CN103999648B - A kind of cultivation method containing salidroside plant and plant application - Google Patents
A kind of cultivation method containing salidroside plant and plant application Download PDFInfo
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- CN103999648B CN103999648B CN201310061826.4A CN201310061826A CN103999648B CN 103999648 B CN103999648 B CN 103999648B CN 201310061826 A CN201310061826 A CN 201310061826A CN 103999648 B CN103999648 B CN 103999648B
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Abstract
The invention provides a kind of cultivation method containing salidroside plant and plant application, tyrosol is added in the solid dielectric such as soil, matrix, fertilizer of plant growth, per kilogram solid dielectric adds 0.1 ~ 100g tyrosol, or add 0.1 ~ 1000g tyrosol for every strain plant, at interval of certain hour, supplement and add tyrosol once, also can not supplement.After plant absorption tyrosol, in cell relevant enzyme effect under be converted into rhodioside, thus it is active to make plant obtain the physiological and pharmacological that has of rhodioside, can directly use, also can be made into health food, medicine, food additives etc., or as the raw material that rhodioside extracts.
Description
Technical field
The present invention relates to technical field of biotransformation, especially, relate to a kind of cultivation method containing salidroside plant and plant application.
Background technology
Rhodioside (salidroside) is the most important effective component of Crassulaceae (Crassulaceae) rhodiola (Rhodiola L.) plant, have that antifatigue, develop immunitypty, protection are cardiovascular, anti anoxia, anti-ageing, neuroprotective cell, protection liver, the multiple special effect such as anti-oxidant, antitumor, radioresistance.In military affairs, space flight, motion and physical-fitness medicine etc., there is extremely important using value.In recent years, get more and more using it as the kind of the products such as the medicament of main material production, beverage, food and cosmetics, people to the demand of rhodioside in continuous increase.
Rhodida plant is the main source extracting rhodioside, but wild resource is deficient, and Determination of Salidroside is very low, and as the most frequently used sachalin rhodiola rhizome and rhodiola, in its plant, the content of rhodioside only has 0.5% ~ 0.8%.Although achieve progress at present in artificial cultivation, but because rhodiola root kernel maturing is poor, germination rate is low, and need grow in the suitable High aititude mountain area of environment, tame rhodiola root cost is higher and active constituent content is low, is still difficult to a large amount of supplies ensureing rhodiola root raw material.
The biosynthesis of occurring in nature rhodioside is with tyrosol (i.e. p-hydroxyphenylethanol) for substrate, by the glucosyltransferase catalysis in plant corpus, shifts a glucosyl group on tyrosol, forms rhodioside.But occurring in nature most plants all can not synthesize tyrosol, thus not containing rhodioside.
The synthetic method of rhodioside mainly contains Plant Tissue Breeding, culture plant cell, biological enzyme and chemical method synthesis.What wherein tissue cultures and cell chulture adopted is all Rhodida plants, by methods such as Optimal Medium condition and interpolation tyrosols, improves output and yield.As the people such as Han Aiming are optimized the medium in sachalin rhodiola rhizome cell suspension cultures process, in stem cell, Determination of Salidroside is 5.66mg/g, repeat 3 times in the exponential phase of growth of rhodiola root suspended culture cell every 24h in addition and add 3mmol/L tyrosol, the output of rhodioside can reach 516 μm of ol/L.Adopt tissue cultures and cell chulture to produce rhodioside, because the cycle is long, cost is high, obtains large-scale application not yet aborning.
Rhodioside is a kind of glucosides be combined into glycosidic bond by glucose and tyrosol, and tyrosol is its aglycon, and enzyme process can be adopted to synthesize.Bibliographical information is had to mention, the beta-glycosidase synthesis rhodioside extracted in plant seed can be utilized, the beta-glycosidase such as obtained from Apple Seeds, can make 4-substituted benzyl alcohol and rhodiola root aglycon and glucose, in water-diox single-phase medium, glycosylation occur, glucosides productive rate about 13.1 ~ 23.1%, wherein rhodioside about 15.8%.But beta-glycosidase Main Function is in vivo the hydrolysis of catalysis glycosidic bond, what synthesis rhodioside utilized is its reverse reaction, and this reaction limits by balance controlled and molecular balance, and yield is lower.
