CN104489646A - Fruit and vegetable probiotic tablet and preparation method thereof - Google Patents

Fruit and vegetable probiotic tablet and preparation method thereof Download PDF

Info

Publication number
CN104489646A
CN104489646A CN201410658354.5A CN201410658354A CN104489646A CN 104489646 A CN104489646 A CN 104489646A CN 201410658354 A CN201410658354 A CN 201410658354A CN 104489646 A CN104489646 A CN 104489646A
Authority
CN
China
Prior art keywords
parts
powder
probiotic
weight
vegetables
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410658354.5A
Other languages
Chinese (zh)
Other versions
CN104489646B (en
Inventor
李雅君
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Xuyou Food Technology Co.,Ltd.
Original Assignee
李雅君
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 李雅君 filed Critical 李雅君
Priority to CN201410658354.5A priority Critical patent/CN104489646B/en
Publication of CN104489646A publication Critical patent/CN104489646A/en
Application granted granted Critical
Publication of CN104489646B publication Critical patent/CN104489646B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/125—Casei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/21—Streptococcus, lactococcus
    • A23V2400/249—Thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/517—Bifidum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/519—Breve
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/529—Infantis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

Description

A kind of fruits and vegetables probiotic tablet and preparation method thereof
Technical field
The present invention relates to probiotic tablet, be specifically related to a kind of fruits and vegetables probiotic tablet with antiobesity action and preparation method thereof.
Background technology
Probio (Probiotics), also known as probiotic, regulator, raw rhzomorph, viable bacteria element etc., namely certain microorganism formulation or fermented product is referred to, by regulating host's mucous membrane and systemic immune function and improving enteral nutrition and colony balance, useful physiological action is produced to host, changed the composition of host a certain position flora by field planting effect, thus generation is conducive to the single of host health effect or forms clear and definite mixed microorganism.Up to now, the probio that scientist has found can be divided into three major types substantially, comprising: 1. lactobacillus class (as lactobacillus acidophilus, Lactobacillus casei, lactobacillus bulgaricus, lactobacillus plantarum etc.); 2. Bifidobacterium class (as bifidobacterium longum, bifidobacterium adolescentis, bifidobacterium breve, bifidobacterium infantis etc.); 3. gram-positive cocci (as streptococcus fecalis, galactococcus, intermediary streptococcus etc.).As a frontier, in recent years, the special physiological activity of probio is widely used in food, except being applied to fermented dairy product, is also just progressively applied to developing various functional food.At present, probio is mainly used in the fields such as food, functional food, dietary supplements, medicinal organism goods and feed.Food containing profitable probliotics is referred to as probiotic food, mainly comprises and directly add the food of probio and the food through probiotics fermention.The application the most general in field of food of probio is still dairy products field, comprises acidified milk, active lactic acid bacteria drink, cheese, sour cream, kefyr, fruit juice, cereal preparation, biscuit etc.Probio bacterial classification have Bifidobacterium (Bifidobateria), Bacillus acidi lactici and some streptococcus, wherein, what Bacillus acidi lactici was conventional has lactobacillus acidophilus (Lactobicullus acidophilus), Lactobacillus casei (L.casei), lactobacillus bulgaricus (L.bulgaricus) etc.
At present, research about probiotic tablet is more: Chinese patent CN 101700253B discloses a kind of probiotic tablet and preparation method thereof, with glucose, lactose, microcrystalline cellulose, the auxiliary materials such as skimmed milk power are that particle prepared by raw material, mixed active composition probio powder and dolomol carry out compressing tablet again, finally obtain the production method of probiotic tablet and apply the high probiotic tablet of a kind of Viable detection that this kind of method produce, advantage is: the probiotic tablet of preparation because of its Viable detection high, taking convenience, be convenient to absorb, result for the treatment of is obvious, and it is low that non-slurry pelletizing technology provided by the invention has cost, discharging speed is fast, stable process conditions, favorable reproducibility, probio survival rate advantages of higher, be applicable to large-scale production.Chinese patent CN 103876145A discloses a kind of probiotics micro-ecological tablet and preparation method thereof, belongs to the technical field of health food and processing.Probiotics micro-ecological tablet is probiotics bacterial 90% ~ 98% by mass, FOS 1% ~ 9%, dolomol 0.1% ~ 1%.Preparation is activated by Bifidobacterium, and prepare Kefir fermenting agent; Again the two is obtained through mixed culture fermentation low temperature concentrate drying, batch mixing compressing tablet.Mixed culture fermentation is inoculated by probio mixed bacteria, 28 ~ 36 DEG C of condition bottom fermentations 16 ~ 24 hours, the ratio 1 ~ 3:1 of Kefir fermenting agent and Bifidobacterium in mixed bacteria.Kefir fermenting agent and Bifidobacterium are carried out high density mixed culture fermentation by the present invention, and bacterial classification is various, stable storing, and the viable count in tablet reaches 2,000,000,000 more than cfu/g respectively; Not only can improve digestion power, also enhance the colonization ability of probio in enteron aisle simultaneously; Have and be of high nutritive value, the abilities such as unique flavor.Application publication number is that the Chinese patent of CN 102348388A discloses a kind of stable probiotic granulate and preparation method thereof, relates to the probiotic composition field for people and/or animal foodstuff and health products.The invention particularly relates to the method for granulating relevant to the composition comprising probiotic micro-organisms, for the production of the composition containing stable probiotic granulate, described probiotic granulate keeps stable during producing the tablet or particle being used for final use.Present invention applicant working result display, during granulation the death rate of probiotic micro-organisms and the characteristic of granulation procedure condition and selected microorganism closely related.Described work establishes the mathematic(al) representation for limiting yeast character and method parameter, makes microorganism loss after granulating be less than the 1log CFU/g of granular food.Chinese patent CN 10155908A discloses the preparation method of the probiotics preparation of a kind of reducing blood lipid and regulating intestinal canal flora, and Kefir lactobacillu plantarurn MA2 is carried out High Density Cultivation for (1) by its step, and obtain zymotic fluid, its cell density is not less than 10 8cfu/mL; (2) above-mentioned fermentation liquor collected by centrifugation obtains thalline, adds protective agent, is lyophilized into bacterium powder; (3) in the bacterium powder of freeze-drying, add oligosaccharide mixture, mix and obtain probiotics preparation finished product.Also there is while these product have hypolipemic function the physiological function of regulating intestinal canal flora, can treat and prevent hyperlipidemic conditions and regulating intestinal canal flora, and nontoxic, safety, long shelf-life, can be made into capsule, microcapsules, electuary, tablet etc.
Above-mentioned disclosed patent mainly concentrates on to increase probio kind, improve tablet viable bacteria content, Cardiovarscular, beautifying face and moistering lotion, improve in product for the purpose of stability etc. and preparation method, although have their own characteristics each, but the microcrystalline cellulose used is mainly with timber, cotton, cotton linter, wheat straw, straw, reed, fiber crops, mulberry skin, paper mulberry skin and bagasse etc. are that raw material is through slurrying, bleaching, mechanical dispersion, pulverizing waits Industrialized synthesis method preparation, there is larger food security hidden danger, the probiotic products research simultaneously with weight losing function is less, at present, fat more and more significant on the impact of human health, therefore genesis mechanism and the therapeutic strategy of studying obesity are very necessary.Research shows, gut flora can help host to obtain more calorie from the polysaccharide component diet, and is stored in adipocyte for subsequent use.Gut flora can also increase the storage of fat in adipose tissue and liver by the expression of closing Fiaf gene and increase ChREBP/SREBP, Acc1 and Fas.Further, the lipopolysaccharides LPS that in enteron aisle, Gram-negative bacteria produces, has caused the reaction of whole body chronic low-level inflammation, has then causeed fat.Therefore, be expected to by probio, prebiotics or antibiotics modification gut flora the effective ways becoming future therapeutic obesity.
Summary of the invention
Technical problem solved by the invention is the deficiency overcoming existing probiotic tablet, with probio pulvis such as Lactobacillus plantarums for primary raw material, the composite modified dietary fiber of science, fruit vegetable powder, compound sugar, plant extracts, albumen powder, tea extract, Chinese herbal medicine extract etc., improve the content of soluble cellulose beneficial flora to real meaning, enhance cellulosic physiologically active, and then while increase beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and that external source probio is in human body intestinal canal colonization ability and resident time, effectively inhibit the harmful intestinal tract bacteria especially growth of Gram-negative bacteria and breeding, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, prepare a kind of biologically active high, long at human body intestinal canal resident time, fat-reducing effect significantly and suitable population's fruits and vegetables probiotic tablet widely.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight:
Probio pulvis 60-80 part, modified dietary fiber 35-45 part, fruit vegetable powder 25-35 part, compound sugar 20-30 part, plant extracts 25-30 part, albumen powder 18-25 part, tea extract 15-20 part, Chinese herbal medicine extract 8-10 part, sweetener 2-5 part, dolomol 0.2-0.5 part;
Described probio pulvis probiotics viable bacteria content is: 7x10 12-9x10 12cfu/g;
