CN104543391A - Preparation method of liquid feed additive - Google Patents
Preparation method of liquid feed additive Download PDFInfo
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- CN104543391A CN104543391A CN201410831174.2A CN201410831174A CN104543391A CN 104543391 A CN104543391 A CN 104543391A CN 201410831174 A CN201410831174 A CN 201410831174A CN 104543391 A CN104543391 A CN 104543391A
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Abstract
The invention relates to a preparation method of liquid feed additive. The preparation method is characterized by comprising the following steps: collecting waste liquid flowing out after ginkgetin is adsorbed from ginkgo leaf boiling extract in the processing of ginkgo leaf extract through macroporous adsorption resin, concentrating into a concentrated solution with the specific gravity of 1.25, adding oligosaccharide according to the weight ratio of the concentrated solution and the oligosaccharide being 1: 0.05-0.1, putting in a fermentation tank, heating to 100 DEG C and boiling for 1 h, cooling to below 35 DEG C to obtain a fermentation medium, then adding 500-1000 billions of enterococcus faecalis, 500-1000 billions of bacteriophagia vibrio and 500-1000 billions of brewer's yeast to 1 kg of culture medium, carrying out aerobic culture for 24-36 h under the condition of 27-30 DEG C to obtain a fermentation culture solution, quantitatively subpackaging in a glass or plastic bottle after sterilization, sealing the bottle opening, and packaging to obtain the product. A novel method and a novel technological and technical route are provided for the utilization of the waste liquid generated in the extraction and production of ginkgo leaf, and feasibility is provided for waste utilization.
Description
Technical field
The invention belongs to feed additive field, be specifically related to a kind of preparation method of liquid feed additive.
Background technology
Along with the scientific and technological progress of livestock and poultry breeding industry, the theory of animal-breeding is supplemented by the full nutrition that the past is single, the Mode change of the various nutritional factors of balance required for animal growth is on the balanced basis of Animal nutrition key element, regulate animal body microbial balance, prevent disease is carried out to harmful microbe suppression in enteron aisle or by strengthening non-specific immune function by strengthening body, promote growth of animal, pass through gastral conditioning simultaneously, balanced nutrients key element is more effectively absorbed, alimentary canal environment is the key effectively absorbed, this just needs one and can effectively absorb during balanced nutrients, improve food conversion ratio, improve immunity of organisms, strengthen the resistance against diseases of animal to reduce comprehensive effect of the generation of disease, animal is made to reach healthy growth, thus improve the economic benefit of livestock and poultry breeding industry.
Summary of the invention
The object of the invention is the waste liquid produced after macroporous resin adsorption by the leach cooking liquid of ginkgo leaf in ginkgo biloba p.e (GBE) production process, through concentrated concentrate processed, a kind of liquid feed additive is made in further employing probiotic's culture fermentation, this additive not only effectively can regulate the micro-raw group of animal intestinal, maintain microecological balance, strengthen non-specific immune function so that arrive prevent disease, promote growth of animal, simultaneously can optimizing animal alimentary canal microorganism species, promote beneficial bacterium breeding and improve gastrointestinal mucosal state, whet the appetite, improve absorbing of various nutriment.
The object of the present invention is to provide a kind of preparation method of liquid feed additive.