Patent 201110005088.2 utilizes goldbeater's skin bright string strain bacterium glucosyltransferase in aqueous phase, synthesize rhodioside or analog, but first needs by the glucosyltransferase of fermentation mode production also needed for separation and purification, and the cost of enzyme is higher.
Rhodioside also useful chemical method synthesis, but because chemosynthesis process need is through the protection of multistep with go safeguard measure, process is complicated and cost is high, accessory substance is more and be difficult to be separated, comparatively large to environmental hazard, therefore has no large-scale production.
In sum, the synthetic method of current rhodioside, due to reasons such as production cost are high, yield poorly, synthesis step is complicated, is showed no and industrially applies.
Summary of the invention
The object of the invention is to provide a kind of cultivation method containing salidroside plant and plant application, the shortage of resources problem that existing rhodioside yields poorly to solve, chemical synthesis process complexity is brought.
For achieving the above object, the invention provides a kind of cultivation method containing salidroside plant: in the solid dielectric (soil, matrix or fertilizer etc.) of plant growth, add tyrosol, per kilogram solid dielectric adds 0.1 ~ 100g tyrosol, or every strain plant adds 0.1 ~ 1000g tyrosol.Plant just can transform tyrosol synthesis rhodioside in process of growth, after tyrosol is by plant consumption, repeatedly can supplements and add tyrosol, thus turn out the plant of being rich in rhodioside.Except can as extracting except the raw material of rhodioside, it is active that this plant and extract thereof have the physiological and pharmacological that rhodioside brings, and directly can use, also can be prepared into health food, food additives or medicinal application.
Preferably, can at interval of 1 ~ 100 day, supplement and add tyrosol, tyrosol addition is per kilogram solid dielectric 0.1 ~ 100g, or the tyrosol addition of every strain plant is 0.1 ~ 1000g.
Preferably, in the early stage of plant growth, at interval of 7 ~ 100 days, can add tyrosol 1 time, in the later stage of plant growth, at interval of 1 ~ 15 day, add tyrosol 1 time, tyrosol addition is per kilogram solid dielectric 0.1 ~ 100g, or is every strain plant 0.1 ~ 1000g.
Preferably, before plant harvest 1 ~ 15 day, stop adding tyrosol.
Preferably, tyrosol addition is per kilogram solid dielectric 0.1 ~ 50g, or the tyrosol addition of every strain plant is 0.1 ~ 500g.
The invention also discloses directly edible, the application of to extract raw material as rhodioside, there is for the preparation of health food or food additives, preparation rhodioside pharmacodynamic feature medicine of the plant that said method cultivates.
The enzyme that the present invention utilizes plant itself contained, mainly glucosyltransferase, aglycon---tyrosol synthesizes rhodioside in conversion.
→
In reaction equation, UDPG is uridine diphosphoglucose, and being glucose high energy activity form in vivo, is the precursor of synthesis of glucose glycosides.
Applicant finds through test of many times, and the glucosyltransferase in plant corpus can carry out catalysis to multiple aldehydes matter, obtains corresponding glucosides.And the species specificity of the glucosyltransferase in plant cell is also strong, the hair shape root etc. as the suspended culture cell of datura alba Nees, datura and ginseng all can transform p-Hydroxybenzylalcohol and generate corresponding glucosides.
Based on glucosyltransferase ubiquity and species specificity is not strong in plant, applicant finds through lot of experiments, by supplying ectogenic tyrosol, various plants all can transform tyrosol to synthesize rhodioside in process of growth, and accumulates in vivo.
Add ectogenic tyrosol in the soil, matrix, fertilizer etc. of plant growth after, plant can be absorbed to enter in body, in cell relevant enzyme effect under Synthesis be rhodioside, and the Different Organs in plant corpus, as the positions such as leaf, root, stem, flower, fruit accumulate.
