CN104548204A - Method for preparing fat granular filler - Google Patents
Method for preparing fat granular filler Download PDFInfo
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- CN104548204A CN104548204A CN201310508120.8A CN201310508120A CN104548204A CN 104548204 A CN104548204 A CN 104548204A CN 201310508120 A CN201310508120 A CN 201310508120A CN 104548204 A CN104548204 A CN 104548204A
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- fat
- lipochondrion
- centrifuge
- rotating speed
- minutes
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Abstract
The invention provides a method for preparing fat granular filler. The method comprises the following steps: (1) equally dividing a proper amount of fat into 2 parts; (2) separating and extracting one part of the fat in a centrifugal machine at a rotating speed of 3000r/min for 5 minutes to obtain fat granules; (3) putting the separated fat granules in brine ice to store for later use; (4) extracting the preadipocyte of the other part of fat; and (5) uniformly mixing and stirring the extracted preadipocyte and the centrifuged fat granules to obtain the fat granular filler. The compound fat granular filler is completely obtained from a human body, and is safe and reliable.
Description
Technical field
The present invention relates to medical science fat transplantation technical field, particularly relate to a kind of preparation method of lipochondrion implant.
Background technology
Usually be all that firm silicone rubber or other allosome materials are filled into a certain tissue of health as implant in traditional grand chin, the grand operation such as to enlarge the bosom, and this implant can produce repulsion after being placed in human body for a long time, dangerous.
Summary of the invention
The object of the invention is to the defect solving the existence of above-mentioned prior art, a kind of lipochondrion implant preparation method that is safe and reliable, property once and for all is provided.
A preparation method for lipochondrion implant, comprises the following steps:
1), get appropriate fat and be divided into 2 equal portions;
2), wherein an equal portions fat put into centrifuge and carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 3000r/min, 5 minutes time;
3), isolated lipochondrion is put into brine ice to save backup;
4) cell before the fat of another equal portions fat, is extracted;
5), by extract fat before cell and centrifugal after lipochondrion mixing and stirring, lipochondrion implant of the present invention can be obtained.
Further, the fatty precellular method of described extraction comprises the following steps:
1), another equal portions fat is put into centrifuge carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 1000r/min, 3 minutes time;
2), by the lipochondrion of extraction put into constant temperature oscillation case, add after collagen protein does not have enzyme and vibrate, vibration temperature is 27 DEG C, and the time is 40 minutes, and rotating speed is 220r/min;
3), add appropriate brine ice, proceed vibration, vibration temperature is 27 DEG C, and the time is 3 minutes, and rotating speed is 1000r/min;
4), by the lipochondrion after vibration put into centrifuge and be separated, wherein, the rotating speed of centrifuge is 1200r/min, 8 minutes time, repeatedly 3 times centrifugal, cell before described fat can be obtained.
Further, described brine ice is the normal saline taken out from refrigerator.
Further, described centrifugalize has adopted totally enclosed single tank mode to be separated.
Composite fat granule implant of the present invention all takes from human body, safe and reliable.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly, be clearly and completely described below to the technical scheme in the present invention, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment:
Prepare the method for composite fat granule implant:
1), from the abundant portion of own fat (as waist, abdominal part etc.) extract fat appropriate, and be divided into 2 equal portions;
2), wherein an equal portions fat put into centrifuge and carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 3000r/min, 5 minutes time;
3), isolated lipochondrion is put into brine ice to save backup;
4), another equal portions fat is put into centrifuge carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 1000r/min, 3 minutes time;
5), by the lipochondrion of extraction put into constant temperature oscillation case, add after collagen protein does not have enzyme and vibrate, vibration temperature is 27 DEG C, and the time is 40 minutes, and rotating speed is 220r/min;
6), add appropriate brine ice, proceed vibration, vibration temperature is 27 DEG C, and the time is 3 minutes, and rotating speed is 1000r/min;
7), by the lipochondrion after vibration put into centrifuge and be separated, wherein, the rotating speed of centrifuge is 1200r/min, 8 minutes time, repeatedly 3 times centrifugal, cell before fat can be obtained;
8), by extract fat before cell and centrifugal after lipochondrion mixing and stirring, lipochondrion implant of the present invention can be obtained.
