanxiong 15 parts, Radix Angelicae Sinensis 12 parts, 15 parts, Poria, the Rhizoma Pinelliae 8 parts, 12 parts, Radix Glycyrrhizae according to mass parts; Load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 8 times of water gagings and keep boiling 7h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use; Medicinal residues in load weighted Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2 are distinguished 8 times of soak by water 2 times, time is respectively 2.5h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated; Concentrated solution is added concentration be 60% ethanol leave standstill, cold preservation 24 hours, it is between 1.25 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite tablet for the treatment of senile dementia.
Embodiment 7
Treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 20 parts, Radix Rehmanniae Preparata 15 parts, Fructus Corni 20 parts, Fructus Lycii 12 parts, Radix Salviae Miltiorrhizae 15 parts, Radix Polygalae 10 parts, the Radix Astragali 18 parts, Rhizoma Chuanxiong 10 parts, Radix Angelicae Sinensis 18 parts, 10 parts, Poria, the Rhizoma Pinelliae 12 parts, 8 parts, Radix Glycyrrhizae;
Said components is weighed: Rhizoma Acori Graminei 20 parts, Radix Rehmanniae Preparata 15 parts, Fructus Corni 20 parts, Fructus Lycii 12 parts, Radix Salviae Miltiorrhizae 15 parts, Radix Polygalae 10 parts, the Radix Astragali 18 parts, Rhizoma Chuanxiong 10 parts, Radix Angelicae Sinensis 18 parts, 10 parts, Poria, the Rhizoma Pinelliae 12 parts, 8 parts, Radix Glycyrrhizae according to mass parts; Load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 7.5 times of water gagings and keep boiling 6.5h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use; Medicinal residues in load weighted Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2 are distinguished 8.5 times of soak by water 2 times, time is respectively 2.4h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated; Concentrated solution is added concentration be 80% ethanol leave standstill, cold preservation 30 hours, it is between 1.30 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite granule for the treatment of senile dementia.
Embodiment 8
Treat a herbal composite for senile dementia, comprise following component according to mass parts: Rhizoma Acori Graminei 18 parts, Radix Rehmanniae Preparata 18 parts, Fructus Corni 18 parts, Fructus Lycii 15 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 15 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 15 parts, 12 parts, Poria, the Rhizoma Pinelliae 10 parts, 10 parts, Radix Glycyrrhizae
Said components is weighed: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae according to mass parts; Load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 6-10 times of water gaging and keep boiling 6-8h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use; Medicinal residues in load weighted Radix Rehmanniae Preparata, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2 are distinguished 6-10 times of soak by water 2 times, time is respectively 2-3h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated; Concentrated solution is added concentration be 50%-95% ethanol leave standstill, cold preservation 12-36 hour, it is between 1.15-1.35 that decompression recycling ethanol concentrates density, makes extractum.Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite for the treatment of senile dementia.
The proved beneficial effect of the invention is carried out below by concrete experimentation:
One, herbal composite of the present invention is to the improvement result of Senile Dementia Model Rats learning memory disorder
1 experiment material
1.1 laboratory animals are grown up SPF level SD rat 60, and male and female half and half, body weight 200 ± 20g, is provided (credit number: SCXK (Henan) 2010-0002) by Henan Province's Experimental Animal Center.
1.2 Experimental agents and reagent D-galactose, Shanghai Chemical Reagent Co., Ltd., Sinopharm Group, lot number 20130916; A β
25-35, Sigma Reagent Company of the U.S., lot number A4559; Piracetam (piracetam), Hubei Huahzong Medicine Co., Ltd, lot number 20130922; Norepinephrine testing cassete, dopamine testing cassete, 5-hydroxy tryptamine testing cassete, choline acetyltransterase test kit and acetylcholine ester enzyme reagent kit, superoxide dismutase testing cassete, malonaldehyde testing cassete, protein quantification testing cassete, all buy and build up Bioengineering Research Institute in Nanjing, lot number is respectively RT131109, RT131129, RT131210, A079-1, A024, A001-3, A003-1, A045-2.
