CN104367595A - Application of American cockroach dried by different methods in preparation of medicines - Google Patents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2009—Inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
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Abstract
The invention relates to application of American cockroach dried by different methods in preparation of medicines. The American cockroach is used for preparing medicines for treating peptic ulcer. The caught American cockroach is killed by using ethanol, and the freeze-drying effect is the best. The American cockroach is used for preparing medicines for treating and resisting cancers, the caught American cockroach is soaked in ethanol to be killed or scalded to die in boiling water, and the freeze-drying effect is the best. The American cockroach is used for preparing medicines for treating cardiovascular and cerebrovascular diseases, the caught American cockroach is soaked in ethanol to be killed or scalded to die in boiling water, and the drying effect at the temperature of 60-90 DEG C is the best.
Description
Invention field
The present invention relates to medicine method for making, particularly the dry periplaneta americana of distinct methods is preparing the application in medicine, belongs to medical art.
Background technology
The large sickle in America (Periplaneta american) belongs to Insecta, and Fei Lian section, is commonly called as Blatta seu periplaneta.The large sickle in America sees Shennong's Herbal at first as medicinal, and for controlling, bloody sputum disease, cold and heat, removing mass are poly-, laryngopharynx numbness, endogenous cold loss of fecundity.At present with the large sickle in America for medicinal raw material, have the Chinese patent medicines such as rehabilitation is new, Xinmailong, liver dragon in market sale.
Traditional Chinese medicine drying has very long history in China, the Chinese herbal medicine of drying can keep certain medicinal ingredient and not easily putrid and deteriorated, the dry important measures as ensureing Chinese herbal medicine quality, it is a requisite technical process in Chinese herbal medicine processing, it is in close relations that dry and Chinese medicine is produced, and dry method will directly affect the curative effect of product.The drying means of the large sickle in existing document America, kills by soak with ethanol after seizure or boiling water scalds dead, dries or 60 DEG C of oven dry.
Summary of the invention
The object of this invention is to provide the dry periplaneta americana of distinct methods and preparing the application in medicine, the dry periplaneta americana of distinct methods is respectively used to the medicine preparing treatment digestive tract ulcer, cancer, cardiovascular and cerebrovascular vessel.
The present invention is achieved in that
The dry periplaneta americana of distinct methods is preparing the application in medicine, it is characterized in that: the dry periplaneta americana of distinct methods is respectively used to the medicine preparing treatment digestive tract ulcer, cancer, cardiovascular and cerebrovascular vessel.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment digestive tract ulcer medicine is: kill by soak with ethanol after seizure, lyophilizing.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cancer therapy drug is: kill by soak with ethanol after seizure or boiling water scalds and kills, lyophilizing.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cardiovascular and cerebrovascular diseases medicament is: kill by soak with ethanol after seizure or boiling water scalds and kills, 60 DEG C-90 DEG C oven dry.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: lyophilizing is the periplaneta americana killed, puts in freeze dryer, be refrigerated to less than-10 DEG C, be evacuated to below drying chamber pressure 30Pa, subliming by heating; The temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cardiovascular and cerebrovascular diseases medicament is: kill by soak with ethanol after seizure or boiling water scalds and kills, 80 DEG C of oven dry.
So far complete the present invention, with the medicine of periplaneta americana preparation treatment digestive tract ulcer, kill by soak with ethanol after seizure, effect is best.With the anticancer medicine of periplaneta americana preparation treatment, to kill or boiling water scalds and kills after seizure by soak with ethanol, effect is best.To kill by soak with ethanol after catching with the medicine of periplaneta americana preparation treatment cardiovascular and cerebrovascular disease or boiling water scalds and kills, 60 DEG C of-90 DEG C of drying effects are best.
Further illustrate usefulness of the present invention below by test example, test example is intended to further illustrate usefulness of the present invention, but not limitation of the present invention.
One, the periplaneta americana of distinct methods lyophilizing causes the protective effect test of gastric mucosa damage to ethanol.
1, sample is prepared
By the same a collection of periplaneta americana caught, process by the following method.
1) ethanol kills lyophilizing: kill by soak with ethanol, puts in freeze dryer, is refrigerated to less than-20 DEG C, is evacuated to below drying chamber pressure 45Pa, subliming by heating, and the temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
2) boiling water scalds dead lyophilizing: scald dead with boiling water, put in freeze dryer, be refrigerated to less than-20 DEG C, be evacuated to below drying chamber pressure 45Pa, subliming by heating, the temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
3) ethanol kills oven dry: kill by soak with ethanol, 80 DEG C of oven dry.
