CN104435625A - Compound traditional Chinese herbal medicine preparation for retarding transport stress of beef cattle and preparation method thereof - Google Patents
Compound traditional Chinese herbal medicine preparation for retarding transport stress of beef cattle and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a compound traditional Chinese herbal medicine preparation for retarding the transport stress of beef cattle and a preparation method thereof. The preparation is prepared from the following raw materials in parts by weight: 8-10 parts of rhodiola rosea, 8-10 parts of cortex acanthopanacis senticosus, 6-8 parts of herba houttuyniae, 4-6 parts of licorice root, 4-6 parts of pogostemon cablin, 3-5 parts of rhizoma atractylodis macrocephalae, 6-8 parts of astragalus membranaceus, 21-23 parts of hawthorn, 5-7 parts of rhizoma anemarrhenae and 5-7 parts of poria cocos. Based on the theories of Chinese veterinary medicine, the compound traditional Chinese herbal medicine preparation is prepared from traditional Chinese medicinal materials serving as raw materials by means of drying, enzyme treatment, extraction and mixing so as to improve the tolerance of beef cattle on a transport stressor in the transporting process, activate and harmonize Qi-blood and reinforce immunity, so that the constitution can be recovered in a short time. According to the method, enzyme treatment and extracting technology are effectively combined, the influence of cell walls is broken away, and the extracting effect is expected to be improved. The traditional Chinese herbal medicine preparation is prepared into a liquid-state medicine, is convenient to dose, and is beneficial to intake and absorption of the medicine.
Description
Technical field
The present invention relates to animal transport technical field, particularly a kind ofly slow down Chinese medicine formula of beef cattle transport stress and preparation method thereof.
Technical background
Beef cattle transport stress syndrome refers to that beef cattle causes Abwehrkraft des Koepers to decline because of the multiple stressor caused by long-distance traffic, causes various cause of disease to be availed oneself of the opportunity to get in, and causes the comprehensive syndrome that respiratory tract, digestive tract and even hologathy rationality are reacted.Its stressor mainly contains the driveing of the upper Herba Plantaginis of transport, Baoding, crowded between cattle in transit, fight, hunger and thirst, noise, phobic anxiety, cold stimulation, thermostimulation, miniclimate impact etc. in car.A lot of raiser is owing to lacking the timely Recovery processing measure before necessary transport after anti-stress process and transport, easily make cattle body generation endocrine dysfunction, acid base imbalance in body, metabolite generation obstacle, hyperpyrexia, cough etc., bring out the generation of infectious disease, time serious, can death be caused, cause great economic loss.
At present pig and poultry are mainly concentrated on for transport stress, the research aspect of Fish, and utilize Integrated TCM to slow down the research of transport stress, also rare report.Currently mainly from the aspect such as endocrine and immunology, animal transport stress to be set forth and study.Common control transport stress measure utilizes the combination drug with calm stable effect to prevent as chlorpromazine, bromo agent add in feedstuff to feed, or face injection pentobarbital sodium injection before transport, controls neuroendocrine.The use of chemicals easily produces drug residue on the one hand, is detrimental to health, and injection can cause the stress such as scaring, fear on the other hand.
Publication number is the Chinese invention patent of CN101190003, provide a kind of Chinese herbal medicine compound additive recovered for beef cattle transport stress, formula wherein comprises Massa Medicata Fermentata, Fructus Crataegi, astragalus polysaccharides, active yeast, nicotiamide, vitamin C, vitamin E, zinc sulfate, potassium chloride, ammonium chloride, magnesium oxide.This additive is used for the convalescent period after transport stress, after namely arriving plant, adds Chinese herbal medicine additive, improve body condition in feedstuff, improves digestive function, improves immunity.This additive can not regulate beef cattle body condition before shipping with in transport, reduces the generation even avoiding stress, only uses as a kind of method remedied.