To achieve these goals, the present invention specifically have employed following technical scheme:
A preparation method for liquid feed additive, is characterized in that being made up of following steps:
(1) collect the waste liquid that in ginkgo biloba p.e processing, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin absorption, temperature 50-85 DEG C, under vacuum 0.05-0.1MPa condition, being concentrated into proportion is 1.25, obtains ginkgo leaf waste liquid concentrate;
(2) the ginkgo leaf waste liquid concentrate (1) obtained adds functional oligose, regulates pH to 6.6-7.0 to make slip; The wherein addition of functional oligose is 1:0.05-0.1 ratio in the weight ratio of ginkgo leaf waste liquid concentrate and functional oligose;
(3), in the slip impouring fermentation tank (2) obtained, heating is boiled to 100 DEG C and is boiled 1 hour, and then allowing it naturally cool to less than 35 DEG C, is fermentation medium;
(4) fermentation medium (3) obtained adds the enterococcus faecalis of 5000 hundred million-10000 hundred million by fermentation medium weight 1kg, the saccharomyces cerevisiae eating into vibrios and 5000 hundred million-10000 hundred million addicted to bacterium of 5000 hundred million-10000 hundred million, ventilate cavity under 27-30 DEG C of condition, cultivate 24-36 hour, obtain fermentation culture;
(5) by fermentation culture quantitative separating obtained to (4) in through sterilization treatment and in dried vial or plastic bottle, sealing bottleneck, packs to obtain product.
In above-mentioned steps (1), preferred concentrated condition is 60 DEG C-75 DEG C, 0.06-0.08MPa.
Concentrated described in above-mentioned steps (1) selects vacuum rotary evaporator or plate evaporation inspissator.
Functional oligose structure described in step described above (2) is 2-10 monose composition, is specially galactooligosaccharide, mannan-oligosaccharides, xylo-oligosaccharide, FOS, chitosan oligomer, malto-oligosaccharide, soyabean oligosaccharides and Tang oligosaccharide; Its ratio is 3-6:0-4:0-3:0-2:0-2:0-3:0-2:0-2.
Described enterococcus faecalis, can to use select the freeze-dried powder goods of thalline addicted to bacterium moth vibrios and saccharomyces cerevisiae according to reality, the weight proportion of its thalline dry powder and other proportion materials is determined by the dry powder of thalline and the thalline content of final products.
Described enterococcus faecalis, can choice for use single culture preparation commercially available addicted to the dry powder goods of bacterium moth vibrios and saccharomyces cerevisiae, also existing method can be adopted to prepare, such as enterococcus faecalis, can be cultivated respectively by liquid fermentation equipment addicted to bacterium moth vibrios and the dry powder goods of saccharomyces cerevisiae, then with supercentrifuge, the thalline in zymotic fluid is separated, the drying of bacterium mud obtained by vacuum freeze drying mode, the viable bacteria concentration of the thalline dry powder of acquisition exceedes every gram 10,000,000,000.
The condition of the ventilate cavity described in above-mentioned steps (4) is throughput is 1:0.3V/V/min.
The above-described equipment of the present invention is this area conventional equipment.
Do not state the operation of its technical process in processing step in the present invention in detail, be the routine operation in the general knowledge of those skilled in the art.Such as ventilate cavity passes into filtrated air etc.
Ratio described in the present invention is apart from specified otherwise, and the percentage described in other is all weight percentage.
The present invention utilizes enterococcus faecalis, cultivates addicted to bacterium moth vibrios and saccharomyces cerevisiae symbiosis, and wherein enterococcus faecalis can form biofilm and is attached on animal intestine mucous membrane in enteron aisle, and grow, Growth and reproduction.Enterococcus faecalis by the fiber deliquescing in feed, can be improved food conversion ratio.Enterococcus faecalis can produce multiple antibacterial material, and these antibacterial materials have good inhibitory action to pathogenic bacteria such as salmonella, Escherichia coli and staphylococcus aureuses.Have scholar's research to show, enterococcus faecalis can produce Enterocin, effectively can suppress Listeria and staphylococcus aureus and putrefactive microorganisms growth and breeding.In addition, be a kind of Grain-negative comma bacillus of schizomycetotrophy addicted to bacterium moth vibrios, very similar to bacteriophage addicted to bacterium characteristic.Study proof, addicted to bacterium moth vibrios energy cracking swine escherichia coli, cholera fowl detection of Salmonella, white diarrhea Salmonella typhi, can be used for fowl bacterial diarrhea prevention and therapy (Shi Bo edits. novel fodder additive development & application, Chemical Industry Press, Beijing in August, 2005 first edition, 116 pages of the 7th section of 2-5 are capable).Yeast cell wall main component is glucan, containing rich in protein, nucleic acid, carbohydrate, lipid material, mineral matter and B family vitamin such as Cobastab in cell
1, Cobastab
6, Cobastab
12and the physiological activator such as wheat steroid ethanol, nicotinic acid, folic acid, pantothenic acid, inositol, can promote that animal digestion absorbs, improve animal appetite, obtained product is cultivated in its symbiosis, after test, show the growth rate that can improve livestock and poultry, and it is still feeding nutritional agents and feed addictive not, also be feeding therapeutic agent, partly can replace the treatment of antibiotic disease of digestive system.Give to feed raise through admixing liquid feed additive of the present invention to the trouble of 30 body weight 35-40 kilogram weights diarrhoea piglet by feed relative 3%, cure rate reached 75-80% on 1st, and there is diarrhoea piglet 24 antibiotic conventional therapy in control group, cure rate on the two is 68-76%, and test shows that liquid feed additive of the present invention has good effect to grice diarrhoea.
