CN105123934A

CN105123934A - Fruit paste foodstuff containing probiotics and preparation method thereof

Info

Publication number: CN105123934A
Application number: CN201510449784.0A
Authority: CN
Inventors: 邵素英
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-07-28
Filing date: 2015-07-28
Publication date: 2015-12-09

Abstract

The invention discloses a fruit paste foodstuff containing probiotics and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit paste so as to prevent infection with infectious microbes in preparation process, improve fruit juice yield, increase the content of soluble cellulose in the fruit paste and prevent browning of the fruit paste; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit paste and the yoghurt. Eventually, the prepared fruit paste foodstuff containing probiotics has fine mouthfeel, uniform texture, stable state, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.55 * 10<11> to 2.52 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

Description

A kind of pulp food containing probio and preparation method thereof

Technical field

The present invention relates to pulp food, be specifically related to a kind of pulp food containing probio and preparation method thereof.

Background technology

Yoghourt is with fresh milk for raw material, adds beneficial bacterium fermentation after pasteurize again in milk, then through cooling filling a kind of milk product.Yoghourt not only remains the advantages such as the abundant nutrition of milk, and can also maximize favourable factors and minimize unfavourable ones through processing, becomes the nutrient and healthcare products being more suitable for people and needing.Yoghourt, except nutritious, also contains lactic acid bacteria, so have good health-care effect.Lactic acid bacteria safeguards the gut flora ecological balance, forms biological barrier, suppresses harmful bacteria to the invasion of enteron aisle.Lactic acid bacteria promotes the raised growth of intestines peristalsis and thalline by producing a large amount of SCFA, change osmotic pressure Constipation.Yoghourt contains multiple enzyme, can promote that enteron aisle is digested and assimilated.Lactic acid bacteria, by suppressing saprophytic bacteria in the growth of enteron aisle, suppresses the corrupt toxin produced, makes liver and brain from harm.Lactic acid bacteria is by suppressing saprophytic bacteria and the growth of some bacterium at enteron aisle.Also inhibits the carcinogen that these bacterium produce, reach the object of anti-cancer.Lactic acid bacteria can also produce the material that some strengthen immunologic function, improves immune function of human body.United States Department of Agriculture human nutriology research center researcher Ji En mayer represents, Yoghourt not only can improve intestinal environment, can also improve immunity of organisms.A research in Taiwan also finds, Yoghourt improves the result for the treatment of of some antiphlogistic.Good doctor Suo of HSPH of Harvard University epidemiological study person Ai Erwaluoa studies discovery, drinks the people that in the crowd of 2-3 part or more Yoghourt, hypertensive initiation potential is not drunk than those every day and reduces by 50%.

Agitating type fruit/vegetable yoghourt be with breast with dairy products for raw material, adding stabilizing agent, add lactic acid bacteria fermenting agent and form curdled milk, by stirring, adding the raw material such as Juice and the fermented dairy product made.Juice contains abundant nutrition, can be effectively human body replenishing vitamins, coordinate with Yoghourt, and Yoghourt not only can be made to have the distinctive local flavor of Juice, and Juice and Yoghourt nutritional labeling are supplemented mutually, and Yoghourt nutrition health-care functions is improved greatly.

But traditional the Shelf-Life of Yogurt is short, stores inconvenient, be not easy to long-distance transport; In addition, along with the raising of people's living standard and the enhancing of health care consciousness, exploitation health products become the large problem of food enterprise one further.Freeze-drying sheet (freeze-driedtablets) refers to that quantitative separating is in a fixed mold, and freeze-drying is anhydrated, obtained high porosity solid pharmaceutical preparation by major ingredient and auxiliary material mixing.At present, existing oral disintegrating tablet technology has desivac, pressed film method, direct compression process, wet method pressed disc method and wet granule compression tablet method etc., mainly adopts desivac and direct compression process at the oral disnitegration tablet of America and Europe's listing.Freeze-drying sheet has that disintegration rate is fast, mouthfeel good, realize stomach by mucosal transport before absorption, good stability, be beneficial to the advantages such as heat sensitive material processing.

At present, domestic in various types of sour milk tablet, adopt the few of desivac film-making, comprise in medical art, western medicine tablet, most employing be all other flaking method beyond desivac, and for lactic acid bacteria tablet number of viable being had to high requirement or probiotic tablet, desivac is a kind of effective ways improving number of viable beyond doubt, simultaneously, also can retain the natural color and luster of raw material, local flavor, mouthfeel and nutrition content to greatest extent, particularly important for fruit/vegetable yoghourt.Chinese patent CN102450322A discloses a kind of freeze-dried fruit yoghurt, is made up of freeze-dried fruit and Yoghourt base-material, and freeze-dried fruit and Yoghourt base-material splendid attire respectively, edible forward slip value freeze-dried fruit and Yoghourt base-material obtain freeze-dried fruit yoghurt.Chinese patent CN103099140B discloses a kind of mulberry fruit pulp freeze-drying sheet and technique, adopt the raw materials such as mulberry fruit fresh fruit, through washing fruit, making beating, allotment, homogeneous, filling, freeze, freeze-drying, the obtained mulberry fruit pulp such as nitrogen-filled seal freeze-drying sheet, with the high-pressure homogeneous refinement of secondary homogeneous mulberry fruit pulp component, adopt freeze drying technology reservation almost whole mulberry fruit sources anthocyanidin in addition to water, amino acid, mineral matter, the nutriments such as vitamin, add low-heat for sugared nutritive sweetener etc., the mulberry fruit effervescent tablet tool low sugar of preparation, low-heat, sour and sweet palatability, the smell of fruits is very sweet, the features such as preservation and good portability, direct-edible or reconstitute with water and drink.Chinese patent CN103109930B mono-kind adds the fruity probiotic yogurt sheet of silk gum antifreeze peptide, its raw material is made up of following component by weight: skimmed milk power 10 ~ 14 parts, sucrose 2 ~ 5 parts, sericin peptide taken 1 ~ 3 part, 1 ~ 4 part, fruit material, the active probiotic 0.1 ~ 0.6 part of freeze-drying and dolomol 0.1 ~ 0.4 part, this invention additionally provides the preparation method of product, technical process comprises: the preparation of skimmed milk food-grade culture medium, the fermentation of probio, the interpolation of silk gum antifreeze peptide, the vacuum freeze drying of acidified milk, the techniques such as pressed powder, silk gum antifreeze peptide and the fruit jelly dry powder being rich in B family vitamin is with the addition of in probiotic yogurt sheet, not only make number of live bacteria of probiotics in sour milk tablet high, the bacterium vital preservation time is longer, and improve its taste flavor.Qi Yongming carries out short summary to freeze-drying chip technology development in recent years and clinical advantage, and Zydis technology Problems existing is analyzed, freeze-drying sheet latest Progress is introduced simultaneously, paper freeze drying technology in international and domestic field of medicaments history, status quo and development trend, the defect that freeze drying technology exists and the technical issues that need to address (Qi Yongming. freeze-drying tablet preparation New research progress .JournalofChinaPrescriptionDrug2012.10 (4) .57-59).

Above-mentioned disclosed patent and technical literature lactic acid bacteria or probiotics viable bacteria content are still compared with low, fermented bacterium too poor, independent freeze-drying fruits and vegetables of conventional functionality and Yoghourt energy resource consumption consuming time is large, fruits and vegetables and Yoghourt preprocessing process heating power and shearing force cause nutriment to lose tradition processing that is large, pulp to make that Soluble Fiber cellulose content is lower, the poor stability shelf-life is short.

Therefore, prepare that a kind of number of viable is high, nutritious, quality is natural, the pulp food of long shelf-life is a kind of challenges newly to those skilled in the art.

Summary of the invention

Technical problem solved by the invention is the defect overcoming existing pulp Yoghourt and preparation method thereof, the leavening containing functional plants lactobacillus is with the addition of in Yoghourt fermentation process, there is the irritable Chinese herbal medicine extract of cold-and-heat resistent, occur that quality is thin after can effectively preventing Yoghourt from mixing with pulp, the stabilizing agent that layering and whey are separated out and containing multiple enzyme system and functional, the sprouted unpolished rice of bioactivator, improve Yoghourt fermentation technique and reach maximization to make lactic acid bacteria breed, improve the cold-and-heat resistent stress ability of lactic acid bacteria, to obtain high-quality, the multi-functional yoghurt of high viable bacteria content, and adopt non-thermal technology technology to carry out pretreatment to raw material milk and fruit juice, infect, improve juice yield and increase the content of the soluble cellulose of pulp to prevent process miscellaneous bacteria, prevent pulp brown stain, and the good cryoprotector of anti-freezing effect that the science that with the addition of when pulp mixes with Yoghourt is composite, to reduce the loss of the viable lactic acid bacteria content that freezing dry process causes, improves finished product viable bacteria content.Final obtained a kind of number of viable is high, nutritious, quality is natural, the pulp food of functional strong, long shelf-life.

