CN104585196A - Tetramycin compound and application thereof - Google Patents
Tetramycin compound and application thereof Download PDFInfo
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- CN104585196A CN104585196A CN201510059405.7A CN201510059405A CN104585196A CN 104585196 A CN104585196 A CN 104585196A CN 201510059405 A CN201510059405 A CN 201510059405A CN 104585196 A CN104585196 A CN 104585196A
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Abstract
The invention relates to the field of research and development of biological pesticide preparations, and particularly relates to a tetramycin compound and application thereof, which are a divisional application of an invention named 'tetramycin compound and application' having an application number of 2013106143470 and being applied on November 26, 2013. The tetramycin compound comprises tetramycin and an active substance, wherein the active substance belongs to pyrimidines, including one or more of pyrimidyl methanols, 2-amino-pyrimidyls, phenylaminopyrimidines. Compared with the prior art, the tetramycin compound and application thereof have the advantages that the risk of resistant fungal strain selectivity can be reduced, the service life of the active substance can be prolonged, the prevention and treatment range can be enlarged, the disease prevention effect can be improved, the drug resistance of pathogenic bacteria can be retarded or overcome, the cost can be reduced, and the number of pesticide application times can be reduced.
Description
The divisional application that the present patent application is application number is 2013106143470, the applying date is on November 26th, 2013, denomination of invention is " tetramycin complex and application ".
Technical field
The present invention relates to biopesticide preparaton research and development field, in particular to tetramycin complex and application.
Background technology
At present, the pesticide species for plant protection is various, and mostly pesticide species is meddlesome, but also brings a lot of problem.Mostly agricultural chemicals is that a worm one is controlled, a medicine one is controlled, thus agricultural chemicals can only be prevented and treated for a kind of insect pest or disease, does not produce effect, even cause the accident of the underproduction to other murder by poisoning or disease, and replay in field, No kernels or seeds are gathered, as in a year of scarcity for even ruining of having.The plant protection technology that a this worm one is controlled, a medicine one is controlled is not suitable with the needs of agricultural development, and peasant controls in the urgent need to a medicine more, sick worm is doublely controlled, economize medicine saving of labor, protect the dispenser new technology of producing volume increase, easy to use, remarkable benefit.
Summary of the invention
The object of the present invention is to provide tetramycin complex and application, to solve the above problems.
Embodiments providing a kind of tetramycin complex, comprising: tetramycin and active substance; Described active substance is miazines, described miazines comprise following one or more: pyrimidinyl carbinoles class, 2-amino-pyhmidines, aniline pyrimidine class.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
The tetramycin complex that the embodiment of the present invention provides and application are compared with prior art, tetramycin possesses the effect of sterilization, control germ, disease, tetramycin and active substance composite after, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Embodiment
Be described in further detail the present invention below by specific embodiment, content mentioned in embodiment is not limitation of the invention, and material prescription is selected to suit measures to local conditions and on result without substantial impact.In these embodiments, unless otherwise indicated, all percentage is all weight percentage.
Tetramycin complex comprises: tetramycin and active substance; Described active substance is miazines, described miazines comprise following one or more: pyrimidinyl carbinoles class, 2-amino-pyhmidines, aniline pyrimidine class.
The embodiment of the present invention additionally provides a kind of application of tetramycin complex, for controlling plant diseases, insect pest and weeds, and/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
Biopesticide tetramycin is a kind of high-effective broad-spectrum fungicide; to the plurality of plant diseases on the crops such as cereal, fruit tree, vegetables, all there is protection and therapeutic activity, especially to fungal diseases such as gray mold crops such as () tomato, cucumber, capsicums, powdery mildew (wheat class, melon, fruit tree etc.), scabs (apple, pears etc.), there is good control efficiency.But this series bactericidal agent action site is single, and germ easily produces adaptive variation to medicament, the preventive effect of medicament is reduced.Tetramycin and active substance composite after can play synergistic function, by by having the active substance of the different mechanism of action and its combination, reduce refractory fungal bacterial strain optionally dangerous, service life of extending active component, add the fungicidal spectrum of single component, expand prevention and treatment range; Improve protection effect; Can delay or overcome the pesticide resistance of germ; Both saved the application times that cost again reduces agricultural chemicals, active response country advocates environmental protection agricultural chemicals.