Ginkgo leaf has very high nutritive value and medical value, containing active ingredients such as 46 kinds of flavone compounds and terpene, phenols, trace element and amino acid, its nutrient composition content is also very abundant, in butt, protein 10.9% ~ 15.5% in ginkgo leaf, total reducing sugar 7.38% ~ 8.69%, reduced sugar 4.64% ~ 5.63%, vitamin C 66.78 ~ 129.20mg/100g, vitamin E 6.17 ~ 8.05 mg/100g, Cobastab
10.06 ~ 0.09 mg/100g, Cobastab
20.30 ~ 0.45 mg/100g, carrotene 14.52 ~ 18.80 mg/100g, choline 28.00 ~ 39.50 mg/100g.Various amino acid content: aspartic acid 1.26 ~ 1.73g/100g, threonine 0.50 ~ 0.72 g/100g, serine 0.55 ~ 0.72 g/100g, glutamic acid 1.16 ~ 1.79 g/100g, glycine 0.7 ~ 0.92 g/100g, alanine 0.71 ~ 1.09 g/100g, valine 0.64 ~ 0.99 g/100g, methionine 0.18 ~ 0.24 g/100g, isoleucine 0.44 ~ 0.63 g/100g, leucine 0.83 ~ 1.10 g/100g, tyrosine 0.37 ~ 0.56 g/100g, phenylalanine 0.60 ~ 0.83 g/100g, GABA 0.18 ~ 0.34 g/100g, histidine 0.23 ~ 0.34 g/100g, lysine 0.80 ~ 1.01 g/100g, tryptophan 0.21 ~ 0.23 g/100g, arginine 0.60 ~ 0.91 g/100g, proline 0.69 ~ 1.28 g/100g, total amino acid 10.73 ~ 15.43 g/100g, essential amino acid 4.45 ~ 6.04 g/100g, wherein essential amino acid/total amino acid 39.14 ~ 41.47%, essential amino acid/nonessential amino acid 64.46 ~ 70.86%, various Mineral Elements Content: calcium 1860 ~ 2360mg/100g, phosphorus 298.10 ~ 407.10 mg/100g, iron 22.85 ~ 63.56 mg/100g, fluorine 6.00 ~ 13.00 mg/100g, copper 0.56 ~ 0.73 mg/100g, manganese 2.94 ~ 6.10 mg/100g, zinc 1.43 ~ 1.80 mg/100g, chromium <0.12 mg/100g, cobalt <0.12 mg/100g, boron 30.67 ~ 55.54 μ g/100g, selenium 5.45 ~ 15.44 μ g/100g.No matter being all not less than soybean protein, Chicken Albumin and WHO pattern from content with being worth with regard to essential amino acid in ginkgo leaf (butt) and the essential amino acid in high-quality protein, WHO model comparision (as following table) its ginkgo leaf, proving that its ginkgo leaf has very high nutritive value.