Primary and foremost purpose of the present invention is to provide a kind of delicate mouthfeel, quality is homogeneous, in stable condition, number of viable is high, nutritious, quality is natural, the pulp food of functional strong, long shelf-life.

In order to achieve the above object, the present invention is by the following technical solutions:

Containing a pulp food for probio, the raw material primarily of following parts by weight obtains:

Cow's milk 50-70 part, fruit 30-50 part, sucrose 16-18 part, sprouted unpolished rice 6-9 part, extracts of Chinese herbal medicine 5-7 part, leavening 7-9 part, prebiotic factor 7-9 part, protective agent 6-8 part, stabilizing agent 0.1-0.3 part;

Described cow's milk is fresh cow milk, and acidity is not more than 18 ° of T, and dry matter content is not less than 11.6%.

Further, described fruit by one or more fresh fruits separately or combine;

Preferably, described fruit is that one or more fruit following form alone or in combination: strawberry, the red certain kind of berries, blueberry, raspberry, Huang Tao, cherry, Kiwi berry, peach, apple, pears, pineapple, banana, lichee, mango, grape, Malus spectabilis, Chinese pear-leaved crabapple, watermelon, "Hami" melon, muskmelon, coconut palm fruit, orange, mulberries.

Further, described sprouted unpolished rice take brown rice as raw material, through soaking, germinateing, the dry and a kind of functional stronger raw-food material of preparation, containing multiple enzyme system and functional, bioactivator;

Preferably, the preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature, power 200-400W, frequency 20-30KHz ultrasonic cleaning 5-10min in the supersonic wave cleaning machine that 0.1-0.3% sodium bicarbonate solution is housed, rinsing, to drain, put in 12-15 DEG C of water and soak 6-8h, ventilate once every 10-20min, ventilation pressure 0.10-0.13MPa, simultaneously in electric-field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 100-300Hz carries out high-pressure pulse electric process, until brown rice moisture content is 35-38%; Brown rice after immersion drains, in growing floor, carry out dark germination, dark germination temperature keep 20-24 DEG C, the dark germination time is 12-14h, period spray water be 42-44% to brown rice moisture content; Then putting temperature is soak 10-15min in the water of 35-45 DEG C to carry out killing embryo, until brown rice moisture content is to 43-45%; Brown rice after soaking again carries out dark germination in growing floor, and dark germination temperature keeps 23-25 DEG C, and the dark germination time is 18-22h; Brown rice after germinateing again is dry in frequency 2450MHz, power 3000W, temperature 50-60 DEG C, thickness of feed layer 2-4cm condition microwave irradiation, wherein microwave irradiation 10s, interval 10s, and being dried to moisture is 12-14%, packs and obtains sprouted unpolished rice;

Further, described extracts of Chinese herbal medicine significantly improves lactic acid bacteria to resist the Chinese herbal medicine of hot and cold irritability, disease resistance and immunity to prepare for raw material;

Preferably, the preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, take Radix Astragali 15-17 part, cordate houttuynia 15-17 part, barrenwort 13-16 part, hawthorn 12-15 part, honeysuckle 12-15 part, Ligusticum wallichii 8-12 part, root of kudzu vine 6-10 part, Radix Angelicae Sinensis 6-10 part, Radix Codonopsis 6-10 part, sterculia seed 5-8 part, fruit of glossy privet 3-7 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70 DEG C-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter, obtain the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85 DEG C-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, namely Homogeneous phase mixing obtains extracts of Chinese herbal medicine;

Described complex enzyme is dextranase, zytase, pentosanase, pectase 3-5:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described leavening comprise in following probio pulvis any one or multiple: such as: Lactobacillus plantarum (Lactobacillusplantarum), bifidobacterium bifidum (Bifidobacteriumbifidum), bifidobacterium infantis (B.infantis), bifidobacterium longum (B.longum), bifidobacterium breve (B.breve), bifidobacterium adolescentis (B.adolescentis), bifidobacterium lactis (Bifidobacteriumlactis), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), lactobacillus acidophilus (L.acidophilus), Lactobacillus casei (Lactobacilluscasei), Lactobacillus rhamnosus (Lactobacillusrhamnosus), streptococcus thermophilus (Streptococcusthermophilus),

Preferably, described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 30-40 part, bifidobacterium bifidum 25-35 part, bifidobacterium lactis 15-25 part, streptococcus thermophilus 15-25 part, bifidobacterium longum 15-20 part, bifidobacterium infantis 15-20 part, bifidobacterium adolescentis 13-18 part, lactobacillus bulgaricus 12-15 part, lactobacillus acidophilus 10-15 part, Lactobacillus casei 8-10 part, Lactobacillus rhamnosus 5-8 part;

Preferably, described Lactobacillus plantarum pulvis is that the bacterium that sets out is prepared according to a conventional method with Lactobacillus plantarum (Lactobacillusplantarum) QZ-5 disclosed in Chinese patent CN101974467A, and it is CGMCCNo.4200 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.This bacterial classification can produce multiple pathogen and the inhibited bacteriocin of spoilage organisms, and this bacteriocin has good heat endurance and absolute acid stability, and to protease-sensitive, antimicrobial spectrum is wide;

Preferably, described Lactobacillus plantarum pulvis is that the bacterium that sets out is prepared according to a conventional method with Lactobacillus plantarum (Lactobacillusplantarum) FM-LP1-1 disclosed in Chinese patent CN104531578A, and it is CGMCCNO.9488 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.This bacterial strain not only has effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, and this bacterium has excellent fermenting property and resistance to osmotic pressure performance, containing 200mg/LNaN0 ₂mRS nutrient solution in, the cell density inoculating this bacterium is 1.01 × 10 ⁷-1.54 × 10 ⁷cfu/ml, cultivates 24h, NaN0 under 25 DEG C of conditions ₂degradation rate reach more than 97.5%, cultivate 48h, NaN0 ₂degradation rate reach more than 99.1%; In MRS nutrient solution, cultivate 24h, the pH value of nutrient solution reaches 3.51;

More preferably, described Lactobacillus plantarum pulvis is that the pulvis prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488 mixes with arbitrary proportion;

More preferably, described Lactobacillus plantarum pulvis is the pulvis 1-3:4-6 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Further, described prebiotic factor weight consists of: oligoisomaltose 20-30 part, galactooligosaccharide 20-30 part, FOS 15-25 part, xylo-oligosaccharide 15-25 part, stachyose 10-15 part, soyabean oligosaccharides 10-15 part, gossypose 10-15 part, oligomeric maltose 8-12 part;

Further, described stabilizing agent is that any one or a few in modified dietary fiber, tremella polysaccharides, gelatin, pectin mixes with arbitrary proportion, occur after can effectively preventing Yoghourt from mixing with pulp that quality is thin, layering and whey separate out phenomenon, finally can make containing the pulp texture of food of probio, state and mouthfeel homogeneous, stable, fine and smooth;

Preferably, described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 10-15 part, tremella polysaccharides 6-10 part, gelatin 3-5 part, pectin 3-5 part;

Described modified dietary fiber is that the Soluble Fiber cellulose content obtained through physics, chemistry or biological method process by dietary fiber is high, biologically active strong, has cellulose that is important, positive role to human body beneficial flora, compared with full diet fiber, its biological action is more powerful;

Preferably, described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, Semen Tritici aestivi fiber, common oats fibre;

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, common oats fibre 4-6:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric process 10-15min under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;

Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 2-4:2-4:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio.

Further, described protective agent is for raw material with the animal or plant containing antifreeze protein, obtained through high-pressure pulse electric extraction, ultrasonic assistant Microwave Extraction and biological enzymolysis, effectively can improve lactic acid bacteria in pulp Yoghourt at freezing dry process Lactobacillus Survival, significantly improve the viable bacteria content of the pulp food containing probio;

Preferably, described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 7-9:6-8:2-4:1-3 Homogeneous phase mixing in mass ratio, adding mixed material quality 0.1-1 pH value is doubly that the lactic acid of 3.8-4.5 soaks 3-8h, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, crushed material particle diameter 0.5-3mm, then crushed material quality 10-20 water is doubly added, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high-pressure pulse electric process 20-30min is carried out under pulse frequency 200-300Hz condition, then under power 150-300W condition, carry out microwave irradiation in room temperature and extract 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 3-5%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 5-7:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio;

More preferably, described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s.

