CN109106983A - A kind of fat transfer material and its preparation method and application - Google Patents
A kind of fat transfer material and its preparation method and application Download PDFInfo
- Publication number
- CN109106983A CN109106983A CN201811283679.4A CN201811283679A CN109106983A CN 109106983 A CN109106983 A CN 109106983A CN 201811283679 A CN201811283679 A CN 201811283679A CN 109106983 A CN109106983 A CN 109106983A
- Authority
- CN
- China
- Prior art keywords
- transfer material
- fat
- fat transfer
- preparation
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000463 material Substances 0.000 title claims abstract description 102
- 238000012546 transfer Methods 0.000 title claims abstract description 89
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 210000001519 tissue Anatomy 0.000 claims abstract description 39
- 238000003756 stirring Methods 0.000 claims abstract description 24
- 239000013049 sediment Substances 0.000 claims abstract description 19
- 210000000577 adipose tissue Anatomy 0.000 claims abstract description 13
- 238000005119 centrifugation Methods 0.000 claims abstract description 7
- 238000004804 winding Methods 0.000 claims abstract description 6
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 5
- 230000003416 augmentation Effects 0.000 claims description 20
- 102000008186 Collagen Human genes 0.000 claims description 15
- 108010035532 Collagen Proteins 0.000 claims description 15
- 229920001436 collagen Polymers 0.000 claims description 15
- 210000000130 stem cell Anatomy 0.000 claims description 14
- 230000002792 vascular Effects 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 claims description 12
- 238000002435 rhinoplasty Methods 0.000 claims description 11
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 6
- 210000001789 adipocyte Anatomy 0.000 claims description 4
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 102000012422 Collagen Type I Human genes 0.000 claims description 2
- 108010022452 Collagen Type I Proteins 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000003716 rejuvenation Effects 0.000 claims description 2
- 231100000241 scar Toxicity 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 206010011409 Cross infection Diseases 0.000 abstract description 3
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 description 13
- 238000000034 method Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- 210000003734 kidney Anatomy 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 238000011049 filling Methods 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 241000699660 Mus musculus Species 0.000 description 4
- 229940030225 antihemorrhagics Drugs 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000000025 haemostatic effect Effects 0.000 description 4
- 238000011580 nude mouse model Methods 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 210000000845 cartilage Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000036558 skin tension Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- QTCANKDTWWSCMR-UHFFFAOYSA-N costic aldehyde Natural products C1CCC(=C)C2CC(C(=C)C=O)CCC21C QTCANKDTWWSCMR-UHFFFAOYSA-N 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000004728 ear cartilage Anatomy 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007972 injectable composition Substances 0.000 description 1
- ISTFUJWTQAMRGA-UHFFFAOYSA-N iso-beta-costal Natural products C1C(C(=C)C=O)CCC2(C)CCCC(C)=C21 ISTFUJWTQAMRGA-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a kind of fat transfer material and its preparation method and application, which includes the following steps: that (a) chooses lipochondrion, middle layer adipose tissue is collected by centrifugation；(b) adipose tissue is stirred using stirring rod, and collects the fibr tissue being wrapped in stirring rod, repeat above-mentioned stirring and collect operation, until stirring rod stir after without the fibr tissue of winding until；(c) fibr tissue of collection and physiological saline are mixed, collect sediment；(d) sediment of collection removed into moisture removal, be broken into starchiness up to fat transfer material；The preparation method is easy to operate, rapidly and efficiently, and without using exogenous agents, avoids cross-infection, and improve the yield of fat transfer material.
Description
Technical field
The present invention relates to lift face packing material technical fields, and in particular to a kind of fat transfer material and preparation method thereof and
Using.