table: essential amino acid and high-quality protein, WHO model comparision in ginkgo leaf
Unit: g/100g (protein)
| Amino acid name | Albumen in ginkgo | Soybean protein | Chicken Albumin | WHO |
| Threonine | 44.5～50.5 | 37.0 | 47.0 | 9.0 |
| Valine | 55.8～64.1 | 48.0 | 66.0 | 13.0 |
| Methionine | 14.5～17.0 | 11.0 | 57.0 | 17.0 |
| Isoleucine | 36.4～40.8 | 49.0 | 54.0 | 13.0 |
| Leucine | 71.2～76.1 | 77.0 | 86.0 | 19.0 |
| Phenylalanine+tyrosine | 84.1～90.1 | 91.0 | 93.0 | 19.0 |
| Histidine | 21.0～22.0 | 25.0 | 22.0 | 16.0 |
| Lysine | 65.4～73.4 | 61.0 | 70.0 | 16.0 |
| Tryptophan | 13.6～21.1 | 14.0 | 17.0 | 5.0 |
But, it is only extract bilobanone that existing ginkgo leaf extracts processing, and the extracted amount of general flavone is only the 0.2-0.425% of the weight of ginkgo leaf, a large amount of beneficiating ingredients does not obtain rational and useful extraction and application yet, and ginkgo leaf decoction process water soluble beneficial composition soluble in water after by macroporous resin adsorption flavones along with the aqueous solution is discarded as waste water.Enrich people the mainstay industry in strong county in Jiangsu Pizhou City as ginkgo industry, the cultivated area of ginkgo, the output of ginkgo leaf and all leading whole nation of ginkgo leaf processing, the resource of ginkgo leaf is very abundant, gather in the crops dry Folium Ginkgo according to statistics every year and reach more than 4.6 ten thousand tons, the local bilobanone in Pizhou City extracts personal about 3.1 ten thousand tons of processing enterprise, at present, the enterprise of Pizhou City's production and processing ginkgo biloba p.e has 8, consume dry Folium Ginkgo every year with regard to Pizhou Xinyuan Biological Products Co., Ltd. and reach 10,000 tons, Xuzhou Tianli Biological Technology Co., Ltd. consumes dry Folium Ginkgo 0.5 ten thousand tons, bass special biological products Co., Ltd in Xuzhou consumes dry Folium Ginkgo 0.6 ten thousand tons, other enterprise's year wastage in bulk or weight dry Folium Ginkgos about 10,000 tons.
But, the beneficiating ingredient that the Aqueous extracts of decoction ginkgo leaf is most is leached simultaneously, the waste liquid flowed out after macroporous absorbent resin is to GINKGO BILOBA EXTRACT absorption, the content of its nutritional labeling is still higher than the beneficiating ingredient of ginkgo leaf residue, in existing ginkgo biloba p.e (GBE) is produced, it extracts the juice after flavones all by as waste discharge.According to existing production method ginkgo biloba p.e be by ginkgo leaf through pulverize, extract with alcohol heating reflux, merge extract, reclaim ethanol and be concentrated into appropriate, be added on processed good large pore resin absorption column, use the ethanol elution of water and variable concentrations successively, collect corresponding eluent, reclaim ethanol, spraying dry; Or recovery ethanol, be condensed into thick paste, vacuum drying, pulverize, obtained (Chinese Pharmacopoeia Commission's Pharmacopoeia of People's Republic of China, version in 2010, one, 392 pages).But, the manufacturer of existing ginkgo biloba p.e is all optimized the extraction process of ginkgo leaf, its technique many employings dry Folium Ginkgo is direct or pulverize rear boiling, extract, merge extract, be added in processed good macroporous resin column, adsorb, then carry out wash-out with the ethanol of water and variable concentrations successively.No matter adopt method technique or the Optimization Technology of enterprise of " pharmacopeia ", the extracted amount of ginkgo leaf total flavonoid extract is only at 0.2-0.425%(dry product meter) between, " pharmacopeia " is although define ginkgo leaf and calculate by dry product, must not 0.40% be less than containing total flavonoids, must not 0.25% be less than containing terpene lactone, but it is different with regard to the collecting season of ginkgo leaf, the difference of growing environment difference and processing and storage condition, the content difference of its bilobanone is also larger, but the extracted amount being no matter extract is much, and its maximum level extracted amount is all below 0.50%.But