The present invention has following beneficial effect:
The plant producing rhodioside is difficult under adopting method of the present invention can make natural conditions; can transform in vivo and accumulate rhodioside; thus the physiological and pharmacological that acquisition rhodioside has is active; the effect such as cardiovascular in antifatigue, develop immunitypty, protection, anti anoxia, anti-ageing, neuroprotective cell, protection liver, anti-oxidant, antitumor, radioresistance; can directly use; also can be made into the additive etc. of health food, medicine, food or cosmetics, during content height, also can be used as the raw material that rhodioside extracts.
The plant of rhodioside can be produced under natural conditions, as Rhodida plant, after adopting the method, its Determination of Salidroside can be made significantly to increase, increase its effect.
The transformation efficiency of plant on tyrosol is subject to the impact of many factors, wherein the amount of growth of plant itself has the greatest impact, also be subject to concentration and the composition of the nutrient component in soil, matrix, fertilizer simultaneously, the loss of tyrosol, sunshine length, environmental temperature, harmful substance, the many factors such as damage by disease and insect impact.The application can according to the change of plant growth amount and external environment, adjustment tyrosol addition in time, and the raw materials requirement providing enough tyrosols to meet rhodioside to transform, makes the transformation efficiency of rhodioside reach maximum, to obtain higher Determination of Salidroside.
Simultaneously also can as required, the amount that flexible adjustment tyrosol adds and number of times, turn out the plant of Different Red red-spotted stonecrop content, to meet different purposes.
Except object described above, feature and advantage, the present invention also has other object, feature and advantage.The present invention is further detailed explanation below.
Embodiment
Below embodiments of the invention are described in detail, but the multitude of different ways that the present invention can limit according to claim and cover is implemented.
The invention discloses a kind of cultivation method containing salidroside plant, comprise step:
In the solid dielectric (soil, matrix or fertilizer etc.) of plant growth, add tyrosol, per kilogram adds 0.1 ~ 100g tyrosol, or every strain plant adds 0.1 ~ 1000g tyrosol.Exogenous tyrosol absorbs and enters in body by plant, is converted into rhodioside.Its mesostroma refers to the sand, chad, vermiculite, rock wool, wood chip, bark, peat etc. that use in plant cultivation.
Tyrosol can add in solid form, also adds in water soluble.
In addition, when tyrosol be consumed a certain amount of after, can supplement and add tyrosol, with provide enough tyrosols meet rhodioside transform raw materials requirement.According to the amount of growth of plant, in the early stage (namely growing true leaf to start to before there is bud) of plant growth, can at interval of 7 ~ 100 days, supplement and add tyrosol 1 time, along with the increase of plant growth amount, the raising of tyrosol transformation efficiency, interval time can be shortened gradually or increase tyrosol content, in the later stage (namely occur bud the period to fruit maturation) of plant growth, can interval 1 ~ 15 day, supplement and add tyrosol 1 time.
Results before 1 ~ 15 day, can stop adding tyrosol, to allow plant all be transformed by remaining tyrosol.
Specific embodiment is as follows:
Embodiment one, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by per kilogram soil in the soil of transplanting 10g tyrosol (also can by tyrosol by water-soluble solution after add and bury, mixing), every 3 ~ 15 days, (initial stage was spaced apart 15 days, shorten interval time to 3 day gradually subsequently) apply containing the water of tyrosol in soil, also can mix in fertilizer and apply, addition is per kilogram soil 10g tyrosol, gather in the crops and within first 3 days, stop adding tyrosol, to allow tyrosol transform completely, romaine lettuce after results, measure Determination of Salidroside as stated above, its content (fresh weight) reaches as high as more than 0.15%.
Rhodioside detection method: adopt efficient liquid-phase chromatography method, get 0.5g testing sample, add 50% methyl alcohol 25ml, weighed weight, add hot reflux 30 minutes, let cool, supply the weight of less loss, filters, as need testing solution.Separately get rhodioside reference substance, add 50% methyl alcohol and make the solution containing 0.05mg in every 1ml, product solution in contrast.Be filler with octadecylsilane chemically bonded silica; Methanol-water (13:87) is mobile phase; Determined wavelength is 275nm.Number of theoretical plate calculates should be not less than 3000 by rhodioside peak.Accurate absorption need testing solution and each 10 μ l of reference substance solution respectively, injection liquid chromatography, measures, and calculates, to obtain final product.Following measuring method roughly the same, repeats no more.
