CN104604772A - Method for observing, collecting and separating germ cells of pond-cultured coilia ectenes - Google Patents
Method for observing, collecting and separating germ cells of pond-cultured coilia ectenes Download PDFInfo
- Publication number
- CN104604772A CN104604772A CN201510056409.XA CN201510056409A CN104604772A CN 104604772 A CN104604772 A CN 104604772A CN 201510056409 A CN201510056409 A CN 201510056409A CN 104604772 A CN104604772 A CN 104604772A
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- CN
- China
- Prior art keywords
- fertilized egg
- tailed anchovy
- cutter long
- pond
- coilia ectenes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 241001460967 Coilia nasus Species 0.000 title abstract description 12
- 210000004602 germ cell Anatomy 0.000 title abstract 6
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- 241001430403 Coilia grayii Species 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 102000002322 Egg Proteins Human genes 0.000 claims description 11
- 108010000912 Egg Proteins Proteins 0.000 claims description 11
- 210000004681 ovum Anatomy 0.000 claims description 11
- 230000004313 glare Effects 0.000 claims description 5
- ZXJXZNDDNMQXFV-UHFFFAOYSA-M crystal violet Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1[C+](C=1C=CC(=CC=1)N(C)C)C1=CC=C(N(C)C)C=C1 ZXJXZNDDNMQXFV-UHFFFAOYSA-M 0.000 claims description 4
- 229960001235 gentian violet Drugs 0.000 claims description 4
- 230000012447 hatching Effects 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000017448 oviposition Effects 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 abstract 2
- 235000013601 eggs Nutrition 0.000 description 21
- 238000011160 research Methods 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 235000019688 fish Nutrition 0.000 description 4
- 239000013505 freshwater Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 241001125843 Trichiuridae Species 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for observing, collecting and separating germ cells of pond-cultured coilia ectenes. The method comprises the steps of selecting a coilia ectenes spawning pond, preparing a simple coilia ectenes germ cell observation device and an electric cell collecting device, processing, separating and cleaning the germ cells and the like. By means of the method, the egg-laying time of the coilia ectenes can be accurately judged, the problem that the germ cells of coilia ectenes are small and transparent, so that the germ cells are difficult to observe directly with naked eyes and must be observed through a microscope is solved, and a simple and practical core technology for breed of the coilia ectenes is provided. Meanwhile, through assembly of the electric cell collecting device, the labor intensity is low, the practicability is high, the popularization value is large, the intensive and large-scale coilia ectenes seedling level can be greatly improved, and the coilia ectenes seedling industrialization progress is promoted.
Description
Technical field
The invention belongs to the numerous foster technical field of aquatic products, relate to a kind of observation and the collection separating method that produce the transparent fertilized egg of small-sized buoyant egg fish, the method that especially a kind of fertilized egg observation of important migration fishes-Coilia ectenes in Yangtze River and collection are separated.
Background technology
Enjoy the Changjiang river hairtail of one of " the Changjiang river three delicacies " good reputation, formal name used at school cutter long-tailed anchovy (
coilia nasus), with plump grossness, the fresh delicacy of meat and famous, is the important breeding migration fish of China, has high economic worth.The research of cutter long-tailed anchovy reproductive biology is just set about carrying out from the seventies by China, adopts the mode by catching ripe wild type to carry out artificial propagation test, because Coilia ectenes in Yangtze River gonad development colony poor synchronization, stress reaction are strong, does not have substantial progress always.In recent years, China Aquatic Science Research Academy Fresh Water Fishery Research Center together unit such as Jiangyin Shengang Sanxian Cultivation Co., Ltd., Jiangsu Zhongyang Group Co., Ltd. has carried out the tackling of key scientific and technical problems that exceedes 10 years round the key issue that cutter long-tailed anchovy in industry breeds, by the end of the year 2014, achieved and a series ofly bred relevant achievement in research, cutter long-tailed anchovy has become a kind of and has a extensive future, the high-quality breed variety of tool cultural connotation.
