CN104488706A - Dendrobe officinale transplanting method - Google Patents
Dendrobe officinale transplanting method Download PDFInfo
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- CN104488706A CN104488706A CN201410721857.2A CN201410721857A CN104488706A CN 104488706 A CN104488706 A CN 104488706A CN 201410721857 A CN201410721857 A CN 201410721857A CN 104488706 A CN104488706 A CN 104488706A
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Abstract
The invention discloses a dendrobium officinale transplanting method. The method comprises technical steps of root cultivation, seedling hardening, transplanting and the like. By a strict multiplex comparative test research, the method is proven to have unique innovation in prescription and culture condition of a culture medium in different culture stages, and the constitution and proportion of a transplanting medium; the survival rate of hardened sphagna seedling is 95%; the survival rate of matrix transplanting is 97%. By adopting the method disclosed by the invention to build a technical dendrobium officinale rapid propagation system for artificial propagation, the propagation of the dendrobium officinale can be quickened; by a sphagna seedling hardening stage, the survival rate of the dendrobium officinale can be greatly increased, and the cultured dendrobium officinale can be used as an excellent medicinal material.
Description
Technical field
The present invention relates to a kind of dendrobium candidum Plant Tissue Breeding fast breeding technique, particularly relate to a kind of dendrobium candidum tissue transplanting method.
Background technology
Dendrobium candidum (Dendrobium officinale Kimura et Migo) is under the jurisdiction of orchid (Orchidaceae), Dendrobium (Dendrobium Sw.) the perennial herbaceous plant that grows nonparasitically upon another plant.Dendrobium officinale great majority are distributed in the countries and regions such as East Asia, Southeast Asia and Australia.China dendrobium officinale great majority are distributed in the mountain area on the ground such as Anhui on the south the Qinling Mountains, Huaihe River, Zhejiang, Jiangxi, cloud, expensive, river; primary growth is in the rock crevice of lofty mountains and steep hills overhanging cliff; dendrobium officinale resource is close to exhausted now, is the rare or endangered species of state key second class protection.Containing the healthy medicinal active ingredient that is beneficial to man in a large number in dendrobium candidum, there is unique medical value, there is clearind deficient heat, nourishing Tianjin, tonifying five zang organs consumptive disease, improve immunity and anti-ageing effect of waiting for a long time.Have a wide range of applications in fields such as disease treatment and prevention from suffering from the diseases, nutrition and health care and beautifying face and moistering lotions in recent years.
Summary of the invention
The object of the present invention is to provide a kind of dendrobium candidum tissue transplanting method, the method utilizes the method for sphagna hardening to promote the stalwartness of taking root, promoting dendrobium candidum seedling of dendrobium candidum seedling, greatly improve the survival rate of dendrobium candidum seedling, the growth cycle of dendrobium candidum can be shortened simultaneously.
The present invention is achieved in that a kind of transplanting method of the stem of noble dendrobium, the steps include:
Culture of rootage: Multiple Buds seedling is inoculated into film sealing bottled root media on root induction;
Hardening and transplanting: cultivate 28-30 days on root media after, removing sealed membrane opens bottle hardening 4-5 days, take out plantlet in vitro, in units of by about every clump of 5 strains, clean medium on root and after carrying out disinfection, clockwise the root system of plantlet in vitro is wrapped with the sphagna of sterilization, be transplanted to cultivation in 96 hole dishes;
Sorting: classify by size out 3 grades by tissue-culture container seedling, take different control measures for different grades during domestication;
Sterilizing: adopt liquid medicine to soak seedling root, eliminate germ contamination;
Shine seedling: seedling dewaters, and allows its root feel like jelly;
Transplant: be transplanted in the matrix filling sterilization and cultivate.