Embodiment two, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 0.1g tyrosol (can by tyrosol by water-soluble solution after add and bury, mixing), in the soil of its growth, the water containing tyrosol was applied every 5 days after transplanting, tyrosol addition at every turn for previous 2 times (be namely respectively per kilogram soil 0.2,0.4,0.8,1.6,3.2,6.4,12.8g, after reaching 12.8g, concentration no longer increases, and is 12.8g later at every turn).Gather in the crops and within first 5 days, stop adding tyrosol, to allow tyrosol transform completely, the romaine lettuce after results, measures Determination of Salidroside as stated above, and its content (fresh weight) reaches as high as more than 0.18%.
Embodiment three, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 2g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), in soil, the water containing tyrosol is applied every 7 days, also can mix in fertilizer and apply, addition is per kilogram soil 1g tyrosol, gather in the crops and within first 1 day, stop adding tyrosol, to allow tyrosol transform completely, romaine lettuce after results, measure Determination of Salidroside as stated above, its content (fresh weight) reaches as high as more than 0.15%.
Embodiment four, get feverfew Lettuce, carry out when growing 3 ~ 4 true leaves transplanting and (wherein add 100g tyrosol in per kilogram fertilizer in using sufficient base manure, mix with fertilizer) soil in, water permeable, conveniently planting type is cultivated, to results, in the Lettuce of results, rhodioside can be detected.
Embodiment five, get feverfew Lettuce, carry out when growing 3 ~ 4 true leaves transplanting and (wherein add 70g tyrosol in per kilogram fertilizer in using sufficient base manure, mix with fertilizer) soil in, water permeable, conveniently planting type is cultivated, to results, in the Lettuce of results, rhodioside can be detected.
Embodiment six, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 50g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), romaine lettuce after results, measures Determination of Salidroside as stated above, and its content (fresh weight) reaches as high as more than 0.05%.
Embodiment seven, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 20g tyrosol (can by tyrosol by water-soluble solution after add and bury, mixing), romaine lettuce after results, measures Determination of Salidroside as stated above, and its content (fresh weight) reaches as high as more than 0.02%.
Embodiment eight, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 80g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), the water (addition is that per kilogram soil adds 10g tyrosol) containing tyrosol is applied in 100 days backward soil, contain the water of tyrosol once (addition is that per kilogram soil adds 10g tyrosol) every applying in 10 days later, can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment nine, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 40g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), the water (addition is that per kilogram soil adds 10g tyrosol) containing tyrosol is applied in 50 days backward soil, contain the water of tyrosol once (addition is that per kilogram soil adds 5g tyrosol) every applying in 15 days later, after starting to bear fruit, add applying every 15 days and contain the water of tyrosol once (addition is that per kilogram soil adds 3g tyrosol), can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment ten, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by per kilogram in the soil of transplanting 10g tyrosol (can by tyrosol by water-soluble solution after add and bury, mixing), the water (addition is that per kilogram soil adds 5g tyrosol) containing tyrosol is applied in 70 days backward soil, contain the water of tyrosol once (addition is that per kilogram soil adds 5g tyrosol) every applying in 7 days later, after starting to bear fruit, add applying every 1 day and contain the water of tyrosol once (addition is that per kilogram soil adds 1g tyrosol), can pluck after fruit maturation, gather in the crops and within first 10 days, stop adding tyrosol.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 11, get plant of Solanaceae tomato, planting type is cultivated routinely, Post flowering, applies the water (addition is that per kilogram soil adds 10g tyrosol) containing tyrosol in soil, contain the water of tyrosol once every applying in 10 days later, can pluck after fruit maturation; Gather in the crops and within first 15 days, stop adding tyrosol.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 12, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by every strain romaine lettuce in the soil of transplanting 10g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), every 3 ~ 15 days, (initial stage was spaced apart 15 days, shorten interval time to 3 day gradually subsequently) apply containing the water of tyrosol in soil, also can mix in fertilizer and apply, addition is every strain 10g tyrosol, gather in the crops and within first 3 days, stop adding tyrosol, to allow tyrosol transform completely, romaine lettuce after results, measure Determination of Salidroside as stated above, its content (fresh weight) reaches as high as more than 0.15%.