in the processing enterprise of ginkgo biloba p.e, in the process extracting flavonol glycosides, which enterprise of family a large amount of extracts, all by as discharging of waste liquid, does not have carry out recovery processing and utilization in the process of its ginkgo biloba p.e to its waste liquid at present.The liquid waste processing approach that current ginkgo leaf extracts processing enterprise has two, one is direct discharge, and to periphery soil, water resource pollutes is undoubtedly, and two is that unit with good conditionsi adopts sewage disposal system to carry out processing rear discharge, add financial burden, improving production cost is also undoubtedly.But, in the process that ginkgo biloba p.e (GBE) is produced, only just extract the general flavone composition of 0.2-0.425% by macroporous absorbent resin adsorbing and extracting, and other organic and inorganic useful nutritional labeling is all left in the aqueous solution as discharging of waste liquid, this pollutes not only can to environment and water source, a lot of beneficiating ingredients with better nutritivity value goes out of use, and causes the significant wastage of resource.
Beneficial features of the present invention:
(1) the present invention utilizes the waste liquid of ginkgo leaf decoction liquor after macroporous absorbent resin absorption, through concentrated obtained concentrate, nutritional labeling in its waste liquid obtains gathering utilization, ginkgo leaf waste liquid after adsorbing and extracting GBE is concentrated into the concentrate that proportion is 1.25 by the present invention, press GB/T6432 assay method respectively through 3 batches to detect, its result crude protein content is respectively 29.8%, 30.6% and 30.2%, mean value is 30.2%, as the fermentation medium of the liquid feed additive of probio, not only can improve its protein, amino acid equal size is that livestock and poultry cultivation has additional nutrients source, also effectively waste resource is used, increase economic benefit and environmental benefit.The present invention is that the biofermentation utilization of the waste liquid produced in ginkgo leaf extraction processing provides new Method and process, technology path, for the industrial operation of waste utilization provides feasibility, has good practical value and good economic benefit.
(2) product of the present invention can provide the matrix of beneficial bacterium effectively, change enteron aisle bacterium phase, serve as immuno-stimulator, improve antigen immune response ability, thus increase livestock and poultry body fluid and cellular immunity, be beneficial to the secretion of endogenous digestive ferment and the stable of the digested enzymatic activity of stomach and intestine, properer the cooperatively interacting of actual needs that abundant nutriment and strong food calling effect enable feed intake and human body grow, the profound growth potential excavating animal, improves production performance.
(3) functional oligose not only promotes the proliferation of probiotics in enteron aisle, plays humidification to the stable of product microorganism live bacteria, and the field planting of collaborative probio in enteron aisle and propagation, play good synergy, to feeding, object plays a role in health care.
(4) liquid feed additive of the present invention is water-soluble, makes an addition in animal drinking water and drinks for it, be beneficial to absorption and the utilization of animal, strengthens the effect of product.Product has certain viscosity, when mix feed, the thalline of the probio contained can be made to be attached on feed surface, is beneficial to bacterial classification performance effect and increases operation rate.
Detailed description of the invention
Further illustrating that the specific embodiment of the invention is made below in conjunction with embodiment; but not limitation of the present invention; to following preferred embodiment to one skilled in the art; under the prerequisite not departing from the present invention program's know-why, the improvements and modifications of carrying out all are considered as protection scope of the present invention.