Background technique
Recently as asking beautiful person more and more, augmentation rhinoplasty, the operation of chin augmentation also increase year by year.It is grand to be clinically applied to augmentation rhinoplasty
There are many packing material of chin art, and in synthetic material, solid has silicon rubber, expanded PTFE etc., these materials hold
The complication such as rejection, postoperative infection easily occur；Software filler has collagen, hyaluronic acid etc., these materials are can
Absorbing material needs to fill repeatedly, increases expense.There are dermal flap, Auricular cartilage, costal cartilage etc., autologous tissue in autologous tissue
Valve or cartilage transplantation bring the secondary wound and deformity of donor site.
Fat granules are as a kind of bulking of soft tissue, and due to its abundance, materials are easy, easy to operate, fill
The advantages that shape that is full of is good, without rejection, the increasingly attention by domestic and international shaping and beauty doctor.Because effect is obvious, damage
It is smaller, expense is lower, carry out Grainy fat tissue filling face asks beautiful person to increase year by year, but there are certain suctions after fat transfer
Yield, so that part asks beautiful person to need repeatedly filling, especially at the positions such as bridge of the nose, chin portion, because local organization amount is few, rete vasculosum
It does not enrich, the factors such as skin tension is big, the fat survival after leading to transplanting is lower, is extremely difficult to ideal effect.
Currently, disclosing a kind of Adipose Tissue gel in the prior art, which is being made
During standby, need for lipochondrion emulsification to be centrifuged again, however fat stem cell leads to fat by considerable damage in emulsion process
Survival rate after tissue stem cell gel yield and transplanting is lower；Moreover, containing a large amount of cells in Adipose Tissue gel
Fragment, these cell fragments are easily by own absorption, and absorptivity is higher when causing Adipose Tissue gel for augmentation rhinoplasty chin augmentation；
In addition, the oiliness cellular content that fat cell releases after being destroyed, is not worth and is thrown away, cause the wasting of resources.
Summary of the invention
The first object of the present invention is to provide a kind of preparation method of fat transfer material, preparation method operation letter
Singly, rapidly and efficiently, and without using exogenous agents, cross-infection is avoided, and improve the yield of fat transfer material.
The second object of the present invention is to provide a kind of fat transfer material, which is prepared by the above method
It obtains, the survival rate after fat transfer material transplanting is higher, and has lower absorptivity.
Third of the present invention is designed to provide a kind of application of fat transfer material, especially by fat transfer material grand
The application at the positions such as nose, chin augmentation, and reached higher survival rate.
Above-mentioned purpose to realize the present invention, the following technical scheme is adopted:
First aspect present invention provides a kind of preparation method of fat transfer material, which includes the following steps:
(a) it chooses lipochondrion, middle layer adipose tissue is collected by centrifugation；
(b) adipose tissue is stirred using stirring rod, and collects the fibr tissue being wrapped in stirring rod, in repetition
State stirring and collect operation, until stirring rod stir after without the fibr tissue of winding until；
(c) fibr tissue of collection and physiological saline are mixed, collect sediment；
(d) sediment of collection removed into moisture removal, be broken into starchiness up to fat transfer material.
Preferably, it is aseptically carried out in fat transfer material preparation process of the present invention, and used reagent,
Instrument carries out sterilization processing in advance.
The fibr tissue in lipochondrion can be sufficiently separated and be come by above-mentioned preparation method, and it is dry to avoid fat
The destruction of cell improves the yield of fat transfer material；The preparation method avoids intersecting and feel without using exogenous agents
Dye, and it is easy to operate, rapidly and efficiently, it is readily produced.
Further, the preparation method further includes that the fat transfer material merging that will be obtained uses nanometer adipose conversion device
It in two syringes of connection, pushes and pulls 2-3 times, removal oil shreds the fat transfer material purified.By being moved to fat
Plant material material carries out purification process, further improves collagen content, plasticity ability and support strength after improving augmentation rhinoplasty chin augmentation.
It can be realized in the present invention and fat made full use of, obtained fat transfer material is used for bridge of the nose, chin portion
Equal privileged sites filling, and remaining Grainy fat tissue can be used for filling out for the other positions of face or chest etc. after extracting rouge collagen
It fills.
