CN104430096B - A kind of Siniperca chuatsi family selective breeding group isolation hatching method - Google Patents
A kind of Siniperca chuatsi family selective breeding group isolation hatching method Download PDFInfo
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- CN104430096B CN104430096B CN201410764751.0A CN201410764751A CN104430096B CN 104430096 B CN104430096 B CN 104430096B CN 201410764751 A CN201410764751 A CN 201410764751A CN 104430096 B CN104430096 B CN 104430096B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of Siniperca chuatsi family selective breeding group isolation hatching method, concrete steps include: parent fish copulation, ovum artificial collection fertilization, clean after proceed to hatchery hatching.Being put into by fish roe in the incubation vessel of hatchery, incubation vessel is all independent, when just putting into germ cell, water velocity controls at 7L/min, after 24h, reduce 0.2L/min every 2h water velocity, after more than 85% incubating oosperm membrane, increase 0.3L/min every 1h water velocity；After fry hatches, it is put into collecting seedling box.The present invention meets the demand of hatch fish roe environment, makes germ cell Rapid development, shortens natural brooding time, and survival rate is high.Achieve the isolation of Siniperca chuatsi family selective breeding, big group, synchronize hatching, make the hatching of Siniperca chuatsi family not restricted by traditional incubation facility condition.
Description
Technical field
The present invention relates to larviculture technology field, specifically one Siniperca chuatsi family selective breeding group isolation hatching method.
Background technology
The famous and precious Fish of Gui Shi China tradition fresh water, its fine and tender taste, nutritious, for top grade in fish, there is the highest edible, economic, ecological and scientific research value.In the recent decade, owing to inland natural water area suffers pollution in various degree, rivers and lakes fishery excessive amount of fishing, in addition people are to the understanding deficiency of bio-diversity, inbreeding etc., Siniperca chuatsi resource receives serious threat, Siniperca chuatsi seed premunition is decreased obviously, and the Siniperca chuatsi speed of growth is slow, cultivation survival rate is low.Therefore, carry out Siniperca chuatsi high-quality stock breeding and new varieties, the development to propelling Siniperca chuatsi industrialization, improves culture benefit significant.
Family breeding is the traditional method of fine-variety breeding, but is also to compare accreditation and the fish breeding means praised highly the most in the world.In family breeding, problem being most difficult to is that to be available for the different family groups quantity of selection-breeding a lot, and require that between family, energy is separate and synchronizes realization breeding, to distinguish pedigree (parent and the relation of filial generation), often cannot meet this process because of conventional facilities and propagation method so that family selective breeding lacks enough data.
Summary of the invention
It is an object of the invention to provide a kind of can meet Siniperca chuatsi family selective breeding artificial incubation synchronicity, independence is good and the time is short, survival rate is high group's isolation hatching method, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
A kind of Siniperca chuatsi family selective breeding group isolation hatching method, concrete steps include:
(1) parent fish combo: by parent's combo scheme of predetermined selection-breeding in net cage, completing the male and female combo of Siniperca chuatsi, every square metre of net cage puts 5-6 kilogram of parent fish, carries out artificial induced spawning when water temperature is more than 25 °；
(2) preparation of hatchery: install incubation vessel in hatchery, and number, line up in-line queue, size according to hatchery arranges number of queues, queue spacing 0.2-0.8m, after connecting the water inlet of incubation vessel, oxygen supplement facility and support bar, opens the water inlet pipe on hatchery, starting water pump, the degree of depth of the hatching water injecting cleaning is 1m；
(3) ovum artificial collection insemination: by predetermined breeding goal combo, full sib or half sib combo is used to adopt ovum insemination, gently extrude out from raun abdominal cavity ovum, seminal fluid is extruded in corresponding milter abdominal cavity, complete sperm ovum binding fertilization, take germ cell 50-100ml, rinsing, proceed to the special incubation vessel in hatchery after removing residue；And the source-information of the mark parent that labels；
(4) hatching: it is 0.8-1.5 ten thousand/liter water that germ cell throws in density；When just putting into germ cell, water velocity controls after 7L/min, 24h, reduces 0.2L/min every 2h water velocity, increases 0.3L/min every 1h water velocity after more than 85% incubating oosperm membrane；After fry hatches, collect from the Ji Miaokou of incubation vessel and be put into collecting seedling box.