1.3 experimental apparatus stereotaxic instrument, Chengdu Instruement Factory; Morris water maze, Anhui Zhenghua Biology Instrument Equipment Co., Ltd.; Enzyme-linked immunosorbent assay instrument, Bio Rad Laboratories; V-1200 type visible spectrophotometer, Shanghai rib spectrometer instruments and meters company limited; FSH-II type high speed electric refiner, the global scientific instrument factory of Jiangsu Jin Yunshi; SSW type Microcomputerized electric temperature chamber, Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd.; AE-100 type electronic balance, Mettler-Toledo Instrument (Shanghai) Co., Ltd..
2 experimental techniques
2.1 experiment grouping and modeling
Laboratory animal, after standard environment adaptability raises 1 week, is divided into 6 groups: Normal group, model group at random, the basic, normal, high dosage group of medicine of the present invention, piracetam matched group, often organizes 10.Model group, the basic, normal, high dosage group of medicine of the present invention, piracetam matched group: continuous 6 weeks subcutaneous injection D-galactose 150mg/kg/d, within the 7th week, every side Hippocampus injects the A β of 2nmol/L
25-35(with physiological saline solution) be (concrete operations refer to method 2.2) once; Normal group: continuous 6 weeks subcutaneous injection normal saline, within the 7th week, bilateral hippocampus all injects normal saline once.
Injection A β in 2.2 Hippocampus
25-35method
After 10% chloral hydrate intraperitoneal injection of anesthesia rat, be fixed on brain solid positioner, skin of vertex preserved skin, iodophor disinfection.Scalpel median sagittal cuts scalp, peels off periosteum, exposes bregma (Bergam point), with Bergam point for zero, with reference to Paxinos rat brain stereotaxic atlas, by Bergam point 3.2mm backward, the other 2.2mm of center line, cranial drill bores skull, under brain solid positioner guides, in bilateral hippocampus, slowly inject the medicine (bilateral total amount 4 μ l) of 2 μ l with microsyringe respectively, the degree of depth is 4mm, speed is 0.2 μ l/min, let the acupuncture needle remain at a certain point 5min after having injected.Art finishes, the bone wax of the injection orifice on skull is closed, sterilization, skin suture, and give antibiotic treatment for three days on end.
2.3 medicine preparation and medications
2.3.1 Rhizoma Acori Graminei 1.8kg, Radix Rehmanniae Preparata 1.8kg, Fructus Corni 1.8kg, Fructus Lycii 1.5kg, Radix Salviae Miltiorrhizae 1.2kg, Radix Polygalae 1.2kg, Radix Astragali 1.5kg, Rhizoma Chuanxiong 1.2kg, Radix Angelicae Sinensis 1.5kg, Poria 1.2kg, Rhizoma Pinelliae 1.0kg, Radix Glycyrrhizae 1.0kg are got in medicine preparation.Each taste Chinese medicine is added 6 times amount soak by water 2 times, and merge decocting liquid, be mixed with the medicinal liquid of 1g/ml, 2g/ml, 4g/ml respectively, autoclaving, 4 DEG C of Refrigerator stores are for subsequent use.
2.3.2 medication
Medicine basic, normal, high dosage group and piracetam matched group: give 1g/ml, 2g/ml, 4g/ml medicine water extract of the present invention and piracetam (40mg/ml) gavage the 1st day respectively from modeling, each treated animal presses 1ml/100g administration, be 1 time/d, continuous 8 weeks; Normal group and model group give the normal saline gavage of 1ml/100g every day, continuous 8 weeks.Each treated animal records weekly a body weight, adjustment injected dose.
2.4 learning and memory in rats abilities measure-Morris water maze laboratory
Morris water maze to be a diameter be 160cm, be highly the black round pool of 50cm, peripheral pool posts pattern of different colors, different shapes as mark.Depth of water 30cm in pond, adds ink in water, makes water opaque, and water temperature remains on (24 ± 1) DEG C.The hiding platform diameter 14cm of black, is positioned at the second quadrant, and 1cm lower than the water surface.