Next, the present invention will describe this tetramycin complex in detail by some specific embodiments, and the embody rule situation of tetramycin complex:
Tetramycin complex, comprising: tetramycin and active substance; Described active substance is miazines, described miazines comprise following one or more: pyrimidinyl carbinoles class, 2-amino-pyhmidines, aniline pyrimidine class.
In certain embodiments, when active substance comprises pyrimidinyl carbinoles class, pyrimidinyl carbinoles class comprise following one or more: ancymidol, fenarimol or nuarimol;
In certain embodiments, when active substance comprises 2-amino-pyhmidines, 2-amino-pyhmidines comprise following one or more: the spirit of sulphur phonetic bacterium, Milcurb or Milstem;
In certain embodiments, when active substance comprises aniline pyrimidine class, aniline pyrimidine class comprise following one or more: ring third pyrimidine, pyrimethanil or mepanipyrim;
In certain embodiments, when active substance also comprise following one or more other there is the compound of different structure: diazosulfide, irritated pathogenicity proteins, anilazine, blasticidin-S, chinomethionat, chloroneb, tpn, cymoxanil, dichlone, diclomezin, botran, the mould prestige of second, dimethomorph, Delan, Grandox fumigant, famoxadone, Fenamidone, fentin, ferimzone, fluazinam, flusulfamide, microbial inoculum is phonetic, aliette, dislike mould spirit, kasugarnycin, methasulfocarb, Pencycuron, rice blast peptide, polyoxin, probenazole, Propamocarb, pyroquilon, quinoxyfen, pcnb, Cosan, triazoxide, tricyclazole, triforine, valida, Propineb,
Wherein the mass concentration of tetramycin in tetramycin complex can adjust arbitrarily in 0.15-99%, according to the type of compound and required effect, reaches better balanced toxicity and residual by adjustment mass concentration (or the concentration that weighs).
The rate of application of inventive mixture is 0.6-400 gram/ha.
Such as: in certain embodiments, the mass concentration of tetramycin is 0.15%, and concentration is lower.
In certain embodiments, the mass concentration of tetramycin is 15%, or 35%, or 50%, or 70%.
In certain embodiments, the mass concentration of tetramycin is 99%, and concentration is higher, in tetramycin complex, play Main Function.
The finished product of this tetramycin complex can make in various forms one or more, such as: aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent (ULV).Be made into various forms and facilitate the storage of tetramycin complex, transport and follow-up use.
Such as:
A) aqua
The composite mixture of 10 weight portion is dissolved in 90 weight parts waters or water-soluble solvent.Or add wetting agent or other auxiliary agent, composite mixture dissolves through water dilution, obtains the aqua that tetramycin complex mass percentage content is 10% in this way.
B) dispersible agent
Be dissolved in 70 weight portion cyclohexanone by the composite mixture of 20 weight portion, and add 10 parts per weight dispersing agent as PVP(polyvinyl pyrrolidone), dilute with water obtains dispersion, obtains the dispersible agent that tetramycin complex mass percentage content is 20% in this way.
C) missible oil
The composite mixture of 15 weight portion to be dissolved in 75 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case), dilute with water obtains emulsion, obtains the missible oil that tetramycin complex mass percentage content is 15% in this way.
D) microemulsion
The composite mixture of 10 weight portion to be dissolved in 20 weight portion cyclohexanones and to add calcium dodecyl benzene sulfonate (being 20 weight portions in each case), dilute with water obtains microemulsion, obtains the microemulsion that tetramycin complex mass percentage content is 5% in this way.
E) emulsion
The composite mixture of 25 weight portion to be dissolved in 35 parts by weight of xylene and to add calcium dodecyl benzene sulfonate and castor oil ethoxylate (being 5 weight portions in each case).This mixture to be introduced in 30 weight parts waters by emulsifier (Ul traturrax) and make equal phase emulsion.Dilute with water obtains emulsion, obtains the emulsion that tetramycin complex mass percentage content is 25% in this way.
F) suspension
In the ball mill stirred, the composite mixture of 20 weight portion is pulverized and adds 10 parts per weight dispersing agent and wetting agent and 70 weight parts waters or organic solvent, obtain reactive compound suspension in small, broken bits, dilute with water obtains stable reactive compound suspension, obtains the suspension that tetramycin complex mass percentage content is 20% in this way.
