CN104606254A - Method for processing periplaneta americana - Google Patents

Abstract

The invention provides a method for processing periplaneta americana. The method is characterized in that live periplaneta americana is put into boiling milk which is 1-10 times of that of the live periplaneta americana by weight, and dried. The periplaneta americana processed by the method is good in curative effect, small in peculiar smell during oral administration, good in mouthfeel and high in compliance of patients.

Description

The concocting method of a kind of periplaneta americana

Invention field

The present invention relates to medicine, particularly the concocting method of a kind of periplaneta americana, belong to medical art.

Background technology

Periplaneta americana belongs to Insecta, Blattaria Blattidae animal, and " Blatta seu periplaneta " is loaded in Shennong's Herbal for its popular name, the beginning of being used as medicine, and is listed in middle product, meaning " taste: salty, cold; Control: the hard cold and heat of Blood stasis, removing mass are poly-, laryngopharynx numbness, endogenous cold loss of fecundity ".Now there are some researches show, the large sickle in America has and has good tissue repair activity to various traumatic surface and digestive tract ulcer; There is boosting, improve sanguimotor activity; There is the activity of anti-bacteria and anti-virus; There is antitumor, strengthen immunologic function activity.

The concocting method of the large sickle in prior art America, is to kill by soak with ethanol after catching or boiling water scalds dead, dries or 60 DEG C of oven dry.With the large sickle in America that prior art is concocted, abnormal flavour is extra heavy, and mouthfeel is very poor, directly affects the compliance of patient.Improve periplaneta americana mouthfeel by concocting, it is very necessary for improving curative effect.

Summary of the invention

The object of this invention is to provide the concocting method of a kind of new periplaneta americana.

Technical scheme of the present invention is as follows:

A concocting method for periplaneta americana, is characterized in that: the periplaneta americana that lives puts into the milk that boils, dry.

The concocting method of a kind of periplaneta americana according to claim 1, is characterized in that: the consumption of milk is more than 1 times of periplaneta americana by weight.

So far the present invention is completed.The periplaneta americana good effect that the present invention obtains, oral abnormal flavour is little, and mouthfeel is good, and the compliance of patient is high.

Below by test example, usefulness of the present invention is described.Test example is intended to further illustrate beneficial effect of the present invention below, but not restriction of the present invention.

Test example one, different concocting method mouthfeel compare

1, by same mass products periplaneta americana, concoct by the following method.

1) soak with ethanol kills oven dry: kill by soak with ethanol, less than 60 DEG C oven dry, pulverizes;

2) milk that boils kills oven dry: the milk that boils putting into 2 times by weight, together dries for less than 60 DEG C, pulverizes.

2, mouthfeel evaluation is in the mouthfeel of medicine is evaluated, and sensory evaluation plays mastery reaction, and the sensation of people affects greatly by subjective factors, and language performance feels to have ambiguity, is difficult to use data representation accurately.So, this experiment is made up of 10 people appraises group through discussion, everyone soak with ethanol that warm water for oral use is mixed well respectively kills oven dry and the milk that boils kills the periplaneta americana of oven dry, evaluates the mouthfeel misty idea such as " very well ", " better ", " can accept ", " poor ", " very poor ".Evaluation result is in table 1

The periplaneta americana mouthfeel of the different concocting method of table 1 compares

	Very well	Better	Can accept	Poor	Very poor
						The milk that boils kills oven dry	2 people	3 people	5 people
Soak with ethanol kills oven dry				2 people	8 people

Result shows, and the milk that boils kills the periplaneta americana of oven dry, and oral abnormal flavour is little, and mouthfeel is good.

Test example two, different concocting method compare the protective effect that ethanol causes gastric mucosa damage.

1, by same mass products periplaneta americana, concoct by the following method.

1) kill lyophilizing by soak with ethanol, pulverize;

2) milk that boils kills lyophilizing: the milk that boils putting into 1 times by weight, together lyophilizing, pulverizes.