The Chinese invention patent of publication number CN201310239367, discloses one and slows down feeder cattle transport stress briquette feed and preparation method thereof.This feedstuff briquetting comprises green hay, straw, feeder cattle beneficiated burden material complemental feed, premix material.Have that volume is little, comprehensive nutrition, portable feature, effectively solve the forage grass and feed problem of feeder cattle in long-distance transport, but this feedstuff is not recognized, feeder cattle can produce anorexia phenomenon after receiving and stimulating, and can affect the space that feedstuff plays significantly.
Summary of the invention
The object of the invention is to the deficiency for traditional beef cattle transport stress medicine, there is provided a kind of and slow down Chinese medicine formula of beef cattle transport stress and preparation method thereof, the present invention is theoretical according to Chinese veterinarian, is raw material, through super-dry with Chinese medicine, ferment treatment, extract and obtain a kind of Chinese medicine formula after fusion, improving beef cattle to tolerance former to transport stress in transportation, adjust smooth QI and blood, enhancing immunity, so that recover body constitution in the short time.
Another object of the present invention is that just medical material carries out ferment treatment, destroys the compact texture of cell wall, accelerates the stripping of effective medicinal components.Improve the extraction ratio of effective ingredient.
Chinese herbal and crude drugs preparations is made liquid medicine by a further object of the invention, conveniently throws something and feeds, and also contributes to absorption and the absorption of medicine.
Technical scheme provided by the invention is:
A kind of Chinese medicine formula slowing down beef cattle transport stress, wherein, the raw material of following components by weight percent is comprised described in: Radix Rhodiolae 8-10, Cortex Acanthopanacis Radicis 8-10, Herba Houttuyniae 6-8, Radix Glycyrrhizae 4-6, Herba Pogostemonis 4-6, Rhizoma Atractylodis Macrocephalae 3-5, Radix Astragali 6-8, Fructus Crataegi 21-23, Rhizoma Anemarrhenae 5-7 and Poria 5-7.
Preferably, described slows down in the Chinese medicine formula of beef cattle transport stress, also comprises the additive be made up of following component in described Chinese herbal and crude drugs preparations: electrolysis multidimensional 5-7, sodium sorbate 0.1-1 and tween 80 0.1-1.
Slow down a preparation method for the Chinese medicine formula of beef cattle transport stress, wherein:
Enzyme treatment technique and purification technique are effectively combined the elite extracting medical material, finally the elite of gained medical material and described additive are mixed to get Chinese herbal and crude drugs preparations of the present invention, comprise following steps:
Step one, by Radix Rhodiolae, Cortex Acanthopanacis Radicis, the Radix Astragali, Fructus Crataegi, Radix Glycyrrhizae, Poria and the Rhizoma Anemarrhenae mixing after with cellulase with 150-180: 1 ratio mix, 35-55 DEG C of stirring reaction 2-8 hour, mix with 7-9 times amount 70-80% ethanol again, then heating and refluxing extraction 3 times, each 2h obtains alcohol extract, filter to obtain alcohol extract, reclaim ethanol, after medicinal liquid evaporate to dryness, obtain ointment;
Step 2, by Herba Houttuyniae, Herba Pogostemonis, the Rhizoma Atractylodis Macrocephalae respectively with cellulase with 380-420: 1 ratio mix, add 2-3 times of distilled water and stir 3-4 hour at 40-45 DEG C, re-use steam distillation and extract quintessence oil, remaining part decocts, and collects the medicinal liquid obtained;
Step 3, gained ointment, gained quintessence oil and gained medicinal liquid are mixed to get described medical material elite;
Step 4, namely described medicine essence and tween 80, sodium sorbate and electrolysis Multidimensional and Hybrid are obtained described Chinese herbal and crude drugs preparations.
Preferably, in the preparation method of described Chinese medicine formula, each component of described Chinese herbal and crude drugs preparations was carried out drying before extraction, is dried to constant weight at 60-70 DEG C.
Preferably, in the preparation method of described Chinese medicine formula, after each component drying of described Chinese herbal and crude drugs preparations, be prepared as the coarse powder that particle diameter is 0.1-10mm, and using described coarse powder as extraction material.