G) gel formulation
In ball mill, the composite mixture of 20 weight portion, 10 parts per weight dispersing agent, 1 weight portion gelling agent and 70 weight parts waters or organic solvent grinding are obtained delicate suspensions, dilute with water is obtained the stabilized with mixture suspension that tetramycin complex mass percentage content is 20% in this way.
H) ultra low volume solvent
The composite mixture of 10 weight portion is dissolved in 90 weight parts organic solvent as in dimethylbenzene, obtains the product used without dilution that the composite mixture quality degree of tetramycin is 10%.
This tetramycin complex can be used for the mode controlling disease similar to other preparations, insect pest and weeds etc.Simultaneously compared with unitary agent, can controlling disease, insect pest and weeds better, play the effect of Synergistic.Both tetramycin complex can be used and also tetramycin and active substance can be used successively.
In the application of this tetramycin complex, may be used for for controlling plant diseases, insect pest and weeds, also for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
This tetramycin complex can be used as disease on the plant in control different season and plant part (fruit, flower, leaf, stem, stem tuber, root), insect pest, weeds, and also protective plant late growing stage part provides growth institute energy requirement from germ infringement and for plant.
The application target crop of the tetramycin complex of the present invention's protection generally includes but is not limited to following plants kind: cereal (wheat, barley, rye, oat, paddy rice, corn, Chinese sorghum and related species); Beet (sugar beet and fodder beet); The operatic circle, drupe and berry (kiwi fruit, strawberry, raspberry, gooseberry, fruit mulberry, fig, pomegranate, carambola, guava etc.); Leguminous plant (Kidney bean, little inclined beans, pea, soybean, red bean); Oil plants (rape, shepherd's purse, opium poppy, olive, sunflower, coconut, castor oil plant, cocoa bean, peanut); Mellon plant (pumpkin, cucumber, muskmelon, balsam pear, wax gourd, watermelon); Fibre plant (cotton, flax, hemp, jute); Citrus fruit (orange, lemon, glucose shaddock, bar tangerine); Vegetables (spinach, asparagus lettuce, asparagus, wild cabbage, carrot, onion, tomato, potato, red pepper); Lauraceae (avocado, Chinese cassia tree, camphor) or such as tobacco, nut, coffee, eggplant, sugarcane, tea, pepper, comprise grape, banana, the vining plant of grass roots and natural rubber plant and ornamental plants (flowers, shrub, broad-leaved tree and evergreen plant) etc.It should be noted that, this inventory does not represent any restriction.
Tetramycin complex of the present invention also can be used for control industrial materials fungal infection (especially by mould microbial), bacterium or insect pest, and safeguard industries material infects from fungal attack with at this fungal infection rear reduction occurring or eradicates industrial materials.Industrial materials include but not limited to organic and inorganic material timber, paper, leather, natural or synthetic fibers and composite, such as particle board, plywood, wallboard etc.The present invention can be applied to this material with the consumption effectively suppressed.
Composite mixture of the present invention is used for the Prevention and Curation that disease, worm, crop smothering occur in plant, seed, soil, region, material and spatial dimension, simultaneously for plant provides growth institute energy requirement.
In use, its application form is this tetramycin complex: any one in preparaton, spray solution, emulsion, paste, oil.Type of service depends on and is specifically intended to object; Under certain conditions, should guarantee that tetramycin complex is meticulous and distribute equably.
In certain embodiments, when the application form of tetramycin complex is preparaton, tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
In certain embodiments, when tetramycin complex and the blended rear use of solvent, solvent comprise following one or more: water, arsol (as toluene, dimethylbenzene), paraffin (as mineral oil fractions), alcohols (as methyl alcohol, butanols, amylalcohol, benzylalcohol), ketone (as cyclohexanone, gamma-butyrolacton), pyrrolidones (NMP, NOP), acetic acid esters (ethylene acetate), dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester.
In certain embodiments, when tetramycin complex and the blended rear use of emulsifier, emulsifier comprise following one or more: nonionic and anion emulsifier (as polyoxyethylene aliphatic alcohol ether, alkylsulfonate and arylsulphonate);
In certain embodiments, when tetramycin complex and the blended rear use of surfactant, surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
When tetramycin complex acts on plant seed, the mode of preparaton can be made into, except above-mentioned each blending constituent, can also mix with adhesive and/or gelling agent and/or colouring agent.