Further, described probio pulvis include but not limited in following probio pulvis one or more: such as: Lactobacillus plantarum (Lactobacillus plantarum), bifidobacterium bifidum (Bifidobacterium bifidum), bifidobacterium infantis (B.infantis), long Bifidobacterium Bifidum (B.longum), short Bifidobacterium Bifidum (B.breve), bifidobacterium adolescentis (B.adolescentis), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), lactobacillus acidophilus (L.acidophilus), Lactobacillus casei (Lactobacillus casei), streptococcus thermophilus (Streptococcus thermophilus), also comprise for one or more in the fungi pulvis of health food, such as: saccharomyces cerevisiae (Saccharomyces cerevisiae), candida utili (Cadida atilis), Kluyveromyces lactis (Kluyveromyces lactis), saccharomyces carlsbergensis (Saccharomyces carlsbergensis), Paecilomyces hepiali chen (Paecilomyces hepiali Chen et Dai, sp.nov), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen), glossy ganoderma (Ganoderma lucidum), purple sesame (Ganoderma sinensis), Ganoderma tsugae (Ganoderma tsugae), monascus (Monacus anka), monascus purpureus (Monacus purpureus),
Preferably, described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 45-55 part, bifidobacterium longum 35-45 part, bifidobacterium bifidum 25-35 part, bifidobacterium infantis 25-35 part, bifidobacterium adolescentis 15-20 part, lactobacillus bulgaricus 15-20 part, lactobacillus acidophilus 10-15 part, Lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 5-10 part, candida utili bacterium 4-6 part;
Preferably, described Lactobacillus plantarum pulvis is prepared by Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City. Institute of Microorganism, Academia Sinica. and postcode: 100101), deposit number is CGMCC NO.9405, and Classification And Nomenclature is: Lactobacillus plantarum Lactobacillus plantarum.
Further, dietary fiber obtains through physics, chemistry or biological method process by described modified dietary fiber, Soluble Fiber cellulose content is high, biologically active strong, have cellulose that is important, positive role to beneficial bacteria of intestinal tract group, compared with full diet fiber, its biological action is more powerful: 1) Soluble Fiber cellulose content is high, more easily utilized by probio, probio can be improved in the growth of enteron aisle and fertility, increase kind and the quantity of probio flora, reduce large intestine pH value, improve intestine microenvironment; 2) powerful adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; 3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger; 4) the stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property;
Preferably, described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber, rice bran, wheat bran;
More preferably, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber, rice bran, wheat bran 7-12:6-9:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, simultaneously in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 6-8:4-7:3-5:2-4:1-3:1-3:0.3-0.5 Homogeneous phase mixing in mass ratio.
Further, described fruit vegetable powder is one or more Homogeneous phase mixing in acerola concentrate powder, lemon powder, leek powder, cucumber powder, white radish powder, white gourd powder, mung bean sprouts powder, strawberry powder, carrot meal, celery powder, papaya powder, pineapple powder, orange powder, oranges and tangerines powder, sweet orange powder, coconut slurry powder, Cranberry powder, blueberry powder;
Preferably, the mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 13%, leek powder 10%, celery powder 7%, white gourd powder 7%, strawberry powder 5%, carrot meal 5%, papaya powder 5%, pineapple powder 5%, mung bean sprouts powder 5%, orange powder 3%, oranges and tangerines powder 3%, sweet orange powder 3%, coconut slurry powder 4%, Cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blueberry powder 2%;
Preferably, the preparation method of each fruit vegetable powder described is: fruits and vegetables are put into the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer and carry out microwave drying 6-12min under power 3000W, frequency 2000Hz, temperature 50-60 DEG C condition, until moisture is down to 5-8%, then in 20-30 DEG C, be crushed to granularity 0.1-0.3mm, pack and obtain fruit vegetable powder.
Further, described compound sugar is at least one in FOS, stachyose, gossypose, xylo-oligosaccharide, galactooligosaccharide, soyabean oligosaccharides, oligoisomaltose, oligomeric maltose;
Preferably, described compound sugar parts by weight consist of FOS 40-50 part, stachyose 30-40 part, gossypose 20-40 part, xylo-oligosaccharide 16-18 part, galactooligosaccharide 10-15 part, soyabean oligosaccharides 10-15 part, oligoisomaltose 10-15 part, oligomeric maltose 8-12 part.
Further, described plant extracts is significantly can promote lactobacter growth, increases the natural plants of viable lactic acid bacteria content and the higher raw-food material of leptin content extracts through science and obtains;
Preferably, the preparation method of described plant extracts is: count by weight, accurately take jerusalem artichoke 30-50 part, Chinese yam 30-40 part, balsam pear 20-40 part, almond 20-40 part, burdock 20-30 part, lotus seeds 15-25 part, cassia seed 15-20 part, onion 10-15 part, asparagus 5-10 part, being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of mixture 5 times of weight in container, control temperature 80 DEG C keeps 3h, then 50 DEG C are cooled to, the mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by apple vinegar adjust ph, enzymolysis 3h, finally add the mixture of mixed material 1.8 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filter, filtrate reduced in volume, freeze drying, low-temperature grinding is to granularity 0.1-0.3mm, then the vitamin D of crushed material quality 0.01-0.03% is added, mix and obtain plant extracts,
The parts by weight of described mixed enzyme consist of: zytase 18 parts, pentosanase 18 parts, dextranase 15 parts, 15 parts, lipase, Pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts;
Described concentration of alcohol is 75%.
Further, described albumen powder is one or more in sour milk powder, soybean protein, casein, lactalbumin;
Preferably, described albumen powder percentage by weight consists of: sour milk powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%;
Described sour milk powder is commercial commodity, and viable lactic acid bacteria content is 3x10 11-5x10 11cfu/g.
Further, the preparation method of described tea extract in turn includes the following steps: commercially available tealeaves is put into the supersonic wave cleaning machine that 0.8% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, then in the sterile malt dextrin in aqueous solution of 0.25-0.35MPa pressure lower surface even application mass percent concentration 0.5-1.5%, the mass ratio of tealeaves and the maltodextrin aqueous solution is: 100:0.5-15, sealing, rehydration 2-8h under room temperature; Then in-21--25 DEG C of freezing 10-30min; Pulverize immediately, granularity is 0.1-2mm; Tealeaves after pulverizing is placed in pill tank, adds tea quality 5-10 sterilized water doubly, room temperature 200W, 40KHz condition ultrasonic extraction 10-15min; Simultaneously in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be finally 4.5-6.5 by said extracted liquid lactic acid adjust ph, add the complex enzyme of mixture quality 0.5-1.5%, in 35-45 DEG C of low temperature enzymolysis 4-12h; Enzymolysis liquid filtration, filtrate reduced in volume, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains tea extract;
Described complex enzyme is one or more in pectase, cellulase, tannase, protease;
Preferably, the mass ratio of described pectase, cellulase, tannase and protease mixing is 1-2:2-4:2-4:1-3.
Further, the preparation method of described Chinese herbal medicine extract is: count by weight, accurately takes tarragon 50-55 part, garlic 50-55 part, Herba portulacae 45-55 part, cordate houttuynia 45-55 part, root of large-flowered skullcap 30-45 part, golden cypress 30-40 part, hawthorn 30-40 part, Radix Glycyrrhizae 25-35 part, kuh-seng 15-25 part, rhizoma alismatis 8-10 part, giant knotweed 6-8 part, rheum officinale 2-5 part, semen brassicae 2-5 part, tuber of multiflower knotweed 1-3 part; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of mixture 5 times of weight in container, control temperature 80 DEG C keeps 3h, is then cooled to 50 DEG C, the compound enzyme adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally adds the mixture of mixed material 1.8 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2.5:1.5, reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains Chinese herbal medicine extract;
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase 12 parts, zytase 18 parts, pentosanase 18 parts, Pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts, 6 parts, lipase;
Described concentration of alcohol is 75%.
Further, described sweetener is that one or more in antierythrite, xylitol, maltitol, D-sorbite, stevioside, mogroside, Aspartame mix with arbitrary proportion.
The preparation method of described fruits and vegetables probiotic tablet, comprise the steps: accurately to take each component raw material by above-mentioned formula, first by probio pulvis and modified dietary fiber Homogeneous phase mixing 20-30min, then Chinese herbal medicine extract is added successively, tea extract, albumen powder, plant extracts, compound sugar, fruit vegetable powder Homogeneous phase mixing 10-15min, compound is added dry granulating machine granulation, collect 10-100 object particle, add sweetener successively, the raw material of directly compressible is obtained after dolomol mixing, drop into tabletting machine, adjustment pressure is 10-40kn, obtain fruits and vegetables probiotic tablet.
In above-mentioned preparation method, fruits and vegetables probio can be pressed into diameter 6 ~ 12mm, sheet weighs 0.3 ~ 0.5g, hardness 8 ~ 10kg/cm 2tablet.
Described fruits and vegetables probiotic tablet probiotics viable bacteria content is: 6x10 11-8x10 11cfu/g.
Fruits and vegetables probiotic tablet of the present invention can be used for daily supplementary human body probio, also can be used as obesity patient and to lose weight accesary foods, can also use as raw-food material.
Another object of the present invention is the application of described fruits and vegetables probiotic tablet in fat-reducing.
Operation instruction:
1. product specification: 32 x 0.4g;
2. daily probio is supplemented: warm water is oral, a 0.4-0.8 gram, 2 times on the one.
3. obesity patient: warm boiled water, be grown up a 0.8-2 gram, three times on the one.
4. suitable population: not yet find to be not suitable for crowd.
Lactobacillus plantarum of the present invention (Lactobacillus plantarum) tlj-2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute. Institute of Microorganism, Academia Sinica. and postcode: 100101), deposit number is CGMCC NO.9405, and Classification And Nomenclature is: Lactobacillus plantarum Lactobacillus plantarum.