Embodiment 13, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by every strain romaine lettuce in the soil of transplanting 0.1g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), in the soil of its growth, the water containing tyrosol was applied every 5 days after transplanting, tyrosol addition at every turn for previous 2 times (be namely respectively every strain romaine lettuce 0.2,0.4,0.8,1.6,3.2,6.4,12.8g, after reaching 12.8g, concentration no longer increases, and is 12.8g later at every turn).Gather in the crops and within first 5 days, stop adding tyrosol, to allow tyrosol transform completely, the romaine lettuce after results, measures Determination of Salidroside as stated above, and its content (fresh weight) reaches as high as more than 0.12%.
Embodiment 14, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by every strain romaine lettuce in the soil of transplanting 2g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), in soil, the water containing tyrosol is applied every 7 days, also can mix in fertilizer and apply, addition is every strain 1g tyrosol, gather in the crops and within first 1 day, stop adding tyrosol, to allow tyrosol transform completely, romaine lettuce after results, measure Determination of Salidroside as stated above, its content (fresh weight) reaches as high as more than 0.13%.
Embodiment 15, get feverfew Lettuce, carry out when growing 3 ~ 4 true leaves transplanting and (wherein add 100g tyrosol in every strain Lettuce fertilizer in using sufficient base manure, mix with fertilizer) soil in, water permeable, conveniently planting type is cultivated, to results, in the Lettuce of results, rhodioside can be detected.
Embodiment 16, get feverfew Lettuce, carry out when growing 3 ~ 4 true leaves transplanting and (wherein add 70g tyrosol in every strain Lettuce fertilizer in using sufficient base manure, mix with fertilizer) soil in, water permeable, conveniently planting type is cultivated, to results, in the Lettuce of results, rhodioside can be detected.
Embodiment 17, get feverfew romaine lettuce, conveniently planting type is cultivated, transplant when growing 5 ~ 6 true leaves, to add by every strain romaine lettuce in the soil of transplanting 20g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), romaine lettuce after results, can detect rhodioside.
Embodiment 18, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by every strain plant in the soil of transplanting 80g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), the water (addition is that every strain adds 10g tyrosol) containing tyrosol is applied in 100 days backward soil, contain the water of tyrosol once (addition is that every strain adds 10g tyrosol) every applying in 10 days later, can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 19, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by every strain in the soil of transplanting 40g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), the water (addition is that every strain adds 10g tyrosol) containing tyrosol is applied in 70 days backward soil, contain the water of tyrosol once (addition is that every strain earth adds 5g tyrosol) every applying in 10 days later, after starting to bear fruit, add applying every 5 days and contain the water of tyrosol once (addition is that every strain adds 3g tyrosol), can pluck after fruit maturation, gather in the crops and within first 8 days, stop adding tyrosol.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 20, get plant of Solanaceae tomato, planting type is cultivated routinely, after growing 4 ~ 6 true leaves, transplant when growing 4 ~ 6 true leaves, to add by every strain in the soil of transplanting 10g tyrosol (can by tyrosol by water-soluble solution after add in soil, mixing), the water (addition is that every strain soil adds 5g tyrosol) containing tyrosol is applied in 50 days backward soil, contain the water of tyrosol once (addition is that every strain adds 5g tyrosol) every applying in 15 days later, after starting to bear fruit, add applying every 1 day and contain the water of tyrosol once (addition is that every strain adds 1g tyrosol), can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 21, get plant of Solanaceae tomato, planting type is cultivated routinely, Post flowering, applies the water (addition is that every strain adds 10g tyrosol) containing tyrosol in soil, applied every 3 days later containing tyrosol water once, can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 22, get plant of Solanaceae tomato, after growing 4 ~ 6 true leaves, transplanting when growing 4 ~ 6 true leaves, transplanting in the soil using sufficient base manure (wherein add by every strain 1000g tyrosol, mix with fertilizer).Planting type is cultivated routinely, can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 23, get plant of Solanaceae tomato, after growing 4 ~ 6 true leaves, transplanting when growing 4 ~ 6 true leaves, transplanting in the soil using sufficient base manure (wherein add by every strain 700g tyrosol, mix with fertilizer).Planting type is cultivated routinely, can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 24, get plant of Solanaceae tomato, after growing 4 ~ 6 true leaves, transplanting when growing 4 ~ 6 true leaves, transplanting in the soil using sufficient base manure (wherein add by every strain 500g tyrosol, mix with fertilizer).Routinely planting type cultivate, can apply every day after bearing fruit containing tyrosol water once (addition is that every strain adds 0.1g tyrosol), can pluck after fruit maturation.Rhodioside can be detected in the root of tomato, stem, leaf and fruit.