Another object of the present invention is to provide the preparation method of the described pulp food containing probio, comprises the steps:

1) Yoghourt preparation: cow's milk after clean breast under room temperature in electric-field intensity 30-40kV/cm, burst length 400-600 μ s, pulse frequency 300-500Hz carries out high-pressure pulse electric sterilization 10-20min, sprouted unpolished rice is crushed to particle diameter 0.1-0.3mm and sucrose, stabilizing agent and extracts of Chinese herbal medicine add in sterilization cow's milk successively, at 60-70 DEG C after abundant stirring and dissolving, homogeneous under 18-22MPa, then 32-35 DEG C is cooled in electric-field intensity 20-30kV/cm, burst length 200-400 μ s, pulse frequency 200-400Hz carries out high-pressure pulse electric sterilization 5-10min, adjusted to ph is 6.0-7.0, add the 20-30% ferment at constant temperature 0.5-1h of leavening quality, then with the ramp of 0.8-1.0 DEG C/min to 40-45 DEG C, add the 70-80% ferment at constant temperature 2-4h of leavening quality, last with the ramp of 0.6-0.8 DEG C/min to 50-55 DEG C, continuing fermentation to Yoghourt pH value is that 3.5-4.5 stops fermentation and to get product Yoghourt,

2) pulp preparation: fruit is put into the supersonic wave cleaning machine filling 0.3-0.5% sodium bicarbonate solution, 200-400W under room temperature, 35-45KHz cleans 5-10min, drain, pretreatment, broken, in electric-field intensity 35-45kV/cm under room temperature, burst length 800-1000 μ s, pulse frequency 200-300Hz high-pressure pulse electric process 5-10min, glue grinds, outlet pulp particle diameter is made to reach 100-200 μm, then under room temperature in electric-field intensity 20-30kV/cm, burst length 400-600 μ s, pulse frequency 200-400Hz high-pressure pulse electric process 3-5min, vacuum outgas obtains former pulp, add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 100-120ppm, 70-90ppm, 60-80ppm and 30-50ppm, in 45-55 DEG C of enzymolysis 1-3h, be warming up to 90-100 DEG C of insulation 3-5min, be cooled to 70-80 DEG C immediately, add the prebiotic factor while stirring, after abundant dissolving, be cooled to room temperature immediately, 80-100 eye mesh screen filters, and more than filtrate reduced in volume to solid content to 30% namely obtains pulp,

3) mix, filling: by step 1) Yoghourt, the step 2 that obtain) pulp that obtains and protective agent Homogeneous phase mixing, quantitative filling enters in mould container;

4) freeze: by the mould of filling pulp Yoghourt under the low temperature of-30 DEG C to-50 DEG C fully charge and in-30 DEG C preserve 6-18h;

5) freeze-drying: with the pulp Yoghourt freezing to deposit the stipulated time in freeze-drier by-25 DEG C/4-8h ,-15 DEG C/4-6h ,-5 DEG C/2-4h, the technique of 5 DEG C/2-6h and 20 DEG C/4-8h carries out frozen dried, terminate through 16-32h;

6) depanning, packing: destroy vacuum with clean, aseptic, dry nitrogen and take out dry pulp Yoghourt freeze-drying sheet at the operation room of clean health, aseptically, adopt and fill nitrogen and aluminum-plastic composite membrane gets product containing the pulp food of probio to the independent package encapsulation of pulp Yoghourt freeze-drying sheet.

Yoghourt viable lactic acid bacteria content prepared by said method is 7 × 10 ¹¹-9 × 10 ¹¹cFU/ml, the viable lactic acid bacteria content containing the pulp food of probio is 1.55 × 10 ¹¹-2.52 × 10 ¹¹cFU/g, under normal temperature, shelf-life 30-36 month.

Beneficial effect:

The present invention to contain at the leavening that Yoghourt fermentation process engineering is composite containing the pulp food of probio and can produce the Lactobacillus plantarum CGMCCNo.4200 of multiple pathogen and the inhibited bacteriocin of spoilage organisms and the Lactobacillus plantarum CGMCCNO.9488 with effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, the propagation of lactic acid bacteria in enteron aisle, field planting and detoxication and toxicant eliminating function ability can be significantly improved, effectively improve body immunity and gastrointestinal function, probiotic relative to existing leavening, functional stronger; The present invention adopts high-pressure pulse electric non-thermal sterilization technology to instead of traditional pasteurize in Yoghourt preparation process, remain the heat-sensitive nutrition in fresh milk to greatest extent, effectively prevent the living contaminants of process, stabilize and increase local flavor and the mouthfeel of Yoghourt, save the energy; There is the irritable Chinese herbal medicine extract of cold-and-heat resistent, can effectively prevent Yoghourt from mixing with pulp after occur that quality is thin, stabilizing agent that layering and whey are separated out and containing multiple enzyme system and sprouted unpolished rice that is functional, bioactivator, alternating temperature Yoghourt fermentation technique can make lactic acid bacteria breed to reach the cold-and-heat resistent stress ability of maximizations, raising lactic acid bacteria, can obtain that delicate mouthfeel, quality are homogeneous, in stable condition, the much higher functional yogurt of viable bacteria content; And adopt non-thermal technology technology to carry out pretreatment to fruit juice, infect, improve juice yield and increase the content of the soluble cellulose of pulp to prevent process miscellaneous bacteria, prevent pulp brown stain; And the good cryoprotector of anti-freezing effect that the science that with the addition of when pulp mixes with Yoghourt is composite, to reduce the loss of the viable lactic acid bacteria content that freezing dry process causes, improves finished product viable bacteria content.Final obtained a kind of delicate mouthfeel, quality is homogeneous, in stable condition, number of viable is high, nutritious, quality is natural, the pulp food containing probio of functional strong, long shelf-life.Viable lactic acid bacteria content containing the pulp food of probio is 1.55 × 10 ¹¹-2.52 × 10 ¹¹cFU/g, under normal temperature, shelf-life 30-36 month.Concrete test effect is shown in embodiment 7-12;

Concrete know-why is as follows:

1. the composite various lactobacillus agent of leavening science of the present invention, kind is many, function is complete, by force probiotic, particularly Lactobacillus plantarum CGMCCNo.4200 can produce multiple pathogen and the inhibited bacteriocin of spoilage organisms, and this bacteriocin has good heat endurance and absolute acid stability, and to protease-sensitive, antimicrobial spectrum is wide; Lactobacillus plantarum CGMCCNO.9488 not only has effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, and this bacterium has excellent fermenting property and resistance to osmotic pressure performance; Relative to existing ferment agent for sour milk fermenting power, probiotic, functional stronger.

2. stabilizing agent of the present invention is through concentrating on studies for many years, form by a large amount of scientific experiments is composite, not containing the chemical addition agent with any toxic and side effect, any potential harm can not be produced to human body, without addition restriction, be mainly natural plant raw material, foodsafety is strong, effective, occur after can effectively preventing Yoghourt from mixing with pulp that quality is thin, layering and whey separate out phenomenon, finally can make containing the pulp texture of food of probio, state and mouthfeel homogeneous, stable, fine and smooth.Ultrasonic extraction, high-pressure pulse electric extract and biological enzymolysis combination of sciences by modified dietary fiber wherein, gained modified dietary fiber Soluble Fiber cellulose content is high, more easily utilized by probio, improve probio in the growth of human body intestinal canal and fertility, increase kind and the quantity of probio flora, reduce human body intestinal canal pH value, improve human body intestinal canal micro-ecological environment; High adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively prevent body weight for humans metal poisoning; The stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property.Regulate and maintain the resident time of gut flora, strengthen the absorption and digestion ability of enteron aisle, improve immunity of organisms; Effective promotion gastrointestinal peristalsis, slows down and eliminates the bad reaction such as gasteremphraxis, abdominal distension; Powerful bury function can prevent environment (oxygen, temperature, illumination, water activity etc.) factor from, on the impact of product quality, stabilizing the biologically active of freeze-drying sheet, extending the shelf-life of freeze-drying sheet.

3. protective agent of the present invention with the animal or plant containing antifreeze protein for raw material, obtained through high-pressure pulse electric extraction, ultrasonic assistant Microwave Extraction and biological enzymolysis, loss of effective components is few, recovery rate is high, successful, effectively can improve lactic acid bacteria in pulp Yoghourt at freezing dry process Lactobacillus Survival, significantly improve the viable bacteria content of the pulp food containing probio; And raw material is single, composition simple, most importantly not containing chemical substance, foodsafety is strong.

4. sprouted unpolished rice of the present invention contains abundant bioactivator and functional materials, can be the nutriment that lactic acid bacteria provides abundant, the multiple enzyme system (protease, amylase, cellulase, lipase, phosphate etc.) wherein contained on the one hand can by the macromolecular substances of self: starch, protein, fat, cellulose change small-molecule substance into, what is more important can by the protein portion appropriateness degraded in Yoghourt, improve sour milk stability, the phase produces acid excessively after fermentation effectively to prevent lactic acid bacteria, affects yoghourt-flavored and mouthfeel; Sprouted unpolished rice germination percentage reaches more than 97%, the long 0.4-0.6mm of bud, length is homogeneous, sprout damage is only 1-2%, reduce 5-7% than existing germination technology loss, functional materials content is high, and wherein alpha-aminobutyric acid content is 303.8-315.8mg/100g, glutathione 17.2-18.6mg/100g, IP6 (IP ₆) 460.8-484.4mg/100g, dietary fiber 3.4-3.7mg/100g, add functional, stability and the palatability of Yoghourt.