Described lactobacillus plantarum strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram's staining is positive, and atrichia does not produce gemma; On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indoles experiment (+), motility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), produce hydrogen sulfide gas (-), in pH4.0MRS culture medium, grow (+).
Described Lactobacillus plantarum adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.
Described starting strain is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, almost stops growing when medium pH is 3.5, is 0.34mg/h/kg Chinese cabbage to the decomposition rate of natrium nitrosum.Starting strain is the greenfeed that Li Zheng is collected in Fattening Sheep field, Yanchi county Ningxia, acquisition time on September 15th, 2013.
In order to improve the decomposition rate of its production of lactic acid speed, acid-fast ability and nitrite, DES and NTG technology is adopted to carry out mutagenesis to this bacterial classification successively, after mutagenesis, bacterial strain adopts MRS calcium carbonate flat board to carry out primary dcreening operation, then 500mL shake flask fermentation is adopted, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that seed selection is excellent, then does passage assays, evaluates its genetic stability.
Lactobacillus plantarum tlj-2014 genetic stability result shows: through continuous passage ten times, property indices is all more stable, and heredity is better, and proterties is not replied, therefore using the object bacterial strain that Lactobacillus plantarum tlj-2014 obtains as seed selection.
Empirical tests finds: the production of lactic acid speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
Therefore adopt this bacterial classification produce pickles, whole sweat nitrite concentration at below 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
Beneficial effect:
Fruits and vegetables probiotic tablet of the present invention with probio pulvis such as Lactobacillus plantarums for primary raw material, the composite modified dietary fiber of science, fruit vegetable powder, compound sugar, plant extracts, albumen powder, tea extract, Chinese herbal medicine extract etc., improve the content of soluble cellulose beneficial flora to real meaning, enhance cellulosic physiologically active, and then while increase beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and that external source probio is in human body intestinal canal colonization ability and resident time, effectively inhibit the harmful intestinal tract bacteria especially growth of Gram-negative bacteria and breeding, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, the fruits and vegetables probiotic tablet biologically active of preparation is high, long at human body intestinal canal resident time, fat-reducing effect is remarkable and suitable population is extensive.The powerful network of modified dietary fiber especially of the present invention and absorption affinity, by probiotic powder parcel wherein, add the bacteriostasis of Chinese herbal medicine extract, stability that the antioxidation etc. of tea extract enhances fruits and vegetables probiotic tablet, improve the probiotics viable bacteria survival rate of tablet, airtight preservation at the temperature of 25 DEG C, place that conventionally to detect viable count in tablet and calculate survival rate after 12 months be 83-89%, improve 24-30% than existing probiotic tablet Viable detection; Shown by heat resistant test, SGF and intestinal juice resistance test, simulation cholate resistance test, the probio in tablet can keep higher Viable detection, and its Viable detection can reach 88-99%, significantly probiotic; Show through mouse intestinal performance test: probiotic tablet treatment group Mouse Weight average growth rate (53.31%) is significantly higher than natural recovering group (32.14%); Sheet agent solution rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 80.89%, significantly lower than natural recovering group (24.78%), show the probio rapid field planting in small white mouse enteron aisle in fruits and vegetables probiotic tablet of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogens such as Escherichia coli, and resident time is long, continues, effectively improve enteron aisle performance; Suitable population is extensive, fat-reducing effect is good, and after taking one-period (30 days), the number that body weight reduces more than 1-10kg is 95 people, fat-reducing effect reaches more than 95%, and wherein body weight reduction 1-5kg, 5-10kg, more than 10kg are respectively 53%, 26% and 16%; The more significant age bracket of fat-reducing effect be 25-50 year more than, wherein body weight reduce the crowd of more than 10kg be mainly 25-30 year, be secondly 30-50 year crowd; The population of weight of more than 50 years old reduces main at below 10kg, visible, and the overweight people that fruits and vegetables probiotic tablet of the present invention can be applicable to various all ages and classes eats, and has significant fat-reducing effect; Meanwhile, the severe obesity person reducing more than 10kg accounts for 37.5%, and central obesity person accounts for 37.5%, and Mild Obesity person accounts for 25%; The severe obesity person reducing 1-5kg accounts for 5.66%, and central obesity person accounts for 26.42%, and Mild Obesity person accounts for 67.92%, illustrates that the fat-reducing effect of fruits and vegetables probiotic tablet alignment degree of the present invention and severe obesity person is better.
Be in particular in:
1. fruits and vegetables probiotic tablet probio kind of the present invention is many, and Lactobacillus plantarum CGMCC NO.9405 production of lactic acid speed wherein can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate, and its prebiotic performance is more superior.
2. ultrasonic extraction, high-pressure pulse electric extract and biological enzymolysis combination of sciences by modified dietary fiber of the present invention, gained modified dietary fiber Soluble Fiber cellulose content is high, more easily utilized by probio, improve probio in the growth of enteron aisle and fertility, increase kind and the quantity of probio flora, reduce large intestine pH value, improve intestine microenvironment; High adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively prevent body weight for humans metal poisoning; The stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property.
3. Chinese herbal medicine extract of the present invention is significantly to suppress the Chinese herbal medicine of Gram-negative bacteria for raw material, adopt water extract-alcohol precipitation and biological enzymolysis and obtain, active constituent content is high, effectively instead of antibiotic utilization, improve body immunity, strengthen the phagocytosis of macrophage to harmful bacteria, inhibit the Growth and reproduction of harmful intestinal tract bacteria, simultaneously also for the Growth and reproduction of probio provides guarantee.
4. microwave drying and low-temperature grinding combine by fruit vegetable powder of the present invention, remain the nutriment such as color and luster own and cellulose, vitamin, fructose of high microsteping fruits and vegetables to greatest extent, good, with short production cycle compared with the quality of production of other fruit vegetable powder, efficiency is high, be conducive to probio utilize and strengthen intestines peristalsis, be conducive to harmful bacteria and noxious material to excrete, prevent the generation of constipation and symptom of diarrhea.
5. plant extracts of the present invention is significantly can promote lactobacter growth, increase the natural plants of viable lactic acid bacteria content and the higher raw-food material of leptin content obtains through water extract-alcohol precipitation and biological enzymolysis, use apple vinegar adjust ph, adopt most scientific extracting method, remain active ingredient wherein to greatest extent, leptin is replaced with raw-food material, with appetite-suppressing, strengthen capacity of self-regulation, reduce external absorption and the interior synthesis of body of fat, enhancing fat-reducing effect, is also simultaneously nutriment that beneficial bacteria of intestinal tract provides comprehensively, enriches.
6. tea extract of the present invention not only can provide the factor of effectively losing weight, and containing abundant Natural Antioxidants, improve the oxidation resistance of human body, eliminate free radical, improve immunity of organisms, delay body aging, slow down the anti-ageing burden of gut flora, be conducive to gut flora and grow up healthy and sound.
7. the simple combination of the probiotic effects of fruits and vegetables probiotic tablet of the present invention and significantly not each component function of fat-reducing effect, but composite, the synergistic comprehensive embodiment of science, mainly modify gut flora from increase probio kind and quantity, increase probio nutriment, the growth of suppression harmful bacteria and increase body immunity aspect, improve the probiotic and fat-reducing effect of product.
8. the powerful network of modified dietary fiber of the present invention and absorption affinity, by probiotic powder parcel wherein, add the bacteriostasis of Chinese herbal medicine extract, stability that the antioxidation etc. of tea extract enhances fruits and vegetables probiotic tablet, improve the probiotics viable bacteria survival rate of tablet.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of Lactobacillus plantarum pulvis:
(1) first order seed is cultivated: access in 500 ml shake flasks by Lactobacillus plantarum CGMCC NO.9405 bacterial classification, seed culture medium loading amount 100 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, seed culture medium loading amount 1000 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 5% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 18 hours;
(5) fermentation tank culture: it is 3 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 5% inoculum concentration, fermentation medium loading amount 2 tons, condition of culture cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 22 hours.The complete zymotic fluid that ferments staticly settles, be centrifugally precipitated thing, and then add the carrier of sediment quality 0.8 times, mix, namely 50 DEG C of fluidized bed dryings obtain Lactobacillus plantarum pulvis, and vehicle weight consists of: sour milk powder 25 parts, 12 parts, dextrin.
Described seed culture medium quality group becomes: casein 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, Tween 800.1%, K 2hPO 40.2%, MgSO 4.7H 2o 0.02%, MnSO 4.H 2o 0.005%, CaCO 32%, surplus is water, pH6.8.
Described fermentation medium quality group becomes: soybean protein 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, K 2hPO 40.2%, MgSO 4.7H 2o 0.02%, MnSO 4.H 2o 0.005%, CaCO 32%, surplus is water, pH6.8.