Embodiment 25, get plant of Solanaceae tomato, after growing 4 ~ 6 true leaves, transplanting when growing 4 ~ 6 true leaves, transplanting in the soil using sufficient base manure (wherein add by every strain 100g tyrosol, mix with fertilizer).Routinely planting type cultivate, can apply every day after bearing fruit containing tyrosol water once (addition is that every strain adds 0.5g tyrosol), can pluck after fruit maturation.Rhodioside can be detected in the fruit of tomato.
Embodiment 26, chenopod spinach adopt usual manner cultivation, when applying fertilizer, in wherein adding tyrosol 50g by every strain, are cultured to results at every turn, and measure Determination of Salidroside, its content (fresh weight) reaches 0.05%.
Embodiment 27: get crucifer radish (i.e. radish), soil mode is cultivated routinely, when applying fertilizer, in wherein adding tyrosol 20g by every strain at every turn, can detect rhodioside after results in its leaf.
The invention also discloses the application of the above-mentioned plant planted, because containing rhodioside in plant, directly can use as health plant, also can be used as the extraction raw material of rhodioside, prepare health food, food additives or the medicine etc. for the preparation of tool rhodioside pharmacodynamic feature.
By the plant that above-described embodiment one to five obtains; can be used as health-care vegetable directly to eat, have that antifatigue, develop immunitypty, protection are cardiovascular, health care that anti anoxia, anti-ageing, neuroprotective cell, protection liver, the rhodioside such as anti-oxidant, antitumor, radioresistance have.
By the plant that above-described embodiment two and embodiment 12 obtain, when can be used as rhodioside extraction raw material, by the fresh plant of gathering, add water 5 times amount, decoct 1 hour, filter, it is 1.20 that filtrate is concentrated into relative density, let cool, with equal-volume extracting n-butyl alcohol 2 times, merge butanol extraction liquid, be evaporated to without alcohol taste, after adding 2 times of water gaging dilutions, with 1BV(bed volume)/h is by AB-8 large pore resin absorption column, first use the water washing of 2 times of bed volumes, discard, 10% ethanol washing of 2 times of bed volumes again, discard, use 4 times of bed volume 20% ethanol elutions again, collect eluent, be evaporated to dry, obtain crude extract, add after methyl alcohol dissolves in right amount, add the silica gel of 3 times of crude extract weight, mixing, add the silicagel column top of 20 times of crude extract weight, with 10 times of bed volume methanol-chloroform (2:8) solution, carry out wash-out, collect eluent, evaporated under reduced pressure, with absolute ethyl alcohol crystallization below 10 DEG C of 10 times of volumes, magma centrifugation, after absolute ethanol washing, vacuum drying, obtain the rhodioside that purity is greater than 99%.
By the plant that above-described embodiment ten obtains; as when preparing the raw material of health food; after the tamato fruit of 3kg maturation is scalded with boiling water; peeling, broken, squeeze the juice, obtain tomato juice; then in 1kg tomato juice, sodium chloride 20g and 0.5% sorbic acid is added; dissolve sterilizing after mixing, make health food, there is relieving sports fatigue, develop immunitypty, raising anoxia endurance, anti-oxidant and assistant protection function to hepatic injury.
When plant above-described embodiment two and embodiment 12 obtained is as food additives, add water the fresh plant of gathering 6 times amount, decoct 1 hour, decocting liquid filters, after filtrate is condensed into thick paste, get dry extract in 60 DEG C of vacuum dryings, namely can be used as food additives after pulverizing and use, such as add in beverage, beverage can be made to have the health care of rhodioside relieving fatigue.