Embodiment 1
Use corresponding Optimal Medium, enterococcus faecalis is individually cultivated, addicted to bacterium moth vibrios and saccharomyces cerevisiae by liquid fermentation equipment, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for centrifugal bacterium mud out, to obtain viable bacteria content be 20,000,000,000/gram enterococcus faecalis dry powder, viable bacteria content is 20,000,000,000/gram addicted to bacterium moth vibrios dry powder and viable bacteria content be 20,000,000,000/gram saccharomyces cerevisiae dry powder.
Embodiment 2
(1) waste liquid that in being processed by the ginkgo biloba p.e collected, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin absorption flavones, filter through 200 eye mesh screens, vacuum rotary evaporator is adopted to concentrate under temperature 60 C, vacuum 0.06MPa condition, be 1.25 to proportion, obtain ginkgo leaf waste liquid concentrate;
(2) get the ginkgo leaf waste liquid concentrate 100 kilograms that step (1) obtains, add by galactooligosaccharide: the functional oligose that Tang oligosaccharide=3:2 forms 5 kilograms, mixing and stirring, regulate pH to 6.7 to make slip;
(3) by the slip that step (2) is obtained, insert in fermentation tank, be heated to 100 DEG C and boil 1 hour, then allow it naturally cool to less than 35 DEG C, obtain fermentation medium;
(4) add in the fermentation medium obtained to step (3) every gram of viable bacteria content that embodiment 1 obtains be 20,000,000,000 enterococcus faecalis dry powder 5.25 kilograms, every gram viable bacteria content be 20,000,000,000 addicted to bacterium moth vibrios dry powder 5.25 kilograms and every gram viable bacteria content be 20,000,000,000 5.25 kilograms, saccharomyces cerevisiae dry powder, temperature be 30 DEG C, throughput be 1:0.3V/V/min condition under cultivate 24 hours, obtain fermentation culture;
(5) by fermentation culture obtained for step (4), quantitative separating is in through sterilization treatment and in dried vial, sealing bottleneck packaging, obtains product of the present invention.
Embodiment 3
(1) waste liquid that in being processed by the ginkgo biloba p.e collected, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin Adsorption For Ag flavone, filter through 200 eye mesh screens, vacuum rotary evaporator is adopted to concentrate under temperature 75 DEG C, vacuum 0.08MPa condition, be 1.25 to proportion, obtain ginkgo leaf waste liquid concentrate;
(2) the ginkgo leaf waste liquid concentrate 100 kilograms that step (1) obtains is got, add by galactooligosaccharide: mannan-oligosaccharides: xylo-oligosaccharide: the functional oligose that malto-oligosaccharide=3:2:2:3 forms 10 kilograms, mixing and stirring, regulates pH to 7.0 to make slip;
(3) by the slip that step (2) is obtained, insert in fermentation tank, be heated to 100 DEG C and boil 1 hour, then allow it naturally cool to less than 35 DEG C, obtain fermentation medium;
(4) add in the fermentation medium obtained to step (3) every gram of viable bacteria content that embodiment 1 obtains be 20,000,000,000 enterococcus faecalis dry powder 2.75 kilograms, every gram viable bacteria content be 20,000,000,000 addicted to bacterium moth vibrios dry powder 5.5 kilograms and every gram viable bacteria content be 20,000,000,000 2.75 kilograms, saccharomyces cerevisiae dry powder, temperature be 27 DEG C, throughput be 1:0.3V/V/min condition under cultivate 36 hours, obtain fermentation culture;
(5) by fermentation culture obtained for step (4), quantitative separating is in through sterilization treatment and in dried vial, sealing bottleneck packaging, obtains product of the present invention.