5. the extracts of Chinese herbal medicine science prepared of the present invention is composite significantly improves lactic acid bacteria and resists cold, heat stress, the Chinese herbal medicine of disease resistance and immunity is raw material, through ultrasonic cleaning, low temperature enzymolysis, prepared by water extraction alcohol extracting and ultrasonic assistant Microwave Extraction, extract active constituent content is high, cost is low, effectively can strengthen the ability of the anti-low temperature of lactic acid bacteria and high temperature, adaptive temperature changing environment, for adaptation temperature-variable fermentation and freeze drying environment have established solid foundation, effective raising freeze-drying sheet viable bacteria content, simultaneously can lactic acid bacteria inhibiting after fermentation, prevent Yoghourt peracid from coordinating to keep yoghourt-flavored and mouthfeel, also can prevent freeze-drying sheet miscellaneous bacteria from encroaching on, extend the shelf-life of product.

6. pulp preparation process of the present invention adopts ultrasonic cleaning, effectively can reduce the introducing of process residues of pesticides and heavy metal ion, improves the foodsafety of fruit juice; Under room temperature after broken and glue grind, after high-pressure pulse electric process, its quality is more natural, and high, the blunt enzyme of rate of squeezing the juice is effective, miscellaneous bacteria content is extremely low, is significantly better than the quality index of commodity fruit juice; Adopt degasification technique, effectively reduce the dissolved oxygen content in juice production process, effectively improve the liquid phase stability of fruit juice; Glue mill and biological enzymolysis are organically combined, the complex enzyme zymohydrolysis of the fruit juice within the scope of certain particle size after science is composite, pigment of fruit juice tack more by force, stripping quantity is larger, dispersion force liquid phase that is stronger, pigment is more stable; By multiple non-thermal technology combine with technique process fruit juice, effectively prevent process miscellaneous bacteria from infecting, improve the content of the soluble cellulose of juice yield and increase pulp, prevent pulp brown stain, keep fruit juice natural quality.

7. the prebiotic factor of the present invention is not only lactic acid bacteria and provides abundant nutriment, and by composite for its science in pulp, works in coordination with protective agent the effect playing anti-freezing.

8. the present invention adopts non-thermal technology technology containing the preparation method of the pulp food of probio; operating efficiency is high; energy resource consumption is few; environmentally friendly, pollution-free, decrease the introducing of food additives and chemical assistant; effectively improve foodsafety; improve product quality and reduce production cost, achieve low-carbon (LC) productive target, can scale, industrialized development.Omnidistance employing low temperature processing techniques, optimize fermented bacterium and technique, with the addition of functional raw material, the freeze-drying sheet delicate mouthfeel of preparation, quality is homogeneous, in stable condition, number of viable is high, nutritious, quality is natural, by force functional, long shelf-life, biologically active is high, mouthfeel and local flavor and appetite strong, foodsafety is high, body immunity can be improved, resistance against diseases, endurance and anti-fatigue ability, improve functions of intestines and stomach, it should be noted that the eating effect that this contains the pulp food of probio is that each component is worked in coordination with mutually, interactional result, the not superposition of simple raw material function, the science of each raw material components is composite and extract, the effect produced is considerably beyond the superposition of each single component function and effect, there is advanced and practicality preferably.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of sprouted unpolished rice:

The preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature, power 300W, frequency 25KHz ultrasonic cleaning 8min in the supersonic wave cleaning machine that 0.2% sodium bicarbonate solution is housed, rinsing, to drain, put in 13 DEG C of water and soak 7h, ventilate once every 15min, ventilation pressure 0.12MPa, simultaneously in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 200Hz carries out high-pressure pulse electric process, until brown rice moisture content is 36%; Brown rice after immersion drains, in growing floor, carry out dark germination, and dark germination temperature keeps 22 DEG C, and the dark germination time is 13h, period spray water be 43% to brown rice moisture content; Then putting temperature is soak 12min in the water of 40 DEG C to carry out killing embryo, until brown rice moisture content to 44%; Brown rice after soaking again carries out dark germination in growing floor, and dark germination temperature keeps 24 DEG C, and the dark germination time is 20h; Brown rice after germinateing again is dry in frequency 2450MHz, power 3000W, temperature 55 DEG C, thickness of feed layer 3cm condition microwave irradiation, wherein microwave irradiation 10s, interval 10s, and being dried to moisture is 13%, packs and obtains sprouted unpolished rice;

The sprouted unpolished rice germination percentage prepared through said method reaches more than 99%, the long 0.5mm of bud, length is homogeneous, sprout damage is only 1%, than existing germination technology loss reduction by 7%, functional materials content is high, and wherein alpha-aminobutyric acid content is 315.8mg/100g, glutathione 18.6mg/100g, IP6 (IP ₆) 484.4mg/100g, dietary fiber 3.7mg/100g.

2. the preparation of extracts of Chinese herbal medicine:

The preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, takes the Radix Astragali 16 parts, cordate houttuynia 16 parts, barrenwort 15 parts, hawthorn 14 parts, honeysuckle 13 parts, Ligusticum wallichii 10 parts, the root of kudzu vine 8 parts, Radix Angelicae Sinensis 8 parts, Radix Codonopsis 8 parts, the sterculia seed 6 parts, the fruit of glossy privet 5 parts; Put in the supersonic wave cleaning machine filling 0.2% sodium bicarbonate solution and clean 12min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixed material, control temperature 80 DEG C keeps 3h, then being cooled to 45 DEG C, is 4.0 by lactic acid adjust ph, under power 200W condition, carry out Microwave Extraction 15min, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 1.0% carries out enzymolysis, be 6.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixed material 1.7 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 3.5h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3:2, namely Homogeneous phase mixing obtains extracts of Chinese herbal medicine;

Described complex enzyme is dextranase, zytase, pentosanase, pectase 4:3:2:2 Homogeneous phase mixing in mass ratio.

3. the preparation of modified dietary fiber:

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, common oats fibre 5:5:5:3 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 300W, 40KHz condition ultrasonic extraction 12min, then at electric-field intensity 30kV/cm, burst length 450 μ s, carries out high-pressure pulse electric process 12min under pulse frequency 300Hz condition; Be 5.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains modified dietary fiber;

Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 3:3:2:2:1.5 Homogeneous phase mixing in mass ratio.

The sprouted unpolished rice used in following examples 2-6, extracts of Chinese herbal medicine and modified dietary fiber are embodiment 1 to be prepared, and other is commercial commodity.

Embodiment 2

Containing a blueberry Yoghourt freeze-drying sheet for probio, the raw material primarily of following parts by weight obtains:

60 parts, cow's milk, blueberry 40 parts, sucrose 17 parts, sprouted unpolished rice 8 parts, extracts of Chinese herbal medicine 6 parts, leavening 8 parts, the prebiotic factor 8 parts, protective agent 7 parts, stabilizing agent 0.2 part;

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 35 parts, bifidobacterium bifidum 30 parts, bifidobacterium lactis 20 parts, streptococcus thermophilus 20 parts, bifidobacterium longum 18 parts, bifidobacterium infantis 18 parts, bifidobacterium adolescentis 15 parts, lactobacillus bulgaricus 13 parts, lactobacillus acidophilus 12 parts, Lactobacillus casei 9 parts, Lactobacillus rhamnosus 6 parts;

Described Lactobacillus plantarum pulvis is the pulvis 2:5 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described prebiotic factor weight consists of: oligoisomaltose 25 parts, galactooligosaccharide 25 parts, FOS 20 parts, xylo-oligosaccharide 20 parts, stachyose 12 parts, soyabean oligosaccharides 12 parts, gossypose 12 parts, oligomeric maltose 10 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 12 parts, tremella polysaccharides 8 parts, 4 parts, gelatin, pectin 4 parts.

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 8:7:3:2 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 0.5 times be 4.1 lactic acid soak 5h, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, crushed material particle diameter 2mm, then the water of crushed material quality 15 times is added, be 4.5 by lactic acid adjust ph, at electric-field intensity 30kV/cm under room temperature, burst length 400 μ s, high-pressure pulse electric process 25min is carried out under pulse frequency 250Hz condition, then under power 200W condition, carry out microwave irradiation in room temperature and extract 18min, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 4%, in 50 DEG C of enzymolysis 40min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 6:4:3:2:1.5 Homogeneous phase mixing in mass ratio;

Described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s.

