CN104491241A - Persimmon tannin aloe compound cream with ionizing radiation resistance - Google Patents

Abstract

The invention discloses persimmon tannin aloe compound cream with ionizing radiation resistance. The compound cream comprises the following components in parts by mass: 2-5 parts of persimmon powder, 2-4 parts of honeysuckle extract, 1-3 parts of wolfberry extract, 5-8 parts of primary color whole leaf aloe freeze-dried powder, 2-5 parts of tea polyphenol, 2-5 parts of glycerin and the balance of jelly for medical purposes. The content of persimmon tannin in the persimmon powder is 2-10 percent; and the content of active ingredients in the persimmon tannin aloe compound cream is that in every 100g of the product, the content of persimmon tannin is 150-250mg, and the content of O-acetyl is more than 600mg. The product is subjected to an ionizing radiation-resistant experiment, the cell activity is detected by adopting a CCK-8 method, the 293T cells are taken as research objects, the total dose during radiation is 8Gy, and the total culture time is 12 hours after radiation. The cellular morphology observation proves that the persimmon tannin aloe compound cream has the most obvious ionizing radiation resistance when the concentration of the persimmon tannin is 2mg/ml.

Description

A kind of persimmon tannin Aloe compound recipe unguentum with ionizing radiation-resistant effect

Technical field

The present invention relates to medicines and health protection field, especially a kind of externally used paste product with ionizing radiation-resistant effect.

Background technology

Astringent persimmon fruit has another name called Zhu fruit, is the fruit that a kind of nutritive value is very high, enjoys the good reputation of " fruit Zisco product ".Containing about 2.0% puckery persimmon tannin in astringent persimmon fruit, also containing aminoacid, trace element, multivitamin, organic acid etc. that saccharide, protein, human body are necessary, have and suppress the multiple efficacies such as human lymphocyte leukemia, blood pressure lowering, removing interior free yl, absorption yttrium.Application for a patent for invention CN101328194 discloses a kind of persimmon powder and production technology thereof; It is after being squeezed the juice by Fructus Kaki, and juice carries out enzymolysis, filtration, enzyme denaturing, concentrated, vacuum lyophilization and obtained tannin content is the powder of 1% ~ 99%.

Fructus Lycii is the fruit of plant of Solanaceae lycium barbarum Lyci μm of barbar μm of L..China's tradition rare Chinese medicine, sweet, the micro-acid of feeble QI, taste.Main component is lycium barbarum polysaccharide, betanin, zeaxanthine, carotene and aminoacid etc.There is the effects such as nourishing the liver and kidney, replenishing vital essence to improve eyesight, immunomodulating, hemopoietic, also have defying age, mutation, antitumor, blood fat reducing, protect the liver, the effect such as blood sugar lowering, blood pressure lowering.In commercially available wolfberry fruit extract, the content of lycium barbarum polysaccharide is about 50%.

Flos Lonicerae, has another name called Radix Ophiopogonis, its effect mainly heat-clearing and toxic substances removing, cures mainly epidemic febrile disease heating, toxic-heat and blood stasis, carbuncle furunculosis etc.Flos Lonicerae contains the pharmacological component such as chlorogenic acid, luteolin glycosides, stronger restraint is had to the various pathogens such as Hemolytic streptococcus, staphylococcus aureus and upper respiratory tract infection Causative virus etc., in addition also can enhancing immunity, hepatoprotective, antitumor, antiinflammatory, antipyretic etc.

Tea polyphenols is the All Pure Nature antioxidant food extracted from Folium Camelliae sinensis, refer to the Polyphenols that tea Ye Zhongyi large class composition complexity, molecular weight and architectural difference thereof are very large and derivant mixture thereof, primarily of the organic compound that catechin, flavonol, anthocyanidin, phenolic acid and depside thereof etc. form, based on catechin.Tea polyphenols can extremely strong removing harmful free radicals, blocks lipid peroxidation process, improves the activity of human enzymes, thus play effect of mutation, anticancer, to the prevention of the kinds cancer such as gastric cancer, intestinal cancer and auxiliary treatment all helpful.

Aloe contains the functional components such as anthraquinone analog compound, Aloe polysaccharide, aminoacid, organic acid, vitamin, mineral, enzyme, can be used for food, medicine, cosmetics, has health-care effecies such as improving immunity, fat-reducing, beauty treatment.Industry standard QB/T2489-2007 defines production technology and the quality index thereof of primary colors aloe leaf lyophilized powder, wherein defines Aloe polysaccharide content, in O-acetyl content, remains the active component of Aloe.

