CN104585374A - Preparation method and application of tea containing salidroside - Google Patents
Preparation method and application of tea containing salidroside Download PDFInfo
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- CN104585374A CN104585374A CN201510004859.4A CN201510004859A CN104585374A CN 104585374 A CN104585374 A CN 104585374A CN 201510004859 A CN201510004859 A CN 201510004859A CN 104585374 A CN104585374 A CN 104585374A
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Abstract
The invention provides a preparation method of tea containing salidroside. The method comprises the following steps: spraying p-hydroxyphenylethyl alcohol to growing camellia sinensis leaves, and picking to obtain the tea containing salidroside. The method provided by the invention is simple in step; a synthetic precursor, namely p-hydroxyphenylethyl alcohol, of the salidroside is directly supplied from the external world; and uridine diphosphate glucose and glucosyltransferase contained in the tea are taken as raw materials, and the salidroside is synthesized in a tea body. The prepared tea containing the salidroside and application of the tea are further protected in the invention.
Description
Technical field
The present invention relates to technical field of biotransformation, relate to a kind of preparation method of the tealeaves containing rhodioside particularly.
Background technology
Rhodioside (salidroside) is the most important effective component of Crassulaceae (Crassulaceae) rhodiola (Rhodiola L.) plant, have that antifatigue, develop immunitypty, protection are cardiovascular, anti anoxia, anti-ageing, neuroprotective cell, protection liver, the multiple special effect such as anti-oxidant, antitumor, radioresistance.In military affairs, space flight, motion and physical-fitness medicine etc., there is extremely important using value.In recent years, get more and more using it as the kind of the products such as the medicament of main material production, beverage, food and cosmetics, people to the demand of rhodioside in continuous increase.
Rhodida plant is the main source extracting rhodioside, but wild resource is deficient, and Determination of Salidroside is very low, and as the most frequently used sachalin rhodiola rhizome and rhodiola, in its plant, the content of rhodioside only has 0.5% ~ 0.8%.Although achieve progress at present in artificial growth, but because rhodiola root kernel maturing is poor, germination percentage is low, and need grow in the suitable High aititude mountain area of environment, tame rhodiola root cost is higher and active constituent content is low, is still difficult to a large amount of supplies ensureing rhodiola root raw material.
The biosynthesis of occurring in nature rhodioside is with tyrosol (i.e. p-hydroxyphenylethanol) for substrate, by the glucosyltransferase catalysis in plant, shifts a glucosyl group on tyrosol, forms rhodioside.But occurring in nature most plants all can not synthesize tyrosol, thus not containing rhodioside.
The synthetic method of rhodioside mainly contains Plant Tissue Breeding, culture plant cell, biological enzyme and chemical method synthesis.What wherein tissue cultures and cell chulture adopted is all Rhodida plants, by methods such as Optimal Medium condition and interpolation tyrosols, improves output and yield.As the people such as Han Aiming are optimized the culture medium in sachalin rhodiola rhizome cell suspension cultures process, in stem cell, Determination of Salidroside is 5.66mg/g, repeat 3 times in the exponential phase of growth of rhodiola root suspended culture cell every 24h in addition and add 3mmol/L tyrosol, the output of rhodioside can reach 516 μm of ol/L.Adopt tissue cultures and cell chulture to produce rhodioside, because the cycle is long, cost is high, obtains large-scale application not yet aborning.
Rhodioside is a kind of glucosides be combined into glycosidic bond by glucose and tyrosol, and tyrosol is its aglycon, and enzyme process can be adopted to synthesize.Bibliographical information is had to mention, the beta-glycosidase synthesis rhodioside extracted in vegetable seeds can be utilized, the beta-glycosidase such as obtained from Apple Seeds, can make 4-substituted benzyl alcohol and rhodiola root aglycon and glucose, in water-diox single-phase medium, glycosylation occur, glucosides productive rate about 13.1 ~ 23.1%, wherein rhodioside about 15.8%.But beta-glycosidase Main Function is in vivo the hydrolysis of catalysis glycosidic bond, what synthesis rhodioside utilized is its back reaction, and this reaction limits by balance controlled and molecular balance, and yield is lower.Rhodioside also useful chemical method synthesis, but because chemical synthesis process need is through the protection of multistep with go safeguard measure, process is complicated and cost is high, accessory substance is more and be difficult to be separated, comparatively large to environmental hazard, therefore has no large-scale production.