Preferably, in the preparation method of described Chinese medicine formula, described alcohol extract obtains ointment after Distillation recovery ethanol.
Preferably, in the preparation method of described Chinese medicine formula, after described extraction of essential oil, container closure is used to preserve.
The present invention has following beneficial effect: first, the present invention is theoretical according to Chinese veterinarian, take Chinese medicine as raw material, through super-dry, extract and obtain after merging a kind of environmental protection, safe without toxic side effect and drug residue free for slowing down this Chinese herbal and crude drugs preparations of Chinese medicine formula of beef cattle transport stress for improving beef cattle to tolerance former to transport stress in transportation, adjust smooth QI and blood, enhancing immunity, so that recover body constitution in the short time.
Secondly, the present invention processes first using cellulase medical material, extracts after abolishing the restriction of cell wall to medicine again, improves the extraction ratio of medicine essence, improves the nutritive value of medicament of the present invention, avoid causing medical material to waste.
Again, the present invention is according to beef cattle in transportation, and water loss speeds and increases, and the feature of easy thirsty, makes liquid medicine by Chinese herbal and crude drugs preparations, contribute to absorption and the absorption of medicine.Herbal mediciment of the present invention is easy to carry, and before beef cattle transport, in transit, adds in drinking-water and take after transport.
Finally, reasonable recipe of the present invention, compatibility is proper, the timid heresy of righting, treating both the principal and secondary aspects of a disease, and described preparation method uses heating and refluxing extraction Fa Gengyi to promote the extraction completely of effective ingredient in Chinese herbal medicine.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is elaborated, can implement according to this after consulting this description to make those of ordinary skill in the art.
Slow down a Chinese medicine formula for beef cattle transport stress, wherein, described in comprise the raw material of following components by weight percent: Radix Rhodiolae 9 parts, Cortex Acanthopanacis Radicis 9 parts, the Radix Astragali 7 parts, Herba Houttuyniae 7 parts, Fructus Crataegi 11 parts, 5 parts, Radix Glycyrrhizae, Herba Pogostemonis 5 parts, the Rhizoma Atractylodis Macrocephalae 4 parts, 6 parts, Poria, the Rhizoma Anemarrhenae 7 parts.
Described slows down in the Chinese medicine formula of beef cattle transport stress, also comprises the additive be made up of following component in described Chinese herbal and crude drugs preparations: electrolysis multidimensional 6 parts, sodium sorbate 0.5 part, tween 80 0.6 part.
In above-mentioned raw material, described Cortex Acanthopanacis Radicis has replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, resisting fatigue, neuroprotective unit, immunomodulating, the adaptive effect of enhancing.
Described Radix Rhodiolae returns lung meridian, has QI invigorating lung heat clearing, anti-fatigue anoxia-tolerance, Fructus Alpiniae Oxyphyllae are nourished heart, effect of enhancing immunity.
The described Radix Astragali have protect the liver, diuresis, anti-stress, blood vessel dilating, enhancing immunity effect.
Described Herba Houttuyniae has the effects such as heat-clearing and toxic substances removing, promoting urination to remove dampness, heat clearing away solution dysentery, detumescence treatment skin ulcer.
Described Fructus Crataegi has the effects such as spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting.
Described Radix Glycyrrhizae has the effects such as heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen.
Described Herba Pogostemonis returns spleen, stomach, lung three warp, and have eliminating turbid pathogen with aromatics, the preventing or arresting vomiting that whets the appetite, deliver the function of expelling summer-heat, for turbid damp obstructing in middle-JIAO, gastral cavity painful abdominal mass is vomitted, heat-damp in summer asthenia, does not uncomfortable in chestly relax, and cold-damp closes summer-heat, and stomachache is vomited and diarrhoea, the diseases such as nasosinusitis headache.