Preparation method, comprises the steps:

1) Yoghourt preparation: cow's milk after clean breast under room temperature in electric-field intensity 35kV/cm, burst length 500 μ s, pulse frequency 400Hz carries out high-pressure pulse electric sterilization 15min, sprouted unpolished rice is crushed to particle diameter 0.2mm and sucrose, stabilizing agent and extracts of Chinese herbal medicine add in sterilization cow's milk successively, at 65 DEG C after abundant stirring and dissolving, homogeneous under 20MPa, then 33 DEG C are cooled in electric-field intensity 25kV/cm, burst length 300 μ s, pulse frequency 300Hz carries out high-pressure pulse electric sterilization 8min, adjusted to ph is 6.5, add 25% ferment at constant temperature 45min of leavening quality, then with the ramp to 42 DEG C of 0.9 DEG C/min, add 75% ferment at constant temperature 3h of leavening quality, last with the ramp to 53 DEG C of 0.7 DEG C/min, continuing fermentation to Yoghourt pH value is that 4.0 terminations fermentations get product Yoghourt,

2) blueberry pulp preparation: blueberry is put into the supersonic wave cleaning machine filling 0.4% sodium bicarbonate solution, under room temperature, 300W, 40KHz clean 8min, drain, broken, in electric-field intensity 40kV/cm, burst length 900 μ s, pulse frequency 250Hz high-pressure pulse electric process 8min under room temperature, glue grinds, outlet pulp particle diameter is made to reach 150 μm, then under room temperature in electric-field intensity 25kV/cm, burst length 500 μ s, pulse frequency 300Hz high-pressure pulse electric process 4min, vacuum outgas obtains former pulp; Add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 110ppm, 80ppm, 70ppm and 40ppm, in 50 DEG C of enzymolysis 2h, be warming up to 95 DEG C of insulation 4min, be cooled to 75 DEG C immediately, add the prebiotic factor while stirring, after abundant dissolving, be cooled to room temperature immediately, 90 eye mesh screens filter, and more than filtrate reduced in volume to solid content to 30% namely obtain blueberry pulp;

3) mix, filling: by step 1) Yoghourt, the step 2 that obtain) blueberry pulp that obtains and protective agent Homogeneous phase mixing, quantitative filling enters in mould container;

4) freeze: by the mould of filling blueberry pulp Yoghourt under the low temperature of-40 DEG C fully charge and in-30 DEG C preserve 12h;

5) freeze-drying: with the pulp Yoghourt freezing to deposit the stipulated time in freeze-drier by-25 DEG C/6h ,-15 DEG C/5h ,-5 DEG C/3h, the technique of 5 DEG C/4h and 20 DEG C/6h carries out frozen dried, terminate through 21h;

6) depanning, packing: destroy vacuum with clean, aseptic, dry nitrogen and take out drying blueberries freeze-drying sheet at the operation room of clean health, aseptically, adopt and fill nitrogen and aluminum-plastic composite membrane gets product containing the blueberry Yoghourt freeze-drying sheet of probio to the independent package encapsulation of blueberry Yoghourt freeze-drying sheet.

Yoghourt viable lactic acid bacteria content prepared by said method is 9 × 10 ¹¹cFU/ml, the viable lactic acid bacteria content of blueberry Yoghourt freeze-drying sheet is 2.52 × 10 ¹¹cFU/g, under normal temperature, the shelf-life is 36 months.

Embodiment 3

Contain the yellow peach of probio, apple, a bananas yoghourt freeze-drying sheet, the raw material primarily of following parts by weight obtains:

50 parts, cow's milk, Huang Tao 10 parts, apple 10 parts, 10 parts, banana, sucrose 16 parts, sprouted unpolished rice 6 parts, extracts of Chinese herbal medicine 5 parts, leavening 7 parts, the prebiotic factor 7 parts, protective agent 6 parts, stabilizing agent 0.1 part;

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 30 parts, bifidobacterium bifidum 25 parts, bifidobacterium lactis 15 parts, streptococcus thermophilus 15 parts, bifidobacterium longum 15 parts, bifidobacterium infantis 15 parts, bifidobacterium adolescentis 13 parts, lactobacillus bulgaricus 12 parts, lactobacillus acidophilus 10 parts, Lactobacillus casei 8 parts, Lactobacillus rhamnosus 5 parts;

Described Lactobacillus plantarum pulvis is the pulvis 1:4 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described prebiotic factor weight consists of: oligoisomaltose 20 parts, galactooligosaccharide 20 parts, FOS 15 parts, xylo-oligosaccharide 15 parts, stachyose 10 parts, soyabean oligosaccharides 10 parts, gossypose 10 parts, oligomeric maltose 8 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 10 parts, tremella polysaccharides 6 parts, 3 parts, gelatin, pectin 3 parts.

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 7:6:2:1 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 0.1 times be 3.8 lactic acid soak 3h, pulverize immediately after-18 DEG C of freezing 1h, freezing thickness of feed layer 3cm, crushed material particle diameter 0.5mm, then the water of crushed material quality 10 times is added, be 3.5 by lactic acid adjust ph, at electric-field intensity 25kV/cm under room temperature, burst length 300 μ s, high-pressure pulse electric process 20min is carried out under pulse frequency 200Hz condition, then under power 150W condition, carry out microwave irradiation in room temperature and extract 15min, under power 200W, frequency 30KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 3%, in 45 DEG C of enzymolysis 30min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 5:3:2:1:1 Homogeneous phase mixing in mass ratio.

Preparation method, comprises the steps:

1) Yoghourt preparation: cow's milk after clean breast under room temperature in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 300Hz carries out high-pressure pulse electric sterilization 10min, sprouted unpolished rice is crushed to particle diameter 0.1mm and sucrose, stabilizing agent and extracts of Chinese herbal medicine add in sterilization cow's milk successively, at 60 DEG C after abundant stirring and dissolving, homogeneous under 18MPa, then 32 DEG C are cooled in electric-field intensity 20kV/cm, burst length 200 μ s, pulse frequency 200Hz carries out high-pressure pulse electric sterilization 5min, adjusted to ph is 6.0, add 20% ferment at constant temperature 0.5h of leavening quality, then with the ramp to 40 DEG C of 0.8 DEG C/min, add 80% ferment at constant temperature 2h of leavening quality, last with the ramp to 50 DEG C of 0.6 DEG C/min, continuing fermentation to Yoghourt pH value is that 4.5 terminations fermentations get product Yoghourt,

2) pulp preparation: by yellow peach, the supersonic wave cleaning machine filling 0.3% sodium bicarbonate solution put into by apple and banana, 200W under room temperature, 35KHz cleans 5min, drain, Huang Tao, apple stoning, banana is removed the peel, cutting respectively, Homogeneous phase mixing, broken, in electric-field intensity 35kV/cm under room temperature, burst length 800 μ s, pulse frequency 200Hz high-pressure pulse electric process 5min, glue grinds, outlet pulp particle diameter is made to reach 100 μm, then under room temperature in electric-field intensity 20kV/cm, burst length 400 μ s, pulse frequency 200Hz high-pressure pulse electric process 3min, vacuum outgas obtains former pulp, add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 100ppm, 70ppm, 60ppm and 30ppm, in 45 DEG C of enzymolysis 1h, be warming up to 90 DEG C of insulation 3min, be cooled to 70 DEG C immediately, add the prebiotic factor while stirring, after abundant dissolving, be cooled to room temperature immediately, 80 eye mesh screens filter, and more than filtrate reduced in volume to solid content to 30% namely obtain pulp,

4) freeze: by the mould of filling yellow peach, apple, bananas yoghourt under the low temperature of-30 DEG C fully charge and in-30 DEG C preserve 6h;

5) freeze-drying: with freeze to deposit the yellow peach of stipulated time, apple, bananas yoghourt in freeze-drier by-25 DEG C/4h ,-15 DEG C/4h ,-5 DEG C/2h, the technique of 5 DEG C/2h and 20 DEG C/4h carries out frozen dried, terminate through 16h;

6) depanning, packing: destroy vacuum with clean, aseptic, dry nitrogen and take out dry yellow peach, apple, bananas yoghourt freeze-drying sheet at the operation room of clean health, aseptically, adopt and fill nitrogen and aluminum-plastic composite membrane yellow peach, apple, the independent package encapsulation of bananas yoghourt freeze-drying sheet are got product containing the yellow peach of probio, apple, bananas yoghourt freeze-drying sheet.

Yoghourt viable lactic acid bacteria content prepared by said method is 7 × 10 ¹¹the viable lactic acid bacteria content of CFU/ml, Huang Tao, apple, bananas yoghourt freeze-drying sheet is 1.55 × 10 ¹¹cFU/g, under normal temperature, 33 months shelf-lifves.