2, to ethanol cause gastric mucosa damage protective effect compare

Choose healthy SD rat, male and female half and half, weight 180-220g, be divided at random with soak with ethanol kill lyophilizing group, the milk that boils kills lyophilizing group, matched group, often organizes 10, every day gastric infusion 1 time, 1 2mg/kg(solid contents after testing milk lyophilizing is 12%, the milk that boils kills the periplaneta americana of together lyophilizing in sterling), continuous 7 days, matched group gave equivalent distilled water.Before experiment, the first fasting 48h of rat, freely drinks water, after last administration, 1h every rat oral gavage dehydrated alcohol 1mL/ only, take off cervical vertebra after 1h and put to death animal, close cardia by clip, inject 1% formaldehyde 5mL from pylorus, folder closes pylorus again, put into 1% formalin and fix 10min, cut off coat of the stomach along greater gastric curvature, measure damaged length, as gastric mucosal damage index, and suppression ratio is calculated using damaged length (width is greater than 1 mm to be doubled) summation.The results are shown in Table 2.

Suppression ratio (%)=(matched group ulcer index one administration group ulcer index) ÷ matched group ulcer index × 100%.

The different concocting method of table 2 compares the protective effect that ethanol causes gastric mucosa damage

Group	Dosage	Gastric mucosal damage index (mm)	Suppression ratio (%)
				Matched group		123.4±15.2
Soak with ethanol kills lyophilizing group	2mg/kg	54.2±13.6	56.08
				The milk that boils kills lyophilizing group	2mg/kg	46.8±10.8	60.45

Result shows, the periplaneta americana that the milk that boils kills lyophilizing is better than the periplaneta americana of lyophilizing to the protective effect that ethanol causes gastric mucosa damage.

Test example three, different concocting method liver protective effect compare

1, by same mass products periplaneta americana, concoct by the following method.

1) dry: scald with boiling water dead, less than 60 DEG C oven dry, pulverize;

2) milk that boils kills oven dry: the milk that boils putting into 5 times by weight, less than 60 DEG C oven dry, pulverizes.

2, to CCl ₄the impact test of caused Liver Damage in Rats.

The healthy SD rat of body weight 180-220g, is divided into oven dry group at random, the milk that boils kills oven dry group, matched group, often organizes 10; Contrast with test group in experiment the 1st, 5 days subcutaneous injection 50%CCl ₄vegetable oil suspension 5mL/kg, from experiment to start day respectively every day gastric infusion 1 time, 1 3g/kg(milk that boils kills the periplaneta americana of oven dry in sterling), matched group gives equal-volume distilled water, continuous 9 days, drug withdrawal 24 hours eye sockets got blood, survey serum glutamic pyruvic transminase with reitman-frankel method.The results are shown in Table 3.

The different concocting method of table 3 is to CCl ₄the impact of caused hepatic injury rat blood serum glutamate pyruvate transaminase

Group	Dosage (g/kg)	Glutamate pyruvate transaminase in serum
			Matched group		352.7±123.6
Oven dry group	3	185.2±109.4
			The milk that boils kills oven dry group	3	168.4±100.5

Result shows, the milk that boils kills the periplaneta americana of oven dry to rat CCl ₄the protective effect of caused hepatic injury is better than the periplaneta americana of oven dry.

The periplaneta americana anticancer effect of test example four, different concocting method compares

1, with a collection of periplaneta americana, will concoct by the following method.

1) lyophilizing: kill by soak with ethanol, lyophilizing, pulverizes;

2) milk that boils kills lyophilizing: the milk that boils putting into 3 times by weight kills, together lyophilizing, pulverizes.

2, the periplaneta americana of different concocting method anti-human lung source adenocarcinoma cell SPCA-1 effectiveness comparison.