Embodiment 4
(1) waste liquid that in being processed by the ginkgo biloba p.e collected, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin absorption flavones, filter through 200 eye mesh screens, vacuum rotary evaporator is adopted to concentrate under temperature 70 C, vacuum 0.075MPa condition, be 1.25 to proportion, obtain ginkgo leaf waste liquid concentrate;
(2) the ginkgo leaf waste liquid concentrate 100 kilograms that step (1) obtains is got, add by galactooligosaccharide: mannan-oligosaccharides: chitosan oligomer: malto-oligosaccharide: soyabean oligosaccharides: the functional oligose that Tang oligosaccharide=4:2:1:1:1:1 forms 10 kilograms, mixing and stirring, regulates pH to 6.8 to make slip;
(3) by the slip that step (2) is obtained, insert in fermentation tank, be heated to 100 DEG C and boil 1 hour, then allow it naturally cool to less than 35 DEG C, obtain fermentation medium;
(4) add in the fermentation medium obtained to step (3) every gram of viable bacteria content that embodiment 1 obtains be 20,000,000,000 enterococcus faecalis dry powder 5.5 kilograms, every gram viable bacteria content be 20,000,000,000 addicted to bacterium moth vibrios dry powder 2.75 kilograms and every gram viable bacteria content be 20,000,000,000 5.5 kilograms, saccharomyces cerevisiae dry powder, temperature be 29 DEG C, throughput be 1:0.3V/V/min condition under cultivate 26 hours, obtain fermentation culture;
(5) by fermentation culture obtained for step (4), quantitative separating is in through sterilization treatment and in dried vial, sealing bottleneck packaging, obtains product of the present invention.
Embodiment 5
(1) waste liquid that in being processed by the ginkgo biloba p.e collected, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin Adsorption For Ag flavone, filter through 200 eye mesh screens, vacuum rotary evaporator is adopted to concentrate under temperature 65 DEG C, vacuum 0.065MPa condition, be 1.25 to proportion, obtain ginkgo leaf waste liquid concentrate;
(2) get the ginkgo leaf waste liquid concentrate 100 kilograms that step (1) obtains, add by galactooligosaccharide: the functional oligose that mannan-oligosaccharides=6:4 forms 10 kilograms, mixing and stirring, regulate pH to 6.9 to make slip;
(3) by the slip that step (2) is obtained, insert in fermentation tank, be heated to 100 DEG C and boil 1 hour, then allow it naturally cool to less than 35 DEG C, obtain fermentation medium;
(4) add in the fermentation medium obtained to step (3) every gram of viable bacteria content that embodiment 1 obtains be 20,000,000,000 enterococcus faecalis dry powder 3.5 kilograms, every gram viable bacteria content be 20,000,000,000 addicted to bacterium moth vibrios dry powder 3.5 kilograms and every gram viable bacteria content be 20,000,000,000 5.5 kilograms, saccharomyces cerevisiae dry powder, temperature be 28 DEG C, throughput be 1:0.3V/V/min condition under cultivate 26 hours, obtain fermentation culture;
(5) by fermentation culture obtained for step (4), quantitative separating is in through sterilization treatment and in dried vial, sealing bottleneck packaging, obtains product of the present invention.
Embodiment 6
(1) waste liquid that in being processed by the ginkgo biloba p.e collected, ginkgo leaf leach cooking liquid flows out after macroporous absorbent resin Adsorption For Ag flavone, filter through 200 eye mesh screens, vacuum rotary evaporator is adopted to concentrate under temperature 72 DEG C, vacuum 0.075MPa condition, be 1.25 to proportion, obtain ginkgo leaf waste liquid concentrate;
(2) the ginkgo leaf waste liquid concentrate 100 kilograms that step (1) obtains is got, add by galactooligosaccharide: mannan-oligosaccharides: FOS: the functional oligose that Tang oligosaccharide=3:2:1:1 forms 7 kilograms, mixing and stirring, regulates pH to 6.6 to make slip;
(3) by the slip that step (2) is obtained, insert in fermentation tank, be heated to 100 DEG C and boil 1 hour, then allow it naturally cool to less than 35 DEG C, obtain fermentation medium;
(4) add in the fermentation medium obtained to step (3) every gram of viable bacteria content that embodiment 1 obtains be 20,000,000,000 enterococcus faecalis dry powder 5.5 kilograms, every gram viable bacteria content be 20,000,000,000 addicted to bacterium moth vibrios dry powder 5.5 kilograms and every gram viable bacteria content be 20,000,000,000 2.75 kilograms, saccharomyces cerevisiae dry powder, temperature be 30 DEG C, throughput be 1:0.3V/V/min condition under cultivate 24 hours, obtain fermentation culture;
(5) by fermentation culture obtained for step (4), quantitative separating is in through sterilization treatment and in dried vial, sealing bottleneck packaging, obtains product of the present invention.
