CN113616689B - Acanthopanax root compound composition and application thereof - Google Patents
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Abstract
The invention provides an acanthopanax compound composition and application thereof, wherein the composition comprises the following components in parts by weight: 4-6 parts of acanthopanax, 0.05-0.1 part of patchouli oil and 1-3 parts of poria cocos. The invention also provides acanthopanax compound granules, which comprise the following active ingredients in parts by weight: 4-6 g/kg of acanthopanax, 0.05-0.1 g/kg of patchouli oil, 1-3 g/kg of poria cocos, 0.1-0.3 mg/kg of chromium picolinate, 30-50 g/kg of glucose and 0.1-0.2 g/kg of potassium citrate. The acanthopanax senticosus compound granules are applied to relieving animal transportation stress. Animal experiments show that the proper addition of the compound acanthopanax particles in daily ration can promote the growth performance of the black-bone chicken, improve the metabolic level and the antioxidant function and relieve the influence of transportation stress on the black-bone chicken.
Description
Technical Field
The invention relates to a traditional Chinese medicine compound composition, in particular to an acanthopanax compound composition and application thereof.
Background
Stress is a responsive reaction caused when the animal body is stimulated by all adverse factors, namely stress syndrome. Many external factors can cause stress, such as transportation, excessive heat and cold, lack of nutrients, gas, mental stress, etc., of which transportation is one of the most important stressors. At present, most of livestock and poultry breeding modes are intensive breeding, and the intensive breeding needs transportation before entering and exiting a farm, turning a group and intensively slaughtering, so that transportation stress is inevitably generated. Transportation stress refers to adaptive response and defensive response of the body under the action of various factors such as environmental change in the transportation process, water and food deprivation, bumpiness in the transportation process, artificial pressure and the like, and sub-health, diseases and even death occur when the body cannot resist the influence caused by stress. Currently, economic losses caused by livestock and poultry diseases, disabilities or deaths due to transport stress are huge.
Sedative, electrolyte and amino acid are used as main treatment modes for relieving transportation stress, but the problems of large side effect, drug residue, drug resistance and the like of chemically synthesized drugs greatly influence the health of animals and the safety of livestock and poultry products, and the traditional Chinese medicine prescription preparation has the characteristics of low residue, low side effect, no pollution, no drug-induced diseases, low price and easy obtainment of products. Therefore, the search for suitable traditional Chinese medicine anti-stress drugs can not only ensure the health of livestock and poultry, but also have very important significance for developing the traditional Chinese medicine in China.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an acanthopanax compound composition and application thereof. The technical scheme of the invention is as follows:
in a first aspect, the invention provides an acanthopanax compound composition, which comprises the following components in parts by weight: 4-6 parts of acanthopanax, 0.05-0.1 part of patchouli oil and 1-3 parts of poria cocos.
Preferably, the composition also comprises 0.03-0.07 part of vitamin C and 0.03-0.07 part of vitamin E.
In a second aspect, the invention provides the application of the composition in preparing an anti-stress medicament for animals.
In a third aspect, the invention provides an acanthopanax compound granule, which comprises the following active ingredients in the granule by weight: 4-6 g/kg of acanthopanax, 0.05-0.1 g/kg of patchouli oil, 1-3 g/kg of poria cocos, 0.1-0.3 mg/kg of chromium picolinate, 30-50 g/kg of glucose and 0.1-0.2 g/kg of potassium citrate.
Preferably, the particle further comprises: 30-70 mg/kg of vitamin C and 30-70 mg/kg of vitamin E.
In a fourth aspect, the invention provides a preparation method of the acanthopanax compound granules, which comprises the following steps:
(1) preparing materials according to the weight content of the effective components of the acanthopanax compound particles in the particles;
(2) soaking radix Acanthopanacis Senticosi and Poria with water, adding 40% (v/v) ethanol solution, and extracting for several times to obtain extractive solution, and concentrating to obtain extract;
(3) mixing the extract with oleum herba Pogostemonis and glucose, adding the rest materials, mixing, granulating by wet method, and oven drying.
In a fifth aspect, the invention provides application of the acanthopanax senticosus compound particles in relieving animal transportation stress.
