CN104470607B - 作为亲和配体的掺入有免疫球蛋白片段的蜘蛛丝融合蛋白结构 - Google Patents

作为亲和配体的掺入有免疫球蛋白片段的蜘蛛丝融合蛋白结构 Download PDF

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Publication number
CN104470607B
CN104470607B CN201380035379.3A CN201380035379A CN104470607B CN 104470607 B CN104470607 B CN 104470607B CN 201380035379 A CN201380035379 A CN 201380035379A CN 104470607 B CN104470607 B CN 104470607B
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China
Prior art keywords
protein
fusion protein
organic target
recombination fusion
amino acid
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CN201380035379.3A
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Chinese (zh)
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CN104470607A (zh
Inventor
麦·赫德哈马尔
扬·约翰松
安娜·里辛
P-A·尼格伦
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Immunovia AB
Spiber Technologies AB
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Immunovia AB
Spiber Technologies AB
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN201380035379.3A 2012-05-02 2013-05-02 作为亲和配体的掺入有免疫球蛋白片段的蜘蛛丝融合蛋白结构 Active CN104470607B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166392 2012-05-02
EP12166392.6 2012-05-02
PCT/EP2013/059145 WO2013164404A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands

Publications (2)

Publication Number Publication Date
CN104470607A CN104470607A (zh) 2015-03-25
CN104470607B true CN104470607B (zh) 2017-04-05

Family

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CN201380035379.3A Active CN104470607B (zh) 2012-05-02 2013-05-02 作为亲和配体的掺入有免疫球蛋白片段的蜘蛛丝融合蛋白结构

Country Status (6)

Country Link
US (1) US9856308B2 (OSRAM)
EP (1) EP2844361B1 (OSRAM)
JP (1) JP6302895B2 (OSRAM)
CN (1) CN104470607B (OSRAM)
CA (1) CA2872305A1 (OSRAM)
WO (1) WO2013164404A1 (OSRAM)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102652175B (zh) 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品
CN104877031B (zh) * 2015-05-23 2019-04-23 浙江省农业科学院 一种新型融合蛋白nscr5及其制备方法
WO2017020291A1 (en) * 2015-08-06 2017-02-09 Wuxi Biologics (Shanghai) Co. Ltd. Novel anti-pd-l1 antibodies
JPWO2017188346A1 (ja) * 2016-04-27 2019-02-28 国立大学法人 東京大学 マイクロファイバーを用いた血中循環細胞の捕捉及び回収用材料及び当該材料を用いる方法
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
JPWO2020158947A1 (ja) * 2019-01-31 2021-12-02 Ｓｐｉｂｅｒ株式会社 ポリペプチド
CA3158715A1 (en) * 2019-11-18 2021-05-27 CentricsBio, Inc. Composition for preventing or treating cancer, comprising anti-cd300c monoclonal antibodies
CN117756926B (zh) * 2023-12-26 2024-06-07 广东普言生物科技有限公司 一种重组ⅩⅦ型胶原蛋白Pro.C17及其制备方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1642976A (zh) * 2002-03-25 2005-07-20 阿默森生物科学有限公司 一种突变的免疫球蛋白－结合蛋白
WO2005111068A2 (en) * 2004-04-29 2005-11-24 E.I. Dupont De Nemours And Company A method for purifying and recovering silk proteins using magnetic affinity separation
WO2007078239A2 (en) * 2005-12-30 2007-07-12 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins

Family Cites Families (8)

* Cited by examiner, † Cited by third party
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CA2712304C (en) 1994-06-10 2011-10-25 United States Surgical Corporation Recombinant chimeric proteins and methods of use thereof
JP2000125872A (ja) 1998-09-07 2000-05-09 Terumo Corp 三量体キメラタンパク質およびキメラタンパク質を含有するコラーゲンマトリックス
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
WO2006076711A2 (en) 2005-01-14 2006-07-20 Trustees Of Tufts College Fibrous protein fusions and use thereof in the formation of advanced organic/inorganic composite materials
EP1852470A1 (en) 2006-05-03 2007-11-07 Technische Universität München Multilayer Silk Protein Films
EP2220112B1 (en) * 2007-11-26 2015-08-26 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Compositions comprising fibrous polypeptides and polysaccharides
JP2012518398A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
CA2815267C (en) 2010-10-27 2019-06-25 Spiber Technologies Ab Spider silk fusion protein structures for binding to an organic target

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1642976A (zh) * 2002-03-25 2005-07-20 阿默森生物科学有限公司 一种突变的免疫球蛋白－结合蛋白
WO2005111068A2 (en) * 2004-04-29 2005-11-24 E.I. Dupont De Nemours And Company A method for purifying and recovering silk proteins using magnetic affinity separation
WO2007078239A2 (en) * 2005-12-30 2007-07-12 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Characterization and optimization of RGD-containing silk blends to support osteoblastic differentiation;Morgan et al.;《Biomaterials》;20080305;第29卷(第16期);第2556-2563页 *

Also Published As

Publication number Publication date
WO2013164404A1 (en) 2013-11-07
JP2015518489A (ja) 2015-07-02
JP6302895B2 (ja) 2018-03-28
US9856308B2 (en) 2018-01-02
CN104470607A (zh) 2015-03-25
US20150119554A1 (en) 2015-04-30
CA2872305A1 (en) 2013-11-07
EP2844361A1 (en) 2015-03-11
EP2844361B1 (en) 2020-03-25

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