The test of Morris water maze comprises orientation navigation test and space exploration test.Orientation navigation test duration 5d, every day every animal training 2 times.Rat is put into pond towards pool wall by 4 place of entry, and record rat, from entering water to finding hiding platform required time (i.e. escape latency, EL), detects the acquisition capability of rat learning and memory.The 6d of Morris water maze, carry out removing the space exploration experiment of hiding platform under water, the record rat distance of swimming in the quadrant of former hiding platform place to account in the percentage ratio of total distance, 120s through the route map of search platform in the number of times of platform position and 120s to determine its search strategy.
In 2.5 rat hippocampus, SOD, MDA, NE, DA, 5-HT, ChAT and AChE detect
The hippocampal tissue of taking-up is placed on rinsing in the normal saline of 4 DEG C, removing bloodstain, filter paper is wiped dry, weigh, add 9 times of mL normal saline, use tissue refiner homogenate, centrifuge (3500 turns/min) centrifugal 15min, gets supernatant, obtains 10% tissue homogenate.According to test kit description, Coomassie Brilliant Blue is adopted to measure protein content, hydroxylamine assay measures superoxide dismutase (SOD) vigor, thiobarbituricacidα-method (TBA) method measures the content of malonaldehyde (MDA) content, enzyme-linked immunosorbent assay norepinephrine (NE), dopamine (DA) and 5-hydroxy tryptamine (5-HT), and chemical colorimetry measures choline acetyltransterase (ChAT) and acetylcholinesterase (AChE) vigor.
2.6 statistical procedures
Use SPSS18.0 software kit to carry out Data Management Analysis, during Morris water maze measures, orientation navigation test adopts the variance analysis of repeated measurement design, and other index adopts one factor analysis of variance.All experimental datas all use mean ± standard deviation (
) represent, compare between two between group with LSD-t inspection, there were significant differences for P < 0.05.
3 results
The comparison of 3.1 each group learning and memory in rats abilities
Compared with model group, except the 1st beyond the highest heavens, each dosage group of medicine and piracetam matched group significantly can shorten EL (P < 0.01); The distance that medicine middle and high dosage group and piracetam control rats are swum in target quadrant accounts for the percentage ratio of total distance and explores number of times comparatively model group showed increased (P < 0.05, P < 0.01); The curative effect of the middle and high dose drug group of the present invention is slightly better than piracetam matched group (table 1, table 2).Fig. 1 the 5th day typical original swimming track that be six groups of rats in orientation navigation test (wherein, figure a represents Normal group, figure b represents model group, figure c represents low dose group, dosage group during figure d represents, figure e represents high dose group, figure f represents piracetam matched group, I, II, III and IV represent first quartile, the second quadrant, third quadrant and fourth quadrant respectively, fourth quadrant black box represents the initiation site of rat motor, second quadrant black box represents the termination site of rat motor, and in the second quadrant, platform is hidden in circle representative; The figure a-curve of scheming in f represents the movement locus of rat).
Table 1 each group rat orientation navigation experiment average escape latency (s,
)
ap < 0.01vs matched group,
cp < 0.01vs model group
Table 2 respectively distance percentage ratio shared by group rat and explore number of times (
)
ap < 0.01vs matched group,
bp < 0.05,
cp < 0.01vs model group
3.2 medicines are on the impact of Senile Dementia Model Rats Hippocampus NE, DA, 5-HT
As shown in Table 3, compared with Normal group, in model group rats Hippocampus, NE, DA, 5-HT content obviously reduces (P < 0.01).After Drug therapy of the present invention, in medicine middle and high dosage group rat hippocampus, NE, DA, 5-HT content obviously increases (P < 0.05, P < 0.01).The curative effect of the middle and high dosage group of the present invention is slightly better than piracetam matched group, and the curative effect of piracetam matched group and low dose group of the present invention is close.
Table 3 each group rat hippocampus NE, DA, 5-HT content (
)
ap < 0.01vs matched group,
bp < 0.05,
cp < 0.01vs model group
3.3 medicines are on the impact of Senile Dementia Model Rats Hippocampus SOD, MDA, AChE, ChAT
As shown in Table 4, compared with Normal group, in model group rats Hippocampus, the activity of SOD obviously reduces, the content showed increased of MDA, and the activity of ChAT obviously declines, and AChE is active obviously increases (P < 0.01); The middle and high dosage group of medicine compares with model group with piracetam control rats, and in Hippocampus, the activity of SOD obviously increases, and the content of MDA obviously reduces, and ChAT is active obviously to be raised, and AChE is active obviously reduces (P < 0.01).The curative effect of the middle and high dosage group of the present invention is slightly better than piracetam matched group.