Further, the application method comprises the following steps: adding the compound acanthopanax particles into animal feed according to the amount of 300-500 mg/kg before transportation, and feeding for at least 21 days.
Preferably, the compound acanthopanax particles are added to animal feed in an amount of 500mg/kg before transportation.
Further, the animal is a black-bone chicken.
The invention has the beneficial effects that:
the acanthopanax root, the patchouli oil and the poria cocos are adopted to prepare the traditional Chinese medicine composition and are applied to relieving animal transportation stress, wherein the acanthopanax root is rich in various nutrient substances such as organic acid, amino acid and trace elements and bioactive substances such as polysaccharide and flavone, and has various pharmacological actions such as immunity enhancement, oxidation resistance, inflammation resistance, fatigue resistance, tumor resistance and the like; herba Agastaches has effects of eliminating dampness, relieving summer-heat, and relieving vomit; poria has tranquilizing and heart tonifying effects. Animal experiments show that the proper addition of the compound acanthopanax particles in daily ration can promote the growth performance of the black-bone chicken, improve the metabolic level and the antioxidant function and relieve the influence of transportation stress on the black-bone chicken. Therefore, the compound preparation provides some references for resisting transport stress of animal clinic.
Detailed Description
The total antioxidant capacity (T-AOC), Total Protein (TP), total superoxide dismutase (T-SOD), Glutathione (GSH) and Malondialdehyde (MDA) determination kits in the embodiment of the invention are purchased from Nanjing to build an engineering biological research institute.
The main instruments and equipment in the implementation of the invention are as follows: YP202N electronic balance (Shanghai Qinghai instruments Co., Ltd.), BC-2800Vet full-automatic hemocyte analyzer (Shenzhen Merrill biomedical electronics Co., Ltd.), DNM-9602G enzyme-labeled analyzer (Beijing Pulangen technology Co., Ltd.), RM2235 paraffin slicer (Leica microsystems Co., Ltd.), CX22 optical microscope (Japan Olympus), and the like.
In the description of the present invention, it is to be noted that those who do not specify specific conditions in the examples are performed according to conventional conditions or conditions recommended by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The present invention will now be described in further detail with reference to specific examples, which are intended to be illustrative, but not limiting, of the invention.
Example 1
The embodiment provides an acanthopanax compound composition, which comprises the following components in parts by weight: 4-6 parts of acanthopanax, 0.05-0.1 part of patchouli oil and 1-3 parts of poria cocos. Most of the traditional Chinese medicine formula components applied to the livestock feed are very complex, so that the prepared traditional Chinese medicine has very strange taste, and the traditional Chinese medicine has serious palatability problem when being applied to the livestock feed additive, and can not solve the practical problem. The composition disclosed by the invention is only compounded by three traditional Chinese medicine components and is applied to preparation of an animal anti-stress medicine. In addition, the composition preferably also comprises 0.03-0.07 part of vitamin C and 0.03-0.07 part of vitamin E.
Example 2
The embodiment provides a composition of acanthopanax compound particles, which comprises the following components in parts by weight: acanthopanax senticosus (5g/kg), patchouli oil (0.096g/kg), poria cocos (1g/kg), chromium picolinate (0.2mg/kg), vitamin C (50mg/kg), vitamin E (50mg/kg), glucose (30-50 g/kg) and potassium citrate (0.17 g/kg). The preparation method comprises the following steps:
(1) preparing materials according to the weight content of the effective components of the acanthopanax compound particles in the particles;
(2) soaking radix Acanthopanacis Senticosi and Poria with water, adding 40% (v/v) ethanol solution, and extracting for several times to obtain extractive solution, and concentrating to obtain extract;
(3) mixing the extract with oleum herba Pogostemonis and glucose, adding the rest materials, mixing, granulating by wet method, and oven drying.
Example 2
The compound acanthopanax particles in example 2 are used for the investigation of animal transportation stress, and the specific process is as follows:
1. test method
1.1 test animal feeding and management
240 healthy, similarly weighted 1 day old black-bone chicken chicks (46 ± 0.5g) (provided by the agricultural development ltd, foison rock herd, Sichuan). The net rack is used for conventional breeding, the breeding temperature is 35 +/-2 ℃, and the relative humidity is 50-60%.