Embodiment 4

Containing mulberries, the watermelon Yoghourt freeze-drying sheet of probio, the raw material primarily of following parts by weight obtains:

70 parts, cow's milk, mulberries 25 parts, watermelon 25 parts, sucrose 18 parts, sprouted unpolished rice 9 parts, extracts of Chinese herbal medicine 7 parts, leavening 9 parts, the prebiotic factor 9 parts, protective agent 8 parts, stabilizing agent 0.3 part;

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 40 parts, bifidobacterium bifidum 35 parts, bifidobacterium lactis 25 parts, streptococcus thermophilus 25 parts, bifidobacterium longum 20 parts, bifidobacterium infantis 20 parts, bifidobacterium adolescentis 18 parts, lactobacillus bulgaricus 15 parts, lactobacillus acidophilus 15 parts, Lactobacillus casei 10 parts, Lactobacillus rhamnosus 8 parts;

Described Lactobacillus plantarum pulvis is the pulvis 3:6 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described prebiotic factor weight consists of: oligoisomaltose 30 parts, galactooligosaccharide 30 parts, FOS 25 parts, xylo-oligosaccharide 25 parts, stachyose 15 parts, soyabean oligosaccharides 15 parts, gossypose 15 parts, oligomeric maltose 12 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 15 parts, tremella polysaccharides 10 parts, 5 parts, gelatin, pectin 5 parts.

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 9:8:4:3 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 1 times be 4.5 lactic acid soak 8h, pulverize immediately after-22 DEG C of freezing 2h, freezing thickness of feed layer 5cm, crushed material particle diameter 3mm, then the water of crushed material quality 20 times is added, be 5.5 by lactic acid adjust ph, at electric-field intensity 35kV/cm under room temperature, burst length 500 μ s, high-pressure pulse electric process 30min is carried out under pulse frequency 300Hz condition, then under power 300W condition, carry out microwave irradiation in room temperature and extract 20min, under power 300W, frequency 40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 5%, in 55 DEG C of enzymolysis 50min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 7:5:4:3:2 Homogeneous phase mixing in mass ratio.

Preparation method, comprises the steps:

1) Yoghourt preparation: cow's milk after clean breast under room temperature in electric-field intensity 40kV/cm, burst length 600 μ s, pulse frequency 500Hz carries out high-pressure pulse electric sterilization 20min, sprouted unpolished rice is crushed to particle diameter 0.3mm and sucrose, stabilizing agent and extracts of Chinese herbal medicine add in sterilization cow's milk successively, at 70 DEG C after abundant stirring and dissolving, homogeneous under 22MPa, then 35 DEG C are cooled in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 400Hz carries out high-pressure pulse electric sterilization 10min, adjusted to ph is 7.0, add 30% ferment at constant temperature 1h of leavening quality, then with the ramp to 45 DEG C of 1.0 DEG C/min, add 80% ferment at constant temperature 4h of leavening quality, last with the ramp to 55 DEG C of 0.8 DEG C/min, continuing fermentation to Yoghourt pH value is that 3.5 terminations fermentations get product Yoghourt,

2) pulp preparation: by mulberries, the supersonic wave cleaning machine filling 0.5% sodium bicarbonate solution put into by watermelon, 400W under room temperature, 45KHz cleans 10min, drain, watermelon is removed the peel, remove seed, get flesh and mulberries Homogeneous phase mixing, broken, in electric-field intensity 45kV/cm under room temperature, burst length 1000 μ s, pulse frequency 300Hz high-pressure pulse electric process 10min, glue grinds, outlet pulp particle diameter is made to reach 200 μm, then under room temperature in electric-field intensity 30kV/cm, burst length 600 μ s, pulse frequency 400Hz high-pressure pulse electric process 5min, vacuum outgas obtains former pulp, add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 120ppm, 90ppm, 80ppm and 50ppm, in 55 DEG C of enzymolysis 3h, be warming up to 100 DEG C of insulation 5min, be cooled to 80 DEG C immediately, add the prebiotic factor while stirring, after abundant dissolving, be cooled to room temperature immediately, 100 eye mesh screens filter, and more than filtrate reduced in volume to solid content to 30% namely obtain pulp,

4) freeze: by the mould of filling mulberries, watermelon Yoghourt under the low temperature of-50 DEG C fully charge and in-30 DEG C preserve 18h;

5) freeze-drying: with freeze to deposit the mulberries of stipulated time, watermelon Yoghourt in freeze-drier by-25 DEG C/8h ,-15 DEG C/6h ,-5 DEG C/4h, the technique of 5 DEG C/6h and 20 DEG C/8h carries out frozen dried, terminate through 32h;

6) depanning, packing: destroy vacuum with clean, aseptic, dry nitrogen and take out dry mulberries, watermelon Yoghourt freeze-drying sheet at the operation room of clean health, aseptically, adopt and fill nitrogen and aluminum-plastic composite membrane gets product containing mulberries, the watermelon Yoghourt freeze-drying sheet of probio to mulberries, the independent package encapsulation of watermelon Yoghourt freeze-drying sheet.

Yoghourt viable lactic acid bacteria content prepared by said method is 8 × 10 ¹¹cFU/ml, the viable lactic acid bacteria content of mulberries, watermelon Yoghourt freeze-drying sheet is 2.12 × 10 ¹¹cFU/g, under normal temperature, 30 months shelf-lifves.

Embodiment 5

Containing a grape Yoghourt freeze-drying sheet for probio, the raw material primarily of following parts by weight obtains:

50 parts, cow's milk, grape 50 parts, sucrose 16 parts, sprouted unpolished rice 9 parts, extracts of Chinese herbal medicine 5 parts, leavening 9 parts, the prebiotic factor 7 parts, protective agent 8 parts, stabilizing agent 0.1 part;

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 30-40 part, bifidobacterium bifidum 25-35 part, bifidobacterium lactis 15-25 part, streptococcus thermophilus 15-25 part, bifidobacterium longum 15-20 part, bifidobacterium infantis 15-20 part, bifidobacterium adolescentis 13-18 part, lactobacillus bulgaricus 12-15 part, lactobacillus acidophilus 10-15 part, Lactobacillus casei 8-10 part, Lactobacillus rhamnosus 5-8 part;

Described Lactobacillus plantarum pulvis is the pulvis prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200.

Described prebiotic factor weight consists of: oligoisomaltose 20 parts, galactooligosaccharide 30 parts, FOS 15 parts, xylo-oligosaccharide 25 parts, stachyose 10 parts, soyabean oligosaccharides 15 parts, gossypose 10 parts, oligomeric maltose 12 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 10 parts, tremella polysaccharides 10 parts, 3 parts, gelatin, pectin 5 parts.

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 7:8:2:3 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 0.1 times be 4.5 lactic acid soak 3h, pulverize immediately after-22 DEG C of freezing 1h, freezing thickness of feed layer 3cm, crushed material particle diameter 3mm, then the water of crushed material quality 10 times is added, be 5.5 by lactic acid adjust ph, at electric-field intensity 25kV/cm under room temperature, burst length 500 μ s, high-pressure pulse electric process 20min is carried out under pulse frequency 200Hz condition, then under power 300W condition, carry out microwave irradiation in room temperature and extract 15min, under power 300W, frequency 40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 5%, in 45 DEG C of enzymolysis 50min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 5:5:2:3:1 Homogeneous phase mixing in mass ratio.

Preparation method, except grape carries out belt leather, the fragmentation of band seed, other processing step is with embodiment 2;

Yoghourt lactic acid bacteria probio content prepared by said method is 7 × 10 ¹¹-9 × 10 ¹¹cFU/ml, the viable lactic acid bacteria content of grape Yoghourt freeze-drying sheet is 2.48 × 10 ¹¹cFU/g, under normal temperature, 32 months shelf-lifves.

Embodiment 6

Containing pineapple, the mango yogurt freeze-drying sheet of probio, the raw material primarily of following parts by weight obtains:

70 parts, cow's milk, 15 parts, pineapple, mango 15 parts, sucrose 18 parts, sprouted unpolished rice 6 parts, extracts of Chinese herbal medicine 7 parts, leavening 7 parts, the prebiotic factor 9 parts, protective agent 6 parts, stabilizing agent 0.3 part;

Described Lactobacillus plantarum pulvis is the pulvis prepared according to a conventional method by Lactobacillus plantarum CGMCCNO.9488.

Described prebiotic factor weight consists of: oligoisomaltose 30 parts, galactooligosaccharide 20 parts, FOS 25 parts, xylo-oligosaccharide 15 parts, stachyose 15 parts, soyabean oligosaccharides 10 parts, gossypose 15 parts, oligomeric maltose 8 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 15 parts, tremella polysaccharides 6 parts, 5 parts, gelatin, pectin 3 parts.

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 9:6:4:1 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 1 times be 3.8 lactic acid soak 8h, pulverize immediately after-18 DEG C of freezing 2h, freezing thickness of feed layer 5cm, crushed material particle diameter 0.5mm, then the water of crushed material quality 20 times is added, be 3.5 by lactic acid adjust ph, at electric-field intensity 35kV/cm under room temperature, burst length 300 μ s, high-pressure pulse electric process 20min is carried out under pulse frequency 300Hz condition, then under power 300W condition, carry out microwave irradiation in room temperature and extract 15min, under power 300W, frequency 30KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 5%, in 45 DEG C of enzymolysis 50min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 7:3:4:1:2 Homogeneous phase mixing in mass ratio.