Effect of the invigorating the spleen and benefiting QI of described Rhizoma Atractylodis Macrocephalae tool, dampness diuretic, hidroschesis.Be mainly used in insufficiency of the spleen lack of appetite, abdominal distention had loose bowels, the disease such as phlegm retention vertigo and palpitation, edema, spontaneous perspiration.
Described Poria has the function of promoting diuresis to eliminate damp pathogen, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.
The described Rhizoma Anemarrhenae has nourishing YIN to lower pathogenic fire, moisturizes laxation, the effect of sharp defecation.
Described electrolysis multidimensional is rich in multivitamin and trace element, corrects every Serum Indexes in time.
Slow down a preparation method for the Chinese medicine formula of beef cattle transport stress, wherein:
Enzyme treatment technique and purification technique are effectively combined the elite extracting medical material, finally the elite of gained medical material and described additive are mixed to get Chinese herbal and crude drugs preparations of the present invention, comprise following steps:
Step one, by the Radix Rhodiolae in the dry medical material of gained, Cortex Acanthopanacis Radicis, the Radix Astragali, Fructus Crataegi, Radix Glycyrrhizae, Poria, the Rhizoma Anemarrhenae etc. after ratio breaks into 0.5-10mm coarse powder by weight with cellulase with 170: 1 ratio mix, 50 DEG C of stirring reactions 3 hours, mix with 8 times amount 70-80% ethanol again, then heating and refluxing extraction 4 times, each 1h obtains alcohol extract, obtains ointment by after the separation of ethanol in gained alcohol extract.
Step 2, by Herba Houttuyniae, Herba Pogostemonis, the Rhizoma Atractylodis Macrocephalae respectively with cellulase with 400: 1 ratio mix, add 3 times of distilled water to stir 3 hours at 40 DEG C, re-use steam distillation and extract quintessence oil, after quintessence oil sealing is preserved, after remaining part decocts, filter squeezing and collect the medicinal liquid obtained.
Step 3, by gained ointment and gained medicinal liquid mixing, be constantly stirred to completely dissolve obtain mixed liquor.
Step 4, gained quintessence oil is mixed with surface active agent tween-80 after add gained mixed liquor and mix in the lump, finally add antiseptic sodium sorbate, electrolysis Multidimensional and Hybrid evenly encapsulate afterwards.
In the preparation method of described Chinese medicine formula, herbal raw material 60 DEG C of freeze-day with constant temperature to constant weights in drying baker were obtained dry medical material by each component of described Chinese herbal and crude drugs preparations before extracting.At 60 DEG C, the dry breeding can avoiding common microbiological, can avoid again high temperature to damage medical material.
In the preparation method of described Chinese medicine formula, after each component drying of described Chinese herbal and crude drugs preparations, be prepared as the coarse powder that particle diameter is 0.1-10mm, and using described coarse powder as extraction material.
In the preparation method of described Chinese medicine formula, described alcohol extract obtains ointment after Distillation recovery ethanol.
In the preparation method of described Chinese medicine formula, after described extraction of essential oil, container closure is used to preserve.
The usage of Chinese herbal and crude drugs preparations of the present invention: start shipment the previous day beef cattle and feed water three times, be added to the water in transit and drink.
Embodiment 1
One, experimentation
Transport 12 Simmental beefs from Guangdong to Hengxian County, the vehicles are automobile.Be divided into experimental group and matched group at random, often organize 6, matched group is only drunk to water purification before starting shipment and in way, does not add any medicine.Experimental group before preparation is started shipment 3 little constantly, with the addition of compound Chinese medicinal preparation of the present invention in way, add by the every 300KG body weight of 200ML medicinal liquid.
Two, Indexs measure
Disposal vacuum blood taking tube is adopted to carry out venous blood collection.Blood sample respectively with start shipment 5 hours before, transport after 5 hours, transport arrive time take, every the 3-5ML that at every turn takes a blood sample, separation of serum, is placed in refrigerator.Send to hospital at once after arrival and carry out following Indexs measure.