1.2 test grouping and transport handling
The test design adopts a completely random design and a single-factor control test. 240 1d Tianfu black-bone chickens were selected and randomly divided into 4 groups of 60 chickens each, 4 replicates each, and 15 replicates each. Feeding basal diet for blank control group; feeding low-dose group with feed containing compound radix Acanthopanacis Senticosi granule added at 300 mg/kg; feeding the medium-dose group with a feed of compound acanthopanax granules added according to 400 mg/kg; the high-dose group was fed with a diet (shown in table 1) containing 500mg/kg of the compound acanthopanax particles, the total amount of the experiment was 21 days, and the composition and nutritional level of the basic diet are shown in table 2.
Trial 21d, groups randomly picked 16 chickens loaded in transfer cages (750mm × 550mm × 270mm), each cage containing 8 chickens. In autumn, the average outdoor temperature is 18 ℃ in the test day, the average temperature in the vehicle is 20 ℃ in the transportation process, and the air quality is excellent in the day. The poultry transport vehicle carries out road transport for 2 hours on a road (comprising a traffic light intersection and a bumpy road section) at the speed of about 80km/h, wherein the period comprises slow stop, quick stop, starting, turning and the like, and the process is as close as possible to the road transport process in production practice. Immediately after the transport was finished, the samples were dissected.
TABLE 1 grouping and administration of test animals
TABLE 2 basic diet composition and nutritional levels (air-dried basis)
Note: 1. the premix is fed per kilogramThe grains provide VA 10000IU and vitamin D3 2500IU、VE 0.025g、VK30.005g、VB1 0.0025g、VB20.0075g、VB6 0.005g、VB120.025mg, 0.05g of nicotinic acid, 0.015g of D-pantothenic acid, 0.0015g of folic acid, 0.1375mg of biotin, 0.0064g of Cu, 0.09g of Fe, 0.07g of Zn, 0.106g of Mn, 0.8mg of I and 0.3mg of Se; 2. the nutrient levels are calculated values.
Trial 21d, groups randomly picked 16 chickens loaded in transfer cages (750mm × 550mm × 270mm), each cage containing 8 chickens. In autumn, the average outdoor temperature is 18 ℃ in the test day, the average temperature in the vehicle is 20 ℃ in the transportation process, and the air quality is excellent in the day. The poultry transport vehicle carries out road transport for 2 hours on a road (comprising a traffic light intersection and a bumpy road section) at the speed of about 80km/h, wherein the period comprises slow stop, quick stop, starting, turning and the like, and the process is as close as possible to the road transport process in production practice. Immediately after the transport was finished, the samples were dissected.
1.3 measurement of growth Performance
The health condition, the growth condition and the death condition of the chickens are observed every day, and the feed is settled in time so as to eliminate the feed consumption of the dead chickens. Weighing the chickens 1, 7, 14 and 21d before 09:00 each time in a fasting state by using a repeating unit, and counting the average weight, the average daily feed intake, the average daily gain and the feed weight ratio of the test chickens at each stage.
Average body weight (BW, g) total body weight/number of chickens tested;
average daily gain (ADG, g) (ad/(day of experiment × number of chickens tested));
average daily feed intake (ADFI, g) is feed consumption/(test days × test chickens);
feed weight ratio (F/G) is feed consumption/weight gain.
1.4 measurement of organ index and body weight Change
At 21d of chicks, 2 chickens were selected each time before and after transportation, 8 chickens in each group were selected, weighed and then killed by intravenous exsanguination, and after the heart, liver, spleen, lung, kidney and filter paper were dried, fat and irrelevant tissues were peeled off and weighed with an electronic balance. And calculating the organ index of the chicken according to the weight of the chicken.
Organ index (g/kg) ═ fresh weight of organ (g) × 100/live weight (g)
All chickens were weighed before and after each replicate to calculate the average change in weight before and after transport.