When the application form of tetramycin complex is spray solution, emulsion, paste, oil, the material of dispersion is that mid-boiling point arrives high boiling mineral oil fractions, as kerosene or diesel oil, in addition the oil of coal tar and plant or animal origin is also had, aliphatic series, ring-type and aromatic hydrocarbon, such as toluene, dimethylbenzene, paraffin, tetrahydronaphthalene, alkylated naphthalene or derivatives thereof, methyl alcohol, ethanol, propyl alcohol, butanols, cyclohexanol, cyclohexanone, isophorone, intensive polar solvent, such as dimethyl sulfoxide (DMSO), 1-METHYLPYRROLIDONE and water.
Tetramycin complex adopts moisture type of service to make emulsion concentrates, paste by adding water in the application.For preparation emulsion, paste or oil dispersion, can by wetting agent, thickener, dispersant or emulsifier by this material directly or to be dissolved in after in oil or solvent homogenizing in water.But, the concentrate that is made up of composite mixture, wetting agent, thickener, dispersant or emulsifier and suitable solvent or oil can also be prepared and this concentrate is suitable for dilute with water.
In certain embodiments, tetramycin complex can be used for controlling disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
When tetramycin complex is for preventing and treating fungal disease, fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When tetramycin complex is for preventing and treating bacterial disease, bacterial disease comprise following one or more: pseudomonas, bacillus;
When tetramycin complex is used for preventing and treating virus disease, virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When tetramycin complex be used for pest control time, insect pest comprise following one or more: insect, mite class, snail, muroid;
When tetramycin complex be used for controlling weeds time, weeds comprise following one or more: grass, cyperus, broad-leaf weeds;
Provide the specific embodiment of several tetramycin complex below, should understand, these embodiments are only for further illustrating of the present invention, and be not used in and limit the scope of the invention, without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Example one tetramycin and ancymidol composite
Wherein, active material component selects ancymidol.The formula of the tetramycin complex of preparation: 6% tetramycin, 12% ancymidol, 4% agriculture breast 500# (calcium dodecyl benzene sulfonate), 3%Span-60# (sorbitan monostearate), 3% agriculture breast 2201#, 2% triphenyl phosphite, dimethylbenzene mends 100%.Fully dissolve after above-mentioned raw materials mixing, the obtained 18% tetramycin ancymidol missible oil of mixing.Wherein tetramycin is reagent agent A, and ancymidol is reagent agent B.
Method of testing:
Take appropriate reagent agent acetone solution, with water, each reagent agent is mixed with the solution (in solution, content of acetone is not more than 10%) of described concentration.The emulsifier Tween 80 of 0.1% (volume) is added in this solution, by described proportioning, each reagent agent equal proportion is mixed, obtain described proportioning and concentration.
Percentage evaluation is infected by measuring leaf area.These percentages are changed into effect.Use Abbot formulae discovery effect (W), namely obtain the effect observed:
W=(1-α/β) × 100, in formula: α: the fungal infection percentage of process plant; β: the fungal infection percentage of untreated (blank) plant; For " 0 ", effect represents that the level that infects of process plant is identical with the level that infects of untreated control plant; For " 100 ", effect represents that process plant is not infected.
The expection effect of composition uses Colby formula determine and compare with the effect observed:
E=x+y-xy/100; In formula: E: the expection effect (effect of the calculating in following table) when working concentration is the composition of the reagent agent A of a and b and B, represents with the % of untreated control; X: effect when working concentration is the reagent agent A of a, represents with the % of untreated control; Y: effect when working concentration is the reagent agent B of b, represents with the % of untreated control.
The test of Application Example control wheat powdery mildew, conservation treatment.
Be that the potted plant wheat seedling leaf reagent agent concentration of " the Liao Dynasty spring No. 10 " dilution as described below is sprayed to drip point by cultivar.After 24 hours, be inoculated in test plants with the spore dilution of Powdery Mildew.Test plants being placed in 22 ~ 24 DEG C with relative atmospheric humidity is that 75% climatic chamber regulates 7 days.Then naked eyes measure the development degree of infection process on leaf.The activity of each independent medicament and tetramycin complex control wheat powdery mildew. data result is in table 1 and table 2.