In sum, the synthetic method of current rhodioside, due to reasons such as production cost are high, yield poorly, synthesis step is complicated, is showed no and industrially applies.Specified raw material is added about in the water body or soil of plant growth although have in prior art, the report generating rhodioside is transformed by biology, but this addition manner needs the material Real-Time Monitoring in water body or soil and constantly supplements, complicated operation, and a large amount of material loss added is many, major part cannot be absorbed, and transformation efficiency is not high, and have certain toxic action to root system of plant, affect root growth.
Tea tree belongs to Theaceae Camellia, is perennial evergreen xylophyta, and be important economic plants, people pluck its tender leaf or tender shoots usually, after processing, make tealeaves.Due to the difference of processing mode, the kinds such as green tea, black tea, oolong tea, black tea can be divided into again, but usually all containing the composition such as Tea Polyphenols, caffeine, there is stronger excitation.In the tea free of nature spontaneous growth, do not find containing rhodioside composition.At present, there is not yet the relevant report that the health tealeaves containing rhodioside is produced in preparation.Along with people are for the continuous pursuit of healthy living, adopt the method for safety to synthesize rhodioside in tealeaves, prepare the tealeaves containing rhodioside, there is good development and application prospect.
Summary of the invention
The object of the present invention is to provide a kind of easy, efficient, safe preparation containing the method for the tealeaves of rhodioside.
The invention provides a kind of preparation method of the tealeaves containing rhodioside, said method comprising the steps of: the blade to the tea tree of growth sprays p-hydroxyphenylethanol, plucks blade, is processed into tealeaves, obtains the tealeaves containing rhodioside.
Tea tree of the present invention (Classification system Camellia sinensis) belongs to Theaceae tea tree and belongs to, and the blade of described tea tree, comprises the leaf of tea tree or/and bud.
The described tea tree be sprayed should be in growth phase, and the initial time of sprinkling is preferably tea leaf and normally plucks random time within the scope of first 1 ~ 2 month.
Described processing method is the tea Processing method of this area routine; Described tealeaves comprises green tea, black tea, white tea, yellow tea, black tea, blue or green tea etc.
Described p-hydroxyphenylethanol, take p-hydroxyphenylethanol as the solution of the formulated concentration 0.01 ~ 10% of solute, emulsion or suspension, be preferably the solution of concentration 0.1 ~ 5%, emulsion or suspension, more preferably the solution of concentration 0.2 ~ 2%, emulsion or suspension; Described concentration is the mass volume ratio of solute and solvent, and the implication of percentage is g/100ml.
The number of times of described sprinkling can be 1 time, also can be repeatedly, and described is repeatedly 2 ~ 3 times; When spraying number of times and being greater than 1 time, the time interval of spraying for each time is 2 ~ 30 days, is preferably 2 ~ 15 days, more preferably 2 ~ 5 days.
The time of described harvesting, for spraying latter 2nd ~ 15 days, is preferably 2 ~ 7 days, more preferably 2 ~ 3 days; When spraying number of times and being greater than 1 time, described plucking time is the number of days after last sprinkling.
Concrete, method provided by the invention comprises the following steps: to the p-hydroxyphenylethanol solution of tea leaf spray concentration 0.01 ~ 10% of growth, emulsion or suspension, within after spraying 2nd ~ 15 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
Preferably, method provided by the invention comprises the following steps: the tea leaf to growth sprays the p-hydroxyphenylethanol solution of 2 ~ 3 concentration 0.01 ~ 10%, emulsion or suspension, the time interval of spraying for each time is 2 ~ 30 days, and last sprinkling is plucked for latter 2 ~ 15 days, to obtain final product.