In described Lactobacillus plantarum pulvis, viable bacteria content is 9x10 12cfu/g.
2. the preparation of modified dietary fiber
The preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber, rice bran, wheat bran 10:7:4:3:2:1.5 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 250W, 40KHz condition ultrasonic extraction 12min, simultaneously in electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 250Hz condition; Be 5.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 30min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.2mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 7:6:4:3:2:2:0.4 Homogeneous phase mixing in mass ratio.
3. the preparation of fruit vegetable powder
The mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 13%, leek powder 10%, celery powder 7%, white gourd powder 7%, strawberry powder 5%, carrot meal 5%, papaya powder 5%, pineapple powder 5%, mung bean sprouts powder 5%, orange powder 3%, oranges and tangerines powder 3%, sweet orange powder 3%, coconut slurry powder 4%, Cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blueberry powder 2%;
Namely above-mentioned fruit vegetable powder Homogeneous phase mixing obtains fruit vegetable powder;
The preparation method of each fruit vegetable powder described is: put by fruits and vegetables in the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 12min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 6mm, the sheet of thickness 4mm or fourth, put in microwave dryer and carry out microwave drying 7min, until moisture is down to 6% under power 3000W, frequency 2000Hz, temperature 55 DEG C of condition, then in 25 DEG C, be crushed to granularity 0.2mm, pack and obtain fruit vegetable powder.
4. the preparation of plant extracts
The preparation method of described plant extracts is: count by weight, accurately take jerusalem artichoke 40 parts, Chinese yam 35 parts, 30 parts, balsam pear, 30 parts, almond, burdock 25 parts, 20 parts, lotus seeds, cassia seed 18 parts, onion 12 parts, asparagus 8 parts, being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, control temperature 80 DEG C keeps 3h, then 50 DEG C are cooled to, the mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by apple vinegar adjust ph, enzymolysis 3h, finally add the mixture of mixed material 1.8 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filter, filtrate reduced in volume, freeze drying, low-temperature grinding is to granularity 0.2mm, then the vitamin D of crushed material quality 0.02% is added, mix and obtain plant extracts,
The parts by weight of described mixed enzyme consist of: zytase 18 parts, pentosanase 18 parts, dextranase 15 parts, 15 parts, lipase, Pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts;
Described concentration of alcohol is 75%.
5. the preparation of tea extract
The preparation method of described tea extract is: put by commercially available tealeaves in the supersonic wave cleaning machine that 0.8% sodium bicarbonate solution is housed and clean 15min in 200W, 40KHz, then in the sterile malt dextrin in aqueous solution of 0.30MPa pressure lower surface even application mass percent concentration 1%, the mass ratio of tealeaves and the maltodextrin aqueous solution is: 100:5, sealing, rehydration 5h under room temperature; Then in-21 DEG C of freezing 20min; Pulverize immediately, granularity is 1mm; Tealeaves after pulverizing is placed in pill tank, adds the sterilized water of tea quality 8 times, room temperature 200W, 40KHz condition ultrasonic extraction 15min; Simultaneously in electric-field intensity 30KV/cm, burst length 500 μ S, carries out high-pressure pulse electric extraction under pulse frequency 300Hz condition; Be finally 5.5 by said extracted liquid lactic acid adjust ph, add the complex enzyme of mixture quality 1%, in 40 DEG C of low temperature enzymolysis 8h; Enzymolysis liquid filtration, filtrate reduced in volume, freeze drying, low-temperature grinding to granularity are that namely 0.2mm obtains tea extract;
Described complex enzyme is by pectase, cellulase, tannase and protease 1.5:3:3:2 Homogeneous phase mixing in mass ratio.
6. the preparation of Chinese herbal medicine extract
The preparation method of described Chinese herbal medicine extract is: count by weight, accurately takes tarragon 53 parts, 52 parts, garlic, Herba portulacae 50 parts, cordate houttuynia 49 parts, the root of large-flowered skullcap 40 parts, golden cypress 35 parts, hawthorn 35 parts, 30 parts, Radix Glycyrrhizae, kuh-seng 20 parts, rhizoma alismatis 9 parts, giant knotweed 7 parts, rheum officinale 4 parts, semen brassicae 3 parts, the tuber of multiflower knotweed 2 parts; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, control temperature 80 DEG C keeps 3h, is then cooled to 50 DEG C, the compounded enzyme preparate adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally adds the mixture of mixed material 1.8 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2.5:1.5, reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.2mm obtains Chinese herbal medicine extract;
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase 12 parts, zytase 18 parts, pentosanase 18 parts, Pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts, 6 parts, lipase;
Described concentration of alcohol is 75%.
Embodiment 2
A kind of fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight:
70 parts, probio pulvis, modified dietary fiber 40 parts, fruit vegetable powder 30 parts, compound sugar 25 parts, plant extracts 28 parts, sour milk powder 22 parts, tea extract 18 parts, Chinese herbal medicine extract 9 parts, sweetener 4 parts, dolomol 0.3 part;
Described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 50 parts, bifidobacterium longum 40 parts, bifidobacterium bifidum 30 parts, bifidobacterium infantis 30 parts, bifidobacterium adolescentis 18 parts, lactobacillus bulgaricus 18 parts, lactobacillus acidophilus 12 parts, Lactobacillus casei 9 parts, streptococcus thermophilus 9 parts, saccharomyces cerevisiae 8 parts, candida utili bacterium 5 parts; Above-mentioned except Lactobacillus plantarum pulvis, other is commercial commodity, and viable bacteria content is: 9x10 12cfu/g;
Described Lactobacillus plantarum pulvis, modified dietary fiber, fruit vegetable powder, plant extracts, tea extract, Chinese herbal medicine extract are embodiment 1 to be prepared;
Described compound sugar parts by weight consist of FOS 45 parts, stachyose 35 parts, gossypose 30 parts, xylo-oligosaccharide 17 parts, galactooligosaccharide 12 parts, soyabean oligosaccharides 12 parts, oligoisomaltose 12 parts, oligomeric maltose 10 parts;
Described sour milk powder is commercial commodity, and viable lactic acid bacteria content is 5x10 11cfu/g;
Described sweetener is antierythrite, xylitol, Aspartame mix with arbitrary proportion;
The preparation method of described fruits and vegetables probiotic tablet, comprise the steps: accurately to take each component raw material by above-mentioned formula, first by probio pulvis and modified dietary fiber Homogeneous phase mixing 25min, then Chinese herbal medicine extract is added successively, tea extract, albumen powder, plant extracts, compound sugar, fruit vegetable powder Homogeneous phase mixing 12min, compound is added dry granulating machine granulation, collect 15-50 object particle, add sweetener successively, the raw material of directly compressible is obtained after dolomol mixing, drop into tabletting machine, adjustment pressure is 30kn, obtain fruits and vegetables probiotic tablet, airtight preservation at the temperature of 25 DEG C, place that conventionally to detect viable count in tablet and calculate survival rate after 12 months be 89%.
Embodiment 3
A kind of fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight:
60 parts, probio pulvis, modified dietary fiber 35 parts, fruit vegetable powder 25 parts, compound sugar 20 parts, plant extracts 25 parts, albumen powder 18 parts, tea extract 15 parts, Chinese herbal medicine extract 8 parts, sweetener 2 parts, dolomol 0.2 part;
Described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 45 parts, bifidobacterium longum 35 parts, bifidobacterium bifidum 25 parts, bifidobacterium infantis 25 parts, bifidobacterium adolescentis 15 parts, lactobacillus bulgaricus 15 parts, lactobacillus acidophilus 10 parts, Lactobacillus casei 8 parts, streptococcus thermophilus 8 parts, saccharomyces cerevisiae 5 parts, candida utili bacterium 4 parts; Above-mentioned except Lactobacillus plantarum pulvis, other is commercial commodity, and viable bacteria content is: 8x10 12cfu/g;
Described Lactobacillus plantarum pulvis, modified dietary fiber, fruit vegetable powder, plant extracts, tea extract, Chinese herbal medicine extract are embodiment 1 to be prepared;
Preferably, described compound sugar parts by weight consist of FOS 40 parts, stachyose 30 parts, gossypose 20 parts, xylo-oligosaccharide 16 parts, galactooligosaccharide 10 parts, soyabean oligosaccharides 10 parts, oligoisomaltose 10 parts, oligomeric maltose 8 parts;
Described albumen powder percentage by weight consists of: sour milk powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%;
Described sour milk powder is commercial commodity, and viable lactic acid bacteria content is 3x10 11cfu/g;
Described sweetener is antierythrite, xylitol, maltitol, D-sorbite, stevioside, mogroside, Aspartame mix with arbitrary proportion;
The preparation method of described fruits and vegetables probiotic tablet is with embodiment 2, and airtight preservation at the temperature of 25 DEG C, places that conventionally to detect viable count in tablet and calculate survival rate after 12 months be 85%.
Embodiment 4
A kind of fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight:
80 parts, probio pulvis, modified dietary fiber 45 parts, fruit vegetable powder 35 parts, compound sugar 30 parts, plant extracts 30 parts, albumen powder 25 parts, tea extract 20 parts, Chinese herbal medicine extract 10 parts, sweetener 5 parts, dolomol 0.5 part;
Described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 55 parts, bifidobacterium longum 45 parts, bifidobacterium bifidum 35 parts, bifidobacterium infantis 35 parts, bifidobacterium adolescentis 20 parts, lactobacillus bulgaricus 20 parts, lactobacillus acidophilus 15 parts, Lactobacillus casei 10 parts, streptococcus thermophilus 10 parts, saccharomyces cerevisiae 10 parts, candida utili bacterium 6 parts; Above-mentioned except Lactobacillus plantarum pulvis, other is commercial commodity, and viable bacteria content is: 7x10 12cfu/g;
Described Lactobacillus plantarum pulvis, modified dietary fiber, fruit vegetable powder, plant extracts, tea extract, Chinese herbal medicine extract are embodiment 1 to be prepared;
Described compound sugar parts by weight consist of FOS 50 parts, stachyose 40 parts, gossypose 40 parts, xylo-oligosaccharide 18 parts, galactooligosaccharide 15 parts, soyabean oligosaccharides 15 parts, oligoisomaltose 15 parts, oligomeric maltose 12 parts;