Further technical scheme of the present invention is: described in take root
In medium step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
Further technical scheme of the present invention is: in described hardening and transplant step:
To the root sterilization method of plantlet in vitro be: in 0.5% mancozeb solution, dip 5-10s;
To sphagna sterilization method be: be 120 DEG C in temperature, with high steam pot sterilizing 20 minutes;
The formula of described matrix is: pine bark: wood chip=4.5: 1(volume ratio), described matrix 0.5% mancozeb solution and 0.8% Isofenphos methyl soaking disinfection;
Hardening condition is: keep temperature 23-27 DEG C, air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day then waters 1 time for 9-11 days, often secondaryly irrigated sphagna, seedling well developed root system after 2 months;
Cultivation condition is: maintain the temperature at 23-27 DEG C, air humidity 73-78%, and fine day watered 1 time every 2-3 days, and the rainy day then waters 1 time for 7-9 days, often secondaryly irrigates matrix, within 15-17 days, survives.
The invention has the beneficial effects as follows: dendrobium candidum tissue is cultivated and taken root and transplanting method, comprises the sport technique segments such as culture of rootage, hardening and transplanting.By strict Multiple range test experimental study, have original innovation from the composition and proportioning etc. of the formula of the medium of different cultivation stages, condition of culture and transplanting medium, sphagna hardening survival rate is 95%, and substrate culture survival rate is 97%.Application the present invention sets up rapid propagation of Dendrobium candidum technical system, and carry out artificial propagation, the expansion accelerating dendrobium candidum is numerous, can be used as excellent medicinal material.
Embodiment
Dendrobium candidum tissue of the present invention is cultivated and is taken root and transplanting method, its preferably embodiment comprise step:
A transplanting method for the stem of noble dendrobium, the steps include:
Culture of rootage: Multiple Buds seedling is inoculated into film sealing bottled root media on root induction;
Hardening and transplanting: cultivate 28-30 days on root media after, removing sealed membrane opens bottle hardening 4-5 days, take out plantlet in vitro, in units of by about every clump of 5 strains, clean medium on root and after carrying out disinfection, clockwise the root system of plantlet in vitro is wrapped with the sphagna of sterilization, be transplanted to cultivation in 96 hole dishes;
Sorting: classify by size out 3 grades by tissue-culture container seedling, take different control measures for different grades during domestication;
Sterilizing: adopt liquid medicine to soak seedling root, eliminate germ contamination;
Shine seedling: seedling dewaters, and allows its root feel like jelly;
Transplant: be transplanted in the matrix filling sterilization and cultivate.
In described root media step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
In described hardening and transplant step:
To the root sterilization method of plantlet in vitro be: in 0.5% mancozeb solution, dip 5-10s;
To sphagna sterilization method be: be 120 DEG C in temperature, with high steam pot sterilizing 20 minutes;
The formula of described matrix is: pine bark: wood chip=4.5: 1(volume ratio), described matrix 0.5% mancozeb solution and 0.8% Isofenphos methyl soaking disinfection;
Hardening condition is: keep temperature 23-27 DEG C, air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day then waters 1 time for 9-11 days, often secondaryly irrigated sphagna, seedling well developed root system after 2 months;
Cultivation condition is: maintain the temperature at 23-27 DEG C, air humidity 73-78%, and fine day watered 1 time every 2-3 days, and the rainy day then waters 1 time for 7-9 days, often secondaryly irrigates matrix, within 15-17 days, survives.
Claims (3)
1. a transplanting method for the stem of noble dendrobium, is characterized in that: the steps include:
Culture of rootage: Multiple Buds seedling is inoculated into film sealing bottled root media on root induction;
Hardening and transplanting: cultivate 28-30 days on root media after, removing sealed membrane opens bottle hardening 4-5 days, take out plantlet in vitro, in units of by about every clump of 5 strains, clean medium on root and after carrying out disinfection, clockwise the root system of plantlet in vitro is wrapped with the sphagna of sterilization, be transplanted to cultivation in 96 hole dishes;
Sorting: classify by size out 3 grades by tissue-culture container seedling, take different control measures for different grades during domestication;
Sterilizing: adopt liquid medicine to soak seedling root, eliminate germ contamination;
Shine seedling: seedling dewaters, and allows its root feel like jelly;
Transplant: be transplanted in the matrix filling sterilization and cultivate.