In the present invention stringent limitation is not done to the source position of lipochondrion, it is preferable that rouge in technical scheme
Fat particle is selected from stomach fat；By selecting specific stomach fat, glue in the fat transfer material being prepared can be improved
The content of fibrinogen is unlikely to deform it conducive to the plasticity ability for improving fat transfer material.
Further, in the step (a), centrifugal rotational speed 900-1100r/min；Centrifugation time is 2-4min.Pass through
The setting of centrifugation can promote the separation of fibr tissue, be not easy to separate fibr tissue however, centrifugal rotational speed is lower, higher to cause
The fracture of fibr tissue is unfavorable for the extraction of subsequent fiber tissue, directly affects the yield of fat transfer material；The present invention passes through
Specific restriction to centrifugal condition, can be improved being sufficiently separated for fibr tissue, conducive to the fat transfer material for improving preparation
Yield.
Further, in the step (b), the type of stirring rod does not do stringent limitation, for example, can be the normal of this field
Advise stirring rod, or greasing pipe or haemostatic clamp；Preferably 18G greasing pipe.
Further, in the step (c), the volume ratio of physiological saline and lipochondrion is 1: (2-4).Pass through physiology salt
The addition of water and the restriction of additive amount, can remove the fat in fibr tissue, avoid the residual of fat, the fatty quilt after transplanting
Autologous tissue absorbs, and causes the absorptivity of fat transfer material higher.
Further, in the step (d), removing moisture removal is the moisture sucked on sediment using gauze；It is broken for adopting
It is shredded with scissors.
Second aspect of the present invention provides a kind of fat transfer material, which is prepared by above-mentioned preparation method
It arrives.Survival rate after fat transfer material transplanting is higher, and has lower absorptivity.
Further, the fat transfer material includes stromal vascular segment cell, fat stem cell and collagen.Study table
It is bright, fat transfer material after transplanting can be preferably improved by the collective effect of stromal vascular segment cell and fat stem cell
Survival rate；In addition, a large amount of collagenous fibres contained in fat transfer material, density is big, and viscosity is high, has stronger support
Power, have stronger plastotype ability, be unlikely to deform with it is too fat to move.
Preferably, the stromal vascular segment cell content is (6.2-9.5) × 106A/ml；The fat is dry thin
Born of the same parents' content is (1.4-3.1) × 106A/ml；Collagen content is 74%-98%.The present invention passes through to each in fat transfer material
The restriction of component content can preferably improve the survival rate of fat transfer material after transplanting.
Preferably, the collagen is Type I collagen.
Third aspect present invention provides a kind of fat transfer material in augmentation rhinoplasty, chin augmentation, augmentation rhinoplasty labial groove, Long Leigou, grand recess
Application in property scar or face rejuvenation.
Further, application of the fat transfer material in augmentation rhinoplasty or chin augmentation.Fat transfer material of the present invention is answered
The positions such as the bridge of the nose or chin portion that, rete vasculosum few used in tissue mass be not abundant, skin tension is big, and reached fat transfer after transplanting
Material survival rate with higher.
Compared with prior art, beneficial effect of the present invention includes at least:
(1) preparation method of fat transfer material of the present invention is easy to operate, rapidly and efficiently, and without using exogenous examination
Agent avoids cross-infection, and improves the yield of fat transfer material.
(2) fat transfer material of the present invention passes through the common of the stromal vascular segment cell of certain content and fat stem cell
Effect can preferably improve the survival rate of fat transfer material after transplanting.
(3) fat transfer material of the present invention contains a large amount of collagenous fibres, and density is big, and viscosity is high, has stronger support
Power so that fat transfer material have stronger plastotype ability, and be unlikely to deform with it is too fat to move.
(4) lipochondrion that the present invention uses is selected from own abdomen fat, can be improved the fat transfer material being prepared
The content of collagenous fibres in material is unlikely to deform it conducive to the plasticity ability for improving fat transfer material.