Three, characteristic evidences
Hydrocortisone (Cor): animal under the stimulation of stressor in blood Determination of cortisol can there is significant change, hydrocortisone is considered to the objective indicator evaluating Animal stress degree.
Thyroliberin (ACTH): under stress state, the function of hypothalamo pituitary-adrenal cortex axle (HPA) strengthens, the also increase in various degree of hydrocortisone (Cor) and thyroliberin (ACTH) level, and then inhibitory action is presented to nearly all immunocytes such as lymphocyte, neutrophilic granulocyte, macrophages, the generation of immune cell propagation, cytokine and antibody is regulated and controled, thus affects the immunologic function of animal.
Creatine kinase (CK): its active significantly rising is the indicator of muscle cell membrane system damage, and transport stress makes myocyte impaired, and CK content in serum also can be made to raise.
Lactic acid dehydrogenase (LDH): lactic acid dehydrogenase is containing zinc glycolytic ferment is the index of reflection conscience acute injury.When the content of LDH in serum raises, illustrate that transport stress makes myocyte impaired.Also have, in transportation, total protein (TP), albumin (ALB), glutamic oxaloacetic transaminase, GOT (AST) and glucose (GLU) activity all have rising in various degree, and they also can as the evaluation index of Animal stress degree.
Experimental result:
Table 1 Chinese herbal and crude drugs preparations of the present invention is on the impact of beef cattle Hormone traits
(note: * represents significant difference (P < 0.05); * represents pole significant difference (P < 0.01)
Found out by table 1, experimental group is compared with matched group, and hydrocortisone (Cor) and thyroliberin (ACTH), after starting shipment 5h and when transporting to, reduce in remarkable respectively.Illustrate that gained Chinese herbal and crude drugs preparations of the present invention has the effect slowing down hydrocortisone (Cor) and thyroliberin (ACTH) and raise.
Table 2 Chinese herbal and crude drugs preparations of the present invention is on the impact of beef cattle biochemical indicator
(note: compare between group: * represents significant difference, * * represents that difference is extremely remarkable; Compare in group: compared with before administration, alphabetical identical table differential is different not remarkable; Letter is different represents significant difference)
As can be seen from Table 2, compared with before administration, matched group is after starting shipment 5h and when transporting to, and each index all significantly raises; And in experimental group except total protein (TP) and lactic acid dehydrogenase (LDH), other indexs also all significantly raise.But experimental group is compared with matched group, and except albumin (ALB), other indexs, after starting shipment 5h and when arriving, all reduce in remarkable.Show that Chinese herbal and crude drugs preparations of the present invention has certain anti-stress effect.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the general concept that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (7)
1. one kind is slowed down the Chinese medicine formula of beef cattle transport stress, it is characterized in that, described in comprise the raw material of following components by weight percent: Radix Rhodiolae 8-10, Cortex Acanthopanacis Radicis 8-10, Herba Houttuyniae 6-8, Radix Glycyrrhizae 4-6, Herba Pogostemonis 4-6, Rhizoma Atractylodis Macrocephalae 3-5, Radix Astragali 6-8, Fructus Crataegi 21-23, Rhizoma Anemarrhenae 5-7 and Poria 5-7.
2. slow down the Chinese medicine formula of beef cattle transport stress as claimed in claim 1, it is characterized in that, in described Chinese herbal and crude drugs preparations, also comprise the additive be made up of following component: electrolysis multidimensional 5-7, sodium sorbate 0.1-1 and tween 80 0.1-1.