Table 4 rat hippocampus SOD, MDA, AChE, ChAT testing result (
n=10)
ap < 0.01vs matched group,
bp < 0.05,
cp < 0.01vs model group
4 discuss
Alzheimer disease, also known as Alzheimer (Alzheimer ' s disease, AD), is a kind of central nervous system's degeneration disease.This disease is with hypomnesis, Progressive symmetric erythrokeratodermia cognitive dysfunction for feature, and therefore in the Diagnosis and Treat of AD is evaluated, learning and memory level is most important index.Along with the quickening of aged tendency of population paces, explore the pathogenesis of AD, find the study hotspot that new prevention, medicine have become vast scientific research and clinical workers.Because the cause of disease of AD is complicated, pathogenesis is many-sided, and the medicine of single therapy target spot is more difficult obtains satisfied therapeutic effect.Chinese medicine, mostly containing plurality of active ingredients, has more wide prospect in the new drug excavating treatment AD.Theory of Chinese medical science is thought, the pathogenesis of AD is due to " deficiency of kidney essence, obstruction of collaterals by blood stasis ", the present composition is based on the kidney invigorating, do not stick to merely tonic, but pay attention to " benefits ", " tune " is double executes, and marrow is given birth to and brought up active, and can effective defeated cloth nourishing whole body, accomplish treatment must aim at the pathogenesis of disease, the Chinese traditional treatment meeting AD is theoretical.This experiment is by subcutaneous injection D-galactose associating bilateral hippocampus injection A β
25-35build dementia rats, Morris water maze result shows: compared with model group, except the 1st beyond the highest heavens, each dosage group of medicine and piracetam matched group significantly can shorten EL, and the distance that rat is swum in target quadrant accounts for the percentage ratio of total distance and explores number of times also showed increased.Illustrate that medicine of the present invention has improvement result to Senile Dementia Model Rats learning and memory function.
Current research shows, the generation cause of disease of AD mainly comprises oxidative stress hypothesis, the disorderly hypothesis of Monoamine Metabolism, the low hypothesis of choline function, apoptosis hypothesis, inflammation or immune hypothesis, amyloid beta deposition hypothesis etc.This experiment is attempted to inquire into from the following aspects the mechanism of action that medicine of the present invention improves Senile Dementia Model Rats learning and memory function.
Monoamine neurotransmitter directly or indirectly participates in the functional activity of brain, has important regulating action to learning and memory.NE is contacted by aixs cylinder and extensively projects cerebral central nervous system, regulates corticocerebral excitatory state, has extensive impact to awakening, emotion, cognitive function and motion.DA has various biological effect, as anti-lipid peroxidation, reduces oxidativestress damage; Increase brain blood flow supply; Improve the utilization etc. of brain neuroblastoma unit to glucose; The excitatory state of synergic adjustment body together with NE.5-HT, by regulating acetylcholine (ACh) release in cerebral tissue thus regulating the activity of cholinergic nerve of centrum, is the important neurotransmitter maintaining memory.Clinical study results shows, and the monoamine neurotransmitter in patients with Alzheimer disease cognitive function and body is in close relations, and in old human body, the minimizing of monoamine neurotransmitter and alzheimer disease is formed with direct relation.This experimental result is consistent with the result of this clinical research: in model group rats Hippocampus, NE, DA, 5-HT content compared with normal control rats obviously reduces.After Drug therapy of the present invention, in medicine middle and high dosage group rat hippocampus, NE, DA, 5-HT content obviously increases.And the curative effect of the middle and high dosage group of medicine of the present invention is better than piracetam matched group.