(5) fat transfer material of the present invention can be applied at positions such as augmentation rhinoplasty, chin augmentations, and reach fat transfer after transplanting
Material survival rate with higher.
(6) the application preparation method does not destroy lipochondrion, and after extracting fibr tissue, remaining lipochondrion can be used for
Face or chest filling, realize making full use of for resource.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products obtained can be bought by city.
Embodiment 1
The present embodiment is a kind of preparation method of fat transfer material, which includes the following steps:
(a) stomach fat particle 100ml is chosen, and is centrifuged 2min under 1000r/min revolving speed, then, collects middle layer rouge
Fat tissue is put in medicine bowl to sterile；
(b) adipose tissue is stirred using 18G greasing pipe, and collects the fibr tissue being wrapped on 18G greasing pipe
To in sterile kidney basin, repeating above-mentioned stirring and collect operation, until 18G greasing pipe stir after without the fibr tissue of winding until；
(c) 50ml sterile saline is added in sterile kidney basin and is stirred, collect sediment；
(d) sediment of collection is placed on sterile gauze and sucks moisture, then be placed in sterile small medicine glass, it will be fine with scissors
Tissue shear is tieed up into starchiness；
(e) starchiness sediment is placed in two 10ml syringes using the connection of nanometer fat converter, push-and-pull 2
It is secondary, then sediment after processing is set and sucks oil on gauze, fibr tissue is removed with tweezers and is shredded up to fat transfer material.
The yield for weighing the fat transfer material that the above method is prepared, and calculating fat transfer material is 11.8%；
And using degreasing, collagenase digesting, centrifugation, cell count and flow cytometry to the above-mentioned fat transfer material being prepared
It is detected, wherein stromal vascular segment cell content is 9.5 × 106A/ml；Fat stem cell content is 2.1 × 106A/
ml；Collagen content is 91%.
Embodiment 2
The present embodiment is a kind of preparation method of fat transfer material, which includes the following steps:
(a) stomach fat particle 200ml is chosen, and is centrifuged 4min under 1000g/min revolving speed, then, collects middle layer rouge
Fat tissue is put in medicine bowl to sterile；
(b) adipose tissue is stirred using haemostatic clamp, and collects the fibr tissue that is wrapped on haemostatic clamp to sterile
In kidney basin, repeat above-mentioned stirring and collect operation, until haemostatic clamp stir after without the fibr tissue of winding until；
(c) 50ml sterile saline is added in sterile kidney basin and is stirred, collect sediment；
(d) sediment of collection is placed on sterile gauze and sucks moisture, then be placed in sterile small medicine glass, it will be fine with scissors
Tissue shear is tieed up into starchiness；
(e) starchiness sediment is placed in two 10ml syringes using the connection of nanometer fat converter, push-and-pull 3
It is secondary, then sediment after processing is set and sucks oil on gauze, fibr tissue is removed with tweezers and is shredded up to fat transfer material.
The yield for weighing the fat transfer material that the above method is prepared, and calculating fat transfer material is 11.5%；
And the above-mentioned fat transfer material being prepared is detected using the detection method of embodiment 1, wherein stromal vascular segment
Cell content is 6.2 × 106A/ml；Fat stem cell content is 1.4 × 106A/ml；Collagen content is 92%.
Embodiment 3
The present embodiment is a kind of preparation method of fat transfer material, which includes the following steps:
(a) stomach fat particle 150ml is chosen, and is centrifuged 3min under 1000g/min revolving speed, then, collects middle layer rouge
Fat tissue is put in medicine bowl to sterile；
(b) adipose tissue is stirred using 18G greasing pipe, and collects the fibr tissue being wrapped on 18G greasing pipe
To in sterile kidney basin, repeating above-mentioned stirring and collect operation, until 18G greasing pipe stir after without the fibr tissue of winding until；
(c) 50ml sterile saline is added in sterile kidney basin and is stirred, collect sediment；
(d) sediment of collection is placed on sterile gauze and sucks moisture, then be placed in sterile small medicine glass, it will be fine with scissors
Tissue shear is tieed up into starchiness；
(e) starchiness sediment is placed in two 10ml syringes using the connection of nanometer fat converter, push-and-pull 2
It is secondary, then sediment after processing is set and sucks oil on gauze, fibr tissue is removed with tweezers and is shredded up to fat transfer material.