From 2009, after the patent of invention " a kind of whole artificial propagation method of cutter long-tailed anchovy " (patent No.: ZL200910034086.9) of China Aquatic Science Research Academy Fresh Water Fishery Research Center and Jiangyin Shengang Sanxian Cultivation Co., Ltd. discloses artificial induced spawning, test-tube propagation technique, the same year, patent of invention " a kind of impel the method for the Changjiang river hairtail natural spawning " (patent No.: ZL200910233173.7) of Liu Qinghua disclosed the propagation technique of artificial induced spawning, natural spawning; 2011, the patent of invention " a kind of Coilia ectenes in Yangtze River indoor intensive artificial raise seedling method " (patent No.: ZL201110303942.3) of Shanghai Aquatic Product Institute disclosed cutter long-tailed anchovy and adopts the methods such as artificial induced spawning to carry out the method for artificial propagation; Within 2014, Dalian Ocean University also reports cutter long-tailed anchovy artificial insemination success; Can say, attack through numerous unit the different mode that difficult gram of difficulty has established the artificial propagation of cutter long-tailed anchovy.But the problems such as cutter long-tailed anchovy stress reaction is strong, reproductive population gonad development is asynchronous annoying the artificial propagation of cutter long-tailed anchovy all the time, cause there is the indisputable fact that cutter long-tailed anchovy seedling cost is high, success rate is low always, for this reason, in recent years, numerous unit starts again the pattern exploring cutter long-tailed anchovy ecological breeding, the patent of invention " a kind of method of cutter long-tailed anchovy seedling rearing in earth ponds " of China Aquatic Science Research Academy Fresh Water Fishery Research Center in the 2013 (patent No.: 201310361937.7) disclose and impel cutter long-tailed anchovy natural spawning and the practical technique of cultivating with regard to pond by the regulation and control pond ecological factor, but still it is limited and cannot meet the seed demand of growing cutter long-tailed anchovy industry to there is nursery amount, therefore, nursery amount can be improved in the urgent need to one and can reuse again cutter long-tailed anchovy parent, the basis findding out pond and support cutter long-tailed anchovy egg-laying pattern utilizes the characteristic of this fertilized egg carry out Efficient Collection and be separated hatching, reach the target of cutter long-tailed anchovy scale breeding, meet the demand of industrial scale development tool setting long-tailed anchovy seed.
Summary of the invention
The technical problem to be solved in the present invention is, for the deficiency of existing cutter long-tailed anchovy artificial propagation techniques, makes up the deficiency that the nursery amount of current cutter long-tailed anchovy seedling rearing in earth ponds technology is limited, proposes a kind of pond and supports the observation of cutter long-tailed anchovy fertilized egg and collect the method be separated.
Pond is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that comprising the steps:
(1) the special cultivation pond of cutter long-tailed anchovy parent population is selected;
(2), between 5 to July, when surface temperature is more than 20 DEG C, fetch water in lower air port with beaker in night, then by the light source of glare light electric torch from lower on towards beaker bottom; During observation, naked eyes, from down upper, if observe shinny suspension fish-egg to be cutter long-tailed anchovy fertilized egg, can start to collect ovum;
(3), when collecting ovum, adopt water pump to draw water, bailing outlet is placed in below the water surface in lower air port, the mesh bag that pump outlet lock ring screen cloth is made;
(4), after starting water pump collection ovum 1-1.5h, the fertilized egg that mesh bag is collected is rinsed in bucket; Fertilized egg in bucket is soaked 10-15min with 1% gentian violet solution to carry out disinfection deinsectization, topple over the fertilized egg of isolating upper strata, proceed to hatching pail after then cleaning 3 times with clean aeration Spawning water and hatch.
It is further characterized in that: the time of observing fertilized egg in described step (2) is the 19:00-21:00 time period in the evening.
Preferred: in described step (2), glare light electric torch illuminance is at more than 10000Lx.
5-10cm below the water surface that water pump bailing outlet is placed in lower air port in described step (3); Described mesh bag is by long 5-8m, cross-sectional area 0.1-0.15m
280 eye mesh screens make, mesh bag tail end is bundled into slip-knot.
The present invention equips by simple and easy targetedly the egg-laying pattern that cutter long-tailed anchovy is supported in observation pond; drawn water by water pump, screen filtration, gentian violet solution-treated, the technological process such as cleaning; make to adapt to when not damaging cutter long-tailed anchovy parent population cutter long-tailed anchovy raun to lay eggs asynchronous, the little and transparent characteristic being difficult to observe, the duration of laying eggs is long of fish-egg, thus realize the large-scale production of cutter long-tailed anchovy seed.
Compared with the prior art the present invention has following beneficial effect:
1, according to the characteristic of cutter long-tailed anchovy fertilized egg; specific aim adopts the simple and easy equipment such as glass beaker, torch; quick judgement egg-laying time; solution naked eyes are directly difficult to observe cutter long-tailed anchovy fertilized egg must by the problem of sediments microscope inspection; for the breeding of cutter long-tailed anchovy provides simple and easy, practical technology, establish the basis of biological control.
2, practical, the promotional value of the special electronic collection ovum equipment of employing of the present invention is large, avoids manually fish for loaded down with trivial details, greatly can promote cutter long-tailed anchovy intensive seedling production level, promote cutter long-tailed anchovy nursery industry process.
Embodiment
Pond is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that comprising the steps:
(1) select area 2-5 mu, and have the special parent fish rearing pond of the cutter long-tailed anchovy of cement bank protection.
(2) between May 1 to July 31, when surface temperature is more than 20 DEG C, prepare simple and easy equipment 200mL or 500mL glass beaker, each 1 of high light hand carried electric torch (illuminance is at more than 10000Lx), select the 19:00-21:00 time period in the evening, fetch water 3/4 glass with beaker in lower air port, then by the light source of glare light electric torch from lower on towards beaker bottom; During observation, naked eyes, from down upper, if observe shinny suspension fish-egg to be cutter long-tailed anchovy fertilized egg, can start to collect ovum.
(3) when collecting ovum, 5-10cm below the water surface 6 inch water pump bailing outlets being placed in lower air port, the long 5-8m of pump outlet lock ring, cross-sectional area 0.1-0.15m
2the mesh bag made of 80 eye mesh screens, mesh bag tail end is bundled into slip-knot.
(4) after starting water pump collection ovum 1-1.5h, rinse cutter long-tailed anchovy fertilized egg in bucket, again can carry out collection ovum by slip-knot tie-down mesh bag tail end; Fertilized egg in bucket is soaked 10-15min with 1% gentian violet solution to carry out disinfection deinsectization, topple over the fertilized egg of isolating upper strata, proceed to hatching pail after then cleaning 3 times with clean aeration Spawning water and hatch.
Claims (5)
1. pond is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that comprising the steps:
(1) the special cultivation pond of cutter long-tailed anchovy parent population is selected;
(2), between 5 to July, when surface temperature is more than 20 DEG C, fetch water in lower air port with beaker in night, then by the light source of glare light electric torch from lower on towards beaker bottom; During observation, naked eyes, from down upper, if observe shinny suspension fish-egg to be cutter long-tailed anchovy fertilized egg, can start to collect ovum;
(3), when collecting ovum, adopt water pump to draw water, bailing outlet is placed in below the water surface in lower air port, the mesh bag that pump outlet lock ring screen cloth is made;
(4), after starting water pump collection ovum 1-1.5h, the fertilized egg that mesh bag is collected is rinsed in bucket; Fertilized egg in bucket is soaked 10-15min with 1% gentian violet solution to carry out disinfection deinsectization, topple over the fertilized egg of isolating upper strata, proceed to hatching pail after then cleaning 3 times with clean aeration Spawning water and hatch.
2. pond according to claim 1 is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that: the time of observing fertilized egg in described step (2) is the 19:00-21:00 time period in the evening.
3. pond according to claim 1 and 2 is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that: in described step (2), glare light electric torch illuminance is at more than 10000Lx.
4. pond according to claim 1 and 2 is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that: 5-10cm below the water surface that water pump bailing outlet is placed in lower air port in described step (3).
5. pond according to claim 1 and 2 is supported the observation of cutter long-tailed anchovy fertilized egg and is collected the method be separated, and it is characterized in that: described in described step (3), mesh bag is by long 5-8m, cross-sectional area 0.1-0.15m
280 eye mesh screens make, mesh bag tail end is bundled into slip-knot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510056409.XA CN104604772A (en) | 2015-02-04 | 2015-02-04 | Method for observing, collecting and separating germ cells of pond-cultured coilia ectenes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510056409.XA CN104604772A (en) | 2015-02-04 | 2015-02-04 | Method for observing, collecting and separating germ cells of pond-cultured coilia ectenes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104604772A true CN104604772A (en) | 2015-05-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510056409.XA Pending CN104604772A (en) | 2015-02-04 | 2015-02-04 | Method for observing, collecting and separating germ cells of pond-cultured coilia ectenes |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2564780Y (en) * | 2002-07-15 | 2003-08-06 | 厦门大学 | Pneumatic water pump |
| CN101669452A (en) * | 2009-06-30 | 2010-03-17 | 刘青华 | Mimic ecological propagation method for breeding parent fish of American hilsa herring |
| CN101692791A (en) * | 2009-08-21 | 2010-04-14 | 中国水产科学研究院淡水渔业研究中心 | Totally artificial breeding method for Coilia ectenes |
| CN101715747A (en) * | 2009-11-27 | 2010-06-02 | 刘青华 | Method for promoting Yangtze River saury to naturally spawn |
| CN103026988A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor intensive artificial breeding technique of Yangtze River Coilia ectenes |
| CN103404462A (en) * | 2013-08-20 | 2013-11-27 | 中国水产科学研究院淡水渔业研究中心 | Method for cultivating fries of coilia ectenes in soil pond |
| CN103891657A (en) * | 2014-04-14 | 2014-07-02 | 江苏中洋集团股份有限公司 | Natural propagation method for coilia ectenes |
-
2015
- 2015-02-04 CN CN201510056409.XA patent/CN104604772A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2564780Y (en) * | 2002-07-15 | 2003-08-06 | 厦门大学 | Pneumatic water pump |
| CN101669452A (en) * | 2009-06-30 | 2010-03-17 | 刘青华 | Mimic ecological propagation method for breeding parent fish of American hilsa herring |
| CN101692791A (en) * | 2009-08-21 | 2010-04-14 | 中国水产科学研究院淡水渔业研究中心 | Totally artificial breeding method for Coilia ectenes |
| CN101715747A (en) * | 2009-11-27 | 2010-06-02 | 刘青华 | Method for promoting Yangtze River saury to naturally spawn |
| CN103026988A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor intensive artificial breeding technique of Yangtze River Coilia ectenes |
| CN103404462A (en) * | 2013-08-20 | 2013-11-27 | 中国水产科学研究院淡水渔业研究中心 | Method for cultivating fries of coilia ectenes in soil pond |
| CN103891657A (en) * | 2014-04-14 | 2014-07-02 | 江苏中洋集团股份有限公司 | Natural propagation method for coilia ectenes |
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Application publication date: 20150513 |