More preferably, method provided by the invention comprises the following steps: the tea leaf to growth sprays the p-hydroxyphenylethanol solution of 2 ~ 3 concentration 0.1 ~ 5%, emulsion or suspension, the time interval of spraying for each time is 2 ~ 15 days, within after last sprinkling 2 ~ 7 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
Further preferably, method provided by the invention comprises the following steps: the tea leaf to growth sprays the p-hydroxyphenylethanol solution of 2 ~ 3 concentration 0.2 ~ 2%, the time interval of spraying for each time is 2 ~ 5 days, within after last sprinkling 2 ~ 3 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
As a kind of preferred version, method provided by the invention comprises the following steps: the tea leaf to growth sprays the p-hydroxyphenylethanol aqueous solution of 3 concentration 0.2%, the time interval of spraying for each time is 2 days, last sprinkling plucks blade in latter 3 days, be processed into tealeaves, obtain the tealeaves containing rhodioside.
Sprinkling of the present invention should make p-hydroxyphenylethanol solution, emulsion or suspension cover tea leaf surface.
The solvent of p-hydroxyphenylethanol of the present invention can be water, any one oil or organic solvent, or the mixed solvent that these solvents form by a certain percentage, or the solvent be made into by these solvents and acid, alkali, salt, surfactant etc.
P-hydroxyphenylethanol of the present invention can be replaced by one or more in Uteramin or p-hydroxyphenylaceticacid.Described Uteramin, p-hydroxyphenylaceticacid or para hydroxybenzene acetaldehyde are sprayed onto blade surface, and then after being absorbed by tealeaves, under the effect of relevant enzyme, can be converted into p-hydroxyphenylethanol and participate in subsequent reactions.When described Uteramin or p-hydroxyphenylaceticacid concentration higher time, dissolubility is poor, need add cosolvent, forms emulsion or suspension.
Method provided by the invention is based on following reaction principle: with the uridine diphosphoglucose (UDPG) contained by tea leaf itself for substrate, utilize the glucosyltransferase that tea leaf itself is contained, glucosyl group on uridine diphosphoglucose is transferred on p-hydroxyphenylethanol, thus the p-hydroxyphenylethanol of external source is changed into rhodioside.Described conversion can naturally-occurring in tea tree body, and without the need to applying other catalytic specie or changing external condition, and the p-hydroxyphenylethanol of external source is substantially noiseless to the normal growth of tea tree.Above-mentioned mechanism can represent with following reaction equation:
The present invention finds to be found by great many of experiments, and by p-hydroxyphenylethanol is sprayed at tea leaf on the surface, p-hydroxyphenylethanol absorption enters in body by blade by tea tree, reacts under the effect of glycosyl transferase with UDPG, synthesis rhodioside.Due to the activity form that UDPG is glucose, the cell of tea leaf itself constantly can produce UDPG, because the synthesis of the glucides such as starch all needs the participation of UDPG, therefore UDPG content and distribution can not become the biosynthetic limiting factor of rhodioside of the present invention.
In its natural state, rhodioside is not contained in tea tree body.P-hydroxyphenylethanol is sprayed at this easy, efficient mode of operation of tea leaf surface by method choice provided by the invention, and the preferred concentration of p-hydroxyphenylethanol and the series of parameters such as time, number of times, interval of applying, because the synthesis precursor p-hydroxyphenylethanol of rhodioside is directly supplied by the external world, the uridine diphosphoglucose that tea tree itself therefore can be utilized contained and glucosyltransferase are raw material, in tea tree body, synthesize rhodioside, described method safety is reliable.
The tealeaves that the present invention protects described method to prepare.
The application of the tealeaves that the present invention protects described method to prepare further, comprises with described tealeaves for food materials directly eat, or extracts rhodioside as raw material or prepare health food, food additives, medicine etc.