Claims (6)
1. a preparation method for liquid feed additive, is characterized in that, is made up of following steps:
(1) collect the waste liquid that flows out after macroporous absorbent resin Adsorption For Ag flavone of ginkgo leaf leach cooking liquid in ginkgo biloba p.e processing, temperature 50-85 DEG C, under vacuum 0.05-0.1MPa condition, being concentrated into proportion is 1.25, obtains ginkgo leaf waste liquid concentrate;
(2) the ginkgo leaf waste liquid concentrate (1) obtained adds compound sugar, regulates pH to 6.6-7.0 to make slip; The wherein addition of compound sugar is 1:0.05-0.1 ratio in the weight ratio of ginkgo leaf waste liquid concentrate and compound sugar;
(3) in the slip impouring fermentation tank (2) obtained, being heated to 100 DEG C and boiling 1 hour, then allow it naturally cool to less than 35 DEG C, is fermentation medium;
(4) fermentation medium (3) obtained adds the enterococcus faecalis of 5000 hundred million-10000 hundred million by fermentation medium weight 1kg, the saccharomyces cerevisiae eating into vibrios and 5000 hundred million-10000 hundred million addicted to bacterium of 5000 hundred million-10000 hundred million, under 27-30 DEG C of condition, ventilate cavity 24-36 hour, obtains fermentation culture;
(5) by fermentation culture quantitative separating obtained to (4) in through sterilization treatment and in dried vial or plastic bottle, sealing bottleneck, packs to obtain product.
2. the preparation method of liquid feed additive according to claim 1, is characterized in that, described step (1) preferably concentrated condition is 60 DEG C-75 DEG C, 0.06-0.08MPa; Preferred use vacuum rotary evaporator or plate evaporation inspissator.
3. the preparation method of liquid feed additive according to claim 1, is characterized in that, the compound sugar described in described step (2) is 2-10 monose; Described compound sugar is specially galactooligosaccharide, mannan-oligosaccharides, xylo-oligosaccharide, FOS, chitosan oligomer, malto-oligosaccharide, soyabean oligosaccharides, Tang oligosaccharide.
4. the preparation method of liquid feed additive according to claim 3, it is characterized in that, the mass ratio of described galactooligosaccharide, mannan-oligosaccharides, xylo-oligosaccharide, FOS, chitosan oligomer, malto-oligosaccharide, soyabean oligosaccharides, Tang oligosaccharide is 3-6:0-4:0-3:0-2:0-2:0-3:0-2:0-2.
5. the preparation method of liquid feed additive according to claim 1, is characterized in that, the condition of the ventilate cavity described in described step (4) is throughput is 1:0.3V/V/min.
6. the liquid feed additive product obtained as any one of claim 1-5 preparation method.
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| CN1504102A (en) * | 2002-11-29 | 2004-06-16 | 上海九川科技发展有限公司 | Process of fodder with bioactivity |
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| CN101627796A (en) * | 2009-08-13 | 2010-01-20 | 宜春强微生物科技有限公司 | Liquid feed additive and preparation method thereof |
| CN101912056A (en) * | 2010-07-16 | 2010-12-15 | 张留医 | Method for preparing drug residue free feed for feeding live pigs |
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| CN103005159A (en) * | 2012-12-18 | 2013-04-03 | 南京林业大学 | Preparation method of ginkgo leaf biological feed additive |
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