Recent research proves that oxidative stress plays an important role in the pathogenesis of AD, the generation of AD main unbalance with the oxidative and anti-oxidative ability of body self time body interior to produce too much free radical relevant.MDA is the mesostate of the lipid peroxide that oxygen-derived free radicals produces, and Chang Zuowei reflects the index that oxygen-derived free radicals generates.SOD is oxidant defense system important in body, at scavenging free radicals, plays a significant role to anti-oxidation stress aspect.Recent research result is pointed out, and early stage antioxidation measure occurs to have certain help to delaying senile dementia.This experimental result shows: model group rats cerebral tissue SOD is active obviously to be reduced, and MDA content obviously raises, and the middle and high dosage treatment group SOD activity of medicine obviously raises, and MDA content obviously reduces.It is active that this shows that medicine of the present invention can improve SOD in Senile Dementia Model Rats Hippocampus, reduces MDA content, in the removing of AD rat cerebral tissue oxygen-derived free radicals, have obvious effect, and treatment curative effect is better than piracetam.
Have important relation between cholinergic nerve of centrum system and learning and memory, cholinergic neuron disappearance causes the minimizing with the closely-related neurotransmitter ACh of learning and memory in brain, is one of important mechanisms of AD patient's learning memory disorder.Research display, in AD patient central nervous system, cholinergic nerve function obviously declines, and decline degree is closely related with the clinical symptoms order of severity of AD.ChAT is the key enzyme of synthesis ACh, and AChE is the key enzyme of degraded ACh.In the activity of ChAT and AChE and brain, the content of ACh is closely related, and both maintain the dynamic equilibrium of ACh content jointly.This experimental result finds: compared with Normal group, and model group rats Hippocampus ChAT is active obviously to decline, and AChE is active obviously to be increased; In the middle and high dosage group of medicine and piracetam control rats Hippocampus ChAT activity comparatively model group obviously raise, AChE is active obviously to be reduced.And the curative effect of the middle and high dosage group of the present invention is better than piracetam matched group.
In sum, medicine of the present invention is significantly improved Senile Dementia Model Rats learning memory disorder tool, and its mechanism with the activity increasing SOD, may reduce the content of MDA, improve monoamine neurotransmitter in Hippocampus relevant with the metabolism of cholinergic neurotransmitter.Its treatment curative effect is better than piracetam.
Claims (7)
1. treat the herbal composite of senile dementia for one kind, it is characterized in that, comprise following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae.
2. the herbal composite for the treatment of senile dementia according to claim 1, it is characterized in that, further, this herbal composite comprises following component according to mass parts: Rhizoma Acori Graminei 15 parts-20 parts, Radix Rehmanniae Preparata 15 parts-20 parts, Fructus Corni 15 parts-20 parts, Fructus Lycii 12 parts-18 parts, Radix Salviae Miltiorrhizae 10 parts-15 parts, Radix Polygalae 10 parts-15 parts, the Radix Astragali 12 parts-18 parts, Rhizoma Chuanxiong 10 parts-15 parts, Radix Angelicae Sinensis 12 parts-18 parts, 10 parts-15 parts, Poria, the Rhizoma Pinelliae 8 parts-12 parts, 8 parts-12 parts, Radix Glycyrrhizae.
3. the herbal composite for the treatment of senile dementia according to claim 1, it is characterized in that, further, this herbal composite comprises following component according to mass parts: Rhizoma Acori Graminei 18 parts, Radix Rehmanniae Preparata 18 parts, Fructus Corni 18 parts, Fructus Lycii 15 parts, Radix Salviae Miltiorrhizae 12 parts, Radix Polygalae 12 parts, the Radix Astragali 15 parts, Rhizoma Chuanxiong 12 parts, Radix Angelicae Sinensis 15 parts, 12 parts, Poria, the Rhizoma Pinelliae 10 parts, 10 parts, Radix Glycyrrhizae.
4. the herbal composite of the treatment senile dementia according to claim arbitrary in claim 1-3, is characterized in that, this herbal composite is prepared into capsule, pill, tablet, granule or water decoction.