The yield for weighing the fat transfer material that the above method is prepared, and calculating fat transfer material is 12.3%；
And the above-mentioned fat transfer material being prepared is detected using the detection method of embodiment 1, wherein stromal vascular segment
Cell content is 7.7 × 106A/ml；Fat stem cell content is 1.5 × 106A/ml；Collagen content is 95%.
Embodiment 4
The present embodiment is a kind of preparation method of fat transfer material, the preparation method base of the preparation method and embodiment 3
This is identical, and difference, which is only that, replaces with leg lipochondrion for step (a) midriff lipochondrion.
The yield for weighing the fat transfer material that the above method is prepared, and calculating fat transfer material is 9.4%；And
The above-mentioned fat transfer material being prepared is detected using the detection method of embodiment 1, wherein stromal vascular segment is thin
Born of the same parents' content is 6.4 × 106A/ml；Fat stem cell content is 3.1 × 106A/ml；Collagen content is 94%.
Reference examples 1
This reference examples is a kind of fat transfer material containing highly concentrated adipose-derived stem cells and extracellular matrix, should
Fat transfer material by embodiment 1 in the Chinese patent application file according to Publication No. CN 106178134A preparation method
It is prepared, and lipochondrion is selected from stomach fat.
The yield for weighing the fat transfer material that the above method is prepared, and calculating fat transfer material is 10.3%；
And the above-mentioned fat transfer material being prepared is detected using the detection method of embodiment 1, wherein stromal vascular segment
Cell content is 3.4 × 106A/ml；Fat stem cell content is 1.5 × 106A/ml；Collagen content is 51%.
Experimental example
Experimental material:
Choose the healthy nude mice of 12 weight 20-25g, 5-7 week old；
The abdomen and leg adipose tissue for choosing same patient, according to the preparation side of above-described embodiment 2,3,4 and reference examples 1
Fat transfer material is prepared in method respectively；
Experimental method:
It is the fat transfer material that embodiment 2 is prepared that experiment, which is divided into 4 groups: A group,；B group is what embodiment 3 was prepared
Fat transfer material；C group is the fat transfer material that embodiment 4 is prepared；D group is that the fat that reference examples 1 are prepared moves
Plant material material.
Above-mentioned 4 groups of ingredients are injected in the experiment area nude mice the small of the back subcutaneous four Ge Shou respectively according to randomization, respectively
Group component injection amount is 300mg；Then, experiment nude mice is subjected to raising 3 months under the same conditions.
Transplant fat graft materials are taken out to the nude mice after raising 3 months, weight in wet base, and root are measured using electronic balance immediately
Be calculated the average survival of 4 groups of injectable compositions according to survival rate=average weight in wet base/injection volume × 100%, concrete outcome referring to
Table 1；Then volume size, quality, color and the vascular distribution situation for observing fat transfer material observe situation referring to table 1；
Table 1
As shown in Table 1:
There is highest survival rate using the fat transfer material of 2 preparation method of the embodiment of the present application preparation arrived, and
By experimental example data it is found that stromal vascular segment cell, fat stem cell and collagen content survive fat transfer material
Rate has synergistic effect, and can determine the absorption of fat transfer material prepared by the embodiment of the present application 2 by view volume size
Rate is minimum, and is significantly better than the fat transfer material that reference examples 1 are prepared.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (10)
1. a kind of preparation method of fat transfer material, which comprises the steps of:
(a) it chooses lipochondrion, middle layer adipose tissue is collected by centrifugation；
(b) adipose tissue is stirred using stirring rod, and collects the fibr tissue being wrapped in stirring rod, repeat above-mentioned stir
Mix and stir collection operation, until stirring rod stir after without the fibr tissue of winding until；
(c) fibr tissue of collection and physiological saline are mixed, collect sediment；
(d) sediment of collection removed into moisture removal, be broken into starchiness up to fat transfer material.