Electromagnetic radiation is a kind of important energy pollution, mixes by direct or indirect ionizing particle or the two radiation formed.Ionizing radiation is the radiation general name that all can cause material to ionize, and its kind is a lot, the ray that mainly wavelength is short, frequency is high, energy is high.Electromagnetic radiation phenomenon not can occur only on the mobile communication equipment such as mobile phone, pager of people's concern, also comprises radio and television, communication, medical treatment, traffic, power transmission etc.Such as, radio and television launching tower, household microwave oven, Dimmable desk lamp etc. all can release electromagnetic wave, and just power varies.Ionizing radiation can cause radiation sickness, can be divided into acute radiation injury and chronic radioactive damage to the damage of body.Accept heavy dose of irradiation in short time, the acute injury of body can be caused, be common in nuclear accident and radiation therapy patient.And dispersion accepts the irradiation of doses in the long period, chronic radioactive can be caused to damage, as skin injury, hematopoietic disorders, leukopenia, impaired fertility etc.In addition, radiation can also carcinogenic death and deformity with causing fetus.The externally applied product that exploitation has ionizing radiation-resistant is trend of the times.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of persimmon tannin Aloe compound recipe unguentum with ionizing radiation-resistant effect.

In order to solve this technical problem, the present invention adopts persimmon tannin content to be the persimmon powder of 2-10%, wolfberry fruit extract, meet primary colors aloe leaf lyophilized powder that light industry standard QB/T2489-2007 specifies, Flos Lonicerae extract, tea polyphenols be primary raw material, add glycerol and medical ventolin, make compound recipe unguentum.

Have a persimmon tannin Aloe compound recipe unguentum for ionizing radiation-resistant effect, formula consists of, by mass%:

Persimmon powder 2-5;

Flos Lonicerae extract 2-4;

Wolfberry fruit extract 1-3;

Primary colors aloe leaf lyophilized powder 5-8;

Tea polyphenols 2-5;

Glycerol 2-5;

Surplus is medical ventolin.

In finished product, functional component content is: in every 100g product, persimmon tannin content is that 150-250mg, O-acetyl group is greater than 600mg.

In described persimmon powder, persimmon tannin content is 2-10%;

Described Flos Lonicerae extract, wolfberry fruit extract and tea polyphenols are commercially bought;

Described primary colors aloe leaf lyophilized powder meets light industry standard QB/T2489-2007 to specify.

The preparation method of this unguentum is:

By each middle plant extract raw material pulverizing, mixing, then adds glycerol, adds medical ventolin, be deployed into ointment preparation, must have the persimmon tannin compound recipe unguentum of ionizing radiation-resistant effect.

When this product uses, spread upon on the face or corresponding skin surface, and soft a moment.

This product is done ionizing radiation-resistant experiment, adopt CCK-8 method to detect cytoactive.

Because the sensitivity of 293T to X-ray is high; select 293T cell as object of study; using persimmon tannin compound recipe unguentum as test medicine, verify that persimmon tannin compound recipe unguentum is to the protective effect of radiation damage by cellular morphology observation, CCK-8 detection cell viability, Hoechst33258 staining examine apoptosis, flow cytomery apoptosis.Experiment shows, along with the increase of radiation dose, the activity of cell reduces gradually.Be 8Gy at total radiation dose, 12h is cultivated after radiation, observed by cellular morphology and show, the persimmon tannin unguentum adding variable concentrations before irradiation all has certain protective action to cell, but the concentration of persimmon tannin has the most obvious protective action when 2mg/ml.

The present invention has following advantages and characteristic:

1, quality better.With O-acetyl group in the persimmon tannin in persimmon powder and Aloe powder for main active, adopt persimmon powder, wolfberry fruit extract, primary colors aloe leaf lyophilized powder; Flos Lonicerae extract; tea polyphenols carries out compound recipe, makes externally used paste, has obvious radiation resistance.Especially when total radiation dose is 8Gy, controlling functional component content in plaster products is: in every 100g product, and persimmon tannin content is 150-250mg, O-acetyl group when being greater than 600mg, has good ionizing radiation-resistant effect.Persimmon tannin content is 200mg, O-acetyl group when being greater than 600mg, ionizing radiation-resistant best results.Composite according to formula, concentrate various active substance elite, quality better.

2, various effective ingredient, is supported mutually together, cooperates with each other, and serves potentiation; Combined effect highlights the ionizing radiation-resistant effect of persimmon tannin, and side effect is low.

Detailed description of the invention

The following stated example understands the present invention in detail.In instances, unless otherwise indicated, all numbers and percentages being by weight.

Embodiment 1

1, according to formula, satisfactory various extract raw material super micron mill is pulverized, for subsequent use.

2, taking persimmon tannin content is respectively 5.0% persimmon powder 400g; Flos Lonicerae extract 400g; Wolfberry fruit extract 200g; Primary colors aloe leaf lyophilized powder 600g; Tea polyphenols 300g; Glycerol 400g; Medical ventolin 7700g.

3, the persimmon powder 400g weighed up, Flos Lonicerae extract 400g, wolfberry fruit extract 200g, primary colors aloe leaf lyophilized powder 600g, tea polyphenols 300g are added in homogenizer, add glycerol 400g, then medical ventolin 7700g is added, carry out stirring, homogenizing, make paste, the persimmon tannin compound recipe unguentum of ionizing radiation-resistant effect must be had.After testing, in every 100g product, persimmon tannin content is that 200mg, O-acetyl group is greater than 800mg.