4) boiling water scalds and kills oven dry: scald with boiling water and kill, 80 DEG C of oven dry.
2, the periplaneta americana of distinct methods drying is compared the protective effect that ethanol causes gastric mucosa damage.
Choose healthy SD rat, male and female half and half, weight 180-220g, be divided into matched group at random, ethanol kills lyophilizing group, boiling water scalds dead lyophilizing group, often organize 10, every day gastric infusion 1 time, 1 2mg/kg, continuous 7 days, matched group gave equivalent distilled water.Before experiment, first fasting 48 h of rat, freely drinks water, after last administration, 1h every rat oral gavage dehydrated alcohol 1mL/ only, take off cervical vertebra after 1h and put to death animal, close cardia by clip, inject 1% formaldehyde 5mL from pylorus, folder closes pylorus again, put into 1% formalin and fix 10min, cut off coat of the stomach along greater gastric curvature, measure damaged length, as gastric mucosal damage index, and suppression ratio is calculated using damaged length (width is greater than 1 mm to be doubled) summation.The results are shown in Table 1.
Suppression ratio (%)=(matched group ulcer index one administration group ulcer index) ÷ matched group ulcer index × 100%.
The periplaneta americana of table 1 distinct methods drying is compared the protective effect that ethanol causes gastric mucosa damage
| Group | Dosage | Gastric mucosal damage index (mm) | Suppression ratio (%) |
| Matched group | 99.4±25.4 | ||
| Ethanol kills lyophilizing | 2mg/kg | 59.4±24.5 | 40.2 |
| Boiling water scalds dead lyophilizing | 2mg/kg | 84.8±20.4 | 14.7 |
| Ethanol kills oven dry | 2mg/kg | 87.5±20.4 | 12.0 |
| Boiling water scalds and kills oven dry | 2mg/kg | 88.1±20.4 | 11.4 |
Result shows, the periplaneta americana of distinct methods drying causes gastric mucosa damage to ethanol all has protective effect, and it is best that ethanol kills the effect of lyophilizing periplaneta americana.
Two, the periplaneta americana of distinct methods drying anti-human lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 test.
1, sample is prepared
One, the same a collection of periplaneta americana will caught, processes by the following method.
1) ethanol kills oven dry: kill by soak with ethanol, dries following oven dry for 60 DEG C;
2) ethanol kills lyophilizing: kill by soak with ethanol, puts in freeze dryer, is refrigerated to less than-20 DEG C, is evacuated to below drying chamber pressure 45Pa, subliming by heating, the temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry, take out, pack;
3) boiling water scalds dead lyophilizing: boiling water scalds dead, puts in freeze dryer, is refrigerated to less than-20 DEG C, be evacuated to below drying chamber pressure 45Pa, subliming by heating, the temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry, take out, pack;
4) boiling water scalds dead: boiling water scalds dead, moist.
2, the periplaneta americana of distinct methods drying anti-human lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562 test.
1, prepare American-cockroach-extract to get periplaneta americana prepared by different said method respectively and pulverize, respectively decoct with water twice, 8 times amount that add water for the first time (boiling water scalds dead periplaneta americana in dry product, lower same), decoct 2 hours, add water 8 times amount for the second time, decocts 1 hour, merges medicinal liquid, medicinal liquid filters, get filtrate, being concentrated into relative density is 1.10(60 DEG C) time, add ethanol and make alcohol content reach 75%, precipitation, leave standstill 36 hours, Aspirate supernatant, reclaim ethanol, concentrated, dry, pulverize, obtain the American-cockroach-extract of distinct methods drying.
2, tumor cell line people lung source adenocarcinoma cell SPCA-1, human hepatoma cell line HepG2 and people's chronic myelogenous leukemia cell K562, use RPMI-1649 culture fluid, 37 DEG C, 5%CO2, and relative humidity 100% is cultivated, and attached cell 0.25% trypsinization goes down to posterity.