2. preparation method according to claim 1, which is characterized in that the preparation method further includes that the fat that will be obtained moves
Plant material material merging using nanometer adipose conversion device connection two syringes in, push and pull 2-3 time, remove oil, shred to obtain it is pure
The fat transfer material of change.
3. preparation method according to claim 1, which is characterized in that in the step (a), lipochondrion is selected from abdomen rouge
Fat；Centrifugal rotational speed is 900-1100r/min；Centrifugation time is 2-4min.
4. preparation method according to claim 1, which is characterized in that in the step (c), physiological saline and lipochondrion
Volume ratio be 1: (2-4).
5. a kind of fat transfer material, which is characterized in that be prepared by any preparation method of claim 1-3.
6. fat transfer material according to claim 4, which is characterized in that the fat transfer material includes stromal vascular
Segment cell, fat stem cell and collagen.
7. fat transfer material according to claim 6, which is characterized in that the stromal vascular segment cell content is
(6.2-9.5)×106A/ml；The fat stem cell content is (1.4-3.1) × 106A/ml；Collagen content is 74%-
98%.
8. fat transfer material according to claim 6, which is characterized in that the collagen is Type I collagen.
9. any fat transfer material of claim 4-8 is in augmentation rhinoplasty, chin augmentation, augmentation rhinoplasty labial groove, Long Leigou, grand pitting scar
Application in trace or face rejuvenation.
10. application of any fat transfer material of claim 4-8 in augmentation rhinoplasty or chin augmentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811283679.4A CN109106983B (en) | 2018-10-31 | 2018-10-31 | Fat transplantation material and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811283679.4A CN109106983B (en) | 2018-10-31 | 2018-10-31 | Fat transplantation material and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109106983A true CN109106983A (en) | 2019-01-01 |
| CN109106983B CN109106983B (en) | 2021-04-16 |
Family
ID=64855942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811283679.4A Active CN109106983B (en) | 2018-10-31 | 2018-10-31 | Fat transplantation material and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109106983B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109646078A (en) * | 2019-02-21 | 2019-04-19 | 郑岩 | A kind of collagenous fibres extracting tool |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014207135A1 (en) * | 2013-06-26 | 2014-12-31 | Stemform Aps | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation |
| CN104548204A (en) * | 2013-10-25 | 2015-04-29 | 西安莲湖美立方医院有限公司 | Method for preparing fat granular filler |
| CN106178134A (en) * | 2016-07-14 | 2016-12-07 | 鲁峰 | A kind of adipose-derived stem cells containing high enrichment and the fat transplantation material of extracellular matrix and pure physical preparation method thereof and application |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN107224614A (en) * | 2017-05-11 | 2017-10-03 | 黎洪棉 | A kind of preparation and its clinical practice of the Adipose-derived stromal cells cograft material rich in cell factor |
-
2018
- 2018-10-31 CN CN201811283679.4A patent/CN109106983B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014207135A1 (en) * | 2013-06-26 | 2014-12-31 | Stemform Aps | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation |
| CN104548204A (en) * | 2013-10-25 | 2015-04-29 | 西安莲湖美立方医院有限公司 | Method for preparing fat granular filler |
| CN106178134A (en) * | 2016-07-14 | 2016-12-07 | 鲁峰 | A kind of adipose-derived stem cells containing high enrichment and the fat transplantation material of extracellular matrix and pure physical preparation method thereof and application |