4, CCK-8 method is adopted to detect cytoactive

(1) choose the 293T cell of exponential phase, make 1 × 10 ⁴the single cell suspension of individual/ml, then seed cells in 96 orifice plates, each hole adds the DMEM fresh medium of 100 μ l containing 10% new-born calf serum, and the edge of 96 orifice plates is filled with aseptic PBS, then constant-temperature enclosed cell culture incubator (temperature: 37 DEG C, CO is placed in ₂content: be cultured to cell monolayer 5%) and be paved with at the bottom of hole.

(2) cell culture 24h is to after adherent, removes old culture fluid, then adds the DMEM fresh medium of 100 μ l containing 10% new-born calf serum, cultivates 12h, make 293T cell synchronization.

(3) after cell synchronization, old culture fluid is abandoned.Every hole adds DMEM culture fluid and the 10ml medicinal liquid that 90ml contains l0% new-born calf serum.Medicine group sets up 6 concentration subgroups (0 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 400 μ g/ml, 800 μ g/ml) separately by the final concentration of every hole tannin, arrange blank group simultaneously, blank group is acellular culture medium, every subgroup establish 4 parallel, cultivate after 24 hours, the X-ray (4GY, 8GY, 12GY, 16GY, 20GY) that 293T cell accepts various dose is irradiated.Arrange matched group, matched group does not receive the irradiation of X-ray simultaneously.

(4) after radiation, after 12h, 24, by the old liquid reject in 96 well culture plates, wash 3 times by aseptic PBS solution, then every hole adds the fresh DMEM culture medium of 90 μ l and 10ml CCK-8 liquid continuation cultivation 4h respectively.

(5) after 4h, 96 porocyte culture plates are placed in absorbance (OD) value that (490nm, 1S) under multi-functional microplate reader measures each hole.

(6) calculate cell viability, the results are shown in Table 1, table 2.

The cell viability value (%) of the different radiation dose of 12h and different persimmon tannin drug level after table 1. radiation

The cell viability value (%) of the different radiation dose of 24h and different persimmon tannin drug level after table 2. radiation

Visible, be 8Gy at total radiation dose, after radiation, cultivate 12h, observed by cellular morphology and show, the persimmon tannin unguentum adding variable concentrations before irradiation all has certain protective action to cell, and the concentration of persimmon tannin has the most obvious ionizing radiation-resistant effect when 2mg/ml.

Embodiment 2

1, according to formula, satisfactory various extract raw material super micron mill is pulverized, for subsequent use.

2, taking persimmon tannin content is respectively 2.0% persimmon powder 1000g; Flos Lonicerae extract 200g; Wolfberry fruit extract 100g; Primary colors aloe leaf lyophilized powder 500g; Tea polyphenols 500g; Glycerol 500g; Medical ventolin 7200g.

3, the persimmon powder weighed up, Flos Lonicerae extract, wolfberry fruit extract, primary colors aloe leaf lyophilized powder, tea polyphenols are added in homogenizer, add glycerol, then medical ventolin is added, carry out stirring, homogenizing, make paste, the persimmon tannin compound recipe unguentum of ionizing radiation-resistant effect must be had.After testing, in every 100g product, persimmon tannin content is that 248mg, O-acetyl group is greater than 700mg.

Embodiment 3

1, according to formula, satisfactory various extract raw material super micron mill is pulverized, for subsequent use.

2, taking persimmon tannin content is respectively 8.0% persimmon powder 250g; Flos Lonicerae extract 300g; Wolfberry fruit extract 300g; Primary colors aloe leaf lyophilized powder 800g; Tea polyphenols 300g; Glycerol 200g; Medical ventolin 7950g.

3, the persimmon powder weighed up, Flos Lonicerae extract, wolfberry fruit extract, primary colors aloe leaf lyophilized powder, tea polyphenols are added in homogenizer, add glycerol, then medical ventolin is added, carry out stirring, homogenizing, make paste, the persimmon tannin compound recipe unguentum of ionizing radiation-resistant effect must be had.After testing, in every 100g product, persimmon tannin content is that 158mg, O-acetyl group is greater than 900mg.

Claims (1)

1. have a persimmon tannin Aloe compound recipe unguentum for ionizing radiation-resistant effect, it is characterized in that: by mass%, its formula is:

Persimmon powder 2-5;

Flos Lonicerae extract 2-4;

Wolfberry fruit extract 1-3;

Primary colors aloe leaf lyophilized powder 5-8;

Tea polyphenols 2-5;

Glycerol 2-5;

Surplus is medical ventolin;

In described persimmon powder, persimmon tannin content is 2-10%;

In every 100g persimmon tannin compound recipe plaster products, persimmon tannin content is that 150-250mg, O-acetyl group is greater than 600mg.