3, method MTT colorimetric method for determining extract is to the inhibitory action of growth of tumour cell.To take the logarithm trophophase cell, be mixed with cell suspension with fresh RPMI-1640 culture fluid, suspension cell 1 × 105/ml attached cell 0.8 × 105/ml.Suspension cell is inoculated in after adding variable concentrations Experimental agents respectively in 96 well culture plates; Attached cell is first inoculated in 96 well culture plates, adds variable concentrations Experimental agents respectively after every hole 90 μ l24h.Final volume is every hole 100 μ l and often group establishes 3 parallel holes, grouping: by reagent group (culture fluid+cell suspension+variable concentrations is by reagent), negative control group (culture fluid+cell suspension), medicine color comparator group (culture fluid+variable concentrations is by reagent), blank group (culture fluid+normal saline).Tested concentration is followed successively by 10 μ g/ml, 30 μ g/ml, 50 μ g/ml.Put 37 DEG C, after cultivating 72h in 5%CO2 incubator, after adding MTT solution 10 μ l concussion mixing to every hole, continue to cultivate 4h, add SDS90 μ l to stop cultivating, 37 DEG C are spent the night, and then on micro oscillator, shake 10min under room temperature, microplate reader measures the absorbance (OD value) at 570nm wavelength place, experiment repetition 3 times.
Calculate growth inhibition ratio as follows:
Growth inhibition ratio (%)=(N group OD average-B organizes OD average)-(T group OD average-TC organizes OD average)/N group OD average-B organizes OD average × 100%.
4, suppression ratio the results are shown in Table 2, table 3, table 4.
The suppression ratio result of table 2 couple SPCA-1
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Ethanol kills oven dry | 42.76 | 57.52 | 64.47 |
| Ethanol kills lyophilizing | 59.42 | 75.28 | 84.63 |
| Boiling water scalds dead lyophilizing | 59.43 | 75.29 | 84.62 |
| Boiling water scalds dead | 42.74 | 57.53 | 64.45 |
The suppression ratio result of table 3 couple HepG2
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Ethanol kills oven dry | 44.74 | 56.31 | 62.38 |
| Ethanol kills lyophilizing | 62.45 | 69.26 | 87.54 |
| Boiling water scalds dead lyophilizing | 62.46 | 69.25 | 87.53 |
| Boiling water scalds dead | 44.71 | 56.32 | 62.39 |
The suppression ratio result of table 4 couple K562
| Suppression ratio (%) | 10（μg/ml） | 30（μg/ml） | 50（μg/ml） |
| Ethanol kills oven dry | 51.62 | 64.53 | 76.49 |
| Ethanol kills lyophilizing | 66.85 | 79.64 | 87.38 |
| Boiling water scalds dead lyophilizing | 66.86 | 79.62 | 87.36 |
| Boiling water scalds dead | 51.60 | 64.54 | 76.51 |
Result shows, ethanol kills lyophilizing and boiling water and scalds dead lyophilizing periplaneta americana and all kill oven dry and boiling water compared with ethanol to the propagation of SPCA-1, HepG2, K562 cell and scald dead periplaneta americana and have stronger inhibitory action.
Three, the active constituent content test of the periplaneta americana Cardiovarscular of different temperatures drying
1, processed sample
By the same a collection of periplaneta americana caught, process by the following method.
1) 50 DEG C of oven dry: kill by soak with ethanol, 50 DEG C of oven dry;
2) 55 DEG C of oven dry: kill by soak with ethanol, 55 DEG C of oven dry;
3) 60 DEG C of oven dry: kill by soak with ethanol, 60 DEG C of oven dry;
4) 65 DEG C of oven dry: kill by soak with ethanol, 65 DEG C of oven dry;
5) 70 DEG C of oven dry: kill by soak with ethanol, 70 DEG C of oven dry;
6) 75 DEG C of oven dry: kill by soak with ethanol, 75 DEG C of oven dry;
7) 80 DEG C of oven dry: kill by soak with ethanol, 80 DEG C of oven dry;
8) 85 DEG C of oven dry: kill by soak with ethanol, 85 DEG C of oven dry;
9) 90 DEG C of oven dry: kill by soak with ethanol, 90 DEG C of oven dry;
10) 95 DEG C of oven dry: kill by soak with ethanol, 95 DEG C of oven dry;
2, the periplaneta americana mannitol content of different temperatures drying measures
Sample thief is about 1g, accurately weighed, put in 150ml round-bottomed flask, precision adds ethanol 100ml, weighed weight, reflux 2 hours, let cool, the weight of less loss is supplied with ethanol, filter, precision measures subsequent filtrate 5ml, put in iodine flask, precision adds sodium metaperiodate (potassium) solution [get sulfuric acid solution (1 → 20) 90ml and sodium metaperiodate (potassium) solution (2.3 → 1000) 110ml is mixed] 50 ml, put heating in water bath 15 minutes, add potassium iodide test solution 10ml, close plug, place 5 minutes, liquid (0.05mol/L) titration is determined with sodium thiosulfate, during to nearly terminal, add starch indicator solution 1ml, continue to be titrated to blue disappearance, and the result blank assay of titration is corrected.Every 1ml sodium thiosulfate volumetric solution (0.05mol/L) is equivalent to the mannitol of 0.91mg.The results are shown in Table 5.