Preparation method, except pineapple peeling, cutting, mango stoning, outside mixing fragmentation, other processing step is with embodiment 3;

Yoghourt lactic acid bacteria probio content prepared by said method is 7 × 10 ¹¹-9 × 10 ¹¹cFU/ml, the viable lactic acid bacteria content of pineapple, mango yogurt freeze-drying sheet is 1.86 × 10 ¹¹cFU/g, under normal temperature, 35 months shelf-lifves.

Embodiment 7 protective agent effect test of the present invention

With the embodiment of the present invention 2 for test group; under other raw material and the identical condition of the condition such as component, technique; only protective agent kind is different; form blank group (not adding protective agent) and control group (sericin peptide taken is protective agent); before measuring freeze-drying and finally containing the viable bacteria content of the pulp food of probio, result is as table 1:

Table 1: different group viable bacteria content

Project	Before freeze-drying	Blank group	Control group	Test group
					Viable bacteria content (CFU/g)	2.81×10 ¹¹	6.84×10 ⁹	1.94×10 ¹⁰	2.52×10 ¹¹
Viable bacteria loss late (%)	0	98	93	10

Above result shows; use protective agent of the present invention as cryoprotector; the viable bacteria content of the pulp food containing probio can be significantly improved; under similarity condition; after cool-drying, test group viable bacteria content is nearly 13 times of control group; be 36 times of blank group, viable bacteria loss late and control group, reduce by 83% and 88% respectively compared with blank group, there is very good anti-freezing effect.

It should be noted that: embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 8 the present invention is containing the sensory evaluation test of the pulp food of probio

That invites 24 personnel to prepare the embodiment of the present invention 2 judges with the pulp food containing probio of commercially available two kinds of similar identical dates of manufacture containing the pulp food of probio, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), mouthfeel (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 2:

Table 2 sensory evaluation statistics

Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).

Above result shows, from outward appearance, quality, local flavor and mouthfeel, any one all obviously will be better than the commercially available pulp food containing probio to pulp food containing probio prepared by the present invention, particularly outward appearance, local flavor and mouthfeel are fabulous, and also the consumer of applicable Different age group, different hierarchy of consumption eats simultaneously.

It should be noted that: prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally containing the pulp food of probio, between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 9 fruit juice sense organ, health, physical and chemical quality indexes and enzymatic activity contrast test

Take watermelon as raw material, with the embodiment of the present invention 2 step 2) the obtained watermelon Normal juice of method is for test group, the watermelon juice that existing heating power protects look to adopt, prepared by blunt enzymatic process is for control group, respectively sense organ, physics and chemistry and sanitary index are carried out (by sanitary indexs such as low temperature recovery pulp detection total plate count, Escherichia coli, pathogenic bacteria, mould, yeast, remains of pesticide, heavy metals in fruit-vegetable juice beverage sanitary standard GB19297-2003 to the watermelon juice of test group and control group,), polyphenol oxidase enzymatic activity rate of descent detects, result is as table 3:

Table 3

Above result shows: watermelon Normal juice (test group) prepared by the present invention can retain watermelon natural colored and local flavor to greatest extent compared with prior art (control group), substantially close with the natural colored of watermelon, mouthfeel and local flavor, do not add any essence and pigment, soluble solid content is higher, has higher rate of squeezing the juice; Watermelon Normal juice pollution microbes kind prepared by the present invention is few, and content is low, and process contamination risk is little, although control group total plate count and coliform qualified, microbe species is many, content is higher, as not heat sterilization, there is the risk of later stage probiotics fermention failure; Juice preparation process of the present invention have employed ultrasonic cleaning to raw material, effectively reduce that agriculture is residual, the content of toxin and heavy metal ion, although watermelon Normal juice prepared by prior art is qualified, there is larger food security hidden danger, long-term edible meeting cause the serious harm of accumulation property to human body; Watermelon Normal juice polyphenol oxidase activity prepared by the present invention is low, effectively prevent the enzymatic browning of postorder process.

After testing, fruit juice prepared by embodiment of the present invention 3-6 and embodiment 2 sense organ, physics and chemistry and sanitary index, enzymatic activity rate of descent testing result there was no significant difference, have excellent foodsafety, non-biostability and microbial stability equally.

Embodiment 10 the present invention is containing the probiotic test of pulp food of probio

The pulp food containing the probio embodiment of the present invention 2 prepared, being prepared into viable count with sterilized water is 2 × 10 ¹⁰the probiotic solution of CFU/mL, saves backup in 4 DEG C;

1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing), shake up rapidly after sterilizing.Again the test tube 2 that 10mL probiotic solution is housed is placed in 80-90 DEG C of water-bath and heats 15-25min, get the probiotic solution after heating and carry out ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing) upper and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated 24h under 35 DEG C of conditions, calculate the quantity before and after heating.

Result shows, Viable detection reaches more than 88%.

2, the resistance test of SGF and intestinal juice: the hydrochloric acid 16.4mL adding distil water dilution of getting 100g/L, pH value is made to be respectively 1.5,2.5 and 3.5, get 100mL dilute hydrochloric acid solution, add 1g pepsin respectively, it is made fully to dissolve, obtain SGF, miillpore filter degerming (0.22 μm) is for subsequent use.Get potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, by 0.1moL/L sodium hydroxide solution adjust ph to 6.8; Separately get trypsase 10g, the 100mL that adds water makes dissolving, and after two liquid mixing, be diluted with water to 1000ml, obtain simulated intestinal fluid, miillpore filter degerming (0.22 μm) is for subsequent use.Get the probiotic solution that 1mL keeps and join (i.e. ten times of stepwise dilutions) in the SGF of 9mL, and fully mix on the oscillator rapidly, be then placed in 30-45 DEG C of quiescent culture 2-4h.Take out nutrient solution when 1h, 2h, 3h, 4h respectively and count remaining viable count immediately, compare with former viable count, result shows, Viable detection is 98%.Then each 1mL of the nutrient solution digesting different time is taken in simulated gastric fluid, being inoculated in 9mLpH value is respectively in the simulated intestinal fluid of 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, compare with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: the solution making 1g/L with pancreatin, and the pig cholate adding 0.8% in the solution, is 8.0 with the NaOH adjustment pH of 10%, then uses 0.45 μm of micro-filtrate membrane filtration and degerming.The probiotic solution kept by 0.5mL is inoculated into 4.5mL and simulates in cholate, obtains nutrient solution, count remaining viable count after cultivating 24h.By nutrient solution ten times of stepwise dilutions to 10 in sterile saline ^-8, and pour into MRS, be then placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.

Above result of the test shows, the present invention is stronger containing the probio probiotic (heat resistance, resistance to pH, bile tolerance) in the pulp food of probio, very be applicable to human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is large, effectively can improve gastrointestinal function.

Embodiment 11 the present invention is containing the pulp food mouse intestinal performance test of probio

Choose common Kunming small white mouse 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage Lincomycin Hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepares the mouse model of intestinal bacilli illness.Model group mouse diet declines, and do not occur dead and phenomenon of significantly suffering from diarrhoea, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 intestinal bacilli illness mouse, be divided into 2 groups at random, one group 20 only as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept ¹⁰cfu/ml)/only, another 20 as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of small white mouse every day, weighed in the 8th, 21 day to containing the pulp food treatment group of probio and the mouse of natural recovering group, calculate each group of weight average growth rate, result is as table 4; Within every 5 days, survey each group of stool in mice Escherichia coli quantity, calculate average, result is as table 5.Get stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL dilution with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet just.

Table 4 mouse Gain weight

Grouping	Average starting weight (g/ only)	Average end heavy (g/ only)	Average growth rate (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14 ^a
Treatment group	20.41±1.45	34.28±1.62	67.96 ^b

The situation of coliform count in table 5 stool in mice

Pulp food treatment group Mouse Weight average growth rate (67.96%) containing probio is significantly higher than natural recovering group (32.14%); Solution rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 97.07%, significantly lower than natural recovering group (24.78%), show that the present invention contains the probio rapid field planting in small white mouse enteron aisle in the pulp food of probio, form dominant microflora, and effectively suppress the growth and breeding of the pathogens such as Escherichia coli, and resident time is long, continues, effectively improve enteron aisle performance.

Embodiment 12 the present invention contains the pulp food of probio to the impact of immunity of organisms

1 experiment purpose

By exercise tolerance test (mouse forced swimming), checking the present invention is containing raising immunity, the antifatigue effect of the pulp food of probio.

2 experiment materials and reagent

2.1 for reagent thing:

The commercially available pulp food (G1) containing probio; The commercially available pulp food (G2) containing probio; Pulp food (G3-G7) containing probio prepared by embodiment of the present invention 2-6.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; The concentrated sulfuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.; Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. animal used as test

ICR mouse, ♂, cleaning grade, body weight 18-22g, is provided by Beijing Medical University's comparative medicine center, the free diet of experimental session mouse.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental facilities Co., Ltd of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer

5. experiment grouping

5.1 dosage groupings and given the test agent give the time: at random mouse is divided into 8 groups, often organize 10,1st group to the 7th group respectively to the medicine of G1 ~ G7,8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations: the 1st group to the 7th group: take 2.25g drug sample, be assigned to 150ml with distilled water; Blank group (the 8th group): distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of mouse root of the tail portion load 5% body weight, and the swimming of record mouse starts to the dead time, as mouse swimming time.