The plant obtained by above-described embodiment two is as when preparing the medicine of tool rhodioside pharmacodynamic feature, can also can boiling 2 times by the romaine lettuce of results, add water 6 times amount at every turn, decoct 1 hour, decocting liquid merges filtration, after filtrate is condensed into thick paste, get dry extract in 60 DEG C of vacuum dryings, after pulverizing, get dried cream powder 350g, add medical starch 50g, pharmaceutical technology makes capsule or tablet routinely, is used for the treatment of and prevents altitude sickness, or being used for the treatment of coronary heart disease, cerebral infarction, the diseases such as senile dementia.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. containing a cultivation method for salidroside plant, it is characterized in that: in the solid dielectric of plant growth, add tyrosol, tyrosol addition is per kilogram solid dielectric 0.1 ~ 100g, or the tyrosol addition of every strain plant is 0.1 ~ 1000g; Described solid dielectric comprises soil, matrix or fertilizer.
2. a kind of cultivation method containing salidroside plant according to claim 1, it is characterized in that: at interval of 1 ~ 100 day, supplement and add tyrosol, tyrosol addition is per kilogram solid dielectric 0.1 ~ 100g, or the tyrosol addition of every strain plant is 0.1 ~ 1000g.
3. a kind of cultivation method containing salidroside plant according to claim 2, it is characterized in that: in the early stage of plant growth, at interval of 7 ~ 100 days, add tyrosol 1 time, in the later stage of plant growth, at interval of 1 ~ 15 day, add tyrosol 1 time, tyrosol addition is per kilogram solid dielectric 0.1 ~ 100g, or is every strain plant 0.1 ~ 1000g.
4. a kind of cultivation method containing salidroside plant according to Claims 2 or 3, is characterized in that: before plant harvest 1 ~ 15 day, stops adding tyrosol.
5. a kind of cultivation method containing salidroside plant according to any one of claims 1 to 3, is characterized in that: tyrosol addition is per kilogram solid dielectric 0.1 ~ 50g, or the tyrosol addition of every strain plant is 0.1 ~ 500g.
6. the application that the plant that a kind of cultivation method containing salidroside plant as described in any one of claim 1 to 5 goes out directly eats.
7. the plant that a kind of cultivation method containing salidroside plant as described in any one of claim 1 to 5 goes out extracts the application of raw material as rhodioside.
8. the plant that goes out of a kind of cultivation method containing salidroside plant as described in any one of claim 1 to 5 and extract thereof are for the preparation of the application of health food or food additives.
9. the plant that goes out of a kind of cultivation method containing salidroside plant as described in any one of claim 1 to 5 and extract thereof are for the preparation of the application with rhodioside pharmacodynamic feature medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310061826.4A CN103999648B (en) | 2013-02-27 | 2013-02-27 | A kind of cultivation method containing salidroside plant and plant application |
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| CN104212725B (en) * | 2014-09-15 | 2016-09-28 | 山西大学 | Improve rhodioside and the method for butyl alcohol content in Radix Rhodiolae tissue cultured seedling |
| CN104584900A (en) * | 2015-01-06 | 2015-05-06 | 湖南中医药大学 | Method for cultivating rhodiola rosea plants rich in rhodioloside and application |
| CN104542036A (en) * | 2015-01-06 | 2015-04-29 | 湖南中医药大学 | Cultivation method of vegetable containing salidroside and application |
| CN104584821B (en) * | 2015-01-06 | 2017-09-29 | 湖南中医药大学 | A kind of cultivation of plants method and application containing rhodioside |
| CN104585374A (en) * | 2015-01-06 | 2015-05-06 | 湖南中医药大学 | Preparation method and application of tea containing salidroside |
| CN104604503B (en) * | 2015-02-04 | 2017-01-04 | 浙江大学 | A kind of Biological control anti-root of Rhodiola sachalinensis maize ear rot and the method for growth-promoting |
| CN104620914B (en) * | 2015-02-04 | 2016-09-14 | 浙江大学 | A kind of biological control method of the disease-resistant growth-promoting of Radix Apioris Fortunei (Radix Lespedezae Buergeri) |
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