The invention provides very easy, the safety of technical scheme operation, compared with prior art there is significant progress.Method provided by the invention, p-hydroxyphenylethanol is sprayed directly on the blade surface of tea tree, with add the technical scheme of p-hydroxyphenylethanol in the water body or soil of tea growth compared with, foliage-spray has significant advantage, easy to operate, can spray at any time, and greatly reduce the use amount of p-hydroxyphenylethanol, loss is few, if adopt the mode added in tea tree incubation in nutrient solution or soil, then need to monitor in real time the concentration of p-hydroxyphenylethanol, continuous interpolation, and p-hydroxyphenylethanol loss is many, major part cannot be absorbed, in addition, p-hydroxyphenylethanols etc. have certain toxic action to root system of plant, affect root growth, and pass through Foliage Absorption, growth of tea plant is had no adverse effect substantially.The tea tree producing rhodioside is difficult under adopting method of the present invention can make natural conditions; can transform in vivo and accumulate rhodioside; thus the physiological and pharmacological that acquisition rhodioside has is active; the effect such as cardiovascular in antifatigue, develop immunitypty, protection, anti anoxia, anti-ageing, neuroprotective cell, protection liver, anti-oxidant, antitumor, radioresistance; after blade processing is become tealeaves; can be used as tealeaves directly to eat, also can be used as the raw material that rhodioside extracts.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
1, preparation method: the blade to the tea tree of growth sprays the 0.5% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside for after spraying the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: adopt efficient liquid-phase chromatography method, get 5g embodiment and control group gained green tea respectively, as two groups of testing samples, add 50% methyl alcohol 25ml, weighed weight, adds hot reflux 30 minutes, lets cool, supply the weight of less loss, filter, as need testing solution.Separately get rhodioside standard items, add 50% methyl alcohol and make the solution containing 0.05mg in every 1ml, as standard solution.Be filler with octadecylsilane chemically bonded silica; Methanol-water (12:88) is mobile phase; Determined wavelength is 275nm.Number of theoretical plate calculates should be not less than 3000 by rhodioside peak.Accurate absorption need testing solution and each 10 μ l of reference substance solution respectively, injection liquid chromatography, measures.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.031%, and control group can't detect rhodioside.
Embodiment 2:
1, preparation method: the blade to the tea tree of growth sprays the 10% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside on the 15th day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.014%, and control group can't detect rhodioside.
Embodiment 3:
1, preparation method: the blade to the tea tree of growth sprays the 5% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside on the 7th day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.035%, and control group can't detect rhodioside.
Embodiment 4:
1, preparation method: the blade to the tea tree of growth sprays the 2% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside on the 3rd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.042%, and control group can't detect rhodioside.
Embodiment 5:
1, preparation method: the blade to the tea tree of growth sprays the 0.1% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside on the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.015%.
Embodiment 6:
1, preparation method: the blade to the tea tree of growth sprays the 0.01% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside on the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.005%, and control group can't detect rhodioside.
Embodiment 7:
1, preparation method: the blade spray concentration to the tea tree of growth is the 5% p-hydroxyphenylethanol aqueous solution, sprays once every 30 days again, second time plucks blade on the 4th day after spraying, and is conventionally processed into green tea, obtains the green tea containing rhodioside;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the present embodiment gained green tea, Determination of Salidroside is 0.045%, and control group can't detect rhodioside.
Embodiment 8:
1, preparation method: the blade spray concentration to the tea tree of growth is the 2% p-hydroxyphenylethanol aqueous solution, sprays once every 15 days again, second time plucks blade on the 3rd day after spraying, and is conventionally processed into black tea, obtains the black tea containing rhodioside;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained black tea, Determination of Salidroside is 0.046%, and control group can't detect rhodioside.
Embodiment 9:
1, preparation method: the blade to the tea tree of growth sprays the 0.8% p-hydroxyphenylethanol aqueous solution, sprays once every 5 days again, second time plucks blade on the 2nd day after spraying, and is conventionally processed into black tea, obtains the black tea containing rhodioside;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained black tea, Determination of Salidroside is 0.048%, and control group can't detect rhodioside.