5. treat a preparation method for the herbal composite of senile dementia, it is characterized in that, specifically implement according to following steps:
Step 1, weighing, weigh following component according to mass parts: Rhizoma Acori Graminei 8 parts-28 parts, Radix Rehmanniae Preparata 8 parts-28 parts, Fructus Corni 8 parts-28 parts, Fructus Lycii 5 parts-25 parts, Radix Salviae Miltiorrhizae 2 parts-22 parts, Radix Polygalae 2 parts-22 parts, the Radix Astragali 5 parts-25 parts, Rhizoma Chuanxiong 2 parts-22 parts, Radix Angelicae Sinensis 5 parts-25 parts, 2 parts-22 parts, Poria, the Rhizoma Pinelliae 1 part-20 parts, 1 part-20 parts, Radix Glycyrrhizae;
Step 2, prepare volatile oil, load weighted Rhizoma Acori Graminei, Radix Angelicae Sinensis, Ligusticum chuanxiong Hort are added respectively 6-10 times of water gaging and keep boiling 6-8h; Steam distillation obtains volatile oil, leaves and takes volatile oil, medicinal residues and medical material aqueous solution for subsequent use;
Step 3, by the respectively 6-10 times of soak by water 2 times of the medicinal residues in load weighted Radix Rehmanniae Preparata 8, Fructus Corni, Fructus Lycii, Radix Salviae Miltiorrhizae, Radix Polygalae, the Radix Astragali, Poria, the Rhizoma Pinelliae, Radix Glycyrrhizae and step 2, time is respectively 2-3h, and 2 decocting liquid and Rhizoma Acori Graminei, Radix Angelicae Sinensis, Rhizoma Chuanxiong aqueous solution are merged and concentrated;
Step 4, concentrated solution added ethanol leaves standstill, cold preservation 12-36 hour, it is 1.15-1.35 that decompression recycling ethanol concentrates density, makes extractum; Extractum and Rhizoma Acori Graminei, Radix Angelicae Sinensis volatile oil are added adjuvant, prepares the herbal composite for the treatment of senile dementia.
6. the preparation method of the herbal composite for the treatment of senile dementia according to claim 5, is characterized in that, the concentration of described ethanol is 50%-95%.
7. the preparation method of the herbal composite for the treatment of senile dementia according to claim 5, is characterized in that, this preparation method described prepares herbal composite and is prepared into capsule, pill, tablet, granule or water decoction.
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| CN105362615A (en) * | 2015-11-13 | 2016-03-02 | 蓝本祥 | Therapeutic drug for Guillain-Barre syndrome |
| CN105582394A (en) * | 2016-03-22 | 2016-05-18 | 广州三得医疗科技有限公司 | Chinese herb preparation containing folium artemisiae argyi and treating senile dementia and preparation method thereof |
| CN107184902A (en) * | 2017-05-21 | 2017-09-22 | 南京同仁堂药业有限责任公司 | A kind of Chinese medicine composition for being used to treat senile dementia |
| CN107596279A (en) * | 2017-11-07 | 2018-01-19 | 马军捷 | A kind of medicine for treating cerebral apoplexy sequela and senile dementia and preparation method thereof |
| CN108096422A (en) * | 2018-01-31 | 2018-06-01 | 横琴恩健生物科技有限公司 | A kind of Chinese medicine composition for treating senile dementia and preparation method thereof |
| CN111840431A (en) * | 2020-08-28 | 2020-10-30 | 孙学能 | Medicament for treating senile dementia |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105362615A (en) * | 2015-11-13 | 2016-03-02 | 蓝本祥 | Therapeutic drug for Guillain-Barre syndrome |
| CN105582394A (en) * | 2016-03-22 | 2016-05-18 | 广州三得医疗科技有限公司 | Chinese herb preparation containing folium artemisiae argyi and treating senile dementia and preparation method thereof |
| CN107184902A (en) * | 2017-05-21 | 2017-09-22 | 南京同仁堂药业有限责任公司 | A kind of Chinese medicine composition for being used to treat senile dementia |
| CN107596279A (en) * | 2017-11-07 | 2018-01-19 | 马军捷 | A kind of medicine for treating cerebral apoplexy sequela and senile dementia and preparation method thereof |
| CN108096422A (en) * | 2018-01-31 | 2018-06-01 | 横琴恩健生物科技有限公司 | A kind of Chinese medicine composition for treating senile dementia and preparation method thereof |
| CN111840431A (en) * | 2020-08-28 | 2020-10-30 | 孙学能 | Medicament for treating senile dementia |
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