As the further scheme of the present invention: the temperature of hatching water controls at 26-30 DEG C, and salinity controls at 1.2-1.6 ‰, and pH is 6.5-8.3, oxygen content 8-10mg/L.
Compared with prior art, the invention has the beneficial effects as follows: the present invention meets the demand of hatch fish roe environment, make germ cell Rapid development, shorten natural brooding time, and survival rate is high.Achieve group's isolation of Siniperca chuatsi family selective breeding, synchronize hatching, make the hatching of Siniperca chuatsi family not restricted by traditional incubation facility condition.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of Siniperca chuatsi family selective breeding group isolation hatching method, concrete steps include:
(1) parent fish combo: by parent's combo scheme of predetermined selection-breeding in net cage, completing the male and female combo of Siniperca chuatsi, every square metre of net cage is put 5 kilograms of parent fishs, carried out artificial induced spawning when water temperature is more than 25 °；
(2) preparation of hatchery: install incubation vessel in hatchery, and number, line up in-line queue, size according to hatchery arranges number of queues, queue spacing 0.2m, after connecting the water inlet of incubation vessel, oxygen supplement facility and support bar, opens the water inlet pipe on hatchery, starting water pump, the degree of depth of the hatching water injecting cleaning is 1m；
(3) ovum artificial collection insemination: by predetermined breeding goal combo, full sib or half sib combo is used to adopt ovum insemination, gently extrude out from raun abdominal cavity ovum, seminal fluid is extruded in corresponding milter abdominal cavity, complete sperm ovum binding fertilization, take germ cell 50ml, rinsing, proceed to the special incubation vessel in hatchery after removing residue；And the source-information of the mark parent that labels；
(4) hatching: the temperature of hatching water controls at 26 DEG C, and salinity controls 1.2 ‰, and pH is 6.5, oxygen content 8mg/L.It is 0.8 ten thousand/liter water that germ cell throws in density；When just putting into germ cell, water velocity controls after 7L/min, 24h, reduces 0.2L/min every 2h water velocity, increases 0.3L/min every 1h water velocity after more than 85% incubating oosperm membrane；After fry hatches, collect from the Ji Miaokou of incubation vessel and be put into collecting seedling box.
Embodiment 2
In the embodiment of the present invention, a kind of Siniperca chuatsi family selective breeding group isolation hatching method, concrete steps include:
(1) parent fish combo: by parent's combo scheme of predetermined selection-breeding in net cage, completing the male and female combo of Siniperca chuatsi, every square metre of net cage is put 5.5 kilograms of parent fishs, carried out artificial induced spawning when water temperature is more than 25 °；
(2) preparation of hatchery: install incubation vessel in hatchery, and number, line up in-line queue, size according to hatchery arranges number of queues, queue spacing 0.5m, after connecting the water inlet of incubation vessel, oxygen supplement facility and support bar, opens the water inlet pipe on hatchery, starting water pump, the degree of depth of the hatching water injecting cleaning is 1m；
(3) ovum artificial collection insemination: by predetermined breeding goal combo, full sib or half sib combo is used to adopt ovum insemination, gently extrude out from raun abdominal cavity ovum, seminal fluid is extruded in corresponding milter abdominal cavity, complete sperm ovum binding fertilization, take germ cell 75ml, rinsing, proceed to the special incubation vessel in hatchery after removing residue；And the source-information of the mark parent that labels；
(4) hatching: the temperature of hatching water controls at 28 DEG C, and salinity controls 1.4 ‰, and pH is 7.1, oxygen content 9mg/L.It is 1.15 ten thousand/liter water that germ cell throws in density；When just putting into germ cell, water velocity controls after 7L/min, 24h, reduces 0.2L/min every 2h water velocity, increases 0.3L/min every 1h water velocity after more than 85% incubating oosperm membrane；After fry hatches, collect from the Ji Miaokou of incubation vessel and be put into collecting seedling box.