Wherein, described brine ice is the normal saline taken out from refrigerator; Described centrifugalize has adopted totally enclosed single tank mode to be separated.
Composite fat granule implant implant of the present invention has following technical advantage:
1. be taken at autologous, paramount safety
Implant all derives from autologous, and safety is far superior to any allosome material.
2. survival rate is high, easy to operate
Fat granules, can not produce repulsion, can combine with autogenous cell moment.
3. stimulate regeneration, physical alterations
Autologous dryness fat can produce the effect stimulating regeneration to transplantation site.Physical alterations.
4. autologous bound drug, puts things right once and for all.
Anti-infection ability is strong, can in the short time with body cell perfect adaptation, general autoinjector art, can absorb by human body, often through multiple injection, just can be effective! The high-quality extract of the soft Technology for Gold of autologous fat through autologous fat and the perfect combination of pharmaceutical preparation, can reach and put things right once and for all.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (4)
1. a preparation method for lipochondrion implant, is characterized in that, comprises the following steps:
1), get appropriate fat and be divided into 2 equal portions;
2), wherein an equal portions fat put into centrifuge and carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 3000r/min, 5 minutes time;
3), isolated lipochondrion is put into brine ice to save backup;
4) cell before the fat of another equal portions fat, is extracted;
5), by extract fat before cell and centrifugal after lipochondrion mixing and stirring, lipochondrion implant of the present invention can be obtained.
2. the preparation method of lipochondrion implant according to claim 1, is characterized in that, the fatty precellular method of described extraction comprises the following steps:
1), another equal portions fat is put into centrifuge carry out separation and Extraction lipochondrion, wherein, the rotating speed of centrifuge is 1000r/min, 3 minutes time;
2), by the lipochondrion of extraction put into constant temperature oscillation case, add after collagen protein does not have enzyme and vibrate, vibration temperature is 27 DEG C, and the time is 40 minutes, and rotating speed is 220r/min;
3), add appropriate brine ice, proceed vibration, vibration temperature is 27 DEG C, and the time is 3 minutes, and rotating speed is 1000r/min;
4), by the lipochondrion after vibration put into centrifuge and be separated, wherein, the rotating speed of centrifuge is 1200r/min, 8 minutes time, repeatedly 3 times centrifugal, cell before described fat can be obtained.
3. will go the method described in 1 according to right, it is characterized in that, described brine ice is the normal saline taken out from refrigerator.
4. will go the method described in 1 according to right, it is characterized in that, described centrifugalize has adopted totally enclosed single tank mode to be separated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310508120.8A CN104548204A (en) | 2013-10-25 | 2013-10-25 | Method for preparing fat granular filler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310508120.8A CN104548204A (en) | 2013-10-25 | 2013-10-25 | Method for preparing fat granular filler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104548204A true CN104548204A (en) | 2015-04-29 |
Family
ID=53065966
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310508120.8A Pending CN104548204A (en) | 2013-10-25 | 2013-10-25 | Method for preparing fat granular filler |
Country Status (1)
| Country | Link |
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| CN (1) | CN104548204A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109106983A (en) * | 2018-10-31 | 2019-01-01 | 郑岩 | A kind of fat transfer material and its preparation method and application |
| CN110237305A (en) * | 2019-07-16 | 2019-09-17 | 成都清科生物科技有限公司 | A kind of filling preparation and preparation method thereof |
-
2013
- 2013-10-25 CN CN201310508120.8A patent/CN104548204A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109106983A (en) * | 2018-10-31 | 2019-01-01 | 郑岩 | A kind of fat transfer material and its preparation method and application |
| CN109106983B (en) * | 2018-10-31 | 2021-04-16 | 郑岩 | Fat transplantation material and preparation method and application thereof |
| CN110237305A (en) * | 2019-07-16 | 2019-09-17 | 成都清科生物科技有限公司 | A kind of filling preparation and preparation method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150429 |
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| WD01 | Invention patent application deemed withdrawn after publication |