Table 1
Table 2
Test shows that 18% tetramycin ancymidol missible oil has good control efficiency to wheat powdery mildew, is better than corresponding single dose.Composite single dose is more best than diseases prevention Controlling effect with the quality of 4:1.
Example two tetramycin and Milcurb composite
Wherein, active material component selects Milcurb.The formula of the tetramycin complex of preparation: 7% tetramycin, 9% Milcurb, 6% emulsifier such as agriculture breast 500# (calcium dodecyl benzene sulfonate), 2% agriculture breast 2201#, 3% agriculture breast 500#, 2% methyl alcohol, 5% dimethylbenzene, 1.2% epoxychloropropane, 0.4% aceticanhydride, deionized water are mended to 100%, above-mentioned raw materials are fully dissolved the obtained 16% tetramycin Milcurb microemulsion of mixing.Wherein tetramycin is reagent agent A, and Milcurb is reagent agent B.
Application Example is to the persistence of the late blight of tomato that phytophthora infestans causes
Solvent/emulsifier volume ratio is used to be that reactive compound is made and comprised 25mg reactive compound and the stock solution being made into 10ml by the acetone of 99: 1 and/or the mixture of DMSO and emulsifier EL (having the wetting agent based on ethoxylated alkylphenol of emulsification and peptizaiton) alone or in combination.Then this mixture water is made into 100ml.This stock solution is diluted to following activity compound concentration with described solvent/emulsifier/aqueous mixtures.
Be that the leaf activity compound concentration of the pot plant of rosy clouds powder aqueous suspension as described below is sprayed to drip point by cultivar.By the moisture spore suspension inoculation of leaf phytophthora infestans after 5 days.Then plant being placed on temperature is in the steam-laden room of 18-20 DEG C.After 6 days, untreated but late blight on the check plant infected develops into and can measure the degree infecting % by naked eyes.
Determination data is as table 3:
The leaf area percentage that infects that naked eyes measure is changed into the effect represented relative to the % of untreated control.
Use Abbot formula by following radix effect (E):
E＝(1-α/β)·100
α corresponds to the fungal infection percentage of process plant, and
β corresponds to the fungal infection percentage of untreated (contrast) plant.
Effect is that infecting of 0 expression process plant is on close level in untreated check plant; Effect is that 100 expression process plants are not infected.
Result of the test shows, owing to coordinating synergistic effect, inventive mixture significantly has more activity compared with use Colby formula predictions.
Example three tetramycin and ring third pyrimidine composite
Wherein, active material component selects ring third pyrimidine.
The technical solution used in the present invention: a kind of tetramycin ring third pyrimidine emulsifiable concentrate, comprise the component of following mass percent: tetramycin 1-3%, ring third pyrimidine 4-12%, octyl phenol polyglycol ether (4-5 moles of ethylene oxide) 3%, dodecyl benzoic acid calcium 3%, castor oil polyglycol ether (35 moles of ethylene oxide) 4%, cyclohexanone 30%, xylene mixture 50%.Wherein tetramycin is reagent agent A, and ring third pyrimidine is reagent agent B.
This mixture of dilute with water can obtain the diluting emulsion of any concentration used in plant protection.
The effect of control wheat leaf rust
Medicine liquid spray process vegetative period of preparing with the preparation containing single A, B or tetramycin complex is respectively that the wheat plant of 7 days is until there is drop to drip from plant.Process plant is infected with fungal spore suspension after 48 hours.Cultivate at relative moisture 90-100% and temperature 20 DEG C and within 2 days, to be placed in the climatic cabinate of 21 DEG C 9 days.With fungal infection 11 days post-evaluation Fungus infestation.
When the effect of tetramycin complex is greater than each single reagent agent effect sum, then there is synergistic effect.
Colby formula, computational methods are as follows
Result is as follows: E=x+y-xy/100;
In formula: E: the expection effect (effect of the calculating in following table) when working concentration is the composition of the reagent agent A of a and b and B, represents with the % of untreated control; X: effect when working concentration is the reagent agent A of a, represents with the % of untreated control; Y: effect when working concentration is the reagent agent B of b, represents with the % of untreated control.
Table 4: tetramycin complex is to the control efficiency of wheat leaf rust
Can find out as above data and tetramycin ring third pyrimidine suspending agent two active principle ratio account for 22 parts of B at A to account for the effect of the proportioning control wheat leaf rust in the of 6 parts maximum, synergistic effect is the most obvious, is addition.