The periplaneta americana mannitol content measurement result of table 5 different temperatures drying
| Temperature | 50℃ | 55℃ | 60℃ | 65℃ | 70℃ |
| Content (mg/g) | 7.36 | 7.38 | 9.75 | 10.42 | 11.63 |
| Temperature | 75℃ | 80℃ | 85℃ | 90℃ | 95℃ |
| Content (mg/g) | 12.54 | 13.86 | 12.31 | 10.25 | 6.83 |
Result shows, periplaneta americana is dried lower than 60 DEG C and is greater than 90 DEG C of oven dry, and mannitol content is obviously low, and 80 DEG C of oven dry mannitol contents are the highest.
3, the periplaneta americana Determination of Adenosine of different temperatures drying
Adenosine measures according to Chinese Pharmacopoeia annex VI D high-efficient phase chromatogram method.
Chromatographic condition and system suitability take octadecylsilane chemically bonded silica as filler, with acetonitrile-0.04mol/L potassium dihydrogen phosphate (5:95) for mobile phase; Determined wavelength is 260nm.Number of theoretical plate calculates should be not less than 3000 by adenosine peak.
The preparation of reference substance solution gets adenosine reference substance in right amount, accurately weighed, adds 0.5% phosphoric acid solution and makes the solution of every 1ml containing 12 μ g, to obtain final product.
The sample thief of preparing of need testing solution is about 0.5g, accurately weighed, and put in tool plug conical flask, add diethyl ether 20ml, close plug, soak 30 minutes, filter, close plug, discards ether, residue volatilizes, and together with in filter paper one juxtaposition tool plug conical flask, precision adds 0.5% phosphoric acid solution 50ml, close plug, weighed weight, supersound process (power 250W, frequency 33kHz) 30 minutes, lets cool, weighed weight again, supplies the weight of less loss, shakes up with 0.5% phosphoric acid solution, leave standstill, get supernatant, filter, get subsequent filtrate, to obtain final product.
Algoscopy is accurate respectively draws reference substance solution and each 10 μ l of need testing solution, injection liquid chromatography, measures.The results are shown in Table 6.
The periplaneta americana Determination of Adenosine result of table 6 different temperatures drying
| Temperature | 50℃ | 55℃ | 60℃ | 65℃ | 70℃ |
| Content (mg/g) | 0.08 | 0.08 | 0.13 | 0.25 | 0.31 |
| Temperature | 75℃ | 80℃ | 85℃ | 90℃ | 95℃ |
| Content (mg/g) | 0.45 | 0.52 | 0.50 | 0.34 | 0.11 |
Result shows, periplaneta americana is dried lower than 60 DEG C and is greater than 90 DEG C of oven dry, and adenosine content is obviously low, and 80 DEG C of oven dry adenosine contents are the highest.
4, ethanol kills or boiling water scalds periplaneta americana mannitol and the Determination of Adenosine of the synthermal drying of hipocratic face
1) processed sample
By the same a collection of periplaneta americana caught, process by the following method.
1. ethanol kills 80 DEG C of oven dry: kill by soak with ethanol, 80 DEG C of oven dry;
2. boiling water scalds dead 80 DEG C of oven dry: scald dead with boiling water, 80 DEG C of oven dry;
2) distinct methods kills periplaneta americana mannitol and the Determination of Adenosine of identical temperature drying
Measure mannitol and adenosine content with said method respectively, the results are shown in Table 7.
Table 7 distinct methods kills periplaneta americana mannitol and the Determination of Adenosine result of identical temperature drying
| Ethanol kills 80 DEG C of oven dry | Boiling water scalds dead 80 DEG C of oven dry | |
| Mannitol content (mg/g) | 13.84 | 13.83 |
| Adenosine content (mg/g) | 0.53 | 0.54 |
Result shows, distinct methods kill the periplaneta americana mannitol of identical temperature drying and Determination of Adenosine result basically identical.