Described albumen powder percentage by weight consists of: sour milk powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%;
Described sour milk powder is commercial commodity, and viable lactic acid bacteria content is 4x10 11cfu/g.
Described sweetener is stevioside, mogroside, Aspartame mix with arbitrary proportion;
The preparation method of described fruits and vegetables probiotic tablet is with embodiment 2, and airtight preservation at the temperature of 25 DEG C, places that conventionally to detect viable count in tablet and calculate survival rate after 12 months be 85%.
Embodiment 5
A kind of fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight:
80 parts, probio pulvis, modified dietary fiber 35 parts, fruit vegetable powder 35 parts, FOS 20 parts, plant extracts 30 parts, lactalbumin 18 parts, tea extract 20 parts, Chinese herbal medicine extract 10 parts, Aspartame 2 parts, dolomol 0.5 part;
Described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 55 parts, bifidobacterium longum 35 parts, bifidobacterium bifidum 35 parts, bifidobacterium infantis 25 parts, bifidobacterium adolescentis 20 parts, lactobacillus bulgaricus 15 parts, lactobacillus acidophilus 15 parts, Lactobacillus casei 10 parts, streptococcus thermophilus 10 parts, saccharomyces cerevisiae 10 parts, candida utili bacterium 6 parts; Above-mentioned except Lactobacillus plantarum pulvis, other is commercial commodity, and viable bacteria content is: 9x10 12cfu/g;
Described Lactobacillus plantarum pulvis, modified dietary fiber, fruit vegetable powder, plant extracts, tea extract, Chinese herbal medicine extract are embodiment 1 to be prepared;
The preparation method of described fruits and vegetables probiotic tablet, with embodiment 2 airtight preservation at the temperature of 25 DEG C, places that conventionally to detect viable count in tablet and calculate survival rate after 12 months be 83%.
The probiotic test of embodiment 6 fruits and vegetables probiotic tablet
The fruits and vegetables probiotic tablet embodiment of the present invention 2 prepared, being prepared into viable count with sterilized water is 2 × 10 10the probiotic solution of CFU/mL, saves backup in 4 DEG C;
1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10 -8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing), shake up rapidly after sterilizing.Again the test tube 2 that 10mL probiotic solution is housed is placed in 80-90 DEG C of water-bath and heats 15-25min, get the probiotic solution after heating and carry out ten times of stepwise dilutions to 10 -8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing) upper and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated 24h under 35 DEG C of conditions, calculate the quantity before and after heating.
Result shows, Viable detection reaches 88%.
2, the resistance test of SGF and intestinal juice: the hydrochloric acid 16.4mL adding distil water dilution of getting 100g/L, pH value is made to be respectively 1.5,2.5 and 3.5, get 100mL dilute hydrochloric acid solution, add 1g pepsin respectively, it is made fully to dissolve, obtain SGF, miillpore filter degerming (0.22 μm) is for subsequent use.Get potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, by 0.1moL/L sodium hydroxide solution adjust ph to 6.8; Separately get trypsase 10g, the 100mL that adds water makes dissolving, and after two liquid mixing, be diluted with water to 1000ml, obtain simulated intestinal fluid, miillpore filter degerming (0.22 μm) is for subsequent use.Get the probiotic solution that 1mL keeps and join (i.e. ten times of stepwise dilutions) in the SGF of 9mL, and fully mix on the oscillator rapidly, be then placed in 30-45 DEG C of quiescent culture 2-4h.Take out nutrient solution when 1h, 2h, 3h, 4h respectively and count remaining viable count immediately, compare with former viable count, result shows, Viable detection is 97%.Then each 1mL of the nutrient solution digesting different time is taken in simulated gastric fluid, being inoculated in 9mL pH value is respectively in the simulated intestinal fluid of 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, compare with former viable count, result shows that Viable detection is 99%.
3, simulating the resistance test of cholate: the solution making 1g/L with pancreatin, and the pig cholate adding 0.8% in the solution, is 8.0 with the NaOH adjustment pH of 10%, then uses 0.45 μm of micro-filtrate membrane filtration and degerming.The probiotic solution kept by 0.5mL is inoculated into 4.5mL and simulates in cholate, obtains nutrient solution, count remaining viable count after cultivating 24h.By nutrient solution ten times of stepwise dilutions to 10 in sterile saline -8, and pour into MRS, be then placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.
Embodiment 7 fruits and vegetables probiotics tablets mouse intestinal performance test
The fruits and vegetables probiotic tablet embodiment of the present invention 2 prepared, being prepared into viable count with sterilized water is 2 × 10 10the probiotic solution of CFU/mL, saves backup in 4 DEG C;
Choose common Kunming small white mouse 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage Lincomycin Hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepares the mouse model of intestinal bacilli illness.Model group mouse diet declines, and do not occur dead and phenomenon of significantly suffering from diarrhoea, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 intestinal bacilli illness mouse, be divided into 2 groups at random, one group 20 only as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept 10cfu/ml)/only, another 20 as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of small white mouse every day, weigh in the 8th, 21 day mouse to probiotic tablet treatment group and natural recovering group, calculate each group of weight average growth rate, result is as table 1; Within every 5 days, survey each group of stool in mice Escherichia coli quantity, calculate average, result is as table 2.Get stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL dilution with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet just.
Table 1 mouse Gain weight
Grouping Average starting weight (g/ only) Average end heavy (g/ only) Average growth rate (%)
Natural recovering group 20.69±1.33 27.34±1.59 32.14 a
Probiotic tablet treatment group 20.41±1.45 31.29±1.78 53.31 b
The situation of coliform count in table 2 stool in mice
Probiotic tablet treatment group Mouse Weight average growth rate (53.31%) is significantly higher than natural recovering group (32.14%); Solution rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 80.89%, significantly lower than natural recovering group (24.78%), show the probio rapid field planting in small white mouse enteron aisle in fruits and vegetables probiotic tablet of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogens such as Escherichia coli, and resident time is long, continues, effectively improve enteron aisle performance.
Embodiment 8 fruits and vegetables probiotic tablet of the present invention judge experiment
The fruits and vegetables probiotic tablet of inviting 24 people to prepare embodiment of the present invention 2-5 and commercially available two kinds of probiotic tablets are judged, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), mouthfeel (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 3:
Table 3 sensory evaluation statistics
Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).
Above result shows, any one all obviously will be better than commercially available common probiotic tablet to fruits and vegetables probiotic tablet prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, and also the consumer of applicable Different age group, different hierarchy of consumption eats simultaneously.
The fat-reducing effect of embodiment 9 fruits and vegetables probiotic tablet of the present invention
The nutrition probiotic tablet that the overweight people selecting 100 Different age group at random uses the embodiment of the present invention 2 to prepare, product specification: 32 x 0.4g; Same age bracket men and women half and half, wherein Mild Obesity person 56, central obesity person 32, severe obesity 12, morning, noon and afternoon every day three times, each 1.2g, warm boiled water 30 days is a cycle.Take one week after date continuously, experimenter's state of mind generally makes moderate progress, and all testing crew reflection anxieties obviously reduce; Well sleep quality is had than before experiment.Through health examination, the every health indicator of health is all better than the sign situation before testing, and experimenter's self-control strengthens, autokinetic movement time lengthening, and life, study and work have regularity more, enter eucyclic weight loss procedures.Experimenter's body weight is detected simultaneously, the results are shown in Table 4, table 5.
Table 4 Different age group body weight testing result
Changes of weight Increase more than 1kg Within increase and decrease 1kg Reduce 1-5kg Reduce 5kg-10kg Reduce more than 10kg Add up to
Under-18s 1 3 9 4 1 18
18-25 year 0 1 9 6 4 20
25-30 year 0 0 8 7 5 20
30-50 year 0 0 12 5 4 21
More than 50 years old 0 0 15 4 2 21
Add up to 1 4 53 26 16 100
Above result shows, the men and women crowd of fruits and vegetables probiotic tablet of the present invention to Different age group all has remarkable result, take a cycle (30 days), the number reducing more than 1kg is 95 people, fat-reducing effect reaches more than 95%, wherein reduces 1-5kg, 5-10kg, more than 10kg be respectively 53%, 26% and 16%; The more significant age bracket of fat-reducing effect be 25-50 year more than, wherein reduce the crowd of more than 10kg be mainly 25-30 year, be secondly 30-50 year crowd; The population of weight of more than 50 years old reduces main at below 10kg, and in a word, the overweight people that fruits and vegetables probiotic tablet of the present invention can be applicable to various all ages and classes eats, and has significant fat-reducing effect.
Table 5 different obesity phenotype body weight testing result
Changes of weight Increase more than 1kg Within increase and decrease 1kg Reduce 1-5kg Reduce 5kg-10kg Reduce more than 10kg Add up to
Slightly 1 1 36 10 4 52
Moderate 0 3 14 10 6 33
Seriously 0 0 3 6 6 15
Add up to 1 4 53 26 16 100
Above result shows, the severe obesity person reducing more than 10kg accounts for 37.5%, and central obesity person accounts for 37.5%, and Mild Obesity person accounts for 25%; The severe obesity person reducing 1-5kg accounts for 5.66%, and central obesity person accounts for 26.42%, and Mild Obesity person accounts for 67.92%, illustrates that the fat-reducing effect of fruits and vegetables probiotic tablet alignment degree of the present invention and severe obesity person is better.