Embodiment 10:
1, preparation method: the blade to the tea tree of growth sprays the 0.2% p-hydroxyphenylethanol aqueous solution, sprays once every 3 days again, second time plucks blade on the 2nd day after spraying, and is conventionally processed into black tea, obtains the black tea containing rhodioside;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained black tea, Determination of Salidroside is 0.041%, and control group can't detect rhodioside.
Embodiment 11:
1, preparation method: the blade to the tea tree of growth sprays 3 0.2% p-hydroxyphenylethanol aqueous solution, sprays interval 2 days each time, and last sprinkling plucks blade in latter 3rd day, is conventionally processed into green tea, obtains the green tea containing rhodioside;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained green tea, Determination of Salidroside is 0.051%, and control group can't detect rhodioside.
Embodiment 12:
1, preparation method: the blade to the tea tree of growth sprays the ethanolic solution (solvent is 10% ethanol) of 0.6% p-hydroxyphenylethanol, plucks blade, is conventionally processed into black tea, obtains the black tea containing rhodioside for after spraying the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the black tea of embodiment gained, Determination of Salidroside is 0.040%, and control group can't detect rhodioside.
Embodiment 13:
1, preparation method: the blade to the tea tree of growth sprays the 10% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into black tea, obtains the black tea containing rhodioside for after spraying the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in the black tea of embodiment gained, Determination of Salidroside is 0.048%, and control group can't detect rhodioside.
Embodiment 14:
1, preparation method: the blade to the tea tree of growth sprays the 0.8% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside for after spraying the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained green tea, Determination of Salidroside is 0.045%, and control group can't detect rhodioside.
Embodiment 15:
1, preparation method: the blade to the tea tree of growth sprays the 0.2% p-hydroxyphenylethanol aqueous solution, plucks blade, is conventionally processed into green tea, obtains the green tea containing rhodioside for after spraying the 2nd day;
Arrange control group, the difference of control group and the present embodiment is only simultaneously, not containing p-hydroxyphenylethanol in the solvent that blade sprays.
2, the detection of rhodioside: detection method is with embodiment 1.
From testing result, in embodiment gained green tea, Determination of Salidroside is 0.025%, and control group can't detect rhodioside.
Embodiment 16: directly eat
Green tea in Example 11 eats.
The health care that gained green tea has that antifatigue, develop immunitypty, protection are cardiovascular, anti anoxia, anti-ageing, neuroprotective cell, protection liver, anti-oxidant, antitumor, radioresistance etc. have based on rhodioside.
Embodiment 17: extract rhodioside
The green tea that embodiment 11 obtains is extracted raw material as rhodioside.
Extraction step is: by tealeaves, add water 6 times amount, decoct 1 hour, filter, it is 1.25 that filtrate is concentrated into relative density, let cool, with equal-volume extracting n-butyl alcohol 2 times, merge butanol extraction liquid, be evaporated to without alcohol taste, after adding 3 times of water gaging dilutions, with 1BV (bed volume)/h by AB-8 large pore resin absorption column, first use the water washing of 3 times of bed volumes, discard, 10% ethanol washing of 2 times of bed volumes again, discard, use 5 times of bed volume 20% ethanol elutions again, collect eluent, be evaporated to dry, obtain CE, add after methyl alcohol dissolves in right amount, add the silica gel of 3 times of CE weight, mixing, add the silicagel column top of 20 times of CE weight, with 10 times of bed volume methanol-chloroform (2:8) solution, carry out wash-out, collect eluent, evaporated under reduced pressure, with absolute ethyl alcohol crystallization below 10 DEG C of 10 times of volumes, magma centrifugation, after absolute ethanol washing, vacuum drying, obtaining purity is the rhodioside of 98.6%, yield is 21.3%.
Embodiment 18: preparation is containing rhodioside tea drink
By the green tea that embodiment 11 obtains, as the raw material of preparation health drink.
Example 11 gained green tea 1kg, adds 10 times of deionized waters, 80 DEG C of lixiviates 5 minutes, filters, and add deionized water dilution 2 to 5 times, add 1% ascorbic acid, 120 DEG C of sterilizings in 7 minutes, packing, makes tea beverage.