As well known to those skilled in the art, adenosine is endogenous purine nucleoside, can make atrioventricular node delayed conduction, and block atrioventricular nodal reentry approach, paroxysmal supraventricular tachycardia patient recovers normal sinus rhythm.In periplaneta americana, nucleoside has and improves microcirculation, increases coronary artery blood flow and increase cardiac output, reduces the effect such as pressure pulmonary artery diastolic pressure and pulmonary capillary wedge according to the literature.Mannitol is being pharmaceutically good diuretic, reduces intracranial pressure, intraocular pressure and treatment kidney medicine, dehydrant, is usually used in the cerebral edema that treatment a variety of causes causes.
According to result of the test, and then known, with the medicine of periplaneta americana preparation treatment cardiovascular and cerebrovascular disease, to kill or boiling water scalds and kills after seizure by soak with ethanol, 60 DEG C of-90 DEG C of drying effects are best.
Detailed description of the invention
The dry periplaneta americana of distinct methods is preparing the application in medicine, it is characterized in that: the dry periplaneta americana of distinct methods is respectively used to the medicine preparing treatment digestive tract ulcer, cancer, cardiovascular and cerebrovascular vessel.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment digestive tract ulcer medicine is: kill by soak with ethanol after seizure, lyophilizing.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cancer therapy drug is: kill by soak with ethanol after seizure or boiling water scalds and kills, lyophilizing.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cardiovascular and cerebrovascular diseases medicament is: kill by soak with ethanol after seizure or boiling water scalds and kills, 60 DEG C-90 DEG C oven dry.
The dry periplaneta americana of distinct methods of the present invention is preparing the application in medicine, it is characterized in that: lyophilizing is the periplaneta americana killed, puts in freeze dryer, be refrigerated to less than-10 DEG C, be evacuated to below drying chamber pressure 30Pa, subliming by heating; The temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
embodiment 1
Periplaneta americana kills by soak with ethanol after catching, and put in freeze dryer, be refrigerated to-20 DEG C, be evacuated to drying chamber pressure 30Pa, subliming by heating, the temperature of distillation controls below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.Pulverize, make powder, be used for the treatment of gastric ulcer.
embodiment 2
Periplaneta americana kills by soak with ethanol after catching, and put in freeze dryer, be refrigerated to-28 DEG C, be evacuated to drying chamber pressure 50Pa, subliming by heating, the temperature of distillation controls at 50 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
Get lyophilizing periplaneta americana and be crushed to coarse powder, add 6 times of water gagings, stir extraction 4 hours, totally 2 times, merge extractive liquid, at 20 DEG C, filter, centrifugal, centrifugal liquid loads freeze drying box, lyophilizing.Pulverize, wet granulation, reduced vacuum is dry, granulate, and add Pulvis Talci in right amount, tabletting, is used for the treatment of ulcerative colitis.
embodiment 3
Periplaneta americana kills by soak with ethanol after catching, and put in freeze dryer, put in freeze dryer, be refrigerated to-32 DEG C, be evacuated to drying chamber pressure 50Pa, subliming by heating, the temperature of distillation controls at 50 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
Get lyophilizing periplaneta americana, with water extraction 2 times, first time adds 8 times of water gagings, extracts 2 hours, and second time adds 6 times amount water extraction 1 hour, merge extractive liquid, filters, concentrated, dry, pulverize, wet granulation, and drying, granulate, shape capsule, is used for the treatment of hepatocarcinoma.
embodiment 4
Scald dead after periplaneta americana catches with boiling water, put in freeze dryer, be refrigerated to-20 DEG C, be evacuated to drying chamber pressure 30Pa, subliming by heating, the temperature of distillation controls below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
Get lyophilizing periplaneta americana, decoct with water twice, add water 8 times amount for the first time, decocts 2 hours, add water 8 times amount for the second time, decocts 1 hour, merges medicinal liquid, and medicinal liquid filters, get filtrate, being concentrated into relative density is 1.10(60 DEG C) time, add ethanol and make alcohol content reach 75%, precipitation, leave standstill 36 hours, Aspirate supernatant, reclaim ethanol, concentrated, dry, pulverize, granulate, granulate, obtains granule, is used for the treatment of pulmonary carcinoma.
embodiment 5
The periplaneta americana caught, kills by soak with ethanol, 60 DEG C of oven dry.Make oral liquid, be used for the treatment of heart failure.