Embodiment 3
In the embodiment of the present invention, a kind of Siniperca chuatsi family selective breeding group isolation hatching method, concrete steps include:
(1) parent fish combo: by parent's combo scheme of predetermined selection-breeding in net cage, completing the male and female combo of Siniperca chuatsi, every square metre of net cage is put 6 kilograms of parent fishs, carried out artificial induced spawning when water temperature is more than 25 °；
(2) preparation of hatchery: install incubation vessel in hatchery, and number, line up in-line queue, size according to hatchery arranges number of queues, queue spacing 0.8m, after connecting the water inlet of incubation vessel, oxygen supplement facility and support bar, opens the water inlet pipe on hatchery, starting water pump, the degree of depth of the hatching water injecting cleaning is 1m；
(3) ovum artificial collection insemination: by predetermined breeding goal combo, full sib or half sib combo is used to adopt ovum insemination, gently extrude out from raun abdominal cavity ovum, seminal fluid is extruded in corresponding milter abdominal cavity, complete sperm ovum binding fertilization, take germ cell 100ml, rinsing, proceed to the special incubation vessel in hatchery after removing residue；And the source-information of the mark parent that labels；
(4) hatching: the temperature of hatching water controls at 30 DEG C, and salinity controls 1.6 ‰, and pH is 8.3, oxygen content 10mg/L.It is 1.5 ten thousand/liter water that germ cell throws in density；When just putting into germ cell, water velocity controls after 7L/min, 24h, reduces 0.2L/min every 2h water velocity, increases 0.3L/min every 1h water velocity after more than 85% incubating oosperm membrane；After fry hatches, collect from the Ji Miaokou of incubation vessel and be put into collecting seedling box.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and without departing from the spirit or essential characteristics of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is limited by claims rather than described above, it is intended that all changes fallen in the implication of equivalency and scope of claim included in the present invention.
In addition, it is to be understood that, although this specification is been described by according to embodiment, but the most each embodiment only comprises an independent technical scheme, this narrating mode of description is only for clarity sake, description should can also be formed, through appropriately combined, other embodiments that it will be appreciated by those skilled in the art that as an entirety, the technical scheme in each embodiment by those skilled in the art.
Claims (1)
1. a Siniperca chuatsi family selective breeding group isolation hatching method, it is characterised in that concrete steps include:
(1) parent fish combo: by parent's combo scheme of predetermined selection-breeding in net cage, the male and female completing Siniperca chuatsi are joined
Group, every square metre of net cage is put 5-6 kilogram of parent fish, is carried out artificial induced spawning when water temperature is more than 25 DEG C；
(2) preparation of hatchery: install incubation vessel in hatchery, and number, line up in-line queue,
Size according to hatchery arranges number of queues, queue spacing 0.2-0.8m, connects the water inlet of incubation vessel, increasing
After oxygen facility and support bar, opening the water inlet pipe on hatchery, start water pump, that injects cleaning hatches the deep of water
Degree is 1m；
(3) ovum artificial collection insemination: by predetermined breeding goal combo, uses full sib or half sib combo to adopt ovum
Insemination, ovum of gently extruding out from raun abdominal cavity, seminal fluid is extruded in corresponding milter abdominal cavity, completes sperm ovum binding fertilization,
Take germ cell 50-100m1, rinsing, proceed to the special incubation vessel in hatchery after removing residue；And labeling
Sign the source-information of mark parent；
(4) hatching: the temperature of described hatching water controls at 25-30 DEG C, and salinity controls at 1.2-1.6 ‰, pH
For 6.5-8.3, oxygen content 8-10mg/L；It is 0.8-1.5 ten thousand/liter water that germ cell throws in density；Just put into and be subject to
During essence ovum, water velocity controls after 7L/min, 24h, reduces 0.2L/min every 2h water velocity, treats
0.3L/min is increased every 1h water velocity after more than 85% incubating oosperm membrane；After fry hatches, from incubating
The Ji Miaokou changing container collects and is put into collecting seedling box.
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| CN105123560B (en) * | 2015-07-14 | 2018-01-02 | 神农架林区佳园科技养殖有限公司 | The mating system of red tail pair loach fry and the incubator for its incubating oosperm |
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| 提高鳜鱼产卵率、孵化率和苗种培育成活率的几条措施;钱勇 等;《水产科技情报》;20080430;第35卷(第2期);第80-81页 * |
| 斑鳜的人工繁殖及网箱养殖技术;李红敬 等;《信阳农业高等专科学校学报》;20110331;第21卷(第1期);第116-118页 * |
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