| CN106421920A (en) * | 2016-11-16 | 2017-02-22 | 沈阳细胞治疗工程技术研发中心有限公司 | Fat filler and preparation method thereof |
| CN107224614A (en) * | 2017-05-11 | 2017-10-03 | 黎洪棉 | A kind of preparation and its clinical practice of the Adipose-derived stromal cells cograft material rich in cell factor |
Non-Patent Citations (2)
| Title |
|---|
| 姚尧: "SVF-gel：一种高浓度脂肪来源干细胞基质胶的实验研究及临床应用", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 李青峰主编: "《自体脂肪移植技术》", 30 November 2014, 军事医学科学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109646078A (en) * | 2019-02-21 | 2019-04-19 | 郑岩 | A kind of collagenous fibres extracting tool |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109106983B (en) | 2021-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3559566B2 (en) | Use of autologous dermal fibroblasts for repair of skin and soft tissue defects | |
| US20110002895A1 (en) | Composition for autotransplantation or allotransplantation using dental pulp stem cell, and use of the composition | |
| RU2660561C2 (en) | Use of ex-vivo adipose tissue-derived stem cells for cosmetic breast lipofilling or for facial lipofilling and/or rejuvenation | |
| KR101121154B1 (en) | A composition for transplant comprising adipose stem cells or adipocytes | |
| Johnson | Body contouring by macroinjection of autogenous fat | |
| BRPI0710174A2 (en) | composition for periodontal soft tissue regeneration and method for producing it | |
| CN102068318B (en) | Manufacturing method of biological tooth root bracket material | |
| CN112587729B (en) | Bone repair material | |
| BR112020015616B1 (en) | BIOINK COMPOSITION FOR DERMIS REGENERATION SHEET, METHOD FOR MANUFACTURING CUSTOM DERMIS REGENERATION SHEET USING THE SAME AND CUSTOM DERMIS REGENERATION SHEET MANUFACTURED USING THE MANUFACTURING METHOD | |
| US20200390930A1 (en) | Systems and methods for treating a wound with wound packing | |
| Shukla et al. | Acellular dermis as a dermal matrix of tissue engineered skin substitute for burns treatment | |
| US9956317B2 (en) | Clinical applications of formulations containing adipose-derived stem cells | |
| de Benito et al. | Treatment of depressed scars with a dissecting cannula and an autologous fat graft | |
| Eshghpour et al. | Platelet-rich fibrin: An autologous fibrin matrix in surgical procedures: A case report and review of literature | |
| CN109106983A (en) | A kind of fat transfer material and its preparation method and application | |
| Costanzo et al. | Autologous fat grafting in plastic and reconstructive surgery: An historical perspective | |
| CN109846902B (en) | Preparation method of heterogenous platelet-rich gel factor | |
| KR101719743B1 (en) | Method for obtaining stromal vascular fraction from adipose tissue | |
| US20180258380A1 (en) | A method of culturing cells | |
| CN112618798B (en) | Preparation method of bone repair material | |
| KR100956267B1 (en) | A semi-floating composition for transplant comprising elastin as a stabilizing agent | |
| CN111921014A (en) | Rehmannia polysaccharide/heterogenous calcined bone composite bone repair material | |
| Bilkay et al. | Augmentation of the calf region with autologous fat and platelet-rich plasma-enhanced fat transplants: a comparative study | |
| Tiryaki et al. | Stem Cell-Enriched Fat Injection in Aesthetic, Reconstructive Breast Surgery | |
| Cigna et al. | Grafts in plastic surgery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210719 Address after: 610000 room 2412, floor 24, building 300, jiaozi Avenue, Chengdu hi tech Zone, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan Patentee after: Sichuan kulijun Technology Co.,Ltd. Address before: 610041 No.25, South Section 3, 2nd Ring Road, Wuhou District, Chengdu, Sichuan Province Patentee before: Zheng Yan |
|
| TR01 | Transfer of patent right |