1.5 sample Collection and processing
When the chickens are 21d away, 2 chickens are repeatedly selected before and after transportation, each group of 8 chickens are subjected to venous blood collection for 2ml, blood is collected into a vacuum collection pipe, standing is carried out for 1h at room temperature, serum is centrifugally collected, and the chicken is stored in a refrigerator at minus 80 ℃ for later use; the animals were sacrificed after blood sampling, the liver tissue was quickly separated and taken out and frozen with liquid nitrogen and stored in a refrigerator at-80 ℃ for future use.
1.6 serum Biochemical index detection
Taking out the serum sample stored in a refrigerator at-80 ℃, measuring the content of calcium (Ca) in the serum by a microplate method, measuring the content of phosphorus (P) in the serum by a phosphomolybdic acid method, and measuring the content of glucose (Glu) in the serum by a glucose oxidation method.
1.7 liver antioxidant index detection
Taking out liver tissue preserved in-80 deg.C refrigerator, grinding on ice box, and sampling to detect indexes such as reduced Glutathione (GSH), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), and Malondialdehyde (MDA) in liver. Pathological changes of liver tissue were assessed using HE staining.
1.8 statistical treatment
The data were initially processed using Excel 2010, and multiple comparative analyses were performed using SPSS23.0 software, with the results expressed as "mean ± standard deviation" and P <0.05 as significance of difference.
2. Test results
2.1 Effect of Compound Acanthopanax senticosus granule on growth of Black-bone Chicken
As can be seen from Table 3, the average body weight and average daily gain of the blank group and each test group were not significantly different (P > 0.05). Compared with the blank group, the average daily food consumption of each test group is obviously reduced (P is less than 0.05) at 1-7 d and 1-21 d; the average daily food intake of the low dose group is obviously reduced (P is less than 0.05) at 8-14 d and 15-21 d. Compared with a blank group, the material weight ratio of each test group is remarkably reduced (P is less than 0.05) within 1-7 days; when the time is 1-21 d, the material weight ratio of each test group is reduced (P is more than 0.05).
TABLE 3 influence of Compound radix Acanthopanacis Senticosi granule on growth performance of gallus Domesticus
Note: the difference of the same letter in the same row is not more than 0.05 obviously, and the difference of different letters is less than 0.05 obviously, and the same is as below.
2.2 Effect of Compound Acanthopanax senticosus granule on viscera index and weight change of gallus Domesticus before and after transportation
As can be seen from Table 4, the mean body weights of the blank group and the low-dose group after transportation were significantly reduced (P < 0.05) compared to those of the blank group before transportation, while the mean body weights of the medium-dose group and the high-dose group did not significantly change before and after transportation (P > 0.05). The variation of cardiac index was not significant (P > 0.05) between groups before and after delivery. The liver index changes of each group were not significantly different before and after transport (P > 0.05). Spleen index changes were not significantly different for each group before and after transport (P > 0.05). Before and after transportation, the change of the lung index of each group is not obvious (P is more than 0.05). The test result shows that the compound acanthopanax particles have little influence on viscera indexes of black-bone chickens before and after transportation.
TABLE 4 influence of FUFANGJIRONGJI granule on viscera index of gallus Domesticus before and after transportation
Note: the difference of the same letter in the same row is not more than 0.05 obviously, and the difference of different letters is less than 0.05 obviously, and the same is as below.
2.3 Effect of Compound Acanthopanax senticosus granule on biochemical index of black-bone chicken serum before and after transportation
As can be seen from Table 5, transport stress caused a blank group of serum Ca2+The concentration is obviously reduced (P is less than 0.05); serum Ca of low, medium and high dose group after transportation compared with blank group2+The concentration decreased and the low dose group varied significantly (P < 0.05). The compound acanthopanax particles are beneficial to relieving the serum Ca caused by transport stress2+The change in concentration. Transport stress causes significant reduction in serum GLU content (P < 0.05); the serum GLU levels in the post-transit low dose groups were significantly elevated compared to the blank group (P < 0.05). The compound acanthopanax particles are beneficial to relieving the change of serum GLU content caused by transport stress. The P content of the serum of each group is not changed greatly before and after transportation (P is more than 0.05). Transport stress caused a significant increase in serum LDH levels in the blank (P < 0.05), and the low, medium and high dose groups after transport did not significantly decrease serum LDH levels in the blank (P > 0.05).