Claims (10)

1. a fruits and vegetables probiotic tablet, prepared by the raw material of following parts by weight: probio pulvis 60-80 part, modified dietary fiber 35-45 part, fruit vegetable powder 25-35 part, compound sugar 20-30 part, plant extracts 25-30 part, albumen powder 18-25 part, tea extract 15-20 part, Chinese herbal medicine extract 8-10 part, sweetener 2-5 part, dolomol 0.2-0.5 part;
Described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber, rice bran, wheat bran;
Described plant extracts be with can promote lactobacter growth and leptin content higher natural plants obtain through extraction.
2. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, described probio pulvis is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 45-55 part, bifidobacterium longum 35-45 part, bifidobacterium bifidum 25-35 part, bifidobacterium infantis 25-35 part, bifidobacterium adolescentis 15-20 part, lactobacillus bulgaricus 15-20 part, lactobacillus acidophilus 10-15 part, Lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 5-10 part, candida utili bacterium 4-6 part.
3. fruits and vegetables probiotic tablet as claimed in claim 2, it is characterized in that, the preparation method of described Lactobacillus plantarum pulvis comprises the steps: that Lactobacillus plantarum CGMCC NO.9405 bacterial classification is expanded cultivation through one, two, three shaking flask and first class seed pot obtains first class seed pot bacterial classification; With 5% inoculum concentration access secondary seed tank, fermentation medium loading amount is 2/3rds of tank appearance, condition of culture is temperature 37 DEG C, tank pressure 0.05MPa, incubation time 22 hours, zymotic fluid staticly settles, be centrifugally precipitated thing, then add the carrier of sediment quality 0.8 times, mix, namely 50 DEG C of fluidized bed dryings obtain Lactobacillus plantarum pulvis, vehicle weight consists of: sour milk powder 25 parts, 12 parts, dextrin.
4. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber, rice bran, wheat bran 7-12:6-9:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, simultaneously in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 6-8:4-7:3-5:2-4:1-3:1-3:0.3-0.5 Homogeneous phase mixing in mass ratio.
5. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, the mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 13%, leek powder 10%, celery powder 7%, white gourd powder 7%, strawberry powder 5%, carrot meal 5%, papaya powder 5%, pineapple powder 5%, mung bean sprouts powder 5%, orange powder 3%, oranges and tangerines powder 3%, sweet orange powder 3%, coconut slurry powder 4%, Cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blueberry powder 2%; The preparation method of each fruit vegetable powder described is: fruits and vegetables are put into the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer and carry out microwave drying 6-12min under power 3000W, frequency 2000Hz, temperature 50-60 DEG C condition, until moisture is down to 5-8%, then in 20-30 DEG C, be crushed to granularity 0.1-0.3mm, pack and obtain fruit vegetable powder.
6. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, the preparation method of described plant extracts is: count by weight, accurately take jerusalem artichoke 30-50 part, Chinese yam 30-40 part, balsam pear 20-40 part, almond 20-40 part, burdock 20-30 part, lotus seeds 15-25 part, cassia seed 15-20 part, onion 10-15 part, asparagus 5-10 part, being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of mixture 5 times of weight in container, control temperature 80 DEG C keeps 3h, then 50 DEG C are cooled to, the mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by apple vinegar adjust ph, enzymolysis 3h, finally add the mixture that mixed material 1.8 times of weight concentrations are 75% ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filter, filtrate reduced in volume, freeze drying, low-temperature grinding is to granularity 0.1-0.3mm, then the vitamin D of crushed material quality 0.01-0.03% is added, mix and obtain plant extracts,
The parts by weight of described mixed enzyme consist of: zytase 18 parts, pentosanase 18 parts, dextranase 15 parts, 15 parts, lipase, Pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts.
7. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, described albumen powder percentage by weight consists of: sour milk powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%.
8. fruits and vegetables probiotic tablet as claimed in claim 1, it is characterized in that, the preparation method of described Chinese herbal medicine extract is: count by weight, accurately take tarragon 50-55 part, garlic 50-55 part, Herba portulacae 45-55 part, cordate houttuynia 45-55 part, root of large-flowered skullcap 30-45 part, golden cypress 30-40 part, hawthorn 30-40 part, Radix Glycyrrhizae 25-35 part, kuh-seng 15-25 part, rhizoma alismatis 8-10 part, giant knotweed 6-8 part, rheum officinale 2-5 part, semen brassicae 2-5 part, tuber of multiflower knotweed 1-3 part; Respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of mixture 5 times of weight in container, control temperature 80 DEG C keeps 3h, then 50 DEG C are cooled to, the compound enzyme adding mixed material gross weight 8% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally adding mixed material 1.8 times of weight concentrations is the ethanol of 75% and the mixture of propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 4h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2.5:1.5, reduced pressure concentration, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains Chinese herbal medicine extract;
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase 12 parts, zytase 18 parts, pentosanase 18 parts, Pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucose oxidase 6 parts, acid phosphatase 8 parts, 6 parts, lipase.
9. the preparation method of fruits and vegetables probiotic tablet as described in as arbitrary in claim 1-8, it is characterized in that, comprise the steps: accurately to take each component raw material according to formula, first by probio pulvis and modified dietary fiber Homogeneous phase mixing 20-30min, then Chinese herbal medicine extract is added successively, tea extract, albumen powder, plant extracts, compound sugar, fruit vegetable powder Homogeneous phase mixing 10-15min, compound is added dry granulating machine granulation, collect 10-100 object particle, add sweetener successively, the raw material of directly compressible is obtained after dolomol mixing, drop into tabletting machine, adjustment pressure is 10-40kn, obtain fruits and vegetables probiotic tablet.
10. the preparation method of fruits and vegetables probiotic tablet as claimed in claim 9, it is characterized in that, the preparation method of described tea extract in turn includes the following steps: commercially available tealeaves is put into the supersonic wave cleaning machine that 0.8% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, then in the sterile malt dextrin in aqueous solution of 0.25-0.35MPa pressure lower surface even application mass percent concentration 0.5-1.5%, the mass ratio of tealeaves and the maltodextrin aqueous solution is: 100:0.5-15, sealing, rehydration 2-8h under room temperature; Then in-21--25 DEG C of freezing 10-30min; Pulverize immediately, granularity is 0.1-2mm; Tealeaves after pulverizing is placed in pill tank, adds tea quality 5-10 sterilized water doubly, room temperature 200W, 40KHz condition ultrasonic extraction 10-15min; Simultaneously in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be finally 4.5-6.5 by said extracted liquid lactic acid adjust ph, add the complex enzyme of mixture quality 0.5-1.5%, in 35-45 DEG C of low temperature enzymolysis 4-12h; Enzymolysis liquid filtration, filtrate reduced in volume, freeze drying, low-temperature grinding to granularity are that namely 0.1-0.3mm obtains tea extract;
Described complex enzyme is pectase, cellulase, tannase, protease 1-2:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.
CN201410658354.5A 2014-11-19 2014-11-19 A kind of fruit and vegerable probiotic tablet and preparation method thereof Active CN104489646B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410658354.5A CN104489646B (en) 2014-11-19 2014-11-19 A kind of fruit and vegerable probiotic tablet and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410658354.5A CN104489646B (en) 2014-11-19 2014-11-19 A kind of fruit and vegerable probiotic tablet and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104489646A true CN104489646A (en) 2015-04-08
CN104489646B CN104489646B (en) 2016-08-31