2. the transplanting method of a kind of stem of noble dendrobium according to claim 1, is characterized in that: described in take root
In medium step: the formula of described root media is: MS medium+sucrose 30g/L+ agar 8g/L+IBA0.4mg/L+ murphy juice 100g/L, PH5.8-6.0.
3. the transplanting method of a kind of stem of noble dendrobium according to claim 1, is characterized in that: described hardening
With transplant step:
To the root sterilization method of plantlet in vitro be: in 0.5% mancozeb solution, dip 5-10s;
To sphagna sterilization method be: be 120 DEG C in temperature, with high steam pot sterilizing 20 minutes;
The formula of described matrix is: pine bark: wood chip=4.5: 1(volume ratio), described matrix 0.5% mancozeb solution and 0.8% Isofenphos methyl soaking disinfection;
Hardening condition is: keep temperature 23-27 DEG C, air humidity 78-85%, fine day watered 1 time every 4 days, and the rainy day then waters 1 time for 9-11 days, often secondaryly irrigated sphagna, seedling well developed root system after 2 months;
Cultivation condition is: maintain the temperature at 23-27 DEG C, air humidity 73-78%, and fine day watered 1 time every 2-3 days, and the rainy day then waters 1 time for 7-9 days, often secondaryly irrigates matrix, within 15-17 days, survives.
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| CN201410721857.2A CN104488706A (en) | 2014-12-03 | 2014-12-03 | Dendrobe officinale transplanting method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145298A (en) * | 2015-07-30 | 2015-12-16 | 黄旭胜 | Dendrobium officinale pot culturing method |
| CN105557486A (en) * | 2015-12-17 | 2016-05-11 | 福建连天福生物科技有限公司 | Preparation method of light media for dendrobium officinale |
| CN105830893A (en) * | 2016-05-26 | 2016-08-10 | 霍山宝信园石斛开发有限公司 | Raw materials and method for planting Dendrobium candidum |
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| KR100868523B1 (en) * | 2007-01-25 | 2008-11-12 | 김종선 | Mass propagation method through in vitro aseptic germination of Calanthe spp. |
| CN102144541A (en) * | 2010-12-31 | 2011-08-10 | 东莞市睿绅生物技术有限公司 | Three-step method for producing 'dragon head phoenix tail' high-quality officinal dendrobium stem material |
| CN102763596A (en) * | 2012-08-08 | 2012-11-07 | 广东省林业科学研究院 | High-efficiency quick dendrobium candidum tissue culture and propagation method |
| CN103098711A (en) * | 2013-01-28 | 2013-05-15 | 江西金乔园林有限公司 | Rooting and transplanting method for dendrobium officinale tissue culture |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
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2014
- 2014-12-03 CN CN201410721857.2A patent/CN104488706A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100868523B1 (en) * | 2007-01-25 | 2008-11-12 | 김종선 | Mass propagation method through in vitro aseptic germination of Calanthe spp. |
| CN102144541A (en) * | 2010-12-31 | 2011-08-10 | 东莞市睿绅生物技术有限公司 | Three-step method for producing 'dragon head phoenix tail' high-quality officinal dendrobium stem material |
| CN102763596A (en) * | 2012-08-08 | 2012-11-07 | 广东省林业科学研究院 | High-efficiency quick dendrobium candidum tissue culture and propagation method |
| CN103098711A (en) * | 2013-01-28 | 2013-05-15 | 江西金乔园林有限公司 | Rooting and transplanting method for dendrobium officinale tissue culture |
| CN104025987A (en) * | 2014-06-24 | 2014-09-10 | 韶关车八岭农业科技有限公司 | Dendrobium officinale planting method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145298A (en) * | 2015-07-30 | 2015-12-16 | 黄旭胜 | Dendrobium officinale pot culturing method |
| CN105557486A (en) * | 2015-12-17 | 2016-05-11 | 福建连天福生物科技有限公司 | Preparation method of light media for dendrobium officinale |
| CN105830893A (en) * | 2016-05-26 | 2016-08-10 | 霍山宝信园石斛开发有限公司 | Raw materials and method for planting Dendrobium candidum |
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