embodiment 6
The periplaneta americana caught, scalds dead, 80 DEG C of oven dry with boiling water.Decoct with water twice, add water 8 times amount for the first time, decocts 2 hours, and add water 8 times amount for the second time, decoct 1 hour, merge medicinal liquid, medicinal liquid filters, and gets filtrate, being concentrated into relative density is 1.10(60 DEG C) time, add ethanol and make alcohol content reach 75%, precipitation, leave standstill 36 hours, Aspirate supernatant, reclaims ethanol, concentrated, dry, pulverize, granulate, granulate, obtains granule, is used for the treatment of coronary heart disease.
embodiment 7
The periplaneta americana caught, scalds dead, 90 DEG C of oven dry with boiling water.Make tablet, be used for the treatment of cerebral thrombosis.
Claims (6)
1. the dry periplaneta americana of distinct methods is preparing the application in medicine, it is characterized in that: the dry periplaneta americana of distinct methods is respectively used to the medicine preparing treatment digestive tract ulcer, cancer, cardiovascular and cerebrovascular vessel.
2. the dry periplaneta americana of distinct methods according to claim 1 is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment digestive tract ulcer medicine is: kill by soak with ethanol after seizure, lyophilizing.
3. the dry periplaneta americana of distinct methods according to claim 1 is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cancer therapy drug is: kill by soak with ethanol after seizure or boiling water scalds and kills, lyophilizing.
4. the dry periplaneta americana of distinct methods according to claim 1 is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cardiovascular and cerebrovascular diseases medicament is: kill by soak with ethanol after seizure or boiling water scalds and kills, 60 DEG C-90 DEG C oven dry.
5. the dry periplaneta americana of distinct methods according to claim 1 is preparing the application in medicine, it is characterized in that: lyophilizing is the periplaneta americana killed, puts in freeze dryer, be refrigerated to less than-10 DEG C, be evacuated to below drying chamber pressure 30Pa, subliming by heating; The temperature of distillation should control below 40 DEG C, and the temperature of resolution phase controls below 45 DEG C, to dry.
6. the dry periplaneta americana of distinct methods according to claim 4 is preparing the application in medicine, it is characterized in that: the drying means of preparation treatment cardiovascular and cerebrovascular diseases medicament is: kill by soak with ethanol after seizure or boiling water scalds and kills, 80 DEG C of oven dry.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104872381A (en) * | 2015-06-05 | 2015-09-02 | 袁玥 | Special feed for young mantises artificially cultured |
| CN104872080A (en) * | 2015-06-05 | 2015-09-02 | 袁玥 | Artificial feeding method of mantises |
| CN104905053A (en) * | 2015-06-02 | 2015-09-16 | 安徽诺阳禽业有限公司 | Biological feed rich in cockroach powder and used for health duck and preparation method of biological feed |
| CN104957397A (en) * | 2015-06-05 | 2015-10-07 | 袁玥 | Special feed for artificial breeding adult mantis |
| CN104957391A (en) * | 2015-06-02 | 2015-10-07 | 安徽诺阳禽业有限公司 | Cockroach powder-rich egg-laying duck special feed |
| CN104957392A (en) * | 2015-06-02 | 2015-10-07 | 安徽诺阳禽业有限公司 | Egg-producing goose special feed rich in cockroach powder and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104905053A (en) * | 2015-06-02 | 2015-09-16 | 安徽诺阳禽业有限公司 | Biological feed rich in cockroach powder and used for health duck and preparation method of biological feed |
| CN104957391A (en) * | 2015-06-02 | 2015-10-07 | 安徽诺阳禽业有限公司 | Cockroach powder-rich egg-laying duck special feed |
| CN104957392A (en) * | 2015-06-02 | 2015-10-07 | 安徽诺阳禽业有限公司 | Egg-producing goose special feed rich in cockroach powder and preparation method thereof |
| CN104982652A (en) * | 2015-06-02 | 2015-10-21 | 安徽诺阳禽业有限公司 | Processing method for food cockroach |
| CN104872381A (en) * | 2015-06-05 | 2015-09-02 | 袁玥 | Special feed for young mantises artificially cultured |
| CN104872080A (en) * | 2015-06-05 | 2015-09-02 | 袁玥 | Artificial feeding method of mantises |
| CN104957397A (en) * | 2015-06-05 | 2015-10-07 | 袁玥 | Special feed for artificial breeding adult mantis |
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