Family

ID=52931179

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410658354.5A Active CN104489646B (en) 2014-11-19 2014-11-19 A kind of fruit and vegerable probiotic tablet and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104489646B (en)

Cited By (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104799392A (en) * 2015-05-19 2015-07-29 河北金木药业集团有限公司 Fruit-vegetable solid beverage with function of coordinating intestines and stomach and preparation method for fruit-vegetable solid beverage
CN104926479A (en) * 2015-06-11 2015-09-23 天津中天精科科技有限公司 Fermentation medium for high-activity Pleurotus eryngii Quel. liquid strain
CN104938212A (en) * 2015-06-11 2015-09-30 天津中天精科科技有限公司 Pleurotus eryngii liquid strain with robust mycelia and fermentation method thereof
CN104970101A (en) * 2015-07-28 2015-10-14 天津中天精科科技有限公司 Probiotics troche and preparation method thereof
CN104982526A (en) * 2015-07-28 2015-10-21 天津天绿健科技有限公司 Yoghourt slice and preparation method thereof
CN104982219A (en) * 2015-06-11 2015-10-21 天津中天精科科技有限公司 Pleurotus eryngii liquid spawn with evenly distributed mycelium pellet and fermentation method thereof
CN104982530A (en) * 2015-07-28 2015-10-21 天津中天精科科技有限公司 Fruit paste troche containing lactic acid bacteria and preparation method of fruit paste troche
CN104996565A (en) * 2015-07-28 2015-10-28 天津中天精科科技有限公司 Viable bacteria containing fruit pulp food and preparation method thereof
CN105028638A (en) * 2015-07-28 2015-11-11 邵素英 Fruit and vegetable lactic acid bacteria tablet and preparation method thereof
CN105028646A (en) * 2015-07-28 2015-11-11 邵素英 Freeze-dried tablets and preparation method thereof
CN105028637A (en) * 2015-07-28 2015-11-11 邵素英 Fruit puree tablet containing probiotics and preparation method for fruit puree tablet
CN105028640A (en) * 2015-07-28 2015-11-11 邵素英 Fruit puree food and preparation method thereof
CN105053218A (en) * 2015-07-28 2015-11-18 天津中天精科科技有限公司 Fruit paste-lactobacillus tablet and preparation method thereof
CN105076439A (en) * 2015-07-16 2015-11-25 北京市绿友食品有限公司 Fruit puree and yogurt freeze-dried tablet and preparation method thereof
CN105076413A (en) * 2015-07-28 2015-11-25 天津中天精科科技有限公司 Fruit and vegetable tablet containing lactic acid bacteria and preparation method of fruit and vegetable tablet
CN105076440A (en) * 2015-07-16 2015-11-25 北京市绿友食品有限公司 Freeze-dried fruit and vegetable yoghurt tablet and preparation method thereof
CN105104529A (en) * 2015-07-28 2015-12-02 天津中天精科科技有限公司 Lactobacillus food and preparation method thereof
CN105123932A (en) * 2015-07-28 2015-12-09 天津天绿健科技有限公司 Fruit and vegetable probiotic tablet and preparation method thereof
CN105123924A (en) * 2015-07-28 2015-12-09 邵素英 Fruit paste probiotic tablet and preparation method thereof
CN105123934A (en) * 2015-07-28 2015-12-09 邵素英 Fruit paste foodstuff containing probiotics and preparation method thereof
CN105123933A (en) * 2015-07-28 2015-12-09 邵素英 Lactic acid bacterium tablet and preparation method thereof
CN105123931A (en) * 2015-07-28 2015-12-09 邵素英 Probiotic foodstuff and preparation method thereof
CN105124707A (en) * 2015-10-25 2015-12-09 林爱玉 Plant-fermentation healthcare drink
CN105123925A (en) * 2015-07-28 2015-12-09 天津中天精科科技有限公司 Freeze-dried foodstuff and preparation method thereof
CN105685345A (en) * 2016-01-29 2016-06-22 邹涛 Stachyose series food for children
CN105707235A (en) * 2016-01-29 2016-06-29 广西壮族自治区农业科学院农产品加工研究所 Intestine moistening and bowel relaxing yogurt drink and preparation method thereof
CN105767876A (en) * 2016-03-10 2016-07-20 内蒙古三主粮天然燕麦产业股份有限公司 Preparation method of oat bran-fermented chewing tablets
EP3111780A1 (en) * 2015-07-03 2017-01-04 Analyticon Discovery GmbH Natural stilbenes as sweeteners or sweetener enhancer
CN106387569A (en) * 2016-08-31 2017-02-15 广东佳业食品股份有限公司 Concentrated fermented fruit and vegetable juice and preparation method thereof
CN106418060A (en) * 2016-08-27 2017-02-22 宁波希诺亚海洋生物科技有限公司 Health drink for relieving acute gastroenteritis and preparation method of health drink
CN106901265A (en) * 2017-01-23 2017-06-30 广西育运农业开发有限公司 A kind of perfume lemon tablet and preparation method thereof
CN106962939A (en) * 2017-04-11 2017-07-21 姜红成 Prepare the fermentation composition and preparation method of the plant enzyme with weight-reducing degreasing effect
CN107307417A (en) * 2017-06-17 2017-11-03 深圳市领治医学科技有限公司 A kind of type 2 diabetes patient's nutriment
CN107455744A (en) * 2017-08-22 2017-12-12 绵阳市润土农业科技开发有限公司 A kind of fementative composition ferment and preparation method thereof in vivo
CN107568383A (en) * 2017-08-25 2018-01-12 上海应用技术大学 A kind of green tea probiotics effervescent tablet and preparation method thereof
CN107981335A (en) * 2017-12-05 2018-05-04 爱可道生物科技有限公司 A kind of globe artichoke enzyme film and preparation method thereof
CN108208185A (en) * 2018-03-12 2018-06-29 海南大学 A kind of preparation method of high-performance coconut palm slurry
CN108523043A (en) * 2017-05-16 2018-09-14 江苏派乐滋食品有限公司 A kind of preparation method of fruits and vegetables probiotics tablets
CN108902628A (en) * 2018-07-20 2018-11-30 殷金玲 A kind of anti-inflammatory probiotics solid beverage of children
CN110973630A (en) * 2019-12-20 2020-04-10 美益添生物医药(武汉)有限公司 Composition for resisting intestinal aging and application thereof
CN111374302A (en) * 2018-12-28 2020-07-07 江苏维乐益生食品科技有限公司 Probiotic celery powder and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1192360A (en) * 1997-08-01 1998-09-09 北京东方百信生物技术有限公司 Medicinal prepn. contg. beneficial bacteria named 'Junyikang' and method for preparing same
CA2551581A1 (en) * 2003-12-24 2005-07-14 Ferrosan A/S Probiotic tablet formulations
CN103704423A (en) * 2014-01-02 2014-04-09 绿锦发展(福建)有限公司 Preparation method for tea extract
CN104095180A (en) * 2013-04-12 2014-10-15 中国科学院烟台海岸带研究所 Probiotics inulin sheet and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1192360A (en) * 1997-08-01 1998-09-09 北京东方百信生物技术有限公司 Medicinal prepn. contg. beneficial bacteria named 'Junyikang' and method for preparing same
CA2551581A1 (en) * 2003-12-24 2005-07-14 Ferrosan A/S Probiotic tablet formulations
CN104095180A (en) * 2013-04-12 2014-10-15 中国科学院烟台海岸带研究所 Probiotics inulin sheet and preparation method thereof
CN103704423A (en) * 2014-01-02 2014-04-09 绿锦发展(福建)有限公司 Preparation method for tea extract