3. slow down a preparation method for the Chinese medicine formula of beef cattle transport stress as claimed in claim 1, it is characterized in that:
Enzyme treatment technique and purification technique are effectively combined the elite extracting medical material, finally the elite of gained medical material and described additive are mixed to get Chinese herbal and crude drugs preparations of the present invention, comprise following steps:
Step one, by Radix Rhodiolae, Cortex Acanthopanacis Radicis, the Radix Astragali, Fructus Crataegi, Radix Glycyrrhizae, Poria and the Rhizoma Anemarrhenae mixing after with cellulase with 150-180: 1 ratio mix, 35-55 DEG C of stirring reaction 2-8 hour, mix with 7-9 times amount 70-80% ethanol again, then heating and refluxing extraction 3-5 time, each 1-2h obtains alcohol extract, filter to obtain alcohol extract, reclaim ethanol, after medicinal liquid evaporate to dryness, obtain ointment;
Step 2, by Herba Houttuyniae, Herba Pogostemonis, the Rhizoma Atractylodis Macrocephalae respectively with cellulase with 380-420: 1 ratio mix, add 2-3 times of distilled water and stir 3-4 hour at 40-45 DEG C, re-use steam distillation and extract quintessence oil, remaining part decocts, and collects the medicinal liquid obtained;
Step 3, gained ointment, gained quintessence oil and gained medicinal liquid are mixed to get described medical material elite;
Step 4, namely described medicine essence and tween 80, sodium sorbate and electrolysis Multidimensional and Hybrid are obtained described Chinese herbal and crude drugs preparations.
4. the preparation method of Chinese medicine formula as claimed in claim 3, is characterized in that, each component of described Chinese herbal and crude drugs preparations was carried out drying before extraction, is dried to constant weight at 60-70 DEG C.
5. the preparation method of Chinese medicine formula as claimed in claim 4, is characterized in that, be prepared as the coarse powder that particle diameter is 0.1-10mm after each component drying of described Chinese herbal and crude drugs preparations, and using described coarse powder as extraction material.
6. the preparation method of Chinese medicine formula as claimed in claim 3, it is characterized in that, described alcohol extract obtains ointment after Distillation recovery ethanol.
7. the preparation method of Chinese medicine formula as claimed in claim 3, is characterized in that, use container closure to preserve after described extraction of essential oil.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105248854A (en) * | 2015-11-06 | 2016-01-20 | 安徽省田丰牧业科技有限公司 | Long-distance transport stress reaction preparation for ruminants and using method thereof |
| CN113616689A (en) * | 2021-08-23 | 2021-11-09 | 四川农业大学 | Acanthopanax root compound composition and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559095A (en) * | 2009-06-02 | 2009-10-21 | 河南新正好生物工程有限公司 | Veterinary compound acanthopanax granules with immunopotentiation and preparation method thereof |
| KR20130104692A (en) * | 2012-03-15 | 2013-09-25 | 김형준 | Composition foranti-obesity using extract of crataegi fructus |
| CN104173628A (en) * | 2014-08-11 | 2014-12-03 | 宾阳县王灵镇良骏养鹅场 | Traditional Chinese medicine composition for resisting pig immunosuppression |
-
2014
- 2014-12-09 CN CN201410745634.XA patent/CN104435625B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559095A (en) * | 2009-06-02 | 2009-10-21 | 河南新正好生物工程有限公司 | Veterinary compound acanthopanax granules with immunopotentiation and preparation method thereof |
| KR20130104692A (en) * | 2012-03-15 | 2013-09-25 | 김형준 | Composition foranti-obesity using extract of crataegi fructus |
| CN104173628A (en) * | 2014-08-11 | 2014-12-03 | 宾阳县王灵镇良骏养鹅场 | Traditional Chinese medicine composition for resisting pig immunosuppression |
Non-Patent Citations (1)
| Title |
|---|
| 秦葵等: "归脾汤对小鼠抗应激作用的实验研究", 《中成药》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105248854A (en) * | 2015-11-06 | 2016-01-20 | 安徽省田丰牧业科技有限公司 | Long-distance transport stress reaction preparation for ruminants and using method thereof |
| CN113616689A (en) * | 2021-08-23 | 2021-11-09 | 四川农业大学 | Acanthopanax root compound composition and application thereof |
| CN113616689B (en) * | 2021-08-23 | 2022-03-01 | 四川农业大学 | Acanthopanax root compound composition and application thereof |
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