1) prepare the periplaneta americana powder that American-cockroach-extract gets different concocting method respectively, respectively decoct with water twice, add water 8 times amount for the first time, decocts 2 hours, add water 8 times amount for the second time, decocts 1 hour, merges medicinal liquid, medicinal liquid filters, and get filtrate, being concentrated into relative density is 1.10(60 DEG C) time, adding ethanol makes alcohol content reach 75%, precipitation, leaves standstill 36 hours, Aspirate supernatant, reclaims ethanol, concentrated, dry, pulverize, obtain the American-cockroach-extract of different concocting method.

2) tumor cell line people lung source adenocarcinoma cell SPCA-1, uses RPMI-1649 culture fluid, 37 DEG C, 5%CO2, and relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.

3) method MTT colorimetric method for determining extract is to the inhibitory action of growth of tumour cell.To take the logarithm trophophase cell, be mixed with cell suspension with fresh RPMI-1640 culture fluid, suspension cell 1 × 105/ml attached cell 0.8 × 105/ml.Suspension cell is inoculated in after adding variable concentrations Experimental agents respectively in 96 well culture plates; Attached cell is first inoculated in 96 well culture plates, adds variable concentrations Experimental agents respectively after every hole 90 μ l24h.Final volume is every hole 100 μ l and often group establishes 3 parallel holes, grouping: by reagent group (culture fluid+cell suspension+variable concentrations is by reagent), negative control group (culture fluid+cell suspension), medicine color comparator group (culture fluid+variable concentrations is by reagent), blank group (culture fluid+normal saline).Tested concentration is followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 DEG C, after cultivating 72h in 5%CO2 incubator, after adding MTT solution 10 μ l concussion mixing to every hole, continue to cultivate 4h, add SDS90 μ l to stop cultivating, 37 DEG C are spent the night, and then on micro oscillator, shake 10min under room temperature, microplate reader measures the absorbance (OD value) at 570nm wavelength place, experiment repetition 3 times.

Calculate growth inhibition ratio as follows:

Growth inhibition ratio (%)=(N group OD average-B organizes OD average)-(T group OD average-TC organizes OD average)/N group OD average-B organizes OD average × 100%.

4) suppression ratio the results are shown in Table 4.

The different concocting method of table 4 is to the suppression ratio results contrast of SPCA-1

Suppression ratio (%)	10（μg/ml）	30（μg/ml）	50（μg/ml）
				Lyophilizing group	64.27	80.53	89.48
The milk that boils kills lyophilizing group	71.16	89.47	93.25

Result shows, the periplaneta americana that the milk that boils kills lyophilizing is better than the periplaneta americana of lyophilizing to people's lung source adenocarcinoma cell SPCA-1 inhibitory action.

Detailed description of the invention

embodiment 1

Live periplaneta americana 2.5kg, and the milk that boils putting into 2.5kg kills, together lyophilizing.

embodiment 2

Live periplaneta americana 2kg, and the milk that boils putting into 4kg kills, and together dries for less than 60 DEG C.

embodiment 3

Live periplaneta americana 5kg, puts into 15kg and to boil milk, together put in freeze dryer, be refrigerated to less than-10 DEG C, be evacuated to below drying chamber pressure 30Pa, subliming by heating; The temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.

embodiment 4

Live periplaneta americana 10kg, puts into 100kg and to boil milk, less than 60 DEG C, together vacuum drying.

embodiment 5

Live periplaneta americana 1kg, puts into 8kg and to boil milk, less than 60 DEG C, together vacuum drying.

embodiment 6

Live periplaneta americana 3kg, puts into 8kg and to boil milk, together lyophilizing.

embodiment 7

Live periplaneta americana 3kg, puts into 15kg and to boil milk, together microwave drying.

Claims (2)

1. a concocting method for periplaneta americana, is characterized in that: the periplaneta americana that lives puts into the milk that boils, dry.

2. the concocting method of a kind of periplaneta americana according to claim 1, is characterized in that: the consumption of milk is more than 1 times of periplaneta americana by weight.