After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, eyeball blood sampling 0.5mL (not adding anti-coagulants) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, the centrifugal 15min of 2000r/min after blood clotting, gets serum and send clinical laboratory of affiliated hospital of Beijing Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, hepatic glycogen detection kit detects Mouse Liver glycogen content.

Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument assay method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(blood lactase acid TG-AUC=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)

7. observation index walking weight load, blood lactase acid, urea, glycogen initial value

8. statistical method experimental data is used represent, employing t inspection is compared between organizing

9. experimental result

9.1 the present invention contain the pulp food of probio to the impact of Mouse Weight

Each group of mouse is after giving G1 ~ G7 medicine, before, in, shown in post-weight sees the following form respectively, the original body mass of each group mouse and increase weight body weight and the more equal no difference of science of statistics of control group (P > 0.05), show G1 ~ G7 medicine all without obvious toxicity.Experimental result refers to table 6.

The original body mass of table 6 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight

9.2 the present invention contain the pulp food of probio to the impact of mice burden swimming time

After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), the present invention compares with blank group containing pulp food G3 ~ G7 medicine of probio, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 medicine.The results detailed in Table 7.

Table 7 contains the pulp food of probio to the impact of mice burden swimming time

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.3 the present invention contain the pulp food of probio to the impact of blood lactase acid before and after mouse movement

It is of the present invention containing after the pulp food of probio that per os gives mouse, the present invention to compare with control group blood lactase acid TG-AUC after mouse movement containing pulp food G3 ~ G7 medicine of probio significant difference (P < 0.05), decrease though G1 ~ G2 medicine group Mouse Blood lactic acid TG-AUC compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 8.

Table 8 the present invention contains the pulp food of probio to the impact of blood lactase acid level before and after mouse movement

" * " p<0.05vs blank;

9.4 the present invention contain the pulp food of probio to the impact of Mouse Liver glycogen

After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), the present invention compares with blank group containing pulp food G3 ~ G7 medicine of probio, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 9.

Table 9 the present invention contains the pulp food of probio to the impact of Mouse Liver glycogen content

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.5 the present invention contain the pulp food of probio to the impact of mice serum urea

After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), the present invention compares with blank group containing pulp food G3 ~ G7 medicine of probio, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 10.

Table 10 the present invention contains the pulp food of probio to the impact of mice serum urea content

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

10. experiment conclusion

This experiment is mainly through mice burden swimming experiment, and the deposit simultaneously detecting Mouse Liver glycogen observes the pulp food raising immunity of the present invention containing probio, the effect of antifatigue.Preliminary Results shows below:

1, G3 ~ G7 of the present invention all can extend the mice burden swimming time (P < 0.01) containing the pulp food of probio, and successful is better than the pulp food containing probio of other G1 ~ G2.

2, biochemistry detection aspect display, G3 ~ G7 of the present invention all can reduce containing each dosage group of pulp food of probio the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although the pulp food containing probio of other G1 ~ G2 also can reduce the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);

3, G3 ~ G7 of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver containing each dosage group of pulp food of probio, and successful is better than the pulp food containing probio of other G1 ~ G2;

4, high lithemia model finds, G3 ~ G7 of the present invention significantly can reduce the content (P < 0.01) of urea in serum after mouse swimming containing the pulp food of probio, and successful is better than the pulp food of other G1 ~ G2 containing probio;

11. conclusions

Above-mentioned experiment proves that the pulp food that the present invention contains probio can significantly improve immunity of organisms, improve muscle power and the endurance of mouse, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.

Claims

1., containing a pulp food for probio, the raw material primarily of following parts by weight obtains: cow's milk 50-70 part, fruit 30-50 part, sucrose 16-18 part, sprouted unpolished rice 6-9 part, extracts of Chinese herbal medicine 5-7 part, leavening 7-9 part, prebiotic factor 7-9 part, protective agent 6-8 part, stabilizing agent 0.1-0.3 part;

Described prebiotic factor weight consists of: oligoisomaltose 20-30 part, galactooligosaccharide 20-30 part, FOS 15-25 part, xylo-oligosaccharide 15-25 part, stachyose 10-15 part, soyabean oligosaccharides 10-15 part, gossypose 10-15 part, oligomeric maltose 8-12 part.

2. the pulp food containing probio as claimed in claim 1, it is characterized in that, described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 7-9:6-8:2-4:1-3 Homogeneous phase mixing in mass ratio, adding mixed material quality 0.1-1 pH value is doubly that the lactic acid of 3.8-4.5 soaks 3-8h, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, crushed material particle diameter 0.5-3mm, then crushed material quality 10-20 water is doubly added, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high-pressure pulse electric process 20-30min is carried out under pulse frequency 200-300Hz condition, then under power 150-300W condition, carry out microwave irradiation in room temperature and extract 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the mixed enzyme of extract quality 3-5%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis liquid filters, filtrate is concentrated, dry, pulverize and obtain protective agent,

Described mixed enzyme is cellulase, protease, amylase, pectase, tannase 5-7:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.

3. the pulp food containing probio as claimed in claim 1, it is characterized in that, the preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature in the supersonic wave cleaning machine that 0.1-0.3% sodium bicarbonate solution is housed, power 200-400W, frequency 20-30KHz ultrasonic cleaning 5-10min, rinsing, drain, put in 12-15 DEG C of water and soak 6-8h, ventilate once every 10-20min, ventilation pressure 0.10-0.13MPa, simultaneously in electric-field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 100-300Hz carries out high-pressure pulse electric process, until brown rice moisture content is 35-38%, brown rice after immersion drains, in growing floor, carry out dark germination, dark germination temperature keep 20-24 DEG C, the dark germination time is 12-14h, period spray water be 42-44% to brown rice moisture content, then putting temperature is soak 10-15min in the water of 35-45 DEG C to carry out killing embryo, until brown rice moisture content is to 43-45%, brown rice after soaking again carries out dark germination in growing floor, and dark germination temperature keeps 23-25 DEG C, and the dark germination time is 18-22h, brown rice after germinateing again is dry in frequency 2450MHz, power 3000W, temperature 50-60 DEG C, thickness of feed layer 2-4cm condition microwave irradiation, and wherein microwave irradiation 10s, interval 10s, being dried to moisture is 12-14%, packs and obtains sprouted unpolished rice.

4. the pulp food containing probio as claimed in claim 1, is characterized in that, the preparation method of described extracts of Chinese herbal medicine, comprise the steps: to count by weight, take Radix Astragali 15-17 part, cordate houttuynia 15-17 part, barrenwort 13-16 part, hawthorn 12-15 part, honeysuckle 12-15 part, Ligusticum wallichii 8-12 part, root of kudzu vine 6-10 part, Radix Angelicae Sinensis 6-10 part, Radix Codonopsis 6-10 part, sterculia seed 5-8 part, fruit of glossy privet 3-7 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70 DEG C-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter, obtain the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85 DEG C-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, namely Homogeneous phase mixing obtains extracts of Chinese herbal medicine;

5. the pulp food containing probio as claimed in claim 1, it is characterized in that, described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 10-15 part, tremella polysaccharides 6-10 part, gelatin 3-5 part, pectin 3-5 part.

6. the pulp food containing probio as claimed in claim 5, it is characterized in that, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber, common oats fibre 4-6:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric process 10-15min under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;

7. the pulp food containing probio as claimed in claim 1, it is characterized in that, described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 30-40 part, bifidobacterium bifidum 25-35 part, bifidobacterium lactis 15-25 part, streptococcus thermophilus 15-25 part, bifidobacterium longum 15-20 part, bifidobacterium infantis 15-20 part, bifidobacterium adolescentis 13-18 part, lactobacillus bulgaricus 12-15 part, lactobacillus acidophilus 10-15 part, Lactobacillus casei 8-10 part, Lactobacillus rhamnosus 5-8 part.

8. the pulp food containing probio as claimed in claim 7, it is characterized in that, Lactobacillus plantarum pulvis is the pulvis 1-3:4-6 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

9. the preparation method of the pulp food containing probio as described in as arbitrary in claim 1-8, is characterized in that, comprise the steps:

10. as claimed in claim 9 containing the preparation method of the pulp food of probio, it is characterized in that, described fruit is that one or more fruit following form alone or in combination: strawberry, the red certain kind of berries, blueberry, raspberry, Huang Tao, cherry, Kiwi berry, peach, apple, pears, pineapple, banana, lichee, mango, grape, Malus spectabilis, Chinese pear-leaved crabapple, watermelon, "Hami" melon, muskmelon, coconut palm fruit, orange, mulberries.