Example four tetramycin and pyrimethanil composite
Wherein, active material component selects pyrimethanil.Wherein tetramycin is reagent agent A, and pyrimethanil is reagent agent B
Tetramycin and pyrimethanil different ratio co-toxicity are tested
The formula of water-soluble preparaton: the mixture of 10 weight portion A, B or A and B is dissolved in 90 weight parts waters or water-soluble solvent.As selection, add wetting agent or other auxiliary agents.Reactive compound dissolves through water dilution.Obtain that there is the preparation that reactive compound weight percent content is 10% like this.
Reagent agent
0.3% tetramycin, above-mentioned former medicine provides by Liaoning biological Co., Ltd of micro-section laboratory;
The former medicine of 97% pyrimethanil, commercial.
Tetramycin compound drug
Test target
Never executed tetramycin and pyrimethanil and cucumber of the same type to gather potted plantly be separated, for examination cucumber variety: Chang Chun Mi Ci from Heilungkiang.
Test method
First use the former medicine of acetone solution, appropriate two former medicines are made into 5 different ratio by the result according to preliminary experiment, each process is diluted to respectively 5 concentration gradients with acetone more stand-by, 5 0.3% tetramycins are respectively 12:28 with the ratio of pyrimethanil different ratio active ingredient; 14:26; 16:24; 18:22; 20:20.
By susceptible cucumber variety Chang Chun Mi Ci cultivation in pot for growing seedlings, be placed in greenhouse and cultivate, plant grows to three to four leaf after dates, gathers the blade of identical leaf age, for cultivation and the mensuration of cucumber powdery mildew's pathogen.
The cultivation of cucumber powdery mildew's pathogen and the preparation of spore suspension
Cucumber powdery mildew's pathogen adopt plants method 20 DEG C, cultivate under the condition of 12h alternation of light and darkness, every 30d turns culture 1 time.With the conidium on sterile water wash-out incidence of leaf during inoculation, being mixed with spore concentration is l × 10
6the suspension of individual/mL.
Mixture toxicity test and interpretation of result
Carry out the toxicity test of mixture according to mixed ratio by single dose toxicity test method.
Table 5 tetramycin and pyrimethanil are to the toxicity test of powdery mildew of cucumber
Field trial prevents and treats powdery mildew of cucumber, gray mold
Test method
Test on Chaoyang City, Liaoning Province cucumber ground, 4 points are investigated in every community, and often some investigation 2 strains, record occurring degree by staging,
Grade scale is as follows:
0 grade: anosis;
1 grade: sorus area accounts for less than 5% of whole leaf area;
3 grades: sorus area accounts for below the 6%-10% of whole leaf area;
5 grades: sorus area accounts for below the 11%-20% of whole leaf area;
7 grades: sorus area accounts for below the 21%-50% of whole leaf area;
9 grades: sorus area accounts for more than 50% of whole leaf area;
Control time and number of times
Test is investigated 4 times altogether, and before medicine, radix investigation, investigates after 3 days, 7 days and medicine after medicine for 15 days.
Drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(1-(before blank district medicine after disease index × treatment region medicine disease index)/(after blank district medicine before disease index × treatment region medicine disease index)) × 100.
Whether after the dispenser of poisoning investigation method, continuous 15d estimates medicament has poisoning to crop.
Result of the test and analyze the effect that each process prevents and treats powdery mildew of cucumber.(table 6)
The effect of powdery mildew of cucumber prevented and treated by table 6 respectively process
The composite bactericide of 40% tetramycin pyrimethanil SC (16:24) controls successful higher than tetramycin and pyrimethanil single dose for powdery mildew of cucumber as shown in Table 6, and after medicine, the bactericidal effect of 15 days is respectively 91.12, and 92.43,93.54.Bactericidal effect increases progressively with the increase of dosage.According to field range estimation, within the scope of test dose, plant growth is normal, and the poisoning phenomenon to cucumber does not all appear in each treatment agent, illustrates that it is safe to cucumber.Advise that the bactericide different from the mechanism of action is used in combination to delay the drug-fast generation of germ.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a tetramycin complex, is characterized in that, comprising: tetramycin and active substance; Described active substance is miazines, described miazines comprise following one or more: pyrimidinyl carbinoles class, 2-amino-pyhmidines, aniline pyrimidine class.