TABLE 5 Effect of Compound radix Acanthopanacis Senticosi granule on biochemical indexes of gallus Domesticus serum before and after transportation
2.4 Effect of Compound radix Acanthopanacis Senticosi granule on liver antioxidant index of gallus Domesticus before and after transportation
As can be seen from Table 6, transport stress caused a significant decrease in hepatic GSH levels in the blank group (P < 0.05). Compared with the blank group after transportation, the liver GSH content of each test group is increased, and the high dose group is obviously increased by 6.40% (P is less than 0.05). The compound acanthopanax particles can relieve the reduction of the GSH content of the liver caused by transport stress, and the effect of a high-dose group is optimal. Transport stress caused a significant increase in liver MDA levels in the blank group (P < 0.05). Compared with the blank group after transportation, the liver MDA content of each test group is reduced, and the middle and high dose groups are obviously reduced (P is less than 0.05). The compound acanthopanax particles can relieve the increase of the liver MDA content caused by transportation stress, and the effect of a high-dose group is optimal. Transport stress caused a significant decrease in T-AOC levels in the liver in the blank group (P < 0.05). Compared with the blank group after transportation, the T-AOC content of the liver of each test group is increased, and the T-AOC content of the liver of the middle and high dose groups is obviously increased (P is less than 0.05). The compound acanthopanax particles can relieve the reduction of liver T-AOC level caused by transportation stress, and the effect of a high-dose group is optimal. Transport stress caused a significant decrease in hepatic SOD levels (P < 0.05) in the blank group. Compared with the blank group after transportation, the liver SOD content of each test group is increased, and the liver SOD content of the high-dose group is obviously increased (P is less than 0.05). The compound acanthopanax particles can relieve the decrease of liver SOD content caused by transportation stress, and the effect of a high-dose group is optimal.
TABLE 6 influence of Compound radix Acanthopanacis Senticosi granule on liver oxidation resistance index of gallus Domesticus before and after transportation
In conclusion, the acanthopanax compound particles can reduce the feed weight ratio of black-bone chickens, but have little influence on average daily gain, do not exclude the relation with the variety of the selected chickens and the added dosage, and need to be further researched and determined. The acanthopanax compound particles have no obvious influence on the visceral organ index of the heart, the liver, the spleen, the lung and the kidney of the black-bone chicken, which shows that the acanthopanax compound particles have no obvious negative effect on the black-bone chicken. The research also finds that the transport stress can cause the content of the T-AOC, SOD and GSH in the liver to be reduced, and the content of MDA is increased, namely the transport stress reduces the antioxidant capacity of the liver of the black-bone chicken. The compound acanthopanax particles can relieve oxidative stress caused by transportation stress and keep calcium ions in a stable state. Therefore, the proper addition of the compound acanthopanax particles in the daily ration can promote the growth performance of the black-bone chicken, improve the metabolic level and the antioxidant function and relieve the influence of transport stress on the black-bone chicken.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (2)
1. The acanthopanax compound granules are characterized in that: the weight content of the effective components in the granules is as follows: 4-6 g/kg of acanthopanax, 0.05-0.1 g/kg of patchouli oil, 1-3 g/kg of poria cocos, 0.1-0.3 mg/kg of chromium picolinate, 30-50 g/kg of glucose, 0.1-0.2 g/kg of potassium citrate, 30-70 mg/kg of vitamin C and 30-70 mg/kg of vitamin E.
2. The method for preparing acanthopanax senticosus compound granules of claim 1, which is characterized by comprising the following steps: the method comprises the following steps:
(1) preparing materials according to the weight content of the effective components of the acanthopanax compound particles in the particles;
(2) soaking radix Acanthopanacis Senticosi and Poria with water, adding 40% v/v ethanol solution, and extracting for several times to obtain extractive solution, and concentrating to obtain extract;
(3) mixing the extract with oleum herba Pogostemonis and glucose, adding the rest materials, mixing, granulating by wet method, and oven drying.
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