Cited By (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104799392B (en) * 2015-05-19 2017-08-01 河北金木药业集团有限公司 A kind of fruits and vegetables solid beverage with coordinating intestines and stomach function and preparation method thereof
CN104799392A (en) * 2015-05-19 2015-07-29 河北金木药业集团有限公司 Fruit-vegetable solid beverage with function of coordinating intestines and stomach and preparation method for fruit-vegetable solid beverage
CN104926479A (en) * 2015-06-11 2015-09-23 天津中天精科科技有限公司 Fermentation medium for high-activity Pleurotus eryngii Quel. liquid strain
CN104938212A (en) * 2015-06-11 2015-09-30 天津中天精科科技有限公司 Pleurotus eryngii liquid strain with robust mycelia and fermentation method thereof
CN104938212B (en) * 2015-06-11 2018-05-11 宁夏天绿健知识产权运营有限公司 A kind of pleurotus eryngii liquid strain and its fermentation process of mycelia stalwartness
CN104982219A (en) * 2015-06-11 2015-10-21 天津中天精科科技有限公司 Pleurotus eryngii liquid spawn with evenly distributed mycelium pellet and fermentation method thereof
WO2017005515A1 (en) * 2015-07-03 2017-01-12 Analyticon Discovery Gmbh Natural stilbenes as sweeteners or sweetener enhancer
EP3111780A1 (en) * 2015-07-03 2017-01-04 Analyticon Discovery GmbH Natural stilbenes as sweeteners or sweetener enhancer
AU2016290335B2 (en) * 2015-07-03 2020-05-14 Analyticon Discovery Gmbh Natural stilbenes as sweeteners or sweetener enhancer
US10653172B2 (en) 2015-07-03 2020-05-19 Analyticon Discovery Gmbh Natural stilbenes as sweeteners or sweetener enhancer
CN107734976A (en) * 2015-07-03 2018-02-23 阿纳里蒂科探索有限责任公司 Natural stilbene compound is as sweetener or sweetness enhancers
CN105076439A (en) * 2015-07-16 2015-11-25 北京市绿友食品有限公司 Fruit puree and yogurt freeze-dried tablet and preparation method thereof
CN105076440A (en) * 2015-07-16 2015-11-25 北京市绿友食品有限公司 Freeze-dried fruit and vegetable yoghurt tablet and preparation method thereof
CN105028638A (en) * 2015-07-28 2015-11-11 邵素英 Fruit and vegetable lactic acid bacteria tablet and preparation method thereof
CN105028646A (en) * 2015-07-28 2015-11-11 邵素英 Freeze-dried tablets and preparation method thereof
CN105053218A (en) * 2015-07-28 2015-11-18 天津中天精科科技有限公司 Fruit paste-lactobacillus tablet and preparation method thereof
CN105104529A (en) * 2015-07-28 2015-12-02 天津中天精科科技有限公司 Lactobacillus food and preparation method thereof
CN105123932A (en) * 2015-07-28 2015-12-09 天津天绿健科技有限公司 Fruit and vegetable probiotic tablet and preparation method thereof
CN105123924A (en) * 2015-07-28 2015-12-09 邵素英 Fruit paste probiotic tablet and preparation method thereof
CN105123934A (en) * 2015-07-28 2015-12-09 邵素英 Fruit paste foodstuff containing probiotics and preparation method thereof
CN105123933A (en) * 2015-07-28 2015-12-09 邵素英 Lactic acid bacterium tablet and preparation method thereof
CN105123931A (en) * 2015-07-28 2015-12-09 邵素英 Probiotic foodstuff and preparation method thereof
CN104970101A (en) * 2015-07-28 2015-10-14 天津中天精科科技有限公司 Probiotics troche and preparation method thereof
CN105123925A (en) * 2015-07-28 2015-12-09 天津中天精科科技有限公司 Freeze-dried foodstuff and preparation method thereof
CN105028640A (en) * 2015-07-28 2015-11-11 邵素英 Fruit puree food and preparation method thereof
CN105028637A (en) * 2015-07-28 2015-11-11 邵素英 Fruit puree tablet containing probiotics and preparation method for fruit puree tablet
CN105076413A (en) * 2015-07-28 2015-11-25 天津中天精科科技有限公司 Fruit and vegetable tablet containing lactic acid bacteria and preparation method of fruit and vegetable tablet
CN104996565A (en) * 2015-07-28 2015-10-28 天津中天精科科技有限公司 Viable bacteria containing fruit pulp food and preparation method thereof
CN104982530A (en) * 2015-07-28 2015-10-21 天津中天精科科技有限公司 Fruit paste troche containing lactic acid bacteria and preparation method of fruit paste troche
CN104982526A (en) * 2015-07-28 2015-10-21 天津天绿健科技有限公司 Yoghourt slice and preparation method thereof
CN105124707B (en) * 2015-10-25 2018-06-12 林爱玉 A kind of plant fermentation health drink
CN105124707A (en) * 2015-10-25 2015-12-09 林爱玉 Plant-fermentation healthcare drink
CN105685345A (en) * 2016-01-29 2016-06-22 邹涛 Stachyose series food for children
CN105707235A (en) * 2016-01-29 2016-06-29 广西壮族自治区农业科学院农产品加工研究所 Intestine moistening and bowel relaxing yogurt drink and preparation method thereof
CN105767876B (en) * 2016-03-10 2020-10-13 内蒙古三主粮天然燕麦产业股份有限公司 Preparation method of oat bran fermented chewable tablets
CN105767876A (en) * 2016-03-10 2016-07-20 内蒙古三主粮天然燕麦产业股份有限公司 Preparation method of oat bran-fermented chewing tablets
CN106418060A (en) * 2016-08-27 2017-02-22 宁波希诺亚海洋生物科技有限公司 Health drink for relieving acute gastroenteritis and preparation method of health drink
CN106387569A (en) * 2016-08-31 2017-02-15 广东佳业食品股份有限公司 Concentrated fermented fruit and vegetable juice and preparation method thereof
CN106901265A (en) * 2017-01-23 2017-06-30 广西育运农业开发有限公司 A kind of perfume lemon tablet and preparation method thereof
CN106962939A (en) * 2017-04-11 2017-07-21 姜红成 Prepare the fermentation composition and preparation method of the plant enzyme with weight-reducing degreasing effect
CN108523043A (en) * 2017-05-16 2018-09-14 江苏派乐滋食品有限公司 A kind of preparation method of fruits and vegetables probiotics tablets
WO2018227749A1 (en) * 2017-06-17 2018-12-20 深圳市领治医学科技有限公司 Nutriment for type 2 diabetes patient
CN107307417A (en) * 2017-06-17 2017-11-03 深圳市领治医学科技有限公司 A kind of type 2 diabetes patient's nutriment
CN107455744A (en) * 2017-08-22 2017-12-12 绵阳市润土农业科技开发有限公司 A kind of fementative composition ferment and preparation method thereof in vivo
CN107568383A (en) * 2017-08-25 2018-01-12 上海应用技术大学 A kind of green tea probiotics effervescent tablet and preparation method thereof
CN107981335A (en) * 2017-12-05 2018-05-04 爱可道生物科技有限公司 A kind of globe artichoke enzyme film and preparation method thereof
CN108208185A (en) * 2018-03-12 2018-06-29 海南大学 A kind of preparation method of high-performance coconut palm slurry
CN108902628A (en) * 2018-07-20 2018-11-30 殷金玲 A kind of anti-inflammatory probiotics solid beverage of children
CN111374302A (en) * 2018-12-28 2020-07-07 江苏维乐益生食品科技有限公司 Probiotic celery powder and preparation method thereof
CN110973630A (en) * 2019-12-20 2020-04-10 美益添生物医药(武汉)有限公司 Composition for resisting intestinal aging and application thereof

Also Published As

Publication number Publication date
CN104489646B (en) 2016-08-31

Similar Documents

Publication Publication Date Title
CN104489646B (en) A kind of fruit and vegerable probiotic tablet and preparation method thereof
CN104432092A (en) Probiotic tablet and preparation method thereof
CN104473183A (en) Weight-losing probiotic tablets and preparation method thereof
CN104509864B (en) A kind of have nutritional health food improving gastrointestinal function and preparation method thereof
CN104544086B (en) A kind of health food improving intestinal microbial population and preparation method thereof
CN104543679A (en) Probiotic-containing health food and preparation method thereof
CN105054040B (en) A kind of composition of probiotics fermention ginseng and its preparation method and application
CN107509906B (en) A kind of bitter buckwheat fruits and vegetables composite ferment powder and preparation method thereof
CN104770816A (en) Watermelon juice probiotic fermented beverage and preparation method thereof
CN104323059B (en) A kind of fermented vegetable fruit jam products and its preparation
CN104542977B (en) A kind of health beverages and preparation method containing yam extract and bifidobacterium bifidum
CN104872674A (en) Medlar and red date enzyme and preparation method thereof
CN105146614A (en) Functional Chinese dwarf cherry seed ferment, ferment beverage and production method of ferment beverage
CN105167072A (en) Production method of functional Chinese wolfberry fruit enzyme and product thereof
CN104970101A (en) Probiotics troche and preparation method thereof
CN105533544A (en) Natural fruit and vegetable enzyme product
CN104543680A (en) Health food for resisting fatigue and improving gastrointestinal functions and preparation method of health food
CN105123931A (en) Probiotic foodstuff and preparation method thereof
CN105028646A (en) Freeze-dried tablets and preparation method thereof
CN104323210B (en) A kind of fermentation tomato sauce product
CN104323211B (en) A kind of fermentation tomato sauce product preparation method
CN104489092A (en) Health drink containing grosvener siraitia extract and active bifidobacterium longum and preparation method for health drink containing grosvener siraitia extract and active bifidobacterium longum
CN104784261A (en) Cistanche enzyme and preparation process thereof
CN105028638A (en) Fruit and vegetable lactic acid bacteria tablet and preparation method thereof
CN105076440A (en) Freeze-dried fruit and vegetable yoghurt tablet and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CB03 Change of inventor or designer information

Inventor after: Gu Yingqiong

Inventor before: Li Yajun

CB03 Change of inventor or designer information
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20171201

Address after: 200000 Shanghai City, Jingan District Changde Road 545 Lane 174, room 705

Patentee after: Gu Yingqiong

Address before: Jiangling West Road, Wujiang District of Suzhou City, Jiangsu province 215299 new Lake Pearl City Lake Garden 20 building 2 unit 805

Patentee before: Li Yajun

TR01 Transfer of patent right

Effective date of registration: 20181114

Address after: 451450 Luxi, South Section of Construction Road, Zhongmou County, Zhengzhou City, Henan Province (Hongyi International Manor Area B)

Patentee after: Henan Hongyang Biotechnology Research Institute Co., Ltd.

Address before: Room 705, 174 Lane 545, Changde Road, Jing'an District, Shanghai 200000

Patentee before: Gu Yingqiong

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20200701

Address after: 210000 No. 111 Baizhu Road, Baima Town, Lishui District, Nanjing City, Jiangsu Province

Patentee after: Nanjing shennongyuan Bioengineering Co., Ltd

Address before: 451450 Luxi, South Section of Construction Road, Zhongmou County, Zhengzhou City, Henan Province (Hongyi International Manor Area B)

Patentee before: Henan Hongyang Biotechnology Research Institute Co.,Ltd.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20220329

Address after: 210000 room 209, science and innovation center, No. 111, Baima Avenue, Baima Town, Lishui District, Nanjing, Jiangsu Province

Patentee after: Nanjing Xuyou Food Technology Co.,Ltd.

Address before: No. 111, Baizhu Road, Baima Town, Lishui District, Nanjing City, Jiangsu Province, 210000

Patentee before: Nanjing shennongyuan Bioengineering Co.,Ltd.

TR01 Transfer of patent right