2. tetramycin complex according to claim 1, is characterized in that, the mass concentration of described tetramycin in described tetramycin complex is 0.15-99%.
3. tetramycin complex according to claim 1, is characterized in that, described tetramycin complex is any one or more in aqua, dispersible agent, missible oil, microemulsion, emulsion, suspension, gel formulation, ultra low volume solvent.
4. the tetramycin complex according to any one of claim 1-3, is characterized in that, when described active substance comprises pyrimidinyl carbinoles class, described pyrimidinyl carbinoles class comprise following one or more: ancymidol, fenarimol or nuarimol;
When described active substance comprises 2-amino-pyhmidines, described 2-amino-pyhmidines comprise following one or more: the spirit of sulphur phonetic bacterium, Milcurb or Milstem;
When described active substance comprises aniline pyrimidine class, described aniline pyrimidine class comprise following one or more: ring third pyrimidine, pyrimethanil or mepanipyrim.
5. the application of the tetramycin complex described in any one of claim 1-4, is characterized in that, for controlling plant diseases, insect pest, weeds; And/or for preventing and treating fungi in timber, paper, leather, fiber, composite, bacterium, insect pest.
6. the application of tetramycin complex according to claim 5, is characterized in that, the application form of described tetramycin complex is: any one in preparaton, spray solution, emulsion, paste, oil.
7. the application of tetramycin complex according to claim 6, it is characterized in that, when the application form of described tetramycin complex is preparaton, described tetramycin complex and solvent and/or carrier and/or emulsifier and/or the blended rear use of surfactant.
8. the application of tetramycin complex according to claim 7, is characterized in that,
When described tetramycin complex and the blended rear use of solvent, described solvent comprise following one or more: water, arsol, paraffin, alcohols, ketone, pyrrolidones, acetic acid esters, dihydroxylic alcohols, fatty acid dimethylamides, fatty acid and fatty acid ester;
When described tetramycin complex and the blended rear use of carrier, described carrier comprise following one or more: the kaolin ground, the clay ground, the talcum ground, the chalk ground, the silica ground, the silicate ground;
When described tetramycin complex and the blended rear use of emulsifier, described emulsifier comprise following one or more: nonionic and anion emulsifier;
When described tetramycin complex and the blended rear use of surfactant, described surfactant comprise following one or more: lignosulphonic acid, naphthalene sulfonic acids, phenolsulfonic acid, the alkali metal salt of dibutyl naphthalenesulfonic acid, alkali salt, ammonium salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, aliphatic alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, the condensation product of naphthalene or naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, alkyl phenyl polyglycol ether, tributyl phenyl polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, alcohol and fatty alcohol/ethylene oxide condensation product, ethoxylated castor oil, polyoxyethylene alkyl ether, ethoxylation polyoxypropylene, laruyl alcohol polyglycol ether acetal, sorbitol ester, lignin sulfite waste liquor, methylcellulose.
9. the application of tetramycin complex according to claim 5, is characterized in that, for described disease comprise following one or more: fungal disease, bacterial disease, virus disease, nematodosis.
10. the application of tetramycin complex according to claim 9, is characterized in that,
When described tetramycin complex is for preventing and treating fungal disease, described fungal disease comprise following one or more: Deuteromycotina fungi, Basidiomycotina fungi, Ascomycotina fungi, Zygomycotina fungi, Mastigomycotina fungi;
When described tetramycin complex is for preventing and treating bacterial disease, described bacterial disease comprise following one or more: pseudomonas, bacillus;
When described tetramycin complex is used for preventing and treating virus disease, described virus disease comprise following one or more: double-stranded DNA virus, single-stranded DNA viruses, diplornavirus, single strand RNA virus;
When described tetramycin complex be used for pest control time, described insect pest comprise following one or more: insect, mite class, snail, muroid;
When described tetramycin complex be used for controlling weeds time, described weeds comprise following one or more: grass, cyperus, broad-leaf weeds.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696652A (en) * | 2012-06-29 | 2012-10-03 | 青岛星牌作物科学有限公司 | Sterilizing composite for controlling pyricularia oryzae |
| CN103300024A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Sterilization composition containing tetramycin |
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