Gained tea beverage has relieving sports fatigue, develop immunitypty, raising anoxia endurance, anti-oxidant and assistant protection function to hepatic injury.
Embodiment 19: the food additives of preparation containing rhodioside
Add water the green tea that above-described embodiment 11 obtains 8 times amount, decocts 1 hour, and decocting liquid filters, after filtrate is condensed into thick paste, get dry extract in 60 DEG C of vacuum drying, namely can be used as food additives after pulverizing and use, add in beverage, beverage can be made to have the health care of rhodioside relieving fatigue.
Embodiment 20: the medicine of preparation containing rhodioside
The green tea boiling that above-described embodiment 11 is obtained 2 times, add water 8 times amount at every turn, decocts 1 hour, and decocting liquid merges filtration, after filtrate is condensed into thick paste, get dry extract, after pulverizing in 60 DEG C of vacuum drying, get dried cream powder 300g, add medical starch 50g, pharmaceutical technology makes tablet routinely.
Gained medicine is used for the treatment of and prevents altitude sickness, or is used for the treatment of coronary heart disease, cerebral infarction, the diseases such as senile dementia.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. containing a preparation method for the tealeaves of rhodioside, it is characterized in that, comprise the following steps: the blade to the tea tree of growth sprays p-hydroxyphenylethanol, plucks blade, is processed into tealeaves, obtains the tealeaves containing rhodioside.
2. method according to claim 1, is characterized in that, described p-hydroxyphenylethanol is the p-hydroxyphenylethanol solution of concentration 0.01 ~ 10%, emulsion or suspension; Preferred concentration is 0.1 ~ 5%; Further preferred concentration is 0.2 ~ 2%.
3. method according to claim 1, is characterized in that, the time of described harvesting is for spraying latter 2 ~ 15 days; Be preferably 2 ~ 7 days; More preferably 2 ~ 3 days.
4. method according to claim 1, it is characterized in that, said method comprising the steps of: to the p-hydroxyphenylethanol solution of tea leaf spray concentration 0.01 ~ 10% of growth, emulsion or suspension, within after spraying 2nd ~ 15 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
5. the method according to Claims 1 to 4 any one, is characterized in that, described sprinkling is for repeatedly to spray, and spraying number of times is 2 ~ 3 times.
6. method according to claim 5, is characterized in that, the described time interval of repeatedly spraying is 2 ~ 30 days, is preferably 2 ~ 15 days, more preferably 2 ~ 5 days.
7. method according to claim 6, it is characterized in that, said method comprising the steps of: the tea leaf to growth sprays the p-hydroxyphenylethanol solution of 2 ~ 3 concentration 0.1 ~ 5%, emulsion or suspension, the time interval of spraying for each time is 2 ~ 15 days, within after last sprinkling 2 ~ 7 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
8. method according to claim 7, it is characterized in that, said method comprising the steps of: the tea leaf to growth sprays the p-hydroxyphenylethanol solution of 2 ~ 3 concentration 0.2 ~ 2%, the time interval of spraying for each time is 2 ~ 5 days, within after last sprinkling 2 ~ 3 days, pluck blade, be processed into tealeaves, obtain the tealeaves containing rhodioside.
9. method described in claim 1 ~ 8 any one be prepared from containing the tealeaves of rhodioside.
10. contain the application of the tealeaves of rhodioside described in claim 9, it is characterized in that, described tealeaves directly eats as food materials, or extracts rhodioside as raw material, or prepares health food, food additives, medicine as raw material.
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| CN104878025A (en) * | 2015-06-01 | 2015-09-02 | 安徽农业大学 | Galloyl glucosyltransferase CsUGT84A22 gene and encoded protein thereof and application |
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| CN104878025B (en) * | 2015-06-01 | 2017-11-03 | 安徽农业大学 | A kind of galloyl glucose based transferase CsUGT84